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membrane filter procedure for enumeration of vibrio parahaemolyticus.a membrane filtration procedure has been developed for the enumeration of vibrio parahaemolyticus in marine waters. background microbial growth on the primary medium was decreased through the use of sodium cholate and copper sulfate, high ph, 3% nacl, and an elevated incubation temperature. a series of in situ tests was employed to obviate the picking of colonies for identification; thereby, the enumeration of v. parahaemolyticus was accomplished within 30 h. confirmation of typical colonies app ...1976984837
food poisoning caused by parahaemolytic and nag vibrios after eating meat products.at total of 15 subjects taken ill with food poisoining caused by parahaemolytic and nag vibrios after eating boiled pork sausage and boiled veal stomach were examined. the meat products were secondarily contamined in the sausage factory due to the use od non-disinfected lake water which was found to be a natural medium of the vibrios. when the use of this water was prohibited, the contamination of the factory and the meat products disappeared and no further cases of food poisoning due to meat pr ...1976987098
vibrio parahaemolyticus: a cause of foodborne illness. 19761002591
[the spread of parahemolytic vibrios in humans in the burgass region (bulgaria)].the presence of parahemolytic vibrios in the water of the black sea and also in other water bodies, in the hydrobionts, and later in the secondarily contaminated meat products was demonstrated in 1968. in 1973 gastroenteritis caused by parahemolytic vibrios became widespread; 126 cases were confirmed bacteriologically, and 3 cases were found to be carriers. the infected food products were eliminated, and no more cases of the disease were revealed in 1974.19761007742
sublethal heat stress of vibrio parahaemolyticus.when vibrio parahaemolyticsu atcc 17802 was heated at 41 degrees c for 30 min in 100 mm phosphate-3% nacl buffer (ph 7.0), the plate counts obtained when using trypticase soy agar containing 0.25% added nacl (0.25 tsas) were nearly 99.9% higher than plate counts using trypticase soy agar containing 5.5% added nacl (5.5 tsas). a similar result was obtained when cells of v. parahaemolyticus were grown in a glucose salts medium (gsm) and heated at 45 degrees c. the injured cells recovered salt tole ...19761008556
vibrio parahaemolyticus gastroenteritis associated with international travel.food-borne gastroenteritis due to vibrio parahaemolyticus is becoming of increasing world importance. this paper describes the clinical presentations and laboratory investigations of four cases of gastroenteritis due to v. parahaemolyticus among passengers returning to australia on international aircraft from south-east asia.19761012128
studies of the cell envelope of vibrio para-haemolyticus a55: isolation and purification of bag-shaped peptidoglycan (murein sacculus).the peptidoglycan (pg) component of the envelope of vibrio parahaemolyticus a55 was studied in relation to the salt dependency of the organism. a bag-shaped pg (murein sacculus) was isolated and purified by digestion of freshly harvested intact whole cells with sodium dodecyl sulfate (sds) and then protease treatment. whole cells lyze in salt-deficient or hypotonic conditions and this can be measured by turbidometry. lysis is prevented by addition of 0.35 m nacl or acidification to below ph 4.5. ...19761016218
isolation of a small rod with lytic activity against vibrio parahaemolyticus from fresh sea water.a small rod, capable of formine crater-like plaques on lawns of vibrio parahaemolyticus, was isolated from a marine environment. the isolate was a gram-negative straight rod with round ends and was small in size, equal to that of halophilic bdellovibrio strain 5501. the isolate appeared to have close taxonomic relationships to cytophaga, since this bacterium moved slowly in a gliding manner on a solid agar surface, hydrolyzed agar and starch, contained yellow pigment and was halophilic. the isol ...19761018344
clinical and epidemiological studies on v. parahaemolyticus infection in calcutta (1975). 19761024059
sensitivity of vibrio group of organisms to minocycline--a new tetracycline derivative. 19761024085
comparative studies on the enzymes of different strains of vibrio cholerae & vibrio parahaemolyticus. 19761024973
investigations on the conservation of parahaemolytic vibrios by lyophilization and direct desiccation. (preliminary note). 19761030629
