Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| evaluation of ice slurries as a control for postharvest growth of vibrio spp. in oysters and potential for filth contamination. | raw oyster consumption is the most common route of exposure for vibrio spp. infections in humans. vibriosis has been increasing steadily in the united states despite efforts to reduce the incidence of the disease. research has demonstrated that ice is effective in reducing postharvest vibrio spp. growth in oysters but has raised concerns of possible contamination of oyster meat by filth (as indicated by the presence of fecal coliform bacteria or clostridium perfringens). this study examined the ... | 2015 | 26197291 |
| differential expression of catalases in vibrio parahaemolyticus under various stress conditions. | among antioxidant enzymes, catalases protect microorganisms by degrading hydrogen peroxide under oxidative stress. in this study, the activities of at least four vibrio parahaemolyticus catalases (kat1 to kat4) were differentially detected during different growth stages and under various stress conditions using zymographic analysis. our results showed that only kat2 is stable at 55 °c. kat1 and kat2 respond to hydrogen peroxide during the early stationary and exponential growth phases, respectiv ... | 2015 | 26192211 |
| molecular insights into a molluscan transferrin homolog identified from disk abalone (haliotis discus discus) evidencing its detectable role in host antibacterial defense. | the basic function of transferrin is to bind iron (iii) ions in the medium and to deliver them to the locations where they are required for metabolic processes. it also takes part in the host immune defense mainly via its ability to bind to iron (iii) ions. hence, transferrin is also identified as an important acute-phase protein in host immunity. abalones are major shellfish aquaculture crops that are susceptible to a range of marine microbial infections. since transferrin is known to be a majo ... | 2015 | 26191782 |
| a highly sensitive and flexible magnetic nanoprobe labeled immunochromatographic assay platform for pathogen vibrio parahaemolyticus. | a magnetic nanoprobe labeled immunochromatographic test strip (mnp/icts) was developed to detect food-borne pathogen vibrio parahaemolyticus. specific antibody against v. parahaemolyticus was used as test line by coating onto the nitrocellulose membrane. magnetic nanoprobe was prepared by immobilizing the specific antibody onto the surface of superparamagnetic nanoparticles. specificity and sensitivity of the mnp/icts system were verified by artificially contaminated shrimp homogenate samples. r ... | 2015 | 26188497 |
| an improved multiplex real-time sybr green pcr assay for analysis of 24 target genes from 16 bacterial species in fecal dna samples from patients with foodborne illnesses. | here, we developed a new version of our original screening system (rapid foodborne bacterial screening 24; rfbs24), which can simultaneously detect 24 genes of foodborne pathogens in fecal dna samples. this new version (rfbs24 ver. 5) detected all known stx2 subtypes, enterotoxigenic escherichia coli (sth genotype), and vibrio parahaemolyticus (trh2), which were not detected by the original rfbs24 assay. the detection limits of rfbs24 ver. 5 were approximately 5.6 × 10(-2)-5.6 × 10(-5) (ng dna)/ ... | 2016 | 26166499 |
| comprehensive analysis of a vibrio parahaemolyticus strain extracellular serine protease vpsp37. | proteases play an important role in the field of tissue dissociation combined with regenerative medicine. during the years new sources of proteolytic enzymes have been studied including proteases from different marine organisms both eukaryotic and prokaryotic. herein we have purified a secreted component of an isolate of vibrio parahaemolyticus, with electrophoretic mobilities corresponding to 36 kda, belonging to the serine proteases family. sequencing of the n-terminus enabled the in silico id ... | 2015 | 26162075 |
| vibrio parahaemolyticus: a rare cause of chronic diarrhea in a heart transplant patient. | vibrio parahaemolyticus usually causes a self-limiting acute diarrheal illness, and is rarely tested for in cases of chronic diarrhea. we present a rare case of chronic diarrhea caused by v. parahaemolyticus in a heart transplant patient requiring antibiotic treatment. | 2014 | 26157876 |
| dual function of a bacterial protein as an adhesin and extracellular effector of host gtpase signaling. | bacterial pathogens often target conserved cellular mechanisms within their hosts to rewire signaling pathways and facilitate infection. rho gtpases are important nodes within eukaryotic signaling networks and thus constitute a common target of pathogen-mediated manipulation. a diverse array of microbial mechanisms exists to interfere with rho gtpase signaling. while targeting of gtpases by secreted bacterial effectors is a well-known strategy bacterial pathogens employ to interfere with the hos ... | 2015 | 26156628 |
| dna-binding protein hu coordinates pathogenicity in vibrio parahaemolyticus. | hu is one of the most abundant nucleoid-associated proteins in bacterial cells and regulates the expression of many genes involved in growth, motility, metabolism, and virulence. it is known that vibrio parahaemolyticus pathogenicity is related to its characteristic rapid growth and that type iii secretion system 1 (t3ss1) contributes to its cytotoxicity. however, it is not known if hu plays a role in the pathogenicity of v. parahaemolyticus. in the present study, we investigated the effect of h ... | 2015 | 26148713 |
| the structure of a gfp-based antibody (fluorobody) to tlh, a toxin from vibrio parahaemolyticus. | a fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (gfp) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a gfp. it is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. here, the expression, purification and crystal structure of a recombinant fluorobody for tlh (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteri ... | 2015 | 26144238 |
| oxidative stress response of the black tiger shrimp penaeus monodon to vibrio parahaemolyticus challenge. | vibrio parahaemolyticus is a virulent pathogen that affects shrimp aquaculture. reactive oxygen species are produced by the immune system that defends the host against foreign microorganisms. in the present study, the oxidative stress response in hepatopancreas and gills of penaeus monodon to v. parahaemolyticus challenge were studied, such as respiratory burst, ros production (·o2(-) and ·oh), activities of antioxidant enzymes (cat, gpx, sod, pod and gst) and oxidative damage to lipid and prote ... | 2015 | 26142143 |
| draft genome sequence of vibrio parahaemolyticus vh3, isolated from an aquaculture environment in greece. | vibrio parahaemolyticus is an important foodborne pathogen responsible for gastroenteritis outbreaks globally. it has also been identified as an important pathogen in aquatic organisms. here, we report a draft genome sequence of v. parahaemolyticus, strain vh3, isolated from farmed juvenile greater amberjack, seriola dumerili, in greece. | 2015 | 26139725 |
