| characterization of biochemically atypical vibrio cholerae strains and designation of a new pathogenic species, vibrio mimicus. | biochemically atypical strains classified as vibrio cholerae were characterized by biochemical reactions, serology, antibiotic susceptibility testing, and deoxyribonucleic acid relatedness. strains with the following atypical reactions were shown to be v. cholerae: mannose negative, mannitol negative, lysine decarboxylase negative, no growth in the presence of 5% nacl, salicin and cellobiose positive. sucrose-negative strains were shown to constitute a new species, vibrio mimicus, whose type str ... | 1981 | 7037833 |
| gene expression and genetic engineering in the lens. friedenwald lecture. | | 1987 | 3026987 |
| [in vitro antibacterial activity of balofloxacin (blfx) against isolates from patients with bacterial enteritis]. | in vitro antibacterial activity of balofloxacin (blfx), a newly developed fluoroquinoline, was compared with that of norfloxacin (nflx), ofloxacin (oflx) and ciplofloxacin (cpfx). bacterial strains used in this experiment were freshly isolated from patients with infectious enteritis just before blfx therapy. the isolates were 43 strains of vibrio cholerae o1, 1 strain of campylobacter sp., 4 strains of aeromonas spp., 3 strains of plesiomonas shigelloides, 1 strain of vibrio mimicus and 1 strain ... | 1995 | 7594800 |
| role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats. | permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ... | 1987 | 3032567 |
| seasonal distribution of facultatively enteropathogenic vibrios (vibrio cholerae, vibrio mimicus, vibrio parahaemolyticus) in the freshwater of the elbe river at hamburg. | between june 1981 and december 1982 the incidence of vibrio cholerae, v. mimicus and v. parahaemolyticus was determined at two sampling sites on the elbe river at hamburg. a total of 183 strains was isolated from 147 water samples. of these, 107 belonged to non-01 v. cholerae (ten strains producing a cholera-like enterotoxin); 33 were identified as v. mimicus, including two enterotoxin producers; 42 strains were kanagawa-negative cultures of v. parahaemolyticus; and one was v. fluvialis. the hig ... | 1986 | 3722029 |
| fatal gastroenteritis due to vibrio fluvialis and nonfatal bacteremia due to vibrio mimicus: unusual vibrio infections in two patients. | | 1994 | 7811875 |
| existence of a novel hemagglutinin having no protease activity in vibrio mimicus. | the protease elaborated by vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known ha/protease. a non-protease hemagglutinin (ha) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of v. mimicus. this ha (v. mimicus ha: vmha) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocy ... | 1994 | 7968677 |
| anticandidal activities of terconazole, a broad-spectrum antimycotic. | terconazole is a new triazole ketal derivative with broad-spectrum in vitro and in vivo antifungal activities. this study further characterizes the effects of terconazole in vitro on yeast cell growth, viability, and morphology. terconazole inhibited the growth of candida albicans atcc 44859 in a concentration-related manner, but with modest effects noted at levels from 10(-8) to 10(-5) m when the yeast was grown on media favoring the cell form. the inhibitory potency of terconazole on yeast cel ... | 1986 | 3729366 |
| a gene for the enterotoxin zonula occludens toxin is present in vibrio mimicus and vibrio cholerae o139. | the presence of the zonula occludens toxin (zot) gene, which encodes an enterotoxin produced by serotype o1 strains of the pathogenic bacterium, vibrio cholerae, in addition to cholera toxin, was investigated in selected strains of v. mimicus and the new pandemic v. cholerae non-o1 serotype o139. the zot gene was detected by polymerase chain reaction (pcr) amplification, using sets of primers based on the sequence of the v. cholerae o1 zot sequence. pcr amplification of genomic dnas of both chol ... | 1994 | 8050719 |
| the binding of pirenzepine to digitonin-solubilized muscarinic acetylcholine receptors from the rat myocardium. | the binding of pirenzepine to digitonin-solubilized rat myocardial muscarinic acetylcholine receptors has been examined at 4 degrees c. solubilization produced only small changes in the binding of n-methylscopolamine and atropine. in contrast to the low affinity binding of pirenzepine found to be present in in the membranes, high affinity binding was detected in the soluble preparation. in both preparations, pirenzepine binding was complex. high affinity pirenzepine binding (kd approximately 3 x ... | 1986 | 3754173 |
| regional myocardial protection by retrograde coronary sinus infusion of cardioplegic solution. | antegrade administration of a cardioplegic solution in the presence of a coronary artery stenosis may lead to the heterogeneous distribution of the agent and poor myocardial cooling distal to a vessel stenosis. to determine the effects of retrograde coronary sinus infusion of cardioplegic solution, coronary stenosis was created in the canine preparation by occluding the left circumflex artery (lcx) during cardioplegic arrest. left ventricular global and regional function (assessed by sonomicrome ... | 1986 | 3769184 |
| 1h-15n heteronuclear nmr studies of escherichia coli thioredoxin in samples isotopically labeled by residue type. | ten samples of escherichia coli thioredoxin were individually isotopically enriched by residue type via growth of an appropriate auxotrophic strain on media supplemented with one 2h, 15n-enriched amino acid. 1h observe-heteronuclear decoupling experiments were conducted on these samples making use of the 95-hz 1h-15n amide j1 coupling. subtraction of near-resonance from off-resonance 15n decoupled spectra generated difference patterns corresponding only to protons directly bonded to 15n nuclei. ... | 1985 | 3910099 |
| coagglutination of vibrio cholerae, vibrio mimicus, and vibrio vulnificus with anti-flagellar monoclonal antibody. | monoclonal antibodies (mabs) with serological activity for purified flagellar (h) core protein prepared from vibrio cholerae were identified by enzyme-linked immunosorbent assay. four of these mabs reacted with the flagella of v. cholerae and v. mimicus exclusively, while eight mabs reacted with at least 1 of 30 heterologous vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. it appears that v. cholerae and v. mimicus express similar, if not identical, h determinants u ... | 1988 | 3182988 |
