Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| aerobic and anaerobic bacterial isolates from horses with pneumonia or pleuropneumonia and antimicrobial susceptibility patterns of the aerobes. | frequency of aerobic and anaerobic isolates in 327 aspirates and in 123 pleural fluid samples from 327 horses with pneumonia or pleuropneumonia and antimicrobial susceptibility patterns of the aerobes were reported. of the 327 horses, 75% survived, 20% were euthanatized, and 5% died. tracheobronchial aspirates or pleural fluid specimens from 25 of the horses did not yield growth. of the remaining 302 horses, 221 had only aerobic organisms isolated, whereas only anaerobes were isolated from 6 of ... | 1991 | 2026535 |
| vaccination of turkeys with cell-free culture filtrate of pasteurella multocida. | turkeys given cell-free culture filtrate (ccf) of pasteurella multocida strain r44/6 orally, via air sacs, or subcutaneously mixed 1:1 with incomplete freund's adjuvant (ifa) at 6 and 9.5 weeks of age were compared with negative controls given bacteriologic medium and positive controls vaccinated with a commercial bacterin. at 13 weeks of age, serum antibody titers to p. multocida were detectable only in turkeys given ccf in ifa (low titers) and positive control turkeys (high titers), at which t ... | 1991 | 2029246 |
| effects of adjuvant-augmented germling vaccines in turkey poults challenged with aspergillus fumigatus. | turkey poults were vaccinated with combinations of two different germling preparations and three adjuvants (n-acetylmuranyl-l-alanyl-d-isoglutamine, pasteurella multocida lipopolysaccharide [lps], and avridine) at 1 and 2 weeks of age, and their immunity was challenged by sublethal exposure to aerosols of aspergillus fumigatus conidia at 1 month of age. fewer turkeys in the groups given vaccines prepared from germlings grown on dorset's and henley's medium (d&h) had organisms in lung tissue at 2 ... | 1991 | 2029265 |
| interaction of bovine alveolar macrophages with pasteurella haemolytica a1 in vitro: modulation by purified capsular polysaccharide. | preincubation of bovine alveolar macrophages with pasteurella haemolytica a1 purified capsular polysaccharide markedly reduced the phagocytosis of p. haemolytica a1 in vitro in a dose-dependent manner. both the percentage of macrophages with intracellular p. haemolytica and the mean number of bacteria per ingesting macrophage were decreased by treatment with capsular polysaccharide. untreated bovine alveolar macrophages had little ability to kill p. haemolytica a1 in vitro at bacteria to macroph ... | 1991 | 2031303 |
| oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections. | two hundred and seventy bacterial isolates were obtained from the pharyngeal tonsillar surface of 12 normal horses and 98 obligatory anaerobic bacteria were characterised. of these, 57 isolates belonging to 7 genera (peptostreptococcus (1); eubacterium (9); clostridium (6); veillonella (6); megasphera (1); bacteroides (28); fusobacterium (6)) were identified, and 16 of these were identified to species level (p. anaerobius (1); e. fossor (9); c. villosum (1); b. fragilis (1); b. tectum (2); b. he ... | 1991 | 2031304 |
| development of pneumonia in desert bighorn sheep after exposure to a flock of exotic wild and domestic sheep. | from 1986 to 1989, 5 desert bighorn sheep (3 ovis canadensis mexicana and 2 o c nelsoni), ranging in age from 2 to 3 years, were exposed to a flock of exotic wild and domestic sheep to potentially achieve naturally acquired pneumonia. pasteurella multocida was isolated from nasal samples from 4 of 6 sheep randomly sampled from the flock. bighorn sheep were exposed individually and each exposure period was a trial. treatment before and after exposure varied and included combinations of alpha inte ... | 1991 | 2032914 |
| the cytotoxin of actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kda polypeptide. | mutants of actinobacillus pleuropneumoniae strain hk 361 (serotype 2) were isolated which were deficient in type ii (ca2(+)-dependent) haemolysin activity (hly-). some of the hly- mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. comparison of cell-free culture supernatant from the parent strain and some hly- mutants by sds-page and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kda. two hly- mutants which ... | 1991 | 2033378 |
| lysis of bovine platelets by pasteurella haemolytica leukotoxin. | pasteurella haemolytica a1 culture supernatants caused rapid cytolysis (less than 5 minutes) of isolated bovine platelets as measured by leakage of the cytoplasmic enzyme lactate dehydrogenase (ld). the platelet lytic factor had several features similar to p haemolytica leukotoxin. like p haemolytica leukotoxin, the platelet lytic factor was produced by p haemolytica during logarithmic growth phase, was heat-labile, and was active against target cells (platelets) from ruminant species (cattle an ... | 1991 | 2035922 |
| telescoping plugged catheter. an unusual way of diagnosing pasteurella multocida pneumonia. | we report a case of pasteurella multocida pneumonia and its unusual clinical presentation. we also discuss the rarity of its diagnosis by tpc specimens and the delay in both an adequate diagnosis and treatment. | 1991 | 2036840 |
| comparison and analysis of the nucleotide sequences of pilin genes from haemophilus influenzae type b strains eagan and m43. | previous studies have demonstrated antigenic differences among the pili expressed by various strains of haemophilus influenzae type b (hib). in order to understand the molecular basis for these differences, the structural gene for pilin was cloned from hib strain eagan (p+) and the nucleotide sequence was compared to those of strains m43 (p+) and 770235 b0f+, which had been previously determined. the pilin gene of hib strain eagan (p+) had a 648-bp open reading frame that encoded a 20-amino-acid ... | 1991 | 2037360 |
| interaction of bovine neutrophils in pasteurella haemolytica mediated damage to pulmonary endothelial cells. | the purpose of these studies was to determine mechanisms of pulmonary tissue damage mediated by pasteurella haemolytica and interaction with bovine neutrophils. bovine pulmonary artery endothelial cell monolayers were treated with various combinations of p. haemolytica factors including bacterial culture supernatant (cs) and purified lps, with and without bovine neutrophils. damage to endothelial cells was monitored by 51cr release, cell detachment rate, and morphological changes. at 5 h post-tr ... | 1991 | 2038823 |
