Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| sugar composition of the polysaccharide portion of lipopolysaccharides of vibrio fluvialis, vibrio vulnificus, and vibrio mimicus. | a chemotaxonomic study was carried out on vibrio fluvialis and v. vulnificus on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharides (lps). a previously developed rapid method of preparing samples for compositional sugar analysis was employed. nineteen o-serogroups of v. fluvialis were divided into 14 chemotypes while seven o-serogroups of v. vulnifucus were divided also into seven chemotypes since the polysaccharide portion of lps of each serogroup has a ... | 1989 | 2482433 |
| [isolation of vibrio mimicus from shallow waters]. | 1989 | 2490717 | |
| isolation of vibrio mimicus from a patient with gastroenteritis in belgium. | 1989 | 2495957 | |
| stress-induced behavioral depression in the rat is associated with a decrease in gaba receptor-mediated chloride ion flux and brain benzodiazepine receptor occupancy. | rats exposed to inescapable tailshock exhibit deficits in learning a simple shuttlebox escape task 24 h later. this syndrome has been termed 'behavioral depression' or 'learned helplessness', and is a model of stress-induced depression. in the present study a significant (25%) decrease in gaba receptor-mediated chloride ion flux as measured by muscimol-stimulated 36cl- uptake in synaptoneurosomes was found in the cerebral cortices of rats that failed the shuttlebox task as compared to naive cont ... | 1989 | 2546650 |
| amoxicillin in the treatment of asymptomatic bacteriuria in pregnancy: a single dose of 3 g amoxicillin versus a 4-day course of 3 doses 750 mg amoxicillin. | a prospective, randomized, controlled comparative clinical trial was carried out with the aim of investigating the efficacy and tolerance of two different dosage regimens of amoxicillin in the treatment of asymptomatic bacteriuria during pregnancy. patients in group a received a single dose of 3 g amoxicillin, which was compared to a 4-day course of 3 x 750 mg amoxicillin tablets taken every 8 h (group b). significant bacteriuria (cfu greater than or equal to 10(5)/ml clean catch midstream urine ... | 1989 | 2659442 |
| heterogeneity of fast-oxidative muscle fibers of chicken demonstrated by anti-myosin monoclonal antibodies. | the fiber type composition of two fast muscles of the chicken, namely, adductor superficialis (as) and pectoralis major (pm) was examined by the histochemical myosin atpase staining and immunochemical techniques using monoclonal antibodies (mcabs). two new mcabs produced against the myosin of the anterior latissimus dorsi (ald) muscle of the chicken and named ald-122 and ald-83 were characterized to be specific for myosin heavy chain (mhc) and for myosin light chain-1 respectively. they were use ... | 1989 | 2737924 |
| ecology of vibrio mimicus in aquatic environments. | an environmental study was done to examine the prevalence of vibrio mimicus in some aquatic environments of dhaka, bangladesh, and of okayama, japan. water samples from dhaka environments and water and plankton samples from okayama environments were quantitatively as well as qualitatively analyzed throughout the seasons for v. mimicus. the organism was isolated from bangladesh environments throughout the year, whereas it was not isolated in okayama when the water temperature fell below 10 degree ... | 1989 | 2782878 |
| a vasopressin and oxytocin containing nucleus in the pig hypothalamus that shows neuronal changes during puberty. | a vasopressin and oxytocin containing nucleus is described for the first time in the pig hypothalamus. it is located near the third ventricle, just dorsal to the suprachiasmatic nucleus, and consists of magnocellular neurons, similar to those of the supraoptic nucleus and paraventricular nucleus. morphometric analysis of neuronal number, size, density, and volume was performed at four different ages: 1 day, 7 weeks, 16 weeks, and 30 weeks postnatally. no sex difference in these parameters was ob ... | 1990 | 2077051 |
| subunit analysis of bovine cytochrome c oxidase by reverse phase high performance liquid chromatography. | bovine cytochrome c oxidase subunits were separated by reverse phase high performance liquid chromatography using a c4 column eluted with water and an acetonitrile gradient, both containing 0.1% trifluoroacetic acid. subunits i and iii precipitated in this solvent and could not be analyzed; the remaining eleven subunits were dissociated, denatured, soluble and could be resolved by elution from the column. the protein subunit eluting in each chromatographic peak was identified by a combination of ... | 1990 | 2168156 |
| purification and characterization of a protease produced by vibrio mimicus. | a protease produced by vibrio mimicus was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on sephacryl s-100 and mono q monobeads. the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) of the final preparation of the enzyme revealed the homogeneity of the purified enzyme. conventional page showed that the purified protease migrated as a single band with protease activity. the molecular weight of the protease was estimated to ... | 1990 | 2254038 |
| nucleotide sequence of the thermostable direct hemolysin gene (tdh gene) of vibrio mimicus and its evolutionary relationship with the tdh genes of vibrio parahaemolyticus. | the gene encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus was previously cloned from the chromosome of vibrio mimicus. the nucleotide sequence of the hemolysin gene was determined in this study. the gene proved to be a variant of the thermostable direct hemolysin gene (tdh gene) and was designated as vm-tdh because the sequence divergences between the vm-tdh gene and four tdh genes of v. parahaemolyticus were 2.1-3.0%, while the sequence divergen ... | 1990 | 2272498 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| production of a monoclonal antibody to vibrio cholerae non-o1 heat-stable enterotoxin (st) which is cross-reactive with yersinia enterocolitica st. | a monoclonal antibody (mab) against synthetic heat-stable enterotoxin of vibrio cholerae non-o1 (nag-st) was produced. the mab, namely, 2f, belonged to the immunoglobulin g1 class. ascitic fluid drawn from pristane-primed balb/c mice injected with a 2f-producing clone demonstrated anti-nag-st activity which could be detected in enzyme-linked immunosorbent assay even at a dilution of 1:128,000. fifty-fold-diluted ascitic fluid could completely neutralize the activity of nag-st (synthetic and nati ... | 1990 | 1696928 |