| vibrio mimicus. | | 1993 | 8294347 |
| acute diarrhoea caused by vibrio mimicus in calcutta. | out of 539 acute diarrhoea cases studied, vibrio mimicus was isolated as a sole pathogen in the faeces of 7 (1.3%) cases. the chief clinical presentations of the seven cases were watery diarrhoea and vomiting. bloody diarrhoea was observed in 2 (28.5%), abdominal pain in 2 (28.57%) and fever in one (14.29) cases. all cases could be effectively treated with ors except 3 (42.85%) cases who required iv ringer's lactate. all v. mimicus strains isolated in the study were uniformly susceptible to tetr ... | 1993 | 8294349 |
| structural studies of the vibrio mimicus w-26768 o-antigen polysaccharide. | the structure of the vibrio mimicus w-26768 o-antigen polysaccharide has been investigated by sugar and methylation analyses, smith degradation, and nmr spectroscopy. it is proposed that it is composed of chains of beta-(1-->4)-linked 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucopyranosyl residues (d-qui(p)3nr), approximately 60% of which are substituted in the 2-position with 2-acetamido-2-deoxy-alpha-d-galactopyranosyl groups. the polysaccharide does not seem to be composed of oligosaccharid ... | 1993 | 8324759 |
| hypergammaglobulinemia associated with human immunodeficiency virus infection. | the serum concentrations of 11 ig isotypes (igg, igg1, igg2, igg3, igg4, iga, iga1, iga2, igm, igd, and ige) were measured in four relatively small groups of homosexual (or bisexual) males. all these patients were seropositive for hiv. two of the groups (nonprogressors) were clinically stable for approximately 2 years and were characterized either as asymptomatic or with pgl. the third group (progressors) developed aids 2-38 months after blood specimens were taken. the fourth group had aids. a f ... | 1988 | 3260340 |
| studies on hemolytic action of a hemolysin produced by vibrio mimicus. | some properties and mechanism of action of a hemolysin (vmh) produced by an enteropathogenic vibrio mimicus strain was examined. vmh was heat-labile and inhibited by addition of divalent cations, including ca2+, mg2+ and mn2+. the hemolysis by vmh was inhibited by incubating with gangliosides, suggesting that the ganglioside was the binding site on the erythrocyte membrane for vmh. existence of a galactose moiety on reducing end of the ganglioside molecule and a sialic acid on the galactose moie ... | 1993 | 8355623 |
| phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences. | the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ... | 1993 | 8427811 |
| structural and functional studies of the amino terminus of yeast metallothionein. | purified yeast copper-metallothionein lacks 8 amino-terminal residues that are predicted from the dna sequence of its gene. the removed sequence is unusual for metallothionein in its high content of hydrophobic and aromatic residues and its similarity to mitochondrial leader sequences. to study the significance of this amino-terminal cleavage, several mutations were introduced into the metallothionein coding gene, cup1. one mutant, which deletes amino acid residues 2-8, had a minor effect on the ... | 1987 | 3308865 |
| simultaneous measurement of preferred ambient temperature and metabolism in rats. | a recent study from this laboratory found that rats placed in a temperature gradient preferred ambient temperatures (ta) that were significantly below the lower critical ta of the thermoneutral zone for elevating metabolic rate (mr). to further evaluate the interaction between preferred ta and mr in the rat, a system was developed where preferred ta, mr (i.e., o2 consumption), and activity could be automatically monitored in the unrestrained rat. two groups of male sprague-dawley rats, a light-w ... | 1988 | 3344833 |
| site-directed mutagenesis of a novel serine arylesterase from vibrio mimicus identifies residues essential for catalysis. | site-directed mutagenesis (sdm) of an arylesterase (the arylesterase) from vibrio mimicus revealed that residues s29, h153, and d96 constituted a catalytic triad. the use of a serine residue for ester hydrolysis by the arylesterase proves that the enzyme is a novel serine arylesterase. sdm also showed that d28 was necessary for the esterase activity; to our knowledge it is the first time that a residue immediately preceding the active-site serine in esterases was shown biochemically to possess s ... | 1996 | 8619880 |
| expression of virulence-related properties by, and intestinal adhesiveness of, vibrio mimicus strains isolated from aquatic environments. | a study of the major pathogenic characteristics of vibrio mimicus was carried out with 77 strains isolated from aquatic environments in okayama, japan. of the strains tested, 96% demonstrated in vitro adherence to the rabbit intestinal mucosa, of which 36, 20, and 43% belonged to the strongly, moderately, and weakly adhesive groups, respectively. of the 27 strains which appeared to be enterotoxigenic in the experiments using rabbit ileal loops, 74% belonged to the strongly adhesive group. all st ... | 1996 | 8837444 |
| trimethoprim used for selective decontamination of the digestive tract in rats: possible route of excretion. | selective elimination of enterobacteriaceae species from the digestive tract of rats has been accomplished by oral treatment with trimethoprim (235 mg/kg body weight/day) within 6 days. in the present study it was investigated whether this elimination was mainly due to antimicrobial activity of trimethoprim excreted with the gastrointestinal mucus or mainly by non-absorbed trimethoprim in the lumen contents. by means of whole gut irrigation (wgi) the lumen contents were washed out, followed by m ... | 1987 | 3497436 |
| effect of motion on the sonographic and magnetic resonance patterns of ageing blood. | the sonographic appearance of a hematoma may be affected by various factors, including the age of the hematoma. the effect of mechanical motion on the echogenicity and histologic appearance, and on the proton relaxation times t1 and t2 of blood clots, was studied in vitro for up to 21 days. all clots were of similar echogenicity and microscopic appearance during the first 2 days. the minimally disturbed clots were sonolucent from day 4 onwards, whereas moderate mechanical disturbance changed the ... | 1986 | 3535388 |
