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endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium.the inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. endothelium-dependent responses were examined in vitro in normal yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (lad) 4 weeks before the study (n = 10). the presence of a complete endothelial lining was confirmed histologic ...19892766515
transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene.two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ...19892766934
serovars of vibrio parahaemolyticus.seashore water samples collected along the coastline in bulgaria and rumania contained in large numbers ok serovars of v. parahaemolyticus; some of these had been isolated repeatedly over an extended time period: 01 k32, 03 k30, 03 k48, 04 k37, 04 k53, 05 k17, 05 k30. the serovar 05 k17 was virtually present in all water samples and was also isolated from a case of purulent ear infection in a child from burgas. in contrast, strains recovered from asian and african coastal water had different k a ...19892768821
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...19892769548
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...19892769548
[complete occlusion both left main coronary artery and right coronary artery].a 67-year-old man, who had complete occlusion both of left main coronary artery (lmca) and right coronary artery (rca), underwent coronary revascularization. he had been suffering from severe angina pectoris preoperatively. exercise electrocardiogram (single master) showed myocardial ischemia. on cardiac catheterization, he showed extensive collaterals from right main coronary artery, and well-preserved left ventricular function. at operation, coronary arteries revealed severe sclerosis, especia ...19892770071
unexpected sex-ratios in families of language/learning-impaired children.there is a well-documented propensity of males affected with developmental language/learning impairment. results from this study demonstrate, unexpectedly, that this sex-ratio difference of males to females with developmental language/learning disorders was found to occur significantly only in families with a language/learning-impaired mother. in addition, a remarkably aberrant offspring sex-ratio was found in families of language/learning-impaired children who had an affected mother, but not fa ...20092771037
quest. nt's educational supplement. 20082771684
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...19892772364
[coronarography using high-flow 5f catheters. percutaneous technic using the femoral and brachial routes].in order to validate 5f catheters for assessing ischemic heart disease either by the femoral and the right brachial approaches, we prospectively studied with these catheters 125 patients by means of left ventriculogram and coronary artery angiograms. twenty-five patients were studied with pigtail and amplatz catheters using the right brachial approach (group i) and 100 patients were studied by the femoral route with pigtail and judkins catheters (group ii). results were compared to those obtaine ...20092772365
characterization of a non-structural 147-kda precursor polypeptide of echovirus 33 and its immunogenicity in man.zinc chloride (0.9 mm)--an inhibitor of the processing of the initial polypeptides of picornaviridae--was used to accumulate large precursors of echovirus 33 (ev33) and notably two proteins of 147- and 97-kda. polyclonal hyperimmune sera were raised in mice against these polypeptides and assayed by immunoblotting against ev33-infected cells blocked or not with zncl2, showing that protein 147--2abc3abcd according to the l434 convention--can be considered to be the precursor of the two non-structu ...19892772414
epiretinal membranes and cystoid macular edema in gyrate atrophy of the choroid and retina.a 26-year-old woman with gyrate atrophy of the choroid and retina and hyperornithinemia lost central visual acuity because of cystoid macular edema associated with epiretinal membrane formation. dietary manipulation over a 5-month interval reduced the plasma ornithine levels 37% but did not halt the progression of the gyrate atrophy nor influence the cystoid edema or visual acuity.20092772421
heterosexual spread of hiv infection. 19892772471
treatment of nasal polyps--medication or surgery and which technique. 20092772493
[prevalence of vibrio parahaemolyticus in seafood from restaurants in the city of mérida, yucatán].we report the prevalence of the vibrio parahaemolyticus in raw (11.54%), insufficiently cooked (0.00%) and partially cooked with heat (4.55%) sea foods, from restaurants of the city of merida, yucatan, mexico. we studied 190 samples from march to august 1987. we isolated 12 strains (6.32%) from an equal number of samples, whose biochemical characteristics corresponded with vibrio parahaemolyticus. we didn't find significant statistically differences when we compared the prevalences of the sample ...19892772734