hydrogen sulphide production as an aid to the identification of vibrio parahaemolyticus.in this study 18 strains of vibrio parahaemolyticus from food and 8 from humans were tested for hydrogen sulphide production on various modifications of russel's triple sugar slopes and on tsi. all strains showed a characteristic surface browning on rts with andrade's indicator. this was not seen when rts with phenol red as indicator or tsi were used. appearance of this phenomenon allows unknown strains to be suspected as being vibrio parahaemolyticus.19761030841
chemical structure of the peptidoglycan of vibrio parahaemolyticus a55 with special reference to the extent of interpeptide cross-linking.the chemical structure of the cell wall peptidoglycan of vibrio parahaemolyticus a55 was studied. estimation of cross linkages between peptide subunits in the peptidoglycan by dinitrophenylation showed that about 30% of the total 2,6-diaminopimelic acid (a2pm) residues were involved in cross linkages. the presence of interpeptide bridges was also demonstrated by isolating bisdisaccharide peptide subunit dimers from chalaropsis muramidase digests of the cell wall peptidoglycan by gel filtration f ...19761030950
a bacterial cardiotoxin: thermostable direct hemolysin produced by vibrio parahaemolyticus. 19761031960
stopping of spontaneous beating of cultured mouse and rat myocardial cells by a toxin (thermostable direct hemolysin) from vibrio parahaemolyticus.low concentrations of thermostable direct hemolysin from vibrio parahaemolyticus stopped the spontaneous beating of cultured mouse and rat myocardial cells. these low concentrations depolarized the maximal diastolic potential and inhibited the generation of action potential of cultured myocardial cells. the toxin lost its activity when preincubated with ganglioside, gt1 or gm1. gt1 was more effective than gm1. high concentrations of the toxin caused morphological damage of cultured mouse and rat ...19761031961
increase in membrane conductance and positive inotropic action of hemolysin produced by vibrio parahaemolyticus on rabbit myocardium.the effects of purified thermostable direct hemolysin from the cultured filtrate of vibrio parahaemolyticus on both s-a node cells and atrial cells were studied electrophysiologically. hemolysin caused the membrane conductance to increase, thereby causing membrane depolarization. the major ion responsible for this depolarization was na+, but other ions, such as k+ and ca2+, also participated. positive inotropic action of hemolysin was observed, and the na-ca exchange mechanism was enhanced after ...19761031962
[presence and etio-epidemiological significance of halophilic vibriones in various material].in this work data of isolation of halophilic vibrios from coastal water of central tyrrhenian sea are reported, and the epidemiological significance of such strains is discussed. v. parahaemolyticus was identified in 8 out of 20 samples of sea water. v. alginolyticus was identified in 8 out of 26 samples of fishes and mussels examined and in 8 out of 20 sea water samples. halophilic vibrios had not been isolated from 12 samples of sewage water and from 27 healthy or pathological human stool samp ...1977901634
vibrio parahaemolyticus: aetiological agent of food poisoning.after mentioning the principal morphological, cultural, physiological, biochemical, enzyme and serological characteristics of vibrio parahaemolyticus (a facultative halophilic bacterium and the aetiological agent of food poisoning caused by the consumption of fish products) a description is given of its ecological aspects, geographical distribution, and potential enteropathogenicity for man, with general information on its isolation and identification.1976798718
[heterogenous patterns of pleiotropy in pts-mutants of vibrio parahaemolyticus]. 19761034032
human infections caused by v. parahaemolyticus in czechoslovakia.vibrio parahaemolyticus was isolated in two subjects with acute gastroenteritis. the patients' history included stay in pakistan or india. they probably contracted the infection on board an aeroplane where they ingested cold meal containing sea products. the possibility of this conditioned pathogen occurring also in an inland country is pointed out.19761034657
[induction and repression of l-arabinose isomerase in vibrio parahaemolyticus]. 19761034742
[defective utilization of various carbon sources in a mutant of vibrio parahaemolyticus lacking a component of the phosphoenolpyruvate: sugar phosphotransferace system]. 19761034743
elucidation of hydrocarbon structure in an enzyme-catalyzed benzo[a]pyrene-poly (g) covalent complex.the carcinogen, benzo[a]pyrene, was covalently attached to poly (g) by liver microsomes from rats pretreated with 3-methylcholanthrene. the complex was hydrolyzed with enzymes or base and products were isolated by sephadex chromatography. absorbance and fluorescence spectra of the products fit that of red-shifted pyrene aromatic system and suggest that metabolism has occurred at the 7-, 8-, 9-, and 10-positions of the hydrocarbon. benzanthracene or chrysene fluorescence were not observed in thes ...19761064016
