Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters. | periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ... | 2017 | 28235124 |
| a novel anti-lipopolysaccharide factor spalf6 in mud crab scylla paramamosain exhibiting different antimicrobial activity from its single amino acid mutant. | in crustaceans, anti-lipopolysaccharide factors (alfs) are important immune effectors that have sequence diversity and exhibit broad antimicrobial activities. in this study, we characterized a novel alf homolog spalf6 from mud crab scylla paramamosain and its variant spalf6-v, which was generated by mutations of two amino acids (h46 to r and a110 to p) due to the presence of two single nucleotide polymorphisms (snps). spalf6 was an anionic peptide with isoelectric point (pi) 6.79, whereas spalf6 ... | 2017 | 28232132 |
| molecular detection of the three major pathogenic vibrio species from seafood products and sediments in tunisia using real-time pcr. | vibrio spp. have emerged as a serious threat to human health worldwide. v. parahaemolyticus , v. cholerae , and v. vulnificus pose a considerable public health risk in tunisia because they cause sporadic and epidemic foodborne infections associated with the consumption of raw or undercooked contaminated seafood. more recently, toxr-positive v. alginolyticus was also reported to be a potential source of contaminated seafood. a total of 247 samples, including 113 fishes ( labrus viridis , penaeus ... | 2016 | 28221954 |
| hctoll3 was involved in anti-vibrio defense in freshwater pearl mussel, hyriopsis cumingii. | toll-like receptors (tlrs) play an important role in the activation of innate immune response but their functions in bivalves remain largely unknown. in this study, we identified a tlr from the freshwater pearl mussel hyriopsis cumingii (hctoll3) and investigated its functions in immunity. the full-length cdna of hctoll3 is 3852 bp and includes an open reading frame (orf) of 3228 bp that encodes a polypeptide of 1075 amino acids. the predicted hctoll3 protein shares similar structural characteri ... | 2017 | 28214599 |
| kinneretia thg-sqi4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy. | simple, facile, effective approach for the biosynthesis of silver nanoparticles using kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. kinneretia thg-sqi4 has been isolated from soil sample collected from shangqui, china. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (uv-vis), field emission transmission electron microscopy (fe-tem), energy dispersive x-ray spectroscopy (edx), elemental mapp ... | 2017 | 28211298 |
| microbiological quality and prevalence of β-lactam antibiotic resistance genes in oysters ( crassostrea rhizophorae ). | the microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. the objective of this study was to assess the bacterial composition of the edible oyster crassostrea rhizophorae in urban and preserved estuaries. particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blatem, blashv, and blakpc) via quantitative pcr ... | 2017 | 28207310 |
| a simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus. | an earlier electrochemical mechanism of dna detection was adapted and specified for the detection of vibrio parahaemolyticus in real samples. the reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. detection was assessed using a microprocessor to measure current response under controlled potential. the fabricated sensor was able to specifically distinguish complementary, non-comple ... | 2017 | 28205102 |
| an outbreak of shigellosis in a children welfare institute caused by a multiple-antibiotic-resistant strain of shigella flexneri 2a. | from september 1 to october 27, 2015, an outbreak of bacillary dysentery occurred in the shenzhen children welfare institute (scwi). the shigellosis was uncommon in shenzhen and no related outbreak was reported during the last 5 years. an epidemiological investigation was conducted and the children and nursing workers in scwi were surveyed for gastrointestinal symptoms; 28 of children reported having a diarrheal illness. rectal swabs or fecal specimens from 14 case patients and 24 nursing worker ... | 2017 | 28189512 |
| shrimp ahpnd-causing plasmids encoding the pirab toxins as mediated by pirab-tn903 are prevalent in various vibrio species. | acute hepatopancreatic necrosis disease (ahpnd) is a newly emerging shrimp disease caused by pirab toxins encoded by a plasmid found in vibrio parahaemolyticus. the pirab toxins are the homologs of the photorhabdus insect-related (pir) toxins. here, we report the complete sequences of the ahpnd-causing plasmid isolated from v. owensii, as well as those of its 11 siblings (pvh family). in addition, we also included 13 related plasmids (pvh-r family) without the pirab genes isolated from a variety ... | 2017 | 28169338 |
| vibrio phage kvp40 encodes a functional nad+ salvage pathway. | the genome of t4-type vibrio bacteriophage kvp40 has five genes predicted to encode proteins of pyridine nucleotide metabolism, of which two, nadv and natv, would suffice for an nad(+) salvage pathway. nadv is an apparent nicotinamide phosphoribosyltransferase (namprtase) and natv is an apparent bifunctional nicotinamide mononucleotide adenylyltransferase (nmnatase) and nicotinamide-adenine dinucleotide pyrophosphatase (nudix hydrolase). genes encoding the predicted salvage pathway were cloned, ... | 2017 | 28167526 |
| ecological fitness and virulence features of vibrio parahaemolyticus in estuarine environments. | vibrio parahaemolyticus is a commonly encountered and highly successful organism in marine ecosystems. it is a fast-growing, extremely versatile copiotroph that is active over a very broad range of conditions. it frequently occurs suspended in the water column (often attached to particles or zooplankton), and is a proficient colonist of submerged surfaces. this organism is an important pathogen of animals ranging from microcrustaceans to humans and is a causative agent of seafood-associated food ... | 2017 | 28144705 |
| transcriptome analysis reveals comprehensive insights into the early immune response of large yellow croaker (larimichthys crocea) induced by trivalent bacterial vaccine. | vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. in the present study, we used rna-seq technology to perform transcriptome analysis of spleens from large yellow croaker (larimichthys crocea) induced by inactivated trivalent bacterial vaccine (vibrio parahaemolyticus, vibrio alginolyticus and aeromonas hydrophila). a total of 2,789 or 1,511 differentially expre ... | 2017 | 28135311 |
