| endocarditis in a large district general hospital: a study of the microbiological spectrum between 2000 and 2011. | infective endocarditis is one of three common cardiac infections in the united kingdom, in addition to myocarditis and pericarditis, with a reported incidence of 1.7 to 6.2 cases per 100,000 patient years. infective endocarditis can often have serious consequences and a wide variety of organisms may be the causative pathogen. there are little published data regarding the exact spectrum of organisms that cause endocarditis in the united kingdom and whether organisms such as streptococci still dom ... | 2012 | 23592930 |
| permanent pacemaker-associated actinomycetemcomitans endocarditis: a case report. | aggregatibacter actinomycetemcomitans is a gram-negative bacillus, member of the hacek group of bacteria, and it is a very rare cause of endocarditis. it is also an extremely rare cause of device-associated infection of the heart. we describe the case of a 25 year-old man who presented with pacemaker-associated endocarditis due to aggregatibacter actinomycetemcomitans and also discuss the implications and treatment of this organism. | 2013 | 24432293 |
| in vitro efficacy of diallyl sulfides against the periodontopathogen aggregatibacter actinomycetemcomitans. | the in vitro antibacterial effects of diallyl sulfide (das) against the gram-negative periodontopathogen aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. a. actinomycetemcomitans was treated with garlic extract, allicin, or das, and the anti-a. actinomycetemcomitans effects of the treatment were evaluated. garlic extract, allicin, and das significantly inhibited the growth of a. act ... | 2012 | 22330917 |
| lipopolysaccharides mediate leukotoxin secretion in aggregatibacter actinomycetemcomitans. | we previously reported that lipopolysaccharide (lps) -related sugars are associated with the glycosylation of the collagen adhesin emaa, a virulence determinant of aggregatibacter actinomycetemcomitans. in this study, the role of lps in the secretion of other virulence factors was investigated. the secretion of the epithelial adhesin aae, the immunoglobulin fc receptor omp34 and leukotoxin were examined in a mutant strain with inactivated tdp-4-keto-6-deoxy-d-glucose 3,5-epimerase (rmlc), which ... | 2011 | 22394466 |
| platelet-activating factor receptor blockade ameliorates aggregatibacter actinomycetemcomitans-induced periodontal disease in mice. | periodontal disease (pd) is a chronic inflammatory and alveolar bone destructive disease triggered by oral biofilm-producing microorganisms, such as aggregatibacter actinomycetemcomitans. the levels of the phospholipid platelet-activating factor (paf) in the saliva, gingival crevicular fluid, and periodontal tissues are significantly increased during inflammatory conditions, such as pd, but the exact mechanism that links paf to alveolar bone resorption is not well understood. in the current stud ... | 2013 | 24002061 |
| 16s rdna-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease. | acute exacerbations of chronic obstructive pulmonary disease (ae-copd) are leading causes of mortality in hospital intensive care units. we sought to determine whether dental plaque biofilms might harbor pathogenic bacteria that can eventually cause lung infections in patients with severe ae-copd. | 2014 | 24484531 |
| functional contributions of positive charges in the pore-lining helix 3 of the bordetella pertussis cyaa-hemolysin to hemolytic activity and ion-channel opening. | the bordetella pertussis cyaa-hemolysin (cyaa-hly) domain was previously demonstrated to be an important determinant for hemolysis against target erythrocytes and ion-channel formation in planar lipid bilayers (plbs). here, net-charge variations in the pore-lining helix of thirteen related rtx cytolysins including cyaa-hly were revealed by amino acid sequence alignments, reflecting their different degrees of hemolytic activity. to analyze possible functional effects of net-charge alterations on ... | 2017 | 28300777 |
| surface display of aggregatibacter actinomycetemcomitans autotransporter aae and dispersin b hybrid act as antibiofilm agents. | among the various proteins expressed by the periodontopathogen aggregatibacter actinomycetemcomitans, two proteins play important roles for survival in the oral cavity. the autotransporter aae facilitates the attachment of the pathogen to oral epithelial cells, which act as a reservoir, while the biofilm-degrading glycoside hydrolase dispersin b facilitates the movement of daughter cells from the mature biofilm to a new site. the objective of this study was to use the potential of these two prot ... | 2016 | 26280561 |
| the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study. | this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ... | 2016 | 26949701 |
| oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients. | the objective of this study was to identify the dna of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight (pb/lbw) neonates. | 2015 | 26259070 |
| antimicrobial constituents of artemisia afra jacq. ex willd. against periodontal pathogens. | the phytochemical investigation of an ethanol extract of artemisia afra led to the isolation of six known compounds, acacetin (1), 12α,4α-dihydroxybishopsolicepolide (2), scopoletin (3), α-amyrin (4), phytol (5), and a pentacyclic triterpenoid betulinic acid (6). the compounds were evaluated for antimicrobial activity against gram positive (actinomyces naeslundii, actinomyces israelii, and streptococcus mutans), gram negative bacteria (prevotella intermedia, porphyromonas gingivalis and aggregat ... | 2012 | 22693528 |
| the evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection. | secondary/persistent endodontic infection can be the outcome of failure of endodontic treatment. photo-activated disinfection (pad) can be a useful adjunct to mechanical and antimicrobial agents in eliminating endopathogenic microorganisms. in this study, we evaluated the effect of pad on diversity and count of microbiota related to secondary/persistent endodontic infections. | 2017 | 28249746 |
| the use of bacteriophages to biocontrol oral biofilms. | infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ... | 2017 | 28108235 |
| effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria. | to investigate the effect of the roselle calyx extract (rce) (hibiscus sabdariffa l.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria. | 2016 | 26919939 |
| antimicrobial activity of protamine against oral microorganisms. | protamine is an arginine-rich polycationic protein extracted from sperm cells of vertebrates including fishes such as salmon. the purpose of this study was to investigate the suppressive effects of protamine on the growth of oral pathogens for possible usage in dental materials. minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined by the microdilution method. twelve strains of oral viridans streptococci, actinomyces naeslundii, actinomyces odontolyt ... | 2015 | 26699859 |
| periodontal therapy effects on nitrite related to oral bacteria: a 6-month randomized clinical trial. | nitrite is a biologic factor relevant to oral and systemic homeostasis. through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (chx) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. this 6-month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria. | 2015 | 25811847 |
| [antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria]. | this study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. methods in this study, we investigated the antimicrobial activity of decapeptide against streptococcus mutans (s. mutans), streptococcus sobrinus, lactobacillus acidophilus, streptococcus sanguis, streptococcus gordonii, actinomyces viscosus, actinomyces naeslundii, porphyromonas gingivalis, prevotella intermedia, fusob ... | 2014 | 25665430 |
| an in vitro biofilm model associated to dental implants: structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces. | the impact of implant surfaces in dental biofilm development is presently unknown. the aim of this investigation was to assess in vitro the development of a complex biofilm model on titanium and zirconium implant surfaces, and to compare it with the same biofilm formed on hydroxyapatite surface. | 2014 | 25110288 |
| improving photodynamic inactivation of bacteria in dentistry: highly effective and fast killing of oral key pathogens with novel tooth-colored type-ii photosensitizers. | increasing antibiotic resistances in microorganisms create serious problems in public health. this demands alternative approaches for killing pathogens to supplement standard treatment methods. photodynamic inactivation of bacteria (pib) uses light activated photosensitizers (ps) to generate reactive oxygen species immediately upon illumination, inducing lethal phototoxicity. positively charged phenalen-1-one derivatives are a new generation of ps for light-mediated killing of pathogens with out ... | 2014 | 24884918 |
| dental caries and microbiota in children with black stain and non-discoloured dental plaque. | we aimed to assess caries experience and microbiota in systemically healthy children with black stain (bs) and non-discoloured plaque. | 2014 | 24335143 |
| characterization and application of a flow system for in vitro multispecies oral biofilm formation. | bacteria in the oral cavity grow in the form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. the aims of this study were: (i) to develop and to characterize an in-vitro biofilm model with oral bacteria growing under flow and shear conditions; and (ii) to demonstrate the usefulness of the model for evaluating the activity of three antiplaque agents. | 2014 | 23815431 |
| effect of metalloporphyrins on red autofluorescence from oral bacteria. | the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ... | 2013 | 23659237 |
| arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota. | rheumatoid arthritis (ra) and periodontitis (pd) are chronic inflammatory disorders that cause bone loss. pd tends to be more prevalent and severe in ra patients. previous experimental studies demonstrated that ra triggers alveolar bone loss similarly to pd. the aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. checkerboard dna-dna hybridization was employed to analyze forty oral bacterial species in 3 groups of c ... | 2016 | 26996070 |
| influence of complement on neutrophil extracellular trap release induced by bacteria. | neutrophil extracellular trap (net) release has generally been studied in the absence of serum, or at low concentrations of untreated or heat-inactivated serum. the influence of serum complement on net release therefore remains unclear. we examined the dna release induced by staphylococcus aureus and three oral bacteria: actinomyces viscosus, aggregatibacter actinomycetemcomitans and fusobacterium nucleatum subsp. vincettii. | 2016 | 25900429 |
| microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. | the detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. the collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. however, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. the aim of this cross-sectional s ... | 2014 | 24144271 |
| evaluation of the bactericidal activity on oral organisms of the nd-yap laser in vitro. | the bactericidal activity of a pulsed fibre-optic nd-yap laser on various strains of oral bacteria: prevotella nigrescens, streptococcus sanguis, actinomyces viscosus (i), fusobacterium nucleatum, actinobacillus actinomycetemcomitans and peptostreptococcus micros (i) was evaluated. a 200-μm optical fibre was placed in eppendorf tubes containing the different bacterial suspensions. at an energy of 170 mj/30 hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). the expo ... | 1998 | 24710990 |
| survival of an aggregatibacter actinomycetemcomitans quorum sensing luxs mutant in the mouths of rhesus monkeys: insights into ecological adaptation. | experiments were designed to explore a prominent autoinducer-2 (ai-2) producing gene (luxs) related to colonization and survival of aggregatibacter actinomycetemcomitans, a low abundance member of the indigenous flora, that forms a key component of the dysbiotic flora in localized aggressive periodontitis. the luxs gene was disrupted in a primate strain of a. actinomycetemcomitans prior to implantation into the oral cavity of rhesus monkeys (rh). the colonization efficiency of the luxs mutant (r ... | 2017 | 28383798 |
