Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
serotypes a1 and a2 of mannheimia haemolytica are susceptible to genotypic, capsular and phenotypic variations in contrast to t3 and t4 serotypes of bibersteinia (pasteurella) trehalosi.mannheimia haemolytica and bibersteinia (pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. the disease is determined by specific serotypes found in cattle and small ruminants. the molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. this study aimed to detect the instability and variations of bacterial strains that may affect the analysis of epide ...200818179587
susceptibility testing of tulathromycin: interpretative breakpoints and susceptibility of field isolates.in vitro susceptibility tests were conducted on bovine and porcine respiratory pathogens isolated from european countries during 2004-2006 for susceptibility to tulathromycin using the recommended methodologies for broth microdilution. the results were compared with data from a similar survey conducted prior to launch in 1998-2001 to monitor for any shift in susceptibility. the importance of maintaining the ph of the culture media within the range 7.2-7.4 was re-affirmed as a key factor in obtai ...200818187275
application of intact cell-based nfat-beta-lactamase reporter assay for pasteurella multocida toxin-mediated activation of calcium signaling pathway.pasteurella multocida toxin (pmt) stimulates and subsequently uncouples phospholipase c beta1 (plcbeta1) signal transduction through its selective action on the alpha subunit of the gq-protein. here, we describe the application of an nfat-beta-lactamase reporter assay as a functional readout for pmt-induced activation of the gq-protein-coupled plcbeta1-ip(3)-ca(2+) signaling pathway. use of the nfat-beta-lactamase reporter assay with a cell-permeable fluorogenic substrate provides high sensitivi ...200818190943
cardiovascular device infections in dogs: report of 8 cases and review of the literature.infection is an infrequent but major complication of cardiovascular device implantation.200718196736
maternal undernutrition and the ovine acute phase response to vaccination.the acute phase response is the immediate host response to infection, inflammation and trauma and can be monitored by measuring the acute phase proteins (app) such as haptoglobin (hp) or serum amyloid a (saa). the plane of nutrition during pregnancy is known to affect many mechanisms including the neuroendocrine and neuroimmune systems in neonatal animals but effects on the app are unknown. to investigate this phenomenon the serum concentration of hp and saa was initially determined in non-stimu ...200818197966
pasteurella multocida in scavenging family chickens and ducks: carrier status, age susceptibility and transmission between species.pasteurella multocida causes fowl cholera, a highly contagious and severe disease in chickens and water fowls. the disease is not well described in less intensive production systems, including scavenging family poultry production in developing countries. p. multocida was isolated from 25.9% of healthy-looking ducks and 6.2% of chickens from free-range family poultry farms and at slaughter slabs at market. on experimental infection with 1.2 to 2.0 x 10(8) organisms of the p. multocida type strain ...200818202950
microbial contamination of dental unit waterlines in istanbul, turkey.the water used in dental unit waterlines (duwls) acts as a coolant for the high-speed equipment and as an irrigant during dental treatments. there are kind of water tanks. duwls provide a favorable environment for microbial biofilm and multiplation primarily due to the high surface in the tubing and the character of fluid dynamics in narrow, smooth-walled waterlines. biofilms can harbour opportunist pathogens such as legionella sp., pseudomonas sp. several studies have shown that duwls have high ...200818210208
field trial of a pasteurella multocida extract vaccine in rabbits.pasteurella multocida is a bacterial pathogen that can cause significant disease and subsequent effects on research activities involving rabbits. although several vaccines have been tested under laboratory conditions, field trials of vaccines for the control of p. multocida in rabbits are few. we used a potassium thiocyanate extract (pte) produced from p. multocida serotype d:3,12,15 to vaccinate pasteurella-free rabbits at their introduction into a colony having endemic infection with p. multoc ...200818210993
disruption of fak signaling: a side mechanism in cytotoxicity.focal adhesion kinase (fak) is a non-receptor protein tyrosine kinase (ptk) which acts as an early modulator in the integrin signaling cascade. fak phosphorylation and its consequent activation regulate several basic biological cellular functions. on the contrary, dysregulation of fak signaling is implicated in the malignant transformation of cells, as well as in nonmalignant pathological conditions. with respect to cytotoxicity, accumulating data indicate that fak participates in the mechanism ...200818215454
pasteurella multocida and bovine respiratory disease.pasteurella multocida is a pathogenic gram-negative bacterium that has been classified into three subspecies, five capsular serogroups and 16 serotypes. p. multocida serogroup a isolates are bovine nasopharyngeal commensals, bovine pathogens and common isolates from bovine respiratory disease (brd), both enzootic calf pneumonia of young dairy calves and shipping fever of weaned, stressed beef cattle. p. multocida a:3 is the most common serotype isolated from brd, and these isolates have limited ...200718218157
immune evasion by pathogens of bovine respiratory disease complex.bovine respiratory tract disease is a multi-factorial disease complex involving several viruses and bacteria. viruses that play prominent roles in causing the bovine respiratory disease complex include bovine herpesvirus-1, bovine respiratory syncytial virus, bovine viral diarrhea virus and parinfluenza-3 virus. bacteria that play prominent roles in this disease complex are mannheimia haemolytica and mycoplasma bovis. other bacteria that infect the bovine respiratory tract of cattle are histophi ...200718218162
microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis).a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ...200718229860
transmission of pathogenic respiratory bacteria to specific pathogen free pigs at slaughter.the purpose of this study was to evaluate the transmission of pathogenic respiratory bacteria to thirteen 5-month-old specific pathogen free (spf) pigs, during the slaughtering process in a commercial slaughterhouse. before transportation, the spf pigs and the lorry were checked to confirm the absence of pathogenic respiratory bacteria. nine spf pigs (group 1) were in contact in a conventional slaughterhouse with finishing pigs, during 4h before slaughtering. four spf pigs (group 2) were slaught ...200818242013