| genetic and serological identification of three vibrio parahaemolyticus strains as candidates for novel provisional o serotypes. | vibrio parahaemolyticus is a gram-negative, halophilic vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. to date, 13 o serotypes and 71 k serotypes of v. parahaemolyticus have been identified. however, untypeable v. parahaemolyticus strains are frequently found during routine detection, indicating that other forms of serotypes exist and suggesting the necessity for extension of the antigen ... | 2017 | 28129570 |
| biochemical basis for activation of virulence genes by bile salts in vibrio parahaemolyticus. | bile salts act as a stressor to bacteria that transit the intestinal tract. enteric pathogens have hijacked bile as an intestinal signal to regulate virulence factors. we recently demonstrated that vibrio parahemolyticus senses bile salts via a heterodimeric receptor formed by the periplasmic domains of inner-membrane proteins vtra and vtrc. crystal structures of the periplasmic complex reveal that vtra and vtrc form a β-barrel that binds bile salts in its hydrophobic interior to activate the vt ... | 2017 | 28129014 |
| debaryomyces hansenii up regulates superoxide dismutase gene expression and enhances the immune response and survival in pacific red snapper (lutjanus peru) leukocytes after vibrio parahaemolyticus infection. | application of yeast is increasing to improve welfare and promotes growth in aquaculture. the halotolerant yeast debaryomyces hansenii is normally a non-pathogenic yeast with probiotic properties and potential source of antioxidant enzymes as superoxide dismutase. here, first, we characterized the sequence features of mnsod and iccu/znsod from pacific red snapper, and second, we evaluated the potential antioxidant immune responses of the marine yeast debaryomyces hansenii strain cbs004 in leukoc ... | 2017 | 28126556 |
| role of non-coding regulatory rna in the virulence of human pathogenic vibrios. | in recent decades, the identification of small non-coding rnas in bacteria has revealed an important regulatory mechanism of gene expression involved in the response to environmental signals and to the control of virulence. in the family vibrionaceae, which includes several human and animal pathogens, small non-coding rnas (srnas) are closely related to important processes including metabolism, quorum sensing, virulence, and fitness. studies conducted in silico and experiments using microarrays ... | 2016 | 28123382 |
| induction of type i interferons in response to bacterial stimuli in large yellow croaker larimichthys crocea. | in addition to the crucial roles in coordinating antiviral immune responses, type i interferons (ifns) also play a role in the host immunity against bacterial pathogens. our previous study identified two type i ifns from large yellow croaker larimichthys croaea(lc), lcifnd and lcifnh, and showed their strong induction by poly(i:c) and antiviral activities. in the present study, both lcifnd and lcifnh were found to be rapidly induced in head kidney and spleen by mixed bacteria of vibrio alginolyt ... | 2017 | 28119146 |
| detection, enumeration, and isolation of vibrio parahaemolyticus and v. vulnificus from seafood: development of a multidisciplinary protocol. | vibrio parahaemolyticus and v. vulnificus are bacterial foodborne pathogens that can cause illnesses in humans after ingestion or exposure to contaminated seafood or coastal waters. a procedure that combines microbiological, biochemical, and molecular methods was designed and optimized for the detection, enumeration, isolation, and characterization of these clinically significant vibrio spp. initially, microbiological culturing is used to resuscitate and isolate presumptive vibrio spp. from chil ... | 2017 | 28118133 |
| biological therapeutics of pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and mcf-7 breast cancer cells. | the overuse of antimicrobics and drugs has led to the development of resistance in a number of pathogens and parasites, which leads to great concerns for human health and the environment. furthermore, breast cancer is the second most common cause of cancer death in women. mcf-7 is a widely used epithelial cancer cell line, derived from breast adenocarcinoma for in vitro breast cancer studies, since the cell line has retained several ideal characteristics particular to the mammary epithelium. in ... | 2017 | 28115262 |
| transcriptome profiles of the clam meretrix petechialis hepatopancreas in response to vibrio infection. | microbial diseases have received much attention due to their enormous destruction of aquaculture, and vibrio parahaemolyticus is one of the main pathogens that cause bacterial disease in the clam meretrix petechialis. to better understand the molecular mechanisms of the immune response to vibrio in m. petechialis, rna-seq was applied to explore global expression changes of hepatopancreas from this clam after vibrio challenge. there were 199,318,966 clean reads obtained by illumina sequencing, wh ... | 2017 | 28110034 |
| purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties. | the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ... | 2017 | 28110033 |
| high salinity relaying to reduce vibrio parahaemolyticus and vibrio vulnificus in chesapeake bay oysters (crassostrea virginica). | cases of vibrio infections in the united states have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (crassostrea virginica). information is needed on how to reduce numbers of vibrio parahaemolyticus and vibrio vulnificus in bi-valve molluscan shellfish (for example, oysters). the purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (rass) ... | 2017 | 28099766 |
| genetic analysis of vibrio parahaemolyticus o3:k6 strains that have been isolated in mexico since 1998. | vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. environmental and clinical toxigenic strains of v. parahaemolyticus that were isolated in mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the o3:k6 pandemic clone, and their genetic relationship to strains that are r ... | 2017 | 28099500 |
| establishment and validation of rna-based predictive models for understanding survival of vibrio parahaemolyticus in oysters stored at low temperatures. | this study developed rna-based predictive models describing the survival of vibrio parahaemolyticus in eastern oysters (crassostrea virginica) during storage at 0, 4, and 10°c. postharvested oysters were inoculated with a cocktail of five v. parahaemolyticus strains and were then stored at 0, 4, and 10°c for 21 or 11 days. a real-time reverse transcription-pcr (rt-pcr) assay targeting expression of the tlh gene was used to evaluate the number of surviving v. parahaemolyticus cells, which was the ... | 2017 | 28087532 |
| identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn exopalaemon carinicauda. | anti-lipopolysaccharide factor is a kind of antimicrobial peptide (amp) with broad-spectrum activities against bacteria, virus, and fungi in crustacean. different isoforms of alfs showed different activities to virus or bacteria. therefore, discovery of more alfs will provide new insights into drug development and disease control. here we reported an alf gene, ecalf1, isolated from the ridgetail prawn exopalaemon carinicauda. its transcripts was mainly detected in hemocytes. silencing of ecalf1 ... | 2017 | 28082184 |
| vibrio parahaemolyticus o4:k8 forms a potential predominant clone in southern china as detected by whole-genome sequence analysis. | vibrio parahaemolyticus has been the most common food-borne pathogen in southern china, especially the o3:k6 pandemic clone and its serovariants. recently, the serotype o4:k8 became more and more prevalent in southern china, which was different from the o3:k6 pandemic clone. thus, the aim of the present work was to elucidate the molecular characteristics of the o4:k8. some o3:k6 pandemic clone and its serovariants isolated in the same period were selected for comparative analysis, which were sti ... | 2017 | 28073082 |
| quorum sensing regulators are required for metabolic fitness in vibrio parahaemolyticus. | quorum sensing (qs) is a process by which bacteria alter gene expression in response to cell density changes. in vibrio species, at low cell density, the sigma 54-dependent response regulator luxo is active and regulates the two qs master regulators apha, which is induced, and opar, which is repressed. at high cell density the opposite occurs: luxo is inactive, and therefore opar is induced while apha is repressed. in vibrio parahaemolyticus, a significant enteric pathogen of humans, the roles o ... | 2017 | 28069817 |
| identification and functional analysis of a tep gene from a crustacean reveals its transcriptional regulation mediated by nf-κb and jnk pathways and its broad protective roles against multiple pathogens. | thioester-containing proteins (teps) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. in the current study, a tep gene homologous to insect teps (itep) from the crustacean litopenaeus vannamei, named lvtep1, is cloned and functionally characterized. the open reading frame (orf) of lvtep1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kda lvtep1, which is most s ... | 2017 | 28069434 |
| multiple cross displacement amplification combined with gold nanoparticle-based lateral flow biosensor for detection of vibrio parahaemolyticus. | vibrio parahaemolyticus (v. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. in the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (mcda-lfb) to detect the toxr gene of v. parahaemolyticus in dna extracts from pure cultures and spiked oyster homogenates. amplification was carried out at a constant temperature (62°c) for only 30 min, and amplification products were directly applied to ... | 2016 | 28066368 |
| designation of pathogenic resistant bacteria in the sparusaurata sea collected in tunisia coastlines: correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics. | vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from wild and farm sea bream (sparus aurata l.) along the tunisian coast from december 2015 to april ... | 2017 | 28062288 |
| first multi-year retrospective study on vibrio parhaemolyticus and vibrio vulnificus prevalence in ruditapes philippinarum harvested in sacca di goro, italy. | the present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of vibrio spp., and the prevalence of v. parahaemolyticus and v. vulnificus in the manila clam r. philippinarum harvested in sacca di goro, emilia-romagna region, northern italy. on the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have ... | 2016 | 28058248 |
| [monitoring and research on pathogen spectrum in patients with acute diarrhea from sentinel hospital of zhejiang province during 2009 to 2014]. | objective: to explore pathogen spectrum constitution of acute diarrhea in outpatient and emergency of zhejiang province, and provide basis for treatment, prevention and control of the disease. methods: during january 2009 to december 2014, we selected seven sentinel hospitals in different regions of zhejiang, monitored and researched on pathogen spectrum in patients with acute diarrhea from outpatient and emergency. we recorded patients' personal basic information, the main symptoms and signs, a ... | 2016 | 28057113 |
| an integrated rotary microfluidic system with dna extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics. | point-of-care (poc) molecular diagnostics plays a pivotal role for the prevention and treatment of infectious diseases. in spite of recent advancement in microfluidic based poc devices, there are still rooms for development to realize rapid, automatic and cost-effective sample-to-result genetic analysis. in this study, we propose an integrated rotary microfluidic system that is capable of performing glass microbead based dna extraction, loop mediated isothermal amplification (lamp), and colorime ... | 2017 | 28043075 |
| alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp litopenaeus vannamai. | the shrimp multifunctional protein alpha-2-macroglobulin (a2m) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. herein, the function of lva2m from litopenaeus vannamei on the prophenoloxidase (propo) system is reported. the recombinant (r)lva2m produced strongly and specifically inhibited trypsin and the po activity in shrimp plasma in a dose-dependent manner. sile ... | 2017 | 28027986 |
| inactivation of vibrio parahaemolyticus unattached and attached to a solid surface in ph-controlled sodium hypochlorite solutions. | the inactivation of vibrio parahaemolyticus cells that were unattached or attached to a polyethylene terephthalate (pet) disc in ph-controlled sodium hypochlorite (naocl) solutions was studied under turbulent conditions. no significant desorption of attached cells occurred at the free available chlorine (fac) concentrations from 0.1 to 1.0 mg/l. the number of viable cells was estimated by microbial calorimetry. the logarithmic relative reduction of viable cells was proportional to the product of ... | 2016 | 28003634 |
| exse is a negative regulator for t3ss gene expression in vibrio alginolyticus. | type iii secretion systems (t3sss) contribute to microbial pathogenesis of vibrio species, but the regulatory mechanisms are complex. we determined if the classic exsacde protein-protein regulatory model from pseudomonas aeruginosa applies to vibrio alginolyticus. deletion mutants in v. alginolyticus demonstrated that, as expected, the t3ss is positively regulated by exsa and exsc and negatively regulated by exsd and exse. interestingly, deletion of exse enhanced the ability of v. alginolyticus ... | 2016 | 27999769 |
