Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cloning and expression of the 5'-nucleotidase gene of vibrio parahaemolyticus in escherichia coli and overproduction of the enzyme. | the gene encoding the membrane-bound 5'-nucleotidase of vibrio parahaemolyticus was cloned and expressed in escherichia coli. cells of e. coli harboring a plasmid, pnut5, which carries the 5'-nucleotidase gene were able to grow on atp as the sole source of carbon, although the original cells were not. the 5'-nucleotidase activity was detected in whole cells of e. coli harboring pnut5 and in membrane vesicles prepared from these cells. most properties of the 5'-nucleotidase produced in e. coli, t ... | 1989 | 2546929 |
| the blood-brain barrier and response of c.n.s. metastases to chemotherapy. | a case demonstrating a differential effect of chemotherapy on a pineal metastasis and parenchymal cerebral metastases is described. at presentation, extensive metastatic small cell carcinoma of the lung was present and ct scanning showed an apparently solitary metastasis in the pineal. the clinical course and serial ct scans showed significant improvement of the pineal tumor and simultaneous development of multiple intra-cerebral metastases. this case confirms that the pineal gland is excluded f ... | 2008 | 2547031 |
| hypoxic changes in hippocampal neurons. | 1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ... | 1989 | 2547034 |
| specificity of transposition of tn7 in vibrio parahaemolyticus. | tn7 was found to transpose at a high frequency from the plasmid, rp4::tn7, to the chromosome of vibrio parahaemolyticus. seven isolates carrying tn7 insertions were derived from three wild-type strains isolated from geographically distinct areas, and hindiii and bsteii dna digests of these strains were probed with a cole1::tn7 biotinylated probe. the results indicated that v. parahaemolyticus is similar to several other species which have been studied in having a highly preferred site of inserti ... | 1989 | 2550986 |
| binding modes of mammalian hepatic gal/galnac receptors. | mammalian gal/galnac receptors show dramatic preference for three-branched oligosaccharide structures over two- or one-branched counterparts. the spatial arrangement of the gal residues is extremely important for optimal binding. the three terminal gal residues in the best triantennary ligand are about 15-30 a apart, and therefore the sugar-combining sites on the receptor may also have the same geometry. the results obtained with synthetic ligands containing gal or galnac are in agreement with t ... | 2008 | 2551610 |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets. | the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ... | 1989 | 2556355 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated. | because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ... | 1989 | 2561041 |
| a role of carbohydrate-carbohydrate interaction in the process of specific cell recognition during embryogenesis and organogenesis: a preliminary note. | a possible role of cell surface glycoconjugates in cell recognition has been envisioned based on recognition of carbohydrates by cell surface proteins such as endogenous lectins, glycosyltransferases, and hydrolases (refs. 18-22 in text). a new possibility that a specific carbohydrate at the cell surface could be recognized by the same or similar carbohydrate on the counterpart cell surface is now suggested by specific interaction between lex and lex, but not between lex and sialylated or non-su ... | 2006 | 2563943 |
| effect of psoralens and ultraviolet radiation on murine dendritic epidermal cells. | monofunctional psoralens produce less phototoxicity than bifunctional psoralens after ultraviolet a (uva) irradiation. we investigated the effect of repetitive treatments with angelicin (isopsoralen), a monofunctional psoralen, plus uva radiation (ipuva) on the number and morphology of dendritic epidermal cells (dec). this effect was compared with that of 8-methoxypsoralen plus uva radiation (puva), uva alone, and uvb radiation. c3h/hen mice were treated topically with the drugs three times/wk f ... | 2008 | 2565931 |
