| molecular study on pasteurella multocida and mannheimia granulomatis from kenyan camels (camelus dromedarius). | outbreaks of a haemorrhagic septicaemia (hs) like disease causing large mortalities in camels (camelus dromedarius) in asia and in africa have been reported since 1890. yet the aetiology of this condition remains elusive. this study is the first to apply state of the art molecular methods to shed light on the nasopharyngeal carrier state of pasteurellaceae in camels. the study focused on hs causing pasteurella multocida capsular types b and e. other pasteurellaceae, implicated in common respirat ... | 2017 | 28830429 |
| pharmacokinetic-pharmacodynamic integration and modelling of oxytetracycline for the calf pathogens mannheimia haemolytica and pasteurella multocida. | a calf tissue cage model was used to study the pharmacokinetics (pk) and pharmacodynamics (pd) of oxytetracycline in serum, inflamed (exudate) and noninflamed (transudate) tissue cage fluids. after intramuscular administration, the pk was characterized by a long mean residence time of 28.3 hr. based on minimum inhibitory concentrations (mics) for six isolates each of mannheimia haemolytica and pasteurella multocida, measured in serum, integration of in vivo pk and in vitro pd data established ar ... | 2017 | 28736817 |
| surveillance for hemorrhagic septicemia in buffalo (bubalus bubalis) as an aid to range expansion of the javan rhinoceros (rhinoceros sondaicus) in ujung kulon national park, indonesia. | the javan rhinoceros (rhinoceros sondaicus) of ujung kulon national park (uknp) is the crown jewel of indonesia's rich natural history. the park lies on a peninsula surrounded by coastline and agriculture-dominated landscapes. the invasion of water buffalo (bubalus bubalis) into the park carries a substantial health risk to the javan rhinoceros and threatens plans to establish a new population outside of its only current range in uknp. hemorrhagic septicemia (hs), known locally as septicemia epi ... | 2017 | 28820298 |
| ompa protein sequence-based typing and virulence-associated gene profiles of pasteurella multocida isolates associated with bovine haemorrhagic septicaemia and porcine pneumonic pasteurellosis in thailand. | pasteurella multocida is a gram-negative bacterium that causes economically significant infections of a broad range of animal species. pneumonic and septicaemic pasteurellosis caused by this bacterium remain important problems in pigs, cattle, and water buffaloes in thailand. the aim of this study was to characterise the virulence-associated gene profiles and to develop an ompa molecular typing scheme for classifying 191 bovine and porcine isolates of p. multocida collected between 1989 and 2012 ... | 2017 | 28814302 |
| two novel cross‑protective antigens for bovine pasteurella multocida. | pasteurella multocida is an important pathogen that leads to a range of diseases that have severe economic consequences on cattle production. in order to develop an effective cross‑protective component vaccine, an immunoproteomics approach was used to analyze outer membrane proteins (omps) of the p. multocida serotype a, b and f strains. candidate antigen molecules from the whole genome were screened via linear trap quadrupole mass spectrometry and bioinformatics analysis, and the reactogenicity ... | 2017 | 28791368 |
| antimicrobial susceptibility, serotypes and genotypes of pasteurella multocida isolates associated with swine pneumonia in taiwan. | pasteurella multocida (pm) can cause progressive atrophic rhinitis and suppurative bronchopneumonia in pigs. the present study performed antimicrobial susceptibility testing and serotype and genotype identification on the 62 pm strains isolated from the lungs of diseased pigs with respiratory symptoms. antimicrobial susceptibility testing examined 13 antimicrobial agents (amoxicillin, cefazolin, doxycycline, flumequine, enrofloxacin, florfenicol, kanamycin, lincomycin, linco-spectin (lincomycin ... | 2017 | 28784694 |
| infection-stage adjusted dose of beta-lactams for parsimonious and efficient antibiotic treatments: a pasteurella multocida experimental pneumonia in mice. | in this study, the impact of infection stage on clinically and microbiologically efficacious doses and on antibiotic consumption was assessed during a naturally evolving infectious disease, using an original mouse model of pulmonary infection produced by air-borne contamination. when pasteurella multocida was administered as pathogenic agent to immunocompetent mice, 60% of the animals exhibited clinical symptoms of pneumonia 2 to 4 days after bacterial contamination of the lungs. two beta-lactam ... | 2017 | 28777819 |