| actin cytoskeleton-modulating t3ss2 effectors and their contribution to the vibrio parahaemolyticus-induced diarrhea. | to understand how bacterial pathogens cause diseases is the most important step in order to prevent the infection and develop an effective treatment. however, the past proceeding studies make us aware of quite-complicated interactions between the host and pathogenic bacteria. vibrio parahaemolyticus, a food-born pathogen that is a subject of our study, causes inflammatory diarrhea in human upon ingestion of contaminated raw or undercooked seafood. many virulence factors has been proposed since i ... | 2016 | 27980291 |
| sequence type 631 vibrio parahaemolyticus, an emerging foodborne pathogen in north america. | 2017 | 27974540 | |
| the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
| molecular cloning and expression analysis of a fish specific interferon regulatory factor, irf11, in orange spotted grouper, epinephelus coioides. | interferon regulatory factors (irfs) are transcription mediators which play vital roles in multiple biological processes, such as antiviral defense, immune response, cell growth regulation and apoptosis. a fish specific irf, termed irf11, has been identified in previous study through searching fish genome databases. herein, a transcript of irf11, ecirf11 was cloned from orange-spotted grouper, epinephelus coioides. the ecirf11 cdna sequence has 1573 bp in length, encoding a putative protein of 2 ... | 2017 | 27932023 |
| sensitive immunoassay-based detection of vibrio parahaemolyticus using capture and labeling particles in a stationary liquid phase lab-on-a-chip. | in the present study, a method was developed for detection of vibrio parahaemolyticus based on a stationary liquid phase lab-on-a-chip (slp loc). the present slp loc comprises a sample chamber, washing chamber, and detection chamber connected by two channels. the method utilizes two types of particles: capture particles (cps), which are magnetic nanoparticles functionalized with antibody; and labeling particles (lps), which are silica nanoparticles functionalized with horseradish peroxidase and ... | 2017 | 27923189 |
| lptd is a promising vaccine antigen and potential immunotherapeutic target for protection against vibrio species infection. | outer membrane proteins (omps) are unique to gram-negative bacteria. several features, including surface exposure, conservation among strains and ability to induce immune responses, make omps attractive targets for using as vaccine antigens and immunotherapeutics. lptd is an essential omp that mediates the final transport of lipopolysaccharide (lps) to outer leaflet. the protein in vibrio parahaemolyticus was identified to have immunogenicity in our previous report. in this study, broad distribu ... | 2016 | 27922123 |
| molecular identification and functional delineation of a glutathione reductase homolog from disk abalone (haliotis discus discus): insights as a potent player in host antioxidant defense. | glutathione reductase (gsr) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (gssg) into the reduced form (gsh). since the ratio between the two forms of glutathione (gsh/gssg) is important for the optimal function of gsh to act as an antioxidant against h2o2, the contribution of gsr as an enzymatic regulatory agent to maintain the proper ratio is essential. abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduct ... | 2017 | 27919756 |
| development of a more sensitive and specific chromogenic agar medium for the detection of vibrio parahaemolyticus and other vibrio species. | foodborne infections in the us caused by vibrio species have shown an upward trend. in the genus vibrio, v. parahaemolyticus is responsible for the majority of vibrio-associated infections. thus, accurate differentiation among vibrio spp. and detection of v. parahaemolyticus is critically important to ensure the safety of our food supply. although molecular techniques are increasingly common, culture-depending methods are still routinely done and they are considered standard methods in certain c ... | 2016 | 27911360 |
| fatty acid synthase plays a positive role in shrimp immune responses against vibrio parahaemolyticus infection. | fatty acid synthase (fas) is an important enzyme that catalyzes the synthesis of fatty acids. in this study, the role of the fas gene from pacific white shrimp litopenaeus vannamei (lvfas) in immune responses against vibrio parahaemolyticus infection was studied. the expression of lvfas could be up-regulated upon infection of v. parahaemolyticus and stimulation of lipopolysaccharide and poly (i:c). the promoter of lvfas was predicted to harbor a nf-κb binding site and dual-luciferase reporter as ... | 2017 | 27903451 |
| evaluation of medicinal plants and colloidal silver efficiency against vibrio parahaemolyticus infection in litopenaeus vannamei cultured at low salinity. | in shrimp aquaculture, reduction in the use of synthetic antibiotics is a priority due to the high incidence of resistant bacteria (vibrio) in the white shrimp litopenaeus vannamei. an increasing number of studies show bactericidal activity of natural treatments in aquaculture. the effectiveness of neem (azadirachta indica) and oregano (lippia berlandieri) aqueous extracts and colloidal silver against v. parahaemolyticus were evaluated in low salinity shrimp culture. results show that aqueous ex ... | 2016 | 27901504 |
| complete genome of vibrio parahaemolyticus forc014 isolated from the toothfish. | foodborne illness can occur due to various pathogenic bacteria such as staphylococcus aureus, escherichia coli and vibrio parahaemolyticus, and can cause severe gastroenteritis symptoms. in this study, we completed the genome sequence of a foodborne pathogen v. parahaemolyticus forc_014, which was isolated from suspected contaminated toothfish from south korea. additionally, we extended our knowledge of genomic characteristics of the forc_014 strain through comparative analysis using the complet ... | 2016 | 27891183 |
| case-control study of diarrheal disease etiology in individuals over 5 years in southwest china. | acute diarrhea is one of the major public health problems worldwide. most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in china. | 2016 | 27891182 |
| metabolic responses of haliotis diversicolor to vibrio parahaemolyticus infection. | vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone haliotis diversicolor. elucidation of metabolic mechanisms of abalones in responding to v. parahemolyticus infection is essential for controlling the epidemic. in this work, (1)h nmr-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of h. diver ... | 2017 | 27890800 |
