| haemorrhagic septicaemia: correlation of vaccinal antibody responses in mice with protection against pasteurella multocida strain m1404. | this study examined the protection induced by oil adjuvant vaccine and broth bacterin in mice. protective immunity was induced by both oil adjuvant and bacterin vaccination procedures. oil adjuvant vaccination induced a 10(5)-fold increase for lethal challenge over control mice, while secondary vaccination induced a further 10-fold increase in resistance to lethal challenge. broth bacterin induced a slightly weaker protective response with 10(4)- and 10(5)-fold increases in resistance to lethal ... | 1991 | 1909067 |
| structural and functional relationships between pasteurella multocida and enterobacterial adenylate cyclases. | the pasteurella multocida adenylate cyclase gene has been cloned and expressed in escherichia coli. the primary structure of the protein (838 amino acids) deduced from the corresponding nucleotide sequence was compared with that of e. coli. the two enzymes have similar molecular sizes and, based on sequence conservation at the protein level, are likely to be organized in two functional domains: the amino-terminal catalytic domain and the carboxy-terminal regulatory domain. it was shown that p. m ... | 1991 | 1917858 |
| epizootiology of avian cholera in wildfowl. | pasteurella multocida, the cause of avian cholera, has naturally infected over 100 species of free-living birds. among wild birds, avian cholera has its greatest impact on north american wildfowl. epizootics usually are explosive in onset and may involve thousands of birds. the disease has been reported in every month of the year among wildfowl. disproportionate mortality, with some species suffering proportionately greater mortality than others, has been a common feature of this disease. presen ... | 1991 | 1920657 |
| stability of pathogenic bacteria from laboratory animals in various transport media. | the stability of pathogenic bacteria from laboratory animals was investigated in various transport media at different temperatures. bordetella bronchiseptica and salmonella typhimurium survived for 8 days in phosphate-buffered saline (pbs, ph 7.0) at 37, 24, 4 and -20 degrees c; brucella canis at 24, 4 and -20 degrees c; corynebacterium kutscheri at 4 and -20 degrees c; and pseudomonas aeruginosa at all but -20 degrees c. a marked decrease in numbers of pasteurella multocida and past. pneumotrop ... | 1991 | 1921320 |
| naturally acquired pasteurella multocida subsp. multocida infection in a closed colony of rabbits: characteristics of isolates. | twelve litters, comprising 41 rabbits aged 35 to 60 days old, in a closed university colony, were monitored for acquisition of nasal pasteurella multocida subsp. multocida infection. isolates from 11 infected rabbits were characterized by colonial morphology, capsular type, biotype and antibiotic resistance. selected isolates were further characterized by somatic antigen typing. two major strains of p. multocida subsp. multocida were detected in the colony. one strain had mucoid colonies, fermen ... | 1991 | 1921322 |
| female hiv alert. | | 1991 | 1923066 |
| characterization of toxin from different strains of pasteurella multocida serotype a and d. | chromosomal dna from 13 different selected pasteurella multocida spp. multocida strains of serotypes a and d were isolated and compared. all 10 toxigenic strains were recognized by a dna probe which included the toxa gene coding for the pasteurella multocida toxin (pmt). none of the three nontoxigenic strains reacted with the dna probe. toxin from the 10 toxigenic strains were isolated and compared. all were found to possess the biological characteristics previously described for the pmt isolate ... | 1991 | 1927149 |
| molecular distribution of rob-1 beta-lactamase in actinobacillus pleuropneumoniae. | the plasmid content and molecular distribution of the rob-1 beta-lactamase was investigated in 31 swine isolates of actinobacillus pleuropneumoniae. three types of plasmid patterns were observed in ampicillin-resistant isolates: a predominant one consisting of 2.6-, 2.9-, and 5.0-kb plasmids and two other patterns consisting of 4.2- and 5.5-kb plasmids and of a 6.8-kb plasmid. plasmid dna preparations were hybridized with a blarob-1 intragenic fragment from the haemophilus influenzae rrob plasmi ... | 1991 | 1929299 |
| [detection of neutralizing antibodies against pasteurella multocida toxin in swine with atrophic rhinitis]. | blood sera of 1171 pigs, not vaccinated against par, were tested for antibodies against p. multocida toxin in a neutralization test with ebl cell cultures. 277 sera were from 18 herds with clinical par; 104 of them (37.5%) had neutralizing antibody titres from 1:2 to 1:1024. no antitoxin was detected in the sera of 866 pigs in 25 par nonsuspicious herds. in one nonsuspicious herd, however, 11 of 28 sera were neutralizing in the 1:2 or 1:4 dilution. 30 sows had been vaccinated with inactivated to ... | 1991 | 1935682 |
| pasteurella multocida and female genital carcinoma; a case report to dispute the concept of genital opportunistic pathogen. | | 1991 | 1936508 |
| the outer membrane of pasteurella multocida 3:a protects rabbits against homologous challenge. | the protective efficacy of a vaccine purified from the pasteurella multocida 3:a outer membrane (om) was evaluated in rabbits by homologous challenge. twenty-seven rabbits were divided into four groups: 1, vaccinated with om and challenged; 2, nonvaccinated and challenged; 3, vaccinated with om only; and 4, nonvaccinated and not challenged. rabbits were immunized intranasally with 1 mg of om protein on days 0, 7, 14, and 35, challenged intranasally on day 49, and killed on day 63. mortality rate ... | 1991 | 1937811 |
| [creatinine clearance as the foundation for clinical determination of kidney function in swine]. | comparative examinations of renal function using inulin- and endogenous creatinine-clearance tests in 5 pigs showed nearly identical values of glomerular filtration rate (gfr). based on this relation 79 healthy pigs, weighting 2 kg to 230 kg were investigated using the creatinine-clearance test for determination of normal values of gfr and urine flow rate (vu) as well as renal excretion (e), renal clearance (clr) and fractional excretion (fe) of urea, sodium, potassium, calcium, phosphorus, gluc ... | 1991 | 1948984 |
| colonization of the pharyngeal tonsil and respiratory tract of the gnotobiotic pig by a toxigenic strain of pasteurella multocida type d. | seven-day-old gnotobiotic pigs were inoculated intranasally with pasteurella multocida and euthanatized 2, 5, 9, and 14 days after inoculation. tissues from the oropharynx and respiratory tract of pigs were cultured quantitatively and analyzed microscopically. pigs remained afebrile and alert, except one that died of acute fibrinopurulent pneumonia. pasteurella multocida was isolated in greatest numbers from the pharyngeal tonsils, but only in low numbers from turbinate, trachea, lung, spleen, a ... | 1991 | 1949505 |