single-dose intravenous h2 blocker prophylaxis against aspiration pneumonitis: assessment of drug concentration in gastric aspirate.this placebo-controlled trial compared the effects of preoperative, intravenous cimetidine (300 mg) or ranitidine (50 mg) on gastric ph and gastric volume in 31 adult patients requiring general anesthesia. the elapsed time from drug administration to initial gastric sampling did not differ significantly between ranitidine (45 minutes), cimetidine (48 minutes), or placebo (52 minutes) treated patients. ranitidine, but not cimetidine, significantly (p = 0.02) increased gastric ph when compared wit ...20092773628
comparative dna reassociation kinetics of cranes. 20092775167
comparative dna reassociation kinetics of cranes. 20092775167
primary structure of a novel 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits)-binding membrane protein highly expressed in torpedo californica electroplax.polyclonal rabbit antibodies were raised against 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits), an inhibitor of a variety of anion transport proteins. these antibodies specifically recognize sits-reacted erythrocyte band 3 in immunoprecipitations and western blots. in western blots of sits-reacted membrane proteins derived from vesicles of the electric organ of torpedo californica (known to express a sits-sensitive cl- channel) the antibodies recognized two major species of ap ...19892775201
isolation and identification of 3-deoxy-d-threo-hexulosonic acid as a constituent of the lipopolysaccharide of vibrio parahaemolyticus serotypes o7 and o12.treatment of the lipopolysaccharide of vibrio parahaemolyticus serotypes o7 and o12 with mild acid gave a compound which, on the basis of chemical analysis, g.l.c.-m.s., and 1h and 13c-n.m.r. spectroscopy, was characterized as 3-deoxy-d-threo-hexulosonic acid and shown to be present as a terminal non-substituted pyranose unit.19892776131
anomalous origin of right pulmonary artery from the ascending aorta.anomalous origin of one pulmonary artery from the ascending aorta without associated intracardiac defects is a rare congenital malformation. about 60 anatomic and surgical descriptions have been reported in the literature. up to 1974, 22 of the 50 reported cases underwent surgical correction with a 40% mortality rate. the natural history without surgery is poor with a very high mortality during the first year of life. successful anatomic correction of anomalous origin of right pulmonary artery f ...20092777879
production of monoclonal antibodies against thermostable direct hemolysin of vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay.a total nine hybridoma cell lines that produced monoclonal antibodies against thermostable direct hemolysin (vp-tdh), a possible pathogenic toxin, of kanagawa phenomenon-positive vibrio parahaemolyticus was isolated and characterized. these monoclonal antibodies (mabs) were divided into a minimum of five different specificity groups, including mabs specific to vp-tdh and common to vp-tdh and vp-trh, a vp-tdh-related hemolysin produced by kanagawa phenomenon-negative v. parahaemolyticus. an enzym ...19892779485
comparison of vibrio parahaemolyticus grown in estuarine water and rich medium.cell envelope composition and selected physiological traits of vibrio parahaemolyticus were studied in regard to the kanagawa phenomenon and growth conditions. cell envelopes were prepared from cells cultured in proteose peptone-beef extract (difco laboratories, detroit, mich.) medium or filtered estuarine water. protein, phospholipid, and lipopolysaccharide contents varied with culture conditions. the phospholipids present in the cell envelopes were identified as phosphatidylethanolamine, phosp ...19892782869
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...19892783611
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...19892783611
reversal of natural killing susceptibility in target cells expressing transfected class i hla genes.a number of studies have suggested that resistance of target cells to natural killing (nk) may be correlated with their level of expression of major histocompatibility complex (mhc) class i antigens. to examine this hypothesis directly, a nk-sensitive class i-deficient human b-cell line was transfected with mhc class i genes. the expression of transfected hla, but not h-2, class i gene products resulted in loss of susceptibility to human nk-mediated conjugation and cytolysis. furthermore, this p ...19892784569
the grading and prevalence of macular degeneration in chesapeake bay watermen.a new grading scheme was developed to classify the fundus features of macular degeneration. this scheme identifies the earliest fundus changes associated with macular degeneration, as well as specific drusen characteristics felt to be associated with an increased risk of developing the exudative forms of this disease. agreement between two graders using this scheme indicated good interobserver reliability. using this scheme, fundus photographs of 777 participants were graded in a population-base ...20092786408