| [gyrase inhibitors, a new class of therapeutic drugs]. | fluorinated inhibitors of gyrase open a new field in chemotherapy. for veterinary purposes one drug, enrofloxacin (baytril), has been registered hitherto in germany. well known disadvantages of the old gyrase blockers (e.g. nalidixic acid and others) have been their limited clinical application (compounds were useful only for therapy of infections of kidney cavities) and their deficits in antimicrobial efficacy regarding gram-positive bacteria, pseudomonas and mycoplasmas. fluorinated 4-quinolon ... | 1991 | 2048102 |
| cloning and expression of a 30 kda surface antigen of pasteurella haemolytica. | pasteurella haemolytica biotype a serotype 1 is the principal etiologic agent of bovine pneumonic pasteurellosis. a clear understanding of the pathogenesis of this disease and the mechanisms of resistance to it has been limited by a lack of information on the important antigens of the organisms. using recombinant dna techniques we have cloned a segment of dna from p. haemolytica a1 that encodes three proteins of 28, 30, and 32 kda. two of these proteins, 30 and 28 kda, react strongly on a wester ... | 1991 | 2048283 |
| pasteurella multocida peritonitis in an hiv-positive patient on continuous cycling peritoneal dialysis. | pasteurella multocida has been reported only once previously as a cause of peritonitis in a patient undergoing chronic peritoneal dialysis. the present report describes findings associated with a case of p. multocida peritonitis in a human immunodeficiency virus (hiv)-positive patient in which renal replacement therapy consisted of continuous cycling peritoneal dialysis. to our knowledge this is the first report of this unique infection in an hiv-positive end-stage renal disease patient. in addi ... | 1991 | 2048580 |
| dermonecrotic toxin production by strains of pasteurella multocida isolated from man. | ninety-four clinical isolates of pasteurella multocida of human origin were tested for dermonecrotic toxin (dnt) production by three methods: dermonecrotic test in guinea-pigs, vero cell culture cytotoxicity and elisa. the strains were isolated from patients living in a rural area with widespread intensive pig breeding. six strains were found to be toxigenic by the three tests. a major protein band of mr 145 kda corresponding to dnt on immunoblots was demonstrated in extracts from these strains. ... | 1991 | 2056517 |
| [oligoarthritis in pasteurella multocida inoculation pasteurellosis]. | 1991 | 2057694 | |
| vaccine containing iron-regulated proteins of pasteurella haemolytica a2 enhances protection against experimental pasteurellosis in lambs. | a vaccine containing sodium salicylate extract (sse) of pasteurella haemolytica a2 cells grown in a medium chemically depleted of available iron by the addition of alpha alpha dipyridyl to induce iron-regulated proteins (irps) conferred protection to specific pathogen-free (spf) lambs exposed to an aerosol of p. haemolytica a2. the disease score in these lambs was significantly lower (p less than 0.005) than those in unvaccinated lambs or in lambs immunized with sse prepared from cells grown in ... | 1991 | 2058262 |
| experimental pneumonia in mice produced by combined administration of bordetella parapertussis and pasteurella haemolytica isolated from sheep. | one-hundred-and-thirty-seven, 3-week-old, swiss mice were inoculated intranasally with bordetella parapertussis and pasteurella haemolytica which had been isolated from naturally occurring cases of chronic non-progressive pneumonia in sheep. the combined administration produced a significantly more severe bronchopneumonia which occurred earlier, persisted for a longer period and involved a higher percentage of mice than that which was produced with b. parapertussis or p. haemolytica alone. these ... | 1991 | 2061424 |
| activation of escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation. | haemolysin secreted by pathogenic escherichia coli binds to mammalian cell membranes, disrupting cellular activities and lysing cells by pore-formation. it is synthesized as nontoxic prohaemolysin (prohlya), which is activated intracellularly by a mechanism dependent on the cosynthesized hlyc. haemolysin is one of a family of membrane-targeted toxins, including the leukotoxins of pasteurella and actinobacillus and the bifunctional adenylate cyclase haemolysin of bordetella pertussis, which requi ... | 1991 | 2062368 |
| immunity induced in rats vaccinated with toxoid prepared from heat-labile toxin produced by pasteurella multocida serogroup d. | rats were vaccinated with a toxoid (d-toxoid) prepared from purified heat-labile toxin (d-toxin) produced by pasteurella multocida serogroup d. vaccination of rats with d-toxoid prevented death and other effects of d-toxin (hepatic necrosis, development of elevated leukocyte counts, lymphopenia, neutrophilia, and elevated complement titers) that occurred in phosphate buffered saline (pbs)-vaccinated control rats. | 1991 | 2063548 |
| chronic upper respiratory tract disease of free-ranging desert tortoises (xerobates agassizii). | seventeen desert tortoises, xerobates agassizii, with upper respiratory tract disease were examined; thirteen were euthanatized for necropsy. four normal control desert tortoises from a clinically healthy population were similarly evaluated. hemoglobin and phosphorus values were significantly (p less than or equal to 0.05) lower and serum sodium, urea, sgot, and cholesterol values were significantly higher in ill tortoises compared to controls. no significant differences in concentrations of ser ... | 1991 | 2067052 |
| pasteurella haemolytica is highly sensitive to ultraviolet irradiation. | the response of pasteurella haemolytica to ultraviolet irradiation was determined. the results for survival show that p. haemolytica is very sensitive to uv-irradiation. this uv-sensitivity is similar to e. coli strains defective in uv repair mechanism(s). analysis of the distribution of tca insoluble versus tca soluble [3h]thymine dimers in uv-irradiated dna of p. haemolytica during a 2-h post-irradiation period indicates that the bacterium is deficient in an excision-repair system. these data ... | 1991 | 2067555 |
| rapid development of shock following cat scratch injury in a previously fit middle aged woman. | 1991 | 2071921 | |
| the susceptibility of in vivo-grown pasteurella haemolytica to ovine defence mechanisms in vitro. | pasteurella haemolytica organisms grown in vivo were examined for their susceptibility to ovine immune mechanisms in vitro. compared with in vitro grown organisms they were less susceptible to opsonophagocytosis and, in contrast, susceptible to complement-dependent killing in the absence of exogenous antibody. these differences were not associated with phenotypic changes in the surface of the bacterial cell. however, overproduction and de novo synthesis of proteins was observed in in vivo grown ... | 1990 | 2073409 |