| variation in epitopes of the b subunit of vibrio cholerae non-o1 and vibrio mimicus cholera toxins. | monoclonal antibodies reacting with the b subunit of vibrio cholerae o1 strain 569b cholera toxin (ct-b) were used to identify unique and common epitopes of v. cholerae non-o1 and vibrio mimicus ct-b. vibrio cholerae non-o1 strains produced ct-b showing three monoclonal antibody reaction patterns (epitypes), which corresponded with epitypes described previously for v. cholerae o1 classical biotype ct-b (ct1), el tor biotype ct-b (ct2), and a unique v. cholerae non-o1 ct-b (ct3), which lacked an ... | 1990 | 1697780 |
| ecology of vibrio mimicus in aquatic environments. | [this corrects the article on p. 2077 in vol. 55.]. | 1990 | 16348134 |
| erratum: variation in epitopes of the b subunit of vibrio cholerae non-o1 and vibrio mimicus cholera toxins. | 1990 | 22049941 | |
| role of vibrio mimicus protease in enterotoxigenicity. | recently, we have purified and characterised a metalloprotease produced by vibrio mimicus. the role of v. mimicus protease (vmp) in fluid accumulation (fa) in rabbit ileal loops (ril) was investigated. although live cells or crude vmp prepared from cell-free culture supernatant showed activity in ril, the purified vmp failed to induce fa in the ril. when vmp in crude preparation was inactivated by preparing the fraction in the presence of egta (ethyleneglycol-bis [beta-aminoethylether]-n, n, n1, ... | 1991 | 1800566 |
| purification and characterization of a hemolysin of vibrio mimicus that relates to the thermostable direct hemolysin of vibrio parahaemolyticus. | a hemolysin (designated vm-rtdh) from vibrio mimicus (aq0915-e13) was purified by ammonium sulfate fractionation and successive column chromatography with deae-sephadex a-25, hydroxyapatite, mono q, superose 12 and phenyl-superose. the mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. the isoelectric point of vm-rtdh was approximately ph 4.9. the hemolytic activity of vm-rtdh was stable upon heating at 100 degrees c for 10 min, similar to that ... | 1991 | 1804756 |
| vascular permeability enhancement by vibrio mimicus protease and the mechanisms of action. | vibrio mimicus, a causative agent of gastroenteritis, has also been reported to attribute to extraintestinal infections. recently we have purified a metalloprotease produced by the pathogen: however, the role of the protease in v. mimicus infection has not been documented. the v. mimicus protease (vmp) was found to enhance vascular permeability and form edema when injected into the dorsal skin of guinea pig and rat. the permeability enhancement by vmp was observed in a dose-dependent manner in b ... | 1991 | 1808459 |
| structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6. | we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ... | 1991 | 1828805 |
| expression of antifreeze proteins in transgenic plants. | the quality of frozen fruits and vegetables can be compromised by the damaging effects of ice crystal growth within the frozen tissue. antifreeze proteins in the blood of some polar fishes have been shown to inhibit ice recrystallization at low concentrations. in order to determine whether expression of genes of this type confers improved freezing properties to plant tissue, we have produced transgenic tobacco and tomato plants which express genes encoding antifreeze proteins. the afa3 antifreez ... | 1991 | 1932678 |
| purification and characterization of a heat-stable enterotoxin of vibrio mimicus. | a heat-stable enterotoxin produced by vibrio mimicus (vm-st) was studied. vm-st was purified from a culture supernatant of v. mimicus strain aq-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both sp-sephadex c-50 and deae-sephadex a-25, and hplc, and the recovery rate was about 15%. purified vm-st was heat-stable. vm-st activity was cross-neutralized by anti-sth antiserum. the amino acid composition of the purified vm-st was determi ... | 1991 | 2044934 |
| [surveys on the contamination of marine fish with non-o1 vibrio cholerae and vibrio mimicus and food poisoning cases by these organisms]. | the present paper describes the relationship between the contamination with non-o1 vibrio cholerae and vibrio mimicus of marine fish, with special reference to the seasonal variation and the concentration of contamination, and the actual cases of domestic food poisoning by these organisms. a 10 year survey revealed that non-o1 vibrio cholerae (non-o1 v. cholerae) strains were frequently isolated from fish during the summer season with some variations from one year to another, and isolates from f ... | 1991 | 2066602 |
| evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among vibrio species. | the tdh gene of vibrio parahaemolyticus which encodes the thermostable direct hemolysin has been found in some strains of other vibrio species. analysis of seven tdh genes cloned from v. parahaemolyticus, vibrio mimicus, and non-o1 vibrio cholerae revealed that all tdh genes were flanked by insertion sequence-like elements (collectively named isvs) or related sequences derived from genetic rearrangement of isvs. the isvs possessed 18-bp terminal inverted repeats highly homologous to those of is9 ... | 1991 | 1650342 |