what is your diagnosis? epidural empyema. 200818251990
quest for the diagnosis. case 1: a neonatal zoonosis. neonatal pasteurella multocida septicaemia. 200818254915
experimental infection studies of pasteurella pneumotropica and v-factor dependent pasteurellaceae for f344-rnu rats.to investigate the pathogenicities of p. pneumotropica (pp) and v-factor dependent pasteurellaceae (vfdp) in immunodeficient rats, experimental infections of f344-rnu rats were performed using 3 strains (atcc 35149, cnp 160 and rpz) of pp and 4 strains (v6, v7, v8 and v9) of vfdp. four animals per experimental group were inoculated twice on day 0 and post-inoculation day (pid) 14 with bacterial suspension intranasally. two animals from each group were sacrificed on pid 60 and 120, and examined. ...200818256519
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation.the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ...200818256966
evaluation of pyelonephritis in culled indoor and outdoor high parity sows.the present trial was conducted in hungary in neighboring large indoor and outdoor pig production units, belonging to the same breeding company. rejected kidneys from 201 (out of 241; 83.4%) outdoor, and 191 (out of 512, 37.3%) indoor high parity sows, with previous history of recidiving postparturient fever and excessive postparturient vulvovaginal discharge were gross pathologically bacteriologically, and histologically evaluated. all rejected kidneys revealed chronic pyelonephritis. in outdoo ...200818265757
phenotypic and genotypic characterisation of pasteurella multocida strains isolated from pigs in hungary.a total of 146 pasteurella multocida strains isolated from swine in hungary in the last 20 years were examined. biochemical characterisation and pcr-based techniques were used to determine species, subspecies, biovar, capsule type and presence of the toxa gene. eighty-seven percent of the isolates belonged to p. multocida ssp. multocida, and 98% of these had biovar 3 or were trehalose- or lactose-fermenting or ornithine decarboxylase negative variants of that. ten percent of the strains were p. ...200718277702
anion secretion evoked by pasteurella multocida toxin across rat colon.stimulation of muscarinic receptors is known to have a biphasic effect on colonic cl(-) secretion: a short-lasting activation, which is followed by a long-lasting inhibition. in order to find out, which role gq proteins play in both processes, pasteurella multocida toxin was used, a known activator of g alpha q. this toxin (1.5 microg/ml) had a dual action on short-circuit current (isc) across rat distal colon: it stimulated transiently isc and subsequently down-regulated the isc evoked by ca2+- ...200818279849
promotion of immunity of mice to pasteurella multocida and hog cholera vaccine by pig interleukin-6 gene and cpg motifs.a novel oligodeoxynuleotides containing 11 cpg motifs was synthesized and inserted into the vr1020 plasmid containing pig interleukin-6 (il-6) gene (vpil6) to construct recombinant plasmid, vpil6c. the chitosan nanoparticles (cnp) were prepared by ionic cross linkage to entrap the vpil6c (vpil6c-cnp), vpil6 (vpil6-cnp) and cpg (cpg-cnp). 42-day old female mice were divided into four groups and intramuscularly injected respectively with 6 pmol vpil6c-cnp, vpil6-cnp, cpg-cnp and vr1020-cnp along w ...200918279956
kinetics of growth and leukotoxin production by mannheimia haemolytica in continuous culture.the growth and product formation kinetics of the bovine pathogen mannheimia (pasteurella) haemolytica strain ovi-1 in continuous culture were investigated. the leukotoxin (lkt) concentration and yield on biomass could substantially be enhanced by supplementation of a carbon-limited medium with an amino acid mixture or a mixture of cysteine and glutamine. acetic acid was a major product, increasing to 1.66 g l(-1) in carbon-limited chemostat culture at intermediate dilution rates and accounting f ...200818283506
terpenoid compositions, and antioxidant and antimicrobial properties of the rhizome essential oils of different hedychium species.a phytochemical study of the rhizome essential oils of four different hedychium species was performed by means of gc and gc/ms analyses. h. ellipticum mainly contained 1,8-cineole, sabinene, and terpin-4-ol, while h. aurantiacum possessed terpin-4-ol, para-cymene, and bornyl acetate as the major entities. similarly, trans-meta-mentha-2,8-diene and linalool were noticed in h. coronarium. three different collections (i-iii) of h. spicatum showed amazing differences in the relative contents of thei ...200818293443
[acclimating, screening of dominant bacteria for degrading phthalic acid esters and study on its degradation].to define the method of acclimating isolating and screening of dominant bacteria for phthalic acid esters biodegradation and study their plasmids.200718303624
structural analysis of sialyltransferase pm0188 from pasteurella multocida complexed with donor analogue and acceptor sugar.pm0188 is a newly identified sialyltransferase from p. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (cmp-neuac) to an acceptor sugar. although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. here, we report the x-ray crystal structures of pm0188 in the presence of an acceptor sugar and a donor sugar analogue, rev ...200818304450
associations between pathogens in healthy pigs and pigs with pneumonia.the aim of this study was to evaluate the associations between different pathogens in the development of pneumonia and bronchopneumonia in pigs. samples of bronchoalveolar lavage fluid from 100 pigs showing no clinical signs and 239 pigs with clinical signs of respiratory disease were examined for mycoplasma hyopneumoniae, mycoplasma hyorhinis, us-type porcine respiratory and reproductive syndrome virus (prrsv), eu-type prrsv, porcine circovirus type 2 (pcv-2), influenza virus type a, alpha-haem ...200818310558
genomic and functional analysis of icepdaspa1, a fish-pathogen-derived sxt-related integrating conjugative element that can mobilize a virulence plasmid.integrating conjugative elements (ices) are self-transmissible mobile elements that transfer between bacteria via conjugation and integrate into the host chromosome. sxt and related ices became prevalent in asian vibrio cholerae populations in the 1990s and play an important role in the dissemination of antibiotic resistance genes in v. cholerae. here, we carried out genomic and functional analyses of icepdaspa1, an sxt-related ice derived from a spanish isolate of photobacterium damselae subsp. ...200818326579