| in-vivo effects of (e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine (mdl 72145) on amine oxidase activities in the rat. selective inhibition of semicarbazide-sensitive amine oxidase in vascular and brown adipose tissues. | one hour after mdl 72145 ((e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine) (2.5 mg kg-1) was given by intraperitoneal injection, the semicarbazide-sensitive amine oxidase (ssao) activity of rat aorta and brown adipose tissue measured in-vitro was reduced by more than 95% of its control value, whereas the monoamine oxidase (mao-a) activity remained virtually unaffected. the action of this drug on amine oxidases in the liver at this dose was less selective. the in-vitro effect of mdl 72145 on the ... | 1989 | 2565962 |
| effects of toxin isolated from the sea anemone bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes. | electrophysiological effects of a polypeptide toxin isolated from the sea anemone bolocera tuediae (bttx ii) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. nanomolar concentrations of bttx ii cause a concentration-dependent depolarization. this effect is prevented by the presence of tetrodotoxin. action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. the positive overshoot a ... | 1989 | 2566207 |
| genome type analysis of adenoviruses: isolates from one year from the hannover area. | adenoviruses (av), isolated from 138 children during the year 1981 in the hannover area, were studied by dna restriction analysis with the enzymes bamhi, bglii, bsteii, ecori, hindiii, kpni, and smai and compared with the respective prototypes. varying fragment patterns were depicted and genome types analyzed. prototype-like strains of av1 and 5 were not found. types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. altered restriction sites were physically mapped on the genom ... | 1989 | 2566302 |
| skin cancer among atom bomb survivors. | 2008 | 2566809 | |
| adherence targets of vibrio parahaemolyticus in human small intestines. | formalin-fixed human small intestinal mucosa with mucus coating, villi, and lymphoid follicle epithelium at the mucosal surface was used to test the adherence sites of clinically isolated (kanagawa phenomenon-positive) strains of vibrio parahaemolyticus. v. parahaemolyticus strains grown on cfa agar (supplemented with 3% nacl) for ca. 3 h at 37 degrees c possessed various levels of cell-associated hemagglutinins (has) which were detected with human or guinea pig erythrocytes. the observed adhere ... | 1989 | 2568344 |
| recombinant human erythropoietin treatment in patients on maintenance home haemodialysis. | 1989 | 2570273 | |
| campylobacter pylori, gastrin, acid secretion, and duodenal ulcers. | 1989 | 2570297 | |
| panic attacks, buspirone, and serotonin function. | 2008 | 2570299 | |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| effect of neuroleptics on positive and negative symptoms and the deficit state. | the concept of negative symptoms tries to operationalize a deficit syndrome observed in schizophrenia, but also in other disorders. the instruments for the measurement developed so far are unclear in their dimensional structure and validity. further methodological development is needed. a new scale for measuring negative symptoms was derived from the amdp-system and applied to results of drug trials with clozapine, fluperlapine, and haloperidol. the three drugs were equally effective on negative ... | 2008 | 2573105 |
| inositol 1,4,5-trisphosphate-induced calcium mobilization is localized in xenopus oocytes. | injection of inositol 1,4,5-trisphosphate (ins(1,4,5)p3) into the animal pole of xenopus oocytes induced membrane depolarization due to the internal mobilization of calcium, which activates a chloride conductance. repetitive injections of ins(1,4,5)p3 results in desensitization probably as a result of depletion of the internal store of calcium. desensitization was restricted to the region surrounding the site of injection. injection of ins(1,4,5)p3 at one position induced desensitization, which ... | 2008 | 2575752 |
| increased migration inhibition factor production by leukocytes of patients with herpes zoster given the leukocyte ultrafiltrate. | using the direct leukocyte migration inhibition assay, the cell-mediated immune response was followed in together 47 patients suffering from herpes zoster. of these, 19 persons were treated with partially purified and concentrated lysed leukocyte ultrafiltrate. enhancement and/or earlier production of the leukocyte migration inhibition factor--in the presence of varicella-zoster virus antigen--was observed by leukocytes from patients given the ultrafiltrate on days 2-3 since the onset of the ves ... | 1989 | 2576583 |
