Publications

Epidemiology of Mycoplasma bovis in Pennsylvania veal calves.	The objective of the study was to estimate the prevalence and incidence of Mycoplasma bovis, a common cause of pneumonia, in veal calves. Using simple random sampling, 252 calves from 4 veal herds located in central Pennsylvania were selected and longitudinally followed for monthly collection of nasal swabs. Bronchial swabs and lung lesions were collected at the slaughterhouse. Nasal, bronchial, and lung lesion swabs were cultured for bacterial respiratory pathogens. Ninety lung lesions were ide ...	2012	22192204
subcutaneous botryomycosis due to bibersteinia trehalosi in a texas longhorn steer.	a 3-year-old texas longhorn steer had a long history of progressive swelling of the soft tissues of the jaw and neck. at necropsy, multifocal to coalescing dermal and subcutaneous pyogranulomas were surrounded by fibrous tissue. microscopically, the pyogranulomas contained aggregates of gram-negative coccobacilli surrounded by splendore-hoeppli material and were separated by bands of fibrovascular tissue (botryomycosis). phylogenetic analysis of multilocus sequence-typing data revealed that the ...	2011	22194356
synthesis of heparosan oligosaccharides by pasteurella multocida pmhs2 single-action transferases.	pasteurella multocida heparosan synthase pmhs2 is a dual action glycosyltransferase that catalyzes the polymerization of heparosan polymers in a non-processive manner. the two pmhs2 single-action transferases, obtained previously by site-directed mutagenesis, have been immobilized on ni(ii)-nitrilotriacetic acid agarose during the purification step. a detailed study of the polymerization process in the presence of non-equal amounts of pmhs2 single-action transferases revealed that the glucuronyl ...	2011	22198719
Pasteurella multocida Infection in a Neonate: Evidence for a Human-to-Human Horizontal Transmission.	Pasteurella multocida is usually transmitted by animal contact but in a significant proportion of cases no animal exposure can be identified. Although vertical transmission has been identified in neonates, horizontal human-to-human spread has not been documented. A case of neonatal sepsis and meningitis resulting from horizontal transmission of P. multocida is described.	2011	22198826
Otariodibacter oris gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from the oral cavity of pinnipeds.	A total of 27 bacterial isolates from California sea lions and a walrus tentatively classified with the family Pasteurellaceae were further characterized by geno- and phenotypic tests. Phylogenetic analysis of partial 16S rRNA and rpoB gene sequences showed that the isolates investigated formed a monophyletic group, tentatively designated Bisgaard taxon 57. According to 16S rRNA gene sequence, the closest related validly named species was Bisgaardia hudsonensis with 95 % similarity and the close ...	2011	22199211
Bactericidal properties of pradofloxacin against veterinary pathogens.	Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, ...	2011	22209121
interaction of bordetella bronchiseptica, pasteurella multocida, and fumonisin b1 in the porcine respiratory tract as studied by computed tomography.	the interaction of bordetella bronchiseptica, toxigenic pasteurella multocida serotype d, and the mycotoxin fumonisin b(1) (fb(1)) was studied. on day 0 of the experiment, 28 artificially reared 3-day-old piglets were divided into 4 groups (n = 7 each): a control group (a), a group fed fb(1) toxin (b), a group infected with the 2 pathogens (c), and a group infected with the 2 pathogens and fed fb(1) toxin (d). the b. bronchiseptica infection [with 10(6) colony-forming units (cfu)/ml] was perform ...	2011	22210993
host response in rabbits to infection with pasteurella multocida serogroup f strains originating from fowl cholera.	although pasteurella multocida serogroup f has been described as an avian-adapted serogroup, it was recently found in rabbit nests in the czech republic. therefore, the ability of 2 avian p. multocida serogroup f strains to induce disease in rabbits was investigated. two groups of 18 pasteurella-free rabbits were intranasally challenged with strains isolated from chickens and turkeys. half of the animals in each challenge group were immunosuppressed using dexamethasone. all of the challenged rab ...	2011	22210996
glutathione biosynthesis in bacteria by bifunctional gshf is driven by a modular structure featuring a novel hybrid atp-grasp fold.	glutathione is an intracellular redox-active tripeptide thiol with a central role in cellular physiology across all kingdoms of life. glutathione biosynthesis has been traditionally viewed as a conserved process relying on the sequential activity of two separate ligases, but recently, an enzyme (gshf) that unifies both necessary reactions in one platform has been identified and characterized in a number of pathogenic and free-living bacteria. here, we report crystal structures of two prototypic ...	2011	22226834
life-threatening respiratory pasteurellosis associated with palliative pet care.	pasteurella multocida is a zoonotic gram-negative cocco-bacillus often associated with soft tissue infections due to dog and cat bites. here we report 3 patients who developed life-threatening p. multocida respiratory tract infections after providing palliative care to their dying pets.	2012	22238163