Publications

structures of ribonuclease p rnas of vibrio core species.	the structures of an rna component of ribonuclease p (rnase p rna) were examined for vibrio parahaemolyticus, vibrio alginolyticus, vibrio carchariae, vibrio natriegens, vibrio campbellii, vibrio proteolyticus, vibrio pelagius and vibrio harveyi to clearly determine their genetic differences. the rnase p rnas ranged from 382 to 454 nucleotides (nt) in size, and were remarkably different from each other in the structure of two helices, p3 and p12. the p3 helices were comprised of tandem repeats o ...	2001	11430405
fourier transform-infrared spectroscopic methods for microbial ecology: analysis of bacteria, bacteria-polymer mixtures and biofilms.	fourier transform-infrared (ft-ir) spectroscopy has been used to rapidly and nondestructively analyze bacteria, bacteria-polymer mixtures, digester samples and microbial biofilms. diffuse reflectance ft-ir (drift) analysis of freeze-dried, powdered samples offered a means of obtaining structural information. the bacteria examined were divided into two groups. the first group was characterized by a dominant amide i band and the second group of organisms displayed an additional strong carbonyl ...	1985	11539050
n2 fixation in marine heterotrophic bacteria: dynamics of environmental and molecular regulation.	molecular and immunological techniques were used to examine n2 fixation in a ubiquitous heterotrophic marine bacterium, the facultative anaerobic vibrio natriegens. when batch cultures were shifted from aerobic n-replete to anaerobic n-deplete conditions, transcriptional and post-translational regulation of n2 fixation was observed. levels of nifhdk mrna encoding the nitrogenase enzyme were highest at 140 min postshift and undetectable between 6 and 9 h later. immunologically determined levels o ...	1996	11607653
rrna promoter activity in the fast-growing bacterium vibrio natriegens.	the bacterium vibrio natriegens can double with a generation time of less than 10 min (r. g. eagon, j. bacteriol. 83:736-737, 1962), a growth rate that requires an extremely high rate of protein synthesis. we show here that v. natriegens' high potential for protein synthesis results from an increase in ribosome numbers with increasing growth rate, as has been found for other bacteria. we show that v. natriegens contains a large number of rrna operons, and its rrna promoters are extremely strong. ...	2002	11844764
reca expression in response to solar uvr in the marine bacterium vibrio natriegens.	solar ultraviolet radiation may produce daily stress on marine and estuarine communities as cells are damaged and repair that damage. reduction in the earth's stratospheric ozone layer has increased awareness of the potential effects that ultraviolet radiation may have in the environment, including how marine bacteria respond to changes in solar radiation. we examined the use of the bacterial reca protein as an indicator of the potential of bacteria to repair dna damage caused by solar uv irradi ...	2001	12024236
estimation of biologically damaging uv levels in marine surface waters with dna and viral dosimeters.	we have surveyed the biologically harmful radiation penetrating the water column along a transect in the western gulf of mexico using dosimeters consisting of intact viruses or naked calf-thymus dna (ctdna). the indigenous marine bacteriophage pwh3a-p1, which lytically infects the heterotrophic bacterium vibrio natriegens (strain pwh3a), displayed decay rates for infectivity approaching 1.0 h(-1) in surface waters when deployed in a seawater-based dosimeter. the accumulation of pyrimidine dimers ...	2002	12403447
[biosynthesis and accumulation of poly(3-hydroxybutyrate) in vibrio natriegens].	accumulation of poly(3-hydroxybutyrate) [poly(3hb)] by v. natriegens was studied. results indicated that v. natriegens used glucose, gluconate, fructose and molasses as carbon sources for poly(3hb) synthesis. when molasses was used, up to 28.4% of poly(3hb) to cellular dry weight was accumulated. the accumulation of poly(3hb) followed, was not simultaneously to, the cell growth. analysis of the pha polymerase, beta-ketothiolase, and acetoacetyl-coa reductase showed that the poly(3hb) accumulatio ...	2002	12561210
some observations on the physiology of pseudomonas natriegens nov. spec.		1961	13733692
