Publications

in vitro isothermal nucleic acid amplification assisted surface-enhanced raman spectroscopic (sers) for ultrasensitive detection of vibrio parahaemolyticus.	detection of pathogenic microorganisms is of great importance for public health and food safety. traditional protocols can hardly meet the continuously increased demanding in sensitivity and specificity of pathogen detections. in this study, we adopted vibrio parahaemolyticus (v. parahaemolyticus, vp) as the model analyte, and developed an antibody-vp-aptamer hetero-sandwich based surface-enhanced raman scattering (sers) method in conjunction with in vitro isothermal amplification for sensitive ...	2017	28832123
microencapsulation of essential thyme oil by spray drying and its antimicrobial evaluation against vibrio alginolyticus and vibrio parahaemolyticus.	the aims of this research were first, to evaluate the antibacterial potential of commercial thyme essential oil against v. alginolyticus and v. parahaemolyticys and second, using the spray drying technique to produce microcapsules. chemical compounds of thyme oil and microcapsules were identified and quantified being thymol the chemical component present at the highest concentration. oil-in-water (o/w) emulsions were prepared and the microcapsules were obtained with a spray dryer using maltodext ...	2017	28832836
microbiome dynamics in a shrimp grow-out pond with possible outbreak of acute hepatopancreatic necrosis disease.	acute hepatopancreatic necrosis disease (ahpnd) (formerly, early mortality syndrome) is a high-mortality-rate shrimp disease prevalent in shrimp farming areas. although ahpnd is known to be caused by pathogenic vibrio parahaemolyticus hosting the plasmid-related pirab(vp) toxin gene, the effects of disturbances in microbiome have not yet been studied. we took 62 samples from a grow-out pond during an ahpnd developing period from days 23 to 37 after stocking white postlarvae shrimp and sequenced ...	2017	28839269
inactivation of vibrio parahaemolyticus in shucked raw oyster ( grassostrea gigas) and clam ( venerupis phillippinarum) by using a combination of naclo and gamma irradiation.	this study investigated the synergistic effects of sodium hypochlorite (naclo) and gamma irradiation combination against vibrio parahaemolyticus in shucked oysters and clams. v. parahaemolyticus decreased to 1.1-5.6 log10 cfu/g in oysters and 1.1-5.7 log10cfu/g in clams by naclo (20-80 ppm) + γ irradiation (0.1-2.0 kgy) combinations. v. parahaemolyticus was not detected by 60 or 80 ppm naclo + 2.0 kgy. synergistic reduction of >1 log was observed by 60 ppm naclo + 0.3-2 kgy and 80 ppm naclo + 0. ...	2017	28840748
variation of genomic islands and flanking fragments in vibrio parahaemolyticus isolates from environmental and clinical sources in taiwan.	vibrio parahaemolyticus is a halophilic foodborne pathogenic bacterium that causes gastroenteritis; it has become an issue of global concern since the emergence and spread of pandemic o3:k6 strains. this study evaluated the role of vibrio pathogenicity island (vpai)-associated fragments in the genetic variation and grouping of this pathogen. distribution of some vpai fragments and flanking fragments (vpai-1, vpai-4, vpai-5, vpai-6 and vpai-7) was determined in a total of 53 v. parahaemolyticus i ...	2017	28841447
environmental determinants of vibrio parahaemolyticus in the chesapeake bay.	vibrio parahaemolyticus naturally-occurs in brackish and marine waters and is one of the leading causes of seafood-borne illness. previous work studying the ecology of v. parahaemolyticus is often limited in geographic extent and lacking a full range of environmental measures. this study used a unique, large dataset of surface water samples in the chesapeake bay (n=1,385) collected from 148 monitoring stations from 2007 to 2010. water was analyzed for over 20 environmental parameters with additi ...	2017	28842541
previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora.	a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ...	2017	28842846
whole-body microbiota of sea cucumber (apostichopus japonicus) from south korea for improved seafood management.	sea cucumber (apostichopus japonicus) is a popular seafood source in asia, including south korea, and its consumption has recently increased with recognition of its medicinal properties. however, because raw sea cucumber contains various microbes, its ingestion can cause foodborne illness. therefore, analysis of the microbiota in the whole body of sea cucumber can extend our understanding of foodborne illness caused by microorganisms and help to better manage products. we collected 40 sea cucumb ...	2017	28851208
environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic vibrio cholerae and vibrio parahaemolyticus strains.	with the overuse of antibiotics, many pathogens including vibrio cholerae and vibrio parahaemolyticus have evolved multidrug resistance making treatment more difficult. while understanding the mechanisms that underlie pathogenesis is crucial, knowledge of bacterial interactions of v. cholerae and v. parahaemolyticus could provide insight to their susceptibility outside of the human host. based on previous work showing competition among environmental strains, we predict that marine-derived bacter ...	2017	28857444