| generalized aggressive periodontitis: microbiological composition and clinical parameters in non-surgical therapy. | the aim of this study was to determine the variations in periodontal parameters and microbiological composition in periodontal pockets at the baseline and 3 and 6 months post treatmentin patients with generalized aggressive periodontitis(gap) undergoing non surgical periodontal treatment combined with chlorhexidine and systemic antibiotics. medical and dental history was taken from 10 subjects, average age 30.6±2.7 years, diagnosed with gap. a non surgical periodontal treatment combined with 0.1 ... | 2016 | 28383606 |
| comparison of nonsurgical periodontal therapy with oral hygiene instruction alone for chronic periodontitis. | we evaluated changes in clinical variables and microbiological profiles of periodontopathogens among 56 patients with moderate to severe cp who were randomly assigned to oral hygiene instruction (ohi; n = 28) or nonsurgical periodontal treatment (nspt; n = 28). periodontal variables were assessed and subgingival plaque samples were obtained from deep pockets (≥5 mm) at baseline and 3 months after treatment. real-time polymerase chain reaction was used to quantify actinobacillus actinomycetemcomi ... | 2017 | 28367891 |
| association between specific periodontal pathogens, toll-like receptor-4, and nuclear factor-κb expression in placental tissues of pre-eclamptic women with periodontitis. | the aim of the present study was to determine the association between the presence of specific periodontal pathogens, toll-like receptor-4 (tlr-4), and nuclear factor-κb (nf-κb) expression in the placental tissues of pre-eclamptic women. | 2017 | 28349674 |
| compositional differences in multi-species biofilms formed on various orthodontic adhesives. | to investigate the aspects of multi-species biofilm formation on various orthodontic adhesives with different surface characteristics. | 2017 | 28339597 |
| reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes. | the aim was to evaluate the intra-test agreement of pooled samples from the deepest periodontal pocket of each quadrant with a commercially available test kit based on hybridization of 16s rrna. | 2017 | 28325127 |
| tio2 nanorod arrays as a photocatalytic coating enhanced antifungal and antibacterial efficiency of ti substrates. | to investigate the photocatalytic inactivation of fungi and bacteria mediated by tio2 nanorod arrays (tnrs). | 2017 | 28322109 |
| quorum sensing of streptococcus mutans is activated by aggregatibacter actinomycetemcomitans and by the periodontal microbiome. | the oral cavity is inhabited by complex microbial communities forming biofilms that can cause caries and periodontitis. cell-cell communication might play an important role in modulating the physiologies of individual species, but evidence so far is limited. | 2017 | 28320314 |
| subgingival microbiota in caucasian patients with desquamative gingivitis: a cross-sectional study. | the presence of epithelial desquamation, erythema, and erosions on the gingival tissue is usually described in literature as desquamative gingivitis (dg). a wide range of autoimmune/dermatological disorders can manifest as dg, although the two more common are oral lichen planus and mucous membrane pemphigoid. the aim of this study was to investigate the prevalence of 11 periodonto-pathogenic microorganisms in dg cases and to compare it with microbiologic status of individuals affected by plaque- ... | 2017 | 28304209 |
| aggregatibacter actinomycetemcomitans osteomyelitis in a 12 year old boy: case report emphasizing the importance of tissue culture, and review of literature. | aggregatibacter actinomycetemcomitans most commonly causes periodontitis but has been reported to infect heart valves, soft tissue, brain and lungs, and distal bones. osteomyelitis distal to the jaw is rarely described. | 2017 | 28288638 |
| efficacy of natural and allopathic antimicrobial agents incorporated onto guided tissue regeneration membrane against periodontal pathogens: an in vitro study. | periodontal disease is one of the most prevalent afflictions worldwide. it is an infection of the periodontium as a result of subgingival colonization of the specific microbiota, leading to loss of attachment, which requires optimal care for regeneration to its pre-disease state. guided tissue regeneration (gtr) is one of the successful treatment modalities in periodontal regenerative therapy, but is vulnerable to bacterial colonization. the conflict between usage of classical antibiotics and pl ... | 2017 | 28274052 |
| oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile. | the aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (oba-9) and fibroblasts (hgf-1) in a dual-chamber model] infected by aggregatibacter actinomycetemcomitans. the periodontopathogen was applied and allowed to cross the top layer of cells (oba-9) to reach the bottom layer of cells (hgf-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/ml or 20 μg ... | 2017 | 28270109 |
| the mechanism of jurkat cells apoptosis induced by aggregatibacter actinomycetemcomitans cytolethal distending toxin. | cytolethal distending toxin (cdt) which is produced by aggregatibacter actinomycetemcomitans causes apoptosis in lymphocytes. but the specific mechanism is not clear. the aim of our research was to investigate the effect and mechanism during this process. the wild-type cdta, cdtb, cdtc (cdta(w), cdtb(w), cdtc(w)) and mutant cdtb (cdtb(m)) were expressed and purified respectively and the purity of each subunit was examined by bandscan software. and the type i deoxyribonuclease and pi-3,4,5-tripho ... | 2017 | 28247205 |
| response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces. | the aim of this study was to develop in vitro biofilms on sla titanium (ti-sla) and zirconium oxide (zro2) surfaces and to evaluate the effect of antiseptic agents on the number of putative periodontal pathogenic species. | 2017 | 28237092 |