| increased susceptibility to pasteurella haemolytica in lambs infected with bovine respiratory syncytial virus. | groups of conventionally reared, 6- to 8-week-old lambs were inoculated intranasally and intratracheally with either bovine respiratory syncytial virus (brsv) or brsv followed by pasteurella haemolytica 6 days later or with p. haemolytica alone. lambs infected with p. haemolytica 6 days after experimental infection with brsv had significantly higher disease scores, greater magnitudes of fever, and higher mortality rates than those infected with brsv alone or with p. haemolytica alone (p less tha ... | 1990 | 2079556 |
| time and temperature dependence of granulocyte damage by leucotoxic supernatants from pasteurella haemolytica a1. | bacterial exotoxins may contribute to the pathogenic potential of micro-organisms through interactions with cells of the host defence system as well as by directly damaging host tissue. the present studies were designed to explore mechanisms of interaction between bovine granulocytes and the leucotoxin produced by pasteurella haemolytica, a major cause of bovine respiratory disease. leucotoxin-containing supernatant from p. haemolytica a1 caused rapid cell death in isolated bovine granulocytes t ... | 1990 | 2079620 |
| deletion analysis resolves cell-binding and lytic domains of the pasteurella leukotoxin. | a series of internal deletions in the lkta gene of pasteurella haemolytica has been constructed. all of the deletions eliminated the lytic activity of the leukotoxin towards the bovine lymphoma cell line, bl-3. deletions removing segments of the amino-proximal hydrophobic region, which is thought to constitute an essential membrane-spanning domain, were found to agglutinate bl-3 cells. agglutination was similar to lysis by the wild-type toxin in that it was dependent upon the presence of calcium ... | 1990 | 2082150 |
| distribution of glycoprotease activity and the glycoprotease gene among serotypes of pasteurella haemolytica. | 1990 | 2083719 | |
| immunity to pasteurella multocida in protein-deficient chickens. | studies were conducted to determine the effects of dietary protein restriction on the humoral immunity (hi) and cell-mediated immunity (cmi) of chickens. new hampshire chickens were separated into two dietary treatment groups: basal, containing 3,200 kcal/kg and 21% protein; or protein restricted (pr), containing 3,200 kcal/kg and 7% protein. in studies involving hi, half of the birds in each dietary treatment were vaccinated against fowl cholera at 4 and 8 wk of age. blood samples were collecte ... | 1990 | 2084673 |
| immunoperoxidase evaluation of the relationship between necrotic lesions and causative bacteria in lungs of calves with naturally acquired pneumonia. | an immunoperoxidase technique was used to study the relationship between the necrotic lesions and causative bacteria found in lungs of 53 calves that had naturally acquired pneumonia. four types of necrotic lesions were identified on the basis of morphologic characteristics as follows: type 1 had coagulation necrosis surrounded by a dense zone of numerous degenerated leukocytes; type 2 was similar to type 1, but the central area of the lesions was severely affected, had no alveolar architecture ... | 1990 | 2085224 |
| pasteurella multocida infective endocarditis: a case report. | a 17-year-old man presented with acute febrile illness with jaundice, embolic skin lesion, heart murmur, renal insufficiency and abnormal csf. pasteurella multocida was isolated from blood cultures. in spite of adequate antibiotic treatment for endocarditis of the mitral valve, he developed a fatal ruptured cerebral mycotic aneurysm. post mortem examination revealed an atrial septal defect, vegetation at the anterior mitral leaflet, intraventricular, subarachnoid and intracerebral hemorrhage. | 1990 | 2086720 |
| the efficacy of danofloxacin in the therapy of pneumonia associated with pasteurella species in housed calves. | the efficacy of danofloxacin, a novel third generation fluoroquinolone, was assessed in the treatment of pneumonia in housed calves on three farms in the fdr and italy. seventy three calves with clinical signs of acute pneumonia and rectal temperatures greater than 40 degrees c were treated with danofloxacin at a dose rate of 1.25 mg/kg for three or five days depending on response to treatment. the response in these calves was compared to that obtained in 77 calves treated with trimethoprim/sulp ... | 1990 | 2088707 |
| [infection caused by pasteurella multocida after a lion bite]. | 1990 | 2090239 | |
| antimicrobial susceptibility of pasteurella multocida type d from missouri swine. | 1990 | 2090275 | |
| [septic arthritis due to pasteurella multocida]. | 1990 | 2091119 | |
| monoclonal antibody in the identification of haemophilus somnus. | electrophoretic comparisons of outer membrane proteins of haemophilus somnus isolates revealed 2 major protein bands (46 and 14 kilodaltons [kd]) common to all isolates tested. a monoclonal antibody raised against h. somnus reacted to the 46-kd band. coagglutination tests were performed using a monoclonal antibody coagglutination assay. the monoclonal reagent was produced by incubating cowan strain staphylococcus aureus suspension, used as a source of crude protein a, with mouse ascitic fluid mo ... | 1990 | 2094432 |
| immunopathological changes in mice caused by bordetella bronchiseptica and pasteurella multocida. | the immunopathological changes induced by toxigenic pasteurella multocida, toxigenic phase i bordetella bronchiseptica and its phase iii variant were studied. four groups of mice, each containing 21 animals, were inoculated intravenously with sublethal doses of b. bronchiseptica as follows: group 1: phase i toxigenic b. bronchiseptica whole-cell suspension (wcs); group 2: phase iii b. bronchiseptica wcs; group 3: phase i b. bronchiseptica sonicated extract (se); and group 4: phase iii b. bronchi ... | 1990 | 2099605 |
| the pathology of experimental respiratory infection with pasteurella multocida and bordetella bronchiseptica in rabbits. | groups of female new zealand white rabbits, 8-10 weeks old, were inoculated intranasally with three different pasteurella multocida serotypes (a:3, a:4 and a:12) or one of three bordetella bronchiseptica strains of rabbit origin. seven out of 18 rabbits died of experimental infection with p. multocida. b. bronchiseptica killed 3 out of the 8 animals inoculated with it. deaths occurred between 3 and 6 days postinoculation (pi). in the rabbits that died of p. multocida inoculation, necropsy and hi ... | 1990 | 2099606 |