vibrio parahaemolyticus-food poisoning: case report.symptoms of food poisoning occurred following the ingestion of raw shellfish purchased in the auckland area. vibrio parahaemolyticus was recoverer from the patient. the potential of this and closely related microorganisms to cause illness is reviewed.19761064793
evidence for glucocorticoid receptor in human leukocytes.the glucocorticoid uptake in vitro by human periferal leukocytes was studied. the uptake showed 2 main components, one saturable and one non-saturable. the saturable component was compared with the uptake by the specific glucocorticoid receptor in rabbit granulocytes. the similarities with the rabbit receptor in structural specificity, time course of uptake at 37 degrees c, sensitivity to metabolic inhibition by pcms and the physiological concentration for half saturation indicate that the satur ...19761068630
vibrio parahaemolyticus food poisoning: a case report. 19761072814
[the acute vestibular paralysis (author's transl)].acute vestibular paralysis may not be considered as a nosologic entity but as a syndrome. symptomatology (vertigo, spontaneous and provoked vestibular nystagmus, absence of cochlear signs) shows an uniform picture. the results of the caloric test as well as the nystagmic responses induced by galvanic stimulation and the development of central vestibular compensation however indicate that the site of the lesion is not only confined to the labyrinth but may also occur at the level of the periphera ...19751081389
polymorphism of human color vision.the genetic polymorphism of human color vision is examined within the framework of a photopigment replacement model. an analysis of the x-linked recessive dichromacies and anomalous trichromacies indicates that one source of variability of normal color perception may be the inclusion of several distinct phenotypes in what is usually described as normal color vision. this analysis also reveals the cause of the dominance hierarchy at the protan and deutan loci, the perceptual effects of dosage com ...19761087832
bacterial flora of the hemolymph of the blue crab, callinectes sapidus: numerical taxonomy.bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly vibrio sp., pseudomonas sp., acinetobacter sp., bacillus sp., flavobacterium sp., and coliforms. vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of may to november and was identified in up to 21% of the hemolymphs sampled.19751090258
macrophage-lymphocyte clustering in rheumatoid arthritis.the cells in synovial fluid from patients with rheumatoid arthritis contain a small percentage of macrophages. such macrophages were isolated and cultured alone and with homologous and heterologous lymphocytes for 24 hours, in an attempt to identify possible contact between living lymphocytes and macrophages. such contact was found, with clustering of lymphocytes around macrophages, and was particularly well shown by scanning electron microscopy.19751092274
bacteremia in suckling rabbits after oral challenge with vibrio parahaemolyticus.vibrio parahaemolyticus, a halophilic marine enteropathogen, produces diarrhea in man after ingestion of contaminated seafood. only strains capable of producing beta-hemolysis (kanagawa-positive strains) are enteropathogenic. yet the majority of marine isolants are nonhemolytic and kanagawa negative. studies were initiated in suckling rabbits in an attempt to elucidate pathogenetic mechanisms. fasting animals were infected orally with kanagawa-positive and kanagawa-negative strains of v. parahae ...19751095481
health hazards of bivalve-mollusk ingestion.bivalve mollusks (oysters, clans, and mussels) filter large quantities of water unselectively and thereby may concentrate a variety of aquatic contaminants pathogenic for man within edible shellfish viscera. the recognized bacterial disease associated with ingestion of contaminated bivalves include typhoid fever (not presently a public health problem), vibrio parahemolyticus gastroenteritis, and vibrio chloerae infection. the major known shellfish-associated viral diseases are viral hepatitis an ...19751096699
isolation and characterization of vibrio parahaemolyticus from cape cod soft-shell clams (mya arenaria).vibrio parahaemolyticus was isolated from soft-shell clams (mya arenaria) taken from 10 different clamming areas on cape cod, mass., during july and august 1972. direct plating on thiosulfate-citrate-bile salts-sucrose agar was found to be superior to either direct plating on vanderzant modified salt starch agar or enrichment with trypticase soy broth containing 7% salt for isolation from clam samples. morphological and biochemical characteristics of 33 isolates from 30 samples generally conform ...19751096817