myocardial reperfusion injury: an inflammatory reaction? 20092786416
induction of the insulin receptor and other differentiation markers by sodium butyrate in the burkitt lymphoma cell, raji.the very low expression of insulin receptors in the burkitt lymphoma cell raji was increased 2-fold, 6-fold and 10-fold after 1, 2 and 3 days, respectively, by incubation with the differentiation inducer sodium butyrate. insulin receptor number was increased without a change in receptor affinity, in association with an increase in the receptor alpha and beta subunits detected after cell-surface labelling and immunoprecipitation. expression of cell-surface class i and ii human leukocyte antigens, ...19892786417
human t lymphocytes possess a limited in vitro life span.the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ...19892786475
[the results of an investigation of the various factors influencing the late patency ratio of coronary bypass grafting].the purpose of this communication was to find out the factors influencing the late patency ratio of coronary artery bypass grafts of 42 patients in our institution. the following results were obtained: 1. the size of the measured diameters of coronary arteries and the graft flow rates were correlated well to the late patency ratio (p less than 0.05). 2. the regular physical exercise trainings were significantly important to keep patent grafts (p less than 0.01). 3. in blood serum studies, serum ...20092786538
[single photon emission computed tomography by using n-isopropyl-p-[123i]-iodoamphetamine and 99mtc-hexamethyl propyleneamine oxime in a patient with herpes simplex encephalitis].single photon emission computed tomographies (spects) by using n-isopropyl-p-[123i-imp]-iodo-amphetamine (123i-imp) and 99mtc-hexamethyl propyleneamine oxime (99mtc-hm-pao) were serially performed in a 70 yrs. old male with herpes simplex encephalitis (hse), and the chronological changes of accumulations were studied. accumulations of both 123i-imp and 99mtc-hm-pao increased initially, and then decreased with the stage of hse being advanced. the sequential appearance of the lesion in hse with 12 ...20092786584
comparison of the high-resolution structures of the alpha-amylase inhibitor tendamistat determined by nuclear magnetic resonance in solution and by x-ray diffraction in single crystals.the three-dimensional structure of the alpha-amylase inhibitor tendamistat determined by nuclear magnetic resonance in aqueous solution is compared with the tendamistat crystal structure refined at 2.0 a resolution. between the two independently obtained structures the root-mean-square distances are 1.05 a for the backbone atoms n, c alpha and c', 1.25 a for the backbone and the interior side-chains, and 1.84 a for all heavy atoms. these numbers show that the interior of the molecule is nearly i ...20092786963
wegener's granulomatosis (wg) and insulin dependent diabetes mellitus (iddm).we report a case of a 14 year-old girl with wegener's granulomatosis (wg) complicated by its direct brain invasion and insulin dependent diabetes mellitus (iddm). this case was diagnosed as wg early in its onset and successfully treated. as for iddm of this case, the presence of hla-dr4 and the islet cell surface antibody (icsa) was demonstrated. to our knowledge, this is the first case report on iddm associated with wg. we consider that wg may precipitate the development of iddm in a geneticall ...20092786968
effect of protein kinase c inhibitor (h-7) and calmodulin antagonist (w-7) on pertussis toxin-induced il-1 production by human adherent monocytes. comparison with lipopolysaccharide as a stimulator of il-1 production.human adherent monocytes stimulated with 1 microgram/ml pertussis toxin (pt) produced interleukin-1 (il-1), as measured by thymocyte co-stimulation assay and enzyme-linked immunosorbent assay (elisa), specific for il-1 alpha and il-1 beta. to clarify the role of protein kinase c (pkc) and calmodulin in il-1 production, we investigated the effects of a pkc inhibitor, h-7, and a calmodulin antagonist, w-7 on pt- and lipopolysaccharide (lps)-induced il-1 production by monocytes. addition of 10 micr ...19892787778
synergistic antitumor effects of interleukin 2 and the monoclonal lym-1 against human burkitt lymphoma cells in vitro and in vivo.interleukin 2 (il-2) regulates immune responses by inducing proliferation and differentiation of t-cells into cytotoxic cells, inducing lymphokine activated killer activity and enhancing antibody dependent cellular cytotoxicity (adcc). lym-1, a monoclonal antibody, recognizes a membrane antigen present on the surface of b-lymphoma cells and can be used for adcc. we therefore used raji (human burkitt lymphoma) cells to study the efficacy of combination therapy with il-2, lymphokine activated kill ...19892788499
effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure.previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ...19892789310
