| specific antibody response to pasteurella multocida. | six patients with culturally proven pasteurella multocida infection were evaluated serologically. the infections were 1 foot abscess, 1 septicemia, 3 bronchitis and 1 bronchopneumonia. most of them were elderly women closely exposed to pets or domestic animals. the serotypes of the strains were determined in 5 cases (3 a3, 2 a7). specific antibodies against capsular and somatic antigens of p. multocida were determined by indirect hemagglutination and agglutination respectively. the antibodies we ... | 1987 | 2445019 |
| galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons. | galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ... | 1987 | 2446770 |
| distribution of a monoclonal antibody-recognized protective protein immunogen on the outer membranes of pasteurella multocida rabbit isolates. | the distribution of a monoclonal antibody (mab)-recognized protective protein immunogen on the outer membrane of 153 pasteurella multocida rabbit isolates was determined by dot blot (db) analysis. mab 1608 reacted with 36 (24%) of the 153 clinical isolates. the db-positive clinical isolates expressed capsular antigens a, d, and nontypable and somatic antigens 2, 3, 10, 12, 15, and nontypable. western blot (immunoblot) analysis with adsorbed and eluted mab 1608 confirmed that the antigenic determ ... | 1988 | 2457598 |
| [a case of isolation of pasteurella multocida from blood clot from a febrile patient]. | | 1989 | 2481088 |
| [verification of antibiotic treatment after animal bites among patients at the antirabies center in rome]. | the study aimed at searching whether antibiotic prophylaxis was practised after animal bites, and, in this case, what were the criteria adopted. it was performed by considering 162 outpatients requiring assistance after animal bites at the antirabies centre of rome, italy. in this circumstances, prevention of bacterial and viral infections is performed by wound detersion, by administering anti-tetanus and anti-rabid prophylaxis where required by italian policy, according to the patient's history ... | 1989 | 2483634 |
| [pasteurella multocida bacteremia not related to animal bite or scratch]. | | 1989 | 2490732 |
| serotyping of isolates of pasteurella multocida from chickens. | | 1989 | 2499301 |
| [studies on growth of pasteurella multocida on btb agar]. | it has been reported that the pasteurella multocida does not grow on the btb agar. therefore, this medium has been used as selective and differential medium for pasteurella multocida. however, we have experienced that some of the pasteurella multocida from the patient's materials grew on the btb agar. here, we will report on the studies of the growth of the pasteurella multocida strain on the btb agar. ten strains of pasteurella multocida from humans and animals were used as the test strains. th ... | 1989 | 2501429 |
| prevention of haemorrhagic septicaemia in buffaloes and cattle with a live vaccine. | young cattle and buffaloes were vaccinated subcutaneously and intradermally with a live vaccine containing pasteurella multocida serotype b:3,4. twelve months after vaccination three of five young cattle in the subcutaneously vaccinated group and three of four in the intradermally vaccinated group were protected against serotype b:2 challenge. eleven buffaloes vaccinated subcutaneously and two vaccinated intradermally survived the same challenge 13 months after vaccination. | 1989 | 2502878 |
| evaluation of bovine antibody responses to haemorrhagic septicaemia vaccine. | elisa and immunoblotting techniques were used to examine the humoral immune response to pasteurella multocida, in bovine sera from indonesia and malaysia. elevated levels of antibody to a crude lipopolysaccharide preparation were found in vaccinated animals. in addition to the response to lipopolysaccharide, antibodies from the vaccinated cattle strongly labelled five to six of the 40 protein bands in this organism. | 1989 | 2508206 |
| normal and abnormal metabolism in injured rat brains by p-31 nmr spectroscopy. | | 1989 | 2519845 |
| rabbit pasteurellosis: respiratory and renal pathology of control and immunized rabbits after challenge with pasteurella multocida. | gross and microscopic lesions of pasteurellosis were studied in control and immunized pasteurella-free rabbits after challenge with virulent pasteurella multocida. pathologic responses were compared in rabbits immunized intravenously or mucosally with p. multocida or with j5, a cross protective core lps mutant of e. coli. all rabbits were challenged conjunctivally with approximately 2xld50 of p. multocida. rabbits were necropsied and examined for histopathology of the respiratory tract and kidne ... | 1989 | 2520448 |
| evaluation of the specificity of pasteurella multocida somatic antigen-typing antisera prepared in chickens, using ribosome-lipopolysaccharide complexes as inocula. | purified lipopolysaccharides (lps) from 16 serotypes of pasteurella multocida were complexed with aspergillus fumigatus ribosomes. the complexes were used as inocula to prepare antisera, in chickens, for somatic antigen typing by the gel diffusion precipitin test (gdpt). antisera made against 15 of 16 lps reacted with their respective specific heat-stable antigens in the gdpt and homologous lps in the passive hemagglutination test. antisera could not be made against serotype 15 lps. correlation ... | 1989 | 2521991 |
| toxicity of haemophilus pleuropneumoniae to porcine lung macrophages. | viable haemophilus pleuropneumoniae bacteria were toxic for porcine alveolar macrophages in vitro. this cytotoxic effect proved to be dose-related. a cell-free extract of h. pleuropneumoniae, heat-killed bacteria, and a pasteurella multocida field strain were nontoxic. when macrophages were cultured with h. pleuropneumoniae bacteria in a ratio of 100 macrophages to six bacteria, ultrastructural signs of cellular degeneration were observed within 1 h. this degeneration was observed in macrophages ... | 1989 | 2526407 |
