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crystal structure and immunogenicity of the class c acid phosphatase from pasteurella multocida.pasteurella multocida is a pathogen of veterinary and medical importance. here, we report the 1.85å resolution crystal structure of the class c acid phosphatase from this organism (denoted rpmccap). the structure shows that rpmccap exhibits the same haloacid dehalogenase fold and dimeric assembly as the class c enzyme from haemophilus influenzae. formation of the dimer in solution is demonstrated using analytical ultracentrifugation. the active site is devoid of a magnesium ion due to the presen ...201121371420
pasteurella multocida heddleston serovars 1 and 14 express different lipopolysaccharide structures but share the same lipopolysaccharide biosynthesis outer core locus.pasteurella multocida strains are classified using the heddleston lipopolysaccharide (lps) serotyping scheme into 16 serovars. understanding the structural and genetic basis for this lps typing scheme is important because protection against infections caused by p. multocida is generally considered to be serovar specific. here we show that the serovar 14 type strain p2225 and the serovar 1 strains x73 and vp161 express similar lps structures. however, the serovar 14 lps lacks the terminal phospho ...201121371830
pathology, tissue metalloproteinase transcription and haptoglobin responses in mice after experimental challenge with different isolates of pasteurella multocida obtained from cases of porcine pneumonia.pasteurella multocida is a major cause of porcine pneumonia, but the pathogenesis of the disease is poorly defined. the aim of this study was to further understand the host response to infection by use of a mouse model of p. multocida pneumonia. twenty female mice were divided into four groups (n=5). three groups were infected with one of three isolates of p. multocida isolated from clinical cases of chronic porcine pneumonia with necrotizing, suppurative and non-suppurative lesions, respectivel ...201121388634
penetration of oxytetracycline into the nasal secretions and relationship between nasal secretions and plasma oxytetracycline concentrations after oral and intramuscular administration in healthy pigs.the penetration of oxytetracycline (otc) in plasma and nasal secretions of healthy pigs was evaluated during the first study, in response to oral dose of 20ôçâmg of otc per kg of body weight (bwt) per day as a 400ôçâmg/kg feed medication (nôçâ=ôçâ5) and to intramuscular (i.m.)-administered formulations at 10ôçâmg/kg bwt (nôçâ=ôçâ5), 20ôçâmg/kg bwt (nôçâ=ôçâ5), 40ôçâmg/kg bwt (nôçâ=ôçâ5). concentrations of otc in plasma and nasal secretions were determined by a validated ultra-high performance li ...201121395609
cloning, expression and protective capacity of 37 kda outer membrane protein gene (omph) of pasteurella multocida serotype b:2.the major outer membrane protein (omph) of 4 local malaysian strains of pasteurella multocida serotype b:2 were characterized in comparison to atcc strains. three major peptide bands of mw 26, 32 and 37 kda were characterized using sdspage. two of these fragments, the 32 kda and 37 kda were observed to be more reactive with a mouse polyclonal antiserum in all of the local isolates as well as the atcc strains in a western blot. however, the 32 kda fragment was found to cross react with other gram ...201021399583
molecular basis of macrolide, triamilide, and lincosamide resistance in pasteurella multocida from bovine respiratory disease.the mechanism of macrolide-triamilide resistance in pasteurella multocida has been unknown. during whole-genome sequencing of a multiresistant bovine p. multocida isolate, three new resistance genes, the rrna methylase gene erm(42), the macrolide transporter gene msr(e), and the macrolide phosphotransferase gene mph(e), were detected. the three genes were pcr amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triami ...201121402855
molecular and virulence characteristics of an outer membrane-associated rtx exoprotein in pasteurella pneumotropica.pasteurella pneumotropica is a ubiquitous bacterium that is frequently isolated from laboratory rodents and causes various clinical symptoms in immunodeficient animals. currently two rtx toxins, pnxia and pnxiia, which are similar to hemolysin-like high-molecular-weight exoproteins are known in this species. in this study, we identified and analyzed a further rtx toxin named pnxiiia and the corresponding type i secretion system.201121410992
medical microbiologythe genus pasteurella was originally proposed and described by trevisan in 1887. it consisted of a group of nonmotile, small (0. 7 μm by 0. 5 μm), gram-negative coccobacilli often exhibiting a characteristic type of bipolar staining (fig. 29-1). most members of this genus are associated with severe, life-threatening systemic diseases involving both hemorrhagic pneumonia and septicemia. the first pathogen to be ...199621413268
medical microbiologyall bacteria, both pathogenic and saprophytic, are unicellular organisms that reproduce by binary fission. most bacteria are capable of independent metabolic existence and growth, but species of chlamydia and rickettsia are obligately intracellular organisms. bacterial cells are extremely small and are most conveniently measured in microns (10-6 m). they range in size from large cells such as ...199621413343
haemolytic and cytotoxic activities of the tween 80-extracted putative haemolysin of pasteurella multocida b:2.the objective of this study was to investigate the haemolytic and cytotoxic activity of pasteurella multocida b:2 strains, originally from cases of haemorrhagic septicaemia in cattle. all six p. multocida b:2 strains were non-haemolytic on sheep blood agar (sba) and horse blood agar (hba) when grown aerobically and on sba anaerobically but they were haemolytic on hba when grown anaerobically. no haemolytic activity against horse red blood cells was detected in culture supernates from aerobically ...201121420800
microbial ecology of fish species ongrowing in greek sea farms and their watery environment.the present study focuses on the bacteriological profile of both watery ecosystem and fishes from different north and central west greek fish-farms ongrowing euryhaline fish species. the natural microflora of the fish and the water of their ongrowing units in selected farms were studied for a period of 20 months. the analyzed samples were mainly sea bream (sparus aurata) 61.3% and sea bass (dicentrarchus labrax) 24%. in most of the watery ecosystems coming from the different sampling areas, tota ...201121421068
genetic diversity and associated pathology of pasteurella multocida isolated from porcine pneumonia.pasteurella multocida is a widespread respiratory pathogen in pigs associated with atrophic rhinitis and contributing to aggravation of the pulmonary lesions. the aims of the present study were to characterize isolates of p. multocida from porcine bronchopneumonia by pulsed-field gel electrophoresis (pfge), pcr based capsular typing and multilocus sequence typing (mlst) and to compare clonal complexes outlined with the type of histological lung lesions to investigate if a correlation between clo ...201121439738
