| pasteurella multocida corneal ulcer following a baseball injury. | pasteurella multocida is an ubiquitous organism that can be isolated from a variety of animals and birds. it is an infrequent ocular pathogen but can cause infection as a result of injury or animal exposure. this article reports a case of p multocida corneal ulcer following a baseball injury. | 1989 | 2746683 |
| resistance to antimicrobial agents and prevalence of r plasmids in pasteurella multocida from turkeys. | one hundred fifty-three isolates of pasteurella multocida, representing the causative agent of 95% of all known outbreaks of fowl cholera occurring in california meat and breeder turkeys from august 1985 through february 1987, were examined for susceptibility to antimicrobial agents. of the 153 isolates, 6 were shown to be resistant to one or more antimicrobial agents. of the six resistant isolates, five contained r plasmids. all but one of the r plasmids were small (6 to 7 megadaltons) and nonc ... | 1989 | 2751281 |
| characteristics of fowl cholera outbreaks in turkeys in georgia in 1986. | information was gathered from 64 cases of fowl cholera (fc) in turkey flocks through diagnostic case records, flock records, and telephone and mail surveys. forty-five cases came from flocks of commercial turkeys, of which 15 were presented twice, and four came from mature breeder flocks. the prevalence of fc was 18.0% of commercial flocks and 14.7% of breeder flocks at risk. the average age at first diagnosis of fc was 90 days in commercial turkey flocks and 32 weeks 5 days in breeder flocks. a ... | 1989 | 2751551 |
| pathogenesis of fowl cholera: influence of encapsulation on the fate of pasteurella multocida after intravenous inoculation into turkeys. | the role of the capsule in the pathogenesis of fowl cholera was studied in turkeys. avian pasteurella multocida p-1059 was used in an encapsulated form, an enzymatically decapsulated form, and a mutant form lacking capsule-productivity (strain t-325). these forms were inoculated intravenously into normal or immune turkeys, and the numbers of viable bacteria in the blood, liver, and spleen were enumerated over a 120-minute period. both normal and immune birds rapidly removed all three forms of or ... | 1989 | 2751554 |
| solubilization of membrane-associated cross-protection factor(s) of pasteurella multocida. | pasteurella multocida harvested from the blood of turkeys dying of experimental fowl cholera were purified by centrifugation and lysed. the soluble and membrane-associated components of the bacteria were separated by centrifugation. nonionic (octylglucoside) and zwitterionic (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; chaps) detergents were tested for their abilities to solubilize the cross-protection factor(s) (cpf) from the membrane-associated component. protection studies in ... | 1989 | 2751557 |
| evaluation of pasteurella multocida mutants of low virulence. i. development and pathogenicity. | mutagenesis of the clemson university (cu) vaccine strain of pasteurella multocida with n-methyl-n-nitro-n-nitrosoguanidine resulted in temperature-sensitive mutants that grew at 37 c but not at 42 c. seven such mutants were evaluated for immunogenicity in turkeys. from these seven, only two, pm#1 and pm#3, provided turkeys with a level of protection against challenge with a virulent serotype 3 p. multocida strain (p-1059) comparable to the protection provided by the cu strain. intravenous (iv) ... | 1989 | 2751559 |
| evaluation of pasteurella multocida mutants of low virulence. ii. immunologic response of turkeys. | vaccination of turkeys by administering pasteurella multocida mutant pm#1 or pm#3, either by the oculo-nasal-oral method or in the drinking water, induced a level of protection comparable to vaccination with the clemson university (cu) strain or the m-9 vaccine. the level of protection was not altered when pm#1, pm#3, or the cu strain was grown in brain-heart infusion (bhi) broth or bhi agar. under extremely severe challenges, the cu strain provided a greater level of protection than pm#1, pm#3, ... | 1989 | 2751560 |
| time-resolved extrinsic fluorescence of aromatic-l-amino-acid decarboxylase. | the coenzyme-linked fluorescence of aromatic-l-amino-acid decarboxylase decays non-exponentially. the decay of both native and nabh4 reduced samples can only be fitted by two exponentials each roughly accounting for about half of the total fluorescence. denaturation of the reduced protein with 8 m urea makes the fluorescence decay mono-exponential, like that observed for the reference compound pyridoxamine-5-phosphate. an extra pyridoxyl moiety can be bound to the enzyme after incubation with ex ... | 1989 | 2752045 |
| [the "diseased" or "dead" guillemots (uria aalge), three-toed gulls (rissa tridactyla), silver gulls (larus argentatus) and laughing gulls (larus ridibundus) found in the area of the german bay, 1982-1985]. | between 1982 and 1985 the cadavers of 50 guillemots (uria aalge), 41 kittiwakes (rissa tridactyla), 26 herring gulls (larus argentatus) and 34 black-headed gulls (larus ridibundus) were examined pathological, bacteriological and virological. the probable cause of death was established. parasitosis were particularly prevalent in herring gulls (49%), where the main infection--as in black-headed gulls--was with cestoides. in kittiwakes and guillemots mainly spiruroideae were recorded. the commonest ... | 1989 | 2752934 |
| 131i-metaiodobenzylguanidine and 125i-iodoamphetamine. parameters of lung endothelial cell function and pulmonary vascular area. | radioiodinated metaiodobenzylguanidine (mibg) has been shown to be extracted in the lung by an active, sodium-dependent, saturable transport system similar to that which extracts norepinephrine. accordingly, first transit uptake of mibg has been investigated as a method for in vivo evaluation of pulmonary biogenic amine metabolism. issues that still must be resolved include the degree to which mibg extraction is affected by simple loss of available vascular surface (in distinction to changes in ... | 1989 | 2753053 |
| chlamydia trachomatis: a frequently overlooked public health menace. | genital chlamydia trachomatis infections affect 3-4 million americans each year and are considered to be the most prevalent of all sexually transmitted diseases in the united states. complications arising from chlamydial infections include scarring of the fallopian tubes, leading to ectopic pregnancy and infertility. because a significant proportion of chlamydial infections are asymptomatic, this disease is frequently overlooked. recent developments in low cost, rapid, antigen-detection assays h ... | 1989 | 2753693 |