organ doses from cardiac and carotid digital subtraction angiography.estimates of mean organ doses from cardiac and carotid digital subtraction angiography (dsa) are obtained from measurements done using a rando-alderson tissue-equivalent phantom. thermoluminescent dosemeter chips and discs were calibrated and used for all measurements in the primary and scattered radiation fields. skin doses as well as mean doses received by the thyroid, lung, lens of the eye, breast, uterus and the ovaries were measured. a 30 degree right anterior oblique (rao) cardiac dsa stud ...20042790424
lorazepam as an adjunct in the treatment of auditory hallucinations in a schizophrenic patient. 20092794102
[an autopsy case of vibrio parahaemolyticus sepsis]. 19892794678
becker's melanosis and associated abnormalities. 20092798263
becker's melanosis and associated abnormalities. 20092798263
[pulmonary hydatidosis]. 20092799221
non-suppurative bacterial arthritis diagnosed by fine-needle aspiration biopsy. 19892799306
[obstruction of a naso-tracheal tube by an avulsed nasal concha].avulsion of a nasal concha by a nasotracheal tube obstructed the tube in a valvelike manner. the result was increased intrapulmonary pressure, hemodynamic instability, alveolar rupture, pneumomediastinum and pneumoperitoneum without pneumothorax. the case was successfully managed by removal of the nasotracheal tube and immediate oral reintubation.20052799996
[use of the long muscle of the neck for eliminating disorders of velopharyngeal function]. 20102800187
cardiac transplant recipients with preoperative pulmonary hypertension. evolution of pulmonary hemodynamics and surgical options.among 48 consecutive patients with pretransplant pulmonary vascular resistance (pvr) greater than 4 wood units, 38 patients underwent orthotopic heart replacement (oht), and the remaining 10 received a graft in a heterotopic position (hht). the oht recipients were smaller (63 vs. 73 kg, p less than 0.05) and received a larger donor heart (donor-recipient, 109% vs. 79%, p less than 0.001) with a shorter graft ischemic time (108 vs. 139 minutes, p less than 0.05) than hht recipients, reflecting pa ...20092805309
cardiac transplant recipients with preoperative pulmonary hypertension. evolution of pulmonary hemodynamics and surgical options.among 48 consecutive patients with pretransplant pulmonary vascular resistance (pvr) greater than 4 wood units, 38 patients underwent orthotopic heart replacement (oht), and the remaining 10 received a graft in a heterotopic position (hht). the oht recipients were smaller (63 vs. 73 kg, p less than 0.05) and received a larger donor heart (donor-recipient, 109% vs. 79%, p less than 0.001) with a shorter graft ischemic time (108 vs. 139 minutes, p less than 0.05) than hht recipients, reflecting pa ...20092805309
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. 19892806660
light and electronmicroscopic examination of calf lung tissue after left ventricular assisted circulation with the spindle pump.the influence of the spindle pump, a nonpulsatile blood pump, used as lvad on calf lung tissue was examined by means of the actual 16th protoype which was applied in 3 "acute" tests up to 13 hours of pumping duration and 8 prolonged experiments with pumping periods up to 63 hours. samples were taken during the operation and after exitus of the test animal and investigated by light and electronmicroscopy. histological findings of tissue changes could be ascribed to mechanical manipulations and sh ...19892807590
light and electronmicroscopic examination of calf lung tissue after left ventricular assisted circulation with the spindle pump.the influence of the spindle pump, a nonpulsatile blood pump, used as lvad on calf lung tissue was examined by means of the actual 16th protoype which was applied in 3 "acute" tests up to 13 hours of pumping duration and 8 prolonged experiments with pumping periods up to 63 hours. samples were taken during the operation and after exitus of the test animal and investigated by light and electronmicroscopy. histological findings of tissue changes could be ascribed to mechanical manipulations and sh ...19892807590
serological screening for a mucine-like carcinoma-associated antigen (mca) in patients with gastrointestinal tract malignancies.mouse monoclonal antibody b-12 identifies a high-molecular-mass glycoprotein antigen strongly expressed by breast carcinoma cells. since immunohistochemical studies have also demonstrated some reactivity with other epithelial neoplasms, and since "mucine-like carcinoma-associated antigen" (mca) has gained considerable interest as a tumor marker in breast cancer, we have investigated 34 patients with histologically proven advanced gastrointestinal malignancies for the presence of elevated mca ser ...19892808488
retrospect and prospect of electron microscopy in japan: biology/virology. 19982809471