| production of hemagglutinins and pili by vibrio mimicus and its adherence to human and rabbit small intestines in vitro. | clinical isolate of vibrio mimicus were examined for production of cell-associated hemagglutinin (ha) and pili and for adherence to formalin-fixed human intestinal mucosa. v. mimicus grown on cfa agar for 3 h at 37 degrees c possessed ha and adhered better to the mucus layer than to the epithelial cell surface. a significant correlation was found between the ha titers and adherence ability to the epithelial cell surface of villi (p less than 0.05); adherence to the ileal lymphoid follicle-associ ... | 1992 | 1349543 |
| lipopolysaccharide composition and virulence properties of clinical and environmental strains of vibrio fluvialis and vibrio mimicus. | vibrio mimicus strains w-26768 (stool isolate) and n-1301 (environmental isolate) and vibrio fluvialis strains aa-18239 (stool isolate) and m-940 (environmental isolate) were studied for virulence properties and lipopolysaccharide composition. all four strains were hydrophobic, produced cytotoxin, adhered to hela cells and showed mannose-sensitive agglutination of guinea pig erythrocyte. the strains were negative for enterotoxin production and were mostly susceptible to the common antibiotics. t ... | 1992 | 1383675 |
| [enteropathogenicity and enteropathogenic toxin production of vibrio mimicus]. | enteropathogenicity and enteroreactive-toxins were examined in 66 strains of vibrio mimicus and the following results were obtained. frequencies of enteropathogenic strains judged by the result of suckling mouse tests were 11/13 (85%) for clinical isolates and 37/53 (70%) for fish or environmental isolates. frequencies of preservation of cholera toxin gene and nag-st gene were 2 and 15%, respectively, for 48 enteropathogenic strains, and 0 and 6%, respectively, for 18 non-enteropathogenic strain ... | 1992 | 1402070 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| the flexor reflex mediated by group ii afferent fibers: effects of morphine-hcl and mephenesin. | the effects of morphine-hcl and mephenesin on the flexor reflex mediated by group ii afferent fibers were investigated. the flexor reflex was recorded by means of the electromyogram (emg) evoked in the muscle tibialis anterior by stimulation of the ipsilateral tibial nerve in urethane-alpha-chloralose anesthetized rats. afferent volleys corresponding to the phasic emg component of the flexor reflex with 7.6-msec latency (flexor emg: femg) were also recorded using the double volley technique. the ... | 1992 | 1479741 |
| the serological response of the common seal (phoca vitulina) and the grey seal (halichoerus grypus) to phocine distemper virus as measured by a canine distemper virus neutralisation test. | the application of a canine distemper virus (cdv) neutralisation test revealed that antibody titres of up to 11,620 were present in the sera of common seals during the epizootic of phocine distemper in british waters. however, during the later stages of the epizootic and afterwards, a surprisingly high proportion of animals were still seronegative. titres of from 90 to 183 were common in diseased animals and were probably indicative of partial immunosuppression. a few animals with proven phocine ... | 1992 | 1594940 |
| vibrio mimicus bacteraemia in a child. | 1992 | 1619242 | |
| total hepatic mesh wrap for hemostasis. | to improve hemostasis and avoid the complications of perihepatic packing, a technique of hepatic tamponade using total mesh wrap is reported. the method is geometrically, technically and mechanically feasible and appears to be effective in controlling severe parenchymatous bleeding. | 1992 | 1636144 |
| evaluation of virulence factors in environmental isolates of vibrio species. | strains of vibrio parahaemolyticus, vibrio fluvialis and vibrio mimicus isolated from seafood and seawater were examined for characteristics related to infectivity, such as enzymatic activity and animal assays. all strains hydrolysed dna, starch, gelatin and chitin. variable results were obtained with the haemolysin, chondroitin, collagen, elastin and lecithin tests. production of thermostable direct haemolysin by v. parahaemolyticus was detected in 7.1% strains derived from seafood and 2% from ... | 1993 | 8139466 |
| [care of the injured in the hospital. medico-legal documents furnished them. transmitting care information]. | 1993 | 262925 | |
| vibrio mimicus. | 1993 | 8294347 | |
| acute diarrhoea caused by vibrio mimicus in calcutta. | out of 539 acute diarrhoea cases studied, vibrio mimicus was isolated as a sole pathogen in the faeces of 7 (1.3%) cases. the chief clinical presentations of the seven cases were watery diarrhoea and vomiting. bloody diarrhoea was observed in 2 (28.5%), abdominal pain in 2 (28.57%) and fever in one (14.29) cases. all cases could be effectively treated with ors except 3 (42.85%) cases who required iv ringer's lactate. all v. mimicus strains isolated in the study were uniformly susceptible to tetr ... | 1993 | 8294349 |
| structural studies of the vibrio mimicus w-26768 o-antigen polysaccharide. | the structure of the vibrio mimicus w-26768 o-antigen polysaccharide has been investigated by sugar and methylation analyses, smith degradation, and nmr spectroscopy. it is proposed that it is composed of chains of beta-(1-->4)-linked 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucopyranosyl residues (d-qui(p)3nr), approximately 60% of which are substituted in the 2-position with 2-acetamido-2-deoxy-alpha-d-galactopyranosyl groups. the polysaccharide does not seem to be composed of oligosaccharid ... | 1993 | 8324759 |