| protection against progressive atrophic rhinitis by vaccination with pasteurella multocida toxin purified by monoclonal antibodies. | pasteurella multocida toxin was purified by affinity chromatography and inactivated by treatment with formaldehyde before use as a single component vaccine against progressive atrophic rhinitis in pigs. twenty pregnant gilts which were vaccinated twice before farrowing with either low or high doses of the purified toxoid, developed dose-dependent positive serum and colostrum titres to the toxin and, unlike the progeny of 10 untreated control gilts, the offspring of the vaccinated gilts also had ... | 1989 | 2528856 |
| [pasteurella multocida pericarditis]. | | 1989 | 2530524 |
| [human pulmonary infection by a strain of pasteurella multocida producing beta-lactamase]. | | 1989 | 2531415 |
| protection by toxoid-induced antibody of gnotobiotic piglets challenged with the dermonecrotic toxin of pasteurella multocida. | a crude dermonecrotic toxin (dnt) of pasteurella multocida (p.m.) type d was prepared by repeated sonication and freezing. it was sterilized by filtration. a toxoid was then made and pigs were hyperimmunized with it to get an antiserum. a control serum was obtained by hyperimmunization of pigs with a preparation derived from nontoxigenic p.m. type d in the same manner as the toxoid. three gnotobiotic piglets were injected with the antiserum. this resulted in neutralization indices (ni) of 25 in ... | 1989 | 2532834 |
| [septic arthritis caused by pasteurella multocida. treatment with a fluoroquinolone]. | | 1989 | 2534180 |
| in vitro studies with dimethyldithiocarbamate, possible new antimicrobial for use in poultry. | tests were conducted to determine the in vitro efficacy of the dithiocarbamate analogue, dimethyldithiocarbamate (dmdtc), against selected poultry pathogens. organisms studied were two bacteria, pasteurella multocida and escherichia coli, and a mold, aspergillus fumigatus. zone of inhibition and the minimum inhibitory concentration were determined for each organism. dmdtc was effective in vitro against all organisms tested, with a. fumigatus showing greatest overall sensitivity. | 1989 | 2539077 |
| multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription. | transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ... | 1989 | 2542891 |
| appearance of a late stage during mammalian dna replication when cells resume formation of 10 kb dna replication intermediates. | after the joining of human large dna replication intermediates and before the appearance of mature chromatin dna, there exists a distinct stage--'the post-elongation stage'. this stage reappears during recovery of dna synthesis simultaneously with the reappearance of a large dna replication intermediate, 10 kb dna. | 1989 | 2546825 |
| in vivo studies with dimethyldithiocarbamate, a possible new antimicrobial for use against aspergillus fumigatus in poultry. | dimethyldithiocarbamate (dmdtc), the carbamate analogue, was tested for therapeutic efficacy in a series of in vivo challenge trials using 5- and 10-week-old white leghorn chickens. challenge organisms were pasteurella multocida x-73, escherichia coli o1:k1, and aspergillus fumigatus. birds were evaluated for survival rates, lesion scores, and the rate at which the bacteria or mold could be reisolated following challenge. results showed dmdtc to be ineffective against p. multocida and e. coli at ... | 1989 | 2549938 |
| [pathologic consequences of a severe influenza outbreak (swine virus a/h1n1) under natural conditions in the non-immune sow at the beginning of pregnancy]. | pathological consequences of a severe outbreak of swine influenza (h1n1 virus) in the non immune sow at the beginning of pregnancy, under natural conditions. a sudden acute outbreak of fever, depression, anorexia and coughing in a group of nulliparous sows from a herd that was currently under epidemiological investigation lead to build a particular disposal of observation. the clinical signs were daily recorded including rectal temperature. blood was taken from the sows at the beginning of the t ... | 1989 | 2550169 |
| characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli. | the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ... | 1989 | 2556396 |
| efficacy of a live pasteurella multocida vaccine for the prevention of experimentally induced bovine pneumonic pasteurellosis. | seventeen holstein-friesian calves weighing an average of 139.8 +/- 13.5 (mean +/- standard deviation) kg were used in a study to determine the efficacy of a live vaccine containing of pasteurella multocida a:3 and pasteurella haemolytica a:1. eleven calves received the vaccine by intramuscular injection in the right shoulder, whereas six calves received vaccine diluent and served as non-vaccinated controls. fourteen days following vaccination (day 15) all calves were inoculated deep intranasall ... | 1989 | 2558441 |
| practical application of nucleic acid techniques to avian disease problems. | a workshop in which 17 practicing scientists participated was intended to address primarily people who use or could use biotechnology in their work and was confined to five techniques. endonuclease fingerprinting and mapping involved cleaving nucleic acid with a specific restriction enzyme and separating the nucleic acid fragments by electrophoresis. field and vaccine isolates of pasteurella multocida could be distinguished; salmonella enteritidis could be divided into three groups; chlamydia co ... | 1989 | 2559697 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| [topoisomerase i from human placenta. functional activity of products of expression of cloned cdna fragments]. | immunoscreening of the human placenta cdna-library in the expression vector lambda gt11 using non-isotope detection based on the avidin-biotin system allowed to identify a number of clones encoding human topoisomerase i. the fusion protein from an extract of escherichia coli cells infected with the recombinant phage lambda gt11 interacts with the monoclonal antibody raised against topoisomerase i from calf thymus; the dissociation constant being 5.7.10(-8) m. the restricted dna fragments coding ... | 1989 | 2561176 |
| transfer and properties of some natural and suicide replicons in pasteurella multocida. | we tested the transfer of several plasmids and transposons from escherichia coli to pasteurella multocida by filter mating. two plasmids, prktv5 (prk2013::tn7) and puw964 (prktv5::tn5), were derived from prk2013--a narrow-host-range plasmid with the broad-host-range incp conjugation genes. most p. multocida transconjugants obtained with prktv5 had tn7 insertions in the chromosome but some had insertions of the whole plasmid. by contrast, all the transconjugants obtained with puw964 had insertion ... | 1989 | 2561489 |