| [analysis of antimicrobial resistance results of vibrio parahaemolyticus isolated from shellfish and its habitat in 2013]. | to evaluate the antimicrobial resistance of vibrio parahaemolyticus isolated from shellfish and its habitat in sichuan, fujian and guangxi. | 2015 | 26137614 |
| gene expression profiles of vibrio parahaemolyticus in the early stationary phase. | vibrio (v.) parahaemolyticus is an aquatic bacterium capable of causing foodborne gastroenteritis. in the environment or the food chain, v. parahaemolyticus cells are usually forced into the stationary phase, the common phase for bacterial survival in the environment. so far, little is known about whole genomic expression of v. parahaemolyticus in the early stationary phase compared with the exponential growth phase. we performed whole transcriptomic profiling of v. parahaemolyticus cells in bot ... | 2015 | 26118852 |
| cloning and characterization of a novel hemocyanin variant lvhmcv4 from shrimp litopenaeus vannamei. | recently, we found 3 variants of hemocyanin subunit with higher molecular weight in shrimp litopenaeus vannamei (named as lvhmcv1-3). in this study, a novel l. vannamei hemocyanin variant (named as lvhmcv4) was further cloned and characterized. bioinformatic analysis predicted that lvhmcv4 contains one open reading frame of 2137 bp and encodes a polypeptide of 678 amino acids. it shares 84-99% cdna sequences identity to that of the classical form of l. vannamei hemocyanin (lvhmc, aj250830.1) and ... | 2015 | 26115633 |
| examination of clinical and environmental vibrio parahaemolyticus isolates by multi-locus sequence typing (mlst) and multiple-locus variable-number tandem-repeat analysis (mlva). | vibrio parahaemolyticus is a leading cause of seafood-borne infections in the us. this organism has a high genetic diversity that complicates identification of strain relatedness and epidemiological investigations. however, sequence-based analysis methods are promising tools for these identifications. in this study, multi-locus sequence typing (mlst) and multiple-locus variable-number tandem-repeat analysis (mlva) was performed on 58 v. parahaemolyticus isolates (28 of oyster and 30 of clinical ... | 2015 | 26113844 |
| identification and purification of the cpd photolyase in vibrio parahaemolyticus rimd2210633. | photoreactivation is an error-free mechanism of dna repair, utilized by prokaryotes and most eukaryotes and is catalyzed by specific enzymes called dna photolyases. photoreactivation has been reported in vibrio parahaemolyticus wp28; however, information on photolyases in v. parahaemolyticus (v.p) strains has not been reported. this study examined the photoreactivation in v.p rimd2210633. the photolyase responsible for repairing cyclobutane pyrimidine dimer (cpd) in dna was identified, and the c ... | 2015 | 26104028 |
| identification and functional characterization of the tab2 gene from litopenaeus vannamei. | in drosophila, tab2, an important intermediate in the imd signaling pathway, plays critical roles in the innate immune response in response to bacterial and viral infection. however, the role of tab-related proteins in the immune response of shrimp has not yet been established. here, we reported the identification of a tab2-like gene in litopenaeus vannamei designated as lvtab2. the full-length cdna of lvtab2 was 2160 bp with an open reading frame of 1827 bp, which encoded a putative protein of ... | 2015 | 26102459 |
| development of a rapid and simple immunochromatographic assay to identify vibrio parahaemolyticus. | to rapidly and simply determine whether or not bacterial colonies growing on agar were vibrio parahaemolyticus, we developed an immunochromatographic assay (vp-ica) using two different monoclonal antibodies (designated mab-vp34 and mab-vp109) against the delta subunit of v. parahaemolyticus-f0f1 atp synthase. the epitopes recognized by mab-vp34 and mab-vp109 were mapped to sequences of eight ((47)lltssfsa(54)) and six amino acid residues ((16)fdfavd(21)), respectively. an amino acid sequence sim ... | 2015 | 26093260 |
| prevalence of vibrio spp. in raw shrimps (parapenaeus longirostris) and performance of a chromogenic medium for the isolation of vibrio strains. | vibrios are natural inhabitants of estuarine ecosystems and some species may pose public health problem as agents of sporadic or collective food-borne infections associated with the consumption of fish or shellfish. samples of raw shrimp (n = 299), fished in coastal areas of the city of agadir, morocco, and collected from its fish marketplace, were examined for the presence of pathogenic vibrios. microbiological analysis was carried out according to a protocol using thiosulphate citrate bile suc ... | 2015 | 26081523 |
| temporal and spatial variation in the abundance of total and pathogenic vibrio parahaemolyticus in shellfish in china. | we investigated the abundance of total and pathogenic vibrio parahaemolyticus in shellfish sampled from four provinces in china during may 2013 and march 2014 using the most probable number-polymerase chain reaction (mpn-pcr) method. total v. parahaemolyticus was detected in 67.7% of 496 samples. a total of 38.1% and 10.1% of samples exceeded 1,000 mpn g(-1) and 10,000 mpn g(-1), respectively. v. parahaemolyticus densities followed a seasonal and geographical trend, with guangxi and sichuan shel ... | 2015 | 26061712 |
| prevalence, biochemical characteristics, and antibiotic susceptibility of aeromonads, vibrios, and plesiomonads isolated from different sources at a zoo. | aeromonas spp., vibrio parahaemolyticus , and plesiomonas shigelloides are commonly implicated in foodborne and waterborne diarrheal illnesses of humans and other animals. the present study assessed the prevalence, biochemical characteristics, and antibiotic susceptibility of aeromonas spp., v. parahaemolyticus , and p. shigelloides by analyzing samples from 729 sources at a zoo, including animal feces (n=607), watering facilities (n=104), and pond water samples (n=18). of the 729 samples collec ... | 2015 | 26056883 |
| corrigendum: the pathogenesis, detection, and prevention of vibrio parahaemolyticus. | [this corrects the article on p. 144 in vol. 6, pmid: 25798132.]. | 2015 | 26052313 |
| quantifying viable vibrio parahaemolyticus and listeria monocytogenes simultaneously in raw shrimp. | a novel taqman-based multiplex real-time pcr method combined with propidium monoazide (pma) treatment was firstly developed for the simultaneous quantification of viable vibrio parahaemolyticus and listeria monocytogenes in raw shrimp. the optimization of pma concentration showed that 100 μm was considered optimal to effectively inhibit 10(8) cfu/ml dead cells of both bacteria. the high specificity of this method was confirmed on tests using 96 target and non-target strains. the optimized assay ... | 2015 | 26048473 |
| a repeat unit of vibrio diarrheal t3s effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments. | a novel bacterial type iii secretion effector, vopv, from the enteric pathogen vibrio parahaemolyticus has been identified as a key factor in pathogenicity due to its interaction with cytoskeletal actin. one of the repeat units in the long repetitive region of vopv, named vopv(rep1), functions as an actin-binding module. despite its importance in pathogenesis, the manner in which the effector binds to actin and the subsequent effects on actin dynamics remain unclear. here, we report the molecula ... | 2015 | 26039684 |
