Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| epidemic clones, oceanic gene pools, and eco-ld in the free living marine pathogen vibrio parahaemolyticus. | we investigated global patterns of variation in 157 whole-genome sequences of vibrio parahaemolyticus, a free-living and seafood associated marine bacterium. pandemic clones, responsible for recent outbreaks of gastroenteritis in humans, have spread globally. however, there are oceanic gene pools, one located in the oceans surrounding asia and another in the mexican gulf. frequent recombination means that most isolates have acquired the genetic profile of their current location. we investigated ... | 2015 | 25605790 |
| identification of a serum amyloid a gene and the association of snps with vibrio-resistance and growth traits in the clam meretrix meretrix. | serum amyloid a (saa), an acute response protein as well as an apolipoprotein, is considered to play crucial roles in both innate immunity and lipid metabolism. in this study, a saa gene (mmsaa) was identified in the clam meretrix meretrix. the full length dna of mmsaa was 1407bp, consisting of three exons and two introns. the distribution of mmsaa in clam tissues was examined with the highest expression in hepatopancreas. in response to the vibrio parahaemolyticus challenge, mmsaa mrna showed s ... | 2015 | 25602707 |
| differential specificity of selective culture media for enumeration of pathogenic vibrios: advantages and limitations of multi-plating methods. | plating environmental samples on vibrio-selective chromogenic media is a commonly used technique that allows one to quickly estimate concentrations of putative vibrio pathogens or to isolate them for further study. although this approach is convenient, its usefulness depends directly on how well the procedure selects against false positives. we tested whether a chromogenic medium, chromagar vibrio (cav), used alone (single-plating) or in combination (double-plating) with a traditional medium thi ... | 2015 | 25602161 |
| identification and molecular characterization of a peritrophin-like gene, involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | peritrophin was first isolated from insect peritrophic membrane (pm) and was thought to protect insects from invasion of microorganisms and to stimulate digestion of food. in this study, a peritrophin-like gene (espt) was obtained from eriocheir sinensis. the full length cdna of espt was 1232 bp, which contained 1005 bp orf encoding a protein of 334 amino acids, including a 22 amino acid signal peptide, and 3 conserved chitin binding type 2 domains (chtbd2) characterized by having a 6-cysteine m ... | 2015 | 25596425 |
| complete genome sequence of vpkk5, a novel vibrio parahaemolyticus lytic siphophage. | this paper describes the complete sequence of a novel lytic marine siphophage, vpkk5, that is specific to vibrio parahemolyticus. | 2015 | 25573936 |
| vibrio parahaemolyticus strengthens their virulence through modulation of cellular reactive oxygen species in vitro. | vibrio parahaemolyticus (vp) is one of the emergent food-borne pathogens that are commensally associated with various shellfish species throughout the world. it is strictly environmental and many strains are pathogenic to humans. the virulent strains cause distinct diseases, including wound infections, septicemia, and most commonly, acute gastroenteritis, which is acquired through the consumption of raw or undercooked seafood, especially shellfish. vp has two type three secretion systems (t3sss) ... | 2014 | 25566508 |
| vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques. | vibrio parahaemolyticus is a gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. v. parahaemolyticus is the leading causal agent of human acute gastroenteritis following the consumption of raw, undercooked, or mishandled marine products. in rare cases, v. parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-existing medical conditions. v. parahaemolyticus has two hemolysins virulence factors that are thermostabl ... | 2014 | 25566219 |
| h-ns is a repressor of major virulence gene loci in vibrio parahaemolyticus. | vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci t3ss1, vp-pai (t3ss1+tdh2) and t6ss2. as showing in this study, the nucleoid-associated dna-binding regulator h-ns binds to multiple promoter-proximal regions in each of the above three loci to repress their transcription, and moreover h-ns inhibits the cytotoxicitiy, enterotoxicity, hemolytic activity, and mouse lethality of v. parahaemolyticus. h-ns appears to ac ... | 2014 | 25566201 |
| a c-type lectin (lvctl4) from litopenaeus vannamei is a downstream molecule of the nf-κb signaling pathway and participates in antibacterial immune response. | c-type lectins (ctls) play multiple roles in innate immune defense against invading pathogens in both vertebrates and invertebrates. in this study, a new c-type lectin gene from pacific white shrimp litopenaeus vannamei (designated as lvctl4) was cloned by rapid amplification of the cdna ends (race) method. the full-length cdna of lvctl4 was 563 bp with open reading frame (orf) of 471 bp encoding a polypeptide of 156 amino acids, including a putative signal sequence and a single c-type lectin-li ... | 2015 | 25559446 |
| bacteriostatic suppression in norway lobster (nephrops norvegicus) exposed to manganese or hypoxia under pressure of ocean acidification. | future ocean acidification (oa) and warming following climate change elicit pervasive stressors to the inhabitants of the sea. previous experimental exposure to oa for 16 weeks at ph levels predicted for 2100 has shown to result in serious immune suppression of the norway lobster, nephrops norvegicus. the lobsters are currently affected by stressors such as periodical hypoxia inducing high levels of bioavailable manganese (mn). here, we aimed to investigate possible effects of interactions betwe ... | 2015 | 25553539 |
| field and experimental evidence of vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease of cultured shrimp (litopenaeus vannamei) in northwestern mexico. | moribund shrimp affected by acute hepatopancreatic necrosis disease (ahpnd) from farms in northwestern mexico were sampled for bacteriological and histological analysis. bacterial isolates were molecularly identified as vibrio parahaemolyticus by the presence of the tlh gene. the tdh-negative, trh-negative, and tlh-positive v. parahaemolyticus strains were further characterized by repetitive extragenic palindromic element-pcr (rep-pcr), and primers ap1, ap2, ap3, and ap and an ems2 iq2000 detect ... | 2015 | 25548045 |
| identification and function of 11 rab gtpases in giant freshwater prawn macrobrachium rosenbergii. | rab gtpases, members of the ras-like gtpase superfamily, are central elements in endocytic membrane trafficking. however, little is known of the rab genes in the giant freshwater prawn macrobrachium rosenbergii. in this study, 11 rab genes were identified from m. rosenbergii. all mrrabs have a rab domain. phylogenetic analysis showed that these 11 mrrabs were divided into different groups. the mrrab genes were ubiquitously expressed in heart, hemocytes, hepatopancreas, gills, stomach, and intest ... | 2015 | 25542378 |
| novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing. | two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [qs]). the basic design of these escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of qs signal molecules. consequently, these e. coli strains are unable to grow in the presence of the respective qs signal molecules unless a nontoxic qs-interfering compound is present. the first reporter strain designed to ... | 2015 | 25527543 |
| identification and functional analysis of a hemolin like protein from litopenaeus vannamei. | hemolin is a specific immune protein belonging to immunoglobulin superfamily and firstly identified in insects. growing evidences suggest that hemolin can be activated by bacterial and viral infections and may play an important role in antimicrobial immunity. in this paper, we firstly identified a hemolin-like protein from litopenaeus vannamei (lvhemolin). sequence analysis showed that lvhemolin shares high similarity with insect hemolins and is mainly composed of seven immunoglobulin (ig) domai ... | 2015 | 25527138 |
| metadynamics simulations reveal a na+ independent exiting path of galactose for the inward-facing conformation of vsglt. | sodium-galactose transporter (sglt) is a secondary active symporter which accumulates sugars into cells by using the electrochemical gradient of na+ across the membrane. previous computational studies provided insights into the release process of the two ligands (galactose and sodium ion) into the cytoplasm from the inward-facing conformation of vibrio parahaemolyticus sodium/galactose transporter (vsglt). several aspects of the transport mechanism of this symporter remain to be clarified: (i) a ... | 2014 | 25522004 |
| comparative genomic analysis of clinical and environmental strains provides insight into the pathogenicity and evolution of vibrio parahaemolyticus. | vibrio parahaemolyticus is a gram-negative halophilic bacterium. infections with the bacterium could become systemic and can be life-threatening to immunocompromised individuals. genome sequences of a few clinical isolates of v. parahaemolyticus are currently available, but the genome dynamics across the species and virulence potential of environmental strains on a genome-scale have not been described before. | 2014 | 25518728 |
| role of vcrd1 protein in expression and secretion of flagellar components in vibrio parahaemolyticus. | vcrd1 protein is a component of type iii secretion system (t3ss) 1 in vibrio parahaemolyticus. a comparative analysis of secretomes of wild-type and δvcrd1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. western blot analyses using specific antibodies confirmed that the secretion of at least four flagellar components, such as flaa, flgl, flge, and flgm, was affected by the vcrd1 mutation, which was consistent with decreased ... | 2015 | 25516430 |
| environmental parameters influence on the dynamics of total and pathogenic vibrio parahaemolyticus densities in crassostrea virginica harvested from mexico's gulf coast. | the influence of environmental parameters on the total and pathogenic vibrio parahaemolyticus seasonal densities in american oysters (crassostrea virginica) was evaluated for 1 year. harvesting site a yielded the highest mean densities of v. parahaemolyticus tlh+, tdh+/trh-, tdh-/trh+ and tdh+/trh+ during spring season at 2.57, 1.74, 0.36, and -0.40 log10 mpn/g, respectively, and tdh+/orf8+ during winter season (0.90 log10 mpn/g). v. parahaemolyticus tlh+ densities were associated to salinity (r ... | 2015 | 25510545 |
| molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab scylla paramamosain. | the thioredoxin (trx) system consists of thioredoxin reductase (trxr), trx, and nicotinamide adenine dinucleotide phosphate (nadph). trxr is an nadph-dependent oxidoreductase. trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. in this study, a cdna sequence (sptrx1) showing high identity to the first trx gene was isolated from a hepatopancreas cdna library of the mud crab scylla paramamosain. the full-le ... | 2014 | 25501236 |
| efficacy of low-temperature high hydrostatic pressure processing in inactivating vibrio parahaemolyticus in culture suspension and oyster homogenate. | culture suspensions of five clinical and five environmental vibrio parahaemolyticus strains in 2% nacl solution were subjected to high pressure processing (hpp) under various conditions (200-300mpa for 5 and 10 min at 1.5-20°c) to study differences in pressure resistance among the strains. the most pressure-resistant and pressure-sensitive strains were selected to investigate the effects of low temperatures (15, 5 and 1.5°c) on hpp (200 or 250mpa for 5 min) to inactivate v. parahaemolyticus in s ... | 2015 | 25498471 |
| regulation of vibrio parahaemolyticus t3ss2 gene expression and function of t3ss2 effectors that modulate actin cytoskeleton. | vibrio parahaemolyticus is a leading causative agent of seafood-borne gastroenteritis worldwide. most clinical isolates from patients with diarrhoea possess two sets of genes for the type iii secretion system (t3ss) on each chromosome (t3ss1 and t3ss2). t3ss is a protein secretion system that delivers effector proteins directly into eukaryotic cells. the injected effectors modify the normal cell functions by altering or disrupting the normal cell signalling pathways. of the two sets of t3ss gene ... | 2015 | 25495647 |
| insights into vibrio parahaemolyticus chn25 response to artificial gastric fluid stress by transcriptomic analysis. | vibrio parahaemolyticus is the causative agent of food-borne gastroenteritis disease. once consumed, human acid gastric fluid is perhaps one of the most important environmental stresses imposed on the bacterium. herein, for the first time, we investigated vibrio parahaemolyticus chn25 response to artificial gastric fluid (agf) stress by transcriptomic analysis. the bacterium at logarithmic growth phase (lgp) displayed lower survival rates than that at stationary growth phase (sgp) under a sub-le ... | 2014 | 25490137 |
| transcriptome and expression profiling analysis of the hemocytes reveals a large number of immune-related genes in mud crab scylla paramamosain during vibrio parahaemolyticus infection. | mud crab scylla paramamosain is an economically important marine species in china. however, frequent outbreaks of infectious diseases caused by marine bacteria, such as vibrio parahaemolyticus, result in great economic losses. | 2014 | 25486443 |
| structure-based analysis of domain function of chitin oligosaccharide deacetylase from vibrio parahaemolyticus. | the x-ray crystal structure of chitin oligosaccharide deacetylase from vibrio parahaemolyticus (vp-cod) was determined at an 1.35 å resolution. the amino acid sequence and structure of vp-cod show that the enzyme comprises one polysaccharide deacetylase domain (pdd) and two carbohydrate-binding domains (cbds). on the basis of a chitin-binding assay with vp-cod and its cbds-deleted mutant, it was confirmed that cbds can adhere to chitin. the catalytic activity of the cbds-deleted mutant was only ... | 2015 | 25479092 |
