Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| guidelines for the direct detection of anaplasma spp. in diagnosis and epidemiological studies. | the genus anaplasma (rickettsiales: anaplasmataceae) comprises obligate intracellular gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: anaplasma bovis, anaplasma centrale, anaplasma marginale, anaplasma phagocytophilum, anaplasma platys, and anaplasma ovis. these have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. a zoonotic role has been recognized for a. phagocytophilum, but other spe ... | 2017 | 28055579 |
| comparison of three nucleic acid-based tests for detecting <i>anaplasma marginale</i> and <i>anaplasma centrale</i> in cattle. | several nucleic acid-based assays have been developed for detecting anaplasma marginale and anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. we evaluated the ability of the reverse line blot (rlb) hybridisation assay, two nested polymerase chain reaction (npcr) assays and a duplex real-time quantitative polymerase chain reaction (qpcr) assay to detect a. marginale and a. centrale infections in cattle (n = 66) in south africa. the lowest dete ... | 2017 | 28155283 |
| identification of a t-cell epitope that is globally conserved among outer membrane proteins (omps) omp7, omp8, and omp9 of anaplasma marginale strains and with omp7 from the a. marginale subsp. centrale vaccine strain. | within the protective outer membrane (om) fraction of anaplasma marginale, several vaccine candidates have emerged, including a family of om proteins (omps) 7 to 9, which share sequence identity with each other and with the single protein omp7 in the vaccine strain a. marginale subsp. centrale. a. marginale omps 7 to 9 are logical vaccine candidates because they are surface exposed, present in the om immunogen and protective cross-linked om proteins, recognized by immune serum igg2 and t cells i ... | 2017 | 27795302 |
| parasites of domestic and wild animals in south africa. l. ixodid ticks infesting horses and donkeys. | the aim of the study was to determine the species spectrum of ixodid ticks that infest horses and donkeys in south africa and to identify those species that act as vectors of disease to domestic livestock. ticks were collected opportunistically from 391 horses countrywide by their owners or grooms, or by veterinary students and staff at the faculty of veterinary science, university of pretoria. ticks were also collected from 76 donkeys in limpopo province, 2 in gauteng province and 1 in north we ... | 2017 | 28281774 |
| molecular characterisation of anaplasma species from african buffalo (syncerus caffer) in kruger national park, south africa. | bovine anaplasmosis is a tick-borne disease, mainly caused by anaplasma marginale and a. centrale and is distributed in tropical and sub-tropical areas. this study aimed to characterise a. marginale and a. centrale from african buffaloes in kruger national park (knp), south africa, using the dna sequences of the genes coding for major surface protein (msp1β) and heat shock protein (groel), respectively. a total of 747 blood samples were collected from february 2014 to august 2016 from african bu ... | 2017 | 28169172 |
| genetic diversity and molecular epidemiology of anaplasma. | anaplasma are obligate intracellular bacteria of cells of haematopoietic origin and are aetiological agents of tick-borne diseases of both veterinary and medical interest common in both tropical and temperate regions. the recent disclosure of their zoonotic potential has greatly increased interest in the study of these bacteria, leading to the recent reorganisation of rickettsia taxonomy and to the possible discovery of new species belonging to the genus anaplasma. this review is particularly fo ... | 2017 | 28122249 |
| molecular detection and genetic diversity of bovine babesia spp., theileria orientalis, and anaplasma marginale in beef cattle in thailand. | babesia spp., theileria orientalis, and anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. in this study, we used pcr to detect the presence of babesia bovis, babesia bigemina, and t. orientalis in 279 beef cattle from western thailand and a. marginale in 608 beef cattle from the north, northeastern, and western regions. the pcrs were performed using species-specific primers based on the b. bovis spherical ... | 2017 | 28028631 |
| anaplasma marginale outer membrane protein a is an adhesin that recognizes sialylated and fucosylated glycans and functionally depends on an essential binding domain. | anaplasma marginale causes bovine anaplasmosis, a debilitating and potentially fatal tick-borne infection of cattle. because a. marginale is an obligate intracellular organism, its adhesins that mediate entry into host cells are essential for survival. here, we demonstrate that a. marginale outer membrane protein a (amompa; am854) contributes to the invasion of mammalian and tick host cells. amompa exhibits predicted structural homology to ompa of a. phagocytophilum (apompa), an adhesin that use ... | 2017 | 27993973 |