| wheat germ agglutinin but not concanavalin a modulates protein kinase c-mediated phosphorylation of red cell skeletal proteins. | human red blood cells contain protein kinase c (pkc) which acts exclusively on the membrane skeletal proteins band 4.1, band 4.9 and adducin. pkc activity can be stimulated by the addition of the phorbol ester 12-o-tetradecanoyl phorbol 13-acetate to intact cells. phosphorylation of band 4.1 by pkc in vitro results in a dramatic reduction in band 4.1 binding to spectrin and actin, as well as to the cytoplasmic domain of band 3. here we show that the lectin wheat germ agglutinin (wga), which bind ... | 1989 | 2583288 |
| long-term influence of enteric infection on jejunal propulsion in guinea pigs. | the intrinsic fluid-propelling behavior of guinea pig jejunal segments was reportedly altered by primary infection with trichinella spiralis from day 10 to day 20 postinoculation. after that time propulsive behavior returned to normal. the objective in the investigation reported here was to determine if jejunal responsiveness to an infection is influenced by prior exposure to the parasite. accordingly, jejunal propulsion was examined in guinea pigs reinfected 2 mo after the initial inoculation w ... | 1989 | 2583412 |
| yersinia enterocolitica 08 "american strain" isolated in italy. | a strain of yersinia enterocolitica serotype 08: b,e,f,i, "american strain", was isolated from a child suffering from febrile enteritis, without any history of foreign travel. such a strain, very uncommon in italy, though isolated from a sick child, lacked both in vivo and in vitro virulence-associated characteristics as lethality in mice, spontaneous autoagglutination and calcium dependency, traits correlated to a lack of the virulence plasmid. | 1989 | 2586336 |
| [bronchial infection and brain abscess caused by pasteurella multocida]. | the authors report a case of pasteurellosis with abscess of the brain consecutive to a bronchopulmonary infection in a woman with old-standing bilateral bronchiectasis. the prevalence of respiratory infections caused by pasteurella multocida is low, but it is certainly underestimated. bronchial and/or pleuro-pulmonary infections occur in subjects with reduced local and/or systemic defences. the respiratory system is colonized by direct or indirect contagion, usually in contact with pet animals c ... | 1989 | 2587896 |
| pasteurella multocida occurs in a high frequency in the saliva of pet dogs. | pasteurella multocida is a frequent cause of infection after animal bites. in contrast to earlier reports, p. multocida appeared to be as common among dogs as among cats. we found 17 (81%) of 21 pet dogs to harbour p. multocida in their saliva. at normal contact, the risk of transmission from dogs to humans seems to be negligible. only 1/27 dogs owners was found to harbour the organism. none of 13 cat owners or 23 persons without animal contacts harboured p. multocida. | 1989 | 2587958 |
| [the effect of biotechnological treatment of milk-replacer feeding mixtures on hematologic and biochemical parameters and its utilization in calves]. | a trial was performed with cellulase administration (3 cx per g of ingested feed dry matter) to early weaned calves fed minimum amounts of milk replacer (21 kg per milk nutrition period) and sweet (c, d) and sour (a, b) diets. the groups b and d were supplemented with an enzyme till the age of 90 days. the calves were given free choice of cot concentrate mixture, hay and water. liveweight gains, feed and nutrient intake and health condition of calves were the characteristics investigated in this ... | 1989 | 2588429 |
| [activity of the ovarian follicle system in cows with various puerperal courses]. | the presence of the various categories of follicles (less than 0.05; 0.5-1.0; 1.0-1.5; less than 1.5 cm) in the ovaries was examined by palpation, endoscopically and post mortem in cows with a physiological puerperium (n = 5), with puerperal endometritis (n = 5), and with retention of afterbirth (rs, n = 5) from the second to the 20th day post partum (p.p.). the largest proportion (82.6%) of the smallest follicles (less than 0.5 cm) was recorded between the fourth and seventh day p.p. in clinica ... | 1989 | 2588431 |
| [the occurrence of marek's disease in genetically different groups of chickens]. | the occurrence of marek's disease was studied under laboratory conditions in four genetically different groups of chickens (brown leghorn, f1 hybrids of the cb x ia inbred lines, pullets of the paternal branch of the grandparent stock of the hybro meat type, and final hybrids of the white hisex layer type) after infection with the georgia strain of marek's disease (md) virus, used in two doses (1600 and 16,000 pfu per one bird). md was diagnosed on the basis of the occurrence of macroscopic tumo ... | 1989 | 2588434 |
| [corneal abscess caused by pasteurella following cat scratch injury]. | authors report a pasteurella multocida corneal abscess after a scratch from a cat. the evolution was torpid and needed a transfixiant keratoplasty. they recall bacterial tanks, infestation ways, clinical manifestations and bacteriological identification. they note the low occurrence of ophthalmological manifestations and the sensibility of pasteurella to various antibiotics, especially beta lactams, phenicol and cyclins. | 1989 | 2590970 |
| comparison of methods for the sampling and isolation of toxigenic pasteurella multocida from the nasal cavity of pigs. | the efficacy of detecting toxigenic pasteurella multocida from nasal swabs of slaughtered and live pigs was assessed. the isolation of toxigenic p multocida from nasal cavities of slaughtered bacon pigs from two herds with atrophic rhinitis was reduced by immersion in the hot water tank by 25 per cent and 75 per cent. individual sows from one of the infected herds were repeatedly swabbed to find the best method of isolating toxigenic p multocida. toxigenic p multocida were isolated from 50 per c ... | 1989 | 2595096 |
