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observations on erythrocytic acetylcholinesterase activity in experimentally induced anaplasmosis in calves.erythrocytic acetylcholinesterase (ache) activity was assessed in splenectomized bovine calves inoculated with anaplasma marginale. other parameters, i.e., haematological values (haemoglobin, packed-cell volume, and total erythrocytic count), percentage parasitaemia and the ensuing host reactions during anaplasma infection were also studied in relation to ache activity. there was significant inhibition of red blood cell-ache activity commencing soon after inoculation of infected blood; this prec ...19853992879
cross-reaction of tick salivary antigens in the boophilus microplus-cattle system.calves were immunized with boophilus microplus saliva, filtered through millipore membranes, in freund's complete adjuvant. serum samples were tested by passive hemagglutination against babesia bigemina, anaplasma marginale, b. microplus larvae extract, stomoxys calcitrans extract and b. microplus saliva. after immunization, titers to saliva, larval tick-extract and to s. calcitrans were increased. the challenge with live tick larvae enhanced the formation of antibodies against larva extract, fl ...19853992881
anaplasma marginale infections in american bison: experimental infection and serologic study.anaplasma marginale was experimentally transmitted from cattle to bison and back to cattle. of the 2 splenectomized and 1 intact american bison calves (bison bison) inoculated with a north texas a marginale stabilate, 1 splenectomized and 1 intact bison exhibited clinical signs of anaplasmosis. active parasitemias in these bison were observed along with positive reactions in the rapid card agglutination and complement fixation tests. blood from the infected bison produced disease in splenectomiz ...19853994109
demonstration of vaccine-induced immunity to anaplasmosis without induction of persistent postvaccinal complement-fixing and agglutinating antibodies in yearling steers.the protective effect and anti-anaplasma complement-fixing and agglutinating antibody responses induced in yearling steers by vaccination with an inactivated anaplasma marginale vaccine were evaluated by challenge exposure. eleven 12- to 14-month-old hereford x angus steers were randomly allotted into 6 principals and 5 controls. the principals were injected im with 5 ml of vaccine on days 0 and 21. on day 70, the 11 steers were challenge exposed by iv inoculation of a marginale-infected blood. ...19853994127
evaluation of jackrabbits as nonruminant hosts for anaplasma marginale.two black-tailed jackrabbits (lepus californicus), 1 splenectomized and 1 intact, were inoculated with 0.2 ml of a 1:5 dilution of a florida anaplasma marginale stabilate. five months later, both hares were inoculated with 1 ml of whole blood from a calf with acute anaplasmosis. neither hare developed any signs of clinical anaplasmosis. pooled blood (7 ml) from these jackrabbits which was inoculated into 2 anaplasma-susceptible, splenectomized calves failed to induce hematologic or serologic sig ...19853994131
peritubular protein modulates neutral active nacl absorption in rabbit proximal convoluted tubule.to assess directly the effect of increasing and decreasing peritubular protein concentration on active nacl absorption, rabbit proximal convoluted tubules were perfused with a high-chloride solution simulating late proximal tubular fluid and bathed in a similar solution containing 0, 6, or 10 g/dl albumin. a direct examination of the active component of nacl absorption was ensured by 1) the reduction of luminal solutes other than nacl (no glucose or alanine and only 5 mm hco-3), and 2) the use o ...19854003554
hexaprenylpyrophosphate synthetase of micrococcus luteus b-p 26. 19854021814
hexaprenylpyrophosphate synthetase of micrococcus luteus b-p 26. 19854021814
transmission of anaplasma marginale by adult dermacentor andersoni during feeding on calves.laboratory-reared dermacentor andersoni ticks experimentally infected as nymphs with anaplasma marginale were allowed to feed as adults from 1 to 9 days on susceptible, splenectomized calves to determine when, during feeding, the hematozoan was transmitted from ticks to cattle. in experiment 1, ticks were allowed to feed on calves for 1, 2, 3, 4, 5, or 6 days and anaplasmosis did not result. the same calves were used for experiment 2, and ticks were allowed to feed for 1, 3, 6, 7, 8, or 9 days a ...19854026042
comparison of the cytotoxic activities of chemotherapeutic drugs using a human bladder cancer cell line.many chemotherapeutic drugs have been used to treat patients with advanced bladder cancer, but few of these have been evaluated adequately in phase ii clinical trials. continuous cell lines provide one means for comparing the in vitro cytotoxicities of anticancer agents. in this study, a continuous cell line derived from a transitional cell cancer of the human bladder, which still produces tumours histologically similar to the tumour of origin on xenotransplantation, was used to measure the in v ...19854039488
direct observations of responses of mesenteric microcirculation of the rat to circulating noradrenaline.the responses of mesenteric microcirculation of the rat to circulating noradrenaline were studied by in vivo microscopy, using a photo-electric device placed on a television monitor to measure changes in diameter of individual vessels. blood flow was measured in the anterior mesenteric artery with an electromagnetic flow transducer, mesenteric vascular conductance was computed on-line from mesenteric artery flow and arterial pressure. i.v. injection of noradrenaline induced a decrease in diamete ...19854078744
[interaction of local anesthetics with secretory cells at the membrane level]. 19852408715
presence of common antigens, including major surface protein epitopes, between the cattle (intraerythrocytic) and tick stages of anaplasma marginale.epitopes of major surface proteins of the intraerythrocytic cattle stage of anaplasma marginale were demonstrated in the midgut stage of the organism within the infective tick host dermacentor andersoni. these proteins were common to all a. marginale isolates tested and at all stages of parasitemia. sera from cattle immunized with the tick midgut stage of a. marginale immunoprecipitated multiple-erythrocyte-stage proteins, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophore ...19852415457