the experience of screening mammography.nearly 1000 women who had just undergone screening mammography responded to a survey regarding demographics, the circumstances of the mammographic examination, and their responses to it. several findings of interest to the family physician include the following: (1) the majority of respondents obtained the examination as a result of their physician's referral rather than on their own. (2) most respondents experienced less pain during the procedure than they had anticipated. (3) the major express ...19982809522
when newborns die: do we practice what we preach? 20092809785
second meeting of the panamerican society for pigment cell research. bethesda, maryland, april 23-26, 1989. abstracts. 20092812206
second meeting of the panamerican society for pigment cell research. bethesda, maryland, april 23-26, 1989. abstracts. 20092812206
[surgical therapy of post-infarct aneurysm of the left ventricle. immediate and long-term results].between january 1982 and december 1986, 62 patients (59 male and 3 female) ranging in age from 37 to 69 years (mean 53) underwent resection of postinfarction left ventricular aneurysm. the indication for operation was angina in 23 cases (37%), congestive heart failure (chf) in 7 (11.2%), angina and chf in 29 (46.8%), rupture in 1; 2 patients were low symptomatic. ventricular arrhythmias were present in 6 (9.7%) cases and previous systemic embolism in 4 (6.4%). forty-seven (75.8%) patients had le ...20092812446
[surgical therapy of post-infarct aneurysm of the left ventricle. immediate and long-term results].between january 1982 and december 1986, 62 patients (59 male and 3 female) ranging in age from 37 to 69 years (mean 53) underwent resection of postinfarction left ventricular aneurysm. the indication for operation was angina in 23 cases (37%), congestive heart failure (chf) in 7 (11.2%), angina and chf in 29 (46.8%), rupture in 1; 2 patients were low symptomatic. ventricular arrhythmias were present in 6 (9.7%) cases and previous systemic embolism in 4 (6.4%). forty-seven (75.8%) patients had le ...20092812446
effects of blocked versus random practice by mentally retarded subjects on learning a novel skill.the purpose of this study was to assess the effects of different practice conditions on mentally retarded subjects learning a novel task. 20 subjects with a mean iq of 51.82 were randomly assigned to one of two practice conditions. the first group practiced at three different speeds (60, 30, and 15 rpm) on the pursuit rotor using a block arrangement in which each speed was practiced for five consecutive trials of 20 sec. duration before practicing at the next speed. the second group used random ...20102812988
effects of blocked versus random practice by mentally retarded subjects on learning a novel skill.the purpose of this study was to assess the effects of different practice conditions on mentally retarded subjects learning a novel task. 20 subjects with a mean iq of 51.82 were randomly assigned to one of two practice conditions. the first group practiced at three different speeds (60, 30, and 15 rpm) on the pursuit rotor using a block arrangement in which each speed was practiced for five consecutive trials of 20 sec. duration before practicing at the next speed. the second group used random ...20102812988
comparison of the gastrointestinal side effects of naproxen formulated as plain tablets, enteric-coated tablets, or enteric-coated granules in capsules.we studied the gastrointestinal side effects of three formulations of naproxen in 18 healthy male volunteers. in a latin-square design crossover study, the subjects received 500 mg naproxen twice daily for 7 days as plain tablets, enteric-coated tablets, or enteric-coated granules in capsules. the 51cr-edta absorption test was performed before and at the end of each drug period, to evaluate changes in the distal gut. the test dose was instilled distally in the duodenum to prevent lesions in the ...20102814364
an ultrastructural study of pigeon bursa of fabricius during involution.the ultrastructure of the pigeon bursa of fabricius during involution is described. according to the aa, the involutive process may be divided into 3 stages: an early involutive stage, a late involutive stage and a residual stage. the involutive process starts from epithelium later affecting the follicular medulla and all other structures of the organ. a mucoid degeneration can be observed, followed by an impressive fatty degeneration.20092817414
a photographic study of enamel defects among south australian school children.colour photographs were taken of the anterior teeth of 1757 consenting 8-9 year old primary school children in fluoridated and non-fluoridated areas of south australia. children with a lifelong exposure to fluoridated water were significantly more likely to have blemished permanent incisor teeth than the children who had never resided in a fluoridated area. children who resided in a non-fluoridated area but had taken fluoride supplements at some stage in their first five years had a prevalence o ...20092818303