| nocardia asteroides as a cause of corneal ulcer. case report. | | 1987 | 3551895 |
| the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei. | fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ... | 1987 | 3554006 |
| pentapeptide nuclear localization signal in adenovirus e1a. | the adenovirus e1a gene products are nuclear proteins important in transcriptional control of viral functions during infection. by producing normal e1a proteins and derivatives of e1a in bacteria and microinjecting these proteins into cultured cells, we were able to examine their ability to localize to the nucleus. we showed that a short peptide sequence at the carboxyl terminus of e1a is necessary for the rapid (30-min) nuclear localization of that protein. additionally, we showed that just the ... | 1987 | 3614197 |
| toxin production by vibrio mimicus strains isolated from human and environmental sources in bangladesh. | vibrio mimicus has recently been isolated from aquatic environments of bangladesh. a total of 125 of 300 environmental isolates, representing various biotypes, and 19 human isolates were tested for enteropathogenicity by using several models. less than 1% of the environmental isolates and slightly more than 10% of the clinical isolates produced cholera toxin-like toxin. a significant percentage of the environmental isolates (25%) and of the human isolates (74%) induced fluid accumulation in liga ... | 1987 | 3693548 |
| growth hormone modulates serum cholinesterase. | adult male rats have lower serum cholinesterase activity levels than adult female rats and hypophysectomized male rats have higher activity levels than sham-operated males (similar to control females). gh administered to hypophysectomized male rats abolishes the effect of hypophysectomy on serum cholinesterase. adult male rats treated neonatally with monosodium l-glutamate to induce arcuate nucleus lesions of the hypothalamus have higher serum cholinesterase activities and decreased serum gh con ... | 1986 | 3948777 |
| the distribution of lactate dehydrogenase isoenzymes in adult human achilles tendons. | the authors have studied the ldh isoenzymes distribution in the human achilles tendons. connection between the age, sex, and enzyme activity were not found. in the achilles tendon were found strong activity of isoenzymes iv and v (m-type), and controversy them moderate activity of isoenzymes i and ii (h-type). | 1985 | 4065385 |
| structural analysis of the o-antigenic polysaccharide from vibrio mimicus n-1990. | the o-antigenic polysaccharide part of the lipopolysaccharide from vibrio mimicus n-1990 has been investigated. sugar and methylation analysis of native and dephosphorylated polysaccharide together with nmr spectroscopy show that the polysaccharide is composed of tetrasaccharide repeating units. the structure of the repeating unit of the polysaccharide from v. mimicus n-1990 could be determined as: -->4)-alpha-d-galpnac-(1-->3)-beta-d-galpnac-(1-->2)-4,6-p-beta-d-galp-( 1-->3)-alpha-d-galpnac-(1 ... | 1998 | 9490076 |
| experimental studies on the pathogenicity of vibrio mimicus strains isolated in bangladesh. | vibrio mimicus, a newly described species of the genus vibrio has been isolated from stools of 14 patients with diarrhoea. live cells of all the 14 strains tested caused accumulation of fluid in rabbit gut loops and diarrhoea in infant rabbits. culture filtrates of all the strains caused increased capillary permeability in rabbit skin; however, five of the filtrates resembled cholera toxin in that they gave positive reactions in rabbit loops, chinese hamster ovarian and mouse adrenal cell monola ... | 1984 | 6517767 |
| production of cholera toxin-like toxin by vibrio mimicus and non-o1 vibrio cholerae: batch culture conditions for optimum yields and isolation of hypertoxigenic lincomycin-resistant mutants. | vibrio mimicus 61892, isolated in 1977 from a case of watery diarrhea in bangladesh, produces an enterotoxin which possesses activity in y-1 mouse adrenal cells and in rabbit ileal loops which is identical to the prototype cholera toxin (ct) produced by vibrio cholerae 569b. the neutralization of the adrenal cell activity of 61892 toxin and 569b ct by homologous and heterologous antisera generates parallel titration curves which show complete neutralization in all cases. paired titrations in the ... | 1983 | 6642640 |
| ecology of non-o 1 vibrio cholerae in toyama prefecture. | the ecology of non-o 1 vibrio cholerae and vibrio mimicus as causes of cholera-like diarrhea or seafood-associated gastroenteritis has been investigated in toyama prefecture since 1980. the relationship between biological or serological characteristics of the isolates and their enteropathogenicity is discussed. overall isolation rates from river water, sea water, and fish were 24.0, 59.5, and 33.7%, respectively, the isolation frequency being, in general, extremely high in the summer season, alt ... | 1984 | 6738382 |
| sources of vibrio mimicus contamination of turtle eggs. | vibrio mimicus contamination of sand increased significantly during the arrival of the olive ridley sea turtles (lepidochelys olivacea) at ostional anidation beach, costa rica. statistical analysis supports that eggs are contaminated with v. mimicus by contact with the sand nest. v. mimicus was isolated from eggs of all nests tested, and ctxa+ strains were found in 31% of the nests, all of which were near the estuary. | 1999 | 9872804 |
| disease from infection with vibrio mimicus, a newly recognized vibrio species. | clinical and epidemiologic characteristics of disease associated with a newly described nonhalophilic vibrio species, vibrio mimicus, were identified by studying isolates from 21 patients referred to the centers for disease control between 1977 and 1981. two isolates were from the ears of patients with otitis who had recently been exposed to seawater. nineteen isolates were from stool samples; these patients generally had diarrhea, nausea, vomiting, and abdominal cramps, with fever, headache, an ... | 1983 | 6881769 |