[ecto-atpases in leukocytes and its modification by drugs].the ecto-atpase is located on the external surface of the plasma membrane of intact cells. the enzyme splits exogeneous atp in the presence of mg2+ into adp and inorganic phosphate. this enzyme has been found on a variety of cells of various species in different activities. in leukocytes sulfhydryl groups are essential for the structure and function of the ecto-atpase. the physiological and biochemical significance of the ecto-atpase for cells remains unclear. drugs may influence ecto-atpase act ...19852859769
prevalence of cytomegalovirus antibodies in thai blood donors.antibodies to cytomegalovirus (cmv) were determined in thai blood donors using the complement fixation (cf) test and enzyme-linked immunosorbent assay (elisa). a total of 203 voluntary blood donors, 181 males and 22 females, who came to the blood bank at siriraj hospital during february 1985, were investigated. their ages ranged from 17 to 53 years (mean 24.3 +/- 6.9). seventy-three out of 156 (46.8%) and 171 out of 203 (84.2%) sera were positive for cmv antibodies as detected by the cf test and ...19853000401
blood volume regulation. on the right lines at last? 19853157061
bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase.in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ...19863005034
bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase.in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ...19863005034
long-term culture of human granulocytes and granulocyte progenitor cells. 19863008194
further evidence that a beta-adrenergic mechanism regulates water intake: role of the subfornical organ.the daily water intake was reduced in rats by subcutaneous administration of propranolol. the fluid intake after 24 h of dehydration in nephrectomized animals was decreased by propranolol. in intact rats diazepam did not modify the postdehydration water intake. propranolol injected into subfornical organ decreased water consumption after 24 h of fluid deprivation but did not change the dipsogenic response to carbachol given in the same structure. these results suggest that a beta-adrenoceptor me ...19863019711
further evidence that a beta-adrenergic mechanism regulates water intake: role of the subfornical organ.the daily water intake was reduced in rats by subcutaneous administration of propranolol. the fluid intake after 24 h of dehydration in nephrectomized animals was decreased by propranolol. in intact rats diazepam did not modify the postdehydration water intake. propranolol injected into subfornical organ decreased water consumption after 24 h of fluid deprivation but did not change the dipsogenic response to carbachol given in the same structure. these results suggest that a beta-adrenoceptor me ...19863019711
cloning and characterization of the high-affinity camp phosphodiesterase of saccharomyces cerevisiae.a gene, pde2, has been cloned from the yeast saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of ras2val19, a mutant form of the ras2 gene that renders yeast cells sensitive to heat shock and starvation. it has previously been shown that the ras proteins are potent activators of yeast adenylate cyclase. we report here that pde2 encodes a high-affinity camp phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. these results t ...19863025832
activity of ciprofloxacin against resistant clinical isolates.the in-vitro activity of ciprofloxacin, a new quinolone derivative, was determined by broth microdilution for 518 clinical isolates. the mics for 90% of the organisms were 2 mg/l for pseudomonas aeruginosa, 8 mg/l for p. maltophilia, 4 mg/l for other pseudomonas spp., 1 mg/l for acinetobacter anitratus, 0.03-0.5 mg/l for differing species of enterobacteriaceae, 2 mg/l for streptococcus faecalis, 0.25 mg/l for coagulase-negative staphylococcus spp. and 2 mg/l for s. aureus. furthermore, on a weig ...19862939048
[involvement of arterial vessels in the blood supply to adenomas of the human pituitary, functional implications].among 190 human pituitary adenomas investigated, 140 contained arterial blood vessels (nearly 75 percent). in 160 adenomas (prl-, gh-secreting or nonfunctioning tumors) arterial vessels were present in 80 to 90 percent of the cases, whereas in 30 corticotropic adenomas (cushing's disease), 5 only contained arterial vessels. according to data of the literature and to our own findings, such vessels should originate from the anterior pituitary itself or from the surrounding structures (trabecular, ...19862832021
[immunological studies in hamsters (cricetus auratus) infected with schistosoma mansoni]. 19863105038
hepatitis d in new zealand preliminary survey. 19863459096
sympathetic, muscarinic vasodilation in cranial vessels of the cat.pressor responses evoked by stimulation of the preganglionic sympathetic trunk of the feline superior cervical ganglion have been recorded in vivo from the vascular bed perfused by one external carotid and the vertebral artery. when vasoconstrictor activity is blocked and potential vasodilator activity enhanced by close, intracarotid injection of guanethidine and prostaglandin f2 alpha respectively, stimulation evokes a weak pressor response followed, on cessation of stimulation, by a prolonged ...19863460813
renal cell carcinoma in the wistar-lewis rat: a model for studying the mechanisms of cholesterol acquisition by a tumor in vivo.renal adenocarcinoma implanted into isogeneic wistar-lewis rats closely resembles human renal cancer. this paper characterizes the tumor's growth rate, metastatic potential, and its light and electron microscopic appearance. additionally, for the first time, the pathways through which a tumor acquires the cholesterol needed for growth were quantified in vivo. two 1-mg pieces of renal carcinoma were implanted beneath the renal capsule of 80 wistar-lewis rats. of the implanted tumors 95% "took" an ...19863486039