| [specific properties of dna from fractions of isolated mouse synaptonemal complexes]. | | 1989 | 2598773 |
| [specific properties of dna from fractions of isolated mouse synaptonemal complexes]. | | 1989 | 2598773 |
| serum resistance is correlated with encapsulation of avian strains of pasteurella multocida. | encapsulated avian strains of pasteurella multocida possessing an a-type capsule were shown to be resistant to the bactericidal action of turkey serum, whereas unencapsulated variants as well as other unencapsulated strains were not. removal of the capsule from serum-resistant strain p1059-1 resulted in this strain becoming susceptible to the bactericidal effects of turkey serum. since complement was consumed when encapsulated or unencapsulated strain p1059-1 was incubated in turkey serum, we co ... | 1989 | 2609501 |
| primary structure of a ribonuclease from bullfrog (rana catesbeiana) liver. | a pyrimidine base-specific ribonuclease was purified from bullfrog (rana catesbeiana) liver by means of cm-cellulose column chromatography and affinity chromatography on heparin-sepharose cl-6b, which gave single band on sds-slab electrophoresis. the primary structure of the bullfrog liver rnase was determined. it consisted of 111 amino acid residues, including 8 half-cystine residues. from the sequence, it was concluded that three disulfide bridges in rnase a were conserved in the bullfrog rnas ... | 1989 | 2613682 |
| in vitro activities of cefcanel and some other cephalosporins against pasteurella multocida. | thirty-five strains of pasteurella multocida from humans and animals were tested for susceptibility to five cephalosporins by a broth dilution method. cefcanel showed high activity against all isolates (mic and mbc, less than or equal to 0.64 micrograms/ml). the corresponding figure for cefaclor and cefuroxime was 2.56 micrograms/ml. cefadroxil and cephalexin were the least active compounds tested. | 1989 | 2619280 |
| [isolation and characterization of ts-mutants of pasteurella multocida]. | temperature-sensitive mutants were isolated and characterised from wild strains of a, d, and a/d serotypes of pasteurella multocida by mutagenic treatment, using n-methyl-n-nitro-n-nitrosoguanidine. the mutants differed from parent strains by growth intensity at 41 degrees c and 43 degrees c as well as by reduced virulence to mice and decreased toxin formation capacity. mice were totally protected from intraperitoneal challenge infection, using the tenfold ld100, by two subcutaneous injections o ... | 1989 | 2619451 |
| fowl cholera in broilers. | pasteurella multocida, the etiologic agent of fowl cholera, was isolated from six broiler flocks in georgia during summer 1988. the flocks ranged in age from 20 to 46 days, represented four companies, and spanned a distance of 50 miles. increased mortality and lameness were the clinical signs present in all affected flocks. bacterial isolation and agar gel precipitation for somatic antigen serotyping revealed that three of the cases were caused by serotype 1,3, two by serotype 3,4, and one by se ... | 1989 | 2619670 |
| pasteurella multocida infection in japanese quail (coturnix coturnix japonica). | three flocks of japanese quail, approximately 75,000 birds each, experienced acute high mortality beginning at 24 to 28 days of age. gross lesions were absent or were composed of either multifocal small pale areas on livers and spleens or lungs slightly darker in color than normal. histopathology revealed multifocal splenic and hepatic necrosis and interstitial pneumonia. pasteurella multocida, serotype 3,4, was isolated from affected tissues. the quail were successfully treated with chlortetrac ... | 1989 | 2619671 |
| ontogeny of the estrogen receptor in the chick oviduct. | the distribution of estrogen receptor (er) in the chick oviduct was studied immunohistochemically with monoclonal antibody h222, known to recognize chick er [1]. the ontogeny of er appeared to be very dependent on cellular differentiation. in the undifferentiated oviduct er was located in the epithelial, mesothelial, stromal and smooth muscle cells. during differentiation er disappeared from the surface epithelium, mesothelium, stromal and smooth muscle cells. at the onset of differentiation the ... | 1989 | 2626020 |
| serum complement activity and serum enzymes in rats after a subcutaneous injection of toxin prepared from pasteurella multocida type d. | toxin produced by pasteurella multocida type d was investigated for its effect on serum complement and serum biochemistry in rats. rats were given a sublethal single subcutaneous injection of d toxin equivalent to 0.2 microgram/kg of body weight. serum obtained 1, 3, 5 and 7 days post-treatment was tested for complement activity, total bilirubin, aspartate aminotransferase (ast), alanine aminotransferase (alt) and alkaline phosphatase (alp). serum complement titers were significantly elevated (p ... | 1989 | 2629201 |
| [meningoencephalitis due to pasteurella multocida: clinico-laboratory study of a case in an infant]. | the authors report a case of pasteurella multocida meningoencephalitis in a 5 week-old female infant, with special attention to clinical, laboratory and evolutive features. a moderate neurological sequel was observed at follow-up examinations. a brief review of the importance of p. multocida in human pathology is presented on the basis of the international literature, since the authors did not find any brazilian reports. the most important feature on p. multocida is the prevalence of bacterial m ... | 1989 | 2634388 |
| effect of ok-432 on the lymphnode metastasis of mca-sarcoma cell lines: a new therapeutic approach. | the two unique highly metastatic mca-sarcoma cell lines have been established by the present authors. the inoculation of 1153ln, one of the cell lines, either into footpad or subcutaneously on the back of syngeneic mice resulted in the development of metastasis exclusively in almost all lymphnodes of the body. we evaluated the therapeutic effect of a streptococcal preparation, ok-432, on the lymphnode metastasis. two ke per mouse of ok-432 were injected intratumorally (it) at 4, 7 or 10 days aft ... | 1989 | 2637250 |