[visualization of forensically important foreign bodies by direct roentgen magnification].the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...19892818477
[visualization of forensically important foreign bodies by direct roentgen magnification].the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...19892818477
reaction of tryptophanyl-trna synthetase from beef pancreas with periodate-oxidized atp.tryptophanyl-trna synthetase from beef pancreas reacts with periodate-oxidized atp according to biphasic kinetics. a rapid phase involves two groups of the protein, presumably lysine side-chains. the slow phase corresponds to the reaction of a larger number of groups. the time-course of the partial losses of the atp-ppi isotopic exchange and of the aminoacylation activities of the enzyme follow the labelling of the two fast-reacting groups. however, the ability of the enzyme to form a bis(trypto ...19872820730
formation of mboii vectors and cassettes using asymmetric mboii linkers.class-iis restriction endonucleases such as mboii cleave dna at a specified distance away from their recognition sequences. this feature was exploited to cleave dna at previously inaccessible locations by preparing special asymmetric linker/adapters containing the mboii recognition sequence. these could be joined to dna fragments and subsequently cleaved by mboii. attachment of a 3' phosphate to one of the two different oligodeoxynucleotides comprising the asymmetric duplex prevented ligation at ...19872820843
formation of mboii vectors and cassettes using asymmetric mboii linkers.class-iis restriction endonucleases such as mboii cleave dna at a specified distance away from their recognition sequences. this feature was exploited to cleave dna at previously inaccessible locations by preparing special asymmetric linker/adapters containing the mboii recognition sequence. these could be joined to dna fragments and subsequently cleaved by mboii. attachment of a 3' phosphate to one of the two different oligodeoxynucleotides comprising the asymmetric duplex prevented ligation at ...19872820843
type beta transforming growth factor affects adrenocortical cell-differentiated functions.type beta transforming growth factor (tgf-beta) had no detectable effect on mitogenic activities of bovine adrenocortical cells in culture. however, the presence of tgf-beta (1 ng/ml) in the medium resulted in a striking alteration of adrenocortical cell steroidogenic activities, maximally expressed after 18-20 h of treatment. tgf-treated cells exhibited a basal as well as an adrenocorticotropin-stimulated cortisol production inhibition by an average 50-60%, while camp accumulation in response t ...19872820972
type beta transforming growth factor affects adrenocortical cell-differentiated functions.type beta transforming growth factor (tgf-beta) had no detectable effect on mitogenic activities of bovine adrenocortical cells in culture. however, the presence of tgf-beta (1 ng/ml) in the medium resulted in a striking alteration of adrenocortical cell steroidogenic activities, maximally expressed after 18-20 h of treatment. tgf-treated cells exhibited a basal as well as an adrenocorticotropin-stimulated cortisol production inhibition by an average 50-60%, while camp accumulation in response t ...19872820972
characterization of a phosphoenzyme intermediate in the reaction of phosphoglycolate phosphatase.when 32p-glycolate and phosphoglycolate phosphatase from spinach are mixed, 32p is incorporated into acid precipitated protein. properties that relate the phosphorylation of the enzyme to the phosphatase are: the km value for p-glycolate is similar for protein phosphorylation and substrate hydrolysis; the 32p in the phosphoenzyme is diluted by unlabeled p-glycolate or the specific alternative substrate, ethyl-p; the activator cl- enhances the effectiveness of ethyl-p as a substrate and as an inh ...20092820973
characterization of a phosphoenzyme intermediate in the reaction of phosphoglycolate phosphatase.when 32p-glycolate and phosphoglycolate phosphatase from spinach are mixed, 32p is incorporated into acid precipitated protein. properties that relate the phosphorylation of the enzyme to the phosphatase are: the km value for p-glycolate is similar for protein phosphorylation and substrate hydrolysis; the 32p in the phosphoenzyme is diluted by unlabeled p-glycolate or the specific alternative substrate, ethyl-p; the activator cl- enhances the effectiveness of ethyl-p as a substrate and as an inh ...20092820973
oxidation-reduction reactions of cytochrome b6 in a liposome-incorporated cytochrome b6-f complex.the chloroplast cytochrome b6-f complex, incorporated into phospholipid vesicles, shows proton translocation with an observed h+/e- ratio of approximately 2. the oxidation-reduction behavior of cytochrome b6 during electron transport from duroquinol to plastocyanin is affected by incorporation. the most obvious effect of incorporation is an increase in the duration of a steady-state level of cytochrome b6 that persists during electron transport. reagents that decrease activity increase the durat ...20102821919