| an abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. ii. enzootic pneumonia of pigs: microbiological findings and their relationship to pathomorphology. | lungs from 191 slaughter pigs with gross lesions indicative of enzootic pneumonia of pigs (epp) and 80 grossly normal lungs, all originating from 9 different herds, were subjected to microbiological and pathological examinations. the microbiological studies included both bacterial and mycoplasmal culture and also testing for mycoplasma hyopneumoniae antigen in tissue by indirect immunofluorescent technique. m. hyopneumoniae, pasteurella multocida and mycoplasma hyorhinis were detected in 83%, 43 ... | 1991 | 1950853 |
| 99mtc-medronate uptake in the temporomandibular joints of young rats treated with a mandibular hyperpropulsor. | to correct maxillomandibular sagittal discrepancies in growing children, most functional appliances position the mandible more anteriorly than its habitual relation with the maxilla. the aim of this study was to evaluate the effects of such a procedure on the mineralization of the temporomandibular joints in young growing rats. temporomandibular joint uptake of a radioactive bone marker, technetium 99m methylene diphosphonate (99mtc-mdp), was measured over time as a hyperpropulsor appliance was ... | 1991 | 1951199 |
| resistance of pasteurella multocida a:3,4 to phagocytosis by turkey macrophages and heterophils. | a virulent field isolate and 2 vaccine strains of pasteurella multocida a:3,4 were compared for resistance to phagocytosis by turkey macrophages and heterophils, using in vitro assays. the least virulent vaccine strain (m-9) was phagocytosed to a greater degree than was the field isolate or the other vaccine strain (clemson university). all 3 bacteria differed significantly from each other in the amount of capsular material present as measured by polycationic ferritin labeling and electron micro ... | 1991 | 1952342 |
| pasteurellosis in bobwhite quail. | a flock of 5000 six-week-old bobwhite quails (colinus virginianus) experienced high mortality (52%) over a 2-day period. mortality was 99% within a 6-day period. clinical signs were depression followed shortly by death. gross lesions observed in dead quails were congested lungs and, in a few cases, mottled livers. histopathologic examination revealed severe, diffuse, heterophilic interstitial pneumonia and multifocal areas of hepatic and splenic necrosis with numerous intracellular and extracell ... | 1991 | 1953585 |
| [pasteurella multocida as the cause of disease outbreaks in commercial poultry flocks]. | six cases of fowl cholera in growing turkeys and 3 in adult breeder chickens of the broiler type as well as one case each of a pasteurella (p.) multocida-associated disease in ducklings and goslings were described in consideration of own laboratory findings and available informations of the case history. furthermore a report is given on a treatment strategy successfully used in turkeys with highly acute fowl cholera. all the p. multocida strains isolated culturally could be assigned to the subsp ... | 1991 | 1953629 |
| infections resulting from animal bites. | animal bites are a major public health problem. this article reviews the epidemiology and treatment of animal bites. the epidemiology, clinical presentation, and treatment of infections caused by pasteurella multocida and capnocytophaga canimorsus (df-2) are reviewed in detail. | 1991 | 1955705 |
| estimates of quantitative genetic parameters of immunological traits in the chicken. | three in vivo assays were used to measure the immunocompetence of chickens in two generations of a selection experiment. the obtained data were used to estimate the variance components for sire and dam for antibody production to pasteurella multocida and mycoplasma gallisepticum vaccines, for t-cell-mediated immunity evaluated by a phytohemagglutinin wing web assay, and for clearance of foreign particles from the circulatory system. heritabilities of and genetic correlations among these immunolo ... | 1991 | 1956847 |
| immunisation of cattle and buffaloes with live haemorrhagic septicaemia vaccine. | | 1991 | 1957476 |
| evaluation of combined pasteurella vaccines in control of sheep pneumonia. | the effectiveness of an oil adjuvant vaccine (oav) incorporating locally isolated strains of pasteurella haemolytica type 7 and pasteurella multocida types a and d was compared with that of carovax (wellcome laboratories) in imported cross-bred lambs. the criterion of efficacy was the ability of the vaccines to reduce the extent of pneumonic lesions in vaccinated as against unvaccinated control lambs. the oav produced at this institute significantly reduced the lung lesions at p less than 0.05 l ... | 1991 | 1959015 |
| in-vitro activities of penicillin, amoxycillin and certain cephalosporins, including cefpodoxime, against human isolates of pasteurella multocida. | | 1991 | 1960130 |
| evidence that arachidonic acid influences hen granulosa cell steroidogenesis and plasminogen activator activity by a protein kinase c-independent mechanism. | we recently proposed that arachidonic acid serves as a second messenger within granulosa cells from the largest preovulatory follicle of the hen. the present studies were conducted to determine whether the inhibitory effects of arachidonic acid on lh-induced camp accumulation and on the ability of cells to convert 25-hydroxycholesterol to progesterone are mediated via the protein kinase c pathway. furthermore, we determined the effects of arachidonic acid on plasminogen activator activity in gra ... | 1990 | 1963326 |
| h(+)-stimulated release of prostaglandin e2 and cyclic adenosine 3',5'-monophosphoric acid and their relationship to bone resorption in neonatal mouse calvaria cultures. | the addition of protons to the medium of neonatal mouse calvaria cultures stimulated bone resorption and release of calcium into the medium. in addition, added protons significantly increased the release of prostaglandin e2 (pge2) and cyclic adenosine 3',5'-monophosphoric acid (camp) from the bones. indomethacin significantly inhibited the release of calcium, pge2 and camp from proton-treated cultures. the positive control, parathyroid hormone (pth)-treated cultures, also gave rise to bone resor ... | 1990 | 1964815 |