| disease from infection with vibrio mimicus, a newly recognized vibrio species. clinical characteristics and edipemiology. | clinical and epidemiologic characteristics of disease associated with a newly described nonhalophilic vibrio species, vibrio mimicus, were identified by studying isolates from 21 patients referred to the centers for disease control between 1977 and 1981. two isolates were from the ears of patients with otitis who had recently been exposed to seawater. nineteen isolates were from stool samples; these patients generally had diarrhea, nausea, vomiting, and abdominal cramps, with fever, headache, an ... | 1983 | 6881770 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| iron transport genes of the pjm1-mediated iron uptake system of vibrio anguillarum are included in a transposonlike structure. | the pjm1 genes encoding the proteins involved in iron transport in the anguibactin iron uptake system were found to be flanked by insertion sequences in a composite transposonlike structure. these vibrio anguillarum insertion sequences, isv-a1 and isv-a2, are related to is903, is102, and the isvs found in vibrio parahaemoliticus, vibrio mimicus, and non-o1 vibrio cholerae flanking various tdh (thermostable direct hemolysin) genes. the inverted repeats at the ends of isv-a1 and isv-a2 have no mor ... | 1995 | 7568465 |
| identification of the siderophores from vibrio hollisae and vibrio mimicus as aerobactin. | hydroxamate siderophores were purified from low-iron cultures of vibrio hollisae atcc 33564 and vibrio mimicus atcc 33653. by a combination of 1h and 13c nmr spectroscopy, fast atom bombardment mass spectrometry, and compositional analysis, both of the siderophores were identified as aerobactin, a citrate-based dihydroxamate siderophore, which is highly prevalent in species of the family enterobacteriaceae. four other clinical strains belonging to these species also produced aerobactin. in respo ... | 1994 | 8013878 |
| an analysis of the v1 and v2 regions of vibrio cholerae and vibrio mimicus 16s rrna. | the v1 and v2 variable regions of the 16s rrna gene of three strains of v. cholerae and one strain of v. mimicus were amplified by pcr. fragments containing both regions were cloned into m13mp18 using smal and sequenced by the dideoxy method. the 263-bp sequence from a strain isolated during the 1991 cholera outbreak in brazil was deposited in genbank under the accession number l05178. except for an extra g in one of the strains, the three v. cholerae sequences were identical. the v. mimicus seq ... | 1994 | 8090995 |
| vibrio mimicus with multiple toxin types isolated from human and environmental sources. | a collection of 13 strains of vibrio mimicus, including both clinical and environmental isolates from different geographic regions, was examined for various toxins. one strain of environmental origin produced cholera-like toxin (ct) which was completely absorbed with anti-ct immunoglobulin g, five strains produced a haemolysin that cross-reacted with the thermostable direct haemolysin of v. parahaemolyticus and dna from two strains hybridised with a dna probe specific for the heat-stable enterot ... | 1994 | 8114069 |
| evaluation of virulence factors in environmental isolates of vibrio species. | strains of vibrio parahaemolyticus, vibrio fluvialis and vibrio mimicus isolated from seafood and seawater were examined for characteristics related to infectivity, such as enzymatic activity and animal assays. all strains hydrolysed dna, starch, gelatin and chitin. variable results were obtained with the haemolysin, chondroitin, collagen, elastin and lecithin tests. production of thermostable direct haemolysin by v. parahaemolyticus was detected in 7.1% strains derived from seafood and 2% from ... | 1993 | 8139466 |
| nucleotide sequence of a novel arylesterase gene from vibro mimicus and characterization of the enzyme expressed in escherichia coli. | a gene coding for an arylesterase of vibrio mimicus was cloned. sequence determination reveals that the esterase gene has an open reading frame of 600 nucleotides which encodes a protein of m(r) 22,300. the deduced amino acid sequence contain a pentapeptide gdsls (residues 27-31), which was also found in the phospholipid-cholesterol acyltransferase from aeromonas hydrophila. substitution of ser-29 by alanine or cysteine in the cloned gene abolished the esterase activity in the tributyrin plate a ... | 1994 | 8141782 |
| high prevalence of thermostable direct hemolysin (tdh)-like toxin in vibrio mimicus strains isolated from diarrhoeal patients. | a total of 17 isolates of vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. sixteen (94%) clinical isolates and one (50%) from food produced tdh-like toxin, whereas none of the environmental isolates did so. the food from which v. mimicus with tdh-like toxin production was isolated, was one which had caused food poisoning. only one environmental strain produced ct-like toxin, whilst st-like toxin was not detected from any strains tested. | 1993 | 8348932 |
| differentiation of environmental and clinical isolates of vibrio mimicus from vibrio cholerae by multilocus enzyme electrophoresis. | in this study, we demonstrated that analyzed strains of vibrio mimicus and vibrio cholerae could be separated in two groups by using multilocus enzyme electrophoresis (mee) data from 14 loci. we also showed that the combination of four enzymatic loci enables us to differentiate these two species. our results showed that the ribosomal intergenic spacer regions pcr-mediated identification system failed, in some cases, to differentiate between v. mimicus and v. cholerae. on the other hand, mee prov ... | 2001 | 11319123 |
| development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene. | an alkaline phosphatase-conjugated 30-mer oligonucleotide probe was developed to detect the cholera toxin gene (ctx) in vibrio cholerae o1. for rapid identification, v. cholerae o1 was grown on selective agar (thiosulfate-citrate-bile salts agar) or in alkaline peptone water and organisms were transferred directly to nylon membranes. lysis of cells, denaturation of dna, neutralization, and hybridization were carried out on the membrane. these procedures required only 3 h for completion. the resu ... | 1993 | 8501233 |
| characterization of aeromonas and vibrio species isolated from a drinking water reservoir. | to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species. | 2001 | 11412322 |