| survey of actinobacillus (haemophilus) pleuropneumoniae infection in swine by different methods. | lung and serum samples from pigs that died or were emergency-slaughtered in a pooled, conventional fattening herd were examined to survey actinobacillus pleuro-pneumoniae infection and to compare the sensitivity of different testing methods. a total of 110 lungs were used for cultural isolation of the agent and direct immunofluorescence (if) of impression smears. boiled lung suspensions were tested by coagglutination (co-a) and agar gel precipitation (agp). eighty-seven sera were tested along wi ... | 1990 | 2099609 |
| demonstration of pasteurella multocida type 6:b (b:2) in formalin-fixed paraffin-embedded tissues of buffaloes by the peroxidase anti-peroxidase (pap) technique. | 1990 | 2099627 | |
| [nonspecific immunostimulating activity of bordetella bronchiseptica bacterin in tests on mice and calves]. | three experimental models were used to verify the effectiveness of immunostimulant preparations of microbial origin. of the four microbial species, used in the tests with the stimulation of mice against subsequent infection with the virulent strain of pasteurella multocida, the highest resistance-increasing power was recorded in bordetella bronchiseptica. there were large differences in stimulation activity between its strains, ranging from those with a low activity up to strain 6229, which incr ... | 1990 | 2100431 |
| pasteurella multocida infections in mice with reference to haemorrhagic septicaemia in cattle and buffalo. | haemorrhagic septicaemia (hs) is an infectious disease of cattle and buffalo caused by particular serotypes of pasteurella multocida and is one of the most economically important livestock diseases in south-east asia. while hs has been recognized for many years, very little is understood about the disease, primarily because of the expense of cattle and a lack of suitable animal models. the suitability of using mice to study hs was assessed using parameters such as the critical pathogenic dose, k ... | 1990 | 2108081 |
| effects of haemorrhagic septicaemia vaccination and levamisole administration on the humoral response in cross-bred calves. | levamisole administered by oral, subcutaneous or transdermal routes following vaccination against pasteurella multocida enhanced the humoral response in cross-bred calves. the group that received levamisole subcutaneously elicited highly significant (p less than 0.01) antibody titres during the primary humoral response in comparison with groups that received levamisole orally or transdermally. during the secondary response in the same experiment, levamisole administered subcutaneously and transd ... | 1990 | 2108257 |
| pasteurella multocida otitis media complicated by facial palsy. | 1990 | 2108421 | |
| receptors for transferrin in pathogenic bacteria are specific for the host's protein. | transferrin receptors detected by a solid-phase binding assay were shown to be specific for the host's transferrin in the representative bacterial pathogens neisseria meningitidis (human), pasteurella haemolytica (bovine), and actinobacillus pleuropneumoniae (porcine). consistent with the receptor specificity, iron-deficient bacteria were only capable of utilizing transferrin from the host as a source of iron for growth. | 1990 | 2110858 |
| atypical pasteurella strains producing a toxin similar to the dermonecrotic toxin of pasteurella multocida subspecies multocida. | this paper is the first report of the production of a dermonecrotic toxin by pasteurella strains that do not belong to the species pasteurella multocida subspecies multocida. four strains, isolated from cattle with atrophic rhinitis, were characterised phenotypically. the strains were related to pasteurellaceae, but their taxonomic position remained unclear. the strains produced a toxin that caused a haemorrhagic dermonecrosis in guinea pigs and was lethal to mice. both effects were neutralised ... | 1990 | 2111955 |
| field use of live haemorrhagic septicaemia vaccine. | 1990 | 2116692 | |
| plasma disposition and tolerance of aditoprim in horses after single intravenous injection. | aditoprim, a broad spectrum antimicrobial agent acting as a reversible dihydrofolate reductase inhibitor, was intravenously injected into four 12 to 24-year old horses at a dosage of 5 mg/kg b. w. blood samples were collected over a 48-hour period after drug injection, and the separated plasma samples were assayed for aditoprim by high performance liquid chromatography. the body temperature, heart rate, respiration rate, and behaviour were recorded during the experiment. the bilirubin and urea c ... | 1990 | 2116704 |
| multivariate analysis of quantitative chemical and enzymic characterization data in classification of actinobacillus, haemophilus and pasteurella spp. | chemotaxonomic data for strains of actinobacillus, haemophilus and pasteurella spp. were analysed using three multivariate statistical strategies: principal components, partial least squares discriminant, and soft independent modelling of class analogy. the species comprised actinobacillus actinomycetemcomitans. haemophilus aphrophilus, h. paraphrophilus, h. influenzae, pasteurella multocida, p. haemolytica and p. ureae. strains were characterized by cell sugar and fatty acid composition, lysis ... | 1990 | 2118166 |
| persistence of the carrier status in haemorrhagic septicaemia (pasteurella multocida serotype 6:b infection) in buffaloes. | fifty-seven young buffaloes were experimentally infected or naturally exposed to haemorrhagic septicaemia (hs). of these animals 32 became immune carriers. they were observed in groups for periods up to 360 days to monitor the appearance of pasteurellae in the nasopharynx and antibody status. in most animals pasteurellae appeared in the nasopharynx for a short period initially and disappeared. the organism reappeared intermittently and the longest observed period of reappearance was 215 days aft ... | 1990 | 2120825 |
| pasteurella haemolytica lipopolysaccharide-induced arachidonic acid release from and neutrophil adherence to bovine pulmonary artery endothelial cells. | bovine pulmonary artery endothelial cells (bpaec) were labeled with 3h-arachidonic acid. exposure of the labeled bpaec to pasteurella haemolytica lipopolysaccharide (lps) resulted in a time- and dose-dependent release of radioactivity. the release was inhibited by 5 mm indomethacin, but inhibition was not caused by less than or equal to 500 microm indomethacin or hydrocortisone, which suggests that the release was caused primarily by a mechanism other than cyclooxygenase or phospholipase a2 meta ... | 1990 | 2122778 |
| the effect of stress on host defense system and on lung damage in calves experimentally infected with pasteurella haemolytica type a1. | this paper describes the influence of stress, immediately before a massive inoculation of a log-phase p. haemolytica a1 culture in brain-heart-infusion broth by fiberoptic bronchoscopy in 5 months old conventional calves. differences in phagocytic cell function and numbers, lung function and lung tissue damage between stressed and non-stressed, infected calves were studied. compared with the non-stressed calves before infection, the superoxide generation by blood polymorphonuclear leucocytes of ... | 1990 | 2123055 |