| depuration times of donax trunculus and tapes decussatus. | the present study was performed to determine the depuration time and ability of donax trunculus (wedge clam) and tapes decussatus (carpet shell) contaminated with escherichia coli, salmonella enterica subsp. enterica serovar typhimurium and vibrio parahaemolyticus. clams were contaminated with each bacterium at the level of 7.0 - 8.0 log10 cfu/g. after contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. during the depuration process of bo ... | 2014 | 25477938 |
| function of a novel c-type lectin with two crd domains from macrobrachium rosenbergii in innate immunity. | c-type lectins play crucial roles in innate immunity. in the present study, a novel c-type lectin gene, designated as mrctl, was identified from macrobrachium rosenbergii. mrctl contains 2 carbohydrate-recognition domains (crds), namely mrcrd1 and mrcrd2. the mrcrd1 contains a qep motif and mrcrd2 contains a motif of epd. mrctl was mainly expressed in the hepatopancreas. the expression level of mrctl in hepatopancreas was significantly upregulated after a challenge with vibrio parahaemolyticus o ... | 2015 | 25475962 |
| efficacy of neutral electrolyzed water for reducing pathogenic bacteria contaminating shrimp. | pathogenic contamination is a food safety concern. this study was conducted to investigate the efficacy of neutral electrolyzed water (new) in killing pathogens, namely, vibrio parahaemolyticus, vibrio vulnificus, salmonella enteritidis, and escherichia coli in shrimp. pure cultures of each pathogen were submerged separately in new containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. for each concentration, three submersion times were tested: 1, 3, and 5 min. the popul ... | 2014 | 25474069 |
| microbiological quality of seasoned roasted laver and potential hazard control in a real processing line. | microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ... | 2014 | 25474052 |
| intervention strategies for reducing vibrio parahaemolyticus in seafood: a review. | vibrio parahaeomolyticus, a natural inhabitant in estuarine marine water, has been frequently isolated from seafood. it has been recognized as the leading causative agent for seafoodborne illness all over the world. numerous physical, chemical, and biological intervention methods for reducing v. parahaeomolyticus in seafood products have been investigated and practiced. each intervention method has distinct advantages and disadvantages depending on the processing needs and consumer preference. t ... | 2015 | 25472618 |
| simultaneous construction of pcr-dgge-based predictive models of listeria monocytogenes and vibrio parahaemolyticus on cooked shrimps. | the aim of this study was to simultaneously construct pcr-dgge-based predictive models of listeria monocytogenes and vibrio parahaemolyticus on cooked shrimps at 4 and 10°c. calibration curves were established to correlate peak density of dgge bands with microbial counts. microbial counts derived from pcr-dgge and plate methods were fitted by baranyi model to obtain molecular and traditional predictive models. for l. monocytogenes, growing at 4 and 10°c, molecular predictive models were construc ... | 2015 | 25470339 |
| molecular characterization and functional analysis of cactin gene from litopenaeus vannamei. | cactin (cactus interactor) is a conserved protein which was initially discovered as a novel interactor of drosophila ikb protein cactus. cactin was always characterized as a negative regulator of many different developmental processes, but only found to play an immune role in humans. to better know the immune function of cactin gene, litopenaeus vannamei cactin (lvcactin) was identified and characterized in this study. the full-length cdna of lvcactin is 3, 150 bp long, with an open reading fram ... | 2014 | 25462455 |
| association of crispr/cas evolution with vibrio parahaemolyticus virulence factors and genotypes. | clustered regularly interspaced short palindromic repeats (crispr), which is considered to be an immune system for bacteria, has been widely used as a tool for genome editing and genotyping. it has also been reported to be associated with virulence factors in some bacteria. to understand the role of crispr in the virulence and evolution of pathogenic vibrio parahaemolyticus, 154 v. parahaemolyticus strains isolated from clinical samples and 54 strains from food samples taken in shenzhen, china w ... | 2015 | 25455966 |
| recreational swimmers' exposure to vibrio vulnificus and vibrio parahaemolyticus in the chesapeake bay, maryland, usa. | vibrio vulnificus and vibrio parahaemolyticus are ubiquitous in the marine-estuarine environment, but the magnitude of human non-ingestion exposure to these waterborne pathogens is largely unknown. we evaluated the magnitude of dermal exposure to v. vulnificus and v. parahaemolyticus among swimmers recreating in vibrio-populated waters by conducting swim studies at four swimming locations in the chesapeake bay in 2009 and 2011. volunteers (n=31) swam for set time periods, and surface water (n=25 ... | 2015 | 25454225 |
| vibrio effector protein vopq inhibits fusion of v-atpase-containing membranes. | vesicle fusion governs many important biological processes, and imbalances in the regulation of membrane fusion can lead to a variety of diseases such as diabetes and neurological disorders. here we show that the vibrio parahaemolyticus effector protein vopq is a potent inhibitor of membrane fusion based on an in vitro yeast vacuole fusion model. previously, we demonstrated that vopq binds to the v(o) domain of the conserved v-type h(+)-atpase (v-atpase) found on acidic compartments such as the ... | 2015 | 25453092 |
| development of a matrix tool for the prediction of vibrio species in oysters harvested from north carolina. | the united states has federal regulations in place to reduce the risk of seafood-related infection caused by the estuarine bacteria vibrio vulnificus and vibrio parahaemolyticus. however, data to support the development of regulations have been generated in a very few specific regions of the nation. more regionally specific data are needed to further understand the dynamics of human infection relating to shellfish-harvesting conditions in other areas. in this study, oysters and water were collec ... | 2015 | 25452288 |
| flightless-i (flii) is a potential negative regulator of the toll pathway in litopenaeus vannamei. | flightless-i (flii) is a protein negatively modulates the toll-like receptor (tlr) pathway through interacting with myeloid differentiation factor 88 (myd88). to investigate the function of flii in innate immune responses in invertebrates, litopenaeus vannamei flii (lvflii) was identified and characterized. the full-length cdna of lvflii is 4, 304 bp long, with an open reading frame (orf) encoding a putative protein of 1292 amino acids, including 12 leucine-rich repeat (lrr) domains at the n-ter ... | 2015 | 25449702 |
| n-hexanoyl-l-homoserine lactone-degrading pseudomonas aeruginosa psdahp1 protects zebrafish against vibrio parahaemolyticus infection. | four strains of n-hexanoyl-l-homoserine lactone (ahl)-degrading pseudomonas spp., named psdahp1, psdahp2, psdahp3, and psdahp4 were isolated and identified from the intestine of fenneropenaeus indicus. psdahp1 showed the highest ahl-degrading activity among the four isolates. psdahp1 inhibited biofilm-forming exopolysaccharide and altered cell surface hydrophobicity of virulent green fluorescent protein (gfp)-tagged vibrio parahaemolyticus dahv2 (gfp-vpdahv2). oral administration of psdahp1 sign ... | 2015 | 25449384 |
