Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| rapid and sensitive quantification of vibrio cholerae and vibrio mimicus cells in water samples by use of catalyzed reporter deposition fluorescence in situ hybridization combined with solid-phase cytometry. | a new protocol for rapid, specific, and sensitive cell-based quantification of vibrio cholerae/vibrio mimicus in water samples was developed. the protocol is based on catalyzed reporter deposition fluorescence in situ hybridization (card-fish) in combination with solid-phase cytometry. for pure cultures, we were able to quantify down to 6 v. cholerae cells on one membrane with a relative precision of 39% and down to 12 cells with a relative precision of 17% after hybridization with the horseradi ... | 2012 | 22885749 |
| identification and immunogenicity of immunodominant mimotopes of outer membrane protein u (ompu) of vibrio mimicus from phage display peptide library. | vibrio mimicus (v. mimicus) is the causative agent of ascites disease in aquatic animals. outer membrane protein u (ompu) is an important antigen of v. mimicus, but its protective epitopes are still unclear. a random 12-mer phage-displayed peptide library was used to screen and identify immunodominant mimotopes of the ompu protein in v. mimicus by panning against purified ompu-specific polyclonal antibody. then the immunogenicity and immunoprotection in fish of these mimotopes was evaluated. nin ... | 2013 | 23178262 |
| genome sequence and comparative genomics analysis of a vibrio cholerae o1 strain isolated from a cholera patient in malaysia. | the genome sequence analysis of a clinical vibrio cholerae vc35 strain from an outbreak case in malaysia indicates multiple genes involved in host adaptation and a novel na(+)-driven multidrug efflux pump-coding gene in the genome of vibrio cholerae with the highest similarity to vma_001754 of vibrio mimicus vma223. | 2012 | 23209200 |
| draft genome sequence of vibrio mimicus strain caim 602t. | vibrio mimicus is a gram-negative bacterium associated with gastrointestinal diseases in humans around the world. we report the complete genome sequence of the vibrio mimicus strain caim 602(t) (cdc1721-77, lmg 7896(t), atcc 33653(t)). | 2013 | 23516211 |
| hemolytic and urease activities in vibrios isolated from fresh and frozen oysters. | the present study aimed to survey the vibrio microbiota of oysters (crassostrea rhizophorae) obtained from restaurants in fortaleza, state of ceará, brazil, and to identify virulence factors. | 2013 | 23563836 |
| dietary organic selenium improves growth, survival and resistance to vibrio mimicus in cultured marron, cherax cainii (austin, 2002). | to determine the effects of dietary organic selenium (os) supplementation on the growth performance and immune competence of marron, cherax cainii (austin, 2002), a group of marron were fed 0.2 g kg(-1) of sel-plex(®) supplemented basal diet and then compared with another group (control) of marron fed basal diet without any supplementation. after 90 days of feeding, final weight, average weekly gains (awg), relative gain rate (rgr), specific growth rate (sgr), survival, total and differential ha ... | 2013 | 23603239 |
| in vitro and in vivo bactericidal activity of vitex negundo leaf extract against diverse multidrug resistant enteric bacterial pathogens. | to investigate in vitro and in vivo antibacterial potentials of vitex negundo (v. negundo) leaf extracts against diverse enteric pathogens. | 2013 | 23608373 |
| immunological responses of customised probiotics-fed marron, cherax tenuimanus, (smith 1912) when challenged with vibrio mimicus. | a two-phased experiment was conducted to investigate the effects of dietary supplementation of customised probiotics on marron physiology. during the first phase marron were fed probiotic supplemented feed for 70 days, while in phase two the same marron were challenged with vibrio mimicus and their physiological responses were investigated for 4 days post-challenged. the experiment was carried out in a purpose-built room, designed for aquaculture research, using 18 of 250 l cylindrical plastic t ... | 2013 | 23643874 |
| development of a selective and differential medium for capsulated lactococcus garvieae. | a selective and differential medium termed 'lg agar' was developed for the isolation and presumptive identification of lactococcus garvieae that results in black colonies with red halos. in this study, all 14 strains of l. garvieae and only 9 of the 148 strains representing 38 other species were able to grow on the lg agar. the nine viable strains on lg agar plates (including enterococcus faecalis, enterococcus faecium, lactococcus lactis, vibrio fluvialis, vibrio furnissii, vibrio mimicus and v ... | 2014 | 24033791 |
| relationship of aquatic environmental factors with the abundance of vibrio cholerae, vibrio parahaemolyticus, vibrio mimicus and vibrio vulnificus in the coastal area of guaymas, sonora, mexico. | members of the genus vibrio are common in aquatic environments. among them are v. cholerae, v. vulnificus, v. parahaemolyticus and v. mimicus. several studies have shown that environmental factors, such as temperature, salinity, and dissolved oxygen, are involved in their epidemiology. therefore, the main objective of this study is to determine if there is a correlation between the presence/amount of v. cholerae, v, vulnificus, v. parahaemolyticus and v. mimicus and the environmental conditions ... | 2013 | 24334844 |
| vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus vibrio. | using a 0.27 kb dna probe specific for the heat-stable enterotoxin gene (nag-st) of vibrio cholerae non-o1, 1109 strains representing 17 species of the genus vibrio, isolated from clinical and environmental sources were examined. the nag-st gene was preponderantly associated with strains classified as v. mimicus; 16.8% of these strains hybridized. it was more frequent in the clinical isolates (22.6%) than in the environmental isolates (13.7%). the incidence of nag-st gene-positive strains of v. ... | 1994 | 24420888 |
| vibrio parahaemolyticus and its specific bacteriophages as an indicator in cockles (anadara granosa) for the risk of v. parahaemolyticus infection in southern thailand. | correlation between the numbers of vibrio parahaemolyticus and its specific bacteriophages in cockles was investigated from june 2009 to may 2010 in hat yai, songkhla, thailand. cockles obtained monthly from a local market were sampled to determine the numbers of v. parahaemolyticus and bacteriophages that could form plaques on ten strains of pandemic and nonpandemic v. parahaemolyticus. in addition, v. parahaemolyticus isolates from clinical samples from hat yai hospital over the same period we ... | 2014 | 24682339 |
| design and evaluation of a tandemly arranged outer membrane protein u (ompu) multi-epitope as a potential vaccine antigen against vibrio mimicus in grass carps (ctenopharyngodon idella). | vibrio mimicus (v. mimicus) is an extracellular pathogen that causes ascites disease in aquatic animals. in our previous studies, the outer membrane protein u (ompu) of v. mimicus has been proven to be a protective antigen, and several mimotopes of the protein were identified. here, a tandemly arranged multi-epitope peptide (named 6epis) was designed with six mimotopes and heterologously expressed. then, the immunoprotection efficacy of recombinant 6epis (r6epis) was evaluated in grass carps (ct ... | 2014 | 24751414 |
| isolation and screening of probiotic candidates from marron, cherax cainii (austin, 2002) gastrointestinal tract (git) and commercial probiotic products for the use in marron culture. | six strains of bacteria including bacillus mycoides (a10) and shewanella species (a12) isolated from healthy marron intestine, bacillus species (pm1), bacillus subtilis (pm3), bacillus sp. (pm4) and bacillus sp. (aq) from commercial probiotic products were investigated for probiotic potential in marron culture. antibiotic susceptibility tests indicated pm3 and pm4 were susceptible to all nine antibiotics evaluated. a10, a12 and aq were resistant to class penicillins, whereas pm1 was resistant to ... | 2015 | 24917311 |
| a novel extracellular protease of vibrio mimicus that mediates maturation of an endogenous hemolysin. | vibrio mimicus, a human pathogen that causes gastroenteritis, produces an enterotoxic hemolysin as a virulence factor. the hemolysin is secreted extracellularly as an inactive protoxin and converted to a mature toxin through removal of the n-terminal propeptide, which comprises 151 amino acid residues. in this study, a novel protease having the trypsin-like substrate specificity was purified from the bacterial culture supernatant. the n-terminal amino acid sequence of the purified protein was id ... | 2014 | 25040152 |
| construction of chimeric human epidermal growth factor containing short collagen-binding domain moieties for use as a wound tissue healing agent. | among the various human growth factors, epidermal growth factor (hegf, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. owing to their multiple activities, egfs are used as pharmaceutical and cosmetic agents. however, their low productivity, limited target specificity, and short half-life inhibit their ap ... | 2015 | 25152055 |
| identification of capsule, biofilm, lateral flagellum, and type iv pili in vibrio mimicus strains. | vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. it is distributed worldwide. factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. in pathogenic vibrio species such as v. cholerae, vibrio. parahaemolyticus and vibrio vulnificu ... | 2014 | 25246027 |
| first pediatric case of chromobacterium haemolyticum causing proctocolitis. | bloody diarrhea in children is usually due to either infectious or inflammatory etiology, but infection is far more common than inflammatory bowel disease in children worldwide. if, however, the patient has unfavorable response to antibiotics and a definite infectious agent has yet to be identified; colonoscopy should be performed. the current patient presented with acute onset of mucous bloody diarrhea. stool culture was initially identified as vibrio mimicus and later identified as aeromonas s ... | 2014 | 25252051 |