[fatal clarkson syndrome mimicking a septic shock]. 201121440390
[unusual infection--pasteurella canis bacteremia in a child after exposure to rabbit secretions].pasteurella spp. is a natural habitant of the oral flora and digestive tract of various domestic animals. there are several species of pasteurella which can cause disease in humans. the most common species is pmultocida, generally associated with an animal bite. the infection that evolves is usually constricted to the area of the bite. systemic forms of infection are rare and were described in patients with underlying diseases. the authors would like to report on a case of a healthy 21-month-old ...201121449149
plasma concentrations resulting from florfenicol preparations given to pigs in their drinking water.florfenicol administered through the drinking water has been recommended as a metaphylactic antibacterial drug to control outbreaks of respiratory diseases in pigs caused by strains of actinobacillus pleuropneumoniae and pasteurella multocida, yet it is difficult to pinpoint in practice when the drug is given metaphylactically or therapeutically. further, pigs are likely to reject florfenicol-medicated water, and plasma concentrations of the drug are likely to be marginal for diseases caused by ...201121454858
the forgotten giants behind louis pasteur: contributions by the veterinarians toussaint and galtier. 201021466009
molecular identification of bacteria associated with canine periodontal disease.periodontal disease is one of the most common diseases of adult dogs, with up to 80% of animals affected. the aetiology of the disease is poorly studied, although bacteria are known to play a major role. the purpose of this study was to identify the bacteria associated with canine gingivitis and periodontitis and to compare this with the normal oral flora. swabs were obtained from the gingival margin of three dogs with gingivitis and three orally healthy controls, and subgingival plaque was coll ...201121489726
structural modification of sanguinarine and chelerythrine and their antibacterial activity.in this study, five derivatives of sanguinarine (1) and chelerythrine (2) were prepared, with 1 and 2 as starting materials, by reduction, oxidation and nucleophilic addition to the iminium bond c=n(+). the structures of all compounds were elucidated on account of their ms, (1)h-nmr and (13)c-nmr data. the antibacterial activities of all compounds were screened, using staphylococcus aureus, escherichia coli, aeromonas hydrophila and pasteurella multocida as test bacteria. the minimum bacteriosta ...201121491327
influence of a pig respiratory disease on the pharmacokinetic behaviour of amoxicillin after oral ad libitum administration in medicated feed.the pharmacokinetic properties of amoxicillin in healthy and respiratory-diseased pigs were studied, after ad libitum administration of medicated feed. in addition, amoxicillin dose linearity and drug penetration into respiratory tract tissues were evaluated in diseased animals. the respiratory disease involves porcine reproductive and respiratory syndrome virus and bacterial agents such as pasteurella multocida, bordetella bronchiseptica and streptococcus suis. typical clinical signs and gross ...201121492192
demonstration of the metaphylactic use of gamithromycin against bacterial pathogens associated with bovine respiratory disease in a multicentre farm trial.on five commercial cattle rearing sites across europe, a total of 802 young cattle at high risk of developing bovine respiratory disease (brd) associated with the bacterial pathogens mannheimia haemolytica or pasteurella multocida and/or mycoplasma bovis were enrolled into a multicentre, controlled field trial. half were treated with a single dose of gamithromycin at 6 mg/kg bodyweight by subcutaneous injection and half received an injection of a saline placebo as the control. all animals were o ...201121493573
enzymatic placement of 6-o-sulfo groups in heparan sulfate.heparan sulfate is a highly sulfated polysaccharide that exhibits important physiological and pathological functions. the glucosamine residue of heparan sulfate can carry sulfo groups at the 2-n, 3-o, and 6-o positions, leading to diverse polysaccharide structures. 6-o-sulfation at the glucosamine residue contributes to a wide range of biological functions. here, we report a method for controlling the positioning of 6-o-sulfo groups in oligosaccharides. this was achieved by synthesizing oligosac ...201121506605
infectious microorganisms in mice (mus musculus) purchased from commercial pet shops in germany.in this study, we investigated the prevalence of infectious microorganisms (viruses, bacteria, fungi and eukaryotic parasites) in mice from different pet shops in germany; such animals may compromise the hygienic integrity of laboratory animal vivaria if private pet holders act as unintended vectors of infections carried by them. house mice sold as pets or feed specimens were purchased from different pet shops and tested for a comprehensive panel of unwanted microorganisms. we found a number of ...201121508117
molecular epidemiology of pasteurella multocida in dairy and beef calves.the molecular epidemiology of pasteurella multocida has rarely been studied at the farm level in cattle. the aim of this study was to determine whether single or multiple strains of p. multocida tend to exist within farms. molecular characterisation was carried out on isolates obtained from nasal swabs from 105 calves from 32 randomly selected beef and dairy farms located throughout scotland, and from 131 calves from 20 farms in the mayenne region of france, where sampling occurred in response t ...201121511409
pmst2 - a novel pasteurella multocida glycolipid {alpha}2-3-sialyltransferase.pasteurella multocida is a multi-species pathogen that causes diseases in animals and humans. sialyltransferase activity has been detected in multiple pasteurella multocida strains and sialylation has been shown to be important for the pathogenesis of pasteurella multocida. three putative sialyltransferase genes have been identified in pasteurella multocida genomic strain pm70. we have reported previously that a pm0188 gene homolog in p. multocida strain p-1059 (atcc 15742) encodes a multifuncti ...201121515586
association of porcine circovirus type 2 with vascular lesions in porcine pneumonia.the aim of this study was to evaluate the vasculature in porcine circovirus type 2-infected (pcv2-infected) lungs and to identify the pcv2 subtypes involved in porcine pneumonia. pulmonary samples from 140 pigs, 2 weeks to 7 months of age, from 36 hungarian commercial herds with clinical signs of respiratory disease were examined for the presence of respiratory pathogens, with bacterial culture, pathologic evaluation, and immunohistochemistry for pcv2, porcine reproductive respiratory syndrome v ...201121551427