| enhancing gilthead seabream immune status and protection against bacterial challenge by means of antigens derived from vibrio parahaemolyticus. | in an attempt to control the proliferation of the pathogenic bacterium vibrio parahaemolyticus in gilthead seabream (sparus aurata), the immunostimulant effect of lysate and toxa from this bacterium was evaluated. fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). afterwards, all fish specimens were experimentally infected with v. parahaemolyticus and mortality was recovered for 1 week. fis ... | 2017 | 27890799 |
| the role of ficolin-like protein (pcflp1) in the antibacterial immunity of red swamp crayfish (procambarus clarkii). | in invertebrates, ficolin-like proteins (flps) play important roles in innate immunity against pathogens. previous studies primarily investigated the functions of flps in immune recognition, activation, and regulation. however, limited research has examined the functions of flps as immune effectors. in this work, a ficolin-like protein was identified in red swam crayfish (procambarus clarkii) and designated as pcflp1. quantitative rt-pcr and western blot were employed to analyze the distribution ... | 2017 | 27888717 |
| hsp70 and hsp90 are involved in shrimp penaeus vannamei tolerance to ahpnd-causing strain of vibrio parahaemolyticus after non-lethal heat shock. | acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in farmed penaeid shrimp throughout asia. previous studies reported that a non-lethal heat shock (nlhs) could enhance disease tolerance in aquatic animals. here, we investigate whether the nlhs could enhance the survival of shrimp penaeusvannamei upon challenge with an ahpnd-causing strain of v. parahaemolyticus (vpahpnd). two nlhs conditions, acute an ... | 2017 | 27888131 |
| prevalence and factors associated with different pathogens of acute diarrhea in adults in beijing, china. | only a small proportion of patients with diarrhea are diagnosed with laboratory tests in china, and most are diagnosed based on clinical symptoms. therefore, understanding the prevalence of different diarrheal pathogens and their specific symptoms is important. | 2016 | 27886032 |
| biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries. | the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ... | 2017 | 27871750 |
| characterization of chemically-induced bacterial ghosts (bgs) using sodium hydroxide-induced vibrio parahaemolyticus ghosts (vpgs). | acellular bacterial ghosts (bgs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene e of bacteriophage phix174. in this study, vibrio parahaemolyticus ghosts (vpgs) were generated by chemically-induced lysis and the method is based on minimum inhibitory concentration (mic) of sodium hydroxide (naoh), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid, and sulfuric acid. the mic values of the respective chemicals w ... | 2016 | 27854308 |
| differential localization of chemotactic signaling arrays during the lifecycle of vibrio parahaemolyticus. | when encountering new environments or changes to their external milieu, bacteria use elaborate mechanisms to respond accordingly. here, we describe how vibrio parahaemolyticus coordinates two such mechanisms - differentiation and chemotaxis. v. parahaemolyticus differentiates between two distinct cell types: short rod-shaped swimmer cells and highly elongated swarmer cells. we show that the intracellular organization of chemotactic signaling arrays changes according to the differentiation state. ... | 2016 | 27853457 |
| non-cholera vibrios: the microbial barometer of climate change. | there is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. one particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. these gram-negative bacteria, including the species vibrio vulnificus, vibrio parahaemolyticus and vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambi ... | 2017 | 27843109 |
| season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea. | vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ... | 2017 | 27836844 |
| the hot oyster: levels of virulent vibrio parahaemolyticus strains in individual oysters. | vibrio parahaemolyticus is the leading cause of seafood-associated gastroenteritis and is most commonly transmitted by raw oysters. consequently, detection of virulent strains of this organism in oysters is a primary concern for seafood safety. vibrio parahaemolyticus levels were determined in 110 individual oysters harvested from two sampling sites in sc, usa. the majority of oysters (98%) contained low levels of presumptive v. parahaemolyticus however, two healthy oysters contained presumptive ... | 2017 | 27827805 |
| pi3k-akt signaling pathway is involved in hypoxia/thermal-induced immunosuppression of small abalone haliotis diversicolor. | the pi3k-akt signal pathway has been found to be involved in many important physiological and pathological processes of the innate immune system of vertebrates and invertebrates. in this study, the akt (hdakt) and pi3k (hdpi3k) gene of small abalone haliotis diversicolor were cloned and characterized for the important status of pi3k and akt protein in pi3k-akt signaling pathway. the full length cdnas of hdakt and hdpi3k are 2126 bp and 6052 bp respectively, encoding proteins of 479 amino acids a ... | 2016 | 27825946 |
| a c-type lectin (mrlec) with high expression in intestine is involved in innate immune response of macrobrachium rosenbergii. | c-type lectins (ctls) are pattern-recognition proteins that play an important role in innate immunity of vertebrates and invertebrates. in this study, a lectin cdna named mrlec was cloned and characterized from giant freshwater prawns (macrobrachiun rosenbergii). the full-length cdna of mrlec was 1431 bp, which contained an open reading frame of 1041 bp that encoded a protein with 346 amino acids. mrlec was found to contain a typical signal peptide of 18 amino acids and a single carbohydrate-rec ... | 2016 | 27818342 |
| regulation of the expression of iron-acquisition system genes in pathogenic vibrio species. | the genus vibrio includes >70 species, of which roughly a dozen cause vibriosis such as gastroenteritis, wound infections, and septicemia. most bacteria, including vibrio species, require iron for survival and growth. however, the bioavailability of iron is extremely low because it is usually present as an insoluble ferric complex in an aerobic environment or is bound to iron-binding proteins in mammalian hosts. therefore many bacteria have developed iron acquisition systems, including biosynthe ... | 2016 | 27803484 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus in the clam ruditapes philippinarum (adams & reeve, 1850) from emilia romagna and sardinia, italy. | marine vibrios, vibrio parahaemolyticus, v. vulnificus and v. cholerae are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. the aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the manila clam r. philippinarum from emilia romagna (er) and sardinia (sr) regions, italy. isolation was performed on chromagar(tm) vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) agar and m- ... | 2016 | 27800436 |