| drug-susceptibility and isolation of a plasmid in haemophilus (actinobacillus) pleuropneumoniae. | we isolated 56 haemophilus (actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. two drug-resistant strains were obtained. one, named kh-265, was resistant to streptomycin (sm) and sulfonamide (sa), and the other, named kh-195, was resistant to tetracycline (tc). the minimum inhibitory concentrations (mics) of drugs for resistant strains were 100 micrograms/mliters for sm, 3200 micrograms/mliters for sa, and 12.5 micro ... | 1990 | 1968988 |
| [pasteurella multocida (type d and a) and atrophic rhinitis of swine--hemagglutination, fimbriae and adhesion in vitro in the nasal mucosa of swine fetuses]. | 79 strains of p. multocida were investigated, mostly isolated from porcine nasal cavities, and a mannose-resistant hemagglutination with guinea pig and human group 0, but not with porcine erythrocytes was found. fimbriae as adhesins were demonstrated only on 2 strains. a correlation between capsular type, hemagglutination, fimbriation and toxigenicity on the examined p. multocida strains was not observed. three strains were investigated for the adherence to the nasal mucosa of neonatal pigs; agg ... | 1990 | 1980059 |
| a monoclonal antibody against a pasteurella multocida outer membrane protein protects rabbits and mice against pasteurellosis. | monoclonal antibodies (mabs) directed against the 37.5-kda outer membrane protein were produced by fusing myeloma cells with spleen cells obtained from mice immunized with a pathogenic strain of pasteurella multocida isolated from a rabbit. desirable mabs were selected by enzyme-linked immunosorbent assay, whole-cell radioimmunoprecipitation (wc-rip), and western blot (immunoblot) analysis. wc-rip and western blot analyses, using mab 1608 adsorbed with intact p. multocida cells and the eluted ma ... | 1991 | 1987031 |
| freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles. | freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ... | 1991 | 1999383 |
| pasteurella multocida toxin, a potent mitogen, increases inositol 1,4,5-trisphosphate and mobilizes ca2+ in swiss 3t3 cells. | pasteurella multocida toxin, both native and recombinant, is an extremely potent mitogen for swiss 3t3 cells and acts to enhance the formation of total inositol phosphates (rozengurt, e., higgins, t., changer, n., lax, a.j., and staddon, j.m. (1990) proc. natl. acad. sci. u.s.a. 87, 123-127). p. multocida toxin also stimulates diacylglycerol production and activates protein kinase c (staddon, j.m., chanter, n., lax, a.j., higgins, t.e., and rozengurt, e. (1990) j. biol. chem. 265, 11841-11848). ... | 1991 | 2002031 |
| [pasteurella multocida infections: bites by dogs or cats?]. | annually 50,000 to 100,000 animal bites are seen by physicians in the netherlands. infections of these bite wounds frequently occur. pasteurella multocida is one of the main causes of these infections and many serious complications may occur. we present 3 patients with disturbances in wound healing after animal bites or scratches due to infection with p. multocida. in view of the high infection ratio after cat bites we advise giving amoxicillin/clavulanic acid (augmentin) for 5 days as prophylax ... | 1991 | 2002857 |
| antibodies to outer membrane proteins but not to lipopolysaccharide inhibit pulmonary proliferation of pasteurella multocida in mice. | the role of rabbit antibodies against pasteurella multocida outer membrane proteins and lipopolysaccharides (lps) in resistance remains unknown. pooled immune sera against p. multocida outer membranes were prepared from specific-pathogen-free rabbits immunized with sucrose gradient-purified p. multocida outer membranes. western immunoblotting showed that purified outer membrane protein antibodies reacted strongly against the outer membrane proteins but not the purified lps. affinity-purified lps ... | 1991 | 2004825 |
| case control study of fowl cholera in meat turkeys in california from august 1985 through july 1986 [corrected]. | from aug 1985 through july 1986, 720 meat turkey flocks on 160 california premises were monitored and outbreaks of fowl cholera (pasteurella multocida) were investigated. data from 43 outbreak (case) flocks were compared with data from 43 nonoutbreak (control) flocks. outbreak flocks, compared with control flocks, were more likely to be located on premises with higher maximal bird capacity and history of fowl cholera outbreaks. the overall impression was that flocks in larger, newer, more intens ... | 1991 | 2012330 |
| meningitis due to pasteurella multocida. | | 1991 | 2017627 |
| acute airsacculitis in turkeys inoculated with cell-free culture filtrate of pasteurella multocida. | twenty-six female and 26 male turkeys, inoculated into the caudal thoracic air sacs with cell-free culture filtrate of pasteurella multocida strain r44/6, were examined from 0 to 6 hours post-inoculation and compared with 26 female and 26 male sham-inoculated control turkeys given brain-heart-infusion broth. the air sac reacted rapidly with exudation of heterophils. microscopically, low numbers of heterophils were present within air sac blood vessels and also perivascularly by 0.5 hour after ino ... | 1991 | 2017827 |
| [late post-pneumonectomy hematogenous pyothorax. apropos of 3 cases]. | the authors describe three cases of patients who had previously undergone a pneumonectomy (two for cancer, one for bronchiectasis) with a favourable postoperative course, who subsequently developed pyothorax in the thoracic cavity. the bacteria isolated where in the first patient, pasteurella multocida; in the second, campylobacter foetus foetus; in the third, a variety of anaerobic organisms. the characteristic of the organisms allow us to exclude the possibility that these cases of pyothorax w ... | 1991 | 2018342 |
| determination of affinity of pasteurella multocida isolates for porcine respiratory tract mucus, and partial characterization of the receptors. | the ability of 25 pasteurella multocida isolates to adhere in vitro to porcine respiratory tract mucus was examined. microplate wells were coated with crude mucus preparation, then bacteria were added. after incubation, unbound bacteria were removed by washing, and the number of mucus-bound bacteria was estimated by quantitation of the adherent colony-forming units and by use of an elisa. pasteurella multocida had affinity to respiratory tract mucus, although significant differences were not obs ... | 1991 | 2021250 |
| aerobic bacteria from nasal discharge of goats suffering from clinical ppr: isolation and identification. | bacteriological isolation of aerobic bacteria from nasal discharge of achondroplastic west african dwarf goats showing clinical signs of peste des petits ruminants (ppr), and the laboratory procedures carried out for the identification of the isolates are described. from a total of forty nasal swabs obtained seventy five isolates were recovered. the percentage frequency of isolation of different organisms in descending order of occurrence is as follows: staphylococcus 44.0%; streptococcus 22.67% ... | 1991 | 2023545 |