cellular and molecular action of the mitogenic protein-deamidating toxin from pasteurella multocida.the mitogenic toxin from pasteurella multocida (pmt) is a member of the dermonecrotic toxin family, which includes toxins from bordetella, escherichia coli and yersinia. members of the dermonecrotic toxin family modulate g-protein targets in host cells through selective deamidation and/or transglutamination of a critical active site gln residue in the g-protein target, which results in the activation of intrinsic gtpase activity. structural and biochemical data point to the uniqueness of pmt amo ...201121569202
genetic diversity of pasteurella dagmatis as assessed by analysis of the 16s rrna and rpob gene sequences.a total of 16 pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16s rrna and rpob gene sequence comparison. phylogenetic studies based on both genes revealed that the population of p. dagmatis recovered from cats in poland differs markedly from canine strains, constituting a well-separated cluster within pasteurella sensu stricto species group. the isolate from a tiger seems to represent yet another ev ...201121573831
chemoenzymatic synthesis of c8-modified sialic acids and related α2-3- and α2-6-linked sialosides.naturally occurring 8-o-methylated sialic acids, including 8-o-methyl-n-acetylneuraminic acid and 8-o-methyl-n-glycolylneuraminic acid, along with 8-o-methyl-2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid (kdn8me) and 8-deoxy-kdn were synthesized from corresponding 5-o-modified six-carbon monosaccharides and pyruvate using a sialic acid aldolase cloned from pasteurella multocida strain p-1059 (pmnana). in addition, α2-3- and α2-6-linked sialyltrisaccharides containing neu5ac8me and kdn8deox ...201121592790
structural analyses of the core oligosaccharide from the lipopolysaccharide of bovine and ovine strains of mannheimia haemolytica serotype 2.previous structural studies in our laboratory on lipopolysaccharide derived core oligosaccharide had identified a conserved inner core structure in several strains of the veterinary pathogens mannheimia haemolytica, actinobacillus pleuropneumoniae and pasteurella multocida. in this study we describe the elucidation of the core oligosaccharide structure of two strains from m. haemolytica serotype 2. structural information was established by a combination of monosaccharide and methylation analyses ...201121601179
capsular serotyping of pasteurella multocida from various animal hosts - a comparison of phenotypic and genotypic methods.one hundred and fourteen strains of pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). the serogroups of p. multocida were determined by both conventional capsular serotyping and a multiplex pcr assay targeting specific capsular genes. based on the conventional serotyping method, the 114 strains of p. multocida were subtyped i ...201121602769
comamonas testosteronan synthase, a bifunctional glycosyltransferase that produces a unique heparosan polysaccharide analog.glycosaminoglycans [gags] are linear hexosamine-containing polysaccharides. these polysaccharides are synthesized by some pathogenic bacteria to form an extracellular coating or capsule. this strategy forms the basis of molecular camouflage since vertebrates possess naturally-occurring gags that are essential for life. a recent sequence database search identified a putative protein from the opportunistic pathogen comamonas testosteroni that exhibits similarity to the pasteurella multocida glycos ...201121610195
multilocus sequence typing of a global collection of pasteurella multocida isolates from cattle and other host species demonstrates niche association.abstract: background: pasteurella multocida causes disease in many host species throughout the world. in bovids, it contributes to bovine respiratory disease (brd) and causes haemorrhagic septicaemia (hs). previous studies have suggested that brd-associated p. multocida isolates are of limited diversity. a multilocus sequence typing (mlst) scheme for p. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method us ...201121612618
enzymatic actions of pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric gtpase g(q).pasteurella multocida toxin (pmt) is a virulence factor responsible for the pathogenesis of some pasteurellosis. pmt exerts its toxic effects through the activation of heterotrimeric gtpase (g(q) , g(12/13) , and g(i) )-dependent pathways, by deamidating a glutamine residue in the α subunit of these gtpases. however, the enzymatic characteristics of pmt are yet to be analyzed in detail, because the deamidation has only been observed in cell-based assays. in this study, we developed rat monoclona ...201121624053
mucosal immunization provides better protection than subcutaneous immunization against pasteurella multocida (b:2) in mice preimmunized with the outer membrane proteins.to investigate the effect of boosting immunity via mucosal route vis-a-vis parenteral route in the mouse model of haemorrhagic septicaemia, mice preimmunized with omp of pasteurella multocida (b:2) were immunized with 10(2) cfu of p. multocida via intranasal and subcutaneous routes. mice were challenged through intranasal route (natural route of infection) with 10(8) cfu 14 days after immunization. group of mice which were immunized intranasally showed significant protection (p < 0.05) of 88% as ...201121633791
pasteurella multocida lipopolysaccharide: the long and the short of it.pasteurella multocida is a capsulated, gram-negative cocco-bacillus that can cause serious disease in a wide range of mammals and birds. p. multocida strains are classified into 16 serovars based on lipopolysaccharide (lps) antigens. lps is an essential virulence factor of p. multocida; mutants expressing severely truncated lps are completely attenuated in chickens. lps is also a major immunogen of p. multocida and protection against infections caused by p. multocida is generally considered to b ...201121664074
a review of hemorrhagic septicemia in cattle and buffalo.hemorrhagic septicemia (hs), an acute, fatal and septicemic disease of cattle and buffaloes caused by pasteurella multocida, is important in tropical regions of the world, especially in african and asian countries. the prevalence of disease has been well documented with predominant isolation of p. multocida serotypes b:2 and e:2. conventional methods of identification such as serotyping, biotyping, antibiogram determination and pathogenicity as well as molecular methods (p. multocida-specific po ...201121676341
kinetics of the response of four positive acute phase proteins in pigs experimentally infected with toxigenic pasteurella multocida.in the present study, acute phase proteins (app) responses in pigs after infection with toxigenic strain of pasteurella multocida (pm) were evaluated. twelve piglets from a herd free from toxigenic pm were used. six of them were infected intranasally with pm. crp, hp, saa and pig-map concentrations were measured using commercial elisa tests. crp and hp were significantly induced from 2 days post inoculation (dpi). the concentration of hp in inoculated pigs remained elevated until the end of the ...201121676559
[epidural abscess caused by pasteurella pneumotropica.] 201121684046