| differences in abundances of total vibrio spp., v. vulnificus, and v. parahaemolyticus in clams and oysters in north carolina. | filter feeding shellfish can concentrate pathogenic bacteria, including vibrio vulnificus and vibrio parahaemolyticus, as much as 100-fold from the overlying water. these shellfish, especially clams and oysters, are often consumed raw, providing a route of entry for concentrated doses of pathogenic bacteria into the human body. the numbers of foodborne infections with these microbes are increasing, and a better understanding of the conditions that might trigger elevated concentrations of these b ... | 2017 | 27793822 |
| draft genome sequences of one marine and one clinical vibrio parahaemolyticus strain, both isolated in sweden. | vibrio parahaemolyticus is the leading bacterial pathogen associated with seafood consumption. here, we report the draft genome sequences of one marine and one clinical strain, both isolated in sweden. these sequences will inform future comparative analysis of v. parahaemolyticus in northern europe. | 2016 | 27789643 |
| disulphide bond restrains the c-terminal region of thermostable direct hemolysin during folding to promote oligomerization. | pore-forming toxins (pfts) are typically produced as water-soluble monomers, which upon interacting with target cells assemble into transmembrane oligomeric pores. vibrio parahaemolyticus thermostable direct hemolysin (tdh) is an atypical pft that exists as a tetramer in solution, prior to membrane binding. the tdh structure highlights a core β-sandwich domain similar to those found in the eukaryotic actinoporin family of pfts. however, the tdh structure harbors an extended c-terminal region (ct ... | 2017 | 27784764 |
| in vivo activity and the transcriptional regulatory mechanism of the antimicrobial peptide sphyastatin in scylla paramamosain. | a new gene homologous to the reported antimicrobial peptide (amp) hyastatin from hyas araneus was screened in the ssh library constructed from the hemocytes of scylla paramamosain, and named sphyastatin. in vivo study showed that sphyastatin was predominantly expressed in hemocytes of s. paramamosain. with the challenge of either vibrio parahaemolyticus or lipopolysaccharide (lps), sphyastatin showed a positive response, meaning that it was probably involved in the immune reaction against bacter ... | 2016 | 27751917 |
| development of a highly sensitive lateral immunochromatographic assay for rapid detection of vibrio parahaemolyticus. | vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. it is also a foodborne pathogen that causes diarrhea in humans. the aim of this study is to develop an immunochromatographic lateral flow assay (lfa) for rapid detection of v. parahaemolyticus in both aquatic products and human feces of diarrheal patients. two monoclonal antibody (mab) pairs, ga1a-ic9 and ic9-kb4c, were developed and proven to be highly specific and sens ... | 2016 | 27751742 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| identification and function of a novel c1q domain-containing (c1qdc) protein in triangle-shell pearl mussel (hyriopsis cumingii). | c1q is the target recognition sequence of the classical complement pathway and a major link that connects innate and acquired immunity. in this study, a c1qdc homolog, hcc1qdc5, from the triangle-shell pearl mussel (hyriopsis cumingii) was identified. the complete nucleotide sequence of hcc1qdc5 cdna consists of a 5'-untranslated terminal region (utr) of 123 bp, a 3'-utr of 105 bp with a poly(a) tail, and an open reading frame (orf) of 1344 bp, which encodes a polypeptide of 447 amino acids. hcc ... | 2016 | 27725260 |
| quantification of vibrio species in oysters from the gulf of mexico with two procedures based on mpn and pcr. | oysters can accumulate potentially pathogenic water bacteria. the objective of this study was to compare two procedures to quantify vibrio species present in oysters to determine the most sensitive method. we analyzed oyster samples from the gulf of mexico, commercialized in mexico city. the samples were inoculated in tubes with alkaline peptone water (apw), based on three tubes and four dilutions (10(-1) to 10(-4)). from these tubes, the first quantification of vibrio species was performed (mos ... | 2016 | 27709461 |
| genome sequence of vibrio parahaemolyticus vp103 strain isolated from shrimp in malaysia. | 2016 | 27708636 | |
| expression patterns of two heat-shock cognate 70 genes during immune responses and larval development of the chinese mitten crab eriocheir sinensis. | two heat-shock protein (hsp) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as eshsc70-5 and eshsc70-3, respectively), were isolated from the chinese mitten crab eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adult crabs. the hsps exhibited 45-89% identity with other heat-shock proteins, and they shared similar structural features. eshsc70 mrna expressio ... | 2016 | 27706716 |
| dna shuffling approach for recombinant polyvalent ompas against v. alginolyticus and e. tarda infections. | molecular breeding via dna shuffling directs the evolution of vaccines with desired traits. in the present study, polyvalent ompa vaccines were generated by dna shuffling of five ompa genes from four species of bacteria vibrio parahaemolyticus, v. alginolyticus, edwardsiella tarda and escherichia coli. first, a new hybrid ompa was constructed using va0764 primers and used for construction of a prokaryotic expressing library pompas-fv containing 84 ompas, which were validated by pcr and sds/page. ... | 2016 | 27697557 |
| characterization of vibrio parahaemolyticus and its specific phage from shrimp pond in palk strait, south east coast of india. | phage therapy is an alternative and eco-friendly biocontrol agent to prevent and control multidrug resistant bacteria in the aquatic system. the aim of this study is to isolate and characterize the vibrio parahaemolyticus and its potential lytic phage from penaeus monodon growing-out by rearing in shrimp ponds in palk strait, south east coast of india. the conventional phenotypic characteristics and molecular identification was confirmed using 16s rrna sequence and to determine the antibiotic re ... | 2016 | 27697363 |