| the role of cu/zn-sod and mn-sod in the immune response to oxidative stress and pathogen challenge in the clam meretrix meretrix. | the copper/zinc superoxide dismutase (cu/zn-sod) and manganese superoxide dismutase (mn-sod) could effectively eliminate reactive oxygen species (ros) and maintain the redox balance of immune system. in the present study, the potential synergy of cu/zn-sod and mn-sod in immune system was investigated in the clam meretrix meretrix. the expression of cu/zn-sod mainly distributed in hepatopancreas and that of mn-sod was higher in gill of m. meretrix, and their mrna and protein activity paralleled w ... | 2015 | 25449371 |
| prevalence and quantification of pathogenic vibrio parahaemolyticus during shrimp culture in thailand. | vibrio parahaemolyticus is a major cause of seafood-borne gastroenteritis. the human pathogenic strains possess tdh or trh or both genes. in thai shrimp farming, the level of pathogenic v. parahaemolyticus contamination has not been completely characterized, although it has been identified as a risk for people who consume undercooked shrimp. in this study, the prevalence and concentration of v. parahaemolyticus (total vp) and pathogenic v. parahaemolyticus (tdh+ vp and trh+ vp) were investigated ... | 2014 | 25449321 |
| genetic variation in vibrio parahaemolyticus isolated from the aquacultural environments. | environmental strains of vibrio parahaemolyticus, a foodborne pathogen, were isolated from milkfish and grouper aquaculture facilities in southern taiwan and characterized by pulsed-field gel electrophoresis (pfge) and polymerase chain reaction targeting on the virulence-associated and genomic island (vpai) marker genes. analyses of 62 environmental isolates, including two putative pathogenic isolates, by noti-pfge revealed 11 pulse-type clusters with a similarity of 85%. some of the t3ss2α-asso ... | 2015 | 25442717 |
| subversion of the cytoskeleton by intracellular bacteria: lessons from listeria, salmonella and vibrio. | entry into host cells and intracellular persistence by invasive bacteria are tightly coupled to the ability of the bacterium to disrupt the eukaryotic cytoskeletal machinery. herein we review the main strategies used by three intracellular pathogens to harness key modulators of the cytoskeleton. two of these bacteria, namely listeria monocytogenes and salmonella enterica serovar typhimurium, exhibit quite distinct intracellular lifestyles and therefore provide a comprehensive panel for the under ... | 2015 | 25440316 |
| antibiotic activity and microbial community of the temperate sponge, haliclona sp. | sessile marine invertebrates engage in a diverse array of beneficial interactions with bacterial symbionts. one feature of some of these relationships is the presence of bioactive natural products that can defend the holobiont from predation, competition or disease. in this study, we investigated the antimicrobial activity and microbial community of a common temperate sponge from coastal north carolina. | 2015 | 25431341 |
| identification of a second substrate-binding site in solute-sodium symporters. | the structure of the sodium/galactose transporter (vsglt), a solute-sodium symporter (sss) from vibrio parahaemolyticus, shares a common structural fold with leut of the neurotransmitter-sodium symporter family. structural alignments between leut and vsglt reveal that the crystallographically identified galactose-binding site in vsglt is located in a more extracellular location relative to the central substrate-binding site (s1) in leut. our computational analyses suggest the existence of an add ... | 2015 | 25398883 |
| distribution and growth of vibrio parahaemolyticus in southern chilean clams (venus antiqua) and blue mussels (mytilus chilensis). | we evaluated the distribution and growth of vibrio parahaemolyticus in the inland sea of southern chile, where the world's largest foodborne gastroenteritis outbreak by the pandemic strain o3:k6 occurred in 2005. intertidal samples of mytilus chilensis and venus antiqua were collected around port towns between 41°28's and 43°07's, during april to may 2011 and january to march 2012. we used most probable number real-time polymerase chain reaction (mpn-pcr) for enumeration of the tlh, tdh, and trh ... | 2015 | 25383987 |
| risk factors for sporadic vibrio parahaemolyticus gastroenteritis in east china: a matched case-control study - corrigendum. | 2015 | 25345865 | |
| [purification and dna-binding of toxr truncated protein of vibrio parahaemolyticus]. | the dna-binding domain of toxr protein of vibrio parahaemolyticus was expressed using the escherichia coli bl21lambdade3 protein expression system, and its dna-binding activity was characterized. | 2014 | 25345029 |
| [metabolome response to tdh expression of pathogenic vibrio parahaemolyticus induced by different temperatures]. | we studied the metabolome responses to different relative virulence gene expression of pathogenic vibrio parahaemolyticus based on reverse transcription real-time pcr (rt-pcr) and metabolomics. | 2014 | 25345019 |
| deciphering the role of multiple betaine-carnitine-choline transporters in the halophile vibrio parahaemolyticus. | vibrio parahaemolyticus is a halophile that is the predominant cause of bacterial seafood-related gastroenteritis worldwide. to survive in the marine environment, v. parahaemolyticus must have adaptive strategies to cope with salinity changes. six putative compatible solute (cs) transport systems were previously predicted from the genome sequence of v. parahaemolyticus rimd2210633. in this study, we determined the role of the four putative betaine-carnitine-choline transporter (bcct) homologues ... | 2015 | 25344241 |
| detection and identification of six foodborne bacteria by two-tube multiplex real time pcr and melting curve analysis. | this study is aimed to develop a two-tube melting curve-based multiplex real time pcr assay (mcmrt-pcr) for the simultaneous detection of six common foodborne pathogenic bacteria (diarrhoeagenic escherichia coli, salmonella, and shigella in tube 1, staphylococcus aureus, vibrio parahaemolyticus, and listeria monocytogenes in tube 2). | 2014 | 25341812 |
| generation and characterization of a scfv antibody against t3ss needle of vibrio parahaemolyticus. | vibrio parahaemolyticus, a halophilic gram-negative bacterium, is a food-borne pathogen that largely inhabits marine and estuarine environments, and poses a serious threat to human and animal health all over the world. the hollow "needle" channel, a specific assemble of t3ss which exists in most of gram-negative bacteria, plays a key role in the transition of virulence effectors to host cells. in this study, needle protein vp1694 was successfully expressed and purified, and the fusion protein tr ... | 2014 | 25320414 |