| real-time quantitative reverse transcription-pcr analysis of expression stability of aggregatibacter actinomycetemcomitans fimbria-associated gene in response to photodynamic therapy. | aggregatibacter actinomycetemcomitans is an etiological agent of both chronic and aggressive periodontitis. dissemination of a. actinomycetemcomitans from the oral cavity and initiation of systemic infections has led to new approaches for treatment being needed. in this study, a series of experiments presented investigated the effect of methylene blue (mb)-mediated antimicrobial photodynamic therapy (apdt) on cell viability and expression of fimbria-associated gene (rcpa) in a. actinomycetemcomi ... | 2017 | 28216095 |
| differential human th22-lymphocyte response triggered by aggregatibacter actinomycetemcomitans serotypes. | in aggregatibacter actinomycetemcomitans, different serotypes have been described based on lipopolysaccharide (lps) antigenicity. when t lymphocytes were stimulated with these serotypes, different patterns of t-helper (th)1 and th17-type of immune responses were reported. recently, two new th phenotypes have been described and named th9 and th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. this study aimed to investigate the potential th9 and/or th22 lym ... | 2017 | 28189882 |
| development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials. | since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. implant systems have become more sophisticated and have enormously enhanced patients' quality of life. although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. these infections involve the formation of sessile microbial communities, called biofilms. biofilms possess unique ... | 2017 | 28187188 |
| presence of aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children with congenital heart disease. | the purpose of this study was to analyze the presence of aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children requiring cardiac surgery in istanbul, turkey. | 2017 | 28169262 |
| application of diverse natural polymers in the design of oral gels for the treatment of periodontal diseases. | the objective of this study was to prepare periodontal gels using natural polymers such as badam gum, karaya gum and chitosan. these gels were tested for their physical and biochemical properties and assessed for their antibacterial activity against aggregatibacter actinomycetemcomitans and streptococcus mutans, two pathogens associated with periodontal disease. badam gum, karaya gum and chitosan were used to prepare gels of varying concentrations. moxifloxacin hydrochloride, a known antimicrobi ... | 2017 | 28144851 |
| incorporation of silver nanoparticles on the surface of orthodontic microimplants to achieve antimicrobial properties. | microbial aggregation around dental implants can lead to loss/loosening of the implants. this study was aimed at surface treating titanium microimplants with silver nanoparticles (agnps) to achieve antibacterial properties. | 2017 | 28127534 |
| effect of opposing implant prostheses on periodontal pathogens in dentures: a pilot study. | an understanding of the presence of periodontal pathogens in denture plaque is important for the treatment of patients with edentulism. however, current data are limited and inconclusive. | 2017 | 28126355 |
| effects of periodontal treatment on primary sjȫgren's syndrome symptoms. | the aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (essdai) and subjective (esspri) indexes in patients with primary sjögren's syndrome (pss). twenty-eight female patients were divided into four groups: pss patients with or without chronic periodontitis (scp, sc, respectively), and systemically healthy patients with or without chronic periodonti ... | 2017 | 28099577 |
| enzymatic kinetics of the quinol peroxidase of an aggressive periodontopathic bacterium. | aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (qpo) that catalyzes peroxidase activity using quinol in the respiratory chain. in the present work, we have characterized recombinant qpo purified from the membrane fraction of escherichia coli harboring a plasmid containing qpo gene. irreversible inactivation of qpo by high concentration of h2o2 exhibited pseudo-first order kinetics ... | 2017 | 28082720 |
| bacterial short-chain fatty acid metabolites modulate the inflammatory response against infectious bacteria. | short-chain fatty acids (scfas), predominantly acetic, propionic, and butyric acids, are bacterial metabolites with an important role in the maintenance of homeostasis due to their metabolic and immunomodulatory actions. some evidence suggests that they may also be relevant during infections. therefore, we aimed to investigate the effects of scfas in the effector functions of neutrophils to an opportunistic pathogenic bacterium, aggregatibacter actinomycetemcomitans. using a subcutaneous model t ... | 2017 | 28070968 |
| secretable small rnas via outer membrane vesicles in periodontal pathogens. | micrornas (mirnas) have been shown to be major regulators of eukaryotic gene expression. however, bacterial rnas comparable in size to eukaryotic mirnas (18-22 nucleotides) have received little attention. recently, a novel class of small rnas similar in size to mirnas (mirna-size, small rnas or msrnas) have also been found in several bacteria. like mirnas, msrnas are approximately 15 to 25 nucleotides in length, and their precursors are predicted to form a hairpin loop secondary structure. here, ... | 2017 | 28068479 |
| dataset of the proteome of purified outer membrane vesicles from the human pathogen aggregatibacter actinomycetemcomintans. | the gram-negative bacterium aggregatibacter actinomycetemcomitans is an oral and systemic pathogen, which is linked to aggressive forms of periodontitis and can be associated with endocarditis. the outer membrane vesicles (omvs) of this species contain effector proteins such as cytolethal distending toxin (cdt) and leukotoxin (ltxa), which they can deliver into human host cells. the omvs can also activate innate immunity through nod1- and nod2-active pathogen-associated molecular patterns. this ... | 2017 | 28050585 |
| antibacterial effect of aloe vera gel against oral pathogens: an in-vitro study. | natural herbal remedies have shown promising anti-microbial property and fewer side effects compared to synthetic anti-microbial therapy. aloe vera is a medicinal plant used for management of various infections since ancient times as it has anti-inflammatory, anti-microbial, and immune-boosting properties. | 2016 | 28050502 |