| crystallization and preliminary x-ray crystallographic analysis of arylesterase from vibrio mimicus. | single crystals of arylesterase (ec 3.1.1.2) from vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. the present crystals diffract up to 2.2 a resolution and belong to monoclinic space group p2(1). the cell dimensions are a = 55.65(1) a, b = 53.46(1) a, c = 65.79(1) a, and beta = 106.54(1) degrees. there are two molecules of molecular weight 22 kda in an asymmetric unit with a solvent content of 43%. | 1995 | 8528091 |
| vibrio mimicus diarrhea following ingestion of raw turtle eggs. | clinical and epidemiological characteristics of diarrhea associated with vibrio mimicus were identified in 33 hospitalized patients referred to the costa rican national diagnostic laboratory network between 1991 and 1994. the relevant symptoms presented by patients included abundant watery diarrhea, vomiting, and severe dehydration that required intravenous dhaka solution in 83% of patients but not fever. seroconversion against v. mimicus was demonstrated in four patients, from whom acute- and c ... | 1996 | 8919774 |
| analysis of 16s-23s rrna intergenic spacer of vibrio cholerae and vibrio mimicus for detection of these species. | | 2002 | 11692861 |
| purification and characterization of novel hemagglutinins from vibrio mimicus: a 39-kilodalton major outer membrane protein and lipopolysaccharide. | two hemagglutinins (has) mediating the agglutinability to rabbit erythrocytes were isolated from 32-h culture supernatant of enterotoxigenic strain e-33 of vibrio mimicus by ultrafiltration followed by gel filtration and anion-exchange column chromatography. the has were designated r-ha and c-ha on the basis of specific hemagglutinating activity towards rabbit erythrocytes only (r-ha) and towards chicken and rabbit erythrocytes (c-ha). sodium dodecyl sulfate-polyacrylamide gel electrophoresis an ... | 1996 | 8926065 |
| toxigenicity & drug sensitivity of vibrio mimicus isolated from patients with diarrhoea. | in a total of 720 faecal specimens from patients with secretory diarrhoea, vomiting, dehydration, gastroenteritis, cholera and cholera like illnesses, 18 strains of v. mimicus were isolated as pure culture. these were characterized for various toxin types and virulence factors using conventional in vitro and in vivo assays. labile and stable toxins were elaborated by 15 and 2 strains respectively by ligated rabbit ileal loop (ril) and suckling mouse assays. while 15 of the whole cell culture ela ... | 1996 | 8996933 |
| analysis of the structural gene encoding a hemolysin in vibrio mimicus. | an environmental isolate of v. mimicus, strain e-33, has been reported to produce and secrete a hemolysin of 63 kda. the hemolysin is enterotoxic in test animals. the nucleotide sequence of the structural gene of the hemolysin was determined. we found a 2,232 bp open reading frame, which codes a peptide of 744 amino acids, with a calculated molecular weight of 83,903 da. the sequence for the structural gene was closely related to the v. cholerae el tor hlya gene, coding an exocellular hemolysin. ... | 1997 | 9087960 |
| development of a hexaplex pcr assay for rapid detection of virulence and regulatory genes in vibrio cholerae and vibrio mimicus. | we have developed a hexaplex pcr assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit a (ctxa), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpa), outer membrane protein (ompu), and central regulatory protein toxr (toxr) in vibrio cholerae and vibrio mimicus. this hexaplex pcr proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic v. cholerae and v. mimicus strains from both ... | 2002 | 12409420 |
| purification and characterization of a hemolysin produced by vibrio mimicus. | vibrio mimicus is a causative agent of human gastroenteritis. this pathogen secretes a pore-forming toxin, v. mimicus hemolysin (vmh), which causes hemolysis by three sequential steps: binding to an erythrocyte membrane, formation of a transmembrane pore, and disruption of the cell membrane. vmh with a molecular mass of 63 kda was purified by ammonium sulfate precipitation and column chromatography with phenyl sepharose hp and superose 6 hr. the hemolytic reaction induced by vmh continued up to ... | 1997 | 9125568 |
| nucleotide sequence of the vmha gene encoding hemolysin from vibrio mimicus. | the structural gene (vmha) of hemolysin from vibrio mimicus (atcc33653) was cloned and sequenced. the vmha gene contains an open reading frame consisting of 2232 nucleotides which can code for a protein of 744 amino acids with a predicted molecular mass of 83,059. the similarity of amino acid sequence shows 81.6% identity with vibrio cholerae el tor hemolysin. | 1997 | 9128176 |
| vibrio mimicus attaches to the intestinal mucosa by outer membrane hemagglutinins specific to polypeptide moieties of glycoproteins. | vibrio mimicus is the closest organism to vibrio cholerae. v. mimicus e-33, which is a highly adhesive and enteropathogenic strain, is known to produce three types of hemagglutinins (has), i.e., a 31-kda exocellular metalloprotease (vm-ha/protease), lipopolysaccharide (vm-lpsha), and a 39-kda major outer membrane protein (vm-ompha). hemagglutination induced by vm-lpsha and vm-ompha was inhibited by glycoproteins, including mucin, fetuin, and asialofetuin, but not by monosaccharides, disaccharide ... | 1997 | 9284134 |
| use of reca as an alternative phylogenetic marker in the family vibrionaceae. | this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ... | 2004 | 15143042 |
| [distribution of serogroups of vibrio cholerae non-o1 non-o139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups]. | a total of 1898 strains of vibrio cholerae non-o1 non-o139, which had been collected worldwide for the past 3 year period of 1994-1996, were serogrouped. the strains were also examined for presence of cholera toxin (ct) gene (ctx) and nag-st gene, and strains which carried to ctx were further analyzed for their ability to produce ct. in addition, attempts were made to establish novel serogroups for those serologically untypable strains. of those examined, 1,774 strains of v. cholerae non-o1 non- ... | 1997 | 9394556 |