| temperature effect on high salinity depuration of vibrio vulnificus and v. parahaemolyticus from the eastern oyster (crassostrea virginica). | vibrio vulnificus (vv) and vibrio parahaemolyticus (vp) are opportunistic human pathogens naturally associated with the eastern oyster crassostrea virginica. the abundances of both pathogens in oysters are positively correlated with temperature, thus ingestion of raw oysters during the warm summer months is a risk factor for contracting illness from these bacteria. current post-harvest processing (php) methods for elimination of these pathogens are expensive and kill the oyster, changing their o ... | 2015 | 25310264 |
| immunological function of familial mediterranean fever disease protein pyrin. | pyrin, encoded by mefv gene, is conserved in humans and mice. mutations in the mefv gene are associated with the human autoinflammatory disease familial mediterranean fever (fmf). pyrin can interact with the inflammasome adaptor asc and induce inflammatory caspase-1 activation in monocytic cells, but the physiological function of pyrin has been unknown for many years. here we summarize previous studies of pyrin function under the context of fmf and immunity, and discuss a recent study demonstrat ... | 2014 | 25307949 |
| ampylation of rho gtpases subverts multiple host signaling processes. | rho gtpases are frequent targets of virulence factors as they are keystone signaling molecules. herein, we demonstrate that ampylation of rho gtpases by vops is a multifaceted virulence mechanism that counters several host immunity strategies. activation of nfκb, erk, and jnk kinase signaling pathways were inhibited in a vops-dependent manner during infection with vibrio parahaemolyticus. phosphorylation and degradation of ikbα were inhibited in the presence of vops as was nuclear translocation ... | 2014 | 25301945 |
| structural and regulatory mutations in vibrio parahaemolyticus type iii secretion systems display variable effects on virulence. | the gram-negative bacterium, vibrio parahaemolyticus, is a major cause of seafood-derived food poisoning throughout the world. the pathogenicity of v. parahaemolyticus is attributed to several virulence factors, including two type iii secretion systems (t3ss), t3ss1 and t3ss2. herein, we compare the virulence of v. parahaemolyticus por strains, which harbor a mutation in the t3ss needle apparatus of either system, to v. parahaemolyticus cab strains, which harbor mutations in positive transcripti ... | 2014 | 25288215 |
| characterization of vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in southern thailand. | vibrio parahaemolyticus was isolated from shrimp of five farms located in the pattani and songkhla provinces of southern thailand. using a pcr method targeted to the unique dna sequences derived from the plasmid (ap2 primers) and the toxin gene (ap3 primers) of v. parahaemolyticus that caused acute hepatopancreatic necrosis disease (ahpnd), a total of 33 of 108 isolates were positive. in contrast, all 63 and 66 isolates of clinical and environmental v. parahaemolyticus, respectively, obtained pr ... | 2015 | 25287127 |
| [o3:k6 serogroup vibrio parahaemolyticus - the causative agent of food toxic infection outbreaks in primorsky region of russian federation]. | comparative evaluation of biological properties of parahemolytic vibrios that had determined outbreaks and sporadic cases of food toxic infection in primorsky region in 2012 and previous years. materials and methods: 40 clinical strains of vibrio parahaemolyticus isolated in 2012 were studied in comparison with 62 strains from this region that had been characterized by us previously. virulence was evaluated by a complex method: hemolytic activitywas determined in kanagawa test (kt), urease - in ... | 2014 | 25286530 |
| occurrence of vibrio vulnificus and toxigenic vibrio parahaemolyticus on sea catfishes from galveston bay, texas. | dorsal and pectoral fin spines from two species of sea catfishes (bagre marinus and ariopsis felis) landed at 54 sites in galveston bay, texas, and its sub-bays from june to october 2005 were screened with traditional cultivation-based assays and quantitative pcr assays for vibrio vulnificus and vibrio parahaemolyticus. v. vulnificus was present on 51.2% of fish (n = 247), with an average of 403 ± 337 sd cells g(-1). v. parahaemolyticus was present on 94.2% (n = 247); 12.8% tested positive for t ... | 2014 | 25285498 |
| abundance of vibrio cholerae, v. vulnificus, and v. parahaemolyticus in oysters (crassostrea virginica) and clams (mercenaria mercenaria) from long island sound. | vibriosis is a leading cause of seafood-associated morbidity and mortality in the united states. typically associated with consumption of raw or undercooked oysters, vibriosis associated with clam consumption is increasingly being reported. however, little is known about the prevalence of vibrio spp. in clams. the objective of this study was to compare the levels of vibrio cholerae, vibrio vulnificus, and vibrio parahaemolyticus in oysters and clams harvested concurrently from long island sound ... | 2014 | 25281373 |
| homologues of insecticidal toxin complex genes within a genomic island in the marine bacterium vibrio parahaemolyticus. | three insecticidal toxin complex (tc)-like genes were identified in vibrio parahaemolyticus 13-028/a3, which can cause acute hepatopancreatic necrosis disease in penaeid shrimp. the three genes are a tcda-like gene (7710 bp), predicted to code for a 284-kda protein; a tcdb-like gene (4272 bp), predicted to code for a 158-kda protein; and a tccc3-like gene (2916 bp), predicted to encode a 107-kda protein. all three predicted proteins contain conserved domains that are characteristic of their resp ... | 2014 | 25272969 |
| toxicological responses of the hard clam meretrix meretrix exposed to excess dissolved iron or challenged by vibrio parahaemolyticus. | the responses of genes encoding defense components such as ferritin, the lipopolysaccharide-induced tumor necrosis factor-alpha factor (litaf), the inhibitor of nuclear factor-κb (iκb), metallothionein, and glutathione peroxidase were assessed at the transcriptional level in order to investigate the toxicological and immune mechanism of the hard clam meretrix meretrix (hcmm) following challenge with iron or a bacterium (vibrio parahaemolyticus). fe dissolved in natural seawater led to an increas ... | 2014 | 25269138 |
| comparative rna-seq based dissection of the regulatory networks and environmental stimuli underlying vibrio parahaemolyticus gene expression during infection. | vibrio parahaemolyticus is the leading worldwide cause of seafood-associated gastroenteritis, yet little is known regarding its intraintestinal gene expression or physiology. to date, in vivo analyses have focused on identification and characterization of virulence factors--e.g. a crucial type iii secretion system (t3ss2)--rather than genome-wide analyses of in vivo biology. here, we used rna-seq to profile v. parahaemolyticus gene expression in infected infant rabbits, which mimic human infecti ... | 2014 | 25262354 |
| multivalent adhesion molecule 7 clusters act as signaling platform for host cellular gtpase activation and facilitate epithelial barrier dysfunction. | vibrio parahaemolyticus is an emerging bacterial pathogen which colonizes the gastrointestinal tract and can cause severe enteritis and bacteraemia. during infection, v. parahaemolyticus primarily attaches to the small intestine, where it causes extensive tissue damage and compromises epithelial barrier integrity. we have previously described that multivalent adhesion molecule (mam) 7 contributes to initial attachment of v. parahaemolyticus to epithelial cells. here we show that the bacterial ad ... | 2014 | 25255250 |