| high prevalence of thermostable direct hemolysin (tdh)-like toxin in vibrio mimicus strains isolated from diarrhoeal patients. | a total of 17 isolates of vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. sixteen (94%) clinical isolates and one (50%) from food produced tdh-like toxin, whereas none of the environmental isolates did so. the food from which v. mimicus with tdh-like toxin production was isolated, was one which had caused food poisoning. only one environmental strain produced ct-like toxin, whilst st-like toxin was not detected from any strains tested. | 1993 | 8348932 |
| studies on hemolytic action of a hemolysin produced by vibrio mimicus. | some properties and mechanism of action of a hemolysin (vmh) produced by an enteropathogenic vibrio mimicus strain was examined. vmh was heat-labile and inhibited by addition of divalent cations, including ca2+, mg2+ and mn2+. the hemolysis by vmh was inhibited by incubating with gangliosides, suggesting that the ganglioside was the binding site on the erythrocyte membrane for vmh. existence of a galactose moiety on reducing end of the ganglioside molecule and a sialic acid on the galactose moie ... | 1993 | 8355623 |
| phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences. | the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ... | 1993 | 8427811 |
| development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene. | an alkaline phosphatase-conjugated 30-mer oligonucleotide probe was developed to detect the cholera toxin gene (ctx) in vibrio cholerae o1. for rapid identification, v. cholerae o1 was grown on selective agar (thiosulfate-citrate-bile salts agar) or in alkaline peptone water and organisms were transferred directly to nylon membranes. lysis of cells, denaturation of dna, neutralization, and hybridization were carried out on the membrane. these procedures required only 3 h for completion. the resu ... | 1993 | 8501233 |
| fatal gastroenteritis due to vibrio fluvialis and nonfatal bacteremia due to vibrio mimicus: unusual vibrio infections in two patients. | 1994 | 7811875 | |
| existence of a novel hemagglutinin having no protease activity in vibrio mimicus. | the protease elaborated by vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known ha/protease. a non-protease hemagglutinin (ha) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of v. mimicus. this ha (v. mimicus ha: vmha) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocy ... | 1994 | 7968677 |
| identification of the siderophores from vibrio hollisae and vibrio mimicus as aerobactin. | hydroxamate siderophores were purified from low-iron cultures of vibrio hollisae atcc 33564 and vibrio mimicus atcc 33653. by a combination of 1h and 13c nmr spectroscopy, fast atom bombardment mass spectrometry, and compositional analysis, both of the siderophores were identified as aerobactin, a citrate-based dihydroxamate siderophore, which is highly prevalent in species of the family enterobacteriaceae. four other clinical strains belonging to these species also produced aerobactin. in respo ... | 1994 | 8013878 |
| a gene for the enterotoxin zonula occludens toxin is present in vibrio mimicus and vibrio cholerae o139. | the presence of the zonula occludens toxin (zot) gene, which encodes an enterotoxin produced by serotype o1 strains of the pathogenic bacterium, vibrio cholerae, in addition to cholera toxin, was investigated in selected strains of v. mimicus and the new pandemic v. cholerae non-o1 serotype o139. the zot gene was detected by polymerase chain reaction (pcr) amplification, using sets of primers based on the sequence of the v. cholerae o1 zot sequence. pcr amplification of genomic dnas of both chol ... | 1994 | 8050719 |
| an analysis of the v1 and v2 regions of vibrio cholerae and vibrio mimicus 16s rrna. | the v1 and v2 variable regions of the 16s rrna gene of three strains of v. cholerae and one strain of v. mimicus were amplified by pcr. fragments containing both regions were cloned into m13mp18 using smal and sequenced by the dideoxy method. the 263-bp sequence from a strain isolated during the 1991 cholera outbreak in brazil was deposited in genbank under the accession number l05178. except for an extra g in one of the strains, the three v. cholerae sequences were identical. the v. mimicus seq ... | 1994 | 8090995 |
| vibrio mimicus with multiple toxin types isolated from human and environmental sources. | a collection of 13 strains of vibrio mimicus, including both clinical and environmental isolates from different geographic regions, was examined for various toxins. one strain of environmental origin produced cholera-like toxin (ct) which was completely absorbed with anti-ct immunoglobulin g, five strains produced a haemolysin that cross-reacted with the thermostable direct haemolysin of v. parahaemolyticus and dna from two strains hybridised with a dna probe specific for the heat-stable enterot ... | 1994 | 8114069 |
| nucleotide sequence of a novel arylesterase gene from vibro mimicus and characterization of the enzyme expressed in escherichia coli. | a gene coding for an arylesterase of vibrio mimicus was cloned. sequence determination reveals that the esterase gene has an open reading frame of 600 nucleotides which encodes a protein of m(r) 22,300. the deduced amino acid sequence contain a pentapeptide gdsls (residues 27-31), which was also found in the phospholipid-cholesterol acyltransferase from aeromonas hydrophila. substitution of ser-29 by alanine or cysteine in the cloned gene abolished the esterase activity in the tributyrin plate a ... | 1994 | 8141782 |
| vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus vibrio. | using a 0.27 kb dna probe specific for the heat-stable enterotoxin gene (nag-st) of vibrio cholerae non-o1, 1109 strains representing 17 species of the genus vibrio, isolated from clinical and environmental sources were examined. the nag-st gene was preponderantly associated with strains classified as v. mimicus; 16.8% of these strains hybridized. it was more frequent in the clinical isolates (22.6%) than in the environmental isolates (13.7%). the incidence of nag-st gene-positive strains of v. ... | 1994 | 24420888 |