| pasteurella multocida osteomyelitis of the cervical spine in a patient on chronic haemodialysis. | osteomyelitis due to pasteurella multocida has been frequently documented but virtually all previous cases have resulted from direct inoculation of the organism or contiguous spread of local infection, following animal bites or scratches. infections often occur in patients with serious underlying illnesses. haematogenous osteomyelitis due to p multocida has very rarely been reported particularly in patients with chronic renal failure. we describe a patient on chronic haemodialysis who developed ... | 1990 | 2124004 |
| rapid identification of pasteurella multocida organisms responsible for haemorrhagic septicaemia using an enzyme-linked immunosorbent assay. | haemorrhagic septicaemia (hs) is caused by specific serotypes of pasteurella multocida and is one of the major economic diseases of cattle and buffalo in south east asia. definitive diagnosis of the disease-causing organism with the available methods is labour intensive and not totally reliable, consequently, an elisa system to identify p multocida organisms which cause hs was developed. one hundred and twenty-four p multocida isolates were tested, 58 were type strains and 66 were field isolates ... | 1990 | 2125138 |
| in vitro activity of bay v 3522, a new oral cephalosporin. | the in vitro activity of bay v 3522 was compared with the activities of cephalexin, cefaclor, cefuroxime, cefixime, and amoxicillin-clavulanate. mics (in micrograms/ml) of bay v 3522 were as follows: staphylococcus spp. (except for oxacillin-resistant strains), 0.13 to 1; streptococcus spp. (except for some viridans group streptococci), less than or equal to 0.015 to 0.25; enterococcus faecalis, 2 to 8; other enterococci, 0.5 to greater than 32; beta-lactamase-negative haemophilus influenzae and ... | 1990 | 2126697 |
| the airborne survival of pasteurella haemolytica and its deposition in and clearance from the mouse lung. | pasteurella haemolytica a1 was aerosolised by a collison nebuliser in a henderson apparatus and its survival in air was measured. the organism was fragile in aerosol and survived best at high humidity and warm temperature. mice were exposed to the aerosol and clearance from the lung measured. deposition in the mouse lung showed a good linear correlation with bacterial concentration in the spray suspension fluid. clearance from the lung was rapid over 24 h although some bacteria could be detected ... | 1990 | 2138372 |
| treatment of experimental pasteurellosis in lambs with clavulanic acid and amoxycillin. | 1990 | 2140474 | |
| distribution of pasteurella haemolytica in the respiratory tracts of carrier calves and those subsequently infected experimentally with dictyocaulus viviparus. | distribution of pasteurella haemolytica in the respiratory tracts of calves with no apparent clinical signs of illness and those infected experimentally with dictyocaulus viviparus was determined so as to define carrier sites for this organism. the calves had been positive by nasopharyngeal swab for either p haemolytica a2 or a1 for at least two months or for over a month, respectively, before slaughter. p haemolytica a1 was acquired following horizontal spread from other infected calves. it was ... | 1990 | 2141697 |
| bacterial adhesion to mucosal surfaces with special reference to pasteurella multocida isolates from atrophic rhinitis. | adhesion to mucosal cells is an important virulence attribute of bacterial pathogens colonizing these sites. bacteria of the upper respiratory system, such as members of the genus bordetella, have well-defined adhesins. the main adhesin of b. pertussis is the filamentous hemagglutinin which can be used by other bacteria for attachment. the main adhesin of b. bronchiseptica is the bovine erythrocyte hemagglutinin. in both bordetella species the presence of fimbriae does not appear critical to adh ... | 1990 | 2141799 |
| pulmonary lesions induced by a pasteurella haemolytica cytotoxin preparation in calves. | intrabronchial instillation of a pasteurella haemolytica type a1 crude cytotoxin preparation in calves resulted in pulmonary gross and microscopic lesions comparable to spontaneous and experimental pasteurellosis. in the acute stage of the lesion electronmicroscopy revealed intravascular accumulation, degeneration and fragmentation of leukocytes in the interalveolar septa. secondary thrombus formation and increased vascular permeability resulted in alveolar flooding, fibrin deposition, extravasa ... | 1990 | 2143346 |
| [the effect of local and systemic immunization of suckling pigs on bronchoalveolar clearance and on intrabronchial infections with pasteurella multocida]. | in vivo investigations of bactericidal bronchoalveolar clearance of weaning pigs locally or systemically immunized with pasteurella antigens exhibited clearly distinctive results, as early as 3 days after the last immunization. they differed from controls by significant increase in the elimination of the homologous pasteurella multocida a wild strain. that increase in the clearance remains for 21 days and is specific only to the immunized capsular type. there are no relations of this result to t ... | 1990 | 2143646 |
| clinical and pathological effects of the dermonecrotic toxin of bordetella bronchiseptica and pasteurella multocida in specific-pathogen-free piglets. | the role of dermonecrotic toxin (dnt) of bordetella bronchiseptica and pasteurella multocida, purified by repeated chromatography in sephacryl s-200 gel, in the pathogenesis of atrophic rhinitis (ar) of swine was studied bacteriologically, clinically and pathologically. two-week-old specific pathogen-free (spf) piglets were parenterally treated with 30 micrograms of dnt 3 times at 2-day interval and 7-week-old piglets were treated with 15 micrograms of dnt twice a week for 5 weeks. in 2- to 3-we ... | 1990 | 2144027 |
| the influence of growth conditions of pasteurella multocida on its ability to colonise the nasal mucosa of spf piglets. | colonisation of type d pasteurella multocida was studied in five groups of seven spf piglets each. piglets of group 1 were kept together with seven 5-week-old piglets obtained from a large herd infected with toxigenic p. multocida for 16 weeks (contact infection). these piglets were made free from toxigenic bordetella bronchiseptica by local immunisation. piglets of group 2 were inoculated with 5 x 10(7) colony-forming units (cfu) of p. multocida washed from the nasal mucosa of piglets free from ... | 1990 | 2145686 |