| resistance to trimethoprim and 2,4-diamino-6,7-diisopropyl-pteridine (0/129) in pasteurella haemolytica. | thirteen strains of pasteurella haemolytica resistant to moderate levels of trimethoprim (mics from 8 to 64 micrograms/ml) and 0/129 (mics from 16 to 64 micrograms/ml) were isolated from bovine specimens. two strains, cnp330 and cnp334, were studied and found to harbour various plasmids but all attempts to cure trimethoprim resistance were unsuccessful. resistance characters were not transferable to escherichia coli or to pasteurella multocida by conjugation and to e. coli by transformation. the ... | 1991 | 2024433 |
| reclassification of german, british and dutch isolates of so-called pasteurella multocida obtained from pneumonic calf lungs. | the taxonomic relationship of 131 strains previously identified as pasteurella multocida obtained from calf pneumonia in west germany, united kingdom and netherlands was investigated by extended phenotypic and limited genotypic characterization. twenty-four strains were classified as p. multocida ssp. multocida, 15 strains as p. avium biovar 2 and 13 strains as p. canis biovar 2. sixty-five and five strains were tentatively classified as ornithine negative p. multocida ssp. multocida and p. mult ... | 1991 | 2024434 |
| a survey of potential virulence markers from avian strains of pasteurella multocida. | twenty-four isolates of pasteurella multocida from clinical cases of fowl cholera and the clemson university vaccine strain were surveyed for the presence of potential virulence markers. membrane proteins, enzymatic activity of the membrane proteins, and carbohydrate fermentation patterns were also determined to demonstrate phenotypic relationships within the groups. few differences were found in these phenotypic characteristics among the isolates. almost all the organisms produced siderophore a ... | 1991 | 2024443 |
| vaccination of turkeys with cell-free culture filtrate of pasteurella multocida. | turkeys given cell-free culture filtrate (ccf) of pasteurella multocida strain r44/6 orally, via air sacs, or subcutaneously mixed 1:1 with incomplete freund's adjuvant (ifa) at 6 and 9.5 weeks of age were compared with negative controls given bacteriologic medium and positive controls vaccinated with a commercial bacterin. at 13 weeks of age, serum antibody titers to p. multocida were detectable only in turkeys given ccf in ifa (low titers) and positive control turkeys (high titers), at which t ... | 1991 | 2029246 |
| effects of adjuvant-augmented germling vaccines in turkey poults challenged with aspergillus fumigatus. | turkey poults were vaccinated with combinations of two different germling preparations and three adjuvants (n-acetylmuranyl-l-alanyl-d-isoglutamine, pasteurella multocida lipopolysaccharide [lps], and avridine) at 1 and 2 weeks of age, and their immunity was challenged by sublethal exposure to aerosols of aspergillus fumigatus conidia at 1 month of age. fewer turkeys in the groups given vaccines prepared from germlings grown on dorset's and henley's medium (d&h) had organisms in lung tissue at 2 ... | 1991 | 2029265 |
| development of pneumonia in desert bighorn sheep after exposure to a flock of exotic wild and domestic sheep. | from 1986 to 1989, 5 desert bighorn sheep (3 ovis canadensis mexicana and 2 o c nelsoni), ranging in age from 2 to 3 years, were exposed to a flock of exotic wild and domestic sheep to potentially achieve naturally acquired pneumonia. pasteurella multocida was isolated from nasal samples from 4 of 6 sheep randomly sampled from the flock. bighorn sheep were exposed individually and each exposure period was a trial. treatment before and after exposure varied and included combinations of alpha inte ... | 1991 | 2032914 |
| the cytotoxin of actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kda polypeptide. | mutants of actinobacillus pleuropneumoniae strain hk 361 (serotype 2) were isolated which were deficient in type ii (ca2(+)-dependent) haemolysin activity (hly-). some of the hly- mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. comparison of cell-free culture supernatant from the parent strain and some hly- mutants by sds-page and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kda. two hly- mutants which ... | 1991 | 2033378 |
| telescoping plugged catheter. an unusual way of diagnosing pasteurella multocida pneumonia. | we report a case of pasteurella multocida pneumonia and its unusual clinical presentation. we also discuss the rarity of its diagnosis by tpc specimens and the delay in both an adequate diagnosis and treatment. | 1991 | 2036840 |
| comparison and analysis of the nucleotide sequences of pilin genes from haemophilus influenzae type b strains eagan and m43. | previous studies have demonstrated antigenic differences among the pili expressed by various strains of haemophilus influenzae type b (hib). in order to understand the molecular basis for these differences, the structural gene for pilin was cloned from hib strain eagan (p+) and the nucleotide sequence was compared to those of strains m43 (p+) and 770235 b0f+, which had been previously determined. the pilin gene of hib strain eagan (p+) had a 648-bp open reading frame that encoded a 20-amino-acid ... | 1991 | 2037360 |
| sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility. | | 1991 | 2044514 |
| pasteurella multocida peritonitis in an hiv-positive patient on continuous cycling peritoneal dialysis. | pasteurella multocida has been reported only once previously as a cause of peritonitis in a patient undergoing chronic peritoneal dialysis. the present report describes findings associated with a case of p. multocida peritonitis in a human immunodeficiency virus (hiv)-positive patient in which renal replacement therapy consisted of continuous cycling peritoneal dialysis. to our knowledge this is the first report of this unique infection in an hiv-positive end-stage renal disease patient. in addi ... | 1991 | 2048580 |
| [the energetic utilization of sugar beets, dried chips, cane sugar and apple pectin by growing swine. 2. nitrogen and energy metabolism]. | the energetic utilization of rations with raw and steamed sugar beets, dried sugar beet pulp (16 measured values each), sucrose and apple pectin (8 measured values each) as supplement feed to a basic ration was measured at 4 growing pigs each in the live weight range of between 40 and 130 kg. the utilization of the energy in the rations amounted to 69.1 +/- 5.5, 68.3 +/- 7.1, 60.9 +/- 5.8, 70.5 +/- 4.1 and 69.9 +/- 6.9% (same sequence as above). the utilization values for the supplemented feedst ... | 1991 | 2048968 |