pasteurella multocida septicaemia in a patient on haemodialysis.this case report details the presentation of a 76-year-old man on haemodialysis who rapidly developed overwhelming sepsis over a 24 h period with isolation of the pathogen pasteurella multocida in blood cultures. despite treatment with antibiotics shown to have good activity against this pathogen, the patient died. during further questioning, it transpired that the patient had allowed his dog to lick him, including the neck haemodialysis cannula, though no direct trauma in the form of bites or s ...200921687004
investigating the candidacy of lipopolysaccharide-based glycoconjugates as vaccines to combat mannheimia haemolytica.inner core lipopolysaccharide (lps) has been shown to be conserved in the majority of veterinary strains from the species mannheimia haemolytica, actinobacillus pleuropneumoniae and pasteurella multocida and as such is being considered as a possible vaccine antigen. the proof-in-principle that a lps-based antigen could be considered as a vaccine candidate has been demonstrated from studies with monoclonal antibodies raised to the inner core lps of mannheimia haemolytica, which were shown to be b ...201121701793
combinations of macrolide resistance determinants in field isolates of mannheimia haemolytica and pasteurella multocida.respiratory tract infections in cattle are commonly associated with the bacterial pathogens mannheimia haemolytica and pasteurella multocida. these infections can generally be successfully treated in the field with one of several groups of antibiotics, including macrolides. a few recent isolates of these species exhibit resistance to veterinary macrolides with phenotypes that fall into three distinct classes. the first class has type i macrolide, lincosamide, and streptogramin b antibiotic resis ...201121709086
investigations into an outbreak of corvid respiratory disease associated with pasteurella multocida.the possible cause of disease and mortality in corvids on an outdoor pig unit in the north of england between august 2007 and march 2008 was investigated. nine carrion crows (corvus corone corone) and nine rooks (corvus frugilegus), comprising five live-caught birds with clinical signs of respiratory disease, one live-caught bird without respiratory disease, and 12 birds submitted dead were examined. clinical signs, gross and histopathological examination, microbiology and toxicology indicated t ...201121711193
deep sternal wound infection due to pasteurella multocida: the first case report and review of literature.pasteurella multocida is a gram-negative bacterium recovered from a wide variety of wild and domestic animals and has mostly been associated with infection following animal bites. we present the first reported case of a patient who developed a postoperative sternal wound infection due to p. multocida complicated by bloodstream infection. the outcome was favorable following surgical debridement and antimicrobial therapy. we also review the literature regarding p. multocida postoperative wound inf ...201121713430
relationship of in vitro minimum inhibitory concentrations of tilmicosin against mannheimia haemolytica and pasteurella multocida and in vivo tilmicosin treatment outcome among calves with signs of bovine respiratory disease.objective-to determine associations between in vitro minimum inhibitory concentrations (mics) of tilmicosin against mannheimia haemolytica and pasteurella multocida and in vivo tilmicosin treatment outcome among calves with clinical signs of bovine respiratory disease (brd). design-observational, retrospective, cohort study. animals-976 feeder calves with clinical signs of brd enrolled in 16 randomized clinical trials. procedures-records of clinical trials from october 26, 1996, to november 15, ...201121718206
microbiological identification and analysis of swine tonsils collected from carcasses at slaughter.the primary objective of this 7-month study was to determine the prevalence of porcine pathogens of the tonsil of the soft palate of swine at slaughter. additional objectives were to determine if sampling the carcasses of normal or abnormal hogs provided different microbiological profiles and if the slaughter plant provides a feasible sampling frame and environment for detecting and monitoring important pathogens in tonsils that have health implications for both swine and humans. a total of 395 ...201121731180
expression of matrix metalloproteinase-9 and -12 in porcine lung infections.matrix metalloproteinases (mmps) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. during the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine mmps are limited. in the present study species-specific antibodies were produced to investigate the expression of mmp-9 and mmp-12 immunohistochemically in lungs from pigs infected with ac ...201121737093
inhibition of capsular protein synthesis of pasteurella multocida strain p-1059.a mutant strain pba322 was constructed by electroporation of phagemid containing coding region of antisense rna of the omph gene, encoding 39 kda capsular protein or omph, into parental strain p-1059 (serovar a:3) of pasteurella multocida and determined the pathogenicity in mice and chickens. grayish colonies of the mutant, indicating loss of capsule synthesis, were observed by the obliquely-transmitted light stereomicroscope when compared to iridescent colonies of the parental strain. moreover, ...201121747216
mucoadhesive and ph-sensitive thiolated eudragit microspheres for oral delivery of pasteurella multocida antigens containing dermonecrotoxin.in this study, cysteine was conjugated to the eudragit to have mucoadhesive and ph-sensitive properties. pasteurella multocida dermonecrotoxin (pmt) is a major virulence factor as a causative agent of atrophic rhinitis (ar) in swine and, therefore, inactivated p. multocida was used as a candidate vaccine in the current study. pmt-loaded thiolated eudragit microspheres (tems) prepared using w/o/w emulsion-solvent evaporation method were characterized to assess their efficacy in oral vaccination. ...201121780423
fatty acid intake alters growth and immunity in milk-fed calves.the aim of the present study was to determine the effect of supplementing milk replacer (mr) with neotec4 (provimi north america, brookville, oh), a commercially available blend of butyric acid, coconut oil, and flax oil, on calf growth, efficiency, and indices of immune function. in trial 1a, 48 male holstein calves were fed either a control mr that contained only animal fat or the same mr with neotec4 (treatment) along with free-choice starter. the mr (28.7% crude protein, 15.6% fat) was fed a ...201121787930
evaluation of the biolog system for the identification of certain closely related pasteurella species.the zoonotic impact of pasteurella species in human wounds caused by cats and dogs has increased recently. in this study, the effectiveness of the biolog microstation id system (biolog, hayward, ca) for the identification of certain species of pasteurella sensu stricto was analysed. thirty-eight isolates originating from dogs and cats were studied by pheno- and genotypic methods. the classical biochemical tests identified these isolates as p. multocida, p. dagmatis, and p. canis, while the biolo ...201121795002