dissimilation of glucose and gluconic acid by pseudomonas natriegens.	eagon, r. g. (university of georgia, athens) and c. h. wang. dissimilation of glucose and gluconic acid by pseudomonas natriegens. j. bacteriol. 83:879-886. 1962-when glucose dissimilation of a marine pseudomonad, pseudomonas natriegens, was studied, enzymes of both the glycolytic pathway and of the hexose monophosphate pathway were detected in extracts of glucose-grown cells. enzymes of the entner-doudoroff pathway and phosphoketolase were not detected. data from radiorespirometric experiments ...	1962	13888944
pseudomonas natriegens, a marine bacterium with a generation time of less than 10 minutes.	eagon, r. g. (university of georgia, athens). pseudomonas natriegens, a marine bacterium with a generation time of less than 10 minutes. j. bacteriol. 83:736-737. 1962.-pseudomonas natriegens, a marine microorganism, was demonstrated to have a generation time of 9.8 min. this is the shortest generation time reported to date. optimal growth occurred at 37 c in brain heart infusion broth supplemented with 1.5% sea salt.	1962	13888946
lactic dehydrogenases of pseudomonas natriegens.	walker, hazel (university of georgia, athens), and r. g. eagon. lactic dehydrogenases of pseudomonas natriegens. j. bacteriol. 88:25-30. 1964.-lactic dehydrogenases specific for d- and l-lactate were demonstrated in pseudomonas natriegens. the l-lactic dehydrogenase showed considerable heat stability, and 40% of the activity remained in extracts after heating at 60 c for 10 min. an essential thiol group for enzyme activity was noted. the results of these experiments were consistent with the view ...	1964	14197895
major products of glucose dissimilation by pseudomonas natriegens.	eagon, r. g. (university of georgia, athens), and h. w. cho. major products of glucose dissimilation by pseudomonas natriegens. j. bacteriol. 89:1209-1211. 1965.-pseudomonas natriegens aerobically catabolized glucose to yield predominantly acetic acid, pyruvic acid, and co(2), whereas little or no lactic acid was formed. under anaerobic conditions, glucose in an enriched medium was fermented to yield acetic acid and lactic acid but no pyruvic acid or co(2). glucose in a basal salts medium was fe ...	1965	14292987
fluorescently labeled virus probes show that natural virus populations can control the structure of marine microbial communities.	fluorescently stained viruses were used as probes to label, identify, and enumerate specific strains of bacteria and cyanobacteria in mixed microbial assemblages. several marine virus isolates were fluorescently stained with yoyo-1 or popo-1 (molecular probes, inc.) and added to seawater samples that contained natural microbial communities. cells to which the stained viruses adsorbed were easily distinguished from nonhost cells; typically, there was undetectable binding of stained viruses to nat ...	1995	16535146
pyridine nucleotide-linked reactions of pseudomonas natriegens.	eagon, r. g. (university of georgia, athens). pyridine nucleotide-linked reactions of pseudomonas natriegens. j. bacteriol. 84:819-821. 1962-the observation that pseudomonas natriegens utilizes the embden-meyerhof pathway and the hexose monophosphate-pentose cycle only very slightly, even though the necessary enzymes are present, was explained by the existence of a sluggish system for the oxidation of reduced triphosphopyridine nucleotide (tpnh). pyridine nucleotide transhydrogenase could not be ...	1962	16561966
plasticity of the gene functions for dna replication in the t4-like phages.	we have completely sequenced and annotated the genomes of several relatives of the bacteriophage t4, including three coliphages (rb43, rb49 and rb69), three aeromonas salmonicida phages (44rr2.8t, 25 and 31) and one aeromonas hydrophila phage (aeh1). in addition, we have partially sequenced and annotated the t4-like genomes of coliphage rb16 (a close relative of rb43), a. salmonicida phage 65, acinetobacter johnsonii phage 133 and vibrio natriegens phage nt-1. each of these phage genomes exhibit ...	2006	16828113
production of poly-beta-hydroxybutyrate (phb) by vibrio spp. isolated from marine environment.	bacteria isolated from marine sediments were screened for their ability to accumulate polyhydroxyalkanoates. among the isolates, four vibrio spp. (strain m11, m14, m20 and m31) were studied in detail. all synthesized intracellular lipid inclusions during growth on diverse carbon sources including acetate, glycerol, succinate, glucose and sucrose. the inclusions were identified to be poly-beta-hydroxybutyrate (phb) using gas chromatography and nuclear magnetic resonance analysis. no other type of ...	2007	17416432