| periodontal surgery involving modified widman flap procedure and connective tissue graft for generalized aggressive periodontitis: a case report. | we report a case of generalized aggressive periodontitis (agp) requiring periodontal treatment including flap surgery and ridge augmentation. the patient was a 39-year-old woman who presented with the chief complaint of pus discharge from tooth #36. no other obvious signs of gingival inflammation were observed. periodontal examination revealed multiple sites with a probing depth of ≥10 mm. radiography showed pro-nounced bone defects in the maxillary incisors and molar region. real-time pcr was u ... | 2016 | 28049974 |
| trends in susceptibility to aggressive periodontal disease. | aggregatibacter actinomycetemcomitans is a gram-negative microbe involved in periodontitis. strains with varying degrees of virulence have been identified, in healthy and periodontally compromised individuals alike. hosts mount differential immune responses to its various serotypes and virulence factors. studies have explored host immune response in terms of antibody titers, leukocyte responses, and specific inflammatory mediators, questioning the ways in which the infectious microorganism survi ... | 2016 | 28008419 |
| enhanced antimicrobial activities of silver-nanoparticle-decorated reduced graphene nanocomposites against oral pathogens. | as a means of capitalizing on the synergistic properties between reduced graphene nanosheets (r-gns) and silver nanoparticles (agnps), an efficient and convenient chemical reduction method was used to prepare silver-nanoparticle-decorated reduced graphene nanocomposites (r-gns/ag). the products were characterized by scanning electron microscopy (sem), transmission electron microscopy (tem), x-ray diffraction (xrd), and raman spectroscopy, which confirmed the loading of well-dispersed silver nano ... | 2017 | 27987652 |
| rt-pcr quantification of periodontal pathogens in crack users and non-users. | to compare counts of aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis and fusobacterium nucleatum between crack users and non-users. | 2017 | 27982490 |
| aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid arthritis. | a bacterial etiology of rheumatoid arthritis (ra) has been suspected since the beginnings of modern germ theory. recent studies implicate mucosal surfaces as sites of disease initiation. the common occurrence of periodontal dysbiosis in ra suggests that oral pathogens may trigger the production of disease-specific autoantibodies and arthritis in susceptible individuals. we used mass spectrometry to define the microbial composition and antigenic repertoire of gingival crevicular fluid in patients ... | 2016 | 27974664 |
| microbial community composition impacts pathogen iron availability during polymicrobial infection. | iron is an essential nutrient for bacterial pathogenesis, but in the host, iron is tightly sequestered, limiting its availability for bacterial growth. although this is an important arm of host immunity, most studies examine how bacteria respond to iron restriction in laboratory rather than host settings, where the microbiome can potentially alter pathogen strategies for acquiring iron. one of the most important transcriptional regulators controlling bacterial iron homeostasis is fur. here we us ... | 2016 | 27973608 |
| quantitative analysis of periodontal pathogens using real-time polymerase chain reaction (pcr). | the quantitative polymerase chain reaction (qpcr) is a variant of pcr aimed to detect and quantify a targeted dna molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated pcr product. this chapter presents the detailed procedures for quantification of different periodontal pathogens (porphyromonas gingivalis, aggregatibacter actinomycetemcomitans ... | 2017 | 27924595 |
| kr12 peptide associated with cyclodextrin: antimicrobial and antitumor activities. | the aim of this study was to determine the physical properties and antimicrobial and antiproliferative effects of the kr12 peptide complexed with 2-hydroxypropyl-β-cyclodextrin (hp-βcd) in vitro. the kr12:hp-βcd composition was evaluated for particle size and its zeta (ζ)-potential in the presence and absence of cells. antimicrobial activity against streptococcus mutans, actinobacillus actinomycetemcomitans, and porphyromonas gingivalis for the peptide alone or associated was evaluated by minima ... | 2016 | 27919160 |
| kr12 peptide associated with cyclodextrin: antimicrobial and antitumor activities. | the aim of this study was to determine the physical properties and antimicrobial and antiproliferative effects of the kr12 peptide complexed with 2-hydroxypropyl-β-cyclodextrin (hp-βcd) in vitro. the kr12:hp-βcd composition was evaluated for particle size and its zeta (ζ)-potential in the presence and absence of cells. antimicrobial activity against streptococcus mutans, actinobacillus actinomycetemcomitans, and porphyromonas gingivalis for the peptide alone or associated was evaluated by minima ... | 2016 | 27907988 |
| effect of resveratrol on periodontal pathogens during experimental periodontitis in rats. | the objective of this study was to investigate the antibacterial effect of resveratrol against putative periodontal pathogens during the progression of experimental periodontitis in rats. periodontitis was induced in rats in one of the first molars chosen to receive a ligature. animals were assigned to one of two groups: daily administration of the placebo solution (control group, n = 12) or 10 mg/kg of resveratrol (resv group, n = 12). the therapies were administered systemically for 30 days, f ... | 2016 | 27901209 |
| high-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. | periodontal disease (pd) is generated by microorganisms. these microbes can enter the general circulation causing a bacteraemia. the result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. level a evidence supports that pd is independently associated with arterial disease. pd is a common chronic condition affecting the majority of americans 30 years of age and older. atherosclerosis remains the largest cause of death and disability. studies indicate ... | 2017 | 27899684 |