| effects of hypoxia on metabolic rate of conscious adult cats during cold exposure. | oxygen consumption (vo2) and shivering movements were recorded in adult, conscious cats in a thermoneutral (24-27 degrees c) and in a cold (3-8 degrees c) environment during normoxia, hypoxia, or hyperoxia for 55 min. in the cold environment, vo2 correlated with shivering index (si) under conditions of normoxia or ambient hypoxia (fio2 = 0.12). during normoxia, vo2 was 63% higher in the cold than the thermoneutral environment. ambient hypoxia acutely reduced vo2 in cold and thermoneutral environ ... | 1989 | 2759960 |
| acute airsacculitis in turkeys inoculated with pasteurella multocida. | thirty female turkeys, inoculated into the caudal thoracic air sacs with pasteurella multocida were examined from 0 to 6 hours post-inoculation (pi). the air sac reacted rapidly and intensely with exudation of heterophils. circulating leukocyte and thrombocyte numbers remained normal except for an absolute lymphopenia by 6 hours pi. p. multocida was initially isolated from blood at 3 hours pi. total cell counts increased markedly in air sac lavage fluids by 1.5 hours pi and continued to increase ... | 1989 | 2763413 |
| resistance of some capsular serotype d strains of pasteurella multocida to rabbit polymorphonuclear neutrophil phagocytosis. | the mechanism of resistance of capsular type d strains of pasteurella multocida to killing by rabbit polymorphonuclear neutrophils (pmn) was studied using an in vitro assay that differentiates intra- from extracellular bacteria. two capsular type d strains (3761 and 3766), resistant to killing by rabbit pmn, and one type a strain (r1), susceptible to pmn destruction, were compared. after combining opsonized bacteria and pmn, the capsular type d strains 3761 and 3766 remained extracellular while ... | 1989 | 2763455 |
| antigenic relationship between the dermonecrotic toxins produced by pasteurella multocida type d and type a. | crude dermonecrotic toxins (dnt) were prepared from pasteurella multocida (p.m.) type d and type a strains isolated from pigs with atrophic rhinitis. rabbits were immunized with the dnt of p.m. type d. this serum neutralized the dnt of p.m. type a to the same degree as the homologous one both in vitro (cytopathogenicity for tissue culture cells) and in vivo (mouse lethality and dermonecrotic activity in guinea pig). | 1989 | 2763756 |
| in vitro mineralization of fetal rat parietal bones in defined serum-free medium: effect of beta-glycerol phosphate. | we have developed a bone organ culture system that mineralizes in vitro. fetal rat parietal bones (20 days old) were cultured in a chemically defined serum-free medium containing physiological 3 mm phosphate. during 5 days in culture, calcium content increased from 26 to 55 micrograms and dry weight increased from 137 to 194 micrograms. after 2 days in vivo, the calcium content of the parietal bone showed a comparable increase to 49 micrograms and dry weight increased to 183 micrograms. during c ... | 1989 | 2763870 |
| pasteurella multocida and bordetella bronchiseptica in atrophic rhinitis and pneumonia in swine. | a total of 163 pigs from nine farrow-to-finish herds representing various levels of atrophic rhinitis (ar) were selected for postslaughter examination of ar and pneumonia. nasal swabs and lungs were cultured for detection of bordetella bronchiseptica and pasteurella multocida. seventy-three pigs were examined at eight weeks of age and 90 contemporaries at six months of age. mean ar scores were 1.21 and 1.11 for the eight week and six month old pigs, respectively (0 = normal, 3 = severe). in indi ... | 1989 | 2766151 |
| isolation of neisseria canis in mixed culture from a patient after a cat bite. | we report a case of identification of neisseria canis in a human patient after a cat bite. this organism was isolated from a mixed culture together with pasteurella multocida and eikenella corrodens. it is the second case report of a human infection. | 1989 | 2768456 |
| use of an rrna probe and restriction endonuclease analysis to fingerprint pasteurella multocida isolated from turkeys and wildlife. | twenty-five isolates of the bacterium pasteurella multocida were characterized (fingerprinted) phenotypically and genotypically in order to compare the abilities of various techniques to differentiate strains for epidemiologic studies of fowl cholera. isolates were obtained over a 16-month period from turkeys dying from fowl cholera (six outbreak flocks) and from wildlife captured on premises with a history of the disease. the characteristics compared included (i) serotype, (ii) subspecies, (iii ... | 1989 | 2768471 |
| interactions between bordetella bronchiseptica and toxigenic pasteurella multocida in atrophic rhinitis of pigs. | three strains of bordetella bronchiseptica were compared for their ability to assist colonisation of the nasal cavity of gnotobiotic pigs by toxigenic pasteurella multocida. toxigenic p multocida (counted in nasal washings) colonised the cavity in large numbers in pigs previously infected with a cytotoxic phase i strain of b bronchiseptica (b58), whereas it colonised only in small numbers in those previously infected with b65, a phenotypic phase iii variant of b58. toxigenic p multocida colonise ... | 1989 | 2772406 |
| sympathetic uveitis after giant tear repair. | a 33-year-old hispanic man developed sympathetic ophthalmia 4.5 months after vitrectomy and repeat scleral buckling for a giant retinal tear in an aphakic previously traumatized eye. the patient had gray-white, subretinal nodules with characteristic changes of sympathetic uveitis detected by fluorescein angiography. the fluorescein angiographic and clinical findings revealed a dramatic response to steroid treatment: visual acuity returned to the previous 20/20 level within 5 weeks of therapy. | 1989 | 2772419 |
| in vitro antimicrobial activity of sulfonamides against some porcine pathogens. | the minimal inhibitory concentrations (mic) of sulfonamides were determined against bordetella bronchiseptica (n = 10), pasteurella multocida (n = 10), haemophilus pleuropneumoniae (n = 20), and streptococcus suis (n = 10) strains isolated from pigs with atrophic rhinitis, pneumonia, or meningitis. sulfonamides tested in an agar dilution method were sulfachloropyridazine, sulfadiazine, sulfadimethoxine, sulfamethazine, sulfadoxine, sulfisoxazole, sulfamerazine, sulfamethoxazole, sulfamethoxypyri ... | 1989 | 2774319 |