proceedings: extraction, purification, and biophysico-chemical characteristics of a "kanagawa phenomenon"-associated hemolytic factor of vibrio parahaemolyticus. 19751099285
practical aspects of a cholera surveillance programme.the recent invasion of southern africa by cholera stimulated the establishment of a regional cholera reference laboratory and surveillance centre. this article describes its activities in general and details the methods employed in the isolation of vibrio cholerae from sewage, water, seafoods, patients, and carriers. importance is also attached to the isolation of nag (non-agglutinable) v. cholerae and v. parahaemolyticus from certain categories of specimens. in recognition of the importance of ...19751101396
vibrio parahaemolyticus gastroenteritis during, the el tor cholera epidemic in togo (west africa).in the course of the el tor cholera epidemic in togo (west africa), vibrio parahaemolyticus was identified as the causative agent of a cholera-like gastroenteritis. from september 1971 to march 1973 81 cases were confirmed bacteriologically. seventy patients developed cholera-like symptoms and at first were wrongly diagnosed as cholera cases; 6 other patients were simultaneoulsy infected with el tor vibrios and v. parachaemolyticus. there was a markedly higher incidence in males than in females. ...19751111349
isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of vibrio parahaemolyticus.a factor changing chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of vibrio parahaemolyticus. it was partially purified by successive column chromatographies on diethylaminoethylcellulose, diethylaminoethyl-sephadex a-25, hydroxylapatite, and sephadex g-200. this factor was separated from thermostabl direct hemolysin and was heat labile.19761033156
isolation of vibrio parahaemolyticus and vibrio alginolyticus from estuarine areas of southeastern alaska.the first reported isolations of halophilic vibrios, including vibrio parahaemolyticus, from three seafood processing areas in southeastern alaska are described.19751124924
isolation of vibrio parahaemolyticus from a knee wound.this is a case report of a contamination of an injured knee with an unusual organism, vibrio parahaemolyticus, that is likely to occur in patients living in coastal areas but often overlooked. the infection apppears to be sensitive to a number of antibiotics and very likely cured, whether recognized or not as the proper organism.19751126082
proceedings: cell-wall composition of aerobic cutaneous "diphtherios" organisms. 19751142405
cyanogen condensations as a route to 3-amino-2-imino-1,3-benzothiazin-4-ones with cns depressant potential.seven new 3-amino-2-imino-1,3-benzothiazin-4-ones, prepared from the condensation of cyanogen and 2-mercaptobenzhydrazides, were evaluated in a neuropharmacological mouse profile. cns depression was observed in several members of the class.19751151723
femoral percutaneous catheterization in infants and small children for cerebral angiography.an improved technique of selective cerebral angiography for infants and small chicken is described. the needle is inserted approximately 45 degrees to the skin in order to avoid subintimal placement of the tip. after good backflow is established, the guidewire is advanced to a position in the descending aorta. a no. 3 polyethylene catheter and 21-gauge scalp vein needle were most successful.19751153751
obesity induced by force-feeding and accompanying changes in body temperature and fertility in the male domestic fowl.1. excessive amounts of food (two-fold more than the ad libitum intake) introduced into the gastro-intestinal tract of three adult male fowls, for 5 weeks, caused a marked increase in body weight resulting mainly from fat deposition. 2. the induced obesity was accompanied by an increase of the rectal temperature (0-2 to 0-3 degrees c) and a decrease of the skin and comb temperatures, a cessation in the growth of the comb, a reduction in the blood packed cell volume, an eight-fold reduction in th ...19751156930
proceedings: effect of heat on direct hemolysin of vibrio parahaemolyticus; demonstration of an inactivating factor of purified thermostable direct hemolysin. 19751160199
[vibrio parahaemolyticus in mussels from the rijeka harbor]. 19751160477
vibrio parahaemolyticus -- a marine pathogen detected in south african coastal waters.vibrio parahaemolyticus has been detected in the natal and eastern cape costal water of south africa. as a proven cause of gastro-enteritis or a potential pathogen to humans, it is an organism of importance to public health. outlines of the history, pathogenicity and identification are given, as well as the results of the local investigation of this organism.19751162528