| distribution of virulence-associated genes in vibrio mimicus isolates from clinical and environmental origins. | distribution of virulence-associated genes in vibrio mimicus was studied including the toxin genes ctxa, tdh, st and vmh and the genes necessary for regulation of toxin production, toxr, toxs, toxt, tcpa and tcpp. approximately half of clinical v. mimicus isolates possessed one or more genes encoding v. cholerae enterotoxic factors such as ctxa, tdh and st. all of the clinical and environmental isolates possessed vmh encoding v. mimicus hemolysin (vmh). the ctxa encoding cholera toxin was detect ... | 2004 | 15272201 |
| isolation and sequence analysis of metalloprotease gene from vibrio mimicus. | the vmc gene encoding a metalloprotease of vibrio mimicus (atcc 33653) was cloned in escherichia coli and sequenced. the vmc gene contained 1884 nt sequence which codes a polypeptide of 628 amino acids with a predicted molecular mass of 71,275 da. the deduced amino acid sequence had the similarity of 68.5% with v. parahaemolyticus metalloprotease. the consensus sequence of a zinc binding motif (hexxh) was identified to be heyth. the zymography analysis showed a gelatinolytic protein band around ... | 1998 | 9602021 |
| cloning and identification of a phospholipase gene from vibrio mimicus. | the phospholipase gene phl was identified from vibrio mimicus (atcc33653) and sequenced. the entire open reading frame (orf) was composed of 1410 nucleotides and encoding 470 amino acids. the phl was placed upstream of hemolysin gene (vmha) with opposite direction of transcription. from the blast search program, the deduced amino acids sequence showed 74.4% identity with phospholipase gene (lec) from v. cholerae el tor. the entire orf of phospholipase gene was amplified by pcr and inserted into ... | 1998 | 9767128 |
| optimization of excimer-forming two-probe nucleic acid hybridization method with pyrene as a fluorophore. | a previously presented homogeneous assay method, named the excimer-forming two-probe nucleic acid hybridization (etph) method, is based on specific excimer formation between two pyrenes attached at the neighboring terminals of two sequential probe oligonucleotides complementary to a single target. in this study, we investigated assay conditions and optimal molecular design of probes for intense excimer emission using a pyrenemethyliodoacetamide-introduced 16mer probe, a pyrene butanoic acid-intr ... | 1998 | 9826766 |
| detection of genes encoding cholera toxin (ct), zonula occludens toxin (zot), accessory cholera enterotoxin (ace) and heat-stable enterotoxin (st) in vibrio mimicus clinical strains. | a total of 51 clinical strains of vibrio mimicus were searched for the presence of virulence-associated genes, like ctx, zot or ace genes which locate in "cholera virulence cassette," and the st gene by polymerase chain reaction. moreover, the pathological potential of each clinical strain was also examined by rabbit ileal loop (ril). three strains showed to have the ctx gene, of which only one strain was zot gene-positive. meanwhile, one other strain was zot+ but ctx-. all of these four strains ... | 1998 | 10037216 |
| molecular typing of epidemic and nonepidemic vibrio cholerae isolates and differentiation of v. cholerae and v. mimicus isolates by pcr-single-strand conformation polymorphism analysis. | to examine the utility of polymerase chain reaction (pcr)-single-strand conformation polymorphism (sscp) analysis to differentiate epidemic and nonepidemic vibrio cholerae isolates as well as to differentiate v. cholerae and vibrio mimicus isolates. | 2005 | 15715856 |
| vibrio mimicus arylesterase has thioesterase and chymotrypsin-like activity. | a vibrio mimicus serine arylesterase and an escherichia coli thioesterase/serine protease share 49.4% amino acid identity. the arylesterase has thioesterase activity for benzoyl-coa and chymotrypsin-like activity for n-carbobenzoxy-l-phenylalanine p-nitrophenyl ester (nbpnpe). the gene encoding the v. mimicus enzyme is designated etpa. substituting ser31 of the v. mimicus enzyme with a glycine or an alanine altered its activity. in comparison with wild type enzyme, the s31a enzyme showed a 5-fol ... | 1995 | 7646502 |
| the sixth and seventh cholera pandemics are due to independent clones separately derived from environmental, nontoxigenic, non-o1 vibrio cholerae. | the dna sequences of the asd genes from 45 isolates of vibrio cholerae (19 clinical o1 isolates, 2 environmental nontoxigenic o1 isolates, and 24 isolates with different non-o1 antigens) were determined. no differences were found within either sixth- or seventh-pandemic isolates; however, variation was found between the two forms and among the non-o1 isolates. o139 isolates had sequences identical to those of seventh-pandemic isolates. phylogenetic trees with vibrio mimicus as the outgroup sugge ... | 1995 | 7768818 |
| infectious ctxphi and the vibrio pathogenicity island prophage in vibrio mimicus: evidence for recent horizontal transfer between v. mimicus and v. cholerae. | vibrio mimicus differs from vibrio cholerae in a number of genotypic and phenotypic traits but like v. cholerae can give rise to diarrheal disease. we examined clinical isolates of v. mimicus for the presence of ctxphi, the lysogenic filamentous bacteriophage that carries the cholera toxin genes in epidemic v. cholerae strains. four v. mimicus isolates were found to contain complete copies of ctxphi. southern blot analyses revealed that v. mimicus strain pt5 contains two ctx prophages integrated ... | 2000 | 10678967 |
| selectivity and specificity of a chromogenic medium for detecting vibrio parahaemolyticust. | the thiosulfate-citrate-bile salts-sucrose agar (tcbs) used in the most-probable-number method for detecting vibrio parahaemolyticus cannot differentiate growth of v. parahaemolyticus from vibrio vulnificus or vibrio mimicus. this study examined the selectivity and specificity of bio-chrome vibrio medium (bcvm), a chromogenic medium that detects v. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. a panel consisting of 221 strains of bacteria, including 17 ... | 2005 | 16013386 |
| characterization of an arylesterase from lactobacillus helveticus cnrz32. | an esterase gene (esta) was isolated from a previously constructed genomic library of lactobacillus helveticus cnrz32. the esta gene consisted of a 558 bp open reading frame encoding a putative peptide of 21.3 kda. protein sequence homology searches using blast revealed that esta had low amino acid sequence identity with the serine-dependent arylesterases tesi (24%) and etpa (26%) from escherichia coli and vibrio mimicus, respectively. a recombinant esta fusion protein containing a c-terminal si ... | 2000 | 10792515 |