experimental transmission and pathogenesis of immunodeficiency syndrome in cats.we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ...19872823940
experimental transmission and pathogenesis of immunodeficiency syndrome in cats.we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ...19872823940
genetic and physical characterization of proba genes of the marine bacterium vibrio parahaemolyticus.intracellular proline pools have been implicated in the halotolerance of many organisms. to examine this relationship in a moderately halotolerant marine bacterium, vibrio parahaemolyticus, proline biosynthesis genes were cloned in various plasmids. some genetic and structural properties of those genes were examined. subcloning showed that about 3.1 kilobases of v. parahaemolyticus dna could complement proa and prob but not proc mutations of escherichia coli. the same fragment would also complem ...19872829719
properties of adenosine triphosphate-hydrolyzing enzymes in membrane vesicles of vibrio parahaemolyticus. 19872830036
mononuclear cell infiltration in central portions of human astrocytomas.the mononuclear cell infiltration in the central portion of human low- and high-grade astrocytomas has been examined using monoclonal antibodies to cell surface differentiation antigens. there was no significant difference between the number of t cells, b cells, or macrophages. the okt8+ (suppressor/cytotoxic) t cell population was the major lymphocyte subset in the infiltrates. there was, however, indirect evidence for a proportion of the total okt8+ cells being natural killer cells. overall, t ...19882830373
mononuclear cell infiltration in central portions of human astrocytomas.the mononuclear cell infiltration in the central portion of human low- and high-grade astrocytomas has been examined using monoclonal antibodies to cell surface differentiation antigens. there was no significant difference between the number of t cells, b cells, or macrophages. the okt8+ (suppressor/cytotoxic) t cell population was the major lymphocyte subset in the infiltrates. there was, however, indirect evidence for a proportion of the total okt8+ cells being natural killer cells. overall, t ...19882830373
prevalence of enteric pathogens in homosexual men with and without acquired immunodeficiency syndrome.we studied 388 homosexual or bisexual men from the baltimore-washington area to define the spectrum of enteric pathogen carriage in a population at high risk for "gay bowel syndrome" in association with human immunodeficiency virus infection. seventy-seven patients with acquired immunodeficiency syndrome, 68 gay men with symptoms of acute diarrhea or proctitis, and 243 gay men without gastrointestinal symptoms and participating in a natural history study of human immunodeficiency virus infection ...19882831107
a possible pain control system in a non-mammalian vertebrate (a lizard, gekko gecko).in a lizard (gekko gecko) the anterograde tracer pha-l was microiontophoretically applied to the predominantly serotonergic nucleus raphes inferior. extensive spinal projections from the rostral magnocellular part of this nucleus were demonstrated to the superficial layers of the dorsal horn and to the intermediate zone, more sparsely to the ventral horn. but, in addition, retrogradely labeled neurons were found in and just below a periventricular cell group in tegmentum mesencephali, i.e. the l ...20102831478
characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58.overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ...19882832379
primary signet-ring cell carcinoma of urinary bladder.a case of primary signet-ring cell carcinoma of the urinary bladder is described clinically and pathologically. in this rare neoplasm the characteristic cells contain large amounts of cytoplasmic mucin which compresses the nucleus to one side. this tumor demonstrates nicely the ability of bladder epithelium to undergo metaplasia. most previously described cases have pursued a relentlessly progressive and fatal course despite varied forms of therapy.20102834858
properties of the membrane-bound 5'-nucleotidase and utilization of extracellular atp in vibrio parahaemolyticus.vibrio parahaemolyticus utilized atp, adp or amp as the sole source of carbon. about three times higher activity of membrane-bound 5'-nucleotidase was observed in cells grown in the presence of these nucleotides than in their absence: and therefore the enzyme seems to be inducible. since the 5'-nucleotidase activity could be measured with whole cells, the active site of this enzyme appears to be outwardly oriented. both mg2+ and cl- were required for activity. among the divalent cations tested, ...19872835422
[an epidemic of diarrhea caused by vibrio parahaemolyticus occurring in abidjan in 1985].the authors report the epidemiological results of an epidemic about 84 choleriform syndrome in abidjan (ivory coast), june 1985.19872836102