respiratory disease in lambs. 200818326850
evaluation of different api systems for identification of porcine pasteurella multocida isolates.an exhaustive biochemical characterisation of 60 porcine pasteurella multocida clinical isolates recovered from lesions indicative of pneumonia, previously confirmed by pcr and all belonging to the capsular serogroup a, was performed by means of four commercial systems. the api 20ne correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the api 20e method. the high diversity exhibited by the api 50chb/e system, with six different patterns, does not advi ...200818343468
pasteurella multocida septic shock after a cat scratch in an elderly otherwise healthy woman: a case report.pasteurella multocida, a gram-negative coccobacillus, is a commensal in the nasopharynx of many animals. p. multocida infections most commonly involve the skin, soft tissues, and respiratory tract, particularly in immunosuppressed patients. the present case illustrates a severe articular infection caused by this bacterium, leading to septic shock, in an elderly, otherwise healthy woman, after a simple scratch of a cat.200818358966
[pathology of inoculation]. 200818361288
the c3 domain of pasteurella multocida toxin is the minimal domain responsible for activation of gq-dependent calcium and mitogenic signaling.the large 1285-amino-acid protein toxin from pasteurella multocida (pmt) is a multifunctional single-chain polypeptide that binds to and enters eukaryotic cells and acts intracellularly to promote g(q) and g(12/13) protein-dependent calcium and mitogenic signal transduction. previous studies indicated that the intracellular activity domain responsible for pmt action was located within the c-terminal 600-700 amino acids. in this study, we have exogenously expressed a series of n- and c-terminal p ...200818369188
protein kinase d links gq-coupled receptors to camp response element-binding protein (creb)-ser133 phosphorylation in the heart.many growth regulatory stimuli promote camp response element-binding protein (creb) ser(133) phosphorylation, but the physiologically relevant creb-ser(133) kinase(s) in the heart remains uncertain. this study identifies a novel role for protein kinase d (pkd) as an in vivo cardiac creb-ser(133) kinase. we show that thrombin activates a pkcdelta-pkd pathway leading to creb-ser(133) phosphorylation in cardiomyocytes and cardiac fibroblasts. alpha(1)-adrenergic receptors also activate a pkcdelta-p ...200818378685
co-infection of respiratory bacterium with severe acute respiratory syndrome coronavirus induces an exacerbated pneumonia in mice.sars-cov grows in a variety of tissues that express its receptor, although the mechanism for high replication in the lungs and severe respiratory illness is not well understood. we recently showed that elastase enhances sars-cov infection in cultured cells, which suggests that sars development may be due to elastase-mediated, enhanced sars-cov infection in the lungs. to explore this possibility, we examined whether co-infection of mice with sars-cov and pp, a low-pathogenic bacterium which elici ...200818380809
atrophic rhinitis vaccine composition triggers different serological profiles that do not correlate with protection.atrophic rhinitis (ar) is a widespread and economically important disease of swine caused by bordetella bronchiseptica and pasteurella multocida. it can be controlled by vaccination. this study investigates the effect of altering the composition (adjuvants and/or addition of formalin-inactivated p. multocida toxin, fpmt) of conventional vaccines on the serological profile and on protection against ar in swine. a significantly higher b. bronchiseptica specific antibody titre was detected for vacc ...200818401954
protection of chickens from fowl cholera by vaccination with recombinant adhesive protein of pasteurella multocida.the recombinant adhesive protein (rcp39) of pasteurella multocida strain p-1059 (serovar a:3) was prepared and purified with a hybrid condition of affinity chromatography. the rcp39 was highly protective for chickens from fowl cholera by challenge-exposure with parental strain p-1059 or heterologous strain x-73 (serovar a:1) compared to various kind of vaccines. sixteen groups of ten chickens each were subcutaneously inoculated twice with 100, 200 or 400 microg proteins of rcp39, native cp39, na ...200818403068
magnetic resonance imaging features of spinal epidural empyema in five dogs.spinal epidural empyema is defined an accumulation of purulent material in the epidural space of the vertebral canal. spinal epidural empyema should be considered as a differential diagnosis in dogs with pyrexia, spinal pain, and rapidly progressing myelopathy. magnetic resonance (mr) imaging is the imaging test of choice in humans. here, we describe the mr imaging features of five dogs with confirmed spinal epidural empyema. the epidural lesions appeared as high or mixed signal masses in t2-wei ...200818418993
upgrading mouse health and welfare: direct benefits of a large-scale rederivation programme.we report the outcome of a 30-month programme to rederive 310 specific pathogen-free mouse strains to populate a new individually ventilated cage barrier facility at the mary lyon centre (mlc), medical research council (mrc) harwell. the mice were rederived in a self-contained quarantine suite and embryo-recipient females were health-screened to assess microbiological status, before moving their offspring into the new facility. the mlc currently houses approximately 49,000 mice in about 9750 cag ...200818435871
using amplified fragment length polymorphism analysis to differentiate isolates of pasteurella multocida serotype 1.avian cholera, an infectious disease caused by the bacterium pasteurella multocida, kills thousands of north american wild waterfowl annually. pasteurella multocida serotype 1 isolates cultured during a laboratory challenge study of mallards (anas platyrhynchos) and collected from wild birds and environmental samples during avian cholera outbreaks were characterized using amplified fragment length polymorphism (aflp) analysis, a whole-genome dna fingerprinting technique. comparison of the aflp p ...200818436655
epidemic pasteurellosis in a bighorn sheep population coinciding with the appearance of a domestic sheep.a pneumonia epidemic reduced bighorn sheep (ovis canadensis) survival and recruitment during 1997-2000 in a population comprised of three interconnected wintering herds (kenosha mountains, sugarloaf mountain, twin eagles) that inhabited the kenosha and tarryall mountain ranges in central colorado, usa. the onset of this epidemic coincided temporally and spatially with the appearance of a single domestic sheep (ovis aires) on the sugarloaf mountain herd's winter range in december 1997. although o ...200818436671