| suspension of oysters reduces the populations of vibrio parahaemolyticus and vibrio vulnificus. | vibrio parahaemolyticus (vp) and vibrio vulnificus (vv) are associated with the consumption of raw oysters and cause illnesses ranging from simple gastroenteritis to life-threatening septicaemia. these halophilic bacteria are frequently found in marine and estuarine systems, accumulating within the tissues of a number of aquatic organisms and passing on to humans after consumption, through contaminated water, or via open wounds. as benthic organisms capable of filtering 40 gallons of water per h ... | 2015 | 26031606 |
| identification, characterization, and functional studies of a pelle gene in the chinese mitten crab, eriocheir sinensis. | the toll-like receptor/nf-κb signaling pathways play an important role in the innate immune system. in the present study, one pelle gene (named espelle) was identified for the first time from the chinese mitten crab eriocheir sinensis. the full-length cdna of espelle is 3797 bp with a 3156 bp-long open reading frame that encodes a 1051 amino acid protein. espelle protein contains a death domain at the n-terminal and a serine/threonine kinase domain at the c-terminal. a neighbor joining phylogene ... | 2015 | 26026692 |
| two variants of selenium-dependent glutathione peroxidase from the disk abalone haliotis discus discus: molecular characterization and immune responses to bacterial and viral stresses. | glutathione peroxidase (gpx) is an essential member of the antioxidant systems of living organisms and may be involved in immune defense against pathogenic invasion. in the current study, two selenium-dependent glutathione peroxidases (absegpxs) that shared 54.3% identity were identified from the disk abalone haliotis discus discus. the open reading frames (orfs) of absegpx-a and absegpx-b coded for 222 and 220 amino acids, respectively, with a characteristic selenocysteine residue encoded by an ... | 2015 | 26025184 |
| draft genome sequence of strain atcc 17802(t), the type strain of vibrio parahaemolyticus. | we report the draft genome of vibrio parahaemolyticus atcc 17802(t), containing 5067729 bp. the g+c content of the genome is 45.24 %. this strain possesses genes encoding a type iii secretion system 1, a type iii secretion system 2 and a tdh related hemolysin (trh). its taxonomically important phenotypes were also experimentally characterized. | 2015 | 26021549 |
| characterization and pcr detection of binary, pir-like toxins from vibrio parahaemolyticus isolates that cause acute hepatopancreatic necrosis disease (ahpnd) in shrimp. | unique isolates of vibrio parahaemolyticus (vpahpnd) have previously been identified as the causative agent of acute hepatopancreatic necrosis disease (ahpnd) in shrimp. ahpnd is characterized by massive sloughing of tubule epithelial cells of the hepatopancreas (hp), proposed to be induced by soluble toxins released from vpahpnd that colonize the shrimp stomach. since these toxins (produced in broth culture) have been reported to cause ahpnd pathology in reverse gavage bioassays with shrimp, we ... | 2015 | 26017673 |
| [comparative study of survival vibrio parahaemolyticus with escherichia coli and salmonella typhimurium in seawater]. | in this work, survival tests are conducted in oligotrophic seawater using pathogenic bacterial strains: escherichia coli entéroagrégative, salmonella typhimurium and vibrio parahaemolyticus. after 26 days of incubation in seawater, the three bacterial strains are exposed to sunlight for nine hours. bacterial cells of the three strains, recovered at the end of the experiment by centrifugation were tested for their sensitivity to antibiotics and their enzymatic and metabolic profile (api 20e and 2 ... | 2013 | 26012210 |
| molecular characterization of a transmembrane c-type lectin receptor gene from ayu (plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages. | c-type lectin receptors (ctlrs) play vital roles in immune responses as pattern-recognition receptors (prrs). in this study, we identified a novel c-type lectin receptor (pactlrc) gene from ayu, plecoglossus altivelis. predicted pactlrc is a single transmembrane receptor with a typical carbohydrate recognition domain (crd) at its c-terminus. sequence comparison and phylogenetic tree analysis showed that pactlrc was most closely related to atlantic salmon (salmo salar) clrc, but was significantly ... | 2015 | 26010409 |
| a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis). | integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ... | 2015 | 26004499 |
| inactivation of vibrio parahaemolyticus by antimicrobial photodynamic technology using methylene blue. | vibrio parahaemolyticus is the leading causative pathogen of gastroenteritis often related to contaminated seafood. photodynamic inactivation has been recently proposed as a strategy for killing cells and viruses. the objective of this study was to verify the bactericidal effects caused by photodynamic inactivation using methylene blue (mb) over v. parahaemolyticus via flow cytometry, agarose gel electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis. vibrio parahaemolytic ... | 2016 | 25989459 |
| detection of acute hepatopancreatic necrosis disease strain of vibrio parahaemolyticus using loop-mediated isothermal amplification. | 2016 | 25988949 | |
| the first report of vibrio parahaemolyticus strain o10:k60 in japan, a new combination of o and k serotypes isolated from a patient with gastroenteritis. | vibrio parahaemolyticus is an important pathogen that causes gastroenteritis in humans, generally associated with the consumption of contaminated seafood, particularly raw shellfish. there are many serotypes in v. parahaemolyticus resulting from a combination of o and k antigens. among them, o3:k6 and their variants, which represent the pandemic clone, are the most widespread strains worldwide. in this study, we examined v. parahaemolyticus isolated from a gastroenteritis patient's stool at a ho ... | 2016 | 25971326 |
| deep-sea hydrothermal vent bacteria related to human pathogenic vibrio species. | vibrio species are both ubiquitous and abundant in marine coastal waters, estuaries, ocean sediment, and aquaculture settings worldwide. we report here the isolation, characterization, and genome sequence of a novel vibrio species, vibrio antiquarius, isolated from a mesophilic bacterial community associated with hydrothermal vents located along the east pacific rise, near the southwest coast of mexico. genomic and phenotypic analysis revealed v. antiquarius is closely related to pathogenic vibr ... | 2015 | 25964331 |
