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in utero infection due to pasteurella multocida. 19921445989
nuclear-magnetic-resonance analysis of the capsular antigen of actinobacillus pleuropneumoniae serotype 9. its identity with the capsular antigen of escherichia coli k62 (k2ab), neisseria meningitidis serogroup h and pasteurella haemolytica serotype t15.the specific capsular antigen of actinobacillus pleuropneumoniae serotype 9 was characterized by one-dimensional and two-dimensional high-field nuclear-magnetic-resonance methods, and by chemical analyses, as a teichoic-acid-type polymer of a repeating unit having the structure [formula: see text] the basic polymer structure is identical to capsular antigens of neisseria meningitidis group h, escherichia coli k62 (k2ab) and pasteurella haemolytica serotype t15.19921446664
construction of a broad host range shuttle vector for gene cloning and expression in actinobacillus pleuropneumoniae and other pasteurellaceae.we have constructed a pair of broad host range expression vectors, pjff224-nx and pjff224-xn, based on plasmid rsf1010, which enable cloning and efficient expression of genes in actinobacillus pleuropneumoniae and pasteurella haemolytica and in escherichia coli. the vectors consist of the minimal autonomous replicon of the broad host range plasmid rsf1010 and a type ii chloramphenicol acetyl transferase gene for chloramphenicol resistance selection. in addition, they contain a gene expression ca ...19921448612
pasteurella multocida septicaemia in pigs. 19921449460
[osteomyelitis from a cat bite]. 19921450264
[wound infection caused by pasteurella dagmatis following a cat scratch]. 19921450272
effect of pasteurella multocida toxin on bone resorption in vitro.pasteurella multocida toxin (pmt), which is the primary etiologic factor in the pathogenesis of progressive atrophic rhinitis in pigs, was found to stimulate bone resorption in vitro. this stimulation was observed both in cultures of murine calvaria by measuring the release of calcium and of the lysosomal enzyme beta-glucuronidase and in murine long bone cultures by measuring the release of calcium. both systems showed the same dose response curve, with the maximal effect at a concentration of 5 ...19921452328
ovine pulmonary surfactant induces killing of pasteurella haemolytica, escherichia coli, and klebsiella pneumoniae by normal serum.pulmonary surfactant has been shown to play an increasingly important role in bacterial clearance at the alveolar surface in the lung. this study describes a bactericidal mechanism in which ovine pulmonary surfactant induces killing of pasteurella haemolytica by normal serum. to demonstrate killing, six bacterial species were incubated first with pulmonary surfactant for 60 min at 37 degrees c and then with serum for an additional 60 min at 37 degrees c. p. haemolytica type a1 strains 82-25 and ...19921452351
characterization and distribution of pasteurella species recovered from infected humans.during a 3-year period, all pasteurella strains recovered at the clinical microbiological laboratory, lund, sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. of 159 strains recovered from 146 infected humans, 95 were identified as pasteurella multocida subsp. multocida, 21 as pasteurella multocida subsp. septica, 28 as pasteurella canis, 10 as pasteurella stomatis, and 5 as pasteurella dagmatis. the homology within and between the ...19921452670
sequence analysis of the rob-1 beta-lactamase gene from actinobacillus pleuropneumoniae.the rob-1 beta-lactamase gene from actinobacillus pleuropneumoniae was cloned and sequenced. the structural gene encodes a 305 amino acid polypeptide. the rob-1 beta-lactamase gene sequence is identical to that derived from pasteurella haemolytica and only one amino acid different from that of haemophilus influenzae, suggesting that they are derived from the same ancestor, and transformed from one to another.19921455627
systemic and pulmonary antibody responses of calves to pasteurella haemolytica after intrapulmonary inoculation.systemic and pulmonary antibody responses of calves to pasteurella haemolytica were evaluated by measuring immunoglobulin production in blood for 9 days and in pulmonary lavage fluid for 7 days after intrapulmonary inoculation. clinical signs, pulmonary lesions, pulmonary and systemic inflammatory response, and amount of antigen in lavage fluid were used to evaluate the response of calves to challenge with p haemolytica. the pulmonary response consisted of production of igg, ige, and igm antibod ...19921456537
use of elisa to detect toxigenic pasteurella multocida in atrophic rhinitis in swine.the use of an enzyme-linked immunosorbent assay (elisa) as a means of detecting dermonecrotoxin-producing strains of pasteurella multocida was investigated. the assay was evaluated as a means to identify toxigenic p. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. the sensitivity and specificity of the elisa for detecting dermonecrotoxin-producing p. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. the elisa was highly sen ...19921457544
strain diversity within bovine pasteurella haemolytica serovar 2 respiratory tract isolates. 19921457554
interconversion of grp78/bip. a novel event in the action of pasteurella multocida toxin, bombesin, and platelet-derived growth factor.incubation of swiss 3t3 cells with [2-3h]adenine, as in other cell types, reveals the adp-ribosylation of grp78 (the 78-kda glucose-regulated protein, also known as bip, the immunoglobulin heavy chain-binding protein), a resident endoplasmic reticulum protein that assists in the processing of proteins destined for secretion or cell surface expression. here we show that pasteurella multocida toxin, a potent growth factor for cultured fibroblasts, decreased the adp-ribosylation of grp78/bip to 16 ...19921460024
pasteurella multocida endocarditis.human infection with pasteurella multocida is the leading cause of animal bite wound infection. life-threatening infection may occur in patients with a variety of underlying disorders and an immunocompromised state. infective endocarditis with p. multocida is very rare and only a few clinically diagnosed cases have been reported. described here is an autopsy case of a 61-year-old man with polycystic kidney disease who had p. multocida bacteremia and acute infective endocarditis with multiple bac ...19921462953