| urinary tract infection with pasteurella multocida in a patient with cat exposure and abnormal urinary tract physiology: case report and literature review. | pasteurella multocida is a gram-negative organism that commonly colonizes the mouth of cats and dogs, and is known to cause infection in humans associated with animal bites or scratches. sites of infection other than skin and soft tissue are rare, but have been reported in patients with specific risk factors including anatomical abnormalities or immunosuppression. herein, we report a case of a symptomatic urinary tract infection caused by p. multocida in a 59 year old female who presented to the ... | 2017 | 28752063 |
| is submucosal fat injection effective in atrophic rhinitis? an experimental animal study. | atrophic rhinitis (ar) is a disease characterized by the extensive dilatation of the nasal cavity and atrophy of the mucosa, submucosa and bone tissue. its etiological factors are unknown. there is not a satisfying treatment yet and the treatment of the functional impairment in the atrophic cells is still subject to investigation. the objective of this study is to determine at the histopathological level the possible effects of the submucosal fat injection in an experimental model of ar. 12 albi ... | 2017 | 28707085 |
| genome wide host gene expression analysis in mice experimentally infected with pasteurella multocida. | pasteurella multocida causes acute septicemic and respiratory diseases, including haemorrhagic septicaemia, in cattle and buffalo with case fatality of 100%. in the present study, mice were infected with p. multocida (1.6 × 103 cfu, intraperitoneal) to evaluate host gene expression profile at early and late stages of infection using high throughput microarray transcriptome analyses. several differentially expressed genes (degs) at both the time points were identified in p.multocida infected sple ... | 2017 | 28704394 |
| pharmacokinetic/pharmacodynamic modeling of tulathromycin against pasteurella multocida in a porcine tissue cage model. | tulathromycin, a macrolide antibiotic, is used for the treatment of respiratory disease in cattle and swine. the aim of our study was to investigate the in vitro and ex vivo activities of tulathromycin in serum, (non-inflamed) transudate, and (inflamed) exudate against pasteurella multocida in piglets. the pharmacokinetics properties of tulathromycin were studied for serum, transudate, and exudate using a tissue cage model. in vitro antibiotic susceptibility of p. multocida and dynamic time-kill ... | 2017 | 28701951 |
| draft genome sequence of a virulent strain of pasteurella multocida isolated from alpaca. | pasteurella multocida is one of the most frequently isolated bacteria in acute pneumonia cases, being responsible for high mortality rates in peruvian young alpacas, with consequent social and economic costs. here we report the genome sequence of p. multocida strain unmsm, isolated from the lung of an alpaca diagnosed with pneumonia, in peru. the genome consists of 2,439,814 base pairs assembled into 82 contigs and 2,252 protein encoding genes, revealing the presence of known virulence-associate ... | 2017 | 28698737 |
| in vitro treatment of lipopolysaccharide increases the invasion of pasteurella multocida serotype b:2 into bovine aortic endothelial cell. | pasteurella multocida serotype b:2 causes haemorrhagic septicaemia (hs) in cattle and buffaloes. the invasion mechanism of the bacterium to invade the bloodstream is unclear. this study aims to characterize the effects of immunomodulatory molecules, namely dexamethasone and lipopolysaccharide treatments, on the invasion efficiency of p. multocida serotype b:2 towards bovine aortic endothelial cells (baecs) and the involvement of actin microfilament in the invasion mechanism. the results imply th ... | 2017 | 28693312 |