| the cardiovascular effect of intracerebroventricular endothelin in rats. | to investigate the cardiovascular action of endothelin within the central nervous system, we studied the effect of intracerebroventricular endothelin in conscious wistar rats. the endothelin increased blood pressure and the heart rate in a dose-related way. the increase in mean blood pressure produced by 100 ng/kg of endothelin (45 +/- 6 mmhg, mean +/- s.e.m., n = 10) was much greater than that produced by the same amount of intravenous endothelin (4 +/- 1 mmhg, n = 7). pretreatment with intrave ... | 1989 | 2576664 |
| effect of urapidil on the performance of ischaemic myocardium in anaesthetized dogs. | the effect of urapidil on the ischaemic myocardium was studied in eight anaesthetized dogs. stenosis of the left descending coronary artery reduced blood flow and systolic contraction of the post-stenotic myocardium by about 50%; the end-diastolic length of the post-stenotic myocardium and the end-diastolic pressure increased, while aortic pressure slightly decreased. subsequent administration of urapidil (0.25 + 0.25 + 0.5 + 1.0 mg/kg intravenously) did not affect the systolic shortening and en ... | 1989 | 2576670 |
| [results of synovectomy of the elbow joint in chronic polyarthritis]. | 2008 | 2576709 | |
| the need to reform indigent care. | 2008 | 2577825 | |
| [determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method]. | use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ... | 1985 | 2578835 |
| overexpressed pp60c-src can induce focus formation without complete transformation of nih 3t3 cells. | nih 3t3 cells were transfected with plasmids containing moloney murine leukemia virus long terminal repeats and either chicken c-src or v-src genes. in contrast with the effects observed after transfection with plasmids containing c-src and avian retrovirus or simian virus 40 promoter-enhancers (h. hanafusa, h. iba, t. takeya, and f. r. cross, p. 1-8, in g. f. vande woude, a. j. levine, w. c. topp, and j. d. watson, ed., cancer cells, vol. 2, 1984; h. iba, t. takeya, f. r. cross, t. hanafusa, an ... | 1985 | 2582237 |
| [nucleotide sequence of the b1-hordein gene of barley hordeum vulgare l]. | the gene encoding b1 hordein of hordeum vulgare (cv. donetsky 4) was cloned and entirely sequenced. it contains no introns and codes for of 293 amino acids long polypeptide with molecular weight 33418. our clone differs from the previously sequenced b1 hordein genes in some positions within the coding region (there are 4 nucleotide changes and a 12 bp deletion, as compared with the pb11 cdna clone, and 5 nucleotide changes, as compared with the pbhp184 genomic clone). these changes result in pol ... | 2008 | 2583483 |
| [phylogenetic analysis of genes of the influenza virus. relationship between adaptability and neutrality]. | detailed phylogenetic analysis of the gene family of hemagglutinin h3 of influenza a-type virus was fulfilled, taking into account the domain structure of protein and positions of antigen determinants. the densities of distribution of fixed synonimic replacements between domains ha1 and ha2 were shown to be actually equal (rho (ha1) = rho (ha2], and those of nonsynonimic ones to be unequal: their ratios were rho (ha1): rho (ha2) = 2.8 for nonepidemic branches, and rho (ha1): rho (ha2) = 7.7 for ... | 1989 | 2583491 |
| evidence of immunologic cross-reactivity between hemolysins of vibrio hollisae and vibrio parahaemolyticus demonstrated by monoclonal antibodies. | 1989 | 2584761 | |
| [a case of an s-shaped kidney complicated by calculosis and hydronephrosis]. | 2008 | 2585989 | |
| [a case of posttraumatic cerebral air embolism]. | we report a case of cerebral air embolism that occurred after chest trauma. the diagnosis was confirmed by ct and skull roentgenogram which demonstrated air bubbles in intracranial arteries. it is well recognized that cerebral air embolism is caused by various diagnostic and therapeutic procedures, and trauma. but it may often be overlooked because it is diagnosed clinically and there are no characteristic symptoms nor signs. although various ways of treatment have been reported, prevention and ... | 2008 | 2586716 |
| comparative amino acid sequence analysis of hemolysins produced by vibrio hollisae and vibrio parahaemolyticus. | vibrio hollisae produces a hemolysin (vh-rtdh) that is related to the thermostable direct hemolysin of vibrio parahaemolyticus (vp-tdh). although both hemolysins are essentially similar biologically and immunologically, they differ markedly in heat stability; vp-tdh is heat stable, whereas vh-rtdh is heat labile. to elucidate the relationships between their characteristics and molecular structures, we analyzed the amino acid sequence of vh-rtdh and compared it with that of vp-tdh. vh-rtdh consis ... | 1989 | 2592352 |