[characteristics and immunogenicity of the n-terminal and c-terminal recombinants of pasteurella multocida toxin].in this study, five fragments of recombinant subunit pasteurella multocida toxin (pmt) were constructed. only pet28a-n1518 and pet28b-c2115 could be expressed efficiently in escherichia coli. the molecular weight of the fusion proteins was 57 kda and 78 kda .western blot confirmed that the two proteins could specifically react with antiserum against pasteurella multocida toxin. no mice died after the intraperitoneal administration of these two proteins with the dose of 200 microg per mouse, but ...200818438004
[celulitis in an 88-year old woman]. 200818457640
identification of pasteurella multocida chaps-soluble outer membrane proteins.fowl cholera continues to be of concern to the poultry industry, especially for turkey growers. this disease costs the turkey industry millions of dollars annually. in order to develop improved live attenuated vaccines or subunit vaccines, the outer-membrane proteins of pasteurella multocida were examined with the use of proteomics. of the 11 proteins total present in an outer-membrane subfraction of p. multocida, four additional proteins were identified, completing the composition of the deterg ...200818459313
effects of subminimum inhibitory concentrations of antibiotics on the pasteurella multocida proteome: a systems approach.to identify key regulators of subminimum inhibitory concentration (sub-mic) antibiotic response in the pasteurella multocida proteome, we applied systems approaches. using 2d-lc-esi-ms(2), we achieved 53% proteome coverage. to study the differential protein expression in response to sub-mic antibiotics in the context of protein interaction networks, we inferred p. multocida pm70 protein interaction network from orthologous proteins. we then overlaid the differential protein expression data onto ...200818464924
pasteurella multocida bacteremia in a patient with ovarian cancer and chemotherapy-induced neutropenia.background:pasteurella multocida is a commensal organism found in the saliva and oropharynx of domestic animals. it causes a variety of human infections ranging from cellulitis to bacteremia and sepsis. the severity of infection is somewhat related to the immunocompetency of the infected host. an immunocompromised host is more likely to suffer a disseminated infection as a result of contact with this organism than an immunocompetent host. this case report and review of the literature are present ...199518472893
effect of centchroman on cellular and humoral immunity.centchroman (ormeloxifene) is a nonsteroidal selective estrogen receptor modulator that is used as once a week oral contraceptive agent. the effect of centchroman on the immune system was evaluated by using different experimental models such as carbon clearance test, cyclophosphamide induced neutropenia, neutrophil adhesion test, effect on serum immunoglobulins, mice lethality test and indirect haemagglutination test. the first three models namely carbon clearance test, cyclophosphamide induced ...200718476393
avian pasteurellosis: taxonomy of the organisms involved and aspects of pathogenesis.the taxonomy of the family pasteurellaceae pohl 1981 appears to be as complex as that of enterobacteriaceae. 16s rrna sequencing indicates that the family should be divided into more than 20 genera. according to phylogenetic investigations, the genus pasteurella sensu stricto includes three subclusters, two of which represent taxa mainly associated with avian hosts. true species of the genera actinobacillus and haemophilus have not been reported from birds. several new taxa, which have been show ...199718483922
isolation and identification of nad-independent bacteria from chickens with symptoms of infectious coryza.since 1990, nad-independent bacteria have been isolated in south africa from poultry showing respiratory manifestations similar to infectious coryza. a total of 126 isolates was examined biochemically and serologically, using polyclonal as well as monoclonal antibodies. forty isolates were identified as ornithobacterium rhinotra-cheale, some of which agglutinated glutaraldehyde-fixed red blood cells. furthermore, fourteen pasteurella avium isolates, five p. volantium and three pasteurella specie ...199718483930
dose response study of enrofloxacin against riemerella anatipestifer septicaemia in muscovy and pekin ducklings.the efficacy of enrofloxacin against riemerella anatipestifer (formerly pasteurella anatipestifer) septicaemia of muscovy and pekin ducklings was assessed in an artificial challenge model which reproduced typical duck riemerellosis with very high mortality. mortality, clinical signs, gross lesions, microbiological clearance, feed intake and body weight gain were the efficacy criteria studied. a pulsing medication in drinking water for 4 h at 25, 50 or 100 ppm enrofloxacin on the first day, follo ...199718483945
phenotypic and genotypic characters of isolates of pasteurella multocida obtained from back-yard poultry and from two outbreaks of avian cholera in avifauna in denmark.two outbreaks of fowl cholera in the avifauna in denmark, affecting primarily eiders but also cormorants, gulls and oyster-catchers were shown to be caused by the same clone of pasteurella multocida ssp. multocida by restriction enzyme analysis (rea) and ribotyping, using the enzymes hpaii and hhai and phenotypic characterization. this observation indicated spread by migratory birds. it was shown that the outbreak clone was closely related to isolates of pasteurella multocida ssp. multocida obta ...199818484015
meningitis and subgaleal, subdural, epidural empyema due to pasteurella multocida.pasteurella is a gram-negative coccobacillus found in 70-90% of oral cavities of cats, and as well, is isolated from the digestive systems of dogs, rats, rabbits, monkeys, and other animals. pasteurella multocida has been known to cause infections in humans, the most familiar being soft tissue infection after animal bites. however, this organism may affect a variety of systems, causing serious disease. pasteurella multocida can cause septic arthritis, osteomyelitis, pneumonia, endocarditis, meni ...201018486411