deficient expression of mhc class ii antigens in some cases of human b cell leukaemia.cells from the peripheral blood of patients with b cell chronic lymphocytic leukaemia (b-cll) and acute lymphoblastic leukaemia (all) were examined for the expression of mhc class ii antigens, using a number of monoclonal antibodies (moab) including l243 (anti-dr) and tu22 (anti-dq). there was wide variation in expression of mhc class ii antigens in cll, both from patient to patient and among cells from the same individual. in a number of subjects a significant proportion of the cells had detect ...19863486060
anaplasma marginale, eperythrozoon wenyoni: lectin reactions with bovine erythrocytes.normal bovine erythrocytes were agglutinated with four of five lectins specific for different oligosaccharides. the order of reactivity was wheat germ greater than ricin greater than soybean greater than peanut. concanavalin a did not agglutinate normal bovine erythrocytes. after neuraminidase treatment of normal bovine erythrocytes, each lectin agglutinated the cells with decreased concentrations of lectin, verifying that partial removal of sialic acid exposes more of each lectin's binding site ...19863943586
immunization with an isolate-common surface protein protects cattle against anaplasmosis.hemoparasitic diseases are endemic in half the world's livestock production areas and are the greatest obstacle to improved meat, milk, and fiber production in the third world. the most prevalent of these diseases, anaplasmosis, occurs throughout tropical and subtropical regions and is responsible for 50,000 to 100,000 cattle deaths annually in the united states alone. despite its prevalence and the severity of the losses, an effective immunoprophylaxis for anaplasmosis has not been developed. a ...19863945825
isolate of anaplasma marginale not transmitted by ticks.the tick-borne transmissibility of 2 isolates of anaplasma marginale was compared. dermacentor variabilis were exposed to a marginale as nymphs by feeding on 1 of 4 splenectomized calves during periods of ascending parasitemia (maximum 49% to 81% parasitized erythrocytes) induced by injection of a stabilate. tick-borne transmission was attempted, using 26 to 224 adult ticks within 30 to 220 days after molting. adult d variabilis did not transmit an illinois isolate of a marginale in 7 tick-borne ...19863946891
c-rash and ornithine decarboxylase are induced by oestradiol-17 beta in the mouse uterine luminal epithelium independently of the proliferative status of the cell.oestradiol-17 beta (e2) treatment of the ovariectomized mouse results in a synchronised wave of cell proliferation in the uterine luminal epithelium. at the peak of dna synthesis the mrna level of the c-rash proto-oncogene and ornithine decarboxylase were significantly increased. progesterone treatment completely inhibits the e2 induced wave of dna synthesis but does not greatly influence the level of these 2 mrnas. thus in the uterine luminal epithelium e2 regulates the level of ornithine decar ...19863949003
observations on histamine, serotonin, cortisol and cortisol binding globulins in bovine anaplasmosis.changes in histamine, serotonin, cortisol and cortisol binding globulin (cbg) levels were observed in anaplasma marginale-infected bovine calves. in addition, packed cell volume (pcv), platelet number and percentage parasitaemia were also recorded. the whole blood histamine and serotonin values rose significantly (p less than 0.01 and p less than 0.025, respectively) during the acute stage of anaplasma infection. higher serum cortisol and cbg levels (p less than 0.05) were observed in acute and ...19863962160
identification of antigens of two isolates of anaplasma marginale, using a western blot technique.antigens of the illinois (iam) and florida (fam) isolates of anaplasma marginale were analyzed, using the western blot technique and antiserum from a marginale-infected calves. crude antigens were prepared from the parasitemic blood of each. antiserum was collected after the primary and recrudescent parasitemias. antigens were separated, using sodium dodecyl sulfate polyacrylamide gel electrophoresis. antigens were then transferred onto nitrocellulose membranes and exposed to test sera. antibodi ...19863963551
epidemiologic aspects of bovine anaplasmosis in semiarid range conditions of south central idaho.the prevalance of anaplasma marginale-infected cows, as determined by use of the modified rapid card agglutination (mrca) test, was measured during a 4-year period (1980-1983). the prevalence of a marginale-infected cows, defined as positive reactors on the mrca test, remained constant (31%-37%). the apparent incidence of a marginale transmission to susceptible cows was approximately 7% from 1980 to 1981, 8% from 1981 to 1982, and no transmission from 1982 to 1983. the occasional mrca-positive c ...19863963555
kinetic analysis of lamb mutants suggests the signal sequence plays multiple roles in protein export.we have developed a quantitative assay to measure the rate of processing of precursor lamb into mature protein and have used this assay to characterize 10 previously isolated and 3 new lamb signal sequence mutants. the data suggest that the lamb signal sequence serves a complex function. our assay has revealed five types of signal sequence defect: 1) a strong kinetic defect resulting from alteration of the secondary structure in the putative alpha-helical region in the hydrophobic core, 2) a str ...19863533933
biosynthetic preparation of radioactively labelled ethanolamine plasmalogen (1-o-[1'-14c]octadec-1'-enyl-2-acyl-sn-glycero-3-phosphoethanola mine) using a protozoan cell culture.ethanolamine plasmalogen radiolabelled mainly in the o-alkenyl moiety was prepared from cell suspension cultures of the flagellate leishmania donovani previously incubated with [1-14c]octadecanol over one growth period. the optimal concentration of [1-14c]octadecanol for labelling was shown to be 1 microm, when 60% of total lipid radioactivity appeared in the 1,2-diradyl-sn-glycero-3-phosphoethanolamine fraction, with an overall yield of approx. 35%. analysis of this fraction revealed that 93% o ...19863536139
prevalence of anaplasma marginale, babesia bigemina and b. bovis in nigerian cattle using serological methods.serum samples collected randomly from 500 cattle from the 10 northern states of nigeria were tested for antibodies against anaplasma marginale by indirect fluorescent antibody (ifa), card agglutination (ct) and capillary tube-agglutination (ca) tests. the serum samples were also examined for antibodies to babesia bigemina and b. bovis by the ifa test only. of the serum samples tested, 79.4% had antibodies against a. marginale by the ifa test, 40 and 25% in the ct and ca tests, respectively. the ...19863538640