| parasitological and molecular surveys reveal high rates of infection with vector-borne pathogens and clinical anemia signs associated with infection in cattle from two important livestock areas in colombia. | in colombia, vector-borne diseases are one of the most important problems in the livestock industry. the present study reports parasitological and molecular surveys of vector-borne pathogens in cattle from two high-value livestock areas in colombia. a total of 464 samples (226 from antioquia and 238 from arauca) were analyzed. while the blood smear analysis identified 98 (21.1%), 14 (3.0%) and 30 (6.5%) positive samples for anaplasma spp., babesia spp. and trypanosoma spp., respectively, the mol ... | 2017 | 27956049 |
| phylogenetic relationship and genotypic variability in anaplasma marginale strains causing anaplasmosis in india. | anaplasma marginale is a tick borne rickesttsial parasite known to cause bovine anaplasmosis. there are prevalence reports from different parts of india, however, information regarding genetic diversity and phylogenetic association of the indian strains are unknown. in the current study, 965 cattle blood samples from two states of india, seemandhra and telangana, were investigated for the presence of a. marginale by pcr using major surface protein 4 gene (msp4). we found an overall infection of ... | 2017 | 27939332 |
| a partially purified outer membrane protein virb9-1 for low-cost nanovaccines against anaplasma marginale. | anaplasma marginale is a devastating tick-borne pathogen causing anaplasmosis in cattle and results in significant economic loss to the cattle industry worldwide. currently, there is no widely accepted vaccine against a. marginale. new generation subunit vaccines against a. marginale, which are much safer, more efficient and cost-effective, are in great need. the a. marginale outer membrane protein virb9-1 is a promising antigen for vaccination. we previously have shown that soluble recombinant ... | 2017 | 27890399 |
| seroprevalence of anaplasma marginale and babesia bigemina infections and associated risk factors in machakos county, kenya. | anaplasma marginale and babesia bigemina are important tick-borne pathogens of cattle. a cross-sectional survey was undertaken to determine the seroprevalence of a. marginale and b. bigemina infections and identify associated risk factors on traditional smallholder farms in machakos county, kenya. a total of 421 cattle from 127 farms from four divisions in the county were sampled and visited between september and november 2007. the farms were selected by a proportional allocation approach based ... | 2017 | 27878428 |
| a comparison of dna extraction protocols from blood spotted on fta cards for the detection of tick-borne pathogens by reverse line blot hybridization. | an essential step in the molecular detection of tick-borne pathogens (tbps) in blood is the extraction of dna. when cooled storage of blood under field conditions prior to dna extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. we evaluated six dna extraction methods from blood spotted on fta classic(®) cards (fta cards), to determine the optimal protocol for the subsequent molecular detection of tbps by pcr and the reverse li ... | 2017 | 27825733 |
| seroprevalence of babesia bovis, b. bigemina, trypanosoma evansi, and anaplasma marginale antibodies in cattle in southern egypt. | babesia bovis, b. bigemina, trypanosoma evansi, and anaplasma marginale infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for b. bovis, b. bigemina, and a. marginale and various types of flies for t. evansi). in the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern egypt, but no accurate or adequate data are available for the southern regions. therefore, in this study, we screened for ... | 2017 | 27789159 |
| molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france. | corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ... | 2017 | 28645313 |
| assessment of a quantitative 5' nuclease real-time polymerase chain reaction using groel gene for ehrlichia and anaplasma species in rodents in brazil. | new genotypes of anaplasmataceae agents have been detected in wild carnivores, birds and deer in brazil. the present work aimed to investigate the presence of ehrlichia and anaplasma species in rodents sampled in brazil. additionally, a newly designed quantitative 5' nuclease real-time multiplex pcr for ehrlichia and anaplasma spp. detection based on groel gene amplification was designed, showing high specificity and sensitivity (10 groel fragment copy/μl). between 2000 and 2011, different roden ... | 2017 | 28457822 |
| seasonal prevalence of blood parasitic diseases in crossbred cattle of mysore and its surrounding districts of karnataka. | the present study report the seasonal prevalence of blood parasitic diseases in cross bred cattle in mysore and its surrounding districts of karnataka such as mandya, chamaraj nagar and kodagu. the study was undertaken for a period of 2 years from august 2013 to july 2015. a total of 1655 blood samples were collected from clinically suspected cattle for blood parasitic diseases with clinical symptoms of anorexia, high fever, anaemia, salivation, enlargement of superficial lymphnodes, haemoglobin ... | 2017 | 28848277 |