| study of the toxin-producing ability of pasteurella multocida in mice. | cell-free sonicated extracts and broth cultures of pasteurella multocida strains of pig origin were examined for their lienotoxicity in mice. p. multocida strains represented capsular types a and d with or without dermonecrotoxic (dnt) activity in the guinea pig skin test. mouse lienotoxicity test was suitable for determining the toxigenicity of p. multocida strains only when bacterium-free extracts were tested. in that case both toxigenic type a and d strains were lethal to intravenously inocul ... | 1989 | 2638810 |
| [adherence test in relation to caries activity]. | the amount of streptococcus mutans in saliva has been suggested as a way of estimating caries activity (klock; krasse, 1979; newbrum et al 1984; sabelli, 1985; thylstrop; fejerskov, 1988). this study was carried out no evaluate the relationship between an adherence test (formation of streptococcus mutans colonies which allows to group individuals according to level of risk) with the caries activity was determine in 137 children and samples of saliva (0.1 ml) were obtained from each of them. they ... | 1989 | 2639712 |
| portage transport of sulfanilamide and sulfanilic acid. | sulfanilic acid, in contrast to sulfanilamide, has poor in vitro antibacterial activity. paradoxically, it has been shown to be a more effective inhibitor than sulfanilamide of dihydropteroic acid synthase. in order to circumvent the presumed permeability barrier to sulfanilic acid, advantage was taken of the technique of portage transport. derivatives of the compound were prepared in which it was linked via its primary amino group to the alpha-carbon of glycine residues in di- and tripeptides. ... | 1989 | 2645404 |
| pharmacokinetics of single-dose administration of moxalactam in unweaned calves. | twenty-nine healthy 17- to 29-day-old unweaned israeli-friesian male calves were each given a single iv or im injection of 10 or 20 mg of moxalactam disodium/kg of body weight. serum concentrations were measured serially during a 12-hour period. serum concentration vs time profiles were analyzed by use of linear least-squares regression analysis and the statistical moment theory. the elimination half-lives after iv administration were 143.7 +/- 30.2 minutes and 155.5 +/- 10.5 minutes (harmonic m ... | 1989 | 2653122 |
| malignant hyperpyrexia. | | 1989 | 2653537 |
| effects of peptidases on non-adrenergic, non-cholinergic inhibitory responses of tracheal smooth muscle: a comparison with effects on vip- and phi-induced relaxation. | 1. the effects of peptidase enzymes on non-adrenergic, non-cholinergic (nanc) inhibitory responses of guinea-pig trachea to electrical field stimulation (efs), and on relaxations induced by vasoactive intestinal peptide (vip) and peptide histidine isoleucine (phi) have been examined. 2. alpha-chymotrypsin reduced both the magnitude and, particularly, the duration of the inhibitory response to efs, whereas papain reduced only the magnitude. aprotinin, a peptidase inhibitor prevented the effects o ... | 1989 | 2655804 |
| asymmetric dna bending induced by the yeast multifunctional factor tuf. | tuf is a yeast regulatory factor that binds to conserved dna sequence elements involved in gene activation or silencing as well as in telomere function. using gel electrophoresis analyses, we show here that tuf induces dna bending at a site located upstream of the recognition sequence (rpg box). several point mutations in the rpg box reduced tuf binding strength without affecting the extent of bending. selective proteolysis of tuf.dna complexes further suggested the existence of two separate pro ... | 1989 | 2656680 |
| pasteurella multocida infection from a cougar bite. a review of cougar attacks. | | 1989 | 2660410 |
| [the ophthalmogeriatric management of the aging human]. | | 1989 | 2660440 |
| inhibition of proteoglycan synthesis alters extracellular matrix deposition, proliferation, and cytoskeletal organization of rat aortic smooth muscle cells in culture. | arterial proteoglycans have been implicated in several important physiological processes ranging from lipid metabolism to regulation of smooth muscle cell growth. vascular smooth muscle (vsm) cells are the major producers of proteoglycans in the medial layer of blood vessels. to study functional consequences of alterations in vsm proteoglycan metabolism we used 4-methylumbelliferyl-beta-d-xyloside to inhibit proteoglycan synthesis in primary and early passage cultures of rat aortic smooth muscle ... | 1989 | 2661564 |
| what lies ahead in infant nutrition. | factors in human milk serve as stimuli for present and future investigation as supplements for infants. these substances involve macronutrients and micronutrients. some substances such as minerals may serve as guidelines for minima and maxima for infant nutrition. while nutritional studies are needed for the prevention of disease, simultaneous nutritional studies for low birth weight and ill infants will bring major alterations in understanding of nutritional needs and types of feedings to becom ... | 1989 | 2662411 |
| [effect of lipopolysaccharide toxin on lipid and protein composition of human serum low density lipoproteins]. | complexes of lipopolysaccharide (lps) b of salmonella typhimurium with human low density lipoproteins (ldl) formed during in vitro coincubation via spontaneous incorporation of lps (complex ldl-lps) or through the incorporation stimulated by the serum protein fraction (lps/ldl complex) were studied. the lps/ldl complex was shown to maximally bind 0.24 mg of lps per 1 mg of ldl protein, whereas the ldl-lps complex contained only 0.07 mg of lps per 1 mg of ldl protein. the observed incorporation o ... | 1989 | 2665826 |
| synthesis and antimicrobial evaluation of 20-deoxo-20-(3,5-dimethylpiperidin-1-yl)desmycosin (tilmicosin, el-870) and related cyclic amino derivatives. | a series of 20-deoxo-20-cyclic (alkylamino) derivatives of tylosin, desmycosin, macrocin and lactenocin was prepared by reductive amination of the c-20 aldehyde group. the majority of the compounds were prepared using metal hydrides (sodium cyanoborohydride or sodium borohydride) as the reducing agents and a suitable cyclic alkylamine. subsequently, a more convenient procedure was developed using formic acid as a reducing agent. the c-20 amino derivatives prepared from desmycosin exhibited good ... | 1989 | 2668243 |