origin of ascending auditory projections to the nucleus mesencephalicus lateralis pars dorsalis in the chicken.ascending auditory projections to the nucleus mesencephalicus lateralis pars dorsalis (mld) were studied in white leghorn chickens by means of unilateral injections of horseradish peroxidase into the mld and by injections of tritiated leucine into nucleus angularis or the combined nucleus magnocellularis and nucleus laminaris. the experiments showed that nucleus angularis sends an extensive projection to the contralateral mld and a smaller projection to the rostral pole of the ipsilateral mld; t ...19863697720
field observations on adult zebu bulls immunised against anaplasmosis by infection and treatment plus integrated tick control.fourteen adult zebu bulls which were negative for anaplasma marginale infection both serologically and on blood smear examinations were infected with a virulent nigerian isolate of a. marginale. forty days following the immunising infection when clinical reactions were established groups of seven animals were treated with either two doses of imidocarb dipropionate (2 x 5 mg/kg given intramuscularly 14 days apart) or a single intramuscular dose of long-acting oxytetracycline (5 mg/kg). following ...19863705174
an effective economical intradermal regimen of human diploid cell rabies vaccination for post-exposure treatment.a closely-spaced multisite intradermal regimen of human diploid cell rabies vaccine (hdcv) was evaluated in 39 patients after low-risk exposure to rabies, in comparison to full-dose intramuscular hdcv and sheep brain-derived rabies (semple) vaccine. the regimen consisted of four intradermal injections, 0.1 ml each of hdcv on days 0, 3 and 7, followed by two booster doses of only 0.1 ml each on days 28 and 91 administered intradermally. although the total amount of hdcv used in this intradermal r ...19863708902
spherocytosis associated with anaplasmosis in two cows.spherocytes were detected on blood smears of 2 angus cows. the rbc from both cows had increased fragility in hypotonic saline solution, supporting the presence of spherocytes. other laboratory tests revealed a macrocytic, regenerative anemia with anisocytosis, polychromasia, basophilic stippling, howell-jolly bodies, nucleated rbc, and anaplasma marginale organisms. a complement-fixation test was positive for anaplasmosis in cow 1 and a direct coombs' test was positive for immunoglobulin g in bo ...19863710896
detection of an anaplasma marginale common surface protein present in all stages of infection.the establishment and maintenance of anaplasmosis-free cattle herds is impaired due to the lack of a rapid, sensitive, and specific serologic test to detect persistently infected cattle which serve as carriers for the organism. to develop an improved diagnostic test for anaplasmosis we screened anaplasma marginale initial body proteins to identify a protein common to antigenically different isolates that is recognized by the host immune system at all stages of infection. seronegative cattle were ...19863711298
experimental anaplasmosis in american bison: persistence of infections of anaplasma marginale and non-susceptibility to a. ovis.blood collected 314 and 496 days after experimentally infecting splenectomized and spleen-intact american bison (bison bison) with anaplasma marginale was infective for splenectomized bovine steers. the pathogenesis was identical to that seen in bovine studies using bovine blood inoculations. a splenectomized bison remained normal clinically, hematologically and serologically for 10 mo after repeated inoculation of ovine blood infected with a. ovis.19863712642
biological activities of monoclonal antibodies to the hemagglutinin-neuraminidase (hn) protein of newcastle disease virus. 19863712896
enhanced o6-methylguanine-dna alkyltransferase activity in rat liver nodules.the progression of chemical carcinogen induced hepatic lesions in the rat from altered enzyme foci to hepatocyte nodules and ultimately to hepatocellular carcinoma appears to be related to the evolution of new cell populations within these hepatic lesions. initiator-promoter-initiator experiments in rat liver models using alkylating agents as the second genotoxic compound suggest accelerated progression toward malignancy could be the result of mutations caused by o6-alkylguanine formation in the ...19863719565
a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum.an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ...19863729834
dynamics of anaplasma marginale in splenectomised calves treated with either imidocarb or oxytetracycline.neither imidocarb dipropionate (at 5 mg/kg body weight intramuscularly once or twice at a seven-day interval) nor oxytetracycline hydrochloride (at 20 mg/kg body weight intramuscularly once or twice at a seven-day interval) given to animals in the prepatent period of anaplasmosis, prevented the eventual onset of infection. both compounds delayed the onset of detectable parasitaemia but this action was more noticeable with oxytetracycline. oxytetracycline given twice significantly reduced the sev ...19863739000
longevity of colonies of anaplasma marginale in midgut epithelial cells of dermacentor andersoni.colonies of anaplasma marginale in midgut epithelial cells of experimentally infected dermacentor andersoni were studied in adult ticks 1, 3, and 6 months old. longevity of the parasite in ticks was assessed by evaluating its infectivity for splenectomized calves; calves were exposed by feeding ticks and by inoculation of tick gut homogenates. longevity was also evaluated by determining size, type, and density of colonies in male and female ticks. the effect of incubation (2.5 days at 37 c) on c ...19863752671