| [molecular genetic makers for vibrio parahaemolyticus--a review]. | vibrio parahaemolyticus is an important foodborne pathogen, of which the 03:k6 serotype caused many outbreaks in different countries since 1996. based on the 10 years data (1992-2001) from china, gastroenteritis caused by vibrio parahaemolyticus accounted for 31.1% of foodborne disease outbreaks that were resulted from microorganisms. most environmental strains of vibrio parahaemolyticus are non-pathogenic strains. however, clinical strains can producethermostable direct hemolysin (tdh), tdh-rel ... | 2015 | 25958678 |
| daily variations in pathogenic bacterial populations in a monsoon influenced tropical environment. | changing climatic conditions have influenced the monsoon pattern in recent years. variations in bacterial population in one such tropical environment were observed everyday over two years and point out intra and inter annual changes driven by the intensity of rainfall. vibrio spp. were abundant during the monsoon and so were faecal coliforms. vibrio alginolyticus were negatively influenced by nitrate, whereas, silicate and rainfall positively influenced vibrio parahaemolyticus numbers. it is als ... | 2015 | 25956443 |
| prevalence and antimicrobial resistance of vibrio parahaemolyticus isolated from raw shellfish in poland. | vibrio parahaemolyticus is a marine bacterium recognized as an important cause of gastroenteritis in humans consuming contaminated shellfish. in recent years, increasing resistance to ampicillin and aminoglycosides has been observed among v. parahaemolyticus isolates. however, the first-line antimicrobials such as tetracyclines and fluoroquinolones remained highly effective against these bacteria. the aim of this study was to evaluate the occurrence of v. parahaemolyticus in live bivalve mollusc ... | 2015 | 25951402 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae, and vibrio vulnificus in the aquacultural environments of taiwan. | the occurrence of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae in a total of 72 samples from six aquaculture ponds for groupers, milk fish, and tilapia in southern taiwan was examined by the membrane filtration and colony hybridization method. the halophilic v. parahaemolyticus was only recovered in seawater ponds, with a high isolation frequency of 86.1% and a mean density of 2.6 log cfu/g. v. cholerae was found in both the seawater and freshwater ponds but preferentially in ... | 2015 | 25951392 |
| rna-seq analysis of macrobrachium rosenbergii hepatopancreas in response to vibrio parahaemolyticus infection. | the malaysian giant freshwater prawn, macrobrachium rosenbergii, is an economically important crustacean worldwide. however, production of this prawn is facing a serious threat from vibriosis disease caused by vibrio species such as vibrio parahaemolyticus. unfortunately, the mechanisms involved in the immune response of this species to bacterial infection are not fully understood. we therefore used a high-throughput deep sequencing technology to investigate the transcriptome and comparative exp ... | 2015 | 25922623 |
| pathogenic vibrio parahaemolyticus isolated from biofouling on commercial vessels and harbor structures. | ballast water is a significant vector of microbial dissemination; however, biofouling on commercial vessel hulls has been poorly studied with regard to pathogenic bacteria transport. biofouling on three commercial vessels and seven port structures in ensenada, baja california, mexico, was examined by qpcr to identify and quantify vibrio parahaemolyticus, a worldwide recognized food-borne human pathogen. pathogenic variants (trh+, tdh+) of v. parahaemolyticus were detected in biofouling homogenat ... | 2015 | 25921866 |
| protein transduction domain of transactivating transcriptional activator fused to outer membrane protein k of vibrio parahaemolyticus to vaccinate marbled eels (anguilla marmorata) confers protection against mortality caused by v. parahaemolyticus. | although immersion and oral vaccination are the most practical methods for fish farmers, their applications are very limited due to low immune stimulation effect. we used the protein transduction domain (ptd) of transactivating transcriptional factor (tat) derived from hiv tat protein to increase the delivery efficiency of aquatic protein vaccines. vibrio parahaemolyticus outer membrane protein k (ompk), a reported vaccine candidate for the prevention of v. parahaemolyticus infection, was fused ... | 2015 | 25919337 |
| first detection of ampc β-lactamase bla(cmy-2) on a conjugative inca/c plasmid in a vibrio parahaemolyticus isolate of food origin. | vibrio parahaemolyticus is an important causative agent of gastroenteritis, with the consumption of contaminated seafood being the major transmission route. resistance to penicillin is common among v. parahaemolyticus strains, whereas cephalosporin resistance remains rare. in an attempt to assess the current prevalence and characteristics of antibiotic resistance of this pathogen in common food samples, a total of 54 (17% of the total samples) v. parahaemolyticus strains were isolated from 318 m ... | 2015 | 25918142 |
| daxx from pacific white shrimp litopenaeus vannamei is involved in activation of nf-κb pathway. | death domain-associated factor 6 (daxx) is a fas-binding protein that mediates the activation of jun amino-terminal kinase (jnk) pathway and fas-induced apoptosis. in this study, a crustacean daxx (lvdaxx) was firstly cloned and identified from pacific white shrimp litopenaeus vannamei. the lvdaxx cdna was 2644 bp in length with an open reading frame (orf) of 2217 bp. sequence analysis indicated that lvdaxx contained a single daxx domain and two nuclear localization signals (nlss) and shared a s ... | 2015 | 25917972 |
| three novel clade b serine protease inhibitors from disk abalone, haliotis discus discus: molecular perspectives and responses to immune challenges and tissue injury. | serine protease inhibitors (serpins) control cellular protease activity in order to maintain cellular homeostasis. the immune and inflammatory responses of invertebrate clade b serpins have not been widely reported. in the present study, three proteins with high similarity to clade b serpins, referred to as abserpin-1, abserpin-2 and abserpin-3, were identified from disk abalone (haliotis discus discus). while abserpin-1 (399 aa) was of a typical size for this protein class, abserpin-2 (506 aa) ... | 2015 | 25917971 |
| prevalence and genetic diversity of clinical vibrio parahaemolyticus isolates from china, revealed by multilocus sequence typing scheme. | the population structure of clinical vibrio parahaemolyticus isolates spreading in china remains undefined. we brought 218 clinical isolates from the pubmlst database originating from different regions of china collected since the year of 1990, analyzed by multilocus sequence typing (mlst), to elucidate the prevalence and genetic diversity of v. parahaemolyticus circulating in chinese population. the mlst scheme produced 137 sequence types (sts). these sts were clustered into six clonal complexe ... | 2015 | 25914691 |