incidence of vibrio parahaemolyticus in chesapeake bay.a bay-wide survey of the distribution of vibrio parahaemolyticus was carried out in chesapeake bay during may 1972, to determine whether the annual cycle of v. parahaemolyticus which was observed to occur in the rhode river subestuary of chesapeake bay took place in other parts of chesapeake bay. in an earlier study, april to early june, when the water temperature rises from 14 to 19 c, was found to be a critical period in the annual cycle of the organism in the rhode river, since this is the ti ...19751164012
[editorial: vibrio parahaemolyticus]. 19751165079
characterization of the temperature-dependent inactivating factor of the thermostable direct hemolysin in vibrio parahaemolyticus.the termostable direct hemolysin of vibrio parahaemolyticus is inactivated by heating at around 55 c with an inactivating factor isolated from culture filtrates of v. parahaemolyticus. the characteristics of the temperature-dependent inactivating factor were studied by using polyacrylamide gel disc electrophoresis. it was found that when heated with the hemolysin, the inactivating factor destroyed the hemolysin and thus inactivated the hemolytic activity, suggesting that the inactivating factor ...19751165117
hl-a w27 in psoriatic arthropathy.forty subjects with psoriatic arthritis (pa) underwent hl-a tissue typing. hl-a w27 was significantly increased in psoriatic spondylitis (35%), whereas statistical significance was not reached in patients with peripheral arthritis alone, although w27 was present in a frequency greater than in controls. psoriatic spondylitis (often asymptomatic) occurred in 57% (23 of 40) of our pa patients. of interest, psoriatic spondylitis was more often w27 negative than w27 positive; nevertheless, w27-positi ...19751169069
the first reported case from panamá of acute gastroenteritis caused by vibrio parahaemolyticus.the first confirmed case of vibrio parahaemolyticus gastroenteritis in panama is reported. this marine organism, causing food poisoning in some countries and isolated only recently from seawater in panama, was recovered from the stools of a patient with acute gastroenteritis, as well as from fresh shrimp used in preparing seafood dishes. this person and 39 other individuals became acutely ill a few hours after eating shrimp at the same restaurant.19751171633
effect of increased static lung recoil on bronchial dimesions of excised lungs.we measured bronchial diameters and lengths during static deflation and inflation in eight excised dog lobes before and after static lung recoil (pst(l)) had been significantly increased by cooling the lobe for 48 h at 4 degrees c and ventilating it for 3 h. in control lobes, bronchial diameters were the same at any volume even though pst(l) was different during inflation and deflation. these results agree with those of hughes et al. (j. appl. physiol. 32: 25-35, 1972). however, when pst(l) was ...19751176409
vibrio parahaemolyticus as a cause of gastroenteritis in indonesia.vibrio parahaemolyticus was isolated from rectal swabs in two cases of acute gastroenteritis from different districts of jakarta.19751179488
[experimental basis of the method of correction of cerebrospinal hypertension in moderate hypothermia and occlusion of the main vessels of the heart]. 19751183385
cytotoxicity of vibrio parahaemolyticus in hela cell culture.in an investigation of the effect of vibrio parahaemolyticus on epithelial cells in vitro, hela cells in slide culture chambers were inoculated with washed bacteria and several bacterial preparations. preparations tested included live and heat-killed whole cells, unconcentrated culture filtrates, whole-cell lysates and their supernatant fluids, and supernatant fluids taken from hela cell monolayers previously inoculated with live bacteria. slide cultures were evaluated at 3 and 5 hr by light mic ...19751185016
inhibition of hemolytic activity of the thermostable direct hemolysin of vibrio parahaemolyticus by ganglioside.the hemolytic activity of the thermostable direct hemolysin produced by vibrio parahaemolyticus was inhibited by a ganglioside mixture. the ganglioside component which inhibited the hemolysin was not fm1 ganglioside.19751193720
brain 2-phenylethylamine as a major mediator for the central actions of amphetamine and methylphenidate. 19751196013