| identification of capsule, biofilm, lateral flagellum, and type iv pili in vibrio mimicus strains. | vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. it is distributed worldwide. factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. in pathogenic vibrio species such as v. cholerae, vibrio. parahaemolyticus and vibrio vulnificu ... | 2014 | 25246027 |
| activities of alkyl hydroperoxide reductase subunits c1 and c2 of vibrio parahaemolyticus against different peroxides. | alkyl hydroperoxide reductase subunit c gene (ahpc) functions were characterized in vibrio parahaemolyticus, a commonly occurring marine food-borne enteropathogenic bacterium. two ahpc genes, ahpc1 (vpa1683) and ahpc2 (vp0580), encoded putative two-cysteine peroxiredoxins, which are highly similar to the homologous proteins of vibrio vulnificus. the responses of deletion mutants of ahpc genes to various peroxides were compared with and without gene complementation and at different incubation tem ... | 2014 | 25239899 |
| identification of a novel vaccine candidate by immunogenic screening of vibrio parahaemolyticus outer membrane proteins. | vibrio parahaemolyticus is an important halophilous pathogen that can cause not only a broad range of disease in aquatic animals but also serious seafood-borne illness in humans as a result of the consumption of seafood. to avoid the use of antibiotics, it is critical to identify protective antigens for developing highly effective vaccines against this pathogen. outer membrane proteins (omps) have been suggested as potential vaccine candidates for conferring protection against infection. in this ... | 2014 | 25236587 |
| population structure of clinical vibrio parahaemolyticus from 17 coastal countries, determined through multilocus sequence analysis. | vibrio parahaemolyticus is a leading cause of food-borne gastroenteritis worldwide. although this bacterium has been the subject of much research, the population structure of clinical strains from worldwide collections remains largely undescribed, and the recorded outbreaks of v. parahaemolyticus gastroenteritis highlight the need for the subtyping of this species. we present a broad phylogenetic analysis of 490 clinical v. parahaemolyticus isolates from 17 coastal countries through multilocus s ... | 2014 | 25225911 |
| a novel, broad-range, ctxφ-derived stable integrative expression vector for functional studies. | ctxφ, a filamentous vibriophage encoding cholera toxin, uses a unique strategy for its lysogeny. the single-stranded phage genome forms intramolecular base-pairing interactions between two inversely oriented xerc and xerd binding sites (xbs) and generates a functional phage attachment site, attp(+), for integration. the attp(+) structure is recognized by the host-encoded tyrosine recombinases xerc and xerd (xercd), which enables irreversible integration of ctxφ into the chromosome dimer resoluti ... | 2014 | 25225263 |
| intracellular vibrio parahaemolyticus escapes the vacuole and establishes a replicative niche in the cytosol of epithelial cells. | vibrio parahaemolyticus is a globally disseminated gram-negative marine bacterium and the leading cause of seafood-borne acute gastroenteritis. pathogenic bacterial isolates encode two type iii secretion systems (t3ss), with the second system (t3ss2) considered the main virulence factor in mammalian hosts. for many decades, v. parahaemolyticus has been studied as an exclusively extracellular bacterium. however, the recent characterization of the t3ss2 effector protein vopc has suggested that thi ... | 2014 | 25205094 |
| enzymatic elucidation of haemocyanin from kuruma shrimp marsupenaeus japonicus and its molecular recognition mechanism towards pathogens. | haemocyanin is an important non-specific immune protein present in the hemolymph of invertebrates, which have the ability to recognize the microbial pathogens and trigger the innate immune system. in this study, we isolated and purified the haemocyanin using gel filtration chromatography and investigated its microbial recognition mechanism against the invading pathogens. kuruma shrimp marsupenaeus japonicus haemocyanin showed the single band with a molecular weight of 76 kda on sds-page and its ... | 2015 | 25204648 |
| [apha is an activator of c-di-gmp synthesis and biofilm formation in vibrio parahaemolyticus]. | to study the regulation mechanism of biofilm formation c-di-gmp synthesisby apha in vibrio parahaemolyticus, by using phenotypic and molecular biochemical experiments. | 2014 | 25199251 |
| ptlgbp, a pattern recognition receptor in portunus trituberculatus involved in the immune response against different challenges. | lipopolysaccharide and b-1,3-glucan binding protein (lgbp) is a pattern recognition receptor that can recognize and bind lps and b-1,3-glucan. lgbp has crucial roles in innate immune defense against gram-negative bacteria and fungi. in this study, lgbp functions in portunus trituberculatus innate immunity were analyzed. first, the mrna expression of ptlgbp in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (pamps) stimulations were examined using ... | 2014 | 25194733 |
| enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain). | in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ... | 2014 | 25193866 |
| draft genome sequences of four strains of vibrio parahaemolyticus, three of which cause early mortality syndrome/acute hepatopancreatic necrosis disease in shrimp in china and thailand. | we sequenced four vibrio parahaemolyticus strains, three of which caused serious acute hepatopancreatic necrosis disease. sequence analysis of the virulent strains revealed not only genes related to cholera toxin and the type iv pilus/type iv secretion system but also a unique, previously unreported, large extrachromosomal plasmid that encodes a homolog to the insecticidal photorhabdus insect-related binary toxin pirab. | 2014 | 25189578 |
| purification, characterization and production optimization of a vibriocin produced by mangrove associated vibrio parahaemolyticus. | to identify a potential bacterium which produces antimicrobial peptide (vibriocin), and its purification, characterization and production optimization. the bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem. | 2014 | 25182547 |
| prevalence and characterisation of non-cholerae vibrio spp. in final effluents of wastewater treatment facilities in two districts of the eastern cape province of south africa: implications for public health. | vibrios and other enteric pathogens can be found in wastewater effluents of a healthy population. we assessed the prevalence of three non-cholerae vibrios in wastewater effluents of 14 wastewater treatment plants (wwtp) in chris hani and amathole district municipalities in the eastern cape province of south africa for a period of 12 months. with the exception of wwtp10 where presumptive vibrios were not detected in summer and spring, presumptive vibrios were detected in all seasons in other wwtp ... | 2015 | 25167817 |