| improvement of the quantitation method for the tdh (+) vibrio parahaemolyticus in molluscan shellfish based on most-probable- number, immunomagnetic separation, and loop-mediated isothermal amplification. | vibrio parahaemolyticus is a marine microorganism that can cause seafood-borne gastroenteritis in humans. the infection can be spread and has become a pandemic through the international trade of contaminated seafood. strains carrying the tdh gene encoding the thermostable direct hemolysin (tdh) and/or the trh gene encoding the tdh-related hemolysin (trh) are considered to be pathogenic with the former gene being the most frequently found in clinical strains. however, their distribution frequency ... | 2015 | 25914681 |
| the c-fos and c-jun from litopenaeus vannamei play opposite roles in vibrio parahaemolyticus and white spot syndrome virus infection. | growing evidence indicates that activator protein-1 (ap-1) plays a major role in stimulating the transcription of immune effector molecules in cellular response to an incredible array of stimuli, including growth factors, cytokines, cellular stresses and bacterial and viral infection. here, we reported the isolation and characterization of a cdna from litopenaeus vannamei encoding the full-length c-fos protein (named as lvc-fos). the predicted amino acid sequences of lvc-fos contained a basic-le ... | 2015 | 25912357 |
| genetic characterization of clinical and environmental vibrio parahaemolyticus from the northeast usa reveals emerging resident and non-indigenous pathogen lineages. | gastric infections caused by the environmentally transmitted pathogen, vibrio parahaemolyticus, have increased over the last two decades, including in many parts of the united states (us). however, until recently, infections linked to shellfish from the cool northeastern us waters were rare. cases have risen in the northeast, consistent with changes in local v. parahaemolyticus populations toward greater abundance or a shift in constituent pathogens. we examined 94 clinical isolates from a perio ... | 2015 | 25904905 |
| correction: characterization of trh2 harbouring vibrio parahaemolyticus strains isolated in germany. | 2015 | 25901603 | |
| opar controls a network of downstream transcription factors in vibrio parahaemolyticus bb22op. | vibrio parahaemolyticus is an emerging world-wide human pathogen that is associated with food-borne gastroenteritis when raw or undercooked seafood is consumed. expression of virulence factors in this organism is modulated by the phenomenon known as quorum sensing, which permits differential gene regulation at low versus high cell density. the master regulator of quorum sensing in v. parahaemolyticus is opar. opar not only controls virulence factor gene expression, but also the colony and cellul ... | 2015 | 25901572 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |
| genome diversification within a clonal population of pandemic vibrio parahaemolyticus seems to depend on the life circumstances of each individual bacteria. | new strains of vibrio parahaemolyticus that cause diarrhea in humans by seafood ingestion periodically emerge through continuous evolution in the ocean. influx and expansion in the southern chilean ocean of a highly clonal v. parahaemolyticus (serotype o3:k6) population from south east asia caused one of the largest seafood-related diarrhea outbreaks in the world. here, genomics analyses of isolates from this rapidly expanding clonal population offered an opportunity to observe the molecular evo ... | 2015 | 25880192 |
| [genetic characterization of vibrio parahaemolyticus o3: k6 serovariant isolated in shenzhen]. | to characterize the o3: k6 serovariant of vibrio parahaemolyticus on virulence gene and molecular typing, and analyze the genetic relationship between o3: k6 and o3: k6 serovariants. | 2015 | 25876490 |
| gene expression profiles of vibrio parahaemolyticus in viable but non-culturable state. | viable but non-culturable (vbnc) state is referred to as a dormant state of non-sporulating bacteria enhancing the survival in adverse environments. to our knowledge, only few studies have been conducted on whole genomic expression of vibrio parahaemolyticus vbnc state. since a degradation of nucleic acids in v. vulnificus non-culturable state has been detected, we hypothesize that gene regulation of vbnc cells is highly reduced, downregulation of gene expression is dominant and only metabolic f ... | 2015 | 25873464 |
| effect of sodium-alginate and laminaran on salmonella typhimurium infection in human enterocyte-like ht-29-luc cells and balb/c mice. | brown algal polysaccharides such as alginate, polymers of uronic acids, and laminaran, beta-1,3 and 1,6-glucan, can be fermented by human intestinal microbiota. to evaluate the effects of these polysaccharides on infections caused by food poisoning pathogens, we investigated the adhesion and invasion of pathogens (salmonella typhimurium, listeria monocytogenes and vibrio parahaemolyticus) in human enterocyte-like ht-29-luc cells and in infections caused in balb/c mice. both sodium na-alginate an ... | 2015 | 25857966 |
| a pandemic vibrio parahaemolyticus o3:k6 clone causing most associated diarrhea cases in the pacific northwest coast of mexico. | between september and october of 2004, more than 1230 cases of gastroenteritis due to pandemic o3:k6 strains of vibrio parahaemolyticus (v. parahaemolyticus) were reported in the relatively small geographical area of southern sinaloa, a state located in northwest mexico. since then, v. parahaemolyticus-associated gastroenteritis cases have gradually increased in prevalence spreading from south to north. the present study conducted an epidemiological surveillance of v. parahaemolyticus strains in ... | 2015 | 25852677 |
| insights into the environmental reservoir of pathogenic vibrio parahaemolyticus using comparative genomics. | vibrio parahaemolyticus is an aquatic halophilic bacterium that occupies estuarine and coastal marine environments, and is a leading cause of seafood-borne food poisoning cases. to investigate the environmental reservoir and potential gene flow that occurs among v. parahaemolyticus isolates, the virulence-associated gene content and genome diversity of a collection of 133 v. parahaemolyticus isolates were analyzed. phylogenetic analysis of housekeeping genes, and pulsed-field gel electrophoresis ... | 2015 | 25852665 |
| biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. | in the present study, the strain brevibacterium frigoritolerans dc2 was explored for the efficient and extracellular synthesis of silver nanoparticles. these biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. in addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. the dynamic l ... | 2015 | 25848272 |
| draft whole-genome sequences of 14 vibrio parahaemolyticus clinical isolates with an ambiguous k serogroup. | vibrio parahaemolyticus is a bacterial pathogen responsible for mild to severe gastroenteritis, wound infections, and septicemia resulting from the ingestion or handling of raw or undercooked contaminated seafood. here, we report the draft whole-genome sequences and annotations of 14 canadian v. parahaemolyticus clinical isolates that were serologically identified as k group ii using polyvalent antisera but were not specifically k serogrouped using monovalent antisera. | 2015 | 25838480 |