detection by in-situ hybridization of pasteurella multocida toxin (toxa) gene in the lungs of naturally infected pigs.in-situ hybridization with a non-radioactive digoxigenin-labelled probe was used to detect the pasteurella multocida toxin (pmt) gene in tissue sections of pneumonic lung from pigs naturally infected with toxigenic p. multocida. the morphology of host cells was preserved despite the relatively high temperature used in the incubation procedure. pulmonary abscessation was observed in 13 pigs naturally infected with toxigenic p. multocida type a (three pigs) or d (10 pigs). in these 13 pigs a stron ...200818495148
a compendium of antibiotic-induced transcription profiles reveals broad regulation of pasteurella multocida virulence genes.the transcriptional responses of pasteurella multocida to eight antibiotics with known mode of actions (moas) and one novel antibiotic compound with an unknown moa were collected to create a compendium of transcriptional profiles for moa studies. at minimal inhibitory concentration the three bactericidal compounds enrofloxacin, cefquinome and the novel compound had a minor impact on gene regulation with approximately 1% of the p. multocida genome affected, whilst the bacteriostatic compounds flo ...200818501535
prevalence of actinobacillus pleuropneumoniae, actinobacillus suis, haemophilus parasuis, pasteurella multocida, and streptococcus suis in representative ontario swine herds.tonsillar and nasal swabs were collected from weanling pigs in 50 representative ontario swine herds and tested for the presence of 5 important bacterial upper respiratory tract pathogens. all but 1 herd (2%) tested positive for streptococcus suis by polymerase chain reaction (pcr); 48% of herds were s. suis serovar 2, 1/2 positive. in all but 2 herds there was evidence of haemophilus parasuis infection. in contrast, toxigenic strains of pasteurella multocida were detected by a p. multocida--enz ...200818505187
proliferation and transmission patterns of pasteurella multocida b:2 in goats.this report describes the proliferation and transmission patterns of pasteurella multocida b:2 among stressful goats, created through dexamethasone injections. thirty seven clinically healthy adult goats were divided into three groups consisted of 15 goats in group a, 11 goats in group b and the remaining 11 in group c. at the start of the study, all goats of group a were exposed intranasally to 1.97 x 10(10) cfu/ml of live p multocida b:2. dexamethasone was immediately administered intramuscula ...200818509941
a comparison of 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever/bovine respiratory disease.the aim of this study was to compare 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever (uf)/bovine respiratory disease (brd). at feedlot arrival, 3882 calves were enrolled in the study and randomly allocated to 2 groups, which were housed by group in 12 pens. at the time of allocation, 1 group (mlv3-bt2) received a multivalent, modified-live viral vaccine containing infectious bovine rhinotracheitis virus (ibrv) and types i and ii bovine viral diarr ...200818512457
pasteurella multocida sialic acid aldolase: a promising biocatalyst.sialic acid aldolases or n-acetylneuraminate lyases (nanas) catalyze the reversible aldol cleavage of n-acetylneuraminic acid (neu5ac) to form pyruvate and n-acetyl-d: -mannosamine (mannac). a capillary electrophoresis assay was developed to directly characterize the activities of nanas in both neu5ac cleavage and neu5ac synthesis directions. the assay was used to obtain the ph profile and the kinetic data of a nana cloned from pasteurella multocida p-1059 (pmnana) and a previously reported reco ...200818521592
prevalence of selected infectious organisms and comparison of two anatomic sampling sites in shelter cats with upper respiratory tract disease.in order to describe the isolation rates of potential pathogens and to compare anatomic sampling site suitability, nasal and pharyngeal swabs were taken from cats with acute clinical upper respiratory disease in a humane society. dna of feline herpesvirus-1 was amplified from 51 of 52 cats sampled, mycoplasma species were cultured or detected by pcr in samples from 34 of 42 cats sampled for both culture and pcr, and bordetella bronchiseptica was isolated from three of 59 cats sampled for aerobic ...200818539065
effects of commingling beef calves from different sources and weaning protocols during a forty-two-day receiving period on performance and bovine respiratory disease.the study objective was to determine health and performance of ranch calves from different preconditioning strategies during a 42-d receiving period when commingled with calves of unknown health histories from multiple sources. steer calves from a single source ranch (ranch) were weaned and immediately shipped to a feedlot (wean, initial bw = 247 +/- 29 kg); weaned on the ranch for 45 d before shipping, but did not receive any vaccinations (wean45, initial bw = 231 +/- 26 kg); or weaned, vaccina ...200818567723
activation of galpha (i) and subsequent uncoupling of receptor-galpha(i) signaling by pasteurella multocida toxin.bacterial protein toxins are powerful tools for elucidating signaling mechanisms in eukaryotic cells. a number of bacterial protein toxins, e.g. cholera toxin, pertussis toxin (ptx), or pasteurella multocida toxin (pmt), target heterotrimeric g proteins and have been used to stimulate or block specific signaling pathways or to demonstrate the contribution of their target proteins in cellular effects. pmt is a major virulence factor of p. multocida causing pasteurellosis in man and animals and is ...200818583341
respiratory pasteurellosis: infection or colonization?respiratory infection by pasteurella multocida is uncommon and usually occurs in patients with an underlying lung disease, advanced age and/or immunodepression. in this study, we describe the clinical and microbiological characteristics of a series of 14 patients in whom pasteurella multocida was isolated from lower respiratory tract samples, over a period of 21 y (1986-2006). differentiation between colonization and infection allows establishment of early antibiotic treatment.200818584547
what is your diagnosis? respiratory abscess. 200818593309
effect of age at the time of vaccination on antibody titers and feedlot performance in beef calves.to compare antibody responses, feedlot morbidity and mortality rates, feedlot performance, and carcass value for calves vaccinated with 1 of 2 vaccination strategies and for unvaccinated control calves.200818593324
vaccination with pasteurella multocida recombinant ompa induces strong but non-protective and deleterious th2-type immune response in mice.pasteurella multocida ompa (pmompa) belongs to the major and multifunctional escherichia coli ompa family of proteins. we have previously reported that the protein is conserved, immunogenic and an adhesin that binds host cells and host cell extracellular matrix molecules [dabo sm, confer aw, quijano-blas ra. molecular and immunological characterization of pasteurella multocida serotype a:3 ompa: evidence of its role in p. multocida interaction with extracellular matrix molecules. microb pathog 2 ...200818598730