| dermonecrotic toxin production by strains of pasteurella multocida isolated from man. | ninety-four clinical isolates of pasteurella multocida of human origin were tested for dermonecrotic toxin (dnt) production by three methods: dermonecrotic test in guinea-pigs, vero cell culture cytotoxicity and elisa. the strains were isolated from patients living in a rural area with widespread intensive pig breeding. six strains were found to be toxigenic by the three tests. a major protein band of mr 145 kda corresponding to dnt on immunoblots was demonstrated in extracts from these strains. ... | 1991 | 2056517 |
| [oligoarthritis in pasteurella multocida inoculation pasteurellosis]. | | 1991 | 2057694 |
| immunity induced in rats vaccinated with toxoid prepared from heat-labile toxin produced by pasteurella multocida serogroup d. | rats were vaccinated with a toxoid (d-toxoid) prepared from purified heat-labile toxin (d-toxin) produced by pasteurella multocida serogroup d. vaccination of rats with d-toxoid prevented death and other effects of d-toxin (hepatic necrosis, development of elevated leukocyte counts, lymphopenia, neutrophilia, and elevated complement titers) that occurred in phosphate buffered saline (pbs)-vaccinated control rats. | 1991 | 2063548 |
| immunity to pasteurella multocida in protein-deficient chickens. | studies were conducted to determine the effects of dietary protein restriction on the humoral immunity (hi) and cell-mediated immunity (cmi) of chickens. new hampshire chickens were separated into two dietary treatment groups: basal, containing 3,200 kcal/kg and 21% protein; or protein restricted (pr), containing 3,200 kcal/kg and 7% protein. in studies involving hi, half of the birds in each dietary treatment were vaccinated against fowl cholera at 4 and 8 wk of age. blood samples were collecte ... | 1990 | 2084673 |
| pasteurella multocida infective endocarditis: a case report. | a 17-year-old man presented with acute febrile illness with jaundice, embolic skin lesion, heart murmur, renal insufficiency and abnormal csf. pasteurella multocida was isolated from blood cultures. in spite of adequate antibiotic treatment for endocarditis of the mitral valve, he developed a fatal ruptured cerebral mycotic aneurysm. post mortem examination revealed an atrial septal defect, vegetation at the anterior mitral leaflet, intraventricular, subarachnoid and intracerebral hemorrhage. | 1990 | 2086720 |
| [infection caused by pasteurella multocida after a lion bite]. | | 1990 | 2090239 |
| antimicrobial susceptibility of pasteurella multocida type d from missouri swine. | | 1990 | 2090275 |
| [septic arthritis due to pasteurella multocida]. | | 1990 | 2091119 |
| competitive inhibition of a tumour cell surface protease. a rapid technique for in vitro testing of selective targeting systems. | the active centre of a protease on the surface of tumour cells can be located by its affinity for an active site-directed inhibitor, 9-amino acridine. cells which have uninhibited proteases, bind 9-amino acridine and fluoresce in resin sections. the leukaemic rat was used as a model system to provide tumour cells in a well defined location. drugs when coupled to a ligand (directed to the active centre of the protease) compete for this binding site with 9-amino acridine. thus, competitive inhibit ... | 1990 | 2094772 |
| immunopathological changes in mice caused by bordetella bronchiseptica and pasteurella multocida. | the immunopathological changes induced by toxigenic pasteurella multocida, toxigenic phase i bordetella bronchiseptica and its phase iii variant were studied. four groups of mice, each containing 21 animals, were inoculated intravenously with sublethal doses of b. bronchiseptica as follows: group 1: phase i toxigenic b. bronchiseptica whole-cell suspension (wcs); group 2: phase iii b. bronchiseptica wcs; group 3: phase i b. bronchiseptica sonicated extract (se); and group 4: phase iii b. bronchi ... | 1990 | 2099605 |
| the pathology of experimental respiratory infection with pasteurella multocida and bordetella bronchiseptica in rabbits. | groups of female new zealand white rabbits, 8-10 weeks old, were inoculated intranasally with three different pasteurella multocida serotypes (a:3, a:4 and a:12) or one of three bordetella bronchiseptica strains of rabbit origin. seven out of 18 rabbits died of experimental infection with p. multocida. b. bronchiseptica killed 3 out of the 8 animals inoculated with it. deaths occurred between 3 and 6 days postinoculation (pi). in the rabbits that died of p. multocida inoculation, necropsy and hi ... | 1990 | 2099606 |
| survey of actinobacillus (haemophilus) pleuropneumoniae infection in swine by different methods. | lung and serum samples from pigs that died or were emergency-slaughtered in a pooled, conventional fattening herd were examined to survey actinobacillus pleuro-pneumoniae infection and to compare the sensitivity of different testing methods. a total of 110 lungs were used for cultural isolation of the agent and direct immunofluorescence (if) of impression smears. boiled lung suspensions were tested by coagglutination (co-a) and agar gel precipitation (agp). eighty-seven sera were tested along wi ... | 1990 | 2099609 |
| demonstration of pasteurella multocida type 6:b (b:2) in formalin-fixed paraffin-embedded tissues of buffaloes by the peroxidase anti-peroxidase (pap) technique. | | 1990 | 2099627 |
| [nonspecific immunostimulating activity of bordetella bronchiseptica bacterin in tests on mice and calves]. | three experimental models were used to verify the effectiveness of immunostimulant preparations of microbial origin. of the four microbial species, used in the tests with the stimulation of mice against subsequent infection with the virulent strain of pasteurella multocida, the highest resistance-increasing power was recorded in bordetella bronchiseptica. there were large differences in stimulation activity between its strains, ranging from those with a low activity up to strain 6229, which incr ... | 1990 | 2100431 |
| pasteurella multocida infections in mice with reference to haemorrhagic septicaemia in cattle and buffalo. | haemorrhagic septicaemia (hs) is an infectious disease of cattle and buffalo caused by particular serotypes of pasteurella multocida and is one of the most economically important livestock diseases in south-east asia. while hs has been recognized for many years, very little is understood about the disease, primarily because of the expense of cattle and a lack of suitable animal models. the suitability of using mice to study hs was assessed using parameters such as the critical pathogenic dose, k ... | 1990 | 2108081 |