effects of ultraviolet radiation on the gram-positive marine bacterium microbacterium maritypicum.	although extensive information is available on the effect ultraviolet (uv) radiation has on gram-negative marine bacteria, there is a scarcity of data concerning uv radiation and gram-positive marine bacteria. the focus of this paper is on microbacterium maritypicum, with the gram-negative vibrio natriegens being used as a standard of comparison. m. maritypicum exhibited growth over a nacl range of 0-1000 mm: , with optimum growth occurring between 0 and 400 mm: nacl. in contrast, v. natriegens ...	2007	17551790
specific atp and specific oxygen uptake rate in immobilized cell aggregates: experimental results and theoretical analysis using a structured model of immobilized cell growth.	in this work the quality and activity of immobilized beneckea natriegens have been measured using specific atp (satp) [mg (atp) g(-1) (dry biomass)] and specific oxygen uptake rate (sour) [mg o(2) g(-1) (dry biomass) h(-1)]. the cells were grown in a 3 l three phase air lift bioreactor (tpalb) and were immobilized on diatomaceous earth (silica) support particles; sterile conditions were employed during the experiment, with n-propanol as the sole carbon source. two sets of experiments were perfor ...	1997	18636576
the respiratory system of the marine bacterium beneckea natriegens. i. cytochrome composition.	(1) the cytochrome composition of beneckea natriegens grown under aerobic conditions has been examined. (2) cell-free extracts obtained by sonication were separated into particulate and supernatant fractions by centrifugation at 150,000 x g. (3) the particulate fraction contained cytochromes b562, b557, b or c554, c549.5, c547, and low concentrations of cytochromes a1 and a2. (subscripts refer to the wavelength optima of the b and c type cytochrome alpha-peaks in low temperature (77 degrees k) d ...	1974	19400035
marine bacterioplankton can increase evaporation and gas transfer bymetabolizing insoluble surfactants from the air-seawater interface.	hydrophobic surfactants at the air-sea interface can retard evaporative and gaseous exchange between the atmosphere and the ocean.while numerous studies have examined the metabolic role of bacterioneuston at the air-sea interface, the interactions between hydrophobic surfactants and bacterioplankton are not well constrained. a novel experimental design was developed, using vibrio natriegens and (3)h-labelled hexadecanoic acid tracer, to determine how the bacterial metabolism of fatty acids affec ...	2009	19431239
antibacterial activity and qsar of chalcones against biofilm-producing bacteria isolated from marine waters.	biofouling in the marine environment is a major problem. in this study, three marine organisms, namely bacillus flexus (ld1), pseudomonas fluorescens (md3) and vibrio natriegens (md6), were isolated from biofilms formed on polymer and metal surfaces immersed in ocean water. phylogenetic analysis of these three organisms indicated that they were good model systems for studying marine biofouling. the in vitro antifouling activity of 47 synthesized chalcone derivatives was investigated by estimatin ...	2010	20544550
2-methoxy-2',4'-dichloro chalcone as an antimicrofoulant against marine bacterial biofilm.	marine paint mixed with 2-methoxy-2',4'-dichloro chalcone is able to considerably reduce the formation of biofilm by vibrio natriegens, a marine bacterium, on polycarbonate (pc), polymethylmethacrylate (pmma) and glass fiber reinforced plastic (gfrp). these polymers have been selected for the study, since they have wide marine applications. surfaces coated with dichloro chalcone containing marine paint had the lowest number of colony forming units (cfu) (1-5×10(6)), proteins (20-30 μg/cm2) and c ...	2010	20708908
identification of vibrio natriegens uvra and uvrb genes and analysis of gene regulation using transcriptional reporter plasmids.	nucleotide excision repair (ner) rectifies a variety of chemically and structurally distinct dna lesions. the current model of ner is based upon the enteric bacterium escherichia coli and there is scarce information about how other bacterial species respond to, and correct, dna damage. here we report the isolation and functional analysis of the uvra and uvrb genes from vibrio natriegens, a naturally occurring marine bacterium. genetic studies were completed to assess the repair capabilities of v ...	2010	21046343