| transcriptomic analysis on responses of murine lungs to pasteurella multocida infection. | pasteurella multocida infection in cattle causes serious epidemic diseases and leads to great economic losses in livestock industry; however, little is known about the interaction between host and p. multocida in the lungs. to explore a fully insight into the host responses in the lungs during p. multocida infection, a mouse model of pasteurella pneumonia was established by intraperitoneal infection, and then transcriptomic analysis of infected lungs was performed. p. multocida localized and gre ... | 2017 | 28676843 |
| simultaneous streptococcus canis and pasteurella multocida peritonitis in a peritoneal dialysis patient. | | 2017 | 28676516 |
| antibakterielle in-vitro-wirksamkeit ätherischer öle gegen veterinärmedizinisch relevante keime klinischer isolate von hunden, katzen und pferden. | einleitung: ätherische öle sind die grundlage der aromatherapie. unter anderem wird ihnen eine antibakterielle wirkung zugeschrieben. in dieser studie sollte die in-vitro-wirksamkeit ätherischer öle gegen ein breites spektrum veterinärmedizinisch relevanter erreger getestet werden. methoden: die antibakterielle aktivität von 16 ätherischen ölen wurde mittels agardiffusionstest bestimmt. getestet wurden grampositive und gramnegative erreger, die aus klinischen isolaten von hunden, katzen und pfer ... | 2017 | 28654923 |
| interaction between pasteurella multocida b:2 and its derivatives with bovine aortic endothelial cell (baec). | pasteurella multocida b:2 causes bovine haemorrhagic septicaemia (hs), leading to rapid fatalities in cattle and buffaloes. an attenuated derivative of p. multocida b:2 gdh7, was previously constructed through mutation of the gdha gene and proved to be an effective live attenuated vaccine for hs. currently, only two potential live attenuated vaccine candidates for hs are being reported; p. multocida b:2 gdh7 and p. multocida b:2 jrmt12. this study primarily aims to investigate the potential of p ... | 2017 | 28629460 |
| integrated pharmacokinetic-pharmacodynamic (pk/pd) model to evaluate the in vivo antimicrobial activity of marbofloxacin against pasteurella multocida in piglets. | marbofloxacin is a veterinary fluoroquinolone with high activity against pasteurella multocida. we evaluated it's in vivo activity against p. multocida based on in vivo time-kill data in swine using a tissue-cage model. a series of dosages ranging from 0.15 to 2.5 mg/kg were administered intramuscularly after challenge with p. multocida type b, serotype 2. | 2017 | 28619095 |
| genome-wide analyses reveal genes subject to positive selection in pasteurella multocida. | pasteurella multocida, a gram-negative opportunistic pathogen, has led to a broad range of diseases in mammals and birds, including fowl cholera in poultry, pneumonia and atrophic rhinitis in swine and rabbit, hemorrhagic septicemia in cattle, and bite infections in humans. in order to better interpret the genetic diversity and adaptation evolution of this pathogen, seven genomes of p. multocida strains isolated from fowls, rabbit and pigs were determined by using high-throughput sequencing appr ... | 2017 | 28611758 |
| pasteurella multocida chest wall abscess without wounds. | | 2017 | 28560171 |
| pasteurella multocida ecthyma complicated by necrotizing fasciitis. | necrotizing fasciitis is a serious infection of the skin and soft tissues. pasteurella multocida is rarely reported to cause necrotizing fasciitis and is associated with high mortality. we describe a female patient with a past medical history of diabetes mellitus and myeloproliferative disorder presenting with bullae and erythema of the right forearm secondary to p. multocida infection after possible cat bite. despite adequate antibiotic coverage she developed necrotizing fasciitis diagnosed cli ... | 2017 | 28541874 |
| characterization of pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (mlst) and virulence-associated gene profile analysis and comparison with porcine isolates. | pasteurella multocida is a pathogen causing disease in a wide range of hosts including sheep and pigs. isolates from ovine pneumonia were characterized by mlst (multi-host and rirdc databases) and virulence-associated gene (vag) typing and compared with porcine isolates. ovine and porcine isolates did not share any sts as determined by both schemes and exhibited different vag profiles. with the multi-host database, sixteen sts were identified among 43 sheep isolates with two sts (st50 and st19) ... | 2017 | 28532799 |