prevalence of antimicrobial resistance among bacterial pathogens isolated from cattle in different european countries: 2002-2004.the project "antibiotic resistance in bacteria of animal origin - ii" (arbao-ii) was funded by the european union (fair5-qlk2-2002-01146) for the period 2003-2005, with the aim to establish a continuous monitoring of antimicrobial susceptibility among veterinary laboratories in european countries based on validated and harmonised methodologies. available summary data of the susceptibility testing of the bacterial pathogens from the different laboratories were collected.200818611246
usage of antimicrobials and occurrence of antimicrobial resistance among bacteria from mink.the usage of antimicrobials for treatment of mink and the occurrence of antimicrobial resistance among the most important bacterial pathogens in mink was investigated. the aim of the study was to provide data, which may serve as a basis for the formulation of recommendations for prudent use of antimicrobials for mink. a total of 164 haemolytic staphylococci, 49 haemolytic streptococci, 39 pseudomonas aeruginosa, 13 pasteurella multocida, and 1093 escherichia coli isolates from danish mink were i ...200918620819
membrane morphology and leukotoxin secretion are associated with a novel membrane protein of aggregatibacter actinomycetemcomitans.gram-negative bacteria display either a flat or an irregular outer membrane. the periodontal pathogen aggregatibacter (actinobacillus) actinomycetemcomitans has an irregular outer membrane. we have identified a gene that is associated with the biogenesis of this morphology. the gene is part of a three-gene operon and codes for a 141-kda protein designated morphogenesis protein c (morc), which is conserved in several gram-negative bacteria including haemophilus influenzae and pasteurella multocid ...200818621903
salpingitis in web-footed birds: prevalence, aetiology and significance.among 4,893,447 ducks slaughtered during a 10 year period in a single slaughterhouse in denmark 7,190 (0.15%) were condemned because of salpingitis. a further 21,658 ducks (0.44%) were approved for human consumption after the inflamed salpinx had been removed. annual condemnation due to salpingitis varied from 0 to 0.53% and total condemnation varied from 1.30 to 2.72%, with an average of 1.98%. sixteen different bacterial species were isolated in pure culture from salpingitis in 7 week-old duck ...199518645801
nad (v-factor)-independent and typical haemophilus paragallinarum infection in commercial chickens: a five year field study.an unusual bacterium causing respiratory disease in chickens emerged in south africa in february 1989. the disease resembled infectious coryza but the organism differed from typical haemophilus paragallinarum especially in that it did not require v-factor for growth. it has been termed an nad-independent h. paragallinarum. a study of avian haemophili isolated from diseased chickens in kwazulu-natal over the past five years revealed the presence of typical h. paragallinarum, nad-independent h. pa ...199518645802
molecular phylogenetic analysis of tryptophanyl-trna synthetase of actinobacillus actinomycetemcomitans.aminoacyl-trna synthetase family enzymes are of particular interest for creating universal phylogenetic trees and understanding the gene flow as these enzymes perform the basic and analogous biochemical function of protein synthesis in all extant organisms. among them, tryptophanyl-trna synthetase (trp-rs) plays a foremost role in phylogeny owing to the close relationship with tyrosine-trna synthetase. in this study, the sequence of the gene trp-rs was amplified using degenerated adenylation dom ...200818669030
serotypes of 'pasteurella' anatipestifer isolates from ducks in singapore: a proposal of new serotypes.'pasteurella' anatipestifer (pa) isolates from local ducks were typed by slide and tube agglutination tests using antisera against representative strains of existing serotypes. as the strains of serotype 13 and serotype 17 were found to be serologically identical, it is proposed that they be jointly designated as serotype 13. it is also proposed that the english serotype p, represented by strain hprs 2565, be adopted as a replacement for the existing serotype 4 which has been excluded as pa. thr ...199218670960
neurological bacterial diseases in possums and gliders: a review of eight cases. 200818673470
mannheimia haemolytica chimeric protein vaccine composed of the major surface-exposed epitope of outer membrane lipoprotein plpe and the neutralizing epitope of leukotoxin.mannheimia haemolytica is commonly identified in cattle with shipping fever pneumonia. vaccines currently available do not provide complete protection against the disease. in an effort to develop a vaccine that delivers the immunogenic regions of leukotoxin (lkt) a and the outer membrane protein (omp) plpe, a total of four chimeric proteins were constructed. mice were subcutaneously immunized with 25, 50 and 75 microg quantities of each chimeric protein. the specificity of the immune response wa ...200818675308
virulence and infectivity of a: 14 strains of pasteurella multocida for turkey poults.strains of pasteurella multocida, capsular serogroup a and somatic serotype 14, (a: 14) isolated from turkeys which had succumbed to fowl cholera were examined for virulence and infectivity. groups of 2-week-old poults were injected intramuscularly with one of three strains. slight to moderate virulence, based on mortality, was noted. poults that survived exposure were frequently affected with exudative airsacculitis. intramuscular, intra-air sac and intranasal exposure of 6-week-old poults with ...198918679893
vaccination of young turkeys against fowl cholera.turkey poults vaccinated at one day of age with commercial inactivated pasteurella multocida (pm) bacterin responded with low titres of antibody (ab) as measured by elisa, but had an acceptable degree of resistance when challenged with the x-73 strain of pm until 13 weeks of age. turkeys vaccinated at 3 and/or 6 weeks of age responded with higher ab titres and were resistant to virulent challenge with pm. turkey poults vaccinated at 3 and/or 6 weeks of age with a commercial live pm vaccine had a ...199018679920