| binding specificities of heat-labile enterotoxins isolated from porcine and human enterotoxigenic escherichia coli for different gangliosides. | the binding specificities of heat-labile enterotoxins (ltp and lth) isolated from porcine and human enterotoxigenic escherichia coli on human erythrocytes were studied by competitive binding assays using different gangliosides as inhibitors. the binding of 125i-labeled ltp to neuraminidase-treated human type a erythrocytes was most effectively inhibited by ganglioside gm1. ganglioside gm1 was 11 and 105 times more potent than gangliosides gd1b and gm2, respectively. gangliosides gd1a, gt1b, and ... | 1989 | 2670153 |
| modeling of palmitate transport in the heart. | transport of palmitate from the albumin-palmitate complex in the plasma to inside mitochondria where it undergoes beta-oxidation is a multistep process. albumin's large size prevents permeation via interendothelial clefts. palmitate dissociation from albumin in solution is too slow to provide an adequate supply of the unbound palmitate. the discovery that the dissociation occurs upon albumin binding to an endothelial surface receptor resolves the conundrum. palmitate transport across the luminal ... | 1989 | 2674667 |
| [a case of chronic bronchitis with pasteurella multocida possibly resulting from infection from a bird]. | a 60-year-old woman was admitted to our division for further evaluation of fever and purulent sputa. in sputum cultures performed when the patient had complained of an increase in symptoms on three occasions during the previous 6 months, pasteurella multicoida was usually detected. based on the fact that the bacteria had been detected from the patient's sputa after feeding a macaw, but was not detected after treatment of the bird with oflx, a diagnosis of respiratory tract infection by p. multoc ... | 1989 | 2677461 |
| pharmacokinetics of ceftazidime given alone and combination with probenecid to unweaned calves. | ceftazidime pharmacokinetic values were studied in unweaned calves given the antibiotic alone or in combination with probenecid. ceftazidime was administered iv to 9 calves at a dosage of 10 mg/kg of body weight and im (10 mg/kg) to 8 calves, to 7 calves (10 mg/kg plus probenecid [40 mg/kg]), and to 9 calves (10 mg/kg plus probenecid [80 mg/kg]). serum concentration-vs-time data were analyzed, using noncompartmental methods based on statistical moment theory. the data for iv ceftazidime administ ... | 1989 | 2679252 |
| cloning and expression of the pasteurella multocida toxin gene, toxa, in escherichia coli. | a chromosomal dna library of a toxigenic type d strain of pasteurella multocida subsp. multocida was established in escherichia coli. from this library two clones, spe308 and spe312, were identified by using a monoclonal antibody against the osteoclast-stimulating p. multocida toxin (pmt). extracts of these clones showed cytopathic activity identical to that of extracts of toxigenic p. multocida. the recombinant plasmids, pspe308 and pspe312, directed the synthesis of a protein with an apparent ... | 1989 | 2680987 |
| [action mode of insulin]. | | 1989 | 2681244 |
| [action mode of insulin]. | | 1989 | 2681244 |
| [comparison of myocardial thallium and beta-methyl iodophenyl pentadecanoic acid (bmipp) distribution in the cardiomyopathic hamster]. | the usefulness of fatty acid imaging in the detection of cardiomyopathy was evaluated by comparing thallium and bmipp myocardial distribution in bio 14.6 syrian hamster (25 week ages). autoradiography was performed in 3 using 3.7 mbq (100 microci) of 125i-bmipp and 37 mbq (1 mci) of 201tlcl. in vivo pin-hole imaging was performed in 3 using 37 mbq (1 mci) of 123i-bmipp and 37 mbq (1 mci) of 201tlcl. in all cardiomyopathic hamsters decreased uptake of bmipp compared to that of thallium was demons ... | 1989 | 2681896 |
| structure-activity studies of 20-deoxo-20-amino derivatives of tylosin-related macrolides. | reductive amination of the c-20 aldehyde group of tylosin and related macrolides yielded a large series of derivatives with potentially useful antibiotic properties. evaluation of these new compounds was conducted on the basis of: 1) broad antimicrobial spectrum in vitro, with particular emphasis on inhibition of pasteurella multocida and pasteurella haemolytica; 2) in vivo efficacy, especially when given orally, against p. multocida in experimental infections in chicks; and 3) bioavailability a ... | 1989 | 2684947 |
| management of human and animal bite wounds. | bite wounds, usually by dogs, cats, and human beings, affect one of two americans during his or her lifetime and 1 to 2 million americans annually. despite the relative frequency of bite wounds, there are few prospective studies to define optimal care; consequently, diverse methods are used. in this article i review the incidence, bacteriology, clinical spectrum, complications, and treatment of animal and human bite wounds. the spectrum of pathogenic bacteria that cause bite infections is broade ... | 1989 | 2685062 |
| mutagenesis by the anti-tumour drug nitracrine in escherichia coli. | the antitumour drug nitracrine [1-nitro-9-(dimethylaminopropylamino)acridine], known to be a potent frameshift mutagen in strains of salmonella typhimurium, also strongly reverts the lacz19124 frameshift marker in escherichia coli. the results in e. coli indicate that nitracrine causes dna damage which can be excised by the uvra,b,c excinuclease, can generate mutations by a reca-dependent mechanism, and gives enhanced yields of mutants when plasmid pkm101 is present. despite these observations, ... | 1989 | 2685595 |
| mutagenesis by the anti-tumour drug nitracrine in escherichia coli. | the antitumour drug nitracrine [1-nitro-9-(dimethylaminopropylamino)acridine], known to be a potent frameshift mutagen in strains of salmonella typhimurium, also strongly reverts the lacz19124 frameshift marker in escherichia coli. the results in e. coli indicate that nitracrine causes dna damage which can be excised by the uvra,b,c excinuclease, can generate mutations by a reca-dependent mechanism, and gives enhanced yields of mutants when plasmid pkm101 is present. despite these observations, ... | 1989 | 2685595 |