| analysis of vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction. | a total of 51 vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (ap-pcr). the primer vmh-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. all groups had a common 1.0-kb amplification fragment. most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. ap-pcr groups we ... | 2000 | 10803502 |
| use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media. | identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ... | 2005 | 16300063 |
| the vibrio cholerae o1 chromosomal integron. | until the discovery of the vibrio cholerae repeat (vcr), the gene capture and expression systems termed integrons had been typically associated with antibiotic-resistance gene cassettes with usually less than five genes in an array. a method is described for the cloning of the ends of large cassette arrays. conserved restriction sites within vcrs facilitated the mapping by southern hybridization and cloning of the 5' end of the vcr array, and using appropriate fragments it was possible to develo ... | 2000 | 11021935 |
| high prevalence of vibrio cholerae non-o1 carrying heat-stable-enterotoxin-encoding genes among vibrio isolates from a temperate-climate river basin of central italy. | vibrio spp. of clinical interest from the arno river basin (tuscany, italy) were investigated in this study. vibrios were isolated from 70% of water samples. vibrio cholerae non-o1 was the most prevalent species (82% of isolates), followed by vibrio mimicus (10%) and vibrio metschnikovii (8%). recovery of vibrios was correlated with temperature, ph, and various indicators of municipal pollution. none of the 150 vibrio isolates carried ctx-related genomic sequences, whereas 18 (14.6%) of the 123 ... | 1997 | 16535661 |
| identification and characterization of the soda genes encoding manganese superoxide dismutases in vibrio parahaemolyticus, vibrio mimicus, and vibrio vulnificus. | sequencing of fur titration assay-positive clones obtained from genomic dna libraries of vibrio parahaemolyticus, v. mimicus and v. vulnificus revealed open reading frames encoding proteins of 202, 205 and 202 amino acid residues, respectively. each open reading frame was preceded by a predicted fur box which overlaps a likely promoter with similarity to the -10 and -35 consensus sequence of escherichia coli. the deduced amino acid sequences shared considerable homology with bacterial mn-contain ... | 2001 | 11293479 |
| presence of luxs/ai-2 based quorum-sensing system in vibrio mimicus : luxo controls protease activity. | presence of the quorum-sensing regulation system in vibrio mimicus was investigated. the culture supernatants of v. mimicus strains were found to possess ai-2 autoinducer like activity, and the strains were found to harbor the genes which are homologous to luxs, luxo, and luxr of v. harveyi. these genes of v. harveyi have been shown to be important components of v. harveyi-like quorum-sensing system. the luxo gene homologue known to encode luxo, the central component of the regulation system, wa ... | 2006 | 16714849 |
| development of monoclonal antibodies for simple identification of vibrio alginolyticus. | the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp. | 2006 | 16965376 |
| synthesis of esculentin-1 antibacterial peptide fragments on 1,4-butanediol dimethacrylate cross-linked polystyrene support. | peptide segments corresponding to antibacterial esculentin-1 (1-15), (3344), (9-27), and their modified forms were synthesized on 1,4-butanediol dimethacrylate cross-linked polystyrene (ps-bdodma) support. hydroxymethyl and aminomethyl 2% ps-bdodma supports were used for the synthesis. the hmpb linker was appended to the aminomethyl resin using hbtu in presence of hobt and the first amino acid was incorporated using msnt. the conventional fmoc synthetic protocol was used for the synthesis of pep ... | 2001 | 11330345 |
| [vibrio mimicus gastroenteritis: report of 2 cases]. | | 2001 | 11488102 |
| microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy. | microwave irradiation (mwi) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (sem). in this paper we propose two simple and quick techniques for processing bacteria (proteus mirabilis and vibrio mimicus) for sem using mwi. in the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conductivity stain. the reagent used for the conductivity stain was the mordant of a light microscopy staining ... | 2004 | 17061527 |
| detection of virulence associated genes in clinical strains of vibrio mimicus. | a total of 42 clinical strains of vibrio mimicus were examined for the presence of virulence associated genes toxr, toxs, toxt, tcpp, ctx and tcpa by pcr assay. almost all strains were shown to have the toxr gene, while the toxs gene was found in 27 strains. on the other hand, five strains possessed both toxt and tcpp genes, but others had neither. only two strains were positive for amplification of the ctx gene, whereas no pcr product with tcpa primers was detected. the results indicate the inc ... | 2001 | 11592635 |
| characterization of vibrio mimicus phospholipase a (phla) and cytotoxicity on fish cell. | vibrio mimicus is a typical strain of vibrio cholerae and produces a phospholipase (phla) which shares a highly conserved amino acid sequence with the lecithinase (lec) of v. cholerae. the recombinant protein (rphla) produced from the phla gene of v. mimicus was expressed in escherichia coli as his-tag fused protein. the rphla was purified by gel filtration and ni-metal affinity chromatographies. when the action mode was investigated by tlc and gc-ms, the purified rphla protein showed a phosphol ... | 2002 | 12387827 |
| catecholamine-induced stimulation of growth in vibrio species. | to evaluate the effect of norepinephrine (ne) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone ne and related compounds on the growth of vibrio parahaemolyticus and other human-pathogenic vibrio species (vibrio cholerae, vibrio vulnificus, and vibrio mimicus). | 2007 | 17576228 |
| rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene. | a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ... | 2007 | 17614235 |