| effects of haemorrhagic septicaemia vaccination and levamisole administration on the humoral response in cross-bred calves. | levamisole administered by oral, subcutaneous or transdermal routes following vaccination against pasteurella multocida enhanced the humoral response in cross-bred calves. the group that received levamisole subcutaneously elicited highly significant (p less than 0.01) antibody titres during the primary humoral response in comparison with groups that received levamisole orally or transdermally. during the secondary response in the same experiment, levamisole administered subcutaneously and transd ... | 1990 | 2108257 |
| pasteurella multocida otitis media complicated by facial palsy. | | 1990 | 2108421 |
| atypical pasteurella strains producing a toxin similar to the dermonecrotic toxin of pasteurella multocida subspecies multocida. | this paper is the first report of the production of a dermonecrotic toxin by pasteurella strains that do not belong to the species pasteurella multocida subspecies multocida. four strains, isolated from cattle with atrophic rhinitis, were characterised phenotypically. the strains were related to pasteurellaceae, but their taxonomic position remained unclear. the strains produced a toxin that caused a haemorrhagic dermonecrosis in guinea pigs and was lethal to mice. both effects were neutralised ... | 1990 | 2111955 |
| multivariate analysis of quantitative chemical and enzymic characterization data in classification of actinobacillus, haemophilus and pasteurella spp. | chemotaxonomic data for strains of actinobacillus, haemophilus and pasteurella spp. were analysed using three multivariate statistical strategies: principal components, partial least squares discriminant, and soft independent modelling of class analogy. the species comprised actinobacillus actinomycetemcomitans. haemophilus aphrophilus, h. paraphrophilus, h. influenzae, pasteurella multocida, p. haemolytica and p. ureae. strains were characterized by cell sugar and fatty acid composition, lysis ... | 1990 | 2118166 |
| persistence of the carrier status in haemorrhagic septicaemia (pasteurella multocida serotype 6:b infection) in buffaloes. | fifty-seven young buffaloes were experimentally infected or naturally exposed to haemorrhagic septicaemia (hs). of these animals 32 became immune carriers. they were observed in groups for periods up to 360 days to monitor the appearance of pasteurellae in the nasopharynx and antibody status. in most animals pasteurellae appeared in the nasopharynx for a short period initially and disappeared. the organism reappeared intermittently and the longest observed period of reappearance was 215 days aft ... | 1990 | 2120825 |
| pasteurella multocida osteomyelitis of the cervical spine in a patient on chronic haemodialysis. | osteomyelitis due to pasteurella multocida has been frequently documented but virtually all previous cases have resulted from direct inoculation of the organism or contiguous spread of local infection, following animal bites or scratches. infections often occur in patients with serious underlying illnesses. haematogenous osteomyelitis due to p multocida has very rarely been reported particularly in patients with chronic renal failure. we describe a patient on chronic haemodialysis who developed ... | 1990 | 2124004 |
| rapid identification of pasteurella multocida organisms responsible for haemorrhagic septicaemia using an enzyme-linked immunosorbent assay. | haemorrhagic septicaemia (hs) is caused by specific serotypes of pasteurella multocida and is one of the major economic diseases of cattle and buffalo in south east asia. definitive diagnosis of the disease-causing organism with the available methods is labour intensive and not totally reliable, consequently, an elisa system to identify p multocida organisms which cause hs was developed. one hundred and twenty-four p multocida isolates were tested, 58 were type strains and 66 were field isolates ... | 1990 | 2125138 |
| in vitro activity of bay v 3522, a new oral cephalosporin. | the in vitro activity of bay v 3522 was compared with the activities of cephalexin, cefaclor, cefuroxime, cefixime, and amoxicillin-clavulanate. mics (in micrograms/ml) of bay v 3522 were as follows: staphylococcus spp. (except for oxacillin-resistant strains), 0.13 to 1; streptococcus spp. (except for some viridans group streptococci), less than or equal to 0.015 to 0.25; enterococcus faecalis, 2 to 8; other enterococci, 0.5 to greater than 32; beta-lactamase-negative haemophilus influenzae and ... | 1990 | 2126697 |
| bacterial adhesion to mucosal surfaces with special reference to pasteurella multocida isolates from atrophic rhinitis. | adhesion to mucosal cells is an important virulence attribute of bacterial pathogens colonizing these sites. bacteria of the upper respiratory system, such as members of the genus bordetella, have well-defined adhesins. the main adhesin of b. pertussis is the filamentous hemagglutinin which can be used by other bacteria for attachment. the main adhesin of b. bronchiseptica is the bovine erythrocyte hemagglutinin. in both bordetella species the presence of fimbriae does not appear critical to adh ... | 1990 | 2141799 |
| [the effect of local and systemic immunization of suckling pigs on bronchoalveolar clearance and on intrabronchial infections with pasteurella multocida]. | in vivo investigations of bactericidal bronchoalveolar clearance of weaning pigs locally or systemically immunized with pasteurella antigens exhibited clearly distinctive results, as early as 3 days after the last immunization. they differed from controls by significant increase in the elimination of the homologous pasteurella multocida a wild strain. that increase in the clearance remains for 21 days and is specific only to the immunized capsular type. there are no relations of this result to t ... | 1990 | 2143646 |
| clinical and pathological effects of the dermonecrotic toxin of bordetella bronchiseptica and pasteurella multocida in specific-pathogen-free piglets. | the role of dermonecrotic toxin (dnt) of bordetella bronchiseptica and pasteurella multocida, purified by repeated chromatography in sephacryl s-200 gel, in the pathogenesis of atrophic rhinitis (ar) of swine was studied bacteriologically, clinically and pathologically. two-week-old specific pathogen-free (spf) piglets were parenterally treated with 30 micrograms of dnt 3 times at 2-day interval and 7-week-old piglets were treated with 15 micrograms of dnt twice a week for 5 weeks. in 2- to 3-we ... | 1990 | 2144027 |