| [infectious factor diseases in domestic small animals (carnivorous and herbivorous fur animals, wool and meat rabbits]. | infectious factorial diseases of domesticated small animals are infection dependent diseases, whose pathogenesis is finally activated by additional, secondary factors, that influence the multiplying and spreading of latent and clinical symptomless infective agents present in the animals. these factorial diseases are not autonomous infectious processes, but only special types and courses of diseases by secondary activated infective agents. secondary factors may be of exogenous origin (housing, cl ... | 1989 | 2686619 |
| [atrophic rhinitis of swine--a multifactorial disease?]. | atrophic rhinitis is caused by toxigenic pasteurella multocida strains. the infection is supported by bordetella bronchiseptica and other widespread agents and by non-infectious factors damaging the nasal epithelium. the term "infectious multifactorial disease" means in german, that ubiquitous low-virulent agents cause disease when intensified by non-infectious factors. atrophic rhinitis does not belong to this group but is an infectious disease, caused by a specific agent and influenced by seve ... | 1989 | 2686620 |
| site of pyruvate formation and processing of mammalian s-adenosylmethionine decarboxylase proenzyme. | mammalian s-adenosylmethionine decarboxylase was expressed at a high level in an escherichia coli mutant deficient in this enzyme. the proenzyme form of this enzyme was cleaved and processed to the mature decarboxylase which contains two pairs of nonidentical subunits, the larger of which contains a pyruvate prosthetic group. in order to determine the site of formation of the pyruvate, two approaches were used. first, the mammalian s-adenosylmethionine decarboxylase produced in e. coli was purif ... | 1989 | 2687270 |
| immunoperoxidase evaluation of pneumonic lesions induced by pasteurella multocida in calves. | to evaluate the relationship between pneumonic lesions and distribution of bacteria, lungs from calves inoculated with pasteurella multocida were examined histologically by use of immunoperoxidase technique. pneumonic lesions fundamentally consisted of broncho-pneumonia with fibrinopurulent pleuritis. the lesions were confirmed to be associated with inoculated p multocida, using the immunoperoxidase technique. the p multocida antigen was detected not only in the bacterial clusters in the lesions ... | 1989 | 2692484 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| presence of the fish pathogen vibrio salmonicida in fish farm sediments. | the persistence of the fish pathogen vibrio salmonicida in fish farm sediments was studied by use of fluorescent-antibody techniques. the specificities of the monoclonal antibodies and polyclonal rabbit serum used in the study were tested against a number of vibrio strains, including 4 isolates from intestinal tracts of healthy fish and 98 isolates from sediments. v. salmonicida was detected in sediment samples from diseased farms several months after an outbreak of the disease. the bacterium wa ... | 1989 | 2696427 |
| computer-aided mapping of specific neuronal populations in the human brain. | antibody-staining methods and computer-aided microscopic systems have been used to generate high-resolution panoramic maps of specific neuronal populations in the human brain (4,6,11). this report focuses on the problems inherent in attempting high-resolution mapping of large brain sections, and describes how they are solved by computer-aided mapping. further applications of computers to the study of brain structure are considered. | 1989 | 2698651 |
| probenecid effect on cefuroxime pharmacokinetics in calves. | cefuroxime pharmacokinetics were studied in unweaned calves. the antibiotic was administered at 10 mg/kg to six calves i.v., to 12 calves i.m. and to ten of the previous 12 calves i.m. at 10 mg/kg together with probenecid at 40 mg/kg. intramuscular doses of cefuroxime alone at 20 mg/kg were given to seven calves; to five of these calves cefuroxime was also given together with probenecid at 40 mg/kg and at 80 mg/kg. the serum concentration-time data were analyzed using statistical moment theory ( ... | 1989 | 2704066 |
| in vitro evaluation of various quinolone antibacterial agents against veterinary mycoplasmas and porcine respiratory bacterial pathogens. | the in vitro activities of 12 quinolones and four antibiotics were determined against 15 veterinary mycoplasmal species and four species of bacteria commonly involved in respiratory infections in pigs. the newer quinolones were markedly more active in vitro against a wide range of mycoplasmas than nalidixic acid and the earlier quinolones. against mycoplasma hyopneumoniae ciprofloxacin was the most active quinolone with a geometric mean minimal inhibitory concentration (mic) against 16 strains o ... | 1989 | 2704885 |
| agarose soy casein digest medium for replacement of blood agar for potency determinations of live pasteurella vaccines. | blood agar, prepared with trypticase (bbl microbiology systems, cockeysville, md.) soy agar and 5% defibrinated bovine blood, is used for testing the potency of live pasteurella multocida and pasteurella haemolytica vaccines, but its potential for variation makes it undesirable to use in a standard assay method. tests done with rpmi 1640 and trypticase soy medium indicated that the benefits obtained by adding defibrinated blood to the trypticase soy agar medium were more likely due to neutraliza ... | 1989 | 2705764 |
| [experimental evaluation of the combined effect of the noise and aerosols in the air of steel mills]. | the analysis of 3 chronic experiments on white rats pertaining to the study of the combined effect of noise at 85 and 95 dba and aerosols containing manganese, chromium, nickel oxides and sio2 showed that there were noted the effects caused by their combined impact. the shifts were clearly marked in the state of the endocrine and cardiovascular systems. | 1989 | 2707606 |
| a protein toxin from pasteurella multocida type d causes acute and chronic hepatic toxicity in rats. | pasteurella toxin given subcutaneously to rats caused severe liver damage and growth suppression in doses as low as 15.6 ng. toxin was lethal at and above 31.25 ng. survival times were dose-dependent, and lesions differed with time of survival after toxin. rats dead of acute toxicity had focal hepatic necrosis. liver lesions were associated with diffuse endothelial damage, intravascular trapping of leukocytes, and degeneration of hepatocytes (characterized by glycogen depletion, development of v ... | 1989 | 2711572 |