| relationship between anti-pasteurella multocida antibody titers after cu vaccination and survival after challenge. | the relationship between serum anti-pasteurella multocida antibodies and survival rates after challenge was determined in turkeys vaccinated one or more times with the live avirulent clemson university (cu) vaccine and then challenged with a virulent isolate (9481) of p. multocida in the drinking water. a microtiter agglutination test for assaying anti-p. multocida serum antibodies demonstrated a highly significant (p less than 0.001) correlation between the serum antibody titer 1 week after the ... | 1989 | 2775097 |
| ability of the lapicque and blair strength-duration curves to fit experimentally obtained data from the dog heart. | the objective of this study was to determine the ability of the empirical lapicque and theoretically derived blair expressions for excitation to fit experimentally obtained threshold current values to evoke a ventricular extrasystole using rectangular-wave stimuli applied to the dog heart. the data points were fitted to both expressions and the ability of each to predict the measured values was determined. the levenberg-marquardt (l-m) algorithm was used to fit the lapicque and blair expressions ... | 1989 | 2777286 |
| changes in the relative concentrations of surfactant phospholipids in young pigs with experimental pneumonia. | lung lavage fluids of 23 young pigs were investigated prior to and after experimental infection with pasteurella multocida. comparison of the phospholipid patterns showed an increase in the relative concentration of phosphatidylinositol and a decrease in that of phosphatidylglycerol in the diseased animals. the phosphatidylcholine-to-phosphatidylinositol and phosphatidylglycerol-to-phosphatidylethanolamine ratios were used as parameters to characterize the changing patterns. the reductions in th ... | 1989 | 2781896 |
| human t lymphocytes possess a limited in vitro life span. | the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ... | 1989 | 2786475 |
| the screening of propofol in mhs swine. | this study investigated the use of propofol in swine genetically susceptible to malignant hyperthermia (mh). thirteen animals were exposed to 2% halothane in inspired air, a propofol infusion of 12 mg.kg-1.hr-1 for up to 45 min, or a combination of both. when mh was triggered the animals were treated with dantrolene. mean onset time of malignant hyperthermia on exposure to halothane alone was 7.2 min +/- 3.0 min and onset with propofol and halothane was 5.0 +/- 2.5 min. in no instance did propof ... | 1989 | 2786680 |
| trimethoprim/sulfamethoxazole therapy of pasteurella multocida infection. | | 1989 | 2788197 |
| dendrotoxin-binding brain membrane protein displays a k+ channel activity that is stimulated by both camp-dependent and endogenous phosphorylations. | the purified protein that binds the k+ channel ligands dendrotoxin i and mast cell degranulating peptide can be phosphorylated by camp-dependent protein kinase and by an endogenous protein kinase, which may be a specific k+ channel kinase. phosphorylations take place on the toxin-binding subunit, a polypeptide of 76-80 kda. phosphorylation by both kinases leads to activation of the reconstituted dendrotoxin-sensitive k+ channel. | 1989 | 2790006 |
| estradiol-stimulated increases in uterine eosinophils and nuclear type ii estrogen-binding sites are prevented by pertussis toxin. | previous studies from several laboratories have demonstrated that estradiol treatment resulted in an increase in nuclear type ii binding sites. our previous data suggest that this increase was due to the estradiol-stimulated influx of circulating eosinophils. therefore, we suggested that the uterine nuclear type ii estrogen-binding sites were not of uterine origin. in this report we present further evidence to support this hypothesis. treatment of immature rats with estradiol resulted in the sti ... | 1989 | 2792007 |
| endophthalmitis due to pasteurella multocida and cdc ef-4. | | 1989 | 2794571 |
| intravascular thrombosis in skeletal muscle microcirculation after ischemia. | this study was undertaken to elucidate the role of thrombosis in blood vessels less than 0.1 mm in diameter in skeletal muscle after an ischemic episode. capillaries were examined after normothermic ischemia using intravital video microscopy of the cremaster muscle of an anesthetized rat. histologic sections of the cremaster muscle and silicone rubber intravascular casts were also analyzed. our model demonstrates that, after 3 hours of warm ischemia, up to two-thirds of capillaries are clotted, ... | 1989 | 2796711 |
| integrating values education into an allied health curriculum; part ii. | values education involves the explicit attempt to teach individuals about values and the process of valuing. an overview of popular approaches to values education includes inculcation, value clarification, moral development, and value analysis. each approach is presented by detailing the purpose, teaching methods, and an instructional model. methods of incorporating these approaches into an allied health curriculum are offered along with sample student learning activities. | 1989 | 2798842 |
| pharmacokinetics of single doses of cefoperazone given by the intravenous and intramuscular routes to unweaned calves. | cefoperazone pharmacokinetics were studied in unweaned calves. the antibiotic was administered to 10 calves intravenously, to eight calves intramuscularly at 20 mg kg-1 and to 10 calves intramuscularly at 20 mg kg-1 together with probenecid at 40 mg kg-1. serum concentration versus time data were analysed by non-compartmental methods based on the statistical moment theory. the intravenous data were also fitted by a linear, open two-compartment model. the terminal halflife of cefoperazone was 127 ... | 1989 | 2799072 |
| experimental pasteurella multocida pneumonia in calves. | pasteurella multocida was isolated from the lungs of calves that died on a farm in the south of england. this organism was inoculated experimentally into 13 calves by the intratracheal route: in all but two of the calves mild clinical disease resulted and at necropsy, three or four days later, pneumonic consolidation involving up to 22 per cent of the lung was observed. p multocida was isolated from all but two of the lungs. of two calves inoculated intravenously with p multocida, one showed mil ... | 1989 | 2799074 |