| the influence of growth conditions of pasteurella multocida on its ability to colonise the nasal mucosa of spf piglets. | colonisation of type d pasteurella multocida was studied in five groups of seven spf piglets each. piglets of group 1 were kept together with seven 5-week-old piglets obtained from a large herd infected with toxigenic p. multocida for 16 weeks (contact infection). these piglets were made free from toxigenic bordetella bronchiseptica by local immunisation. piglets of group 2 were inoculated with 5 x 10(7) colony-forming units (cfu) of p. multocida washed from the nasal mucosa of piglets free from ... | 1990 | 2145686 |
| colonisation by pasteurella multocida in atrophic rhinitis of pigs and immunity to the osteolytic toxin. | gnotobiotic pig antisera to purified toxoid from a capsule type a or d strain of pasteurella multocida contained large quantities of antitoxin but comparatively little antibody to a crude lysate of p. multocida. these sera given intraperitoneally to further pigs were almost completely protective against turbinate atrophy after intranasal inoculation of dilute acetic acid and infection with type d toxigenic p. multocida. in contrast, antisera to a crude lysate or bacterin of toxigenic p. multocid ... | 1990 | 2149226 |
| characterization of pasteurella multocida mutants of low virulence. | ten temperature-sensitive mutants of the clemson university (cu) vaccine strain of pasteurella multocida have been developed and were characterized by phenotypic attributes such as carbohydrate fermentation, antibiotic resistance, and membrane protein profiles. some mutants were found to have lost the ability to utilize some substrates, notably xylose and gluconate, whereas others were able to ferment additional carbohydrates such as arabinose and rhamnose. cu was found to be resistant to sulfis ... | 1990 | 2149262 |
| pasteurella multocida toxin: potent mitogen for cultured fibroblasts. | native pasteurella multocida toxin (pmt) is shown to be an extremely potent mitogen for swiss 3t3 fibroblasts. half-maximal stimulation of dna synthesis was obtained at concentrations of 1 and 2 pm for recombinant pmt (rpmt) and pmt, respectively. the degree of rpmt-induced dna synthesis was comparable to that elicited by 10% fetal bovine serum and, moreover, was observed in the complete absence of other factors. cell proliferation was also enhanced by rpmt. the toxin was also a potent mitogen f ... | 1990 | 2153282 |
| dna fingerprinting for differentiation of field isolates from reference vaccine strains of pasteurella multocida in turkeys. | the genomes from field isolates of pasteurella multocida in turkeys and those of p multocida reference cu and m9 vaccine strains were analyzed and compared after cleavage with restriction endonucleases. the electrophoretic profiles obtained with dna fragments from field isolates and vaccine strains of the same serotype were characteristic and reproducible. these features indicated the existence of differences among the isolates of the same serotype that cannot currently be detected, using availa ... | 1990 | 2154149 |
| tn7 inserts in both orientations at a single chromosomal location and apparently forms cointegrates in pasteurella multocida. | pasteurella multocida transconjugants isolated after mating with escherichia coli strains that carry one or the other of two tn7-containing suicide plasmids, prktv5 and puw964 (prktv5::tn5), were analysed. these plasmids have the cole1 replication origin and were thus expected to deliver transposons but not be maintained as free replicons in pasteurella. five out of six transconjugants selected for acquisition of tn7 from e. coli (prktv5) had simple insertions of the transposon, in either orient ... | 1990 | 2157128 |
| naturally acquired pasteurella multocida infection in rabbits: immunological aspects. | natural infection with p. multocida in new zealand white rabbits was followed in 2 to 3 weeks by development of immunoglobulin (ig)m and igg serum antibodies. the igm response peaked and returned to lower levels within several weeks after infection, whereas the igg response progressively increased and remained elevated. | 1990 | 2162985 |
| establishment of a camptothecin analogue (cpt-11)-resistant cell line of human non-small cell lung cancer: characterization and mechanism of resistance. | camptothecin-11 (cpt-11) is a new derivative of camptothecin, a plant alkaloid antitumor agent, and a good candidate for clinical trials because of higher antitumor activity, less toxicity, and high aqueous solubility. cpt-11 is known to be altered into an active form, sn-38, by esterase in in vivo. cpt-11-resistant cells (pc-7/cpt) established from a human non-small cell lung cancer cell (pc-7) by stepwise, continuous treatment with cpt-11 exhibit about a 10-fold increase in resistance to the d ... | 1990 | 2168285 |
| integration host factor increases the transpositional immunity conferred by gamma delta ends. | the ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (ihf). ihf+ and ihf- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for ihf binding to determine the role that ihf plays in various gamma delta-mediated transposition events. ihf was not essential for the transposition of gamma delta and seemed to decrease its frequency of transposition about threefold. ihf playe ... | 1990 | 2168370 |
| sequence analysis of conserved rega and variable orf43.1 genes in t4-like bacteriophages. | bacteriophage t4 rega protein is a translational repressor of several phage mrnas. in the t4-related phages examined, rega nucleotide sequences are highly conserved and the inferred amino acid sequences are identical. the exceptional phage, rb69, did not produce a rega protein reproducibly identifiable by western blots (immunoblots) nor did it produce mrna that hybridized to t4 rega primers. nucleotide sequences of either 223 or 250 base pairs were identified immediately 3' to rega in rb18 and r ... | 1990 | 2168375 |
| beta adrenergic binding sites in the human eye: an autoradiographic study. | beta adrenergic binding sites were localized and characterized in the human eye by means of "in vitro" autoradiography, using [125i] (-) iodocyanopindolol (125icyp) as radioligand. binding sites were visualized by apposition of isotope sensitive film to slide mounted eye sections. receptor sites were present in the extraocular muscles, in the conjunctiva, in the epithelium and endothelium of the cornea, in the trabeculum and in the ciliary muscle. they were also present in the lens epithelium an ... | 1990 | 2168462 |
| protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves. | formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ... | 1990 | 2168606 |