| rhipicephalus microplus and dermacentor nitens (acari: ixodidae) coparasitize white-tailed deer on st. john, u.s. virgin islands. | ticks parasitizing introduced white-tailed deer, odocoileus virginianus (zimmermann), on st. john, u.s. virgin islands, were recorded during and after drought conditions. tick infestation prevalences were 22% at the start of the drought (july 2015), 66% at the height of the drought (march 2016), and 35% after the drought had ended (july 2016; n = 67 deer). samples of ticks from 22 tranquilized deer in july 2016 revealed the presence of two species, the southern cattle tick, rhipicephalus (boophi ... | 2017 | 28591859 |
| validation of an improved anaplasma antibody competitive elisa for detection of anaplasma ovis antibody in domestic sheep. | an accurate and simple-to-perform new version of a competitive elisa (celisa) kit that became commercially available in 2015 for testing of cattle for antibody to anaplasma marginale was validated for detection of anaplasma ovis antibody in domestic sheep. true positives and negatives were identified using nested pcr (npcr) as the gold standard. negative bovine control sera supplied with the kit were used to calculate % inhibition (%i), designated bovine control elisa (bcelisa), and this was com ... | 2017 | 28480803 |
| the transcription factor relish controls anaplasma marginale infection in the bovine tick rhipicephalus microplus. | rhipicephalus microplus is an important biological vector of anaplasma marginale, the etiological agent of bovine anaplasmosis. the knowledge of tick immune responses to control bacterial infections remains limited. in this study, we demonstrate that transcription factor relish from the imd signaling pathway has an important role in the control of a. marginale infection in ticks. we found that rna-mediated silencing of relish caused a significant increase in the number of a. marginale in the mid ... | 2017 | 28408334 |
| a nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid a and recombinant mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages. | we evaluated the potential of a nanoparticle (np) delivery system to improve methods of delivery of candidate peptide-based vaccines for paratuberculosis in cattle. | 2017 | 28502107 |
| molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock. | anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ... | 2017 | 28678004 |
| molecular detection and genetic identification of babesia bigemina, theileria annulata, theileria orientalis and anaplasma marginale in turkey. | babesia spp., theileria spp. and anaplasma spp. are significant tick-borne pathogens of livestock globally. in this study, we investigated the presence and distribution of babesia bigemina, theileria annulata, theileria orientalis and anaplasma marginale in cattle from 6 provinces of turkey using species-specific pcr assays. the pcr were conducted using the primers based on the b. bigemina rhoptry-associated protein 1a (bbirap-1a), t. annulata merozoite surface antigen-1 (tams-1), t. orientalis ... | 2016 | 26492823 |
| low genetic diversity of anaplasma marginale in calves in an endemic area for bovine anaplasmosis in the state of são paulo, brazil. | major surface protein 1a has been used as a marker for genetic stability in identifying geographical isolates of anaplasma marginale. we conducted a longitudinal study to ascertain the genetic diversity of a. marginale in a dairy cattle herd with a history of clinical anaplasmosis. a total of 20 calves were evaluated every 3 months, from birth to 1 year of age. they were evaluated using blood smears, ifat, elisa, and qpcr. additionally, samples positive for the msp1a gene using npcr were sequenc ... | 2016 | 26318264 |
| first molecular survey and identification of anaplasma spp. in white yaks (bos grunniens) in china. | anaplasmosis is caused by a group of obligate intracellular bacteria in the genus anaplasma, which are transmitted by ticks and infect humans, domestic animals and wildlife. this study was conducted to determine the prevalence and molecular characterization of anaplasma spp. in semi-wild white yaks sampled in tianzhu tibetan autonomous county, northwest china. out of 332 samples tested, 35 (10·9%) were positive for anaplasma spp. the positive rates were 6·2% (20/322) and 5·3% (17/322) for anapla ... | 2016 | 27003378 |
| type-specific pcr assays for babesia bovis msa-1 genotypes in asia: revisiting the genetic diversity in sri lanka, mongolia, and vietnam. | babesia bovis is the most virulent babesia organism, resulting in a high mortality rate in cattle. the genetic diversity of b. bovis merozoite surface antigens (msas), such as msa-1, msa-2b, and msa-2c, might be linked to altered immune profiles in the host animals. the present study aimed to develop type-specific pcr assays for asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in sri lanka, mongolia, and vietnam. specific primers were designed for nine asian msa-1 genot ... | 2016 | 26520797 |
| detection and phylogenetic characterization of theileria spp. and anaplasma marginale in rhipicephalus bursa in portugal. | ticks are obligatory blood-sucking arthropod (acari:ixodida) ectoparasites of domestic and wild animals as well as humans. the incidence of tick-borne diseases is rising worldwide, challenging our approach toward diagnosis, treatment and control options. rhipicephalus bursa canestrini and fanzago, 1877, a two-host tick widely distributed in the palearctic mediterranean region, is considered a multi-host tick that can be commonly found on sheep, goats and cattle, and occasionally on horses, dogs, ... | 2016 | 26797395 |