| [incidence of coxiella burnetti and chlamydia psittaci in cattle in southern bohemia]. | in 1986 and 1987 in six districts and 22 localities of the southern bohemian region, and in one district and one locality of the western bohemian region the total of 747 head of cattle was examined by a microagglutination method (mar) and complement fixation method (kfr) for antibodies to coxiella burnetii and chlamydia psittaci. the positivity of c. burnetii detection was 44.4% in mar and 16.5% in kfr. the positivity of chl. psittaci detection was 7.1%. the widespread incidence and highly posit ... | 1989 | 2800248 |
| [incidence of coxiella burnetti and chlamydia psittaci in cattle in southern bohemia]. | in 1986 and 1987 in six districts and 22 localities of the southern bohemian region, and in one district and one locality of the western bohemian region the total of 747 head of cattle was examined by a microagglutination method (mar) and complement fixation method (kfr) for antibodies to coxiella burnetii and chlamydia psittaci. the positivity of c. burnetii detection was 44.4% in mar and 16.5% in kfr. the positivity of chl. psittaci detection was 7.1%. the widespread incidence and highly posit ... | 1989 | 2800248 |
| [microbiological dehydrogenation of intermediate of fluocinanide acetate]. | both compound i and ii are intermediates in fluocinanide acetate synthesis. i could be dehydrogenated to ii in 62% approximately 63% yields by arthrobactor simplex no. a-1, which was selected in our laboratory. when concentration of i was 0.1%, it was transformed so fast that ii could not be accumulated. when concentration was increased to 0.2%, four intermediates iv, v, vi and vii were formed in addition to a little amount of product ii. when concentration of substrate i was increased to 0.5% a ... | 1989 | 2801119 |
| atrophic rhinitis in new zealand white rabbits infected with pasteurella multocida. | atrophic rhinitis was detected in new zealand white rabbits when upper respiratory tract disease was evaluated during a vaccine field trial for the prevention of pasteurellosis. of 52 adult rabbits euthanatized and necropsied, 26 (50%) had evidence of turbinate atrophy. atrophy was detected in 77% of rabbits with pasteurella multocida infection only, 71% of rabbits with concurrent p multocida and bordetella bronchiseptica infections, and 6% of rabbits with b bronchiseptica infection only. grossl ... | 1989 | 2802315 |
| [yearly dynamics of arylsulfatase activity in the myocardium in intact rabbits]. | | 1989 | 2804317 |
| [yearly dynamics of arylsulfatase activity in the myocardium in intact rabbits]. | | 1989 | 2804317 |
| [electrophysiological analysis of the interaction of the hypothalamic preoptic area and the nonspecific system of the thalamus in the rabbit]. | | 1989 | 2806656 |
| non-organ specific & organ-specific auto-antibodies in hepatitis-b virus associated glomerulonephropathies. | eighty eight patients of glomerulonephropathies (hbsag positive 67; hbsag negative 21) and 88 matched and healthy controls were screened for non-organ specific autoantibodies-ana, ama, asma and apca by indirect immunofluorescent technique. the 2.3 per cent positivity in the test group and the 8 per cent positivity in the control group did not suggest the involvement of hepatitis-b virus (hbv), as an influencing or associated agent. when 48 patients with glomerulonephropathies and 23 controls wer ... | 1989 | 2807426 |
| [cation transport and calcium-induced hemolysis in the erythrocytes of patients with hypertension and in spontaneously hypertensive rats (comparative analysis)]. | a comparison was made of the physicochemical characteristics of erythrocyte membranes in two strains of spontaneously hypertensive rats, namely shr and mhs and in patients with essential hypertension (eh). the authors confirmed the results of the previously reported studies that na+/li+/h+ countertransport rates were higher in the erythrocytes of patients with eh. the high rate of l1+ sodium-independent efflux hindered identification of na+/h+ turnover in the rat erythrocytes. na+/h+ turnover ra ... | 1989 | 2811049 |
| infection with and antibody response to pasteurella multocida and bordetella bronchiseptica in immature rabbits. | at 2, 4, 6, 8 and 10 weeks of age rabbits were cultured for pasteurella multocida and bordetella bronchiseptica from the paranasal sinuses, trachea, middle ears, lungs and liver. sera were tested for antibodies (igg) against p. multocida and b. bronchiseptica. pasteurella multocida was isolated from all ages of rabbits, and b. bronchiseptica was isolated from rabbits 4 to 10 weeks old. the sinuses were colonized most often, followed by the trachea, middle ears and lungs. no bacteria were isolate ... | 1989 | 2811279 |
| [the fatty acid composition of haemophilus and pasteurella multocida cells as a phylogenetic marker]. | when grown on meat-peptone agar with heated blood, different haemophilus species (h. influenzae, h. parahaemolyticus, h. parasuis, h. pleuropneumoniae), including different h. influenzae serovars (a, b, c, d, e, f), and pasteurella multocida have identical fatty acid composition, characterized by the prevalence of fatty acids with 16 carbon atoms, constituting about 70% and more of the total number of fatty acids, and a low level of fatty acids with 18 carbon atoms. p. multocida strains cultivat ... | 1989 | 2816180 |
| use of nude (athymic) mice for the study of hypertrophic scars and keloids: vascular continuity between mouse and implants. | hypertrophic scars and keloids appear to be unique to humans since animals are not known to form these lesions. therefore, in an effort to develop an experimental model for their study, implants of these human lesions were made in nude (athymic) mice (nu/nu) in suprascapular subcutaneous pockets. the implants were recovered from 2 to 246 days. by histological and fine structural parameters all implants remained viable and their morphological character was maintained. selected mice were injected ... | 1989 | 2817436 |