| identification and expression analysis of immune-related genes linked to rel/nf-κb signaling pathway under stresses and bacterial challenge from the small abalone haliotis diversicolor. | inhibitor of nf-κb (iκb), nuclear factor-κb (nf-κb), and akirin2 are all important members of rel/nf-κb signaling pathway, which plays a pivotal role in regulating the innate immune response of vertebrates and invertebrates. in this study, the iκb (saiκb) and akirin2 (saakirin2) cdnas of small abalone haliotis diversicolor were cloned and characterized. the full length cdna of saiκb and saakirin2 were 1748 bp and 1452 bp respectively, encoding a protein of 401 aa and 187 aa respectively. a conse ... | 2014 | 25158315 |
| novel myeloid differentiation factor 88, esmyd88, exhibits estube-binding activity in chinese mitten crab eriocheir sinensis. | myeloid differentiation factor 88 (myd88) is a universal and essential adapter protein that participates in the activation of the toll-like receptor/interleukin-1 receptor-mediated signaling pathway. in the present study, a new myd88 gene (named esmyd88) was identified in the chinese mitten crab eriocheir sinensis. the cdna of esmyd88 was 2210 bp long with a 1416 bp open reading frame that encoded a protein with 472 amino acids. predicted esmyd88 protein had a death domain at the n-terminal and ... | 2014 | 25150191 |
| regulation of the expression of the vibrio parahaemolyticus peua gene encoding an alternative ferric enterobactin receptor. | a pvsb-vcta-irga triple deletion mutant of vibrio parahaemolyticus can utilize enterobactin under iron-limiting conditions by inducing a previously undescribed receptor, peua (vpa0150), in response to extracellular alkaline ph and enterobactin. in silico analyses revealed the existence of a two-component regulatory system operon, peurs, immediately upstream of peua, which constitutes an operon with the tonb2 system genes. both the peurs and peua-tonb2 operons were found to be upregulated under i ... | 2014 | 25148374 |
| survival of normal and chlorine-stressed pathogenic and non-pathogenic vibrio parahaemolyticus under adverse conditions. | vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood. the pathogenesis of v. parahaemolyticus is based on the presence of virulence factors: the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh), encoded by the tdh and trh genes, respectively. | 2014 | 25147689 |
| antibacterial effect of silver nanoparticles against four foodborne pathogens. | there is increased demand for improved disinfection methods due to microorganisms resistant to multiple antimicrobial agents. numerous types of disinfectants are available with different properties; but the proper disinfectant must be carefully selected for any specific application to obtain the desired antimicrobial effect. | 2014 | 25147658 |
| detection of acute hepatopancreatic necrosis disease (ahpnd) in mexico. | acute hepatopancreatic necrosis disease (ahpnd), which has also been referred to as early mortality syndrome (ems), initially emerged as a destructive disease of cultured shrimp species in asia in 2009. the pathogen associated with the disease, vibrio parahaemolyticus, subsequently spread to the western hemisphere and emerged in mexico in early 2013. the spread to the western hemisphere is a major concern to shrimp producers in the region. to date, the only peer-reviewed published method for det ... | 2014 | 25144120 |
| performance comparison of second- and third-generation sequencers using a bacterial genome with two chromosomes. | the availability of diverse second- and third-generation sequencing technologies enables the rapid determination of the sequences of bacterial genomes. however, identifying the sequencing technology most suitable for producing a finished genome with multiple chromosomes remains a challenge. we evaluated the abilities of the following three second-generation sequencers: roche 454 gs junior (gs jr), life technologies ion pgm (ion pgm), and illumina miseq (miseq) and a third-generation sequencer, t ... | 2014 | 25142801 |
| remodeling of the intestinal brush border underlies adhesion and virulence of an enteric pathogen. | intestinal colonization by vibrio parahaemolyticus-the most common cause of seafood-borne bacterial enteritis worldwide-induces extensive disruption of intestinal microvilli. in orogastrically infected infant rabbits, reorganization of the apical brush border membrane includes effacement of some microvilli and marked elongation of others. all diarrhea, inflammation, and intestinal pathology associated with v. parahaemolyticus infection are dependent upon one of its type 3 secretion systems (t3ss ... | 2014 | 25139905 |
| pathogenic vibrios in environmental, seafood and clinical sources in germany. | bacteria of the family vibrionaceae naturally occur in marine and estuarine environments. only few species of vibrionaceae are associated with human cases of gastroenteritis, ear and wound infections, caused by ingestion of seafood or contact with vibrio containing water. increasing consumption of seafood (fish, fishery products and shellfish) poses a possible source of vibrio infections in germany. additionally, there is a growing concern that abundances of pathogenic vibrios may increase in ge ... | 2014 | 25129553 |
| occurrence of the three major vibrio species pathogenic for human in seafood products consumed in france using real-time pcr. | vibrio spp. have emerged as a serious threat to human health worldwide. vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus are of particular concern as they have been linked to gastrointestinal infections and septicemia associated with the consumption of raw or undercooked seafood. we developed hydrolysis probe-based real-time pcr systems with an internal amplification control for the detection of these species. we applied these systems to a total of 167 fresh or frozen crustacean, f ... | 2014 | 25128747 |
| high-quality draft genomes of two vibrio parahaemolyticus strains aid in understanding acute hepatopancreatic necrosis disease of cultured shrimps in mexico. | the high-quality draft genomes of two vibrio parahaemolyticus strains, one that causes the acute hepatopancreatic necrosis disease (ahpnd) in cultured shrimps (fim-s1708(+)), and another that does not (fim-s1392(-)) are reported. a chromosome-scale assembly for the fim-s1392(-) genome is reported here. the analysis of the two genomes gives some clues regarding the genomic differences between the strains. | 2014 | 25125645 |
| phylogenetic diversity and antibacterial activity of culturable fungi derived from the zoanthid palythoa haddoni in the south china sea. | investigation on diversity of culturable fungi mainly focused on sponges and corals, yet little attention had been paid to the fungal communities associated with zoanthid corals. in this study, a total of 193 culturable fungal strains were isolated from the zoanthid palythoa haddoni collected in the south china sea, of which 49 independent isolates were identified using both morphological characteristics and internal transcribed spacer (its) sequence analyses. thirty-five strains were selected f ... | 2015 | 25117478 |