a comparison of the intratracheal, intravenous and intratonsillar routes of inoculation of goats with pasteurella haemolytica. 19921466144
the macrophage-activating properties of growth hormone.1. we compared the ability of growth hormone (gh) and a well-characterized macrophage-activating factor, interferon-gamma (ifn-gamma) to activate highly purified populations of alveolar macrophages. both gh and ifn-gamma primed macrophages triggered with opsonized zymosan to secrete superoxide anion (o2-) in vitro, but ifn-gamma was effective at a 40-fold lower concentration. antibody blocking studies demonstrated that the priming activity of gh was independent of ifn-gamma, and the activity of ...19921468117
[possibilities for the establishment of pneumonia-free swine herds by immunization and administration of cefquinome].the application of bordetella and pasteurella inactivated and adsorbed vaccines together with cefquinome to sows, piglets and weaners led to a significant reduction of the incidence of rhinitis atrophicans and pneumonia. the frequency of positive isolates of p. multocida, h. parasuis and a. pleuropneumoniae out of nasal swabs was reduced during the treatment.19921471972
serological evaluation of pasteurella multocida antigens associated with protection in buffalo calves.different antigens of pasteurella multocida carter's type 6:b including whole bacterium, antigen heated at 56 degrees c, antigen heated at 100 degrees c, sonicated antigen, capsular antigen, potassium thiocyanate extract, lipopolysaccharide and sodium salicylate extract were evaluated to assess protection in buffalo calves against haemorrhagic septicaemia. sera from calves with known protection status in experimental challenge were titrated by enzyme-linked immunosorbent assay (elisa) against al ...19921472736
avian cholera and waterfowl biology. 19921474673
molecular analysis of the aroa gene of pasteurella multocida and vaccine potential of a constructed aroa mutant.the aroa gene from pasteurella multocida was cloned by complementation of the escherichia coli aroa mutant ab2829 with a dna library constructed in puc18. the nucleotide sequence of the p. multocida aroa gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial aroa proteins. the cloned p. multocida aroa gene was inactivated by insertion of a kanamycin-resistance gene and reintroduce ...19921474900
some aspects of the epidemiology and control of salmonella typhimurium infection in outwintered suckler cows.two outbreaks of salmonella typhimurium infections affected outwintered, spring-calving suckler cows in late pregnancy. the infections spread rapidly both within and between groups of stock on the affected farms, with morbidity in the infected groups varying from 14.5 per cent to over 60 per cent, and mortality in adult cattle varying from 0 to 14.3 per cent. prophylactic measures included the use of antibiotics and killed vaccines against escherichia coli, salmonella dublin, s typhimurium, and ...19921475896
review of chemosystematics: multivariate approaches to oral bacteria and yeasts.there are several problems related to the classification and identification of bacterial and yeast species assigned to the genera actinobacillus, haemophilus, pasteurella, bacteroides, prevotella, porphyromonas, campylobacter, wolinella, treponema, candida, torulopsis, and saccharomyces, most of which belong to the resident oral microflora. the present review was written to demonstrate how multivariate analyses of data on cellular fatty acids, sugars, enzyme activities, and lysis kinetics during ...19921476049
application of ozone disinfection to remove enterococcus seriolicida, pasteurella piscicida, and vibrio anguillarum from seawater.survival of bacterial fish pathogens, including enterococcus seriolicida, vibrio anguillarum, and pasteurella piscicida, in ozonated seawater was determined in a batch system. bacterial counts of all fish pathogens decreased at more than 0.040 to 0.060 mg of total residual oxidants (tros) per liter, whereas no decrease in viable counts was observed at less than 0.018 to 0.028 mg of tros per liter. the 99% inactivation point was achieved at concentrations of 0.111 mg/liter for e. seriolicida, 0.0 ...19921476447
interaction between bordetella bronchiseptica and toxigenic pasteurella multocida on the nasal mucosa of spf piglets.the interaction between bordetella bronchiseptica and type d toxigenic pasteurella multocida was studied in five groups of 4 specific-pathogen-free (spf) piglets each. at 28 days of age, piglets of groups 3 and 4 were inoculated into both nostrils with 10(8) colony-forming-units (cfu) of a non-dermonecrotic toxin (dnt)-producing, phase i strain of b. bronchiseptica. piglets of groups 1 and 3 were treated intranasally with a sonic extract of the non-toxic strain of b. bronchiseptica and those of ...19921477161
the first outbreak of fowl cholera in muscovy ducks (cairina moschata) in japan.the first outbreak of fowl cholera occurred in a flock of muscovy ducks (cairina moschata) in okinawa prefecture of japan in november 1990. fifty (25%) of 200 birds in a farm died of an acute disease. remaining birds recovered after treatment with oxytetracycline. pasteurella multocida subsp. multocida was isolated in pure culture from all tissues tested from two dead birds. serovars of the isolates were identified as carter's capsular type a. heddleston's type 3.4.12, and namioka's type 5:a whi ...19921477178
pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. in this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. serum, tissue chamber fluid (tcf), and bronchial secretion (bs) concentrations of ceftiofur were measured by microbiological assay after the first and fourth ...19921477795