[isolation of parahemolytic vibrions from persons with acute gastrointestinal diseases].galophilic vibrios were for the first time in the soviet union isolated from the patients with gastrointestinal diseases in novorossiisk during the summer-autumn period of 1973. the greatest number of strains of the parahemolytic vibrios was isolated in august and september, during intensive fishing season, from the patients in whom the disease usually developed 6 to 12 hours after eating the sea fish. the parahemolytic vibrios isolated from the patients were typical by all signs and produced a ...19751199586
lethal effect of fresh sea water on vibrio parahaemolyticus and isolation of bdellovibrio parasitic against the organism.halophilic bdellovibrio, which is parasitic and lytic to vibrio pharahaemolyticus, was ioslated from fresh sea water in the winter. it had a lethal effect on v. parahaemolyticus. the optimum temperature ofr multiplication ranged from 25 c to 30 c and growth was not observed at 35 c. plaque numbers of the isolate reached a maximum in 17 hr under conditions of shaking at 25 c in autoclaved sea water supplemented with v. parahaemolyticus cells, and were as high as ten times the number of host cells ...19751202252
[experimental study of the action of some physical and chemical factors on the survival and propagation of vibrio parahaemolyticus in food products].the paper presents data on the ability of vibrio parahaemolyticus to survive and multiply under different conditions that may be encountered during storage and culinary treatment of food products. investigations were effected in a nutrient medium and in alimentary products in which the survival terms of vibrio parahaemolyticus. iv. occurrence of vibrio parahaemolyticus in sea mud and sea fish in hawaii. - secure an almost complete suppression of the vibrio parahaemolyticus viability. af greatest ...19751210207
[isolation of halphilic vibrios in marine fishes and fish products].examinations of 882 samples of newly caught, frozen, salted, sun-dried and smoked marine fish and of 50 samples of canned fish and preserves were conducted to study the contamination with halophil vibrios of the marine fish and fish products. the newly caught fish was found to be contaminated with vibrio parahaemolyticus on the average in 28.6 per cent of the cases and with vibrio alginolyitcus, on the average in 52.2 per cent of the instances. the vibrio parahaemolyticus contamination of the ma ...19751210210
[sensitivity of halophilic vibrions isolated on the black sea coast to some antibiotics and chemotherapeutic preparations]. 19751211889
[distribution of vibrio parahaemolyticus in various environmental objects]. 19751213453
serotypes of vibrio parahaemolyticus from clinical and environmental sources in togo (west africa).serological analysis of o and k antigens was performed on 343 strains of vibro parahaemolyticus isolated from clinical and environmental sources in togo. only two strains were not typable by the available o antisera. k untypable strains were found in 4.8% of isolates from gastroenteritis patients, in 11% from healthy carriers, and in 47% and 46% of isolates, respectively, from water and fish samples. thirteen serotypes identified in togo are not considered in the japanese antigenic scheme. the s ...19751214327
common morphogenetic aspects of various organotypic microvascular patterns. 19751214601
[the use of inorganic precursors of cysteine synthesis in the study of cysteine-deficient mutants of actinomyces olivaceus v kx]. 19751214652
[salmonellosis carrier state]. 19751214656
studies on vibrio parahaemolyticus infection in calcutta as compared to cholera infection. 19751219902
studies on vibrio parahaemolyticus in bombay. 19751219912
human infections caused by v. parahaemolyticus in czechoslovakia.vibrio parahaemolyticus was isolated in two subjects with acute gastroenteritis. the patients history included stay in pakistan or india. they probably contracted the infection on board an aeroplane where they ingested cold meal containing sea products. the possibility of this conditioned pathogen occurring also in an inland country is pointed out.19761033228
[the dehydrogenation of butandiol by "vibrio parahaemolyticus" and other vibrios (author's transl)].the butandiol dehydrogenase was examined in 64 vibrios isolated in togo 1970/71. this enzyme was found in all of the not halophilic strains and in the species vibrio alginolyticus, whereas the species v. parahaemolyticus exhibited as well dehydrating as not dehydrating strains. the butandiol dehydrogenase could be a good marker for epidemiologic analysis in characterizing v. parahaemolyticus strains isolated from different sources, especially if no antisera are available.1976952445
letter: vibrio parahaemolyticus septicemia. 19751111466