| toxigenic type d pasteurella multocida in new south wales pig herds--prevalence and factors associated with infection. | between march and july 1987, a study was undertaken to determine the prevalence of and factors associated with toxigenic type d pasteurella multocida infection in new south wales pig herds. toxigenic type d p. multocida was isolated from the nasal cavities of pigs in one (2%) of 50 randomly selected herds. toxigenic isolates were also recovered from 2 (8%) of a separate group of 25 herds that had purchased pigs from a known infected piggery in south australia (herd sa). snout abnormalities were ... | 1989 | 2818358 |
| expression of prokaryotic genes after transfection of x-irradiated plasmids into primate cells. | expression of the prokaryotic gene for chloramphenicol acetyltransferase (ec 2.3.1.28) (cat) in primate cells transfected with x-irradiated plasmid psv2cat was determined in transient expression assays. cat expression did not depend upon the presence of supercoiled plasmids, but relaxed circular forms were essential. x-ray conversion of relaxed circles to linear forms paralleled the loss of cat expression, with identical d0's in the first part of dose-response curves. x-ray-induced loss of super ... | 1987 | 2819935 |
| reaction of tryptophanyl-trna synthetase from beef pancreas with periodate-oxidized atp. | tryptophanyl-trna synthetase from beef pancreas reacts with periodate-oxidized atp according to biphasic kinetics. a rapid phase involves two groups of the protein, presumably lysine side-chains. the slow phase corresponds to the reaction of a larger number of groups. the time-course of the partial losses of the atp-ppi isotopic exchange and of the aminoacylation activities of the enzyme follow the labelling of the two fast-reacting groups. however, the ability of the enzyme to form a bis(trypto ... | 1987 | 2820730 |
| structural and functional analysis of a cloned segment of escherichia coli dna that specifies proteins of a c4 pathway of serine biosynthesis. | the plasmid pdr121 is a pbr322 derivative that contains a 3.7-kilobase-pair ecori fragment of dna from the 81.2-min region of the escherichia coli chromosome. the genomic insert encodes threonine dehydrogenase and at least one other protein. several physical and kinetic properties of threonine dehydrogenase, overproduced in cells harboring pdr121, are identical to those of pure threonine dehydrogenase from a haploid mutant of e. coli k-12 that produces this enzyme constitutively. tester strains ... | 1987 | 2820941 |
| structural and functional analysis of a cloned segment of escherichia coli dna that specifies proteins of a c4 pathway of serine biosynthesis. | the plasmid pdr121 is a pbr322 derivative that contains a 3.7-kilobase-pair ecori fragment of dna from the 81.2-min region of the escherichia coli chromosome. the genomic insert encodes threonine dehydrogenase and at least one other protein. several physical and kinetic properties of threonine dehydrogenase, overproduced in cells harboring pdr121, are identical to those of pure threonine dehydrogenase from a haploid mutant of e. coli k-12 that produces this enzyme constitutively. tester strains ... | 1987 | 2820941 |
| a binding site for [3h]glipizide in the rat cerebral cortex. | [3h]glipizide, a 2nd generation hypoglycemic sulfonylurea, binds specifically to rat cerebral cortex membranes in a time- and temperature-dependent way. the binding is saturable and reversible. the maximal binding capacity is 110 fmol/mg protein and the dissociation constant 1.5 nm. the binding site was destroyed by proteolytic and lipolytic enzymes suggesting a lipoprotein nature. active analogs of sulfonylureas are characterized by ic50 values in the cerebral cortex which parallel their insuli ... | 1987 | 2822437 |
| cervical mosaic and an integrated pathophysiological approach to early cervical neoplasia. | the increasing concern for the early detection and treatment of cervical intraepithelial neoplasia (cin) set up the basis for this study, which also considers some epidemiological factors which seem to be related to the genesis of an abnormal uterine cervical colposcopic pattern, the mosaic pattern. we have observed that women with a mosaic pattern show an increasing frequency of premalignant and/or malignant histologic changes up to 40 years of age which then falls abruptly. sterility and hirsu ... | 1987 | 2824702 |
| [angioscintigraphy in benign tumors of the uterus and adnexa uteri]. | angioscintigraphy was used for examination of 128 patients, of them 91 had tumors of the genital organs, 22 inflammatory adnexal processes and 15 various extragenital diseases. statistical scintigraphy was performed 5-40 min. after rp intravenous injection at 175-555 mbq. rp injection was followed by dynamic studies, the rate of recording being 1 frame per min. an anterioposterior direct projection with alignment of a gamma-chamber detector over the small pelvic area was used. 99mtc-human serum ... | 1987 | 2824965 |
| effects of tilomisole, indomethacin and levamisole on regulation of epstein barr virus-induced b cell proliferation by peripheral blood mononuclear cells from normal individuals and patients with rheumatoid arthritis. | when activated in autologous mixed leukocyte reactions (auto-mlr) in vitro, t cells from normal individuals produce a suppressor factor(s) which inhibits the epstein-barr virus (ebv)-induced proliferation of normal b cells. in contrast, t cells from patients with rheumatoid arthritis (ra) are deficient in their ability to generate this suppressor factor in auto-mlr. addition of tilomisole (wy-18,251; 33(p-chlorophenyl)thiazolo[3,2-a]benzimidazole-2-acetic acid) to the auto-mlr (0.1-100 microgram ... | 1987 | 2825478 |
| the effects of oxygen free radical scavengers on skeletal muscle ischemia and reperfusion injury. | | 1987 | 2826082 |
| ngf receptor immunoreactivity in rat brain: topographic distribution and response to entorhinal ablation. | nerve growth factor receptor (ngfr) immunoreactivity was distributed in neuronal cell bodies, axons and/or dendrites of cholinergic and non-cholinergic structures of the rat brain. the dentate gyrus of normal animals showed immunostained fibers mainly in the supragranular band, whereas following entorhinal ablation a greater density of immunostained fibers appeared in the outer molecular layer and a wider clear zone was apparent in the inner molecular layer. the ngfr-positive fibers followed the ... | 1987 | 2827075 |