an analysis of the codon usage of pasteurella haemolytica a1.analysis of approximately 17 kbp of nucleotide sequences from three different regions of the genome of pasteurella haemolytica a1 showed that the mol% g+c of p. haemolytica a1 dna is 38.5%. when only the coding sequences (approx. 10 kbp) were analysed, a similar value of 38.8% was obtained. a comparison of the relative synonymous codon usage values of the cloned genes showed that p. haemolytica a1 has a very different codon usage pattern from that of escherichia coli.19921478451
[occurrence and drug resistance of bacteria pathogenic to the lungs from autopsy material of swine].eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. pasteurella multocida, haemophilus parasuis, bordetella bronchiseptica, actinobacillus pleuropneumoniae and pasteurella haemolytica were isolated in 59.1%, 29 ...19921481214
vaccination of turkeys with cell-free culture filtrate of pasteurella multocida: effects of dilution, iron chelation, and heterologous challenge.two experiments were done to further define cell-free culture filtrate (ccf) from pasteurella multocida and its endotoxin content in protecting turkeys against challenge. in the first experiment, the greater-than-30,000-molecular-weight fraction of p. multocida strain r44/6 (serotype 3/4/9/12) ccf was used in 10-fold dilutions given by air-sac inoculation or aerosol to vaccinate turkeys, which were subsequently challenged with either homologous (p-1059, serotype 3) or heterologous (x-73, serotyp ...19921485881
turkey macrophage and heterophil bactericidal activity against pasteurella multocida.bactericidal activity of turkey macrophages and heterophils was demonstrated in an in vitro colorimetric bactericidal assay. two vaccine strains and one field isolate of pasteurella multocida a:3,4 and a single isolate each of escherichia coli and staphylococcus aureus were compared for susceptibility to the bactericidal activity of turkey macrophages and heterophils. only p. multocida a:3,4-strain m-9 (the least virulent strain) was susceptible to macrophage bactericidal activity in the absence ...19921485882
pathogenic activities of live cells and extracellular products of the fish pathogen pasteurella piscicida.the pathobiological activities in vivo and in vitro of live cells and extracellular products (ecp) of eleven pasteurella piscicida strains of different origin were examined. infectivity trials showed that p. piscicida did not possess strict host specificity since the majority of the isolates were virulent for gilthead seabream, rainbow trout and turbot, with ld50 values ranging between 10(3) and 10(6) live cells. however, none of the strains tested were pathogenic for mice (ld50 > 10(8) cells)). ...19921487720
[septicemia caused by pasteurella pneumotropica]. 19921488425
characteristics of pasteurella multocida isolated from humans, swine and poultry in thailand.pasteurella multocida is a pathogen of animals and humans. most of the patients have been associated with animals but many cases had not contacted them. the failure to diagnose p. multocida infections is mostly due to misidentification on gram stained smears and inadequate laboratory identification techniques. in order to compile detailed characteristics of the organism we studied the physical and biochemical properties of 70 isolates of p. multocida - 17 human, 23 swine and 30 poultry. all isol ...19921488711
[oligoarthritis and bursitis caused by pasteurella multocida secondary to a cat scratch]. 19921489806
polymyxin b: pharmacokinetics of single doses given intravenously and intramuscularly to turkeys, and minimal inhibitory concentrations for escherichia coli and pasteurella multocida.the 50% and 90% minimal inhibitory concentrations (mic50 and mic90) of polymyxin b for avian escherichia coli and pasteurella multocida isolates were determined by the agar plate dilution method. polymyxin b at approximate mic level in serum was bactericidal for e. coli in 2 to 4 hours. aqueous polymyxin b sulfate was administered by a single bolus intravenous injection into turkeys at 10,000 iu/kg, and by a single bolus intramuscular injection at 5,000, 10,000 or 20,000 iu/kg. effective serum d ...19921492521
[antibiotic sensitivity of forty-four strains of group ef4 bacteria: study of minimum inhibitory concentrations using the agar dilution method].ef4 bacteria are found in animal saliva and may contaminate bite wounds. minimum inhibitory concentrations of 36 antimicrobials against 44 ef4 strains were determined using dilution in mueller-hinton agar. ef4 bacteria were susceptible to aminopenicillins, carboxypenicillins, ureidopenicillins, third-generation cephalosporins, fluoroquinolones, rifampicin, and trimethoprime-sulfamethoxazole. susceptibility was intermediate for penicillin g, low for macrolides and variable for aminoglycosides. ef ...19921495829
evaluation of an atrophic rhinitis vaccine under controlled conditions.a vaccine containing inactivated cultures of bordetella bronchiseptica, toxigenic pasteurella multocida type d and dermonecrotic p multocida type d toxoid in an oil-in-water adjuvant was given to seven sows, with seven others acting as controls. half the piglets in each litter were exposed intranasally when four days old to b bronchiseptica and when eight days old to toxigenic p multocida type d. there was considerably less sneezing in the litters of the vaccinated sows and when the piglets were ...19921496755
causes of mortality and parasites and incidental lesions in dolphins and whales from british waters.detailed post mortem examinations were carried out on 18 dolphins and whales found dead on the coast of the united kingdom. the commonest causes of death were pneumonia and entanglement in fishing gear. of the non-fatal conditions, parasitoses of various organs were common and there was a wide variety of other conditions. in total 124 diseases and other lesions were found, giving an average of 6.9 conditions per animal.19921496768
factors affecting endotoxin release from the cell surface of avian strains of pasteurella multocida.two avian strains of pasteurella multocida, a vaccine strain and a virulent field isolate, were investigated to determine their propensity to release endotoxin from the cell surface. both organisms released comparable amounts of endotoxin when plasma complement proteins were present, however the virulent strain did so without the loss of viability that occurred in the vaccine strain. blocking complement activity decreased the ability of plasma to elicit endotoxin release from the bacteria. when ...19921496810