[genotypic analysis and plant growth-promoting ability of four plant growth-promoting bacteria from mangrove].	we identified four strains of plant growth-promoting bacteria (pgpb) and their plant growth-promoting ability.	2010	21141471
detection of quorum sensing signal molecules in the family vibrionaceae.	the aim of this study was to detect the production of three kinds of quorum sensing (qs) signal molecules, i.e. the n-acyl-homoserine lactone (ahl), the autoinducer-2 (ai-2) and the cholerae autoinducer-1-like (cai-1-like) molecules in 25 vibrionaceae strains.	2011	21395950
Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon).	Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ...	2011	21984666
Production and characterization of a novel monoclonal antibody against Vibrio parahaemolyticus F(0)F(1) ATP synthase's delta subunit and its application for rapid identification of the pathogen.	We raised monoclonal antibodies (MAbs) against Vibrio parahaemolyticus cell extracts. One of the MAbs, designated MAb-VP34, reacted in enzyme-linked immunosorbent assays (ELISAs) with 140 V. parahaemolyticus strains, regardless of serotype or origin. MAb-VP34 did not detectably react with 96 strains belonging to 27 other Vibrio species (except for Vibrio natriegens) or with 29 non-Vibrio species. These results show that MAb-VP34 is highly specific for V. parahaemolyticus. Western blotting and ma ...	2012	22056232
draft genome sequence of the fast-growing marine bacterium vibrio natriegens strain atcc 14048.	vibrio natriegens bacteria are gram-negative aquatic microorganisms that are found primarily in coastal seawater and sediments and are perhaps best known for their high growth rates (generation time of <10 min). in this study, we report the first sequenced genome of this species, that of the type strain vibrio natriegens atcc 14048, a salt marsh mud isolate from sapelo island, ga.	2013	23929482
draft genome sequence of the fast-growing bacterium vibrio natriegens strain dsmz 759.	vibrio natriegens is a gram-negative bacterium known for its extremely short doubling time. here we present the annotated draft genome sequence of vibrio natriegens strain dsmz 759, with the aim of providing insights about its high growth rate.	2013	23969053
the mosaic accessory gene structures of the sxt/r391-like integrative and conjugative elements derived from vibrio spp. isolated from aquatic products and environment in the yangtze river estuary, china.	the emergence, resurgence and spread of human food-borne pathogenic vibrios are one of the major contributors to disease burden and mortality particularly in developing countries with disputable sanitary conditions. previous research on pathogenic vibrio cholerae and vibrio parahaemolitycus derived from clinical samples has proposed links between acquisition of virulence and multiple drug resistance traits and intercellular transmissibility of mobile genetic elements in the environment. to date, ...	2013	24074349
an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis.	c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ...	2014	24140432
genes similar to the vibrio parahaemolyticus virulence-related genes tdh, tlh, and vscc2 occur in other vibrionaceae species isolated from a pristine estuary.	detection of the human pathogen vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. these genes have been employed in determinations of v. parahaemolyticus population numbers and the prevalence of pathogenic v. parahaemolyticus strains. strains of the vibrionaceae species photobacterium damselae, vibrio diabolicus, vibrio harveyi, and vibrio natriegens, as well as strains similar to vibrio tubiashii, were isolated from a pristine salt ...	2014	24212573
function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis.	c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ...	2014	24561128