| prevalence of periodontal pathogens as predictor of the evolution of periodontal status. | the aim of this study was to determine the relationship between the prevalence of porphyromonas gingivalis, its fima genotypes, aggregatibacter actinomycetemcomitans, tannerella forsythia, and treponema denticola and the evolution of periodontal health. in a longitudinal prospective study, samples of subgingival plaque were taken from 114 patients (37 with chronic periodontitis, 17 with gingivitis, and 60 periodontally healthy) in the course of a full periodontal examination. pcr was employed to ... | 2016 | 27888369 |
| detection and quantification of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and streptococcus oralis in blood samples with different microbiological identification methods: an in vitro study. | culture-based methods (culture broth bottles or lysis methods) have been the standard for detecting bacteremia. more recently, quantitative polymerase chain reaction (qpcr) was proposed as a more sensitive and specific test although none of them has been validated for the identification of periodontal pathogens (fastidious growing bacteria) in blood samples. | 2017 | 27875793 |
| nadph oxidase contributes to resistance against aggregatibacter actinomycetemcomitans-induced periodontitis in mice. | aggregatibacter actinomycetemcomitans is a gram-negative commensal bacterium of the oral cavity which has been associated with the pathogenesis of periodontitis with severe alveolar bone destruction. the role of host factors such as reactive oxygen and nitrogen intermediates in periodontal a. actinomycetemcomitans infection and progression to periodontitis is still ill-defined. therefore, this study aimed to analyze the role of nadph oxidase and inducible nitric oxide synthase (inos) in a murine ... | 2017 | 27849181 |
| nanofibers containing tetracycline/β-cyclodextrin: physico-chemical characterization and antimicrobial evaluation. | this study aimed to compare two nanofiber drug delivery systems that were prepared with an electrospun process and have the potential to serve as adjuvants for the treatment of periodontal disease. the first system was composed of polycaprolactone loaded with tetracycline (tcn) and the second was composed of polycaprolactone loaded with tetracycline/β-cyclodextrin (tcn:bcd). an antimicrobial diffusion test was performed for each of these sets of nanofibers with the microorganisms, aggregatibacte ... | 2017 | 27842841 |
| complete genome sequence of aggregatibacter actinomycetemcomitans strain idh781. | we report here the complete genomic sequence and methylome of aggregatibacter actinomycetemcomitans strain idh781. this rough strain is used extensively as a model organism to characterize localized aggressive periodontitis pathogenesis, the basic biology and oral cavity colonization of a. actinomycetemcomitans, and its interactions with other members of the oral microbiome. | 2016 | 27834722 |
| effects of aggregatibacter actinomycetemcomitans leukotoxin on neutrophil migration and extracellular trap formation. | aggressive periodontitis is associated with the presence of aggregatibacter actinomycetemcomitans, a leukotoxin (ltx)-producing periodontal pathogen. ltx has the ability to lyse white blood cells including neutrophils. | 2016 | 27834173 |
| thermoreversible gel for intrapocket delivery of green tea catechin as a local drug delivery system: an original research. | the periodontal therapies along with systemic antibiotic therapy aim at eliminating the subgingival microbiota to arrest the progression of periodontal diseases. the complete elimination is often difficult, and thus the probability of repopulation after periodontal therapy is also high. the objectives of the study are to develop in situ thermoreversible gelling system of green tea catechins suitable for periodontal pocket administration, which would act as an adjunct to mechanical periodontal th ... | 2017 | 27833893 |
| periodontitis and myocardial hypertrophy. | there is a deep relationship between cardiovascular disease and periodontitis. it has been reported that myocardial hypertrophy may be affected by periodontitis in clinical settings. although these clinical observations had some study limitations, they strongly suggest a direct association between severity of periodontitis and left ventricular hypertrophy. however, the detailed mechanisms between myocardial hypertrophy and periodontitis have not yet been elucidated. recently, we demonstrated tha ... | 2017 | 27829660 |
| influence of culture conditions on porphyrin production in aggregatibacter actinomycetemcomitans and porphyromonas gingivalis. | increasing antibiotic resistance among pathogens has raised the demands for new treatment methods such as antimicrobial photodynamic therapy (apdt) and phototherapy (pt). experiments for investigating the effects of these methods are often performed in vitro, but the procedures for cultivation of microbes vary between different studies. the aim of this study has been to elucidate how the profile of endogenously produced porphyrins differs by changing the variables of bacteria culturing condition ... | 2017 | 27825899 |
| regulation of matrix metalloproteinase secretion by green tea catechins in a three-dimensional co-culture model of macrophages and gingival fibroblasts. | elevated levels of matrix metalloproteinases (mmps) have been associated with the active phases of tissue and bone destruction in periodontitis, an inflammatory disease characterized by a significant breakdown of tooth support. in the present study, we used a three-dimensional (3d) co-culture model of macrophages and gingival fibroblasts to investigate the ability of a green tea extract and its major constituent epigallocatechin-3-gallate (egcg) to regulate the secretion of mmp-3, -8, and -9. | 2017 | 27825679 |
| microbiological and clinical assessment of the abutment and non-abutment teeth of partial removable denture wearers. | the aim of this study was assessing the changes in both clinical and microbiological parameters of healthy individuals after rehabilitation with removable partial denture (rpd). | 2017 | 27825678 |