| the master activator of inca/c conjugative plasmids stimulates genomic islands and multidrug resistance dissemination. | dissemination of antibiotic resistance genes occurs mostly by conjugation, which mediates dna transfer between cells in direct contact. conjugative plasmids of the inca/c incompatibility group have become a substantial threat due to their broad host-range, the extended spectrum of antimicrobial resistance they confer, their prevalence in enteric bacteria and their very efficient spread by conjugation. however, their biology remains largely unexplored. using the inca/c conjugative plasmid pvcr94δ ... | 2014 | 25340549 |
| architecture of the superintegron in vibrio cholerae: identification of core and unique genes. | vibrio cholerae, the etiologic agent of cholera, is indigenous to aquatic environments. the v. cholerae genome consists of two chromosomes; the smallest of these harbors a large gene capture and excision system called the superintegron (si), of ~120 kbp. the flexible nature of the si that results from gene cassette capture, deletion and rearrangement is thought to make it a hotspot of v. cholerae diversity, but beyond the basic structure it is not clear if there is a core genome in the si and if ... | 2013 | 25580216 |
| outer membrane protein u (ompu) mediates adhesion of vibrio mimicus to host cells via two novel n-terminal motifs. | vibrio mimicus (v.mimicus) is a causative agent of ascites disease in aquatic animals. our previous studies have demonstrated that the outer membrane protein u (ompu) from v.mimicus is an immunoprotective antigen with six immunodominant linear b-cell epitopes. although the n-terminus of ompu contains potential binding motifs, it remained unclear whether ompu possesses adhesion function. here, the adhesive capacity of recombinant ompu and v.mimicus to epithelioma papulosum cyprinid (epc) cells wa ... | 2015 | 25742659 |
| defensive effects of human intestinal antimicrobial peptides against infectious diseases caused by vibrio mimicus and v. vulnificus. | of human pathogenic vibrio species, v. mimicus causes gastroenteritis whereas v. vulnificus causes fatal septicemia after consumption of contaminated seafood. these two pathogens produce hemolytic toxins termed v. mimicus hemolysin (vmh) and v. vulnificus hemolysin (vvh), respectively. these toxins elicit the cytolysis of various eukaryotic cells, as well as erythrocytes. the human intestine secretes cationic antimicrobial peptides (amps) to prevent infectious diseases. paneth cells in the small ... | 2014 | 25744216 |
| gbpa as a novel qpcr target for the species-specific detection of vibrio cholerae o1, o139, non-o1/non-o139 in environmental, stool, and historical continuous plankton recorder samples. | the vibrio cholerae n-acetyl glucosamine-binding protein a (gbpa) is a chitin-binding protein involved in v. cholerae attachment to environmental chitin surfaces and human intestinal cells. we previously investigated the distribution and genetic variations of gbpa in a large collection of v. cholerae strains and found that the gene is consistently present and highly conserved in this species. primers and probe were designed from the gbpa sequence of v. cholerae and a new taq-based qpcr protocol ... | 2015 | 25915771 |
| highly diverse recombining populations of vibrio cholerae and vibrio parahaemolyticus in french mediterranean coastal lagoons. | vibrio parahaemolyticus and vibrio cholerae are ubiquitous to estuarine and marine environments. these two species found in mediterranean coastal systems can induce infections in humans. environmental isolates of v. cholerae (n = 109) and v. parahaemolyticus (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a multilocus sequence-based analysis (mlsa). v. cholerae isolates were all ctxa negative and only one isolate of v. parahaemolyti ... | 2015 | 26236294 |
| vibrio spp. from macrobrachium amazonicum prawn farming are inhibited by moringa oleifera extracts. | to investigate the in vitro antimicrobial potential of extracts of stem, leaves, flowers, pods and seeds of moringa oleifera (m. oleifera) against vibrio spp. from hatchery water and the prawn macrobrachium amazonicum. | 2015 | 26614991 |
| developing universal genetic tools for rapid and efficient deletion mutation in vibrio species based on suicide t-vectors carrying a novel counterselectable marker, vmi480. | despite that vibrio spp. have a significant impact on the health of humans and aquatic animals, the molecular basis of their pathogenesis is little known, mainly due to the limited genetic tools for the functional research of genes in vibrio. in some cases, deletion of target dnas in vibrio can be achieved through the use of suicide vectors. however, these strategies are time-consuming and lack universality, and the widely used counterselectable gene sacb does not work well in vibrio cells. in t ... | 2015 | 26641275 |