comparative analysis of histophilus somni ibpa with other fic enzymes reveals differences in substrate and nucleotide specificities.a new family of adenylyltransferases, defined by the presence of a fic domain, was recently discovered to catalyze the addition of adenosine mono-phosphate (amp) to rho gtpases (yarbrough et al., 2009, science, 323:269; worby et al., 2009, mol. cell, 34:93). this adenylylation event inactivates rho gtpases by preventing them from binding to their downstream effectors. we reported that the fic domain(s) of the protein ibpa from the pathogenic bacterium histophilus somni adenylylates mammalian rho ...201121795713
pasteurella canis peritonitis in a peritoneal dialysis patient. 201121799062
molecular signatures (conserved indels) in protein sequences that are specific for the order pasteurellales and distinguish two of its main clades.the members of the order pasteurellales are currently distinguished primarily on the basis of their branching in the rrna trees and no convincing biochemical or molecular markers are known that distinguish them from all other bacteria. the genome sequences for 20 pasteurellaceae species/strains are now publicly available. we report here detailed analyses of protein sequences from these genomes to identify conserved signature indels (csis) that are specific for either all pasteurellales or its ma ...201121830122
prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves.the prevalence of respiratory pathogens in diseased veal calves was determined in 24 respiratory disease outbreaks in 15 herds in belgium. bacteria were cultured from nasopharyngeal swabs and seroconversion against viruses and mycoplasma bovis was determined on paired sera. at the individual calf level, mycoplasma species, mannheimia haemolytica and pasteurella multocida, were isolated from 70.5 per cent, 21.5 per cent and 26.0 per cent of swabs, respectively. at the herd level, the presence of ...201121831999
classification of pasteurella sp. b as pasteurella oralis sp. nov.pasteurella species b has so far only been reported from the oral cavity of dogs, cats and a ferret. in the present study, information from 15 recent isolates that included african hedgehogs (atelerix albiventris), banded mongoose (mungos mungo), moholi bushbabies (galago moholi) and pneumonia of a cat were compared to five strains investigated previously from bite wounds in humans inflicted by a cat and dog and from gingiva of a cat. rpob gene sequence comparison showed 17 isolates including th ...201121841008
Decreasing the sialidase activity of multifunctional Pasteurella multocida a2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis.Pasteurella multocida a2-3-sialyltransferase 1 (PmST1) is a multifunctional enzyme which has a2-6-sialyltransferase, a2-3-sialidase, and a2-3-trans-sialidase activities in addition to its major a2-3-sialyltransferase activity. The presence of the a2-3-sialidase activity of PmST1 complicates its application in enzymatic synthesis of a2-3-linked sialosides as the product formed can be hydrolyzed by the enzyme. Herein we show that the a2-3-sialidase activity of PmST1 can be significantly decreased ...201121858283
one-pot three-enzyme synthesis of udp-glcnac derivatives.a pasteurella multocida n-acetylglucosamine 1-phosphate uridylyltransferase (pmglmu) was cloned and used efficiently with an n-acetylhexosamine 1-kinase (nahk_atcc55813) and an inorganic pyrophosphatase (pmppa) for one-pot three-enzyme synthesis of udp-glcnac derivatives with or without further chemical diversification.201121863157
[Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut].We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping.201121866710
associations between biovar and virulence factor genes in pasteurella multocida isolates from pigs in spain.two hundred and five isolates of pasteurella multocida from pigs were phenotypically and genetically characterised by determining their biovar, capsular type, virulence-associated genes and pulsed-field gel electrophoresis (pfge) profiles. all isolates were identified as p multocida subspecies multocida and most were assigned to biovar 3 (58 per cent) and biovar 2 (39.5 per cent). biovar 1 represented 2.4 per cent of the isolates. according to the capsular type, the great majority of the isolate ...201121868435
identification and immunogenicity of mannheimia haemolytica s1 outer membrane lipoprotein plpf.immunity against mannheimia haemolytica requires antibodies against leukotoxin (lkt) and bacterial cell surface antigens, most likely immunogenic outer membrane proteins (omps). five immunogenic outer membrane lipoproteins identified and characterized in m. haemolytica were designated pasteurella lipoproteins (plp) a, -b, -c, -d and -e. using immunoproteomics, we identified a heretofore-uncharacterized m. haemolytica immunogenic outer membrane lipoprotein that we designated plpf, which was previ ...201121875637
polymerase chain reaction-based identification of clinically relevant pasteurellaceae isolated from cats and dogs in poland.a set of polymerase chain reaction (pcr) assays for identification of the most important pasteurellaceae species encountered in cats and dogs were developed. primers for pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. primers specific to pasteurella canis, pasteurella dagmatis, and pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (soda) and those specific to [haemophilus] haemoglobinophilus on sp ...201121908285
polymerase chain reaction-restriction fragment length polymorphism analysis of the genes involved in the biosynthesis of the lipopolysaccharide of pasteurella multocida.the lipopolysaccharide, also known as the somatic antigen or o-antigen, is an important virulence factor of pasteurella multocida. in the current study, the genes involved in the biosynthesis of the outer core region of the lipopolysaccharide, which were obtained from somatic type reference strains and field strains of p. multocida, were subjected to polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis. the pcr-rflp analysis classified 11 out of the 16 serotypes ...201121908287
genome sequence of pasteurella multocida subsp. gallicida anand1_poultry.we report the finished and annotated genome sequence of pasteurella multocida gallicida strain anand1_poultry, which was isolated from the liver of a diseased adult female chicken. the strain causes a disease called "fowl cholera," which is a contagious disease in birds. we compared it with the published genome sequence of pasteurella multocida pm70.201121914901
efficacy and safety of a new 450 mg/ml florfenicol formulation administered intramuscularly in the treatment of bacterial bovine respiratory disease.the objective of the study was the safety and efficacy evaluation of a new 450 mg/ml florfenicol formulation in the treatment of naturally occurring respiratory disease when administered intramuscularly, compared with a positive control group treated with the well-established 300 mg/ml formulation. a total of 174 calves, selected from five sites in france and spain, aged from 1 to 17 months, showing severe signs of respiratory disease, were randomly assigned to treatment with either the 300 mg/m ...201121921060