percutaneous infection of nymphal dermacentor andersoni with anaplasma marginale.newly replete nymphal dermacentor andersoni (principals) were percutaneously exposed to anaplasma marginale by injection of either intact or lysed infected bovine erythrocytes. control nymphs were fed on calves with anaplasmosis. the subsequently molted adults were examined for infection by light microscopy, and companion ticks were tested for infectivity by allowing them to feed on susceptible calves. when they fed as adults, both control ticks and percutaneously inoculated principals transmitt ...19863752672
[study of alveolar macrophages stimulated with zymosan in vivo using fluorescent probes].zymosan granules injected intravenously (100 mg per kg of body weight) to wistar rats induced strong stimulation of mononuclear phagocyte system. on day 5 zymosan-induced recruitment of monocyte-like cells with high consuming and bactericidal activity into lung spaces could be observed. using fluorescent probes, it was discovered that the size of alveolar cell membranes in zymosan-stimulated or non-stimulated animals was similar, while the viscosity of membrane lipids of alveolar macrophages was ...19863756335
a comparison of the effects of climbing fiber deafferentation in adult and weanling rats.the climbing fiber input to the cerebellar cortex was destroyed using both electrolytic and chemical (3-acetylpyridine) lesions. the long-term effects of climbing fiber deafferentation on the ansiform lobule of weanling and adult rats were examined at both the light and electron microscopic levels. image analysis of golgi-impregnated purkinje cells indicated a significantly lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of both adult and weanling ra ...19863768690
transmission of anaplasma marginale theiler by males of dermacentor andersoni stiles fed on an idaho field-infected, chronic carrier cow.the role of ticks and carrier cattle in epizootics of bovine anaplasmosis was further clarified by demonstrating unequivocally, for the first time, that male ticks fed on a chronic carrier cow naturally infected with anaplasma marginale can transmit this parasite intrastadially and biologically when subsequently fed on susceptible cattle. these data indicate that field epizootics of acute anaplasmosis may be initiated by males of tick vector species that feed on carrier cattle and subsequently t ...19863777655
hydatid disease: research and control in turkana. ii. the role of immunological techniques for the diagnosis of hydatid disease.immunological studies have been applied to the immunodiagnosis of human hydatidosis in turkana and for the specific identification of echinococcus eggs, particularly in regard to their potential for the assessment of a hydatid control programme in the north-west of the district. a high rate of false negatives has been obtained with sensitive antibody tests for proven hydatid patients in turkana and presents problems for prevalence estimation in the population. a seroepidemiological study using s ...19863787677
rem-sleep deprivation-induced increase in ethanol intake: role of brain monoaminergic neurons.the ethanol intake of long-evans male rats was recorded before, during and after deprivation of rapid eye movement (rem) sleep produced with the flowerpot technique modified by using a cuff pedestal and electrified grid floor instead of water. ethanol intake increased significantly during rem-sleep deprivation. a rebound decrease in ethanol drinking was then observed during the rem-rebound phase immediately after the termination of rem-sleep deprivation. because rem-sleep deprivation has been re ...19863814349
[morphofunctional changes in the adrenergic innervation of the major cerebral arteries during bilateral electrostimulation of the locus coeruleus].the intramural adrenergic nervous apparatus of cerebral arteries was studied in adult rabbits after 3-10 sessions of electrical stimulation of locus coeruleus. the activity of nerve structures was determined by estimating the density of adrenergic perivascular plexuses and by semi-quantitative cytophotometry of changes in the catecholamine content of nerve varicosities. the stimulation was followed by a 28.2 +/- 1.5% increase in adrenergic innervation density. while catecholamine content in peri ...19873814808
bovine anaplasmosis: susceptibility of seronegative cows from an infected herd to experimental infection with anaplasma marginale.adult cows from an anaplasma marginale-infected herd that were negative to the a marginale rapid card agglutination (rca) and complement fixation (cf) tests for 1 to 4 years developed acute anaplasmosis after inoculation with 0.5 ml of blood from an a marginale carrier cow. the test cattle were as susceptible as the control cattle of similar ages. also, 2 cows that had seroconverted from rca/cf-positive to rca/cf-negative status naturally were fully susceptible to anaplasmosis when they were exp ...19873818429
infectivity of three anaplasma marginale isolates for dermacentor andersoni.three isolates of anaplasma marginale--virginia (vam), illinois (iam), and florida (fam)--were compared for infectivity for dermacentor andersoni. the isolates were selected, in part, because of a tail-like appendage that has been demonstrated in the vam and iam, but not in the fam. ticks were exposed to the isolates as nymphs either naturally by feeding on a calf with anaplasmosis or artificially by percutaneous inoculation with infected bovine erythrocytes. they were examined for infectivity a ...19873826850
immunocytochemical localization of neuropeptide y-like immunoreactivity in adrenergic and non-adrenergic neurons of the rat gastrointestinal tract.the immunocytochemical location of neuropeptide y (npy)-like immunoreactivity (li) within the neuronal structures of the rat gastrointestinal (gi) tract was investigated with the indirect immunofluorescence method. npy immunoreactive neurons were found throughout all regions of the gi tract with the largest number in the duodenum. npy immunoreactive perikarya were mainly located in the submucosal ganglia. npy labeled processes were extensively seen in the submucosal and myenteric plexuses, smoot ...19873554165
neuropeptide y and its flanking peptide in human endocrine tumors and plasma.neuropeptide y (npy) and its flanking peptide (cpon) were measured in extracts of pheochromocytomas (n = 26), ganglioneuroblastomas (n = 8), and other tumors (n = 95) and plasma (n = 38). npy was present in high concentrations in the majority of the pheochromocytomas, but was absent in 3 tumors. similar concentrations of npy were measured using n- and c-terminal-directed antisera, and there was a good correlation between the concentrations of npy immunoreactivity and cpon immunoreactivity in the ...19873571423