| significance of v-factor dependency in the taxonomy of haemophilus species and related organisms. | 1990 | 2145965 | |
| colonisation by pasteurella multocida in atrophic rhinitis of pigs and immunity to the osteolytic toxin. | gnotobiotic pig antisera to purified toxoid from a capsule type a or d strain of pasteurella multocida contained large quantities of antitoxin but comparatively little antibody to a crude lysate of p. multocida. these sera given intraperitoneally to further pigs were almost completely protective against turbinate atrophy after intranasal inoculation of dilute acetic acid and infection with type d toxigenic p. multocida. in contrast, antisera to a crude lysate or bacterin of toxigenic p. multocid ... | 1990 | 2149226 |
| characterization of pasteurella multocida mutants of low virulence. | ten temperature-sensitive mutants of the clemson university (cu) vaccine strain of pasteurella multocida have been developed and were characterized by phenotypic attributes such as carbohydrate fermentation, antibiotic resistance, and membrane protein profiles. some mutants were found to have lost the ability to utilize some substrates, notably xylose and gluconate, whereas others were able to ferment additional carbohydrates such as arabinose and rhamnose. cu was found to be resistant to sulfis ... | 1990 | 2149262 |
| [further development of p. multocida vaccines for swine]. | 149 strains with antigen fractions of both a and d type could be found out of 446 p. multocida field strains of porcine origin. most of them are producing the dermonecrotizing toxin. these a/d strains proved to be virulent in mice and piglets as well. in mice, the vaccination with one of the most virulent and immunogenic a/d strains, inactivated and a1(oh)3 adsorbed caused an immunity against challenge infections with p. multocida of types a, d and a/d. this effect could be confirmed on spf pigl ... | 1990 | 2150307 |
| pasteurella multocida toxin: potent mitogen for cultured fibroblasts. | native pasteurella multocida toxin (pmt) is shown to be an extremely potent mitogen for swiss 3t3 fibroblasts. half-maximal stimulation of dna synthesis was obtained at concentrations of 1 and 2 pm for recombinant pmt (rpmt) and pmt, respectively. the degree of rpmt-induced dna synthesis was comparable to that elicited by 10% fetal bovine serum and, moreover, was observed in the complete absence of other factors. cell proliferation was also enhanced by rpmt. the toxin was also a potent mitogen f ... | 1990 | 2153282 |
| dna fingerprinting for differentiation of field isolates from reference vaccine strains of pasteurella multocida in turkeys. | the genomes from field isolates of pasteurella multocida in turkeys and those of p multocida reference cu and m9 vaccine strains were analyzed and compared after cleavage with restriction endonucleases. the electrophoretic profiles obtained with dna fragments from field isolates and vaccine strains of the same serotype were characteristic and reproducible. these features indicated the existence of differences among the isolates of the same serotype that cannot currently be detected, using availa ... | 1990 | 2154149 |
| tn7 inserts in both orientations at a single chromosomal location and apparently forms cointegrates in pasteurella multocida. | pasteurella multocida transconjugants isolated after mating with escherichia coli strains that carry one or the other of two tn7-containing suicide plasmids, prktv5 and puw964 (prktv5::tn5), were analysed. these plasmids have the cole1 replication origin and were thus expected to deliver transposons but not be maintained as free replicons in pasteurella. five out of six transconjugants selected for acquisition of tn7 from e. coli (prktv5) had simple insertions of the transposon, in either orient ... | 1990 | 2157128 |
| naturally acquired pasteurella multocida infection in rabbits: immunological aspects. | natural infection with p. multocida in new zealand white rabbits was followed in 2 to 3 weeks by development of immunoglobulin (ig)m and igg serum antibodies. the igm response peaked and returned to lower levels within several weeks after infection, whereas the igg response progressively increased and remained elevated. | 1990 | 2162985 |
| a group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in ontario feedlot calves. | the associations, at the group level, between serological titer to pasteurella haemolytica surface antigens (ph), pasteurella haemolytica cytotoxin (ph-cytox), infectious bovine rhinotracheitis virus (ibrv), bovine virus diarrhea virus (bvdv), parainfluenza-3 virus (piv3), respiratory syncytial virus (rsv), mycoplasma dispar (md), m. bovis (mb), and respiratory disease treatment rates, relapse rates, and 28 day weight gains were investigated in 14 groups of calves entering two feedlots during ye ... | 1990 | 2165846 |
| protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves. | formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ... | 1990 | 2168606 |
| serological surveillance for antibodies against different avian infectious agents on turkey flocks naturally infected with turkey rhinotracheitis. | turkey rhinotracheitis (trt) in the state of baden-württemberg, germany appears to have become endemic affecting all farms and every new restocking. in 5 meat turkey flocks, serological surveillance for antibodies to trt-, ib- and ibd-viruses, as well as to pasteurella multocida were carried out using elisa tests. furthermore, sera were examined for the presence of antibodies to avian adenovirus (celo virus), reo- and paramyxo-viruses 1 and 3. the birds were bled at 4 week intervals starting at ... | 1990 | 2168612 |
| [serological survey on newcastle and gumboro diseases, pasteurellosis and pullorosis in local hens in niger]. | a serological survey on avian pathology in a village area was carried out in niger. it allowed to determine the incidence of pullorosis (47%), pasteurellosis (48%), avian bursal disease (47%) and newcastle disease (14% in non vaccinated and 63% in vaccinated animals). | 1990 | 2175925 |
| pasteurella haemolytica as a co-pathogen in pullets and laying hens. | pasteurella haemolytica was isolated from internal organs of laying hens and pullets of four flocks in which five incidents of increased mortality had occurred. there was inflammation of the trachea in all cases, and infectious laryngotracheitis virus was identified in three of the incidents. rapid response to antimicrobial therapy was seen in two out of three outbreaks in the pullets. p. haemolytica was regarded as a significant secondary invader in these cases. | 1990 | 2177972 |