muscovy ducks as a source of pasteurella anatipestifer infection in turkey flocks.an outbreak of disease, characterised by neurological signs and increased mortality, occurred in 17 out of 50 turkey farms in a village. virological tests were negative as were routine bacteriological tests of the organs. however, pasteurella anatipestifer was repeatedly isolated from the brain. the source of the infection was probably a group of muscovy ducks.199018679922
the pathogenesis of turkey rhinotracheitis virus in turkey poults inoculated with the virus alone or together with two strains of bacteria.turkey rhinotracheitis virus (trtv) was recovered in large amounts from the upper respiratory tract of young turkey poults for about 5 days after experimental eyedrop inoculation with either a virulent or an attenuated strain. thereafter, small amounts of virus were isolated, but only for up to 14 days after inoculation. virulent trtv could be recovered infrequently from the internal organs or the intestinal tract but only if bacteria (bordetella avium and a pasteurella-like organism) were admin ...199118680008
serotypes of 'pasteurella' anatipestifer isolates from poultry in different countries.'pasteurella' anatipestifer (pa) isolates from ducks, turkeys and other birds were typed by agglutination tests using antisera against representative strains of existing serotypes. the majority of the isolates were from the usa, but some were from singapore, england and germany. five new serotypes were identified, three from ducks in the usa and two from singapore. four isolates received from germany did not react with any of the available antisera. the representative strain of serotype 4 (h) wa ...199118680018
immunogenicity and safety of a live pasteurella anatipestifer vaccine in white pekin ducklings: laboratory and field trials.oral or aerosol administration of a live trivalent pasteurella anatipestifer (pa) vaccine (sero-types 1, 2 and 5) gave significant protection against homologous challenges in white pekin ducklings. vaccination of one-day-old ducklings by either method provided protection for at least 42 days of age (last challenge). the vaccine did not cause any untoward signs or mortality in one-day-old ducklings exposed to ten times the recommended dose. the vaccine strains did not revert to virulence on 10 ba ...199118680038
aquaculture and florfenicol resistance in salmonella enterica typhimurium dt104. 200818680675
heterologous protective immunization elicited in mice by pasteurella multocida fur omph.different strategies have been developed to produce vaccines against pasteurella multocida. the approach described herein involves overexpression on the bacterial cell surface of fur-regulated iromps (iron-regulated outer-membrane proteins). accordingly, the ability of fur mutants to promote heterologous protection was examined in a swiss mouse animal model. twofur mutants derived from p. multocida were isolated, one of which was also defective in the omph protein. in mice challenged with virule ...200818683628
monodisperse hyaluronan polymers: synthesis and potential applications.in many cases, the cellular response to hyaluronan (ha) depends on the molecular weight (mw) of the polymer chain. most ha preparations from nature or its derivatives possess wide size distributions called polydisperse. new chemoenzymatic synthesis technology allows the production of very narrow size distribution polymers called monodisperse. the use of stoichiometrically controlled and synchronized polymerization reactions allows an assortment of new ha reagents in the range of 10 kda to 2,500 ...200818691084
pathogenic and opportunistic gram-negative bacteria in soil, leachate and air in san nicolás landfill at aguascalientes, mexico.the occurrence of gram-negative pathogenic and opportunistic species, was studied for two years on air. soil and leachate from the san nicolás landfill. aguascalientes, mexico. for soil and leachate four samplings were done, two during the dry season and two during the rainy season. for soil there were 15 sampling points, the leachate samples were taken on the leachate tank. for air, twelve sampling were done in three points of the landfill. twenty pathogenic and/or opportunistic bacteria were i ...200718693549
development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1.we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ...200918706944
[zoonoses transmitted by mouse and rat maintained as laboratory or pet animals].large numbers of mice (mus spp.) and rats (rattus spp.) are maintained for scientific reasons and as pet animals in germany. while laboratory animals are monitored for pathogenic agents, the hygienic status of pet animals is usually completely unknown. despite great efforts, zoonotic infections were reported even in laboratory settings, e.g. with hantavirus (seoul virus), streptobacillus moniliformis, and trichphyton mentagrophytes. however, in current reports, zoonotic infections were transmitt ...200818712260
bacterial cmp-sialic acid synthetases: production, properties, and applications.sialic acids are abundant nine-carbon sugars expressed terminally on glycoconjugates of eukaryotic cells and are crucial for a variety of cell biological functions such as cell-cell adhesion, intracellular signaling, and in regulation of glycoproteins stability. in bacteria, n-acetylneuraminic acid (neu5ac) polymers are important virulence factors. cytidine 5'-monophosphate (cmp)-n-acetylneuraminic acid synthetase (css; ec 2.7.7.43), the key enzyme that synthesizes cmp-n-acetylneuraminic acid, t ...200818716769
characterization of tolc efflux pump proteins from pasteurella multocida.two tolc homologs, pm0527 and pm1980, were identified for pasteurella multocida. a pm0527 mutant displayed increased susceptibility to a range of chemicals, including rifampin (512-fold) and acridine orange (128-fold). a pm1980 mutant showed increased susceptibility to rifampin, ceftazidime, and vancomycin.200818725450
identification of pasteurella multocida isolates of ruminant origin using polymerase chain reaction and their antibiogram study.a total of 100 isolates of pasteurella multocida from various ruminant species (cattle, buffalo and sheep) belonging to different parts of country were identified using pasteurella multocida-pcr (pm-pcr) and capsular pcr assays. pm-pcr revealed an amplicon of approximately 460 bp in all the isolates tested. as regards capsular pcr, 36 of 38 cattle isolates and 30 of 34 buffalo isolates were found to belong to capsular serogroup b whereas rest of the cattle and buffalo isolates belonged to serogr ...200918759064