| seasonality of chesapeake bay bacterioplankton species. | bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ... | 2002 | 12406742 |
| improvement of innate immune responses and defense activity in mitten crab (eriocheir sinensis) by oral administration of beta-glucan. | the beta-glucan (bg), extracted from saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (eriocheir sinensis) at 0, 1, 5 and 10 g bg/kg diet for 28 days, followed by a challenge with vibrio mimicus by intramuscular injection. growth, phenoloxidase, superoxide dismutase, acid phosphatase and alkaline phosphatase activity were monitored after 14 and 28 days. the results showed an immunomodulatory effect and protection against v. mimicus by dietary supplementation of bg. ... | 2008 | 18568402 |
| bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay. | the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ... | 2002 | 12406743 |
| purification and characterization of enterotoxigenic el tor-like hemolysin produced by vibrio fluvialis. | the halophilic bacterium vibrio fluvialis is an enteric pathogen that produces an extracellular hemolysin. this hemolysin was purified to homogeneity by using sequential hydrophobic-interaction chromatography with phenyl-sepharose cl-4b and gel filtration with sephacryl s-200. it has a molecular weight of 63,000 and an isoelectric point of 4.6, and its hemolytic activity is sensitive to heat, proteases, and preincubation with zinc ions. the hemolysin lyses erythrocytes of the eight different ani ... | 2003 | 12761101 |
| characterization of the enzyme activity of an extracellular metalloprotease (vmc) from vibrio mimicus and its c-terminal deletions. | to investigate the enzymatic properties of vibrio mimicus metalloprotease, the mature metalloprotease gene (vmc) was overexpressed in escherichia coli and the recombinant protein (rvmc61) was purified by metal affinity chromatography. rvmc61 showed maximum activity at about 37 degrees c, ph 8. the purified rvmc61 was very specific toward collagen substrates, such as gelatin, type i, ii, and iii collagens and synthetic peptides (cbz-gplgp and cbz-gpggpa). but it did not show degrading activity to ... | 2003 | 12829301 |
| accuracy of six commercially available systems for identification of members of the family vibrionaceae. | six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ... | 2003 | 14662957 |
| identification and characterization of two contiguous operons required for aerobactin transport and biosynthesis in vibrio mimicus. | in response to iron deprivation, vibrio mimicus produces aerobactin as a major siderophore. application of the fur titration assay to a v. mimicus genomic dna library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron-regulated operons. one contains three genes encoding homologs of the escherichia coli fhucdb and the other, five genes encoding homologs of the e. coli iucabcd iuta. construction of the v. mimicus polar disruptants in the respecti ... | 2004 | 15215626 |
| the ability of two different vibrio spp. bacteriophages to infect vibrio harveyi, vibrio cholerae and vibrio mimicus. | to determine the host range of the vibrio harveyi myovirus-like bacteriophage (vhml) and the cholera toxin conversion bacteriophage (ctx phi) within a range of vibrio cholerae and v. mimicus and v. harveyi, v. cholerae and v. mimicus isolates respectively. | 2004 | 15357715 |
| evolutionary genetic analysis of the emergence of epidemic vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles. | vibrio cholerae, the causative agent of cholera, is a natural inhabitant of the aquatic ecosystem. we examined a unique collection of v. cholerae clinical and environmental isolates of widespread geographic distribution recovered over a 60-year period to determine their evolutionary genetic relationships based on analysis of two housekeeping genes, malate dehydrogenase (mdh) and a chaperonin (groel). in addition, the phylogenetic distribution of 12 regions associated with virulence was determine ... | 2004 | 15472325 |
| rna colony blot hybridization method for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria. | a species-specific rna colony blot hybridization protocol was developed for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria in environmental water samples. bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobilized on nylon membranes. a fluorescently labeled oligonucleotide probe targeting a phylogenetic signature sequence of 16s rrna of v. cholerae and v. mimicus was hybridized to rrna molecules immobilized ... | 2009 | 19561182 |
| simultaneous differential detection of human pathogenic and nonpathogenic vibrio species using a multiplex pcr based on gyrb and pnta genes. | to develop a multiplex pcr targeting the gyrb and pnta genes for vibrio species differentiation. | 2010 | 19891709 |
| validation of a method for the detection of five species, serogroups, biotypes and virulence factors of vibrio by multiplex pcr in fish and seafood. | in this work a sequential multiplex pcr system was designed and validated for the detection of most frequent foodborne pathogen vibrio species in fish and seafood (vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginoliticus and vibrio mimicus). the method proposed functions in a hierarchical way, being composed of an end-point multiplex pcr to detect the presence of dna belonging to the studied species, followed by multiplex pcr and fragment analysis allowing the viability ... | 2010 | 19913702 |
| [development of taqman real-time pcr in detection of aeromonas hydrophila]. | to develop a taqman real-time pcr for the detection of aeromonas hydrophila. | 2009 | 19954074 |
| molecular evolution of vibrio pathogenicity island-2 (vpi-2): mosaic structure among vibrio cholerae and vibrio mimicus natural isolates. | vibrio cholerae is a gram-negative rod that inhabits the aquatic environment and is the aetiological agent of cholera, a disease that is endemic in much of southern asia. the 57.3 kb vibrio pathogenicity island-2 (vpi-2) is confined predominantly to toxigenic v. cholerae o1 and o139 serogroup isolates and encodes 52 orfs (vc1758 to vc1809), which include homologues of an integrase (vc1758), a restriction modification system, a sialic acid metabolism gene cluster (vc1773-vc1783), a neuraminidase ... | 2005 | 15632448 |