| cloning, sequence, and expression of the drosophila camp-dependent protein kinase catalytic subunit gene. | genomic dna containing the protein coding region for drosophila camp-dependent protein kinase catalytic subunit has been cloned and sequenced. the probe used to detect and isolate the gene fragment was constructed from two partially complementary synthetic oligonucleotides and contains 60 base pairs that encode (using drosophila codon preferences) amino acids 195-214 of the beef heart catalytic subunit. in reduced stringency hybridization conditions, the probe recognizes two target sites in fly ... | 1988 | 2828348 |
| cloning, sequence, and expression of the drosophila camp-dependent protein kinase catalytic subunit gene. | genomic dna containing the protein coding region for drosophila camp-dependent protein kinase catalytic subunit has been cloned and sequenced. the probe used to detect and isolate the gene fragment was constructed from two partially complementary synthetic oligonucleotides and contains 60 base pairs that encode (using drosophila codon preferences) amino acids 195-214 of the beef heart catalytic subunit. in reduced stringency hybridization conditions, the probe recognizes two target sites in fly ... | 1988 | 2828348 |
| in vivo and in vitro models of demyelinating disease, xxi: relationship between differentiation of rat oligodendrocytes and control of jhmv replication. | | 1987 | 2829533 |
| characterization of temperature-sensitive mutants mouse hepatitis virus type 3. | | 1987 | 2829537 |
| [discordance between the short and standard metyrapone tests in isolated corticotropin insufficiency]. | a 71-year-old man presented with clinical and common biological features of secondary adrenal insufficiency. basal acth level was in the normal range. as it has been previously reported in acth deficiency, cortisol response to acth-test was normal. a single-dose metyrapone test, performed to confirm the diagnosis of adrenal insufficiency, gave unexpectedly normal results. however 11-desoxycortisol (compound s) response to standard-metyrapone test was subnormal ascertaining the diagnosis of acth- ... | 1987 | 2833152 |
| immunoblotting with polyclonal and monoclonal antibody to avian myeloblastosis protein p27: studies of liver proteins in chickens with erythroblastosis. | an antigen detected by complement fixation with polyclonal antibody to avian myeloblastosis virus (amv) antigen p27, appears in the livers of chickens inoculated with avian erythroblastosis virus (aev). it can be demonstrated at the 30,000 dalton (30k) molecular weight level by western immunoblotting of electropherograms of aev infected liver extracts. the 30k protein reacted strongly with this polyclonal antibody but only weakly with a monoclonal antibody to the same viral antigen and possible ... | 1987 | 2836998 |
| mk-801, a proposed noncompetitive antagonist of excitatory amino acid neurotransmission, produces phencyclidine-like behavioral effects in pigeons, rats and rhesus monkeys. | the behavioral effects of mk-801 [(+)-5-methyl-10,11-dihydroxy-5h-dibenzo(a,d)cyclohepten-5,10-imin e], a proposed noncompetitive n-methyl-d-aspartate (nmda) antagonist, were compared to those of phencyclidine (pcp). in pigeons, mk-801 produced pcp-like catalepsy (i.e., loss of righting without eye closure and without muscle relaxation) and pcp-like discriminative stimulus effects. in rats, mk-801 produced pcp-like behavior (i.e., locomotion, sniffing, swaying and falling). in rhesus monkeys, li ... | 1988 | 2838610 |
| [discovery of a new rotavirus in the feces of patients with acute diarrhea]. | | 1988 | 2842016 |
| detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration. | the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ... | 1988 | 2844405 |
| heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord. | messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ... | 1988 | 2845580 |
| comparative in vitro activity of the new oral macrolide azithromycin. | the in vitro activity of the new oral macrolide azithromycin was compared with that of erythromycin against gram-positive and gram-negative aerobic and anaerobic bacteria. ninety percent of hemolytic streptococci groups a and b, and streptococcus pneumoniae were inhibited by 0.5 microgram/ml. activity of azithromycin was similar to that of erythromycin; erythromycin-resistant staphylococci and streptococci were not inhibited. azithromycin was more active than erythromycin against haemophilus inf ... | 1988 | 2846300 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| suppression of sv40-promoted gene expression by differentiation of preadipose cells. | when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ... | 1988 | 2849579 |
| multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mrna. | we have determined the effects of a number of mutations in the small-t antigen mrna intron on the alternative splicing pattern of the simian virus 40 early transcript. expansion of the distance separating the small-t pre-mrna lariat branch point and the shared large t-small t 3' splice site from 18 to 29 nucleotides (nt) resulted in a relative enhancement of small-t splicing in vivo. this finding, coupled with the observation that large-t pre-rna splicing in vitro was not affected by this expans ... | 1988 | 2851720 |
| [changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals]. | | 1988 | 2851783 |
| two inhibitory postsynaptic potentials, and gabaa and gabab receptor-mediated responses in neocortex of rat and cat. | 1. pyramidal neurones from layers ii and iii of the rat primary somatosensory cortex and cat primary visual cortex were studied in vitro. inhibitory postsynaptic potentials (ipsps) and responses to exogenously applied gamma-aminobutyric acid (gaba) and its analogue baclofen were characterized. the results from rats and cats were very similar. 2. single electrical stimuli to deep cortical layers evoked a sequence of psps in the resting neurone: (a) an initial, brief excitation (epsp), (b) a short ... | 1988 | 2855437 |
| two inhibitory postsynaptic potentials, and gabaa and gabab receptor-mediated responses in neocortex of rat and cat. | 1. pyramidal neurones from layers ii and iii of the rat primary somatosensory cortex and cat primary visual cortex were studied in vitro. inhibitory postsynaptic potentials (ipsps) and responses to exogenously applied gamma-aminobutyric acid (gaba) and its analogue baclofen were characterized. the results from rats and cats were very similar. 2. single electrical stimuli to deep cortical layers evoked a sequence of psps in the resting neurone: (a) an initial, brief excitation (epsp), (b) a short ... | 1988 | 2855437 |