| persistent infections and immunity in ruminants to arthropod-borne bacteria in the family anaplasmataceae. | tick-transmitted gram-negative bacteria in the family anaplasmataceae in the order rickettsiales cause persistent infection and morbidity and mortality in ruminants. whereas anaplasma marginale infection is restricted to ruminants, anaplasma phagocytophilum is promiscuous and, in addition to causing disease in sheep and cattle, notably causes disease in humans, horses, and dogs. although the two pathogens invade and replicate in distinct blood cells (erythrocytes and neutrophils, respectively), ... | 2016 | 26734888 |
| exploring the immune signalling pathway-related genes of the cattle tick rhipicephalus microplus: from molecular characterization to transcriptional profile upon microbial challenge. | in dipteran insects, invading pathogens are selectively recognized by four major pathways, namely toll, imd, jnk, and jak/stat, and trigger the activation of several immune effectors. although substantial advances have been made in understanding the immunity of model insects such as drosophila melanogaster, knowledge on the activation of immune responses in other arthropods such as ticks remains limited. herein, we have deepened our understanding of the intracellular signalling pathways likely t ... | 2016 | 26724380 |
| nanoparticle-based delivery of anaplasma marginale membrane proteins; virb9-1 and virb10 produced in the pichia pastoris expression system. | bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite anaplasma marginale in tropical and subtropical areas of the world. while difficult to express, the proteins virb9-1 and virb10 are immunogenic components of the outer membrane type iv secretion system that have been identified as candidate antigens for vaccines targeting of a. marginale. soluble virb9-1 and virb10 were successfully expressed using pichia pastoris. when formulated ... | 2016 | 28335329 |
| identification of a vertically transmitted strain from anaplasma marginale (ufmg3): molecular and phylogenetic characterization, and evaluation of virulence. | bovine anaplasmosis is a disease caused by the intraerythrocytic rickettsia species anaplasma marginale and results in great economic losses in tropical and subtropical regions. vertical transmission is an important phenomenon that contributes to the persistence of different strains of the agent within the same herd. the identification of new strains and genetic characterization studies are essential to understanding their epidemiology and virulence and for vaccine development. the aim of this s ... | 2016 | 26381444 |
| survey of ticks collected from tennessee cattle and their pastures for anaplasma and ehrlichia species. | anaplasma marginale is the causative agent for bovine anaplasmosis (ba) and ehrlichia ruminantium is the causative agent for heartwater, 2 devastating diseases of cattle. ba is common in the united states and frequently reported in western tennessee cattle; however, cases of heartwater are not yet established in the continental united states. because both pathogens are transmitted via the bites of infected ticks, the objective of this study was to survey cattle and pastures for ticks and for eac ... | 2016 | 26348980 |
| investigation of anaplasma marginale seroprevalence in a traditionally managed large california beef herd. | recent observations by stakeholders suggested that ecosystem changes may be driving an increased incidence of bovine erythrocytic anaplasmosis, resulting in a reemerging cattle disease in california. the objective of this prospective cohort study was to estimate the incidence of anaplasma marginale infection using seroconversion in a northern california beef cattle herd. a total of 143 black angus cattle (106 prebreeding heifers and 37 cows) were enrolled in the study. serum samples were collect ... | 2016 | 27656312 |
| transplacental transmission of bovine tick-borne pathogens: frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of brazil. | bovine tick-borne disease (tbd) constitutes a worldwide group of diseases that result in great losses for dairy and beef cattle. with regard to the epidemiological profile of the diseases, the importance of transplacental transmission is still not very well understood. the aim of this study was to determine the transplacental transmission of tbd agents (anaplasma marginale, babesia bovis and b. bigemina) in a herd of dairy cattle that had been naturally infected in an area of enzootic stability ... | 2016 | 26613663 |
| bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from bahia, brazil. | direct diagnoses were made by using - blood smears and nested pcr (npcr) tests on 309 blood samples from crossbred dairy cattle in the municipality of ibicaraí, bahia. from diagnostic blood smear slides, the observed parasitic frequencies were 31.1% for anaplasma marginale and 20.4% for babesia sp. from npcr diagnoses, they were 63% for a. marginale, 34% for babesia bigemina and 20.4% for babesia bovis. there were significant differences (p <0.01) between the two diagnostic methods (npcr and blo ... | 2016 | 25271452 |