passive protection of mice with antiserum to neuraminidase from pasteurella multocida serotype a:3.antiserum to a partially purified neuraminidase from pasteurella multocida, type a:3, was adsorbed with protease-digested p. multocida type 3 lipopolysaccharide (lps) to remove lps immunoreactivity. the lps-adsorbed antineuraminidase caused a 77% reduction in the neuraminidase activity of homologous p. multocida in an in vitro enzyme neutralization test. all 14 mice passively immunized with the adsorbed antineuraminidase were protected against challenge infection with homologous p. multocida in ...19921496818
polypeptides associated with pasteurella multocida infection in rabbits.polypeptides from whole cell preparations of pasteurella multocida serotypes a:12 and a:3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper. antigens were detected by immunoblot analysis, using sera from 3 groups of rabbits. sera were obtained from rabbits inoculated intranasally with p multocida serotype a:12 or a:3, from rabbits maintained in a rabbitry with enzootic p multocida a:12 infection, and from rabbits maintained in a r ...19921497178
[the importance of dermonecrotoxin and dermonecrotoxoid of pasteurella multocida for the calf].the i. m. application of dermonecrotoxin of p. multocida var. d led to an atrophy of nasal conchae, liver-swelling and to induration of liver and spleen. the intratracheal application caused a lobular pneumonia. the vaccination with toxoid resulted in an immunity against a challenge with toxin but not with p. multocida var. d. correlations between antitoxic serum-titers and immunity have to be investigated in future.19921497586
chronic frontal sinusitis in dairy cattle: 12 cases (1978-1989).chronic frontal sinusitis in 12 dairy cattle most often was associated with a history of dehorning, in which the sinus was entered (67%), or with respiratory tract disease (25%). the most common organisms isolated were actinomyces pyogenes and pasteurella multocida. signs of infection did not develop for months in some cattle and were often intermittent. the most common clinical signs included anorexia, lethargy, fever, frontal bone distortion, exophthalmos, abnormal posture, nasal discharge, an ...19921500335
[clinicobacteriological study of pasteurella multocida infection as a zoonosis. (2)--comparisons of p. multocida separated from patients and their pet dogs and cats].a total of 17 strains of pasteurella multocida, of which 13 were isolated from patients treated at nihon university itabashi hospital or nihon university surugadai hospital between april, 1984 and march, 1991 and 4 from 1 dog and 3 cats kept by the patients, were evaluated with respect to their biochemical properties, sensitivity to drugs, and serotype. the isolated strains were all considered to be pasteurella multocida subsp. multocida because of the agreement of their responses to indole, sor ...19921507482
serologic survey for selected arboviruses and other potential pathogens in wildlife from mexico.during 1988 and 1989, a serologic survey of wildlife was conducted in northeastern mexico to determine the presence, prevalence, and distribution of arboviruses and other selected disease agents. eighty mammal specimens were tested. antibodies to vesicular stomatitis-indiana, venezuelan equine encephalitis-mena ii, rio grande virus, and vesicular stomatitis-new jersey were detected predominantly in small mammals. deer and mouflon (ovis musimon) had antibodies to bluetongue and epizootic hemorrha ...19921512876
evidence of immunosuppression by bovine respiratory syncytial virus.respiratory syncytial virus (rsv) is a major respiratory pathogen in human infants and calves. calves and lambs infected with bovine rsv show mild clinical signs but they are more susceptible to secondary infection with pasteurella haemolytica. lambs infected with p. haemolytica 6 days after experimental infection with bovine rsv had significantly greater magnitudes of fever, higher disease and lesion scores and higher mortality rates than those infected with p. haemolytica or bovine rsv alone ( ...19921514055
a pathologic and bacteriologic study on otitis media in swine.a pathologic and bacteriologic study on otitis media in swine was performed on 237 swine, ranging in age from 1 day to 1 year. these 237 swine from eight selected farms were slaughtered due to unfavorable prognosis associated with clinical signs of illness. one hundred sixty-three (68.8%) of the 237 swine were found to be affected with otitis, though only a few swine showed clinical signs characteristic of the disease. one hundred fifty-one (63.7%), 53 (22.4%), and 39 (16.5%) had lesions in the ...19921514220
aerogenic pasteurellas and pasteurella-like organisms isolated in czechoslovakia. 19921520964
pasteurella haemolytica leukotoxin inhibits mitogen-induced bovine peripheral blood mononuclear cell proliferation in vitro.in this study we demonstrate that partially purified pasteurella haemolytica leukotoxin inhibits the proliferative response of bovine peripheral blood mononuclear cells (pbmc) to mitogens in vitro. inhibition of pbmc proliferation did not appear to be due to cell death. addition of a neutralizing anti-leukotoxin monoclonal antibody restored a normal proliferative response.19921522801
increased elastase activity in nasal mucus associated with nasal colonization by pasteurella haemolytica in infectious bovine rhinotracheitis virus-infected calves.four healthy calves were inoculated with pasteurella haemolytica serotype 1 by instillation of a broth culture into the middle nasal meatus of the left nostril. four weeks later, calves were exposed to infectious bovine rhinotracheitis virus by aerosol into both nostrils. all calves became ill, from approximately day 3 through day 10 after virus exposure, and shed increased amounts of nasal mucus. two calves were induced to shed p haemolytica by the virus infection, and 2 calves required reinocu ...19921524285