| a role for amniotic epithelium in control of human parturition. | a peroxidase anti-peroxidase staining technique was used to determine the presence of pregnancy-associated prostaglandin synthetase inhibitor (papsi) in human amniotic epithelium from 20 pregnant women at 36-40 weeks' gestation. papsi was present in amniotic epithelial cells in 12 of 15 women before the onset of labour. no papsi was found in amniotic epithelium from 5 women in clinically established labour. these findings indicate that the amnion is a source of this endogenous prostaglandin synt ... | 1985 | 2860289 |
| clostridial infection of renal cell carcinoma. | we report a case of a large gas-filled clostridial abscess in a previously unrecognized renal cell carcinoma. neoplastic tissue has a nonhomogeneous blood supply, creating areas of hypoxia and reduced glucose concentrations, which lead to tumor necrosis and an environment conducive to the growth of anaerobic organisms. anaerobic infection should be considered in any patient with carcinoma and fever. conversely, abscess transformation of a tumor can be the explanation for what otherwise seems to ... | 1986 | 2868130 |
| pharmacology of the ruminant gastroduodenal junction. | | 1985 | 2869150 |
| [effects of alpha-methyldopa on systolic pressure and activities of tyrosine hydroxylase, dopamine-beta-hydroxylase and phenethanolamine-n-methyl-transferase in nervous system of spontaneously hypertensive rats]. | | 1985 | 2876577 |
| absence of age-related changes in the binding of the beta adrenergic antagonist 125i-iodohydroxybenzylpindolol in rat heart. | the effect of age on the density and the affinity of beta adrenergic receptors was determined in the hearts of fischer 344 rats at three ages, 6, 12, and 24 months old. the binding of the beta adrenergic antagonist 125i-iodohydroxybenzylpindolol (ihyp), was used to quantitate and characterize cardiac beta adrenergic receptors. the maximal number of binding sites (bmax = f moles/mg of protein) were 26.3 +/- 2.5, 25.4 +/- 0.99, and 24.5 +/- 2.4 and the dissociation constants (kd = nm) were 0.166 + ... | 1987 | 2888126 |
| restriction enzyme maps of the macropodid herpesvirus 2 genome. | the dna of macropodid herpesvirus 2 (dorcopsis wallaby herpesvirus) was analysed using restriction enzymes and molecular cloning techniques. sets of ecori and sali clones were prepared which represented approximately 85 per cent of the genome, and these clones were used to map the dna by double-digestion and hybridization experiments. data on uncloned regions were obtained by analysing fragments excised from agarose gels, and terminal fragments were identified by exonuclease iii digestion. the g ... | 1987 | 2889444 |
| presence of pili in pasteurella multocida strains associated with atrophic rhinitis. | | 1988 | 2896409 |
| effect of n-n'-diphenyl-p-phenylenediamine pretreatment on urinary enzyme excretion in cisplatin nephrotoxicity in rats. | two days after cisplatin was injected into rats, urinary n-acetyl-beta-d-glucosaminidase (nag) and gamma-glutamyltranspeptidase (gamma-gtp) activities increased. the urinary excretion of nag continued to rise until 4 days after the injection of cisplatin, the last day examined. however, the increase in urinary gamma-gtp excretion which lasted for 2 days returned to its control level 4 days after cisplatin injection. the alkaline phosphatase activity in urine was unaffected by cisplatin injection ... | 1988 | 2896807 |
| hypothermic response produced by manassantin a, a novel neuroleptic agent. | manassantin a (mns-a), a novel neolignoid, neutral compound shown to possess neuroleptic properties, causes hypothermic response in male and female mice of cd-1 strain when administered by the intra-cerebroventricular (icv), (0.1, 1.0, 3.2, 10 micrograms/mouse), intraperitoneal (ip), (0.1, 0.32, 1.0, 3.2 mg/kg) and oral (0.5, 1.6, 5.0, 16 mg/kg) routes. the hypothermia was found to be dose and time dependent, the maximum decrease of temperature being observed by the icv route (p less than 0.001) ... | 1988 | 2898718 |
| expression of pili and capsule by the avian strain p-1059 of pasteurella multocida. | the avian strain p-1059 of pasteurella multocida was grown on blood agar (ba), on dextrose-starch agar (dsa), or in heddleston's hydrogen sulfide test broth. cells were examined for the presence of pili using electron microscopy after staining with phosphotungstic acid, and they were examined for capsule after ruthenium red staining. pili were found on the capsulated iridescent type, p-1059i, and on two non-capsulated variants, the blue, p-1059b, and the gray, p-1059g. many cells grown on ba wer ... | 1988 | 2900000 |
| inhibitory effect of glycyrrhetinic acid derivatives on arachidonic acid-induced mouse ear oedema. | the inhibitory effects of glycyrrhetinic acid and its derivatives were examined on arachidonic acid (aa)-induced ear oedema in mice. of the compounds, dihemiphthalate derivatives of 18 beta-olean-12-ene-3 beta, 30-diol (iid, iid'), 18 beta-olean-9(11)12-diene-3 beta, 30-diol (iiia, iiia') and olean-11, 13(18)-diene-3 beta, 30-diol (iva, iva') showed a strong inhibition of ear oedema on both tropical (id50, 1.9, 2.8 and 1.7 mg/ear, respectively) and oral (id50, 90, 130 and 88 mg kg-1, respectivel ... | 1988 | 2900308 |
| alpha adrenoceptor subtypes and receptor reserve in human versus canine saphenous vein: sensitivity to blockade by nitroglycerin. | the purpose of this investigation was to determine the subtypes of alpha adrenoceptors present in human saphenous vein and to determine if there is a large receptor reserve for phenylephrine as has been demonstrated in canine saphenous vein. the subtypes of alpha adrenoceptors found in isolated human saphenous vein were determined using selective alpha-1 and alpha-2 adrenoceptor agonists and antagonists. prazosin, a selective alpha-1 antagonist, produced a parallel shift of the concentration res ... | 1988 | 2905005 |
| effects of chronic peritoneal dialysis on thyroid function tests. | peritoneal dialysis is associated with large losses of protein. in order to quantify thyroid hormone excretion in the dialysate and to examine the possibility that peritoneal dialysis may result in clinical hypothyroidism, nine endstage renal disease (esrd) patients undergoing either continuous ambulatory peritoneal dialysis (capd) or chronic intermittent peritoneal dialysis (ipd) were studied. total protein excretion in the peritoneal fluid was 21.5 +/- 2.1 g/24 h and did not vary with the mode ... | 1989 | 2916576 |