| molecular and genomic characterization of vibrio mimicus isolated from a frozen shrimp processing facility in mexico. | vibrio mimicus is a gram-negative bacterium responsible for diseases in humans. three strains of v. mimicus identified as v. mimicus 87, v. mimicus 92 and v. mimicus 93 were isolated from a shrimp processing facility in guaymas, sonora, mexico. the strains were analyzed using several molecular techniques and according to the cluster analysis they were different, their similarities ranged between 51.3% and 71.6%. eric-pcr and rapd (vmh390r) were the most discriminatory molecular techniques for th ... | 2016 | 26730584 |
| enterobacteria and vibrio from macrobrachium amazonicum prawn farming in fortaleza, ceará, brazil. | to investigate the isolation of enterobacteria associated with macrobrachium amazonicum (m. amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of vibrio strains. | 2016 | 26851782 |
| regulation of vibrio mimicus metalloprotease (vmp) production by the quorum-sensing master regulatory protein, luxr. | vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. this pathogen secretes a relatively important toxin named v. mimicus metalloprotease (vmp). in this study, we clarified regulation of the vmp production according to the quorum-sensing master regulatory protein named luxr. first, the full length of luxr gene, encoding luxr, was detected in v. mimicus strain e-37, an environmental isolate. next, the putative consensus binding ... | 2016 | 27160384 |
| corrigendum to "immunological responses of customised probiotics-fed marron, cherax tenuimanus, (smith 1912) when challenged with vibrio mimicus" [fish shellfish immunol. 35 (2) (2013) 262-270]. | 2016 | 27475718 | |
| complete genome sequence of vibrio mimicus strain sccf01 with potential application in fish vaccine development. | 2016 | 27763808 | |
| screening a phage display library for two novel ompu-binding peptides with adhesion antagonistic activity against vibrio mimicus. | vibrio mimicus is a pathogen that causes ascites disease in fish. we have previously demonstrated that the outer membrane protein u (ompu) is an important adhesin in v. mimicus. here eight specific ompu-binding phage clones, which presented three different ompu-binding peptides (designated p1, p2, p3), were screened from a commercially available phage displayed 12-mer peptide library using rompu protein as target. then, synthetic ompu-binding peptides were measured for their adhesion antagonisti ... | 2016 | 27832083 |
| identification of three novel b-cell epitopes of vmh protein from vibrio mimicus by screening a phage display peptide library. | vibrio mimicus is the causative agent of ascites disease in fish. the heat-labile hemolytic toxin designated vmh is an immunoprotective antigen of v. mimicus. however, its epitopes have not been well characterized. here, a commercially available phage displayed 12-mer peptide library was used to screen epitopes of vmh protein using polyclonal rabbit anti-rvmh protein antibodies, and then five positive phage clones were identified by sandwich and competitive elisa. sequences analysis showed that ... | 2016 | 27863546 |
| vibrio mimicus as the rare cause of acute diarrheal illness. | vibrio mimicus, although named for having many of the same virulent factors as vibrio cholera, is a rare cause of significant gastrointestinal illness. like many of the vibrio species, the strongest risk factor for v. mimicus infection is seafood consumption. after consuming crabs, a 64-year-old male presented with a three day history of voluminous, non-bloody, water diarrhea. the severity of the diarrhea caused the patient to have orthostatic hypotension and acute kidney injury, which improved ... | 2017 | 28045686 |
| occurrence and virulence properties of vibrio and salinivibrio isolates from tropical lagoons of the southern caribbean sea. | the vibrionaceae are gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. the aim of this research was to investigate the occurrence and virulence properties of vibrio and salinivibrio isolated from lagoons at cuare wildlife refuge and margarita island in the southern caribbean sea. water, plankton and oyster samples were collected during october 2011 and march 2012 and examined by s ... | 2017 | 28324231 |
| in vitro anti-oxidant and anti-microbial potentiality investigation of different fractions of caryota urens leaves. | caryota urens is a member of the arecaceae family and a common plant in the southeast asian region. this plant has been reported as an anti-microbial agent in recent years. thus, we aimed to find out the mic (minimum inhibitory concentration) against different pathogenic microorganism. | 2016 | 28536384 |
| virulence-associated factors in vibrio cholerae non-o1/non-o139 and v. mimicus strains isolated in ornamental fish species. | during recent decades, ornamental fish have proven to be one of the fastest growing categories of pets in europe. in this framework, we evaluated both the potential pathogenic and zoonotic risks caused by 53 vibrio cholerae non-o1/non-o139 and a vibrio mimicus strain isolated from ornamental fish species mostly originating from south-east asia countries between 2000 and 2015 in italy. all the strains were firstly identified at species level by biochemical, phylogenetic and mass spectrometry (mat ... | 2017 | 28677232 |