| colorimetric integrated pcr protocol for rapid detection of vibrio parahaemolyticus. | rapid detection of pathogens is of great significance for food safety and disease diagnosis. a new colorimetric method for rapid and easy detection of vibrio parahaemolyticus (v. parahaemolyticus or vp) has been developed in this research. a specific sequence was designed and integrated with the forward primer for molecular detection of vp. this specific sequence was tested and treated as the horseradish peroxidase (hrp)-mimicking dnazyme and could be amplified during the polymerase chain reacti ... | 2016 | 27690041 |
| genome sequence of vibrio parahaemolyticus vp152 strain isolated from penaeus indicus in malaysia. | 2016 | 27656174 | |
| genetic characterization of a blaveb-2-carrying plasmid in vibrio parahaemolyticus. | this report describes the first detection of a blaveb-2 gene in a vibrio parahaemolyticus strain isolated from a shrimp sample. the blaveb-2 gene was carried on a novel inc-type plasmid that was likely to have originated from aquatic organisms, as indicated by a comparison with other known genetic elements in the genbank database. however, the plasmid contains resistance elements usually harbored by members of the family enterobacteriaceae, suggesting that gene transfer events occurred and contr ... | 2016 | 27645248 |
| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| characteristics of vibrio parahaemolyticus isolates obtained from crayfish (procambarus clarkii) in freshwater. | vibrio parahaemolyticus usually occurs in coastal areas and is generally recognized as a marine bacterium. it has become the leading cause of gastroenteritis worldwide. in the present study, 96 v. parahaemolyticus isolates were obtained from freshwater crayfish (procambarus clarkii) and classified by multilocus sequence typing. fifty-three sequence types (sts) were identified among the 96 isolates analyzed, 38 of which were novel sts. these isolates fell into six groups and 42 singletons, sugges ... | 2016 | 27620824 |
| c-type lectin (mrctl) from the giant freshwater prawn macrobrachium rosenbergii participates in innate immunity. | c-type lectins (ctls) play important roles in the innate immunity of invertebrates. in this study, a novel ctl with a single carbohydrate recognition domain (crd) containing an epn (glu-pro-asn) motif was identified from the giant freshwater prawn macrobrachium rosenbergii. this ctl was designated as mrctl. the cdna of mrctl is 1788 bp with a 657 bp open reading frame that encodes a protein of 218 amino acids. the cdna and genome sequences of mrctl show a polymorphism that leads to mrctl isoform ... | 2016 | 27620819 |
| population analysis of clinical and environmental vibrio parahaemolyticus isolated from eastern provinces in china by removing the recombinant snps in the mlst loci. | vibrio parahaemolyticus is a common seafood-borne pathogenic bacterium which causes gastroenteritis in humans. continuous surveillance on the molecular characters of the clinical and environmental v. parahaemolyticus strains needs to be conducted for the epidemiological and genetic purposes. to generate a picture of the population distribution of v. parahaemolyticus in eastern china isolated from clinical cases of gastroenteritis and environmental samples, we investigated the genetic and evoluti ... | 2016 | 27608607 |
| impact of farm management on expression of early mortality syndrome/acute hepatopancreatic necrosis disease (ems/ahpnd) on penaeid shrimp farms in thailand. | asian shrimp farming industry has experienced massive production losses due to a disease caused by toxins of vibrio bacteria, known as early mortality syndrome/acute hepatopancreatic necrosis disease (ems/ahpnd) for the last 5 years. the disease can cause up to 100% cumulative pond mortality within a week. the objective of this study was to identify factors associated with ahpnd occurrence on shrimp farms. a case-control study was carried out on shrimp farms in four provinces of thailand. factor ... | 2017 | 27594170 |
| survival of foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce. | knowing the survival characteristics of foodborne pathogens in raw ready-to-eat (rte) seafood is the key to predicting whether they pose a microbiological hazard. the present study examined the survival of escherichia coli o157:h7, salmonella typhimurium, vibrio parahaemoliticus, listeria monocytogenes, and staphylococcus aureus in raw rte crab marinated in soy sauce. inoculated crabs (initial bacterial population=4.1-4.4logcfu/g) were immersed in soy sauce and then stored at refrigeration (5°c) ... | 2016 | 27591386 |
| draft genome sequence of pathogenic bacteria vibrio parahaemolyticus strain ba94c2, associated with acute hepatopancreatic necrosis disease isolate from south america. | vibrio parahaemolyticus is a pathogenic bacteria which has been associated to the early mortality syndrome (ems) also known as hepatopancreatic necrosis disease (ahpnd) causing high mortality in shrimp farms. pathogenic strains contain two homologous genes related to insecticidal toxin genes, pira and pirb, these toxin genes are located on a plasmid contained within the bacteria. genomic sequences have allowed the finding of two strains with a divergent structure related to the geographic region ... | 2016 | 27570736 |
| virulence of acute hepatopancreatic necrosis disease pirab-like relies on secreted proteins not on gene copy number. | to investigate the virulence of the vp_pirab-like genes in vibrio parahaemolyticus- acute hepatopancreatic necrosis disease (ahpnd)-causing strain and the factors that are associated with the virulence level. | 2016 | 27522063 |
| recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis. | anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, w ... | 2016 | 27517939 |
| unhealthy sugars in bacterial cytotoxicity. | bacterial type three secretion systems (t3sss) are central to virulence, but the processes stimulating their activation are unclear. in this issue of cell host & microbe, blondel et al. (2016) perform a genome-wide screen using crispr/cas technology to identify a role for cell surface sulfation and fucosylation in cytotoxicity mediated by vibrio parahaemolyticus t3sss. | 2016 | 27512898 |
| identification and characterization of an 18.4kda antimicrobial truncation from shrimp litopenaeus vannamei hemocyanin upon vibrio parahaemolyticus infection. | hemocyanin (hmc) is a multifunctional protein which plays many essential roles in invertebrate organism. recently more and more immune-related functions have been discovered on this protein. here the shrimp was infected with vibrio parahaemolyticus and the shrimp sera were analyzed by two-dimensional gel electrophoresis. totally 15 spots were identified as significantly up-regulated spots and further analyzed by maldi-tof/tof mass spectrometry (ms). four of them were identified as hmc derived tr ... | 2016 | 27506277 |