human cytomegalovirus-associated dnase and the specific immune response in different clinical conditions. 19882837245
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...19882838023
[biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury]. 20102838873
[opioid peptides and their possible physiological significance]. 20092839888
[primary malignant tumors of the liver in childhood: ten-year experience at the national taiwan university hospital]. 20102840483
egg shell quality and cholecalciferol metabolism in aged laying hens.calcium-binding protein d28k (calbindin) synthesis, vitamin d metabolism and shell quality were investigated in young and aged laying hens fed diets containing either cholecalciferol (cc) or its 1-hydroxylated derivatives. duodenal calbindin concentration was similar in the young and in the aged laying hens. exogenous 1-hydroxylated cc derivatives increased duodenal calbindin concentration, regardless of age. shell weight and shell density (mg/cm2) were significantly lower (p less than 0.01) in ...19882841436
rna polymerase ii allows unwinding and rewinding of the dna and thus maintains a constant length of the transcription bubble.ternary complexes of rna-dna-rna polymerase ii, originating from the in vivo transcriptionally active sv40 minichromosomes, can be detected and analyzed by a method previously developed (choder, m., bratosin, s., and aloni, y. (1984) embo j. 3, 2929-2936). using this method, we compared the electrophoretic mobilities of sv40 ternary complexes with those of sv40 rna-dna complexes obtained after the removal of the polymerases. independent of the in vitro elongation of the nascent rna, topoisomers ...20092843504
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...19882844405
modulation of phosphoinositide hydrolysis by naf and aluminum in rat cortical slices.naf stimulated phosphoinositide hydrolysis in rat cortical slices. the production of [3h]inositol monophosphate was rapid for the first 15 min of incubation with naf, followed by a plateau. the major product detected was [3h]inositol monophosphate, although significant amounts of [3h]inositol bisphosphate and [3h]inositol trisphosphate were also produced. the stimulation of [3h]inositol monophosphate production by naf was concentration dependent between 2 and 20 mm naf. addition of 10 or 100 mic ...19882846782
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...19882847397
the potassium channel opening action of pinacidil; studies using biochemical, ion flux and microelectrode techniques.in rat aorta and rat portal vein, (-)- and (+)-pinacidil each produced a concentration-dependent inhibition of tension development. although the (-) isomer was the more potent, concentration effect curves for each isomer were steep with similar slopes. in rat portal vein, tetraethylammonium and procaine antagonised the relaxant effect of (+/-)-pinacidil, whereas 3,4-diamino-pyridine was without effect. intracellular microelectrode recording in rat portal vein showed that low concentrations of (+ ...19882848206
[99mtc-nanocolloid: clinical results of its use as a new inflammation marker].fifty scintigrams were performed on 45 patients using 99mtc-nanocolloid in order to test its affinity for inflammatory lesions; in these patients there was clinical and/or radiological suspicion of an inflammatory process. there was good correlation between the degree of uptake and the activity of the inflammatory process. specificity is limited because of uptake in certain tumours (eg. sarcomas of the extremities), but difficulties in differential diagnosis are not expected to be serious. judgi ...20082849165
99mtc dimercaptosuccinic acid (dmsa) scan as first investigation of urinary tract infection.a total of 115 children under 5 years who presented with a first symptomatic urinary tract infection and who had a 99mtc dimercaptosuccinic acid (dmsa) scan were studied to assess its value and compare the findings with those of other imaging techniques. renal cortical defects were detected in 65 kidneys by dmsa scan, intravenous urogram, and ultrasound scan combined; 62 (95%) being seen on dmsa scan. the finding of reflux on micturating cystourethrography showed a highly significant correlation ...20102849382
factors influencing the severity of escherichia coli and avian adenovirus group ii infections in chickens.chickens from lines selectively bred for either a high (hh) or low (ll) antibody response to sheep erythrocytes were challenged intravenously with avian adenovirus group ii (aa). spleen size was determined 6 days later. in some experiments the responses of chickens to aa and escherichia coli infections were compared. the level of corticosterone in the feed (15 mg/kg) which resulted in the lowest incidence of pericarditis in response to e. coli resulted in the greatest incidence of large spleens ...19882849405
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