| cofactor recycling in liquid membrane-enzyme systems. | 1995 | 177010 | |
| iron transport genes of the pjm1-mediated iron uptake system of vibrio anguillarum are included in a transposonlike structure. | the pjm1 genes encoding the proteins involved in iron transport in the anguibactin iron uptake system were found to be flanked by insertion sequences in a composite transposonlike structure. these vibrio anguillarum insertion sequences, isv-a1 and isv-a2, are related to is903, is102, and the isvs found in vibrio parahaemoliticus, vibrio mimicus, and non-o1 vibrio cholerae flanking various tdh (thermostable direct hemolysin) genes. the inverted repeats at the ends of isv-a1 and isv-a2 have no mor ... | 1995 | 7568465 |
| [in vitro antibacterial activity of balofloxacin (blfx) against isolates from patients with bacterial enteritis]. | in vitro antibacterial activity of balofloxacin (blfx), a newly developed fluoroquinoline, was compared with that of norfloxacin (nflx), ofloxacin (oflx) and ciplofloxacin (cpfx). bacterial strains used in this experiment were freshly isolated from patients with infectious enteritis just before blfx therapy. the isolates were 43 strains of vibrio cholerae o1, 1 strain of campylobacter sp., 4 strains of aeromonas spp., 3 strains of plesiomonas shigelloides, 1 strain of vibrio mimicus and 1 strain ... | 1995 | 7594800 |
| vibrio mimicus arylesterase has thioesterase and chymotrypsin-like activity. | a vibrio mimicus serine arylesterase and an escherichia coli thioesterase/serine protease share 49.4% amino acid identity. the arylesterase has thioesterase activity for benzoyl-coa and chymotrypsin-like activity for n-carbobenzoxy-l-phenylalanine p-nitrophenyl ester (nbpnpe). the gene encoding the v. mimicus enzyme is designated etpa. substituting ser31 of the v. mimicus enzyme with a glycine or an alanine altered its activity. in comparison with wild type enzyme, the s31a enzyme showed a 5-fol ... | 1995 | 7646502 |
| the sixth and seventh cholera pandemics are due to independent clones separately derived from environmental, nontoxigenic, non-o1 vibrio cholerae. | the dna sequences of the asd genes from 45 isolates of vibrio cholerae (19 clinical o1 isolates, 2 environmental nontoxigenic o1 isolates, and 24 isolates with different non-o1 antigens) were determined. no differences were found within either sixth- or seventh-pandemic isolates; however, variation was found between the two forms and among the non-o1 isolates. o139 isolates had sequences identical to those of seventh-pandemic isolates. phylogenetic trees with vibrio mimicus as the outgroup sugge ... | 1995 | 7768818 |
| crystallization and preliminary x-ray crystallographic analysis of arylesterase from vibrio mimicus. | single crystals of arylesterase (ec 3.1.1.2) from vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. the present crystals diffract up to 2.2 a resolution and belong to monoclinic space group p2(1). the cell dimensions are a = 55.65(1) a, b = 53.46(1) a, c = 65.79(1) a, and beta = 106.54(1) degrees. there are two molecules of molecular weight 22 kda in an asymmetric unit with a solvent content of 43%. | 1995 | 8528091 |
| site-directed mutagenesis of a novel serine arylesterase from vibrio mimicus identifies residues essential for catalysis. | site-directed mutagenesis (sdm) of an arylesterase (the arylesterase) from vibrio mimicus revealed that residues s29, h153, and d96 constituted a catalytic triad. the use of a serine residue for ester hydrolysis by the arylesterase proves that the enzyme is a novel serine arylesterase. sdm also showed that d28 was necessary for the esterase activity; to our knowledge it is the first time that a residue immediately preceding the active-site serine in esterases was shown biochemically to possess s ... | 1996 | 8619880 |
| expression of virulence-related properties by, and intestinal adhesiveness of, vibrio mimicus strains isolated from aquatic environments. | a study of the major pathogenic characteristics of vibrio mimicus was carried out with 77 strains isolated from aquatic environments in okayama, japan. of the strains tested, 96% demonstrated in vitro adherence to the rabbit intestinal mucosa, of which 36, 20, and 43% belonged to the strongly, moderately, and weakly adhesive groups, respectively. of the 27 strains which appeared to be enterotoxigenic in the experiments using rabbit ileal loops, 74% belonged to the strongly adhesive group. all st ... | 1996 | 8837444 |
| vibrio mimicus diarrhea following ingestion of raw turtle eggs. | clinical and epidemiological characteristics of diarrhea associated with vibrio mimicus were identified in 33 hospitalized patients referred to the costa rican national diagnostic laboratory network between 1991 and 1994. the relevant symptoms presented by patients included abundant watery diarrhea, vomiting, and severe dehydration that required intravenous dhaka solution in 83% of patients but not fever. seroconversion against v. mimicus was demonstrated in four patients, from whom acute- and c ... | 1996 | 8919774 |
| purification and characterization of novel hemagglutinins from vibrio mimicus: a 39-kilodalton major outer membrane protein and lipopolysaccharide. | two hemagglutinins (has) mediating the agglutinability to rabbit erythrocytes were isolated from 32-h culture supernatant of enterotoxigenic strain e-33 of vibrio mimicus by ultrafiltration followed by gel filtration and anion-exchange column chromatography. the has were designated r-ha and c-ha on the basis of specific hemagglutinating activity towards rabbit erythrocytes only (r-ha) and towards chicken and rabbit erythrocytes (c-ha). sodium dodecyl sulfate-polyacrylamide gel electrophoresis an ... | 1996 | 8926065 |