detection of antibodies to anaplasma marginale by an improved enzyme-linked immunosorbent assay with sodium dodecyl sulfate-disrupted antigen.sensitivities of two enzyme-linked immunosorbent assays (elisas) with particulate and sodium dodecyl sulfate (sds)-disrupted anaplasma marginale antigen were compared. the quotient of positive reference sera divided by the absorbance quotient of a negative reference serum at identical dilution was termed the signal-to-noise ratio. optimal signal-to-noise ratios were dependent on both pretreatment of antigen and antigen concentration. sds disruption of anaplasmal antigen resulted in a markedly im ...19873571472
influence of imidocarb prophylaxis on the pathogenicity and immunogenicity of anaplasma marginale infections in cattle. 19873579755
the genome of anaplasma: dna base composition and dna/dna hybridization.the tm value of dna from anaplasma centrale and anaplasma marginale was found to be 87.1 degrees c and 89.3 degrees c, respectively. the g + c content, calculated from the tm, was 45.1% for a. centrale and 48.5% for a. marginale. identical hybridization patterns were obtained when the dna from one species was hybridized to restriction endonuclease-digested dna from the other species.19873587929
persistence of colonies of anaplasma marginale in overwintering dermacentor variabilis.dermacentor variabilis were infected as nymphs with anaplasma marginale by allowing the ticks to feed on a single infected donor calf. two weeks after molting to the adult stage, the ticks were allotted into 1 of 3 groups and were allowed to overwinter at room temperature (25 c) in the laboratory (group 1), cold storage (4.5 c) in the laboratory (group 2), or outdoors in leaf litter (group 3). persistence of a marginale was assessed by determining density of colonies (number of colonies/0.1 mm2 ...19873592362
pathology of brain parenchyma in meningeal carcinomatosis; immunohistochemical study with astroprotein (gfap) and tubulin.effects of leptomeningeal tumor on the brain parenchyma was studied by the immunohistochemical method with astroprotein (gfap) and tubulin in a rat model of meningeal carcinomatosis. thickening of subpial glial lining (external glial layer) and hypertrophy of subpial astrocytes, detected by the antiserum to gfap, was the early sign of parenchymal involvement. the glial lining was continuous as far as the tumor cells were confined to the subarachnoid space, however, penetration of tumor cells int ...19873598623
tick infestations on livestock in the yemen arab republic and their potential as vectors of livestock diseases.a survey of ticks on cattle, camels, sheep, goats and donkeys in four different geographical locations of the yemen arab republic (yar) was carried out to provide more information on the possible risk of tick-borne diseases to imported exotic cattle included in the yar's plans for livestock development. the most abundant ticks were hyalomma spp. particularly on camels. ticks found on cattle included hyalomma spp., amblyomma variegatum, boophilus annulatus and rhipicephalus spp. in general with t ...19873603710
vaccination with anaplasma centrale: response after an experimental challenge with anaplasma marginale.the haematological and clinical responses to vaccination with anaplasma centrale and to subsequent challenge with anaplasma marginale were evaluated. twenty holstein steers 14 to 16 months of age were divided into two groups of 12 and eight animals respectively (groups i and ii). group i was inoculated on day zero with 10(7) a. centrale-infected erythrocytes and group ii was kept as a control. on day 125 both groups were challenged with 5 x 10(7) a. marginale-infected erythrocytes. a. centrale i ...19873629722
attempted transmission to cattle of anaplasma marginale from overwintered dermacentor andersoni ticks.since the 1983 summer outbreak of anaplasmosis in southern saskatchewan, the role of the tick, dermacentor andersoni as an overwintering reservoir for anaplasma marginale has been questioned. the purpose of this study was to determine if spring-collected ticks carried virulent a. marginale. sixteen splenectomized calves were assigned randomly to two groups of 14 principals and two controls. adult d. andersoni, collected in april from areas having high transmission rates of a. marginale, were con ...19873651893
[the effect of trapidil and the trapidil derivative ar 12463 on blood pressure and thromboxane formation in spontaneously hypertensive rats].the trapidil derivative ar 12463 has a much more stronger inhibiting effect than trapidil on different atherosclerotic parameters of the cell cultures obtained from normal intima and fatty streaks of human aorta, on thromboxane (tx) formation and on the aggregation of human platelets in vitro, and on the activity of phosphodiesterase. in addition to these present results, ar 12463 reduced significantly the mean arterial blood pressure of spontaneously hypertensive rats (at a dosage of 20 mg/kg/d ...19873671447
synthesis of thiazole derivatives with positive inotropic effect.thiazole derivatives with potential positive inotropic effect were synthesized. the highest activity (medium positive inotropic effect) was exhibited by ethyl 2-(2-hydroxyethylamino)-4-phenylthiazole-5-carboxylate (3b) of which several derivatives have been prepared. among these derivatives only those which could presumably easily removed in the living organism to give 3b exhibited a medium positive inotropic effect (6, 8b). it was assumed that the effect of 3b had a beta-agonist character.19873671455
withdrawal of zinc oxide-containing diets from mature, female domestic fowl: effects on selected tissue mineral contents.1. two hundred and sixteen laying hens of two strains were given diets containing zno to provide 0, 0.5, 0.75, 1.00, 1.25, 1.50, 2.00, 3.00 or 4.00 g added zn/kg. these diets were offered ad libitum for 5 consecutive 28-d periods. all hens were subsequently offered the control diet with no added zno for 5 weeks. 2. four hens from each treatment were killed 0, 1 and 5 weeks after substitution of the zno-supplemented diets by the control diet. 3. dietary addition of zno significantly increased liv ...19873676878