sequential development of antigens and toxins of pasteurella haemolytica serotype 1 grown in cell culture medium.pasteurella haemolytica was grown in nonsupplemented cell culture medium, or in medium supplemented with bovine serum albumin (bsa) for 24 hours. the production of leukotoxin (lkt) and endotoxin was sequentially evaluated, as were bacterial antigens associated with bacterial cell lysates and culture supernates. supplementation of medium with bsa had no effect on bacterial growth curves; however, lkt activity was detected earlier and was greater in culture supernates from bsa-supplemented media t ...19921524287
long-term study of aerobic bacteria of the genital tract in breeding bitches.the aerobic bacterial flora of the genital tract was characterized in 59 bitches in an 18-month study. the bitches represented 4 breeds and were from 3 kennels. collection of vaginal swab specimens for bacterial culturing was performed every month, except during estrus when specimens were collected every week (n = 826). the capsule of the swab containing transport media was broken before specimen collection to moisten the tip, which helped to reduce the number of negative cultures. all bitches h ...19921524290
long-term study of aerobic bacteria of the genital tract in stud dogs.the aerobic bacterial flora of the genital tract was characterized in 15 stud dogs in an 18-month study. the dogs represented 4 breeds and were from 3 kennels. bacterial samples from the prepuce and semen were collected every month, except in connection with matings, when they were collected weekly (464 samples). the dogs that were included all mated at least once during the study. the mean pregnancy rate, litter size, and pup mortality for the bitches with which they had mated were all within n ...19921524291
use of an indwelling bronchial catheter model of bovine pneumonic pasteurellosis for evaluation of therapeutic efficacy of various compounds.a model of bovine pneumonic pasteurellosis, using an indwelling bronchial catheter for inoculation and subsequent lavage of a single main stem bronchus of the lung, was evaluated in a preliminary efficacy trial of an experimental therapeutic compound. inoculation of 10(7) pasteurella haemolytica organisms into the bronchus consistently induced a focal pneumonic lesion with typical morphology of pneumonic pasteurellosis in the left or right caudal lung lobe. the experimental treatment caused sign ...19921524292
response of pasteurella haemolytica to erythromycin and dexamethasone in calves with established infection.a subcutaneous soft tissue infection model in calves was used to study the in vivo response of pasteurella haemolytica to erythromycin and dexamethasone. two tissue chambers were implanted sc in each of 12 calves. at 45 days after implantation, all tissue chambers were inoculated with an erythromycin-sensitive strain of p haemolytica. starting 24 hours after inoculation, calves were allotted to 4 groups of equal size and a 2 x 2-factorial arrangement of treatments was applied: 3 calves were give ...19921524293
distinct plasmid profiles of pasteurella haemolytica serotypes and the characterization and amplification in escherichia coli of ampicillin-resistance plasmids encoding rob-1 beta-lactamase.thirty-five isolates of pasteurella haemolytica from cattle or sheep were screened for the presence of plasmids and for resistance to a range of antibiotics. eight strains (four of serotype a1, three of serotype a2 and one untypable) contained plasmid dna and isolates of the same serotype had similar plasmid profiles, which were different from those of the other serotypes. all but one of the plasmid-bearing strains were isolated from pneumonic animals or from animals in contact with pneumonic ca ...19921527493
pasteurella multocida septicaemia in two calves. 19921530558
[pasteurella multocida infection following injury by an inert material]. 19921531266
fowl cholera epornitic: antigenic characterization and virulence of selected pasteurella multocida isolates.an epornitic of fowl cholera involving turkey flocks of several farms within a 15-mile radius in utah was studied. pasteurella multocida strains isolated from birds in affected flocks were antigenically characterized as a:1, a:3, and b:4, based on capsular sero-grouping and somatic serotyping results. experimental exposure of poults with each of two strains representing the rarely reported capsular group b indicated that both were virulent.19921533118
isolation of p multocida f-3, 4 from a stillborn snow leopard. 19921542981
septic arthritis due to pasteurella multocida.we have reported two cases of septic arthritis caused by pasteurella multocida, the first, septic polyarthritis unrelated to animal contact and the other, septic monarthritis after a cat scratch. serious infections with this gram-negative bacillus must be treated with intravenous penicillin, ampicillin, or a third-generation cephalosporin; aminoglycosides are not effective. a high index of suspicion is appropriate when there is a history of a recent animal-inflicted wound, though infection may a ...19921546361
phylogeny of 54 representative strains of species in the family pasteurellaceae as determined by comparison of 16s rrna sequences.virtually complete 16s rrna sequences were determined for 54 representative strains of species in the family pasteurellaceae. of these strains, 15 were pasteurella, 16 were actinobacillus, and 23 were haemophilus. a phylogenetic tree was constructed based on sequence similarity, using the neighbor-joining method. fifty-three of the strains fell within four large clusters. the first cluster included the type strains of haemophilus influenzae, h. aegyptius, h. aphrophilus, h. haemolyticus, h. para ...19921548238
pasteurella haemolytica a1 and bovine respiratory disease: pathogenesis.the severe fibrinonecrotic pneumonia associated with pneumonic pasteurellosis usually results from colonization of the lower respiratory tract by pasteurella haemolytica biotype a, serotype 1(a1). despite recent research efforts, the authors lack a detailed understanding of the interactions and host response to p. haemolytica in the respiratory tract. the authors hypothesize that management and environmental stress factors or viral infection alters the upper respiratory tract (urt) epithelium al ...19921548621