| toxigenicity & drug sensitivity of vibrio mimicus isolated from patients with diarrhoea. | in a total of 720 faecal specimens from patients with secretory diarrhoea, vomiting, dehydration, gastroenteritis, cholera and cholera like illnesses, 18 strains of v. mimicus were isolated as pure culture. these were characterized for various toxin types and virulence factors using conventional in vitro and in vivo assays. labile and stable toxins were elaborated by 15 and 2 strains respectively by ligated rabbit ileal loop (ril) and suckling mouse assays. while 15 of the whole cell culture ela ... | 1996 | 8996933 |
| analysis of the structural gene encoding a hemolysin in vibrio mimicus. | an environmental isolate of v. mimicus, strain e-33, has been reported to produce and secrete a hemolysin of 63 kda. the hemolysin is enterotoxic in test animals. the nucleotide sequence of the structural gene of the hemolysin was determined. we found a 2,232 bp open reading frame, which codes a peptide of 744 amino acids, with a calculated molecular weight of 83,903 da. the sequence for the structural gene was closely related to the v. cholerae el tor hlya gene, coding an exocellular hemolysin. ... | 1997 | 9087960 |
| purification and characterization of a hemolysin produced by vibrio mimicus. | vibrio mimicus is a causative agent of human gastroenteritis. this pathogen secretes a pore-forming toxin, v. mimicus hemolysin (vmh), which causes hemolysis by three sequential steps: binding to an erythrocyte membrane, formation of a transmembrane pore, and disruption of the cell membrane. vmh with a molecular mass of 63 kda was purified by ammonium sulfate precipitation and column chromatography with phenyl sepharose hp and superose 6 hr. the hemolytic reaction induced by vmh continued up to ... | 1997 | 9125568 |
| nucleotide sequence of the vmha gene encoding hemolysin from vibrio mimicus. | the structural gene (vmha) of hemolysin from vibrio mimicus (atcc33653) was cloned and sequenced. the vmha gene contains an open reading frame consisting of 2232 nucleotides which can code for a protein of 744 amino acids with a predicted molecular mass of 83,059. the similarity of amino acid sequence shows 81.6% identity with vibrio cholerae el tor hemolysin. | 1997 | 9128176 |
| vibrio mimicus attaches to the intestinal mucosa by outer membrane hemagglutinins specific to polypeptide moieties of glycoproteins. | vibrio mimicus is the closest organism to vibrio cholerae. v. mimicus e-33, which is a highly adhesive and enteropathogenic strain, is known to produce three types of hemagglutinins (has), i.e., a 31-kda exocellular metalloprotease (vm-ha/protease), lipopolysaccharide (vm-lpsha), and a 39-kda major outer membrane protein (vm-ompha). hemagglutination induced by vm-lpsha and vm-ompha was inhibited by glycoproteins, including mucin, fetuin, and asialofetuin, but not by monosaccharides, disaccharide ... | 1997 | 9284134 |
| [distribution of serogroups of vibrio cholerae non-o1 non-o139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups]. | a total of 1898 strains of vibrio cholerae non-o1 non-o139, which had been collected worldwide for the past 3 year period of 1994-1996, were serogrouped. the strains were also examined for presence of cholera toxin (ct) gene (ctx) and nag-st gene, and strains which carried to ctx were further analyzed for their ability to produce ct. in addition, attempts were made to establish novel serogroups for those serologically untypable strains. of those examined, 1,774 strains of v. cholerae non-o1 non- ... | 1997 | 9394556 |
| high prevalence of vibrio cholerae non-o1 carrying heat-stable-enterotoxin-encoding genes among vibrio isolates from a temperate-climate river basin of central italy. | vibrio spp. of clinical interest from the arno river basin (tuscany, italy) were investigated in this study. vibrios were isolated from 70% of water samples. vibrio cholerae non-o1 was the most prevalent species (82% of isolates), followed by vibrio mimicus (10%) and vibrio metschnikovii (8%). recovery of vibrios was correlated with temperature, ph, and various indicators of municipal pollution. none of the 150 vibrio isolates carried ctx-related genomic sequences, whereas 18 (14.6%) of the 123 ... | 1997 | 16535661 |
| estrogen binding and estrogen receptors in the prostate. | 1997 | 175521 | |
| value of transesophageal color doppler echocardiography for detection of different types of atrial septal defect in adults. | in 121 adults, the value of transthoracic and transesophageal color doppler echocardiography for detection of different types of atrial septal defect (asd) or of partial anomalous pulmonary venous return was analyzed. the 121 patients had a total of 129 defects with left-to-right atrial shunting (including eight patients with two types of defects). all of six cases with primum-type asd were diagnosed correctly by both echocardiographic methods. ninety-seven patients showed a secundum-type asd du ... | 1997 | 1389216 |
| [distribution and characteristics of poliovirus strains circulating in poland in 1968-1972. ii. genetic characteristics of strains isolated during the 1968 poliomyelitis epidemic, from sporadic cases and from sewage]. | 1997 | 175225 | |
| structural analysis of the o-antigenic polysaccharide from vibrio mimicus n-1990. | the o-antigenic polysaccharide part of the lipopolysaccharide from vibrio mimicus n-1990 has been investigated. sugar and methylation analysis of native and dephosphorylated polysaccharide together with nmr spectroscopy show that the polysaccharide is composed of tetrasaccharide repeating units. the structure of the repeating unit of the polysaccharide from v. mimicus n-1990 could be determined as: -->4)-alpha-d-galpnac-(1-->3)-beta-d-galpnac-(1-->2)-4,6-p-beta-d-galp-( 1-->3)-alpha-d-galpnac-(1 ... | 1998 | 9490076 |