| molecular diagnosis of the tick-borne pathogen anaplasma marginale in cattle blood samples from nigeria using qpcr. | tick-borne diseases (tbds) are some of the most important animal health and management problems in africa, including nigeria. this study aims to determine the prevalence of an important tbd, anaplasmosis, in a north-central region of nigeria. blood samples were collected from cattle and stored on whatman fta(®) cards. information on village, age and sex associated with each cattle was also recorded. the packed red blood cell volume (pcv) for each blood sample was determined. after dna extraction ... | 2016 | 27787614 |
| anaplasma marginale and a. phagocytophilum in cattle in tunisia. | tick-borne diseases caused by anaplasma species put serious constraints on the health and production of domestic cattle in tropical and sub-tropical regions. after recovering from a primary infection, cattle typically become persistent carriers of pathogens and play a critical role in the epidemiology of the disease, acting as reservoirs of the anaplasma spp. | 2016 | 27765073 |
| enzootic instability for bovine anaplasmosis on family farms located in southwestern paraná, brazil. | the objective of this study was to assess the occurence of animals seropositive for anaplasma marginale in the municipality of realeza, paraná state, brazil. blood samples were collected from 344 cows on 18 small farms in the municipality of realeza-pr. the animals'serum samples were forwarded to the federal university of fronteira do sul, in order to investigate the occurrence of anti-a. marginale igg antibodies by an enzyme-linked immunosorbent assay commercial kit. igg antibodies to a. margin ... | 2016 | 27759758 |
| isolation and attempted cultivation of an anaplasma marginale strain from brazilian brown brocket deer (mazama gouazoubira, fisher, 1814) in the tick cell line ide8. | the aim of the study was to isolate and establish an anaplasma marginale strain from brazilian brown brocket deer, mazama gouazoubira, in the ixodes scapularis cell line ide8. blood from a free-living adult female m. gouazoubira naturally infected with a. marginale (mgi5) was inoculated intravenously into a splenectomized calf. when a. marginale rickettsemia was 2.5%, blood was collected and cryopreserved in liquid nitrogen with dimethylsulfoxide (dmso). ide8 cell cultures were infected with cal ... | 2016 | 27612925 |
| prevalence of haemoprotozoan infections in bovines of shimoga region of karnataka state. | a study was conducted to record the prevalence of haemoprotozoan infections in bovines of shimoga region for a period of 1 year from april 2012 to march 2013. a total of 300 blood samples were examined for the presence of haemoprotozoan parasites, of which 215 from cattle and 85 from buffaloes were examined by giemsa staining technique. out of 300 blood samples examined, 130 (43.3 %) were found positive for haemoprotozoan infections. out of 215 cattle blood samples examined, 62 (28.8 %) were pos ... | 2016 | 27605804 |
| prevalence and genetic characterization of anaplasma marginale in zebu cattle (bos indicus) and their ticks (amblyomma variegatum, rhipicephalus microplus) from madagascar. | tick-borne bovine anaplasmosis, caused by the obligate intracellular pathogen anaplasma marginale (rickettsiales: anaplasmataceae), is a major constraint to cattle production in tropical and subtropical regions. from madagascar, clinical cases were published but data based on molecular methods regarding the prevalence and genetic diversity of this pathogen on the island are lacking. the aims of this study were to investigate (1) the prevalence of a. marginale in malagasy zebu cattle (bos indicus ... | 2016 | 27592064 |
| molecular study on infection rates of anaplasma ovis and anaplasma marginale in sheep and cattle in west-azerbaijan province, iran. | this study was carried out to determine the presence and frequency of anaplasma ovis and anaplasma marginale in sheep and dairy cattle in west-azerbaijan province, iran. a total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). the extracted dna from blood cells was screened using genus-specific (anaplasma spp.) nested-polymerase chain reaction (pcr) based on 16s rrna gene primer sets. species-specific pcr was set up us ... | 2016 | 27482362 |
| cooperation of pd-1 and lag-3 contributes to t-cell exhaustion in anaplasma marginale-infected cattle. | the cd4(+) t-cell response is central for the control of anaplasma marginale infection in cattle. however, the infection induces a functional exhaustion of antigen-specific cd4(+) t cells in cattle immunized with a. marginale outer membrane proteins or purified outer membranes (oms), which presumably facilitates the persistence of this rickettsia. in the present study, we hypothesize that t-cell exhaustion following infection is induced by the upregulation of immunoinhibitory receptors on t cell ... | 2016 | 27430272 |