[surveys on the occurrence of vibrio parahaemolyticus on fish caught in the southern central baltic sea in comparison with other european results (author's transl)].presence of vibrio parahaemolyticus in european marine environments, including western baltic sea, was ascertained by several scientists. -our surveys had in view to find out whether fish of the southern central baltic sea area was contaminated with this microorganism. -in the periods of june - september 1973 and june 1974, 541 fish of 7 species had been tested. two different technics of isolation were applied. from among 820 colonies picked up for further identification none happened to be vibr ...19751220465
the in vitro activity of midecamycin against various vibrio species.the susceptibility of various vibrio species to midecamycin was studied by the tube dilution method. a mic of 100 mug per ml of midecamycin in bhi broth was taken as an indicator of bacterial resistance. all vibrio cholerae classic biotype strains tested, were sensitive to midecamycin. in respectively, 87.4%, 60.7% and 89.5% of vibrio cholerae el tor biotype, vibrio parahaemolyticus and vibrio nag strains tested, were sensitive to midecamycin.19751228226
proceedings: morphological changes of fl cells induced by thermostable direct hemolysin of vibrio parahaemolyticus. 19751228244
proceedings: neuraminidase-sensitive gangliosides: possible tissue receptors for thermostable direct hemolysin of vibrio parahaemolyticus. 19751228245
[changes of the inner ear in experimental stapedectomy]. 19751230953
[a cytophotometric and karyometric study of the granular cells of the rat brain. i. granular cells of the cochlear complex and cerebellum].two cell types can be distinguished in granular cells of the chochlear complex of the rat by the form of their processes and the structure of the nuclear chromatin. it is shown cytophotometrically that the majority of the granular cells of the cochlear complex and in the cerebellar cortex are diploid. however, some cells contain dna amount close to tetraploid. some correlation between the size of the nucleus and its dna amount was discovered both in the cerebellar cortex and in the cochlear comp ...19751231101
[new antigenic type k strains of vibrio parahaemolyticus isolated from food poisoning patients in kobe city]. 19751236972
[electron microscopic studies of epinephrine choroiditis in rabbits. 3. scanning electron microscopic observations of the retinal pigment epithelial cells in the early stage (author's transl)]. 19751239915
[dynamics of ribosomes of vibrio in the condition lacking salts]. 19751240190
[thermostable direct hemolysin of vibrio parahaemolyticus. (vi) its mechanism of action on fl cells]. 19751240261
[toxin produced by vibrio parahaemolyticus with lethal effects in mice]. 19751240262
[mechanism of arrhenius' effect of hemolysin produced by vibrio parahaemolyticus]. 19751240265
[flagella of enteritis-causing vibrio (v). conditions necessary for growth of the flagella]. 19751240320
changes in blood flow to bones during the hypocalcemic and hypercalcemic phases of the response to parathyroid hormone.labeled microspheres were used to measure blood flow to the leg bones of the laying hen at 0, 3, and 30 min after iv injection of parathyroid hormone (pth) (wilson) or the synthetic 1 to 34 fragment of bovine parathyroid hormone (pth 1-34). at 3 min, which corresponds to the hypocalcemic phase of the pth response, blood flow to the combined femur, tibia, and metatarus was significantly reduced by pth (wilson) relative to 0 time and to carrier-injected controls. at 30 min, i.e., the time of maxim ...19761248454
identification of lethal toxin with the thermostable direct hemolysin produced by vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.lethal toxin was purified extensively from the culture filtrate of a kanagawa phenomenon-positive strain of vibrio parahaemolyticus. the purified toxin was a protein, and its homogeneity was demonstrated by sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis and analytical ultracentrifugation. it was demonstrated that the thermostable direct hemolysin was identical to the lethal toxin and that it was the main, if not only, lethal toxin in the culture filtrate. the purified toxin had a ...19761248866
demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced by vibrio parahaemolyticus.intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by vibrio parahaemolyticus caused rapid death of rats. studies by electroencephalography and electrocardiography showed that after intravenous injection of the toxin the electroencephalogram remained normal for quite a long time after the heart of the animals had stopped beating. depression of intraatrial and intraventricular conduction of electrical activation, including atrioventricular block, was observed in el ...19761248868