| use of whole-genome phylogeny and comparisons for development of a multiplex pcr assay to identify sequence type 36 vibrio parahaemolyticus. | vibrio parahaemolyticus sequence type 36 (st36) strains that are native to the pacific ocean have recently caused multistate outbreaks of gastroenteritis linked to shellfish harvested from the atlantic ocean. whole-genome comparisons of 295 genomes of v. parahaemolyticus, including several traced to northeastern u.s. sources, were used to identify diagnostic loci, one putatively encoding an endonuclease (prp), and two others potentially conferring o-antigenic properties (cps and flp). the combin ... | 2015 | 25832299 |
| dimeric wh2 repeats of vopf sequester actin monomers into non-nucleating linear string conformations: an x-ray scattering study. | vopf and vopl are highly similar virulence-factors of vibrio cholerae and vibrio parahaemolyticus respectively that disrupt the host's actin cytoskeleton, using a unique organization in dimerized wh2 repeats. association of dimerized wh2 domains with the barbed face of actin confers multifunctional activities to vopf in vitro, including g-actin sequestration and filament nucleation, barbed end tracking and uncapping. here, small angle x-ray scattering (saxs) measurements of complexes of vopf wit ... | 2015 | 25818509 |
| [collection of vibrio parahaemolyticus species members: phenotypic and genotypic characteristics]. | formation of vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology. | 2015 | 25816506 |
| complete genome sequences of a clinical isolate and an environmental isolate of vibrio parahaemolyticus. | vibrio parahaemolyticus is the leading cause of seafood-borne infections in the united states. we report complete genome sequences for two v. parahaemolyticus strains isolated in 2007, cdc_k4557 and fda_r31 of clinical and oyster origin, respectively. these two sequences might assist in the investigation of differential virulence of this organism. | 2015 | 25814612 |
| effect of climate change on the concentration and associated risks of vibrio spp. in dutch recreational waters. | currently, the number of reported cases of recreational- water-related vibrio illness in the netherlands is low. however, a notable higher incidence of vibrio infections has been observed in warm summers. in the future, such warm summers are expected to occur more often, resulting in enhanced water temperatures favoring vibrio growth. quantitative information on the increase in concentration of vibrio spp. in recreational water under climate change scenarios is lacking. in this study, data on oc ... | 2015 | 25809307 |
| carb-17 family of β-lactamases mediates intrinsic resistance to penicillins in vibrio parahaemolyticus. | vibrio parahaemolyticus is commonly resistant to ampicillin, yet the mechanisms underlying this phenomenon are not clear. in this study, a novel class a carbenicillin-hydrolyzing β-lactamase (carb) family of β-lactamases, bla(carb-17), was identified and found to be responsible for the intrinsic penicillin resistance in v. parahaemolyticus. importantly, bla(carb-17)-like genes were present in all 293 v. parahaemolyticus genome sequences available in genbank and detectable in all 91 v. parahaemol ... | 2015 | 25801555 |
| characterization of trh2 harbouring vibrio parahaemolyticus strains isolated in germany. | vibrio parahaemolyticus is a recognized human enteropathogen. thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) as well as the type iii secretion system 2 (t3ss2) are considered as major virulence factors. as tdh positive strains are not detected in coastal waters of germany, we focused on the characterization of trh positive strains, which were isolated from mussels, seawater and patients in germany. | 2015 | 25799574 |
| the pathogenesis, detection, and prevention of vibrio parahaemolyticus. | vibrio parahaemolyticus, a gram-negative motile bacterium that inhabits marine and estuarine environments throughout the world, is a major food-borne pathogen that causes life-threatening diseases in humans after the consumption of raw or undercooked seafood. the global occurrence of v. parahaemolyticus accentuates the importance of investigating its virulence factors and their effects on the human host. this review describes the virulence factors of v. parahaemolyticus reported to date, includi ... | 2015 | 25798132 |
| identification of immune response-related genes and signalling pathways in spleen of vibrio parahaemolyticus-infected epinephelus fuscoguttatus (forskal) by next-generation sequencing. | 2016 | 25786532 | |
| vgrg2 of type vi secretion system 2 of vibrio parahaemolyticus induces autophagy in macrophages. | type vi secretion system (t6ss) is a macromolecular transenvelope machine encoded within the genomes of several proteobacteria species. vibrio parahaemolyticus contains two putative t6ss systems, vpt6ss1 and vpt6ss2, both contributing to adherence to caco-2 and/or hela cells. however, it remains unknown if these systems are involved in cellular responses. in order to exclude the effects of other virulence factors known to induce cytotoxicity or autophagy, a triple deletion mutant dttt (with dele ... | 2015 | 25784905 |
| complete nucleotide sequence of a conjugative plasmid carrying bla(per-1). | the nucleotide sequence of a self-transmissible plasmid pvph1 harboring bla(per-1) from vibrio parahaemolyticus was determined. pvph1 was 183,730 bp in size and shared a backbone similar to paqu1 and paqu2, differing mainly in an ∼40-kb multidrug resistance (mdr) region. a complex class 1 integron was identified together with iscr1 and bla(per-1) (iscr1-bla(per-1)-gst-abct-qaceδ1-sul1), which was shown to form a circular intermediate playing an important role in the dissemination of bla(per-1). | 2015 | 25779581 |
| the development of a green approach for the biosynthesis of silver and gold nanoparticles by using panax ginseng root extract, and their biological applications. | the biosynthesis of nanoparticles has received attention because of the development of economic and environmentally friendly technology for the synthesis of nanoparticles. the study develops a convenient method for the green synthesis of silver and gold nanoparticles by utilizing fresh root extract of the four-year old panax ginseng plant, and evaluated the antimicrobial applications of silver nanoparticles against pathogenic microorganisms. p. ginseng is a well-known herbal medicinal plant, and ... | 2016 | 25771716 |
| green synthesis of silver nanoparticles by bacillus methylotrophicus, and their antimicrobial activity. | the synthesis of silver nanoparticles (agnps) by microorganisms is an area attracting growing interest in nanobiotechnology, due to the applications of these nanoparticles in various products including cosmetics and biosensors, and in the biomedical, clinical, and bioimaging fields as well. various microorganisms have been found to be able to synthesize agnps when silver salts are supplied in the reaction system. the main objectives of this study were to evaluate the efficiency of synthesis of a ... | 2016 | 25749281 |