| intestinal maturation: characterization of mitochondrial calcium transport in the rat. | the mitochondria play a major role in the regulation of intracellular calcium. despite the fact that the enterocytes receive the majority of absorbed calcium, the role of the intestinal mitochondria in calcium transport during maturation is not known. therefore, the current studies were designed to characterize calcium pump activity of jejunal mitochondria of rats during maturation (suckling, weanling, and adolescent rats). the functional integrity of the intestinal mitochondria of suckling and ... | 1989 | 2919110 |
| an evaluation in pigs of nobi-vac ar and an experimental atrophic rhinitis vaccine containing p multocida dnt-toxoid and b bronchiseptica. | the trial involved eight large white sows obtained from a closed experimental specific pathogen free herd. four sows (two each for an experimental vaccine and for nobi-vac ar) were vaccinated twice (eight weeks and two weeks before parturition) with 2 ml of vaccine administered intramuscularly. two unvaccinated sows were used as an infected control group and two unvaccinated sows served as an uninfected control group. forty-six piglets (28 from vaccinated sows and 18 from unvaccinated sows) were ... | 1989 | 2919495 |
| on the suitability of the mtt-assay for the evaluation of mitogenic lymphocyte blastogenesis in swine. | using swine peripheral blood lymphocytes, we examined the suitability of a colorimetric tetrazolium (mtt) reduction test for the evaluation of mitogenic lymphoblastogenesis in comparison to radiolabelling methods. in our study, we did not find a correlation between the 3h-thymidine- or 14c-thymidine uptake into dna and the mitochondrial mtt cleavage activity, comparable to that formerly demonstrated for mouse cytotoxic t cells and for sheep peripheral blood lymphocytes. with the mtt assay, mitog ... | 1989 | 2929196 |
| endocarditis due to pasteurella multocida with glomerulonephritis. | | 1989 | 2929640 |
| pasteurella multocida recovered from live turkeys: prevalence and virulence in turkeys. | swabs of the oropharynges of 801 live turkeys (621 meat birds and 180 breeders), collected from 15 flocks that had experienced an outbreak of fowl cholera and from 12 non-outbreak flocks, were screened for the presence of pasteurella multocida. turkeys from outbreak flocks were sampled within 2 to 9 weeks of the outbreak. forty-nine isolates of p. multocida were recovered from turkeys in 11 of the outbreak flocks, and none were recovered from turkeys in non-outbreak flocks. isolation rates varie ... | 1989 | 2930395 |
| fowl cholera immunity induced by various vaccines in broiler minibreeder chickens determined by enzyme-linked immunosorbent assay. | broiler minibreeder hens were vaccinated for protection against fowl cholera at 12 and 21 weeks of age using several vaccination schemes, which included a live pasteurella multocida (cu strain) vaccine, two commercial polyvalent fowl cholera oil-based bacterins, and two experimentally prepared polyvalent oil-based bacterins. some treatment groups received only live or killed vaccines, whereas others received a live vaccine at 12 weeks followed by a killed product at 21 weeks. at 42 weeks of age, ... | 1989 | 2930408 |
| platelet deposition on vascular grafts. the accuracy of in vivo quantitation and the significance of in vivo platelet reactivity. | an in vivo platelet imaging system utilizing indium-111-labeled platelets and technetium-99m-labeled red cells was used to serially study and compare platelet deposition on autologous external jugular vein grafts, autologous arterial grafts, polytetrafluoroethylene (gore-tex) and two dacron (meadox and usci) small diameter (4mm) vascular grafts implanted end-to-end in canine carotid and femoral arteries. this method of quantitating platelet deposition was validated by correlating deposition meas ... | 1986 | 2937375 |
| complement, membrane glycoproteins, and complement receptors: their role in regulation of the immune response. | to determine the effect of complement on the normal antibody response we studied seven patients with genetically determined complement component deficiencies, guinea pigs deficient of c4 and c2, respectively, and two patients whose neutrophils and monocytes lack the c3bi receptor. patients deficient of early complement components (c4, c2, c3) have abnormal antibody responses to the t-cell-dependent antigen, bacteriophage phi x 174. complement-deficient guinea pigs (c4, c2) produce less antibody ... | 1986 | 2941194 |
| trefoil balloon for percutaneous valvuloplasty. | a new balloon (trefoil balloon) was developed to avoid complete interruption of blood flow during percutaneous balloon valvuloplasty. it consists of three identical 2- to 4-cm-long angioplasty balloons mounted in a parallel fashion on a single catheter. filled simultaneously, they assume the cross section of a rosette, allowing for continued blood flow. trefoil balloons of various sizes were tested in five dogs (aorta and aortic valve). they created a gradient ranging from 5 to 40 mmhg where con ... | 1986 | 2944597 |
| changes in the nasal mucosa of specific-pathogen-free neonatal pigs infected with pasteurella multocida or bordetella bronchiseptica. | | 1986 | 2945021 |
| malignant clonal expansion of large granular lymphocytes with a leu-11+, leu-7- surface phenotype: in vitro responsiveness of malignant cells to recombinant human interleukin 2. | a 14-year-old japanese female with neutropenia showed malignant proliferation of the large granular lymphocytes (lgls). these lgls were e rosette+ and fc(igg) receptor+ and therefore are referred to as t gamma lymphocytes. they were also leu-11+ and okt11+; however, they were clearly negative for leu-7, okt3, okt8, okm1, and hnk-1 antigens as well as for terminal deoxynucleotidyl transferase activity. karyotype analysis revealed 47, xxx. the lgls showed no rearrangement of t cell receptor c beta ... | 1986 | 2945603 |
| siderophore production by pasteurella multocida. | pasteurella multocida grown under conditions of iron deprivation secreted into the culture medium a growth-enhancing factor which functioned as a siderophore. the siderophore was found to be neither a phenolate nor a hydroxamate by chemical tests and bioassays and was given the trivial name multocidin. multocidin was partially purified and found to be a highly polar, nonaromatic, and dialyzable compound. this is the first report demonstrating the production of a siderophore by p. multocida. | 1986 | 2946642 |