| shrimp with knockdown of lvsocs2, a negative feedback loop regulator of jak/stat pathway in litopenaeus vannamei, exhibit enhanced resistance against wssv. | jak/stat pathway is one of cytokine signaling pathways and mediates diversity immune responses to protect host from viral infection. in this study, lvsocs2, a member of suppressor of cytokine signaling (socs) families, has been cloned and identified from litopenaeus vannamei. the full length of lvsocs2 is 1601 bp, including an 1194 bp open reading frame (orf) coding for a putative protein of 397 amino acids with a calculated molecular weight of ∼42.3 kda. lvsocs2 expression was most abundant in ... | 2016 | 27497874 |
| evidences of abundant hemocyanin variants in shrimp litopenaeus vannamei. | hemocyanin (hmc) is a multifunctional immune molecule present in mollusks and arthropods and functions as an important antigen non-specific immune protein. our previous evidences demonstrated that litopenaeus vannamei hmc might display extensive molecular diversities. in this study, bioinformatics analysis showed dozens of variant sequences of the hmc subunit with higher molecular weight from l. vannamei (lvhmc). three variant fragments, named as lvhmcv1-3, which shared 85-99% nucleotide identit ... | 2016 | 27497189 |
| virulence and antibiotic and heavy metal resistance of vibrio parahaemolyticus isolated from crustaceans and shellfish in shanghai, china. | vibrio parahaemolyticus can cause serious human seafoodborne gastroenteritis and even death. in this study, we isolated and characterized 208 v. parahaemolyticus strains from 10 species of commonly consumed crustaceans and shellfish available in fish markets in shanghai, people's republic of china, in 2014. most of these aquatic species had not been detected previously. the results revealed an extremely low occurrence of pathogenic v. parahaemolyticus carrying the toxin gene trh (1.9%). however, ... | 2016 | 27497124 |
| the new antimicrobial peptide sphyastatin from the mud crab scylla paramamosain with multiple antimicrobial mechanisms and high effect on bacterial infection. | sphyastatin was first identified as a new cationic antimicrobial peptide in hemocytes of the mud crab scylla paramamosain. based on the amino acid sequences deduced, it was predicted that this peptide was composed of two different functional domains, a proline-rich domain (prd) and a cysteine-rich domain (crd). the recombinant product of sphyastatin displayed potent antimicrobial activities against the human pathogen staphylococcus aureus and the aquatic animal pathogens aeromonas hydrophila and ... | 2016 | 27493644 |
| leukocyte susceptibility and immune response against vibrio parahaemolyticus in totoaba macdonaldi. | vibrio parahaemolyticus is a serious pathogen that affects aquaculture. nonetheless, to the best of our knowledge, no studies have focused on its immunological implications in totoaba macdonaldi. thus, the early immune response to v. parahaemolyticus in juveniles of totoaba was studied at 24 h post-infection with an in vivo study. in addition, changes in cellular innate immune parameters - phagocytosis, respiratory burst activity and viability (annexin v/propidium iodide) - were evaluated in vit ... | 2016 | 27486683 |
| cloning of a big defensin gene and its response to vibrio parahaemolyticus challenge in the noble scallop chlamys nobilis (bivalve: pectinidae). | the noble scallop chlamys nobilis has been an important marine cultured bivalve in the southern sea of china for decades. however, large-scale mortality events often occurred during the scallop' cultivation. as one of amps (antimicrobial peptides), big defensin is an important component of the innate immunity against pathogenic microorganisms in invertebrates. in order to investigate whether the big defensin can play a role in the immune defense against pathogenic microorganisms in noble scallop ... | 2016 | 27474446 |
| development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease. | a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ... | 2016 | 27468405 |
| green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: siberian ginseng. | pharmacologically active stem of the oriental herbal adaptogen, siberian ginseng, was employed for the ecofriendly synthesis of siberian ginseng silver nanoparticles (sg-agnps) and siberian ginseng gold nanoparticles (sg-aunps). first, for metabolic characterization of the sample, liquid chromatography-tandem mass spectrometry analysis (indicated the presence of eleutherosides a and e), total phenol content, and total reducing sugar were analyzed. second, the water extract of the sample mediated ... | 2016 | 27468232 |
| vibrio parahaemolyticus and vibrio vulnificus in south america: water, seafood and human infections. | the bacterial species, vibrio parahaemolyticus and vibrio vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. they are concentrated by filter-feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. vibrio parahaemolyticus can cause abdominal cramping, nausea, diarrhoea, vomiting, chills and fever. vibrio vulnificus can ... | 2016 | 27459915 |
| isolation and identification of vibrio cholerae and vibrio parahaemolyticus from prawn (penaeus monodon) seafood: preservation strategies. | bacterial diseases are one of the major problems which affects the production, development and expansion of aqua culture. vibrio sp. are widespread in marine and estuarine environments. the several pathogenic species are commonly associated with outbreaks of vibrio species and it is mainly associated with food poisonings. in this research, the occurrence of vibrio sp. was studied by the isolation and it is confirmed by the biochemical methods. the growth rate was studied by changing the differen ... | 2016 | 27457973 |
| feasibility of using multiple-locus variable-number tandem-repeat analysis for epidemiology study of vibrio parahaemolyticus infections. | vibrio parahaemolyticus causes foodborne gastroenteritis, which is often associated with the consumption of raw or undercooked shellfish. molecular typing can provide critical information for detecting outbreaks and for source attribution. in this study, we describe the development and evaluation of an optimized multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) for the characterization of v. parahaemolyticus isolates. the discriminatory power of mlva was compared to that of pul ... | 2016 | 27454001 |