pasteurella multocida zoonotic ascending infection: an unusual cause of tubo-ovarian abscess.abstract we report a tubo-ovarian abscess due to pasteurella multocida. this zoonotic infection was likely of ascending origin, as pasteurella was also isolated from vaginal swabs.201121923258
Pasteurella multocida involved in respiratory disease of wild chimpanzees.Pasteurella multocida can cause a variety of diseases in various species of mammals and birds throughout the world but nothing is known about its importance for wild great apes. In this study we isolated P. multocida from wild living, habituated chimpanzees from Taï National Park, Côte d'Ivoire. Isolates originated from two chimpanzees that died during a respiratory disease outbreak in 2004 as well as from one individual that developed chronic air-sacculitis following this outbreak. Four isolate ...201121931664
opportunistic pathogens relative to physicochemical factors in water storage tanks.household water in oman, as well as in other countries in the region, is stored in tanks placed on house roofs that can be subjected to physicochemical factors which can promote microbial growth, including pathogens and opportunistic pathogens which pose health risks. water samples were collected from 30 houses in a heavily populated suburb of muscat. the tanks used were either glass reinforced plastic (grp), polyethylene or galvanised iron (gi). heterotrophic bacteria, coliforms, faecal colifor ...201121942202
bovine respiratory disease: efficacy of different prophylactic treatments in veal calves and antimicrobial resistance of isolated pasteurellaceae.the present study was conducted to evaluate the efficacy of two prophylactic antibiotic treatments against bovine respiratory disease (brd) in veal calves. in addition, the antibiotic susceptibilities of isolated pasteurellaceae were tested. the calves were treated either on the day of arrival by a single administration of tulathromycin (group a, n=20), by a peroral administration of chlortetracycline, sulphadimidine, and tylosin (group b, n=20) for seven consecutive days, or were not prophylact ...201121945813
Membrane interaction of Pasteurella multocida toxin involves sphingomyelin.Pasteurella multocida toxin (PMT) is an AB toxin that causes pleiotropic effects in targeted host cells. The N-terminus of PMT (PMT-N) is considered to harbor the membrane receptor binding and translocation domains responsible for mediating cellular entry and delivery of the C-terminal catalytic domain into the host cytosol. Previous studies have implicated gangliosides as the host receptors for PMT binding. To gain further insight into the binding interactions involved in PMT binding to cell me ...201121951695
pasteurella multocida cmp-sialic acid synthetase and mutants of neisseria meningitidis cmp-sialic acid synthetase with improved substrate promiscuity.cytidine 5'-monophosphate (cmp)-sialic acid synthetases (csss) catalyze the formation of cmp-sialic acid from ctp and sialic acid, a key step for sialyltransferase-catalyzed biosynthesis of sialic acid-containing oligosaccharides and glycoconjugates. more than 50 different sialic acid forms have been identified in nature. to facilitate the enzymatic synthesis of sialosides with diverse naturally occurring sialic acid forms and their non-natural derivatives, cmp-sialic acid synthetases with promi ...201121968653
the development of a rapid sybr green i-based quantitative pcr for detection of duck circovirus.this report describes a one-step real-time polymerase chain reaction assay based on sybr green i for detection of a broad range of duck circovirus (ducv). align with all ducv complete genome sequences and other genus circovirus download from the genbank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of ducv were designed. the detection assay was linear in the range of 1.31 × 102-1.31 × 107 copies/μl. the reaction efficiency of the assay using the slope ( ...201121978576
ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes.Background In recent years, multiresistant Pasteurella multocida isolates from bovine respiratory tract infections have been identified. These isolates have exhibited resistance to most classes of antimicrobial agents commonly used in veterinary medicine, the genetic basis of which, however, is largely unknown. Methods Genomic DNA of a representative P. multocida isolate was subjected to whole genome sequencing. Genes have been predicted by the YACOP program, compared with the SWISSProt/EMBL da ...201222001175
ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer.Background Integrative and conjugative elements (ICEs) have not been detected in Pasteurella multocida. In this study the multiresistance ICEPmu1 from bovine P. multocida was analysed for its core genes and its ability to conjugatively transfer into strains of the same and different genera. Methods ICEPmu1 was identified during whole genome sequencing. Coding sequences were predicted by bioinformatic tools and manually curated using the annotation software ERGO. Conjugation into P. multocida, M ...201222001176
Characterization of the lipopolysaccharide from Pasteurella multocida Heddleston serovar 9; identification of a proposed bi-functional dTDP-3-acetamido-3,6-dideoxy-{alpha}-D-glucose biosynthesis enzyme.Pasteurella multocida strains are classified into 16 different LPS serovars using the Heddleston serotyping scheme. Ongoing studies in our laboratories on the lipopolysaccharide (LPS) aim to determine the core oligosaccharide (OS) structures expressed by each of the Heddleston type strains and identify the genes and transferases required for the biosynthesis of the serovar-specific oligosaccharides. In this study we have determined the core OS of the LPS expressed by the Heddleston serovar 9 typ ...201122002973
microbial ecology of swine farms and prrs vaccine vaccination strategies.the present study investigated the microbial ecology and vaccination strategies against porcine reproductive and respiratory syndrome (prrs) in field condition. four representative farms with a history of prrs were included in this study. over the almost 3-year period, the average detection rate was 68.9%, making prrsv the first most frequently detected virus, followed by porcine circovirus type 2 (pcv2) (64.2%), pseudorabies virus (prv) (11.03%) and classical swine fever virus (csfv) (4.41%). s ...201122014373
plasmid-mediated florfenicol resistance in mannheimia haemolytica isolated from cattle.the aim of this study was to analyse a florfenicol-resistant mannheimia haemolytica isolated from a calf to determine the genetic basis of its florfenicol-resistance. the antimicrobial susceptibility and plasmid content of the isolate were determined. a florfenicol resistant plasmid carrying the flor gene was identified by pcr and transformed into escherichia coli jm109 and hb101 strains. the plasmid was then mapped and sequenced completely. the isolate was resistant to chloramphenicol, florfeni ...201122019290