| characterization of vibrio parahaemolyticus clinical strains from maryland (2012-2013) and comparisons to a locally and globally diverse v. parahaemolyticus strains by whole-genome sequence analysis. | vibrio parahaemolyticus is the leading cause of foodborne illnesses in the us associated with the consumption of raw shellfish. previous population studies of v. parahaemolyticus have used multi-locus sequence typing (mlst) or pulsed field gel electrophoresis (pfge). whole genome sequencing (wgs) provides a much higher level of resolution, but has been used to characterize only a few united states (us) clinical isolates. here we report the wgs characterization of 34 genomes of v. parahaemolyticu ... | 2015 | 25745421 |
| hygiene and health risks associated with the consumption of edible lamellibranch molluscs. | edible lamellibranch molluscs are frequently the cause of very serious outbreaks of food poisoning. microbiological suitability for human consumption, according to ec reg. n. 1441/2007, is only based on two faecal bacteriological contamination parameters (escherichia coli and salmonella) and does not consider other microorganisms which are naturally present within the marine environment and are potentially pathogenic such as those belonging to the genus vibrio. in this study, a total of 540 samp ... | 2015 | 25744696 |
| development and evaluation of a loop-mediated isothermal amplification assay combined with enrichment culture for rapid detection of very low numbers of vibrio parahaemolyticus in seafood samples. | the aim of this study was to develop and evaluate a rapid and effective method to detect vibrio parahaemolyticus, a leading pathogen causing seafood-borne gastroenteritis. a newly designed loop-mediated isothermal amplification (lamp) assay including a short enrichment period was optimized. this assay correctly detected all the target strains (n=61) but none of the non-target strains (n=34). very low numbers of v. parahaemolyticus (2 colony forming unit (cfu) per gram of seafood) could be detect ... | 2015 | 25744462 |
| interaction between the type iii effector vopo and gef-h1 activates the rhoa-rock pathway. | vibrio parahaemolyticus is an important pathogen that causes food-borne gastroenteritis in humans. the type iii secretion system encoded on chromosome 2 (t3ss2) plays a critical role in the enterotoxic activity of v. parahaemolyticus. previous studies have demonstrated that t3ss2 induces actin stress fibers in various epithelial cell lines during infection. this stress fiber formation is strongly related to pathogenicity, but the mechanisms that underlie t3ss2-dependent actin stress fiber format ... | 2015 | 25738744 |
| isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture. | acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ... | 2015 | 25737203 |
| molecular characterization of lvav in response to white spot syndrome virus infection in the pacific white shrimp (litopenaeus vannamei). | litopenaeus vannamei is the most important farmed shrimp species globally, but its production is affected by several factors, including infectious disease. white spot syndrome virus (wssv), in particular, causes significant shrimp losses. to understand the shrimp's immune response against wssv, we cloned lvav from l. vannamei and analyzed its expression pattern in different tissues, in addition to its expression following infection. we employed dsrna and recombinant (r)lvav to explore the potent ... | 2015 | 25735872 |
| intracellular survival of virulence and low-virulence strains of vibrio parahaemolyticus in epinephelus awoara macrophages and peripheral leukocytes. | in this study, we examined the virulence factors and pathogenesis of vibrio parahaemolyticus in epinephelus awoara. the chemotactic motility of v. parahaemolyticus for phagocytosis and intracellular survival in fish macrophages was determined using virulence strains and low-virulence strains of v. parahaemolyticus. we found that the intracellular mean number of virulence strains of v. parahaemolyticus ranged from 0-180 min after co-incubation with macrophages and peripheral leukocytes, was relat ... | 2015 | 25730008 |
| saccharification of β-chitin from squid pen by a fermentation method using recombinant chitinase-secreting escherichia coli. | two strains [bl21(de3) and hms174(de3)] of escherichia coli harboring the recombinant chitinase expression plasmid pvp-chi, which contains vibrio parahaemolyticus chitinase gene with an attached signal sequence, were prepared. these e. coli transformants produced a large amount of recombinant chitinase, which hydrolyzes chitin to yield di-n-acetylchitobiose (glcnac)2, under the presence of isopropyl-1-thio-β-d-galactopyranoside (iptg), and secreted the enzyme into their culture fluid with the ai ... | 2015 | 25725799 |
| biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications. | in the present study, we report a green methodology for the synthesis of silver and gold nanoparticles, using the root extract of the herbal medicinal plant korean red ginseng. the silver and gold nanoparticles were synthesized within 1 h and 10 min respectively. the nanoparticles generated were not aggregated, and remained stable for a long time, which suggests the nature of nanoparticles. the phytochemicals and ginsenosides present in the root extract assist in reducing and stabilizing the syn ... | 2016 | 25706249 |
| incidence of multidrug resistant vibrio parahaemolyticus isolated from ponnani, south india. | the prevalence of vibrio parahaemolyticus has been reported from ponnani earlier, however incidence of multidrug resistant strains have been encountered recently in clinical laboratories. the source for such strains and their presence in this major fish landing centre has been investigated. | 2014 | 25705353 |
| prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from retail shrimps in malaysia. | vibrio parahaemolyticus is a marine and estuarine bacterium that has been the leading cause of foodborne outbreaks which leads to a significant threat to human health worldwide. consumption of seafood contaminated with v. parahaemolyticus causes acute gastroenteritis in individuals. the bacterium poses two main virulence factor including the thermostable direct hemolysin (tdh) which is a pore-forming protein that contributes to the invasiveness of the bacterium in humans and tdh-related hemolysi ... | 2015 | 25688239 |
| characterization and expression analysis of toll-like receptor 2 gene in large yellow croaker, larimichthys crocea. | toll-like receptor 2 (tlr2) plays an important role in innate immune responses. here we describe the isolation and characterization of the full-length cdna sequence of toll-like receptor 2 in large yellow croaker larimichthys crocea (lctlr2). the lctlr2 cdna contains a 5'-terminal untranslated region (5'-utr) of 135 bp, an open reading frame (orf) of 2478 bp encoding a polypeptide of 825 amino acid residues and a 3'-utr of 50 bp. subcellular localization analysis suggested that the lctlr2-pegfp ... | 2015 | 25687392 |
| the expression of propionicin plg-1 gene (plg-1) by lactic starters. | propionicin plg-1 is a bacteriocin produced by propionibacterium thoenii p127. such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic escherichia coli, pseudomonas aeruginosa, vibrio parahaemolyticus, yersinia enterocolitica and a strain of corynebacterium sp. in the present study, plg-1 gene expressing propionicin plg-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. the pcr product cont ... | 2015 | 25684078 |