| structural basis for recombinatorial permissiveness in the generation of anaplasma marginale msp2 antigenic variants. | sequential expression of outer membrane protein antigenic variants is an evolutionarily convergent mechanism used by bacterial pathogens to escape host immune clearance and establish persistent infection. variants must be sufficiently structurally distinct to escape existing immune effectors yet retain the core structural elements required for localization and function within the outer membrane. we examined this balance using anaplasma marginale, which generates antigenic variants in the outer m ... | 2016 | 27400719 |
| erratum to: molecular identification of anaplasma marginale in two autochthonous south american wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines. | 2016 | 27341801 | |
| imidocarb dipropionate in the treatment of anaplasma marginale in cattle: effects on enzymes of the antioxidant, cholinergic, and adenosinergic systems. | anaplasmosis is a worldwide hemolytic disease in cattle caused by a gram-negative obligatory intracellular bacterium, characterized by anemia and jaundice. among the treatments used for anaplasmosis is a drug called imidocarb dipropionate, also indicated as an immunomodulator agent. however, it causes side effects associated with increased levels of acetylcholine. in view of this, the effects of imidocarb dipropionate on the purinergic system, and antioxidant enzymes in animals naturally infecte ... | 2016 | 27301742 |
| repeatanalyzer: a tool for analysing and managing short-sequence repeat data. | short-sequence repeats (ssrs) occur in both prokaryotic and eukaryotic dna, inter- and intragenically, and may be exact or inexact copies. when heterogeneous ssrs are present in a given locus, we can take advantage of the pattern of different repeats to genotype strains based on the ssrs. cataloguing and tracking these repeats can be difficult as diverse groups of researchers are involved in the identification of the repeats. additionally, the task is error-prone when done manually. | 2016 | 27260942 |
| immunogenicity of outer membrane proteins virb9-1 and virb9-2, a novel nanovaccine against anaplasma marginale. | anaplasma marginale is the most prevalent tick-borne livestock pathogen and poses a significant threat to cattle industry. in contrast to currently available live blood-derived vaccines against a. marginale, alternative safer and better-defined subunit vaccines will be of great significance. two proteins (virb9-1 and virb9-2) from the type iv secretion system of a. marginale have been shown to induce humoral and cellular immunity. in this study, escherichia coli were used to express virb9-1 and ... | 2016 | 27115492 |
| cattle experimentally infected by anaplasma marginale: influence of splenectomy on disease pathogenesis, oxidative profile, and antioxidant status. | bovine anaplasmosis is caused by the obligate intraerythrocytic bacteria anaplasma marginale. these bacteria are transmitted by tick species such as rhipicephalus (boophilus) microplus, blood-sucking insects, and fomites (needles, clippers, and other blood contaminated equipment). during the acute phase of infection, animals may develop fever, anemia, jaundice, and hepatosplenomegaly. the aims of this study are to quantify the bacteremia by quantitative pcr in eight naïve calves experimentally i ... | 2016 | 27060744 |
| influence of experimental anaplasma marginale infection and splenectomy on ntpdase and 5'nucleotidase activities in platelets of cattle. | the objective of this paper was to evaluate ntpdase and 5'-nucleotidase activities in platelets of bovine with and without spleen and infected by anaplasma marginale. our results demonstrate that infection along with splenectomy is able of inducing a profile of cellular protection, which showed an increase in the degradation of the nucleotides atp and adp by ntpdase, in addition to amp by 5'nucleotidase to form the nucleoside adenosine in platelets, i.e., the enzymatic activities of platelets we ... | 2016 | 26945560 |
| molecular screening for anaplasmataceae in ticks and tsetse flies from ethiopia. | hard ticks and tsetse flies are regarded as the most important vectors of disease agents in sub-saharan africa. with the aim of screening these blood-sucking arthropods for vector-borne pathogens belonging to the family anaplasmataceae in south-western ethiopia, four species of tsetse flies (collected by traps) and seven species of ixodid ticks (removed from cattle) were molecularly analysed. dna was extracted from 296 individual ticks and from 162 individuals or pools of tsetse flies. besides k ... | 2016 | 26919143 |
| the bacterial microbiome of dermacentor andersoni ticks influences pathogen susceptibility. | ticks are of medical importance owing to their ability to transmit pathogens to humans and animals. the rocky mountain wood tick, dermacentor andersoni, is a vector of a number of pathogens, including anaplasma marginale, which is the most widespread tick-borne pathogen of livestock. although ticks host pathogenic bacteria, they also harbor bacterial endosymbionts that have a role in tick physiology, survival, as well as pathogen acquisition and transmission. the goal of this study was to charac ... | 2016 | 26882265 |