| serological surveillance for antibodies against different avian infectious agents on turkey flocks naturally infected with turkey rhinotracheitis. | turkey rhinotracheitis (trt) in the state of baden-württemberg, germany appears to have become endemic affecting all farms and every new restocking. in 5 meat turkey flocks, serological surveillance for antibodies to trt-, ib- and ibd-viruses, as well as to pasteurella multocida were carried out using elisa tests. furthermore, sera were examined for the presence of antibodies to avian adenovirus (celo virus), reo- and paramyxo-viruses 1 and 3. the birds were bled at 4 week intervals starting at ... | 1990 | 2168612 |
| effect of particle size and temperature on the conformation and physiological behavior of apolipoprotein e bound to model lipoprotein particles. | the effect of particle size and structural order/disorder of the lipid domain on the conformation and physiological behavior of lipid-associated apolipoprotein e (apoe) was evaluated. circular dichroic (cd) spectra of apoe bound to large (lme) and small (sme) microemulsion particles, composed of dimyristoylphosphatidylcholine (dmpc) and cholesteryl oleate (co), and to dmpc disks revealed that at 4 degrees c, where all of the lipid constituents were in an ordered state, apoe bound to lme displaye ... | 1990 | 2168737 |
| eicosanoid receptors of the gastrointestinal tract. | | 1990 | 2169178 |
| detection of bovine viral diarrhea virus in serum from cattle by dot blot hybridization assay. | a dot blot hybridization assay was developed for use as a rapid screening test to detect bovine viral diarrhea virus (bvdv) in serum from infected cattle. a 1.1. kilobase cdna, prepared from the bvdv genome, was molecularly cloned and used in this study. insert cdna was removed from the puc9 plasmid vector by pst-i restriction endonuclease digestion and purified from plasmid dna by agarose electrophoresis and electroelution. the hybridization probe was prepared by nick translation in the presenc ... | 1990 | 2175527 |
| monoclonal antibodies against surface antigens of pasteurella multocida strain p-1059. | four monoclonal antibodies (mabs) were developed against serotype 3:a, p-1059 strain of pasteurella multocida. enzyme-linked immunosorbent assays were used to screen those hybridomas producing antibodies to either a surface protective (2.5 s) or lipopolysaccharide (lps) antigen. mabs 6ee11, d7h10, e11e3, and c11h2 were positive against 2.5 s antigen, and two of them, e11e3 and c11h2, were positive for the lps antigen. mabs 6ee11 and d7h10 reacted with a major protein band of molecular weight of ... | 1990 | 2181995 |
| possession of identical nonconjugative plasmids by different isolates of pasteurella multocida does not imply clonality. | experiments were performed to test the hypothesis that possession of the same nonconjugative r plasmid by different isolates of pasteurella multocida implied that they were of the same clone. seven isolates of p. multocida were studied, two possessed an identical nonconjugative r plasmid (pvm109), four possessed another (pvm110), and one isolate possessed a nonconjugative r plasmid related to pvm110. phenotypic and genotypic characteristics of the isolates were determined and compared. isolates ... | 1990 | 2186566 |
| pasteurella multocida meningitis in an adult: case report and review. | pasteurella multocida is a rare cause of adult meningitis. close animal contact prior to onset of illness is frequent and represents the usual mode of introduction of the organism. in reports of a total of 21 cases of p. multocida meningitis in adults (this case report and 20 described previously in the english-language literature), 18 researchers commented on the occurrence of animal contact: two cases (11%) involved cat bite, 13 (72%) involved animal contact without bite, and three (17%) occur ... | 1990 | 2193351 |
| interactions of haemophilus-actinobacillus-pasteurella bacteria with phagocytic cells. | the haemophilus-actinobacillus-pasteurella (hap) group of bacteria contains a number of important veterinary and human pathogens. although each species has specific characteristics and host range, most share the general property of being resistant to cellular defense mechanisms. in some cases (e.g. pasteurella multocida, pasteurella haemolytica and actinobacillus pleuropneumoniae) resistance results in part from the presence of an antiphagocytic capsule that protects the bacilli against ingestio ... | 1990 | 2193702 |
| molecular aspects of the virulence of pasteurella multocida. | molecules important to the virulence of all pasteurella multocida are little known, but much has been learned of isolates causing atrophic rhinitis of pigs. a protein toxin purified from p. multocida or from a recombinant escherichia coli reproduces atrophic rhinitis and leads to a reduction in weight gain; antitoxin is completely protective. toxin is important for p. multocida colonization, especially in the presence of the bordetella bronchiseptica cytotoxin. the p. multocida toxin binds rapid ... | 1990 | 2193704 |
| new diagnostic techniques: a review of the hap group of bacteria. | pasteurella haemolytica, pasteurella multocida, haemophilus somnus, haemophilus parasuis and actinobacillus pleuropneumoniae cause considerable economic loss in the cattle and swine industries. the literature relating to diagnostic procedures for these infections is reviewed. both conventional and newer techniques are appraised in relation to the identification and classification of isolates, and to serological testing. | 1990 | 2193708 |
| our understanding of the pasteurellaceae. | with the exception of a few consistent pathogens--pasteurella multocida strains of bovine hemorrhagic septicemia and fowl cholera, actinobacillus (haemophilus) pleuropneumoniae, haemophilus aegyptius and haemophilus paragallinarum--members of the family pasteurellaceae are commensal parasites on mucous membranes of vertebrate animals. many have pathogenic potential, which becomes manifest under conditions of immunodeficiency and stress. pathogenesis (except in porcine atrophic rhinitis) depends ... | 1990 | 2193710 |
| homogeneity of characteristics of pasteurella multocida isolated from turkeys and wildlife in california, 1985-88. | five hundred twenty isolates of pasteurella multocida, collected in california from september 1985 to november 1988, were characterized in the laboratory. characteristics examined included serotype, capsular type, biotype (subspecies), and possession of plasmid dna. three hundred thirty-three isolates recovered from turkeys dying from fowl cholera, 88 isolates from liver turkeys in flocks with fowl cholera outbreaks in the recent past, and 99 isolates from wildlife captured on fowl cholera-outbr ... | 1990 | 2196044 |