| assessment of photodynamic inactivation against periodontal bacteria mediated by a chitosan hydrogel in a 3d gingival model. | chitosan hydrogels containing hydroxypropyl methylcellulose (hpmc) and toluidine blue o were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (pdi). increased hpmc content in the hydrogels resulted in increased mucoadhesiveness. furthermore, we developed a simple in vitro 3d gingival model resembling the oral periodontal pocket to culture the biofilms of staphylococcus aureus (s. aureus), aggregatibacter actinomycetemcomitans (a. actin ... | 2016 | 27809278 |
| localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva. | the purpose of this study is to elucidate the localization of amelotin (amtn), odontogenic ameloblast-associated protein (odam) and follicular dendritic cell-secreted protein (fdc-sp) at the junctional epithelium (je) in porphyromonas gingivalis and aggregatibacter actinomycetemcomitans infected mice and inflamed and non-inflamed human gingiva. we performed immunostaining to determine the localization and expression pattern of amtn, odam and fdc-sp. amtn, odam and fdc-sp in a. actinomycetemcomit ... | 2016 | 27807653 |
| human oral microbiome and prospective risk for pancreatic cancer: a population-based nested case-control study. | a history of periodontal disease and the presence of circulating antibodies to selected oral pathogens have been associated with increased risk of pancreatic cancer; however, direct relationships of oral microbes with pancreatic cancer have not been evaluated in prospective studies. we examine the relationship of oral microbiota with subsequent risk of pancreatic cancer in a large nested case-control study. | 2016 | 27742762 |
| mitogen-activated protein kinase 2 signaling shapes macrophage plasticity in aggregatibacter actinomycetemcomitans-induced bone loss. | aggregatibacter actinomycetemcomitans is associated with aggressive periodontal disease, which is characterized by inflammation-driven alveolar bone loss. a. actinomycetemcomitans activates the p38 mitogen-activated protein kinase (mapk) and mapk-activated protein kinase 2 (mk2) stress pathways in macrophages that are involved in host responses. during the inflammatory process in periodontal disease, chemokines are upregulated to promote recruitment of inflammatory cells. the objective of this s ... | 2017 | 27795356 |
| periodontal and microbiological profile of intensive care unit inpatients. | the bidirectional relationship between the periodontal diseases and systemic diseases was attributed to the focal infection concept. the aims of this study were to assess the periodontal and microbiological profile of intensive care unit (icu) inpatients submitted to orotracheal intubation, and classify them regarding gender, age group, ethnic, hospitalization reason and period, nosocomial infection occurrence, and death. | 2016 | 27794150 |
| changes in salivary periodontal pathogens after orthodontic treatment: an in vivo prospective study. | to analyze the initial changes in salivary levels of periodontal pathogens after orthodontic treatment with fixed appliances. | 2016 | 27792427 |
| [a case of mediastinum actinomycosis by aggregatibacter actinomycetemcomitans]. | the actinomycosis is a suppurative infection due to an anaerobic and microaerophillic bacteria called actinomyces. only few case reports are described for the mediastinal locations of this rare entity. we report a new case of inflammatory pseudotumor in the mediastinum due to aggregatibacte actinomycetemcomitans revealed by hemoptysis. the mediastinoscopy procedure with biopsy was needed to confirm the definitive bacteriological diagnosis by a positive culture. during the postoperative course, a ... | 2016 | 27789164 |
| comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample? | the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control). | 2017 | 27786617 |
| does obesity influence the subgingival microbiota composition in periodontal health and disease? | to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp). | 2016 | 27717180 |
| association of porphyromonas gingivalis with high levels of stress-induced hormone cortisol in chronic periodontitis patients. | the aim of the present study was to evaluate the association between the occurrence of periodontopathogens with cortisol levels in chronic periodontitis patients. | 2016 | 26194628 |
| mobilization of tlr4 into lipid rafts by aggregatibacter actinomycetemcomitans in gingival epithelial cells. | an investigation of the mechanisms underlying the production of inflammatory cytokines through the stimulation of microorganisms on gingival epithelial cells may provide insights into the pathogenesis of the initiation of periodontitis. lipid rafts, microdomains in the cell membrane, include a large number of receptors, and are centrally involved in signal transduction. we herein examined the involvement of lipid rafts in the expression of interleukin (il-6) and il-8 in gingival epithelial cells ... | 2016 | 27744428 |
| rosuvastatin inhibits interleukin (il)-8 and il-6 production in human coronary artery endothelial cells stimulated with aggregatibacter actinomycetemcomitans serotype b. | rosuvastatin exhibits anti-inflammatory effects and reduces periodontal diseases and atherosclerosis; however, its role in regulating periodontopathogen-induced endothelial proinflammatory responses remains unclear. the purpose of this study is to determine whether rosuvastatin can reduce the proinflammatory response induced by aggregatibacter actinomycetemcomitans (aa) in human coronary artery endothelial cells (hcaecs). | 2017 | 27739345 |
| bacterial heat shock protein groel (hsp64) exerts immunoregulatory effects on t cells by utilizing apoptosis. | aggregatibacter actinomycetemcomitans (aa) expresses a 64-kda groel protein belonging to the heat shock family of proteins. this protein has been shown to influence human host cells, but the apoptotic capacity of the groel protein regarding t cells is not yet known. the purpose of this study was to investigate the ability of a. actinomycetemcomitans groel (aagroel) protein to induce human peripheral blood t-cell apoptosis. endogenous, purified aagroel protein was used as an antigen. in aagroel-t ... | 2016 | 27736933 |
| association between infectious burden, socioeconomic status, and ischemic stroke. | infectious diseases contribute to stroke risk, and are associated with socioeconomic status (ses). we tested the hypotheses that the aggregate burden of infections increases the risk of ischemic stroke (is) and partly explains the association between low ses and ischemic stroke. | 2016 | 27728851 |