| seasonal incidence of parasitic diseases in bovines of south western gujarat (junagadh), india. | the current study was conducted to investigate the incidence of parasitic diseases in bovines which were sick and brought at veterinary hospital for treatment. a total of 366 samples were investigated from cattle (n = 175) and buffaloes (n = 191) presented at teaching veterinary clinical complex (tvcc), veterinary college, junagadh, gujarat during january to december 2014. examination of giemsa-stained peripheral blood smears exhibited that 58.6 % of cattle and 41.2 % of buffaloes were infected ... | 2016 | 27876944 |
| characterization of anaplasma marginale subsp. centrale strains by use of msp1as genotyping reveals a wildlife reservoir. | bovine anaplasmosis caused by the intraerythrocytic rickettsial pathogen anaplasma marginale is endemic in south africa. anaplasma marginale subspecies centrale also infects cattle; however, it causes a milder form of anaplasmosis and is used as a live vaccine against a. marginale there has been less interest in the epidemiology of a. marginale subsp. centrale, and, as a result, there are few reports detecting natural infections of this organism. when detected in cattle, it is often assumed that ... | 2016 | 27440819 |
| anaplasma marginale and theileria annulata in questing ticks from portugal. | ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ... | 2016 | 27394441 |
| anaplasmataceae in wild ungulates and carnivores in northern spain. | wild vertebrates are essential hosts for tick-borne diseases but data on the prevalence and diversity of anaplasma spp. in wildlife are scarce. in this study, we used real-time pcr to investigate the distribution of anaplasma species in spleen samples collected from 625 wild animals (137 cervids, 227 wild boar, and 261 carnivores) in two regions in northern spain. a first generic real-time pcr assay was used to screen for the presence of anaplasma spp. followed by a second species-specific multi ... | 2016 | 26596894 |
| anaplasma marginale: diversity, virulence, and vaccine landscape through a genomics approach. | in order to understand the genetic diversity of a. marginale, several efforts have been made around the world. this rickettsia affects a significant number of ruminants, causing bovine anaplasmosis, so the interest in its virulence and how it is transmitted have drawn interest not only from a molecular point of view but also, recently, some genomics research have been performed to elucidate genes and proteins with potential as antigens. unfortunately, so far, we still do not have a recombinant a ... | 2016 | 27610385 |
| anaplasma marginale actively modulates vacuolar maturation during intracellular infection of its tick vector, dermacentor andersoni. | tick-borne transmission of bacterial pathogens in the order rickettsiales is responsible for diverse infectious diseases, many of them severe, in humans and animals. transmission dynamics differ among these pathogens and are reflected in the pathogen-vector interaction. anaplasma marginale has been shown to establish and maintain infectivity within dermacentor spp. for weeks to months while escaping the complex network of vacuolar peptidases that are responsible for digestion of the tick blood m ... | 2016 | 27235428 |
| the pathogen-occupied vacuoles of anaplasma phagocytophilum and anaplasma marginale interact with the endoplasmic reticulum. | the genus anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. a. phagocytophilum, a human and veterinary pathogen, infects neutrophils to cause granulocytic anaplasmosis. a. marginale invades bovine erythrocytes. evidence suggests that both species may also infect endothelial cells in vivo. in mammalian and arthropod host cells, a. phagocytophilum and a. marginale reside in host cell d ... | 2016 | 26973816 |
| tick-borne pathogens of zoonotic and veterinary importance in nigerian cattle. | ticks and tick-borne diseases undermine cattle fitness and productivity in the whole of sub-saharan africa, including nigeria. in this west african country, cattle are challenged by numerous tick species, especially during the wet season. consequently, several tbds are known to be endemic in nigerian cattle, including anaplasmosis, babesiosis, cowdriosis and theilerioris (by theileria mutans and theileria velifera). to date, all investigations on cattle tbds in nigeria have been based on cytolog ... | 2016 | 27090756 |
| superinfection exclusion of the ruminant pathogen anaplasma marginale in its tick vector is dependent on the time between exposures to the strains. | the remarkable genetic diversity of vector-borne pathogens allows for the establishment of superinfection in the mammalian host. to have a long-term impact on population strain structure, the introduced strains must also be transmitted by a vector population that has been exposed to the existing primary strain. the sequential exposure of the vector to multiple strains frequently prevents establishment of the second strain, a phenomenon termed superinfection exclusion. as a consequence, superinfe ... | 2016 | 26994084 |
| molecular diagnosis and genetic diversity of tick-borne anaplasmataceae agents infecting the african buffalo syncerus caffer from marromeu reserve in mozambique. | tick-borne diseases (tbds) are very important in relation to domestic ruminants, but their occurrence among wild ruminants, mainly in the african buffalo syncerus caffer, remains little known. | 2016 | 27531003 |