| detection and genetic characterization of pasteurella multocida from alpaca (vicugna pacos) pneumonia cases. | pasteurella multocida is a common constituent of upper respiratory tract microbiota but is frequently isolated of alpaca lung tissues from pulmonary infections. despite its importance, very little is known about this bacteria at molecular level. in order to characterize p. multocida isolates, 24 isolates recovered from 46 mortal acute cases in young alpacas with suspected pneumonia were analyzed, using biochemical and molecular tests for capsule and lps typing, virulence factors detection, and e ... | 2017 | 28523388 |
| quantification of pasteurella multocida in experimentally infected pigs using a real-time pcr assay. | the aim of the study was to quantify pasteurella multocida in experimentally infected pigs using a new qpcr assay based on the soda gene and validated with 35 p. multocida strains, including strains isolated from pigs with pneumonia, clinically healthy pigs (nasal cavities), and human infections. the specificity of the test was verified with a collection of 60 strains of bacterial species other than p. multocida. the estimated detection threshold was 10 genome equivalents per microliter. the amp ... | 2017 | 28499212 |
| chronic intestinal pseudo-obstruction associated with enteric ganglionitis in a persian cat. | case summary a 7-year-old neutered male persian cat was presented for acute vomiting and inappetence. physical examination revealed severe abdominal distension. radiographs demonstrated pneumoperitoneum, megaoesophagus and generalised gaseous distension of the digestive tract. exploratory coeliotomy was performed, revealing markedly distended and thickened small and large intestines with no observable peristalsis. no intestinal perforation was present. bacteriological and cytological analysis of ... | 2017 | 28491428 |
| serological evidence of avian encephalomyelitis virus and pasteurella multocida infections in free-range indigenous chickens in southern mozambique. | a total of 398 serum samples from free-range indigenous chickens originating from four villages in southern mozambique were tested for the presence of avian encephalomyelitis virus (aev) and pasteurella multocida (pm) antibodies through commercial enzyme-linked immunosorbent assay (elisa) kits. aev and pm antibodies were detected in all villages surveyed. the proportion of positive samples was very high: 59.5% (95% confidence interval (ci) 51.7-67.7%) for aev and 71.5% (95% ci 67.7-77.3%) for pm ... | 2017 | 28474290 |
| pasteurella multocida meningoencephalitis in an immunocompetent adult with multiple cat scratches. | we present the case of a 49-year-old woman admitted to our acute medical unit with a 2-day history of fever, vomiting and confusion. the patient was alcohol dependent and had sustained several scratches from her pet cat, which her pet dog had licked. she deteriorated in the emergency department-developing high fever, worsening confusion and meningism. blood cultures were taken and broad spectrum antibiotics commenced prior to ct scanning and diagnostic lumbar puncture. blood cultures and csf 16s ... | 2017 | 28473359 |
| isolation, characterization, virulence and immunogenicity testing of field isolates of pasteurella multocida, staphylococcus aureus, and streptococcus agalactiae in laboratory settings. | the present study was designed to investigate isolation, characterization, virulence and immunogenicity testing of field isolates of pasteurella multocida, staphylococcus aureus, and streptococcus agalactiae in rabbits and mice. isolates of p. multocida, s. aureus and str. agalactiae recovered from field cases of hemorragic septicemia and mastitis were scrutinized for virulence/pathogenicity and immunogenicity. mouse ld50 of p. multocida showed that p. multocida isolate no.1 was more virulent th ... | 2017 | 28450213 |
| cryostored autologous skull bone for cranioplasty? a study on cranial bone flaps' viability and microbial contamination after deep-frozen storage at -80°c. | craniectomy is a life-saving procedure. subsequent cranioplasty with autologous skull bone has a bone resorption rate from 4% to 22.8% and an infection rate from 3.3% to 26%. there are concerns with their viability and the potential microbial contamination as they were explanted for a long period of time. eighteen cranial bone flaps stored at prince of wales hospital skull bone bank during the period from june 2011 to march 2016 were identified. ethics approval was obtained. bone chips and deep ... | 2017 | 28431953 |