a new facultatively anaerobic gram-negative fermentative rod obtained from different pathological lesions in poultry and tentatively designated taxon 14.the taxonomic relationship of 33 unclassified avian pasteurella-\]ke organisms obtained from sinusitis in ducks, pharyngitis in turkeys and rhinitis in a pigeon was investigated by phenotypic and genotypic characterisation. the strains investigated formed a homogeneous group, tentatively designated taxon 14. two biovars were demonstrated within taxon 14. both phenotypic and genotypic characters of taxon 14 suggest classification of taxon 14 outside the family pasteurellaceae pohl 1981. the final ...198618766510
acute septicaemic pasteurella multocida infection in an african grey parrot (psittacus erithacus timneh) after a bite from a pet rat (rattus norvegicus).p. multocida infections in man and birds as a sequel to animal bites are reviewed and a case report is presented of a fatal p. multocida septicaemia in a parrot after a bite from a pet rat. the need for immediate intravenous or intramuscular treatment of birds with a penicillin after bites from all mammals is stressed.198618766580
development of an enzyme-linked immunosorbent assay for the detection of humoral antibody to pasteurella anatipestifer.an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to pasteurella anatipestifer in duck sera is described. as part of the initial assay development, micro-titration plates from different manufacturers were assayed for their suitability to bind p. anatipestifer antigen. the nunc immunopiate ii was chosen, on account of its overall reproducibility (5.5% coefficient of variation) and the absence of an edge effect. optimum concentrations of reagents were determined and the ...198718766597
three cases of infection by haemophilus-like bacteria in psittacines.the clinical manifestations of three cases of infection with nad (v-factor)-requiring haemophilus-like bacteria in psittacines are described. case 1 involved members of the platycercus family and produced mainly respiratory signs. in case 2 only conjunctivitis was seen among cockatiels. the third case was a hand-reared african grey parrot which developed a peracute septicaemia. the haemophilus-like bacteria isolated from the three cases differed from each other as well as from the known avian na ...198818766731
taxonomy of some recently described avian pasteurella/actinobacillus-like organisms as indicated by deoxyribonucleic acid relatedness.members of the family pasteurellaceae pohl 1981 are frequently encountered in birds as parasites or pathogens, e.g. the well-known species pasteurella multocida, pasteurella gallinarum, haemophilus paragallinarum, and three species containing strains that had been previously classified as "haemophilus avium"(pasteurella avium, pasteurella volantium, and an unnamed pasteurella species defined by mutters et al., 1985). a variety of additional taxa which had been tentatively assigned to the family ...198518766923
antigenic studies on pasteurella anatipestifer, species incertae sedis, using slide and tube agglutination.comparative serological investigations including all available serotypes of pasteurella anatipestifer, species incertae sedis, have shown that six american serotypes designated by arabic numbers were covered already by previously described serotypes. two out of 12 existing english serotypes designated alphabetically were shown to be identical. it is suggested that arabic numbers are used instead of letters for sero-type designation. consequently, two new serotypes isolated in denmark are designa ...198218770200
reticuloendotheliosis antigen for the agar gel precipitation test.an antigen for gel precipitation was prepared from chick embryo fibroblast cultures inoculated with the reticuloendotheliosis virus. the specificity of the reaction was confirmed with reference reticuloendotheliosis and spleen necrosis sera. no precipitation reactions occurred between this antigen and marek's disease, newcastle disease, infectious bronchitis, pasteurella multocida and haemorrhagic enteritis antisera.197718770334
incidence of pasteurella haemolytica in the respiratory tract of apparently healthy chickens and chickens with infectious bronchitis. characterisation of 213 strains.the tracheal flora of healthy chickens consisted mainly of gram-positive microorganisms. a shift to a predominantly gram-negative flora, mainly due to escherichia, was observed in chickens with respiratory symptoms caused by infectious bronchitis. most of the chickens in which e. coli formed a predominant part of the tracheal flora also showed a positive serological reaction to mycoplasma gallisepticum. a raised incidence of pasteurella haemolytica with increase in age was noted both with regard ...197718770338
studies on pasteurella gallinarum.in two outbreaks of disease in chickens associated with pasteurella gallinarum infection swollen and inflamed wattles were symptoms. a bacteriological study was carried out of 42 p. gallinarum strains isolated from the two outbreaks and from sporadic cases in fowls. serotyping by the gel diffusion precipitin test indicated that all five strains from one outbreak and 82% of isolants from 22 sporadic cases belonged to one serotype, antigenically unrelated to pasteurella multocida. all four isolant ...197718770350
an avirulent live pasteurella multocida vaccine for drinking water and aerosol administration against turkey cholera.a naturally avirulent isolant of pasteurella multocida (heddleston sero-type 3) was investigated as a seed strain for the development of a live vaccine against turkey pasteurellosis. laboratory trials showed that when given in aerosol form at a dosage of 5 x 10(8) c.f.u. per m(2), of barn floor, the vaccine produced satisfactory protection against both homologous and heterologous serotypes challenge administered by aerosol. likewise, turkeys vaccinated once, twice or three times, and challenged ...197918770466
molecular analysis of florfenicol-resistant pasteurella multocida isolates in germany.three florfenicol-resistant pasteurella multocida isolates from germany, two from swine and one from a calf, were investigated for the genetics and transferability of florfenicol resistance.200818786941
fatal pneumonia epizootic in musk ox (ovibos moschatus) in a period of extraordinary weather conditions.the musk ox is adapted to extreme cold and regarded as vulnerable to the impacts of climate change. population decline is proposed to occur due to changes in forage availability, insect harassment, parasite load, and habitat availability, while the possible role of infectious diseases has not been emphasized. the goal of the present article is to describe an outbreak of fatal pasteurellosis that occurred in the introduced musk ox population of dovrefjell, norway in 2006, causing the death of a l ...200818787923
Displaying items 7101 - 7200 of 7610