| isolation and sequence analysis of metalloprotease gene from vibrio mimicus. | the vmc gene encoding a metalloprotease of vibrio mimicus (atcc 33653) was cloned in escherichia coli and sequenced. the vmc gene contained 1884 nt sequence which codes a polypeptide of 628 amino acids with a predicted molecular mass of 71,275 da. the deduced amino acid sequence had the similarity of 68.5% with v. parahaemolyticus metalloprotease. the consensus sequence of a zinc binding motif (hexxh) was identified to be heyth. the zymography analysis showed a gelatinolytic protein band around ... | 1998 | 9602021 |
| cloning and identification of a phospholipase gene from vibrio mimicus. | the phospholipase gene phl was identified from vibrio mimicus (atcc33653) and sequenced. the entire open reading frame (orf) was composed of 1410 nucleotides and encoding 470 amino acids. the phl was placed upstream of hemolysin gene (vmha) with opposite direction of transcription. from the blast search program, the deduced amino acids sequence showed 74.4% identity with phospholipase gene (lec) from v. cholerae el tor. the entire orf of phospholipase gene was amplified by pcr and inserted into ... | 1998 | 9767128 |
| optimization of excimer-forming two-probe nucleic acid hybridization method with pyrene as a fluorophore. | a previously presented homogeneous assay method, named the excimer-forming two-probe nucleic acid hybridization (etph) method, is based on specific excimer formation between two pyrenes attached at the neighboring terminals of two sequential probe oligonucleotides complementary to a single target. in this study, we investigated assay conditions and optimal molecular design of probes for intense excimer emission using a pyrenemethyliodoacetamide-introduced 16mer probe, a pyrene butanoic acid-intr ... | 1998 | 9826766 |
| fetal heart rate during a maternal grand mal epileptic seizure. | although maternal ingestion of antiepileptic drugs is strongly suspected of causing congenital defects, particularly oral clefts, the effect of epilepsy itself or a combined effect of drug intake and epilepsy have not been excluded as etiological factors. very little is known about fetal oxygenation during a maternal grand mal epileptic seizure. we describe two cases in which fetal heart rate was recorded during a maternal epileptic seizure during labor. the first fetus became clearly asphyctic ... | 1998 | 106102 |
| [intramural pseudo-diverticulosis of the oesophagus]. | 1998 | 98947 | |
| comparison of specificity of agonist and antagonist radioligand binding to beta adrenergic receptors. | 1998 | 19707 | |
| [disturbances in phosphate metabolism during parenteral alimentation. hypophosphatemic syndrome]. | 1998 | 24396 | |
| detection of genes encoding cholera toxin (ct), zonula occludens toxin (zot), accessory cholera enterotoxin (ace) and heat-stable enterotoxin (st) in vibrio mimicus clinical strains. | a total of 51 clinical strains of vibrio mimicus were searched for the presence of virulence-associated genes, like ctx, zot or ace genes which locate in "cholera virulence cassette," and the st gene by polymerase chain reaction. moreover, the pathological potential of each clinical strain was also examined by rabbit ileal loop (ril). three strains showed to have the ctx gene, of which only one strain was zot gene-positive. meanwhile, one other strain was zot+ but ctx-. all of these four strains ... | 1998 | 10037216 |
| analysis of 16s-23s rrna intergenic spacer regions of vibrio cholerae and vibrio mimicus. | vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related vibrio mimicus. the two species share many genes coding for proteins, such as ctxab, and show almost identical 16s dna coding for rrna (rdna) sequences. primers targeting conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rdnas were used to amplify the 16s-23s rrna intergenic spacer regions of v. cholerae and v. mimicus. two major (c ... | 1999 | 10224020 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| cross-reaction between a strain of vibrio mimicus and v. cholerae o139 bengal. | of 200 isolates of vibrio mimicus screened, one from water (n-57) agglutinated with v. cholerae o139 polyclonal antiserum (absorbed with a rough strain of v. cholerae only) and not with o139 polyclonal diagnostic antiserum (absorbed with the rough strain and v. cholerae o22 and o155). the antigenic relationship between v. cholerae 0139 and n-57 is of a,b-a,c type, where a is the common antigenic epitope and b and c are unique epitopes. strain n-57 was assigned to a new serogroup of v. cholerae o ... | 1999 | 10482300 |
| lysogenic conversion of environmental vibrio mimicus strains by ctxphi. | the filamentous bacteriophage ctxphi, which encodes cholera toxin (ct) in toxigenic vibrio cholerae, is known to propagate by infecting susceptible strains of v. cholerae by using the toxin coregulated pilus (tcp) as its receptor and thereby causing the origination of new strains of toxigenic v. cholerae from nontoxigenic progenitors. besides v. cholerae, vibrio mimicus strains which are normally tcp negative have also been shown to occasionally produce ct and cause diarrhea in humans. we analyz ... | 1999 | 10531221 |
| [retinal neovascularization: topological, morphological and hemodynamic features (author's transl)]. | 1999 | 91317 | |
| the mesencephalic exterolateral posterior nucleus of the mormyrid fish bryenomyrus niger: efferent connections studied by the hrp method. | 1999 | 91414 | |
| tetralogy of fallot in adults. | 1999 | 91440 | |
| chido, rodgers and c4. in vivo and in vitro coating of red blood cells, grouping and antibody detection. | c4 sucrose/low ionic strength (lis)-coated red blood cells (rbc) are excellent for the detection of the previously 'nebulous' antibodies, anti-chido and anti-rodgers, as well as for serum/plasma typing of these antigens by an inhibition technique. by enzyme treatment of such cells, it is confirmed that the ch and rg antigens reside on the c4d part of the c4 molecule. freshly taken rbc from normal individuals were examined with a sensitive auto-analyzer technique with anti-chido, anti-c4 and anti ... | 1999 | 91269 |