alveolar macrophage and neutrophil interactions in pasteurella haemolytica-induced endothelial cell injury.pasteurella haemolytica, the cause of fibrinous pleuropneumonia in cattle, produces extensive microvascular endothelial cell damage. this study investigated endothelial cell-leukocyte interactions by using a millicell coculture assay system that modeled the bovine pulmonary alveolar defense system and showed that p. haemolytica-mediated endothelial cell damage was enhanced by the presence of alveolar macrophages, presumably due to soluble alveolar macrophage products. the alveolar macrophage-enh ...19921552194
in utero infection due to pasteurella multocida in the first trimester of pregnancy: case report and review.to our knowledge, we report the first case of in utero infection in humans due to pasteurella multocida in the first trimester of pregnancy. after antibiotic therapy was initiated, the mother recovered rapidly from bacteremia but the fetus was spontaneously aborted. pathological examination of the fetus and placenta revealed acute deciduitis and chorionitis, indicating an infectious etiology for the abortion. the clinical and pathological features of this case are compared with those of the five ...19921554837
the effect of the intra-mammary inoculation of lactating ewes with pasteurella haemolytica isolates from different sources.lactating welsh mountain ewes were inoculated, 3 weeks after lambing, with between 1000 and 10,000 colony forming units of a number of isolates of pasteurella haemolytica. isolates from severe, acute mastitis in a ewe, from ovine and bovine pneumonic lesions and from the nasal cavity of healthy lambs, all gave rise to severe, acute mastitis that was clinically indistinguishable from that seen naturally. two isolates from the milk of ewes with subclinical mastitis did not cause clinical disease a ...19921556261
[detection of the nucleolar activity in alveolar macrophages of swine].with the help of the toluidine blue-staining-technique different characteristic shapes of alveolar macrophage nucleoli were detected, indicating differences in the rrna synthesis activity of these cells. after application of a pasteurella multocida aerosol the percentage of alveolar macrophages with a high rrna synthesis increased. no correlation between the nucleolar activity and the efficacy of the clearance of aerogenously administered pasteurella could be measured.19921559462
conditions for transformation of pasteurella multocida by electroporation.conditions for electroporation of plasmid dna into pasteurella multocida were determined for use in developing a cloning system to study virulence factors of p. multocida. the highest efficiency of transformation (1.25 x 10(7) cfu/micrograms dna) was obtained when 7.6 x 10(10) cells of p. multocida strain r473 were electroporated at 12.5 kv/cm (10 ms, 5 ng of pvm109). transformation efficiencies of cells prepared at mid-log-phase were approximately 0.5 log10 lower than early, late, or stationary ...19921560754
bite wounds and infection.one of every two americans will be bitten by an animal or by another person at some point. bites account for approximately 1% of all visits to emergency rooms; injuries inflicted by dogs are most common. the bacteria involved in infection of bite wounds include pasteurella multocida, staphylococcus aureus, staphylococcus intermedius, alpha-hemolytic streptococci, capnocytophaga canimorsus, and other members of the oral flora. anaerobic bacteria are present in approximately one-third of bite woun ...19921562653
bacteremia due to pasteurella multocida.pasteurella multocida should be considered as a possible etiologic agent in any infection that is the result of an animal bite or scratch. because of its opportunistic capability, it should be included among the possible pathogens in bacteremia, particularly in any patient with immunosuppression or liver cirrhosis, especially if there is a history of animal exposure.19921566154
comparative susceptibility of caponized and uncaponized tom turkeys to pasteurella multocida.when susceptibility to virulent pasteurella multocida was compared, there was no significant (p greater than 0.05) difference between caponized and uncaponized tom turkeys. neither was there any significant (p greater than 0.05) difference between the surviving caponized and uncaponized toms in the development of serum anti-p. multocida antibody. however, at 28 weeks of age, the average live body weight of the caponized toms was significantly (p less than 0.05) lower than that of the uncaponized ...19921567314
treatment of pasteurella multocida cellulitis with ciprofloxacin. 19921569315
survival of toxigenic pasteurella multocida in aerosols and aqueous liquids.the survival of toxigenic pasteurella multocida in air and liquids was studied to identify possible risk factors in the etiology of atrophic rhinitis. in aerosols, at low relative humidity (28%), the viability of toxigenic p. multocida 5 min after aerosolization was at least 22% of its initial value. viability at low relative humidity declined to 8% after 45 min. viability at high relative humidity (79%) was 69% after 5 min and declined to 2% after 45 min. survival of toxigenic p. multocida in l ...19921575496
[the significance of antibodies to pasteurella haemolytica a1 in the colostrum of cows and blood serum of calves].antibodies to p. haemolytica were detected in colostral sera of cows and blood sera of calves by means of indirect haemagglutination (iha) and elisa. the elisa titres of the colostral sera of the dams and of the blood sera of the calves showed a significantly positive correlation. there is a positive correlation between the titres of the two serological methods. the results of the challenge infection with p. haemolytica a 1 do not show a correlation between the titres and the severity of the dis ...19921575671