| molecular cloning and functional characterization of a qm protein in large yellow croaker (larimichthys crocea). | since it was proposed to be a tumor suppressor in 1991, qm protein has attracted intensive and wide attention in plants, animals and fungi research fields. up to date, however, the function of qm protein in fish immunity remains unknown. in this investigation, a qm gene (named as lycqm gene) was cloned from large yellow croaker (larimichthys crocea), and lycqm protein was expressed in escherichia coli and purified. the lycqm gene was ubiquitously transcribed in multi-tissues, including spleen, m ... | 2015 | 25680268 |
| transoceanic spreading of pathogenic strains of vibrio parahaemolyticus with distinctive genetic signatures in the reca gene. | vibrio parahaemolyticus is an important human pathogen whose transmission is associated with the consumption of contaminated seafood. consistent multilocus sequence typing for v. parahaemolyticus has shown difficulties in the amplification of the reca gene by pcr associated with a lack of amplification or a larger pcr product than expected. in one strain (090-96, peru, 1996), the produced pcr product was determined to be composed of two reca fragments derived from different vibrio species. to be ... | 2015 | 25679989 |
| homologs of the acinetobacter baumannii acei transporter represent a new family of bacterial multidrug efflux systems. | multidrug efflux systems are a major cause of resistance to antimicrobials in bacteria, including those pathogenic to humans, animals, and plants. these proteins are ubiquitous in these pathogens, and five families of bacterial multidrug efflux systems have been identified to date. by using transcriptomic and biochemical analyses, we recently identified the novel acei (acinetobacter chlorhexidine efflux) protein from acinetobacter baumannii that conferred resistance to the biocide chlorhexidine, ... | 2015 | 25670776 |
| photorhabdus insect-related (pir) toxin-like genes in a plasmid of vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (ahpnd) of shrimp. | the 69 kb plasmid pvpa3-1 was identified in vibrio parahaemolyticus strain 13‑028/a3 that can cause acute hepatopancreatic necrosis disease (ahpnd). this disease is responsible for mass mortalities in farmed penaeid shrimp and is referred to as early mortality syndrome (ems). the plasmid has a gc content of 45.9% with a copy number of 37 per bacterial cell as determined by comparative quantitative pcr analyses. it consists of 92 open reading frames that encode mobilization proteins, replication ... | 2015 | 25667334 |
| phenotypic and phylogenetic analysis of vibrio parahaemolyticus isolates recovered from diarrhea cases in guangdong province, china. | vibrio parahaemolyticus has emerged as a common foodborne pathogen of global concern. in this study, 108 v. parahaemolyticus isolates that recovered from diarrhea cases (n=96) and seafood products (n=12) in guangdong province from 2007 to 2011 were characterized by serotyping, tdh and trh toxin gene detection and multilocus sequence typing (mlst). the dominant serotypes from the cases were o3:k6, o4:k8 and o1: kut (untyped). however, most isolates recovered from seafood products belonged to othe ... | 2015 | 25662708 |
| roles of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) in vibrio parahaemolyticus. | vibrio parahaemolyticus is the leading cause of seafood borne bacterial gastroenteritis in the world, often associated with the consumption of raw or undercooked seafood. however, not all strains of v. parahaemolyticus are pathogenic. the thermostable direct hemolysin (tdh) or tdh-related hemolysin (trh) encoded by tdh and trh genes, respectively, are considered major virulence factors in v. parahaemolyticus. however, about 10% of clinical strains do not contain tdh and/or trh. environmental iso ... | 2014 | 25657643 |
| development of vibrio spp. infection resistance related snp markers using multiplex snapshot genotyping method in the clam meretrix meretrix. | the clam meretrix meretrix is a commercially important mollusc species in the coastal areas of south and southeast asia. in the present study, large-scale snps were genotyped by the multiplex snapshot genotyping method among the stocks of m. meretrix with different vibrio spp. infection resistance profile. firstly, the autosnp software was applied to mine snps from m. meretrix transcriptome, and 323 snp loci (including 120 indels) located on 64 contigs were selected based on uniprot-go associati ... | 2015 | 25655323 |
| exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. | multidrug-resistant bacteria are an increasingly serious threat to human and animal health. however, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant edwardsiella tarda by gc-ms-based metabolomics. exogenous alanine or glucose restores susceptibility of multidrug-resistant e. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resista ... | 2015 | 25651179 |
| epidemiological evidence of lesser role of thermostable direct hemolysin (tdh)-related hemolysin (trh) than tdh on vibrio parahaemolyticus pathogenicity. | vibrio parahaemolyticus carrying the tdh gene, encoding the thermostable direct hemolysin (tdh), or the trh gene, encoding the tdh-related hemolysin (trh), are both considered virulent strains. there are, however, disproportionally fewer reports of infections caused by seafood contaminated with trh-positive strains than by seafood contaminated with tdh-positive strains. bivalves such as clams and oysters are the major seafood varieties associated with the infections. in this study, the prevalenc ... | 2015 | 25646967 |
| draft genome sequences of four vibrio parahaemolyticus isolates from clinical cases in canada. | vibrio parahaemolyticus is a leading cause of bacterial gastroenteritis following ingestion of contaminated seafood. this report presents the draft genome sequences of four clinical strains of v. parahaemolyticus isolated in canada. all four strains lack traditional pathogenic markers and possess uniquely individual characteristics identified using other typing criteria. | 2015 | 25635013 |
| [etiologic characteristics of vibrio parahaemolyticus strains causing outbreaks and sporadic cases in guangdong, 2013]. | to study the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of vibrio parahaemolyticus isolated from outbreaks and sporadic cases in guangdong, 2013. | 2014 | 25623459 |
| long-term study of vibrio parahaemolyticus prevalence and distribution in new zealand shellfish. | the food-borne pathogen vibrio parahaemolyticus has been reported as being present in new zealand (nz) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown nz seafood. our study determined the current incidence of v. parahaemolyticus in nz oysters and greenshell mussels and the prevalence of v. parahaemolyticus tdh and trh strains. pacific (235) and dredge (21) oyster samples and mussel samples (55) were obtained from commercial shellfish-growing a ... | 2015 | 25616790 |