| complete genome sequence of virulent bacteriophage shou24, which infects foodborne pathogenic vibrio parahaemolyticus. | a novel lytic vibrio parahaemolyticus phage (shou24) belonging to the family siphoviridae was isolated from aquatic market sewage. the phage is only able to infect v. parahaemolyticus containing a tdh gene. shou24 has a linear genome of 77,837 bp with a g+c content of 46.0 %. in total, 88 predicted proteins have homologues in databases, and the majority of the core genes share high sequence similarity with genes from unrelated viruses and bacteria. genes related to lysogeny and host lysis were n ... | 2014 | 25115946 |
| phylogenetic and in silico functional analyses of thermostable-direct hemolysin and tdh-related encoding genes in vibrio parahaemolyticus and other gram-negative bacteria. | emergence and spread of pandemic strains of vibrio parahaemolyticus have drawn attention to make detailed study on their genomes. the pathogenicity of v. parahaemolyticus has been associated with thermostable-direct hemolysin (tdh) and/or tdh-related hemolysin (trh). the present study evaluated characteristics of tdh and trh genes, considering the phylogenetic and in silico functional features of v. parahaemolyticus and other bacteria. fifty-two tdh and trh genes submitted to the genbank were an ... | 2014 | 25114910 |
| snapshot of vibrio parahaemolyticus densities in open and closed shellfish beds in coastal south carolina and mississippi. | vibrio parahaemolyticus is a gram negative, halophilic bacterium that is ubiquitous in warm, tropical waters throughout the world. it is a major cause of seafood-associated gastroenteritis and is generally associated with consumption of raw or undercooked seafood, especially oysters. this study presents a snapshot of total v. parahaemolyticus densities in surface waters and shellstock american oysters (crassostrea virginica) from open and closed shellfish harvesting areas, as well as "more rural ... | 2014 | 25106119 |
| draft genome sequences of clinical vibrio parahaemolyticus strains isolated in maryland (2010 to 2013). | vibrio parahaemolyticus is the leading cause of food-borne illnesses associated with the consumption of raw shellfish worldwide. here, we report 45 draft genomes of v. parahaemolyticus. thirty-five of them are strains that were isolated from clinical cases in the state of maryland from 2010 to 2013. the remaining 10 strains were historical isolates, isolated mostly from the west coast of the united states during the period of 1988 to 2004. the availability of these genomes will allow for future ... | 2014 | 25103764 |
| probiotic supplementation influences the diversity of the intestinal microbiota during early stages of farmed senegalese sole (solea senegalensis, kaup 1858). | ingestion of bacteria at early stages results in establishment of a primary intestinal microbiota which likely undergoes several stages along fish life. the role of this intestinal microbiota regulating body functions is crucial for larval development. probiotics have been proved to modulate this microbiota and exert antagonistic effects against fish pathogens. in the present study, we aimed to determine bacterial diversity along different developmental stages of farmed senegalese sole (solea se ... | 2014 | 25103323 |
| vibrio type iii effector vpa1380 is related to the cysteine protease domain of large bacterial toxins. | vibrio parahaemolyticus is a gram-negative halophilic bacterium and one of the leading causes of food-borne gastroenteritis. its genome harbors two type iii secretion systems (t3ss1 and t3ss2), but only t3ss2 is required for enterotoxicity seen in animal models. effector proteins secreted from t3ss2 have been previously shown to promote colonization of the intestinal epithelium, invasion of host cells, and destruction of the epithelial monolayer. in this study, we identify vpa1380, a t3ss2 effec ... | 2014 | 25099122 |
| eating oysters without risk of vibriosis: application of a bacteriophage against vibrio parahaemolyticus in oysters. | vibrio parahaemolyticus is a major cause of foodborne illness and related with the consumption of raw contaminated seafood, especially oysters. to evaluate the effectiveness of various applications of a bacteriophage (phage), pvp-1, against a multiple-antibiotic-resistant v. parahaemolyticus pandemic strain (crs 09-17), we designed artificial contamination models that are most likely to be encountered during oyster processing. when live oysters were treated with bath immersion with pvp-1 after c ... | 2014 | 25086350 |
| impact of seafood regulations for vibrio parahaemolyticus infection and verification by analyses of seafood contamination and infection. | consumption of seafood contaminated with vibrio parahaemolyticus causes foodborne infections, which are on the rise owing to increased consumption of raw seafood in asia, europe, north america, and other regions. v. parahaemolyticus infections have been common in japan since the 1960s. following an epidemic in 1997, the japanese ministry of health, labour, and welfare instituted regulations for seafood in 1999, which appear to be reducing v. parahaemolyticus infections. in this review, we descri ... | 2014 | 25078437 |
| occurrence of potentially pathogenic vibrio in oysters (crassostrea gigas) and waters from bivalve mollusk cultivations in the south bay of santa catarina. | this research aimed to identify and quantify potentially pathogenic vibrio from different cultivations of bivalve shellfish in the state of santa catarina, brazil, and water regions in the south bay, as well as correlate the incidence of these microorganisms with the physicochemical parameters of marine waters. | 2014 | 25075484 |
| copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host ampylation on self-assembled human protein microarrays. | ampylation (adenylylation) is a recently discovered mechanism employed by infectious bacteria to regulate host cell signaling. however, despite significant effort, only a few host targets have been identified, limiting our understanding of how these pathogens exploit this mechanism to control host cells. accordingly, we developed a novel nonradioactive ampylation screening platform using high-density cell-free protein microarrays displaying human proteins produced by human translational machiner ... | 2014 | 25073739 |
| non-immune-related genes and signalling pathways in spleen of vibrio parahaemolyticus-infected epinephelus fuscoguttatus (forskal). | 2015 | 25073481 | |
| prevalence of vibrio parahaemolyticus, and vibrio vulnificus in blue crabs (callinectes sapidus), seawater and sediments of the maryland coastal bays. | to determine the prevalence of total and pathogenic vibrio parahaemolyticus (vp) and v. vulnificus (vv) in blue crabs, water and sediment from the maryland coastal bays (mcbs), usa. | 2014 | 25066367 |
| quorum-quenching activity of the ahl-lactonase from bacillus licheniformis dahb1 inhibits vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality. | vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. vibrio virulence is associated with biofilm formation and is regulated by n-acylated homoserine lactone (ahl)-mediated quorum sensing. in an attempt to reduce vibrio colonisation of shrimps and mortality, we screened native intestinal bacilli from indian white shrimps (fenneropenaeus indicus) for an isolate which s ... | 2014 | 25060960 |