| monoclonal antibodies against surface antigens of pasteurella multocida strain p-1059. | four monoclonal antibodies (mabs) were developed against serotype 3:a, p-1059 strain of pasteurella multocida. enzyme-linked immunosorbent assays were used to screen those hybridomas producing antibodies to either a surface protective (2.5 s) or lipopolysaccharide (lps) antigen. mabs 6ee11, d7h10, e11e3, and c11h2 were positive against 2.5 s antigen, and two of them, e11e3 and c11h2, were positive for the lps antigen. mabs 6ee11 and d7h10 reacted with a major protein band of molecular weight of ... | 1990 | 2181995 |
| secretion and expression of the pasteurella haemolytica leukotoxin. | the pasteurella haemolytica leukotoxin gene cluster (lktcabd) is homologous to the escherichia coli hemolysin locus (hlycabd). since the cloned leukotoxin (lkta) is not secreted from e. coli cells, a heteroplasmid complementation system was developed that permits secretion of the leukotoxin from cells expressing the hemolysin transport proteins hlyb and hlyd. we observed that the secreted leukotoxin protein had weak hemolytic activity when activated by either the hlyc or lktc proteins and that l ... | 1990 | 2185213 |
| possession of identical nonconjugative plasmids by different isolates of pasteurella multocida does not imply clonality. | experiments were performed to test the hypothesis that possession of the same nonconjugative r plasmid by different isolates of pasteurella multocida implied that they were of the same clone. seven isolates of p. multocida were studied, two possessed an identical nonconjugative r plasmid (pvm109), four possessed another (pvm110), and one isolate possessed a nonconjugative r plasmid related to pvm110. phenotypic and genotypic characteristics of the isolates were determined and compared. isolates ... | 1990 | 2186566 |
| investigators relate experience with bovine somatotropin. | 1990 | 2189847 | |
| immunohistochemical localization of pasteurella haemolytica a1-derived endotoxin, leukotoxin, and capsular polysaccharide in experimental bovine pasteurella pneumonia. | six, 5- to 10-week-old male holstein calves were inoculated intratracheally with 5 x 10(9) logarithmic growth phase pasteurella haemolytica biotype a serotype 1 (a1). immunohistochemical techniques in conjunction with the use of monoclonal antibodies directed against p. haemolytica a1-derived lipopolysaccharide (lps), capsular polysaccharide, and a polyclonal rabbit anti-leukotoxin antibody were used to localize their respective antigens in tissue sections of pneumonic lung at the light and elec ... | 1990 | 2191489 |
| pasteurella multocida meningitis in an adult: case report and review. | pasteurella multocida is a rare cause of adult meningitis. close animal contact prior to onset of illness is frequent and represents the usual mode of introduction of the organism. in reports of a total of 21 cases of p. multocida meningitis in adults (this case report and 20 described previously in the english-language literature), 18 researchers commented on the occurrence of animal contact: two cases (11%) involved cat bite, 13 (72%) involved animal contact without bite, and three (17%) occur ... | 1990 | 2193351 |
| socioeconomic significance of the hap group. | an overview is presented of some members of the hap group important in animal disease. an attempt has been made to highlight the economic importance of these diseases and to alert us to the importance of research that will lead to their prevention and control. | 1990 | 2193697 |
| overview of the virulence attributes of the hap-group of bacteria. | the pathogenicity requirements of the hap bacteria include colonization of mucous surfaces, invasion of the host tissues, survival and multiplication in the host, interference with the defences of the host, and damage to the host. for these purposes the bacteria possess adhesion structures, capsular polysaccharides, surface structures such as outer membrane proteins, and lipopolysaccharides. they also secrete extracellular products, including exotoxins. the information available in this context ... | 1990 | 2193698 |
| capsules and virulence in the hap group of bacteria. | many species in the genera haemophilus, actinobacillus, and pasteurella produce serotype-specific, negatively-charged, polysaccharide capsules. in pure form, these capsules are nontoxic, relatively inert biologically, and some are poorly immunogenic. the capsule forms the outer-most surface of the bacterium, and prior to the development of specific antibody, inhibits the bactericidal and opsonic activity of normal serum. in contrast, noncapsulated isogenic mutants are generally avirulent, and ar ... | 1990 | 2193699 |
| molecular characterization of cytotoxins produced by haemophilus, actinobacillus, pasteurella. | the leukotoxin of pasteurella haemolytica has been implicated as one of the major virulence factors contributing to the pathogenesis of bovine pneumonic pasteurellosis. to gain a better understanding of the various biochemical, antigenic and toxigenic properties of the toxin, the genetic determinant (lkt) which encodes the leukotoxin has been cloned from p haemolytica serotype 1. results from the molecular characterization of this determinant showed that four genes are required to synthesize and ... | 1990 | 2193701 |
| interactions of haemophilus-actinobacillus-pasteurella bacteria with phagocytic cells. | the haemophilus-actinobacillus-pasteurella (hap) group of bacteria contains a number of important veterinary and human pathogens. although each species has specific characteristics and host range, most share the general property of being resistant to cellular defense mechanisms. in some cases (e.g. pasteurella multocida, pasteurella haemolytica and actinobacillus pleuropneumoniae) resistance results in part from the presence of an antiphagocytic capsule that protects the bacilli against ingestio ... | 1990 | 2193702 |
| positive and negative aspects of host immune response to haemophilus, actinobacillus and pasteurella. | haemophilus somnus, actinobacillus pleuropneumoniae and pasteurella haemolytica are economically important bacteria with pathogenic characteristics that require us to look further than killed, whole cell bacterins for induction of a protective immune response. a strong immune response is not synonymous with protection and the extreme specificity of the immune response works to our disadvantage when broad protection is needed. detection of animals that are susceptible or immune to infection is im ... | 1990 | 2193703 |
| molecular aspects of the virulence of pasteurella multocida. | molecules important to the virulence of all pasteurella multocida are little known, but much has been learned of isolates causing atrophic rhinitis of pigs. a protein toxin purified from p. multocida or from a recombinant escherichia coli reproduces atrophic rhinitis and leads to a reduction in weight gain; antitoxin is completely protective. toxin is important for p. multocida colonization, especially in the presence of the bordetella bronchiseptica cytotoxin. the p. multocida toxin binds rapid ... | 1990 | 2193704 |