Capsular polysaccharide vaccine for Group B Neisseria meningitidis, Escherichia coli K1, and Pasteurella haemolytica A2.We reviewed the literature that is the basis for our proposal that (2?8)-a-Neu5Ac conjugates will be safe and effective vaccines for Group B meningococci (GBMs), Escherichia coli K1, and Pasteurella haemolytica A2. Although (2?8)-a-Neu5Ac is a virulence factor and a protective antigen of these three pathogens, it is also a component of normal tissues (neural cell adhesion molecule). Natural, anti-(2?8)-a-Neu5Ac present in most adults, vaccine-induced antibodies, and even high levels of spontaneo ...201122025709
comparative pharmacokinetics of orbifloxacin in healthy and pasteurella multocida infected ducks.the pharmacokinetic aspects of orbifloxacin were studied in both healthy and naturally diseased ducks after a single intravenous and intramuscular dose of 5 mg kg⁻¹ body weight. the serum concentrations of orbifloxacin following single intravenous and intramuscular injections were higher in diseased than in healthy ducks. the disposition of orbifloxacin after a single intravenous injection was described by a two-compartment open model in both healthy and diseased ducks. orbifloxacin was distribu ...201122029792
pasteurella haemolytica infections in sheep.summary pasteurella haemolytica causes two distinct disease syndromes in sheep. p. haemolytica biotype a causes septicaemia in young lambs and pneumonia in all ages of sheep. biotype t produces an acute systemic disease affecting principally the upper alimentary tract and lungs in young adult sheep. the bacteriology, epidemiology and clinical and necropsy findings of the two diseases are described and the current situation regarding their experimental reproduction and immunology is reviewed.198022039979
preliminary clinical pharmacological investigations of tylosin and tiamulin in chickens.summary the minimal inhibitory concentrations (m1c) of tiamulin and tylosin for mycoplasma. gram-positive, and gram-negative micro-organisms isolated from chickens were determinated by the agar dilution method. median m1c values for tiamulin against mycoplasma gallisepticum (0.05 µg/ml) and mycoplasma synoviae (0.10 µg/ml) were 2 to 4 times lower than the corresponding values for tylosin. tiamulin was also slightly more effective in vitro in inhibiting escherichia coli, pasteurella multocida, ...198022039981
pathogenicity of pasteurella pneumotropica in immunodeficient nod/shijic-scid/jcl and immunocompetent crlj:cd1 (icr) mice.pasteurella pneumotropica is an opportunistic pathogen in rodents. natural infection in immunodeficient animals suggests that immunodeficiency is a major factor in p. pneumotropica pathogenesis. to understand this process, we performed clinical, pathological and bacteriological studies of immunodeficient nod/shijic-scid/jcl and immunocompetent crlj:cd1 (icr) mice experimentally infected with p. pneumotropica atcc 35149. from 14 days postinoculation, some of p. pneumotropica-infected nod/shijic-s ...201122041283
plasma membrane association of three classes of bacterial toxins is mediated by a basic-hydrophobic motif.plasma membrane targeting is essential for the proper function of many bacterial toxins. a conserved fourhelical bundle membrane localization domain (4hbm) was recently identified within three diverse families of toxins: clostridial glucosylating toxins, martx toxins and pasteurella multocida-like toxins. when expressed in tissue culture cells or in yeast, gfp fusions to at least one 4hbm from each toxin family show significant peripheral membrane localization but with differing profiles. both i ...201122044757
Pyogenic cervical spondylitis caused by Pasteurella haemolytica attributed to excessive contact with dogs.The bacterium Pasteurella haemolytica is resident in the oral cavities of dogs and cats and is often a cause of zoonotic infection. However, it is rare for it to be the pathogenic bacteria behind pyogenic spondylitis, and few studies have been conducted on it. This article reports a case of pyogenic cervical spondylitis thought to be caused by excessive contact with pet dogs.A 52-year-old man reported neck pain and pain in the right upper limb. He was admitted after plain radiographs and magneti ...201122049970
arf6-dependent intracellular trafficking of pasteurella multocida toxin and ph-dependent translocation from late endosomes.the potent mitogenic toxin from pasteurella multocida (pmt) is the major virulence factor associated with a number of epizootic and zoonotic diseases caused by infection with this respiratory pathogen. pmt is a glutamine-specific protein deamidase that acts on its intracellular g-protein targets to increase intracellular calcium, cytoskeletal, and mitogenic signaling. pmt enters cells through receptor-mediated endocytosis and then translocates into the cytosol through a ph-dependent process that ...201122053287
Identification of Pasteurella multocida capsular types isolated from rabbits and other domestic animals in Mexico with respiratory diseases.Pasteurella multocida is the causative agent of pasteurellosis, a major disease in most domestic animals and livestock. In this study, a total of 34 isolates of P. multocida from rabbits and other domestic animals from Mexico with respiratory diseases underwent polymerase chain reaction-based capsular typing. One sheep isolate was found to belong to capsular serogroup D, whereas the rest of the rabbit, sheep, cattle, pig, goat, and duck isolates belonged to capsular serogroup A of P. multocida. ...201122068635
pasteurella multocida toxin activates various heterotrimeric g proteins by deamidation.pasteurella multocida produces a 146-kda protein toxin (pasteurella multocida toxin, pmt), which stimulates diverse cellular signal transduction pathways by activating heterotrimeric g proteins. pmt deamidates a conserved glutamine residue of the a-subunit of heterotrimeric g proteins that is essential for gtp-hydrolysis, thereby arresting the g protein in the active state. the toxin substrates are ga(q) ga(13) and the ga(i)-family proteins. activation of these a-subunits causes stimulation of p ...201022069582
pmst3 from pasteurella multocida encoded by pm1174 gene is a monofunctional α2-3-sialyltransferase.pasteurella multocida (pm) strain pm70 has three putative sialyltransferase genes including pm0188, pm0508, and pm1174. a pm0188 gene homolog in pm strain p-1059 encodes a multifunctional α2-3-sialyltransferase, pmst1, that prefers oligosaccharide acceptors. a pm0508 gene homolog in the same strain encodes a monofunctional sialyltransferase pmst2 that prefers glycolipid acceptors. here, we report that the third sialyltransferase from pm (pmst3) encoded by gene pm1174 in strain pm70 is a monofunc ...201122075637
antimicrobial susceptibility patterns and sensitivity to tulathromycin in goat respiratory bacterial isolates.bacterial pneumonia is a common and often life-threatening respiratory problem in both meat and dairy goats. options for approved antibiotic therapy in goats to combat these bacterial infections are severely limited and frequently drugs must be used in an extra-label manner. tulathromycin, a triamilide macrolide antimicrobial drug shown to be effective against swine and cattle respiratory bacterial agents, has been identified as a potentially useful drug in caprines. the present study was conduc ...201122078276