pasteurella multocida toxin. the characterisation of the toxin and its significance in the diagnosis and prevention of progressive atrophic rhinitis in pigs. 19921576005
cell surface characteristics and virulence in mice of pasteurella multocida.the virulence of three avian strains of pasteurella multocida was evaluated in mice. strains p-1059i (serotype a:3) and its uncapsulated variant p-1059b and strain 2723 (serotype a:16) were compared. capsular material thickness after polycationic ferritin labelling of dextrose starch agar (dsa)-grown p. multocida was shown to vary with the strain and was not always related to virulence. addition of alpha,alpha' bipyridyl (bip) (160 microm) to the culture medium did not affect capsule production ...19921576405
prevention of pasteurellosis. 19921576523
haemorrhagic septicaemia--a general review. 19921576525
further investigations on pasteurella multocida infections in feral birds injured by cats.a total of 64 pasteurella multocida strains (46 out of 11 different feral bird species, partly injured by cat bites, and 18 strains originating from clinically healthy cats) were biochemically differentiated. as a result, 67.4% of the strains from feral birds and 61.1% from the cats were classified as the subspecies multocida, whilst 21.7% and 27.8% were identified as the subspecies septica. the percentage of frequency for both the subspecies was of a comparable order of magnitude from the birds ...19921580104
pasteurella multocida toxin is a potent inducer of anchorage-independent cell growth.the growth of many normal cells requires contact with an adhesive substratum, a requirement that is frequently abrogated in the transformed phenotype. we have explored pathways that can lead to the anchorage-independent growth of cultured rat-1 fibroblasts. pasteurella multocida toxin (pmt), a 146-kda mitogenic protein, caused a striking increase in the formation of colonies (greater than 200 microns) from single cells in soft agar. the magnitude of the effect of pmt was greater than that achiev ...19921584759
comparison of antigens of pasteurella haemolytica serotype 1 grown in vitro and in vivo.to determine whether antigenic differences exist in pasteurella haemolytica serotype 1 grown in different culture conditions, the bacteria was grown on solid enriched medium, in broth culture, and in tissue chambers subcutaneously implanted in the flanks of calves. the organisms obtained by each culture method were comparable with respect to encapsulation and lipopolysaccharide content. in the bacteria grown in vivo, several unique high molecular-mass (greater than 150 kda) protein antigens were ...19921586014
colonization of the tonsils of calves with pasteurella haemolytica.tonsils of 10 calves were inoculated with pasteurella haemolytica (ph) and the degree of colonization was followed by collecting sequential tonsil. wash specimens. tonsils were colonized for at least 3 weeks after instillation of ph into the tonsillar sinus. calves with colonized tonsils responded with serum and nasal secretion antibody responses to ph and to leukotoxin. pasteurella haemolytica was detected in nasal mucus specimens of 2 calves during the week after inoculation of the tonsils, bu ...19921586016
molecular characterization of an rtx toxin determinant from actinobacillus suis.rtx cytolysins are a family of calcium-dependent, pore-forming, secreted toxins found in a variety of gram-negative bacteria. the prototypical member of this family is the alpha-hemolysin of escherichia coli. the rtx genetic determinants from seven members of the family pasteurellaceae, pasteurella haemolytica, actinobacillus actinomycetemcomitans, and a. pleuropneumoniae serotypes 1,5,7, and 9 were previously cloned and sequenced. using the leukotoxin determinant from p. haemolytica serotype a1 ...19921587585
cytological findings in bronchoalveolar lavage fluid from feedlot calves: associations with pulmonary microbial flora.samples obtained by bronchoalveolar lavage (bal) were used to evaluate pulmonary cytology in 59 feedlot calves with clinical signs of respiratory disease (cases) and 60 clinically normal comparison calves (controls). many calves in both case and control groups had inflammatory changes in the lower respiratory tract, as determined by changes in proportions in the bal differential cell count. approximately 35% of cases and 40% of controls showed a normal differential cell count. it therefore appea ...19921591654
pulmonary immunity in calves following stimulation of the gut-associated lymphatic tissue by bacterial exotoxin.antibodies in serum and pulmonary lavage fluids were measured in calves following stimulation of the gut-associated lymphatic tissue (galt) by inoculation of crude leukotoxin of pasteurella haemolytica into the duodenum through a surgically placed catheter. nine calves free of p. haemolytica were divided into two groups. group 1 received an intraduodenal (id) inoculation of leukotoxin and group 2 received an id inoculation of phosphate buffered saline. serum and pulmonary lavage fluids were coll ...19921591657
serological characterisation of pasteurella multocida strains isolated from wild ruminants as capsular serogroup b. 19921595164
hyaluronic acid and a (1----4)-beta-d-xylan, extracellular polysaccharides of pasteurella multocida (carter type a) strain 880. 19921596931
the effect of inoculation of pasteurella haemolytica into the lactating mammary gland of mice, rats, rabbits, sows and cows.an isolate of pasteurella haemolytica (a9), which consistently produced severe mastitis in ewes, was inoculated into the lactating mammary glands of a variety of species. mastitis did not develop after the inoculation of log-phase bacteria into the mammary gland of lactating mice, rats, rabbits or sows but did so in the mammary gland of two cows. another a9 isolate from a ewe with mastitis and an a1 isolate from a bovine pneumonic lung also induced mastitis in cows. thus, in this study, p. haemo ...19921602056