| new diagnostic techniques: a review of the hap group of bacteria. | pasteurella haemolytica, pasteurella multocida, haemophilus somnus, haemophilus parasuis and actinobacillus pleuropneumoniae cause considerable economic loss in the cattle and swine industries. the literature relating to diagnostic procedures for these infections is reviewed. both conventional and newer techniques are appraised in relation to the identification and classification of isolates, and to serological testing. | 1990 | 2193708 |
| haemophilus, actinobacillus, pasteurella: mechanisms of resistance and antibiotic therapy. | clinical isolates of members of the family pasteurellaceae show resistance to drugs used for therapy of common infectious diseases of animals. veterinarians want to use an antimicrobial that is effective against the pathogen, continues to provide therapy for several days, and is not too expensive. resistant bacteria have complicated the problem of selecting the best antibiotics for treatment of livestock. resistance to some antibiotics, such as those which inhibit ribosome function, may be encod ... | 1990 | 2193709 |
| our understanding of the pasteurellaceae. | with the exception of a few consistent pathogens--pasteurella multocida strains of bovine hemorrhagic septicemia and fowl cholera, actinobacillus (haemophilus) pleuropneumoniae, haemophilus aegyptius and haemophilus paragallinarum--members of the family pasteurellaceae are commensal parasites on mucous membranes of vertebrate animals. many have pathogenic potential, which becomes manifest under conditions of immunodeficiency and stress. pathogenesis (except in porcine atrophic rhinitis) depends ... | 1990 | 2193710 |
| homogeneity of characteristics of pasteurella multocida isolated from turkeys and wildlife in california, 1985-88. | five hundred twenty isolates of pasteurella multocida, collected in california from september 1985 to november 1988, were characterized in the laboratory. characteristics examined included serotype, capsular type, biotype (subspecies), and possession of plasmid dna. three hundred thirty-three isolates recovered from turkeys dying from fowl cholera, 88 isolates from liver turkeys in flocks with fowl cholera outbreaks in the recent past, and 99 isolates from wildlife captured on fowl cholera-outbr ... | 1990 | 2196044 |
| specificity of bovine serum antibody to capsular carbohydrate antigens from pasteurella haemolytica. | a more complete understanding of the bovine immune response to antigens of pasteurella haemolytica biotype a, serotype 1, will improve control of bovine respiratory disease (brd). sera were obtained from blood samples of calves as they transited the market system of eastern tennessee and were transported to a feedlot in texas. the clinical histories and performance data were recorded and compared with serologic findings. the calves underwent a natural challenge of brd. serologic and bacteriologi ... | 1990 | 2199487 |
| autoagglutination and the specificity of the indirect fluorescent antibody test applied to the identification of taylorella equigenitalis. | because the first strains of taylorella equigenitalis isolated in the netherlands autoagglutinated, identification was difficult. the source of carbon dioxide to create a carbon dioxide atmosphere for incubation influenced the emulsifiability of these strains. strains were emulsifiable when cultivated in a carbon dioxide incubator (7 per cent carbon dioxide in air), but were autoagglutinable when cultivated in a candle jar, or in a jar with a carbon dioxide system or anaerobic system without the ... | 1990 | 2200093 |
| comparison of dna:dna homology and enzymatic activity between pasteurella haemolytica and related species. | a commercially available microbiological identification system and dna:dna hybridization were used to determine relationships between and within serovars 1-13 of pasteurella haemolytica, and between p haemolytica and p multocida and 4 species of actinobacillus. all serovars of p haemolytica that belonged to biovar a were related with mean dna homology of 78%, whereas all serovars of p haemolytica that belonged to biovar t were related to each other with mean dna homology of 90%. the dna:dna hybr ... | 1990 | 2201228 |
| the complete nucleotide sequence of the pasteurella multocida toxin gene and evidence for a transcriptional repressor, txar. | the osteolytic toxin of pasteurella multocida induces bone resorption in vivo and in vitro (foged et al., 1988; kimman et al., 1987). in this report the toxin-encoding toxa gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal-binding motif. evidence that expression of the toxa gene is repressed at a transcriptional level in escherichia coli is presented. repression could be abolished either by deletion of ... | 1990 | 2201870 |
| specificity of dna probes for the detection of toxigenic pasteurella multocida subsp. multocida strains. | five dna probes directed against different regions of the gene that encodes the dermonecrotic toxin of pasteurella multocida subsp. multocida were examined for their ability to identify toxigenic p. multocida subsp. multocida strains. the specificities of the probes were studied with 96 strains of p. multocida subsp. multocida and 22 strains of 11 other bacterial species. results of colony hybridization assays using these probes indicated that two of the five probes have potential diagnostic val ... | 1990 | 2203816 |
| bovine recombinant granulocyte-macrophage colony-stimulating factor enhancement of bovine neutrophil functions in vitro. | neutrophils were purified from blood of dexamethasone-treated (0.04 mg/kg of body weight) and untreated calves. cells were untreated (controls) or cultured in media containing 5 or 10 ng of bovine recombinant granulocyte-macrophage colony-stimulating factor (rbgm-csf)/ml for 10 to 12 hours before being tested for various functions. dexamethasone treatment of calves decreased luminol-dependent chemiluminescence, decreased phagocytosis of pasteurella multocida and several staphylococcus spp by var ... | 1990 | 2204299 |
| a case of unexpected pasteurella multocida bacteremia. | a case of pasteurella multocida bacteremia in a previously healthy hospital employee is presented. the patient had sustained a scratch from his dog four days prior to being seen in the emergency department with adequate healing and no evidence of localized infection. he presented with an acute febrile illness, and was discharged from the emergency department with a diagnosis of viral syndrome. he was asked to return to the hospital the next day when a bacteriology report of gram negative rods in ... | 1990 | 2212562 |