| [evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy]. | 1999 | 88669 | |
| the relationship between intracellular and extracellular potassium in normal and malnourished subjects as studied in leukocytes. | leukocyte potassium has been measured in normal subjects and in malnourished children with and without edema both on admission and during recovery. in nonedematous malnourished children the potassium content was low (328 mmol/kg dry solids) and took 2-3 weeks of rehabilitation to return to normal (374 mmol/kg dry solids). leukocytes from edematous children had normal intracellular potassium values. however, 5 days on a maintenance diet reduced the intracellular potassium in the children with kwa ... | 1999 | 99717 |
| sources of vibrio mimicus contamination of turtle eggs. | vibrio mimicus contamination of sand increased significantly during the arrival of the olive ridley sea turtles (lepidochelys olivacea) at ostional anidation beach, costa rica. statistical analysis supports that eggs are contaminated with v. mimicus by contact with the sand nest. v. mimicus was isolated from eggs of all nests tested, and ctxa+ strains were found in 31% of the nests, all of which were near the estuary. | 1999 | 9872804 |
| cure of burkitt's lymphoma. ten-year follow-up of 157 ugandan patients. | 192 ugandan patients with burkitt's lymphoma were treated with various regimens containing high-dose cyclophosphamide. 21 patients died during induction therapy, and 14 died after partial responses. of 157 patients who responded completely to treatment, 16 were lost to follow-up (10 in 69 died and 72 (51%) are alive and disease-free. 31 of the long-term survivors have relapsed at least once and have been successfully retreated. overall, 34 surviving patients had central-nervous-system involvemen ... | 1999 | 91027 |
| sodium iron nafeedta as an iron fortification compound in central america. absorption studies. | studies were performed in seven children and 98 adults to compare the proportion of iron absorbed when administered as ferric sulfate (fe2(so4)3), nafeedta, hemoglobin (hb), and ferrous ascorbate. studies in children (mostly iron deficient) showed that when the compounds were given with a milk-rice-sugar formula totalling 5 mg fe, iron from hemoglobin was absorbed best, followed by nafeedta and by fe2(so4)3 (mean percent absorption +/-sd = 34.5 +/- 1.5, 8.6 +/- 1.9 and 3.3 +/- 1.5, respectively) ... | 1999 | 96688 |
| mycosis fungoides with verrucous lesions. | 1999 | 84478 | |
| early detection of cancer of oesophagus along caspian littoral. report of a pilot project. | a simple mass-screening procedure for the early detection by brush cytology of oesophageal cancer in 280 adults in high-incidence villages of the caspian littoral is described. the presence of atypical cells in 7%, and the discovery of frank carcinoma in 3 symptom-free people encourages continuation of the project. | 2000 | 87555 |
| reversal of florid diabetic retinopathy. | 2000 | 91797 | |
| steroids in asthma. | 2000 | 91910 | |
| infectious ctxphi and the vibrio pathogenicity island prophage in vibrio mimicus: evidence for recent horizontal transfer between v. mimicus and v. cholerae. | vibrio mimicus differs from vibrio cholerae in a number of genotypic and phenotypic traits but like v. cholerae can give rise to diarrheal disease. we examined clinical isolates of v. mimicus for the presence of ctxphi, the lysogenic filamentous bacteriophage that carries the cholera toxin genes in epidemic v. cholerae strains. four v. mimicus isolates were found to contain complete copies of ctxphi. southern blot analyses revealed that v. mimicus strain pt5 contains two ctx prophages integrated ... | 2000 | 10678967 |
| characterization of an arylesterase from lactobacillus helveticus cnrz32. | an esterase gene (esta) was isolated from a previously constructed genomic library of lactobacillus helveticus cnrz32. the esta gene consisted of a 558 bp open reading frame encoding a putative peptide of 21.3 kda. protein sequence homology searches using blast revealed that esta had low amino acid sequence identity with the serine-dependent arylesterases tesi (24%) and etpa (26%) from escherichia coli and vibrio mimicus, respectively. a recombinant esta fusion protein containing a c-terminal si ... | 2000 | 10792515 |
| analysis of vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction. | a total of 51 vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (ap-pcr). the primer vmh-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. all groups had a common 1.0-kb amplification fragment. most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. ap-pcr groups we ... | 2000 | 10803502 |
| the vibrio cholerae o1 chromosomal integron. | until the discovery of the vibrio cholerae repeat (vcr), the gene capture and expression systems termed integrons had been typically associated with antibiotic-resistance gene cassettes with usually less than five genes in an array. a method is described for the cloning of the ends of large cassette arrays. conserved restriction sites within vcrs facilitated the mapping by southern hybridization and cloning of the 5' end of the vcr array, and using appropriate fragments it was possible to develo ... | 2000 | 11021935 |
| identification and characterization of the soda genes encoding manganese superoxide dismutases in vibrio parahaemolyticus, vibrio mimicus, and vibrio vulnificus. | sequencing of fur titration assay-positive clones obtained from genomic dna libraries of vibrio parahaemolyticus, v. mimicus and v. vulnificus revealed open reading frames encoding proteins of 202, 205 and 202 amino acid residues, respectively. each open reading frame was preceded by a predicted fur box which overlaps a likely promoter with similarity to the -10 and -35 consensus sequence of escherichia coli. the deduced amino acid sequences shared considerable homology with bacterial mn-contain ... | 2001 | 11293479 |