aerobic oral and rectal bacteria of free-ranging steller sea lion pups and juveniles (eumetopias jubatus) in alaska.bacteriologic cultures from oral, rectal, and lesion samples from free-ranging steller sea lion (ssl, eumetopias jubatus) pups and juveniles in alaska (2001-2005) were examined to determine frequency of infection by a specific subset of common and pathogenic aerobic bacteria. associations between isolated bacteria and age, sex, body condition, location, and sampling season were investigated. salmonella spp. isolates were further evaluated to determine spatial clustering (n=48) and to identify se ...201122102651
Balanoposthitis, dyspareunia, and treponema in the critically endangered Gilbert's potoroo (Potorous gilbertii).The Gilbert's potoroo (Potorous gilbertii) is one of Australia's most critically endangered mammals with a current estimated population of 70 individuals. Both the wild and captive populations have a long history of balanoposthitis with associated crusting, ulceration, and preputial discharge. We sought to identify the microbial species found in the discharge, determine their significance in causing balanoposthitis, and correlate these findings with reproductive success and survivorship. Bacteri ...201122102677
time-course investigation of infection with a low virulent pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens.twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (is) or non-immune-suppressed (nis), were challenged with a low virulent strain, pasteurella multocida strain nctc 10322(t), and developed clinical signs and pathological lesions typical of chronic fowl cholera. nis birds demonstrated much more severe signs of fowl cholera than is birds. with few exceptions, signs recorded in is and nis birds were of the same types, but significantly milder in the is birds, indicat ...201122107097
a case of wound dual infection with pasteurella dagmatis and pasteurella canis resulting from a dog bite - limitations of vitek-2 system in exact identification of pasteurella species.background: pasteurella species, widely known as indigenous organisms in the oral and gastrointestinal floras of many wild and domestic animals, are important pathogens in both animals and humans. human infections due to pasteurella species are in most cases associated with infected injuries following animal bites. we encountered a rare case of dual infections caused by different two pasteurella species occurred in a previously healthy 25-year-old female sustaining injury by a dog-bite. - method ...201122112359
Survey of marbofloxacin susceptibility of bacteria isolated from cattle with respiratory disease and mastitis in Europe.A monitoring programme conducted in Europe since 1994 to survey the marbofloxacin susceptibility of bacterial pathogens isolated from cattle has established the susceptibility of bacterial strains isolated before any antibiotic treatment from bovine mastitis and bovine respiratory disease (BRD) cases between 2002 and 2008. Minimum inhibitory concentration (MIC) was determined by a standardised microdilution technique. For respiratory pathogens, Pasteurella multocida and Mannheimia haemolytica is ...201122121154
Pharmacokinetic and pharmacodynamic testing of marbofloxacin administered as a single injection for the treatment of bovine respiratory disease.Vallé, M., Schneider, M., Galland, D., Giboin, H., Woehrlé, F. Pharmacokinetic and pharmacodynamic testing of marbofloxacin administered as a single injection for the treatment of bovine respiratory disease. J. vet. Pharmacol. Therap. doi: 10.1111/j.1365-2885.2011.01350.x. New approaches in Pharmacokinetic/Pharmacodynamic (PK/PD) integration suggested that marbofloxacin, a fluoroquinolone already licensed for the treatment of bovine respiratory disease at a daily dosage of 2 mg/kg for 3-5 days, ...201122126438
Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds.Relationships between macroscopic lesions and Polymerase Chain Reaction (PCR) detection of Mycoplasma hyopneumoniae (Mhp), Pasteurella multocida (Pm), Actinobacillus pleuropneumoniae (App), Haemophilus parasuis (Hps) and Streptococcus suis (Ssuis) of the lungs of 3731 slaughter pigs from 125 herds were assessed in France. Pneumonia and pleuritis were the most frequent lesions (69.3% and 15% of the lungs, respectively). Mhp, Pm, App, Ssuis and Hps were detected in 69.3%, 36.9%, 20.7%, 6.4% and 0. ...201122133708
Polymicrobial respiratory disease in pigs.Respiratory disease in pigs is common in modern pork production worldwide and is often referred to as porcine respiratory disease complex (PRDC). PRDC is polymicrobial in nature, and results from infection with various combinations of primary and secondary respiratory pathogens. As a true multifactorial disease, environmental conditions, population size, management strategies and pig-specific factors such as age and genetics also play critical roles in the outcome of PRDC. While non-infectious f ...201122152290
Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) Analysis of Pasteurella multocida Isolated from Korean Pigs.Pasteurella multocida causes various respiratory disease symptoms in pigs, including atrophic rhinitis and pneumonia. In the present study, 69 strains of P. multocida were isolated from 443 pigs with respiratory clinical symptoms at 182 farms located throughout South Korea from 2009 to 2010. A multiplex capsular PCR typing assay revealed that 69 strains of P. multocida isolated in this study had the biosynthetic locus of the capsules of either serogroup A (47 strains, 68.1%) or serogroup D (22 s ...201122167105
pasteurella multocida pneumonia: zoonotic transmission confirmed by molecular epidemiological analysis. 201222188500
psoas abscess due to pasteurella multocida.psoas abscess is a pus collection within the muscle compartment. it is a very uncommon entity. it can be primary as a result of haematogenous spread or secondary as a consequence of a direct extension of an infectious focus. diagnosis is usually delayed because nonspecific clinical presentation. staphylococcus aureus is the most common organism cultured in this type of abscess. we describe a secondary psoas abscess in an elderly female patient. the patient presented with fever, right groin pain ...201122188964
Sixty Years from the Discovery of Vibrio parahaemolyticus and Some Recollections.Vibrio parahaemolyticus was discovered by Tsunesaburo Fujino after a shirasu food poisoning outbreak in 1950, but at that time the isolate was named Pasteurella parahaemolytica, not Vibrio. Although the isolate resembled Vibrio, some properties did not correspond with those of Vibrio. For example, the curved cell form of the cell was one of the important taxonomical indicators of the genus, but the isolate was straight in form. After 5 years, Iwao Takikawa isolated a similar bacterium from a foo ...201122190435
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