| molecular identification of anaplasma marginale in two autochthonous south american wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines. | anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in myrmecophaga tridactyla (giant anteater) or hippocamelus antisense (taruca), which are two native endangered species. | 2016 | 27229471 |
| predicting audiovisual consonant recognition performance of hearing-impaired adults. | 2016 | 4836044 | |
| multiple pathogens including potential new species in tick vectors in côte d'ivoire. | our study aimed to assess the presence of different pathogens in ticks collected in two regions in côte d'ivoire. | 2016 | 26771308 |
| preoperative prostatectomy preparation by exchange transfusion to reduce circulating anticoagulating antibodies. | 2016 | 5010713 | |
| [an evaluation of the most commonly used serological tests in non-venereal bacterial infections]. | 2016 | 5021891 | |
| [60th anniversary of primarius emeritus dr. leonard rektor]. | 2016 | 4920986 | |
| effects of some viruses on ciliated cells. | 2016 | 4959345 | |
| [remarks on the creative age]. | 2016 | 4896419 | |
| a comparative study of two antihistamines. | 2016 | 4891823 | |
| [blood transfusion in the sovier army and naval fleet over the past 50 years]. | 2016 | 4882842 | |
| comparative study of a hypnotic and a tranquillizer in anxiety. | 2016 | 4893720 | |
| [a study of some problems of clinical allergology in the polish people's republic]. | 2016 | 4862140 | |
| molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from portugal. | wildlife can act as reservoir of different tick-borne pathogens, such as bacteria, parasites and viruses. the aim of the present study was to assess the presence of tick-borne bacteria and protozoa with veterinary and zoonotic importance in cervids and wild boars from the centre and south of portugal. | 2016 | 27160767 |
| disaggregating tropical disease prevalence by climatic and vegetative zones within tropical west africa. | tropical infectious disease prevalence is dependent on many socio-cultural determinants. however, rainfall and temperature frequently underlie overall prevalence, particularly for vector-borne diseases. as a result these diseases have increased prevalence in tropical as compared to temperate regions. specific to tropical africa, the tendency to incorrectly infer that tropical diseases are uniformly prevalent has been partially overcome with solid epidemiologic data. this finer resolution data is ... | 2016 | 27022740 |
| [quantitative cytological bone marrow assessment as a base for the evaluation of the regeneration course after therapeutic x-ray irradiation]. | 2016 | 5035442 | |
| [from the history of organization of public health in the far east]. | 2016 | 4918789 | |
| [selection of the optimal regime of controlled breathing for patients who have undergone surgery in the trendelenburg position]. | 2016 | 4888597 | |
| [contemporary views on the pathogenesis of hydroneophrosis]. | 2016 | 4862153 | |
| massachusetts metabolic disorders screening program. i. technics and results of urine screening. | 2016 | 5041315 | |
| attenuation characteristics of recreational helmets. | 2016 | 4811574 | |
| a new disposable electrode model for clinical routine fhr monitoring. | 2016 | 4806564 | |
| sprained ankles. iv. histologic changes in recent and "chronic" ligament ruptures. | 2016 | 4959236 | |
| development of cellular dependence on infective organisms: micrurgical studies in amoebas. | nuclei and cytoplasm were transferred between a normal strain anda variant strain of amoeba discoides heavily infected with bacteria. after 5 years of infection, the infective bacteria that were initially harmful to the host cells became harmless, and the nucleus of the host cell became dependent on the infective organisms for its normal functions. | 2016 | 5035468 |
| [tablets. 8. study of excipients for direct compression pure, and in presence of phenobarbital, on reciprocating machine]. | 2016 | 4771727 | |
| blunt trauma to abdomen and chest. (review of a 7-year experience and report of 3 interesting cases in childhood). | 2016 | 4846087 | |
| a factorial study of existential state reactions. | 2016 | 4766707 | |
| dentist's libel action continues. | 2016 | 5040723 | |
| studies on muscle rigidity: droperidol, diazepam, and promethazine. | 2016 | 5062116 | |
| [review notes on toxoplasmosis]. | 2016 | 4987998 | |
| [brill-symmers giant follicular lymphoma localized in pelvic lymph nodes]. | 2016 | 4882834 | |
| supraclavicular catheterization of the superior vena cava. | 2016 | 4836144 | |
| theory of competitive binding of iron by different transferrins. | 2016 | 4824920 | |
| [total invagination of the appendix, simulating a cecal neoplasm]. | 2016 | 4714895 | |
| [active geriatrics. a visit to rotterdam]. | 2016 | 4706650 | |
| [occupational medicine problems of an aviation physician]. | 2015 | 4701759 | |
| [occupational medicine problems of an aviation physician]. | 2015 | 4701759 | |
| an enzymatic determination of ammonia in biological fluids. | 2015 | 4284306 | |
| [preleukemic state with symptoms of aplastic anemia]. | 2015 | 4674957 |