pasteurella infection in a total knee arthroplasty.hematogenous infection of a total joint arthroplasty is a serious complication that has well-known etiologies. one of the most unusual inciting events is a bite wound. pasteurella multocida is an anaerobic organism found in the mouths of mammals that has rarely been found to infect total knee arthroplasties. prompt recognition of such an infection and prophylactic treatment with a penicillinase-resistant penicillin should maximize the patient's opportunity to eradicate such an infection.19921603610
[purification, amino acid composition and n-terminal sequence of the major protein (protein h) of the outer membrane of pasteurella multocida].the major protein (protein h) of the outer membrane of pasteurella multocida was purified by size-exclusion chromatography after selective extraction with detergents. the protein forms homotrimers which are stable in the presence of sds at room temperature. upon treatment at 100 degrees c, the protein is fully dissociated by the detergent into monomers exhibiting an apparent molecular mass of 37 kda as estimated by electrophoresis. the amino acid composition of protein h is characterized by a lo ...19921606514
molecular cloning and expression of ptxa, the gene encoding the 120-kilodalton cytotoxin of actinobacillus pleuropneumoniae serotype 2.the genetic determinants of the 120-kda cytotoxin of actinobacillus pleuropneumoniae serotype 2 were isolated from a lambda dna library by a plaque immunoblot technique. expression of the 120-kda polypeptide was confirmed by western immunoblot analysis of infected escherichia coli cell lysates, which were shown to be toxic for porcine alveolar macrophages in vitro. the genetic determinants of the toxin were subcloned into the plasmid vector puc18. this plasmid (pptx1) directed the synthesis and ...19921612740
interaction of ruminant transferrins with transferrin receptors in bovine isolates of pasteurella haemolytica and haemophilus somnus.the interactions of ruminant transferrins with receptors on bovine isolates of pasteurella haemolytica and haemophilus somnus were compared by growth studies and direct and competitive binding assays. isolates of p. haemolytica were capable of utilizing and binding transferrin from sheep, goat, or cattle, whereas isolates of h. somnus were capable of utilizing and binding only bovine transferrin.19921612764
tumor necrosis factor alpha and interleukin 1 alpha enhance lipopolysaccharide-mediated bovine endothelial cell injury.alveolar macrophages (ams) are important in the host response to aerogenous pulmonary bacterial infections, such as pasteurella haemolytica-induced pneumonia in cattle. previous work has shown that ams enhance p. haemolytica-mediated pulmonary endothelial cell (ec) damage in vitro. the purpose of this study was to determine the mechanism of am-enhanced ec damage using an in vitro am-ec coculture system consisting of ams cultured on culture plate insert membranes and ecs in the underlying chamber ...19921613394
pasteurella multocida infection in total knee arthroplasty. case report and literature review.pasteurella multocida, a small gram-negative bacterium, is part of the normal mouth flora of many animals, including domestic cats and dogs. while commonly associated with infections in animals, it is a rare cause of human disease. the majority of pasteurella infections in humans occur with percutaneous inoculation of the organism following a bite by a cat or dog, although disease without antecedent animal exposure or with causal animal contact does occur. the spectrum of disease produced ranges ...19921613522
pasteurella multocida infection after a tasmanian devil bite. 19921623089
characterization of lipopolysaccharides from four pasteurella haemolytica serotype strains: evidence for presence of sialic acid in serotypes 1 and 5.highly purified lipopolysaccharides (lps) obtained from four strains of pasteurella haemolytica representative of four different serotypes were studied to ascertain their overall structural elements and sugar and fatty acid compositions. sds-page analysis revealed that each lps was of the smooth-type although they differed in migration patterns. somewhat unusual features of these lps included the presence of: (a) rhamnose in the core oligosaccharides of serotypes 2 and 3; and (b) sialic acid in ...19921624117
characterization of pasteurella multocida from nasal cavities of piglets from farms with or without atrophic rhinitis.a total of 137 strains of pasteurella multocida isolated from the nasal tracts of pigs with and without clinical atrophic rhinitis (ar) were studied for their biochemical, antigenic, and toxigenic characteristics. there were no major biochemical differences among the p. multocida isolates. capsular antigen types a and d were both present in the nasal cavities of the pigs with or without clinical ar. however, the prevalence of type d was higher on farms with pigs with ar. types a and d with diffe ...19921624554
characterisation and biological activity of monoclonal antibodies specific for pasteurella haemolytica a1 capsule and lipopolysaccharide.monoclonal antibodies (mab) against both pasteurella haemolytica a1 capsule and lipopolysaccharide (lps) were produced. anti-capsule mab reacted with the homologous a1 serotype only, whereas mab against lps reacted with p. haemolytica serotypes a2, a5, a8, a12, a14 and a16 but not with 33 bacterial species or rough lps mutant strains tested. both capsule and lps antigens were visualised on the surface of bacteria by immunogold electron microscopy. neither of the mabs demonstrated antibody-depend ...19921626366
vaccine efficacy for reducing turbinate atrophy and improving growth rate in piggeries with endemic atrophic rhinitis.two vaccines, based on formalin-killed whole cells of toxigenic pasteurella multocida type d and bordetella bronchiseptica combined with a partially toxoided cell extract of p multocida, were prepared with freund's incomplete adjuvant (vaccine 1) or by alum precipitation (vaccine 2). each was tested for safety and efficacy in reducing the severity of nasal turbinate atrophy and improving the growth rate of pigs in three western australian commercial piggeries with endemic atrophic rhinitis. in s ...19921627085
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