| genome sequence of pasteurella multocida razi 0002 of avian origin. | we report here on the genome sequence of pasteurella multocida razi 0002 of avian origin, isolated in iran. the genome has a size of 2,289,036 bp, a g+c content of 40.3%, and is predicted to contain 2,079 coding sequences. | 2017 | 28408677 |
| hypercholesterolemia impairs oxytocin-induced uterine contractility in late pregnant mouse. | high cholesterol is known to negatively affect uterine contractility in ex vivo conditions. the aim of the present study was to reveal the effect of in vivo hypercholesterolemia on spontaneous and oxytocin-induced uterine contractility in late pregnant mouse uterus. female swiss albino mice were fed with high cholesterol (hc) diet (0.5% sodium cholate, 1.25% cholesterol and 15% fat) for 6 weeks and then throughout the gestation period after mating. on day 19 of gestation, serum cholesterol level ... | 2017 | 28400465 |
| bacterial pericarditis in a cat. | a 4-year-old male neutered domestic shorthair cat was presented to the oregon state university cardiology service for suspected pericardial effusion. cardiac tamponade was documented and pericardiocentesis yielded purulent fluid with cytologic results supportive of bacterial pericarditis. the microbial population consisted of pasteurella multocida, actinomyces canis, fusobacterium and bacteroides species. conservative management was elected consisting of intravenous antibiotic therapy with ampic ... | 2017 | 28491384 |
| pasteurella multocida peritonitis associated with a cat in a peritoneal dialysis patient using an automated cycler device. | pasteurella multocida (p. multocida) is an aerobic gram-negative coccobacillus, which is found as part of the natural oral flora of many animals, including most healthy cats and dogs. however, it can cause a variety of infections in humans, usually as a result of the patient being bitten or scratched by a cat or dog. there have been 22 reported cases of p. multocida peritonitis in patients undergoing peritoneal dialysis (pd). of these, 66.7 % occurred within 12 months of the initiation of pd. on ... | 2012 | 28509064 |
| pasteurella multocida infection in a dog bite wound: a case report. | | 1995 | 28769318 |
| development and standardization of an in-house indirect elisa for detection of duck antibody to fowl cholera. | serological tests, such as agglutination and indirect hemagglutination assay (iha), have been used to identify antibodies against pasteurella multocida in poultry sera, but none are highly sensitive. an enzyme-linked immunosorbent assays (elisa) has been used with varying degrees of success in attempts to monitor seroconversion in vaccinated poultry, but are not suitable for diagnosis. commercial elisa kits are available for chickens and turkeys, but not for ducks. the present study reports deve ... | 2017 | 28844720 |
| the myriad properties of pasteurella multocida lipopolysaccharide. | pasteurella multocida is a heterogeneous species that is a primary pathogen of many different vertebrates. this gram-negative bacterium can cause a range of diseases, including fowl cholera in birds, haemorrhagic septicaemia in ungulates, atrophic rhinitis in swine, and lower respiratory tract infections in cattle and pigs. one of the primary virulence factors of p. multocida is lipopolysaccharide (lps). recent work has shown that this crucial surface molecule shows significant structural variab ... | 2017 | 28825691 |
| a capsule/lipopolysaccharide/mlst genotype d/l6/st11 of pasteurella multocida is likely to be strongly associated with swine respiratory disease in china. | pasteurella multocida is a leading cause of respiratory disease in pigs worldwide. in this study, we determined the genetic characteristics of 115 p. multocida isolates from the lungs of pigs with respiratory disease in china in 2015 using capsular typing, lipopolysaccharide (lps) genotyping, and virulence genotyping based on the detection of virulence-associated genes. the results showed that the isolates belonged to three capsular types: a (49.6%), d (46.1%), and nontypable (4.3%); and two lps ... | 2017 | 28825122 |