the lecithin: cholesterol acyltransferase reaction, lysolecithin and red cell ageing in blood stored under normal transfusion service conditions.the lecithin:cholesterol acyltransferase (lcat) activity and lysolecithin content of human blood stored under standard blood transfusion service conditions at 4 degrees c for 6 weeks has been investigated. cooling the blood to 4 degrees c rapidly inactivates the lcat reaction, but the enzyme is not denatured during storage under these conditions. citrate in the anticoagulant did not activate the lcat reaction in freshly-taken whole blood. the total phospholipid and total lysolecithin content of ...19761251568
further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. ii. effect of cortisone acetate.studies from this laboratory have demonstrated that the administration of cyclophosphamide (cy) and corynebacterium parvum (cp) over a prolonged time to c3h mice with established measurable tumors resulted in complete arrest of tumor growth as well as partial and complete regressions in many instances. a study of the effect of two different doses of cortisone acetate (ca), administered two or five times weekly, on the tumor inhibitory properties of this chemoimmunotherapeutic regimen indicated t ...19761255788
the isolation of vibrio parahaemolyticus and related vibrios from moribund aquarium lobsters.vibrios were isolated in pure culture from the hemolymph of 7 out of 28 dead or dying aquarium lobsters which had been acclimated to 20-22 degrees c. one isolate was identified as vibrio parahaemolyticus, one as a related marine vibrio (probably v. marinus), and five as vibrio alginolyticus. no isolates of halophilic vibrio species were made from healthy lobsters using thiosulfate citrate bile salts sucrose agar (tcbs).19761260536
halophilic vibrio species isolated from blood cultures.the special bacteriology section of the center for disease control has received 38 cultures of a halophilic bacterium which apparently unnamed. on the basis of the minimal characteristics of vibrio species proposed by hugh and sakazaki, this bacterium belongs to the genus vibrio. the unnamed species can be differentiated from vibro parahaemolyticus by a lower tolerance for sodium chloride (nac1) and the fermentation of lactose. the failure to ferment sucrose is an additional characteristic which ...19761262454
the detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with dirofilaria immitis antigens.counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. antigens used in the test were prepared from microfilariae and adult male d. immitis. some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. the presence of soluble circulating ...19761262695
attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization.four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ...19761262709
false-positive rabbit ileal loop reactions attributed to vibrio parahaemolyticus broth filtrates.vibrio parahaemolyticus broth filtrates have previously been shown to produce positive reactions in rabbit ileal loops only if concentrated 10-fold by lyophilization. this method of concentration produces solutions that contain greater than 20% nacl. in the present study, however, concentrations of nacl of greater than or equal to 4% induced positive responses in ileal loops, and desalting rendered previously reactive, concentrated broth filtrates negative. therefore, enterotoxin was not demonst ...19761262710
cytotoxic effect of the thermostable direct hemolysin produced by vibrio parahaemolyticus on fl cells.the thermostable direct hemolysin produced by vibrio parahaemolyticus showed cytotoxic activity on fl cells derived from human amniotic membrane. scanning electron micrographs of the whole cells showed that the microvilli on the cell surface decreased in number and changed in shape on treatment with the hemolysin. most of the microvilli disappeared before death of the cells, as judged from the results of staining the cells with trypan blue and measuring release of alkaline phosphatase from the c ...19761270136
survey of media for the resuscitation of heat-stressed vibrio parahaemolyticus. 19761270363
colony morphology of vibrio cholerae on sv medium. 19761270369
morphological, cultural and biochemical characteristics of vibrio parahaemolyticus, isolated in romania from acute gastro-enteritis.out of 399 human faeces examined during the first eight months (1975) for the presence of ncv vibrios, one vibrio parahaemolyticus strains has been isolated from a man with acute gastro-enteritis (gastric and abdominal pains, nausea, diarrhoea, headache, general weakness), after having a meal with salted caviar. the strain belongs to heiberg's group vii and sakazaki's subgroup i. the virulence tested on chick embryo is 3 times higher (ld50 = 14 germs) if compared with the virulence of v. parahae ...19761274498
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