| irradiation effects of low temperature multi gas plasma jet on oral bacteria. | the purpose of this study is to evaluate the sterilization effects of a newly developed low temperature multi gas plasma jet on oral pathogenic microorganisms (streptococcus mutans [s. mutans], lactobacillus fermentum [l. fermentum], aggregatibacter actinomycetemcomitans [a. actinomycetemcomitans]). plasma gas which generated from o2, n2, ar and 50% (o2+n2) was irradiated to the microbes. effect of o2 plasma irradiation on s. mutans under scanning electron microscopy (sem) was also observed. o2 ... | 2016 | 27725521 |
| structural and functional analysis of de-n-acetylase pgab from periodontopathogen aggregatibacter actinomycetemcomitans. | the oral pathogen aggregatibacter actinomycetemcomitans uses pga gene locus for the production of an exopolysaccharide made up of a linear homopolymer of β-1,6-n-acetyl-d-glucosamine (pga). an enzyme encoded by the pgab of the pga operon in a. actinomycetemcomitans is a de-n-acetylase, which is used to alter the pga. the full length enzyme (aapgab) and the n-terminal catalytic domain (residues 25-290, aapgabn) from a. actinomycetemcomitans were cloned, expressed and purified. the enzymatic activ ... | 2016 | 27706922 |
| effect of bioactive dental adhesive on periodontal and endodontic pathogens. | the objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. nanoparticles of amorphous ca ... | 2016 | 27699574 |
| outer membrane protein 100 of aggregatibacter actinomycetemcomitans act as a biopharmaceutical target for photodynamic therapy: an in silico analysis. | periodontitis is a polymicrobial, chronic, and degenerative disease that can lead to destruction of the teeth-supporting tissues and eventually to loss of teeth. aggregatibacter actinomycetemcomitans is predominantly associated with periodontal diseases. outer membrane protein (omp) 100 is a more important virulence factor of a. actinomycetemcomitans due to the effect of adhesion and invasion into human gingival epithelial cells. attachment of a. actinomycetemcomitans inhibition is significant i ... | 2016 | 27697516 |
| evaluation of the antibacterial potential of liquid and vapor phase phenolic essential oil compounds against oral microorganisms. | the aim of the present study was to determine the antibacterial activities of the phenolic essential oil (eo) compounds hinokitiol, carvacrol, thymol, and menthol against oral pathogens. aggregatibacter actinomycetemcomitans, streptococcus mutans, methicillin-resistant staphylococcus aureus (mrsa), and escherichia. coli were used in this study. the minimum inhibitory concentrations (mics), minimum bactericidal concentrations (mbcs), bacterial growth curves, temperature and ph stabilities, and sy ... | 2017 | 27681039 |
| novel bioactive nanocomposite for class-v restorations to inhibit periodontitis-related pathogens. | the occurrence of tooth root caries is increasing as the world population ages and tooth retention in seniors increases. class v restorations with subgingival margins are difficult to clean and often lead to periodontitis. the objectives of this study were to develop a class v composite containing dimethylaminohexadecyl methacrylate (dmahdm) and nanoparticles of amorphous calcium phosphate (nacp), and investigate mechanical properties and the inhibition of six species of periodontitis-related bi ... | 2016 | 27671471 |
| microbial characteristics of peri-implantitis: a case-control study. | the aim of this case-control study is to compare oral microbiologic characteristics of patients with healthy peri-implant conditions and patients with peri-implantitis and to explore the influence of various patient- and implant-related factors on microbiologic characteristics. | 2017 | 27666672 |
| novel strategy to detect and locate periodontal pathogens: the pna-fish technique. | we aim to develop peptic nucleic acid (pna) probes for the identification and localization of aggregatibacter actinomycetemcomintans and porphyromonas gingivalis in sub-gingival plaque and gingival biopsies by fluorescence in situ hybridization (fish). | 2016 | 27664736 |
| in vitro complement activation, adherence to red blood cells and induction of mononuclear cell cytokine production by four strains of aggregatibacter actinomycetemcomitans with different fimbriation and expression of leukotoxin. | the periodontal pathogen aggregatibacter actinomycetemcomitans has been proposed as pro-atherogenic, and complement-mediated adherence to red blood cells (rbcs) may facilitate its systemic spread. we investigated the ability of four strains of a. actinomycetemcomitans with differential expression of leukotoxin a (ltxa) and fimbriae to activate complement, adhere to rbcs and elicit cytokine responses by mononuclear cells (mncs). | 2016 | 27663487 |
| innate immune response of human embryonic stem cell-derived fibroblasts and mesenchymal stem cells to periodontopathogens. | periodontitis involves complex interplay of bacteria and host immune response resulting in destruction of supporting tissues of the tooth. toll-like receptors (tlrs) play a role in recognizing microbial pathogens and eliciting an innate immune response. recently, the potential application of multipotent stem cells and pluripotent stem cells including human embryonic stem cells (hescs) in periodontal regenerative therapy has been proposed. however, little is known about the impact of periodontopa ... | 2016 | 27642305 |
| physicochemical fabrication of antibacterial calcium phosphate submicrospheres with dispersed silver nanoparticles via coprecipitation and photoreduction under laser irradiation. | we achieved rapid, surfactant-free, and one-pot fabrication of antibacterial calcium phosphate (cap) submicrospheres containing silver nanoparticles by combining physical laser and chemical coprecipitation processes. in this physicochemical process, weak pulsed laser irradiation (20min) was performed on a labile cap reaction mixture supplemented with silver ions as a light-absorbing agent. the silver content in the submicrospheres was controlled for a wide range (ag/p elemental ratio varied from ... | 2016 | 27640919 |