| risk factors for oral cancer in women. | interviews were obtained from 125 women with oral cavity cancer and 107 female controls to assess the role of mouthwash use as a risk factor for oral cancer in women. in addition to detailed information on mouthwash use throughout adult life, information was obtained regarding smoking, alcohol consumption, general oral hygiene practices, and occurrence of nonmalignant conditions of the oral cavity. mouthwash use was not associated with increased oral cancer risk in terms of frequency, duration o ... | 1989 | 2713863 |
| effect of propranolol on accumulation of nefa in the ischemic perfused rat heart. | the effect of propranolol on the accumulation of non-esterified fatty acids (nefa) in the isolated, perfused working rat heart was investigated. ischemia was induced by lowering the afterload pressure to 0 mm hg for 20 min and for reperfusion, the pressure was raised to the pre-ischemic pressure (60 mm hg) for 20 min. the heart was frozen for biochemical studies immediately after ischemia or reperfusion. ischemia decreased mechanical function, increased the levels of palmitoleic, arachidonic and ... | 1989 | 2714364 |
| effect of propranolol on accumulation of nefa in the ischemic perfused rat heart. | the effect of propranolol on the accumulation of non-esterified fatty acids (nefa) in the isolated, perfused working rat heart was investigated. ischemia was induced by lowering the afterload pressure to 0 mm hg for 20 min and for reperfusion, the pressure was raised to the pre-ischemic pressure (60 mm hg) for 20 min. the heart was frozen for biochemical studies immediately after ischemia or reperfusion. ischemia decreased mechanical function, increased the levels of palmitoleic, arachidonic and ... | 1989 | 2714364 |
| genomic dna with transformation-related activity and melanoma antigen expression. | we have analyzed the mouse melanoma antigen with monoclonal antibodies established by syngeneic immunization. to further understand the structure of this antigen at a molecular level, we have cloned the genomic dna controlling the expression of melanoma antigen by cosmid library transfection and a monoclonal antibody. in the process of analyzing this dna fragment we found that it contained a gene related with transformation, which was proved by tumor formation in nude mice inoculated with nih/3t ... | 1989 | 2715661 |
| the effects of six environmental variables on pasteurella multocida populations in water. | the effects of protein, ph, temperature, sodium chloride (nacl), clays, sucrose, and their interactions on the survival and growth of pasteurella multocida were evaluated. pasteurellae populations declined rapidly in waters maintained at 2 c, compared to 18 c. increasing water soluble proteins by 175 micrograms/ml, and nacl by 0.5%, greatly enhanced survival of p. multocida, whereas variations in ph, clays, and sucrose had relatively minor effects. pasteurella multocida survived for over 1 yr in ... | 1989 | 2716103 |
| epizootiological features of avian cholera on the north coast of california. | an avian cholera (pasteurella multocida) epizootic was observed among wildfowl at the centerville gun club, humboldt county, california (usa) in january 1978. compared to their live populations and use of the area, coots (fulica americana) died in proportionately greater numbers than any other species. coots collected by gunshot were evaluated for sex and age composition, and morphometry from november 1977 through mid-january 1978 at this site. there was no substantial difference in the sex, age ... | 1989 | 2716104 |
| the effect of arterial oxygen content on the results of radiation therapy for epidermoid bronchogenic carcinoma. | anemia is believed to be an important prognostic factor in treating cancer patients by radiation therapy. one possible explanation for this is tumor oxygenation. with respect to tumor oxygenation, the arterial oxygen content (cao2) may be of more importance than the hemoglobin (hb) level. this study shows the relationship between the cao2 and tumor response to radiation therapy. forty-two patients with epidermoid bronchogenic carcinoma, treated by irradiation alone between april 1982 and march 1 ... | 1989 | 2722590 |
| field trial of a live streptomycin dependent pasteurella multocida serotype a:12 vaccine in rabbits. | a live, streptomycin dependent, pasteurella multocida (sdpm) serotype a:12 vaccine was evaluated for preventing pasteurellosis in two commercial rabbitries. rabbits were inoculated intranasally at 5 weeks old with either 0.25 ml of vaccine containing 10(8) colony forming units/ml or 0.25 ml of diluent (control). a proportion of rabbits received a second intranasal inoculation 1 month later. partial protection against p. multocida infection was observed 1 and 2 months after inoculation in rabbits ... | 1989 | 2724923 |
| fatal pasteurella multocida pneumonia in an iga-deficient cat fancier. | | 1989 | 2728443 |
| characterization of outer membrane protein-enriched extracts from pasteurella multocida isolated from turkeys. | outer membrane protein (omp)-enriched extracts of avian strains of pasteurella multocida were examined by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. culture medium did not have a significant effect on the omp profiles of strains of p multocida examined; however, in vivo propagation had an appreciable effect on the omp profile composition of the reference strain p-1059. such bacteria, expressed several additional omp in the 27-kd, 48-kd, 56-kd, 60-kd, 80-kd, and 94-kd molec ... | 1989 | 2729713 |
| isolation and interspecies-transfer of a plasmid from pasteurella multocida encoding for streptomycin resistance. | a plasmid encoding streptomycin-resistance could be detected in 13 of 32 pasteurella multocida-cultures isolated from cattle and swine. the plasmid of these cultures proved to be similar upon southern blot hybridization. it could be transformed into escherichia coli 490a, where it also expressed streptomycin resistance. | 1989 | 2733634 |