endorectal pull-through with conservation of the rectal mucosa: experimental study.the present experimental work was designed to study an abdominoanal pull-through technique, with conservation of rectal mucosa. continence was evaluated from clinical, radiologic, and histopathologic standpoints. the animals defecated normally. a good retention of barium was seen in the radiologic enema. on the reexploration of the animals, we found a distal dehiscence with elevation of the intussuscepted stump towards the rectum in all. a reepithelization process of serosa of the intussuscepted ...19873681633
different effects of reinforcing and reducing manipulations in acupuncture assessed by tail-flick latency, vocalization threshold and skin temperature in the rat. 19873497315
an enzyme linked immunosorbent assay to diagnose babesia bovis infection in cattle.the optimum gel filtration fraction from lysate of babesia bovis infected erythrocytes was determined for use as an antigen in an elisa to diagnose b. bovis infection in cattle. of four enzyme labels tested, horseradish peroxidase was the most suitable. the assay is both sensitive and specific in detecting antibody for 2-4 years after a single infection. false positive reactions were obtained only with some sera from some anaplasma marginale infected cattle.19873106957
influence of castration and testosterone propionate on prostatic and seminal vesicular lipids in mature monkeys.the effect of castration and administration of testosterone propionate (tp) has been studied in mature monkeys in relation to prostatic and seminal vesicular lipids to castrates on prostatic and seminal vesicular lipids were studied in mature monkeys. castration decreased prostatic total lipid, total phospholipids and total glyceride glycerols. among neutral lipid classes only diacyl glycerol was decreased due to castration. similarly among phospholipid classes, phosphatidyl choline and ethanola ...19873429027
the persistence of colostral anaplasma antibodies and incidence of in utero transmission of anaplasma infections in calves under laboratory conditions.twenty-six calves, born from 25 anaplasma-infected, intact and splenectomized cows, from a herd kept under strict tick-free laboratory conditions, were monitored for the presence of anaplasma antibodies, using the rapid card agglutination test. serum was collected at birth, weekly for 12 weeks, and then monthly for approximately 6 months. specific antibodies passively acquired could be detected in calf sera for an average period of 8 weeks after birth. calves that remained positive for longer th ...19873444609
interaction of magnesium ion, oxygen tension, and temperature in the production of toxic-shock-syndrome toxin-1 by staphylococcus aureus. 19873102633
dna probes for the detection of anaplasma centrale and anaplasma marginale.anaplasmosis can be diagnosed either by immunological techniques or by direct microscopic examination of blood smears. both methods are time-consuming and labour intensive. the use of dna probes in an hybridization assay may simplify the diagnosis of anaplasmosis in cattle and sheep. a genomic dna library of anaplasma centrale was constructed in an expression vector and screened to detect clones containing a. centrale dna. four probes which hybridized to a. centrale and anaplasma marginale dna w ...19872832800
genetic analysis of human b cell hybridomas expressing a cross-reactive idiotype.we have examined the genetic basis for the expression of a human cross-reactive idiotype (cri) commonly found on monoclonal igm rheumatoid factors. the cri was identified with a monoclonal antibody (17.109) and has been localized previously to the kappa-variable region. by using the human lymphoblastoid cell line wi-l2-729-hf2, and mononuclear cells from several sources, a panel of hybridomas was generated that produced 17.109 cri-positive ig. a recently cloned human germ-line v kappa iii gene, ...19872433336
influence of gangliosides on primary and metastatic neoplastic growth in human and murine cells.the influence of gangliosides on tumor growth and frequency of metastasis in vivo as well as on growth and motility of neoplastic cells in vitro was tested utilizing human and rodent cell populations. in mice receiving injections of a ganglioside mixture twice daily the tumor volume, the number of spontaneous metastases per animal, and the number of mice with metastasis was approximately double that of controls. preincubation of neoplastic cells with the ganglioside mixture doubled the number of ...19872440560
characterization of an immunoprotective protein complex of anaplasma marginale by cloning and expression of the gene coding for polypeptide am105l.immunization with an anaplasma marginale surface protein complex containing two polypeptides (am105u and am105l), each having a molecular weight of 105,000, protected cattle against challenge with virulent organisms. these polypeptides were immunoprecipitated together from detergent extracts of a. marginale by a neutralizing monoclonal antibody. after surface radioiodination of intact parasites, both am105u and am105l contained the radiolabel. to define the structural and antigenic relationships ...19872443451
immunological studies in haemophilic children.a majority of haemophiliacs who have received large-pool plasma products within the past 5 years have been exposed to the putative agent of the acquired immunodeficiency syndrome (aids)--hiv. it is not known what the risk of infection is among patients in south africa. a study was made of 39 children with congenital coagulation disorders attending the red cross war memorial children's hospital haemophilia clinic. all but 3 had been treated exclusively with small-pool lyophilised cryoprecipitate ...19872953077
in vivo pharmacology of ici 198,615: a novel, potent and selective peptide leukotriene antagonist.the in vivo pharmacology of ici 198,615, a highly potent and selective peptide leukotriene (lt) receptor antagonist, is described. in a conscious guinea pig model, ici 198,615 provided dose-related antagonism of aerosol ltd4-induced dyspnea when administered p.o., i.v. or by aerosol. the pharmacologic half-lives of ici 198,615 by these routes of administration were greater than 16 hr, 68 min and 34 min, respectively. in pulmonary mechanics studies in which the effects of i.v. or aerosol administ ...19872824752
reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection.guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ...19872825951
clonorchiasis: an update.the ability of clonorchis sinensis to cause severe hepatobiliary disease is illustrated by the case we have reported, in which biliary obstruction and hepatic fibrosis resulted from the pathologic process associated with chronic clonorchiasis. immunodiagnostic tests are currently limited, and diagnosis, as illustrated by our case, relies on identification of the fluke, usually as the adult or egg stage in clinical specimens. praziquantel has revolutionized therapy of this disease, but surgical d ...19873299730
cytotoxic escherichia coli. 19873305326
monoclonal antibodies that distinguish trypanosoma congolense, t. vivax and t. brucei.monoclonal antibodies (moabs) were derived against in-vitro-propagated procyclic forms of trypanosoma congolense, t. vivax, t. brucei brucei and t.b. rhodesiense in order to identify antigens for use in immunodiagnosis of african trypanosomiasis. the antibodies have been tested against procyclic and bloodstream form trypanosomes of 13 t. congolense, six t. vivax six t.b. brucei, four t.b. rhodesiense, five t.b. gambiense and three t. simiae isolates from different geographical areas by indirect ...19873306569
antibodies against mammary derived growth inhibitor (mdgi) react with a fibroblast growth inhibitor and with heart fatty acid binding protein.a polypeptide growth inhibitor, designated as mammary derived growth inhibitor, has previously been purified from lactating bovine mammary glands. polyclonal rabbit antiserum raised against mammary derived growth inhibitor cross-reacts with bovine heart fatty acid binding protein and bovine peripheral myelin p2 protein. these results are consistent with the observation that the amino acid sequence of mammary derived growth inhibitor showed homology to the sequences of these proteins. in a parall ...19873318832
characterization of a neutralization-sensitive epitope on the am 105 surface protein of anaplasma marginale. 19872448258
short-term starvation in infancy re subsequent learning disabilities--a proven relationship? 19871255342
a comparative study of three different approaches to the comfortable zone.by means of a screw jack device, applied to thirteen edentulous subjects, the comfortable zone was approached in three different ways, by the central, the internal and the external approaches. correspondingly, the zonal borders behaved differently. applying the central approach it was found that the initial height of the screw jack had a lingering effect upon subsequent adjustments of the screw. moreover, lower border data collected by the external approach distinguished themselves from other zo ...1987266049
wpw syndrome: a case study. 1988259431
fighting illegal dentistry--a public health perspective. 1988266484
differential extraction of antigens of anaplasma marginale.strain-, group-, and genus-specific antigens of the florida isolate of anaplasma marginale had different solubilities in zwitterionic, nonionic, and anionic detergents. on the basis of their solubility in nonionic detergent, antigens were grouped into 3 classes: (i) a 108-kilodalton (kd) group-specific antigen and a series of poorly defined antigens (70 to 100 kd) were completely soluble in nonionic detergent; (ii) 96 kd and 75 kd group-specific antigens, and a 91-kd strain-specific antigen were ...19882450497
nucleic acid levels in anaplasma marginale during experimentally induced bovine anaplasmosis.the dna and rna concentrations in uninfected and anaplasma marginale-infected bovine erythrocytes were determined. bovine anaplasmosis was experimentally induced and the nucleic acid levels were followed during the development of the a. marginale infection. two distinct growth stages were found, the "multiplication" stage and the "transfer" stage. based on this hypothetycal developmental cycle, the amount of dna and rna per anaplasma initial body was estimated as 73.1 x 10(-3) pg and 45.7 x 10(- ...19882451913
recognition of conserved surface protein epitopes on anaplasma centrale and anaplasma marginale isolates from israel, kenya and the united states. 19882452797
preliminary studies of the development of anaplasma marginale in salivary glands of adult, feeding dermacentor andersoni ticks.on each day of feeding on susceptible calves, salivary glands obtained from groups of adult ticks that transmitted anaplasma marginale were examined for a marginale colonies by use of light microscopy and transmission electron microscopy. on day 8 of feeding, salivary glands were examined, using fluorescein-labeled antibody and methyl green-pyronine stain. use of fluorescein-labeled antibody consistently revealed small numbers of fluorescent foci in salivary gland acinar cells obtained from tick ...19882458688
medium conditioned by spleen cells of nematospiroides dubius-infected mice does not support development of cultured mast cells.the ability of different conditioned media to support mast cell development from precursors in normal bone marrow was evaluated. many mast cells developed in bone marrow cultures containing medium conditioned by concanavalin-a-stimulated spleen cells of normal mice, trichinella spiralis-infected mice, or mice infected for 6 days by nematospiroides dubius. by contrast, medium conditioned by concanavalin-a-stimulated spleen cells of mice having 18-day infections of the nematode n. dubius failed to ...19883338850
cultivation of anaplasma marginale from cattle in a dermacentor cell line.a tick cell line derived from dermacentor variabilis (rml-15) was inoculated with bovine rbc infected with anaplasma marginale. two hours after inoculation, numerous rbc were phagocytized by the tick cells. after one passage of the cell culture, numerous groups of anaplasma-like particles were seen in the tick cell cytoplasm. increased numbers of anaplasma-like particles also were present. seemingly, anaplasma can multiply in tick cells.19883348535
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