universally improving effect of mixed electron donors on the co₂ fixing efficiency of non-photosynthetic microbial communities from marine environments.	the universality of improved co₂ fixing upon the addition of mixed electron donors (meds) composed of na₂s, no₂(-), and s₂o₃(2-) to non-photosynthetic microbial communities (npmcs) obtained from 12 locations in four oceans of the world was validated. the co₂ fixing efficiencies of npmcs were universally enhanced by med compared with those obtained using h₂ alone as electron donor, with average increase of about 276%. an increase in microbial inoculation concentration could increase the net amoun ...	2014	25108727
sulfation mediates activity of zosteric acid against biofilm formation.	zosteric acid (za), a metabolite from the marine sea grass zostera marina, has attracted much attention due to its attributed antifouling (af) activity. however, recent results on dynamic transformations of aromatic sulfates in marine phototrophic organisms suggest potential enzymatic desulfation of metabolites like za. the activity of za was thus re-investigated using biofilm assays and simultaneous analytical monitoring by liquid chromatography/mass spectrometry (lc/ms). comparison of za and i ...	2015	25915112
a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis).	integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ...	2015	26004499
sulfated phenolic acids from dasycladales siphonous green algae.	sulfated aromatic acids play a central role as mediators of chemical interactions and physiological processes in marine algae and seagrass. among others, dasycladus vermicularis (scopoli) krasser 1898 uses a sulfated hydroxylated coumarin derivative as storage metabolite for a protein cross linker that can be activated upon mechanical disruption of the alga. we introduce a comprehensive monitoring technique for sulfated metabolites based on fragmentation patterns in liquid chromatography/mass sp ...	2015	26188914
[screening and identification of a bacterium capable of converting agar to neoagaro oligosaccharides].	to screen and identify a bacterium capable of converting agar to neoagaro oligosaccharides.	2015	26762025
construction of a bioreporter by heterogeneously expressing a vibrio natriegens reca::luxcdabe fusion in escherichia coli, and genotoxicity assessments of petrochemical-contaminated groundwater in northern china.	here, we constructed an escherichia coli reca::luxcdabe bioreporter for genotoxicity assessments. the reca promoter was cloned from the marine bacterium vibrio natriegens. this bioreporter showed a dose-response relationship following induction by mitomycin c, and other pollutants or environmental samples could be calculated as mitomycin c equivalents, which provided a way to quantitatively compare the genotoxicities of different environmental samples. this bioreporter was used to evaluate the g ...	2016	27258332
vibrio natriegens as a fast-growing host for molecular biology.	a rapidly growing bacterial host would be desirable for a range of routine applications in molecular biology and biotechnology. the bacterium vibrio natriegens has the fastest growth rate of any known organism, with a reported doubling time of <10 min. we report the development of genetic tools and methods to engineer v. natriegens and demonstrate the advantages of using these engineered strains in common biotech processes.	2016	27571549
multiplex genome editing by natural transformation (mugent) for synthetic biology in vibrio natriegens.	vibrio natriegens has recently emerged as an alternative to escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. here, we uncover how to induce natural competence in v. natriegens and describe methods for multiplex genome editing by natural transformation (mugent). mugent promotes integration of multiple genome edits at high-efficiency on unprecedented time scales. also, this method allows for generating highly complex mutant populati ...	2017	28571309
environmental impact of tributyltin-resistant marine bacteria in the indigenous microbial population of tributyltin-polluted surface sediments.	we compared the tbt-resistant ability of resting cells prepared from isolates that formed colonies on nutrient agar plates containing 100 µm tributyltin (tbt) chloride, such as photobacterium sp. tky1, halomonas sp. tky2, and photobacterium sp. ngy1, with those from taxonomically similar type strains. photobacterium sp. tky1 showed the highest ability among those three isolates. the number of surviving photobacterium sp. tky1 cells was hardly decreased after 1 h of exposure to 100 µm tbtcl, rega ...	2017	28659560