| urease-positive, kanagawa-negative vibrio parahaemolyticus from patients and the environment in the pacific northwest. | we previously reported the occurrence of vibrio parahaemolyticus in patients and the environment in the pacific northwest. the present studies compare the biochemical characteristics, kanagawa hemolysin reactions, and plasmid profiles of 13 patient and 221 environmental isolates of the organism. classical biochemical testing of the isolates revealed similar reactions for the clinical and environmental strains, and analysis in agarose gels revealed that 13 to 15% of the isolates had plasmids. the ... | 1989 | 2592543 |
| emergence of a restricted bioserovar of vibrio parahaemolyticus as the predominant cause of vibrio-associated gastroenteritis on the west coast of the united states and mexico. | examination of 45 human fecal isolates of vibrio parahaemolyticus revealed the emergence of an unusual bioserovar (o4:k12, urease positive) associated with cases of gastroenteritis which appear to be domestically acquired on the west coast of the united states and mexico. | 1989 | 2592553 |
| advances in oculoplastic surgery. | ophthalmic plastic surgery, a sub-specialty within ophthalmology, encompasses diagnosis and treatment of diseases and abnormalities of the eyelids, orbit, and lacrimal systems. eyelid carcinoma is a more common entity as well as blepharoptosis seen in children and adults. it can usually be corrected by surgery involving the levator palpebrae superioris or a tarsofrontalis suspension using synthetic material or human tissue. retraction of the upper eyelid, the opposite of blepharoptosis, may be s ... | 1989 | 2592933 |
| [state of the valvular function of the cardia in patients with duodenal ulcer before and after vagotomy and methods of its correction]. | the authors studied the valvular function of the cardia in 714 patients who were kept under regular medical observation and in 138 patients who had been operated on for duodenal ulcer. changes were found in the cardia of 9.4% of patients who underwent regular medical examination and in 17% of these subjected to operation. the valvular function of the cardia was impaired after vagotomy in all cases. fundoesophagocrurorhaphy is the best method for correction of cardiac dysfunction. | 2008 | 2593570 |
| exotic diarrhoeal problems and poliomyelitis. | exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death. | 1989 | 2594659 |
| identification of n1-acetylnorspermidine in vibrio parahaemolyticus and an enzyme activity responsible for its formation. | n1-acetylnorspermidine [ch3conh(ch2)3 nh(ch2)3nh3] was identified in vibrio parahaemolyticus, which contains norspermidine as a major polyamine. this is the first example for the natural occurrence of monoacetylated unusual polyamine. the n1-acetylnorspermidine content was the highest 4 h after inoculation. incubation of norspermidine and acetyl coa with a cell extract from v. parahaemolyticus produced n1-acetylnorspermidine. a remarkable increase in specific activity of the acetyltransferase wa ... | 1989 | 2599358 |
| a mutant hemolysin with lower biological activity produced by a mutant vibrio parahaemolyticus. | a mutant toxin (m-tdh) of thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus was isolated from the culture of a strain of this organism mutagenized with n-methyl-n'-nitro-n-nitrosoguanidine. although the m-tdh had a molecular structure similar to the native vp-tdh, the m-tdh retained only about 7% residual hemolytic activity of the native toxin. furthermore, other biological activities of m-tdh, such as lethality in mice and enterotoxicity in rabbit ileal loops, were also weakened ... | 1989 | 2599363 |
| alumina as stationary phase for ion chromatography and column-coupling techniques. | columns packed with alumina were combined with common anion-exchange columns and applied to the ion chromatographic determination of sulphate in brines and biological fluids and for the trace determination of iodide in mineral waters and fruit juice samples. alumina was found to be a highly selective stationary phase for the preconcentration of sulphate from complex matrices. further, owing to its selectivity, which is different from that of r4n+-type anion-exchange materials, it is well suited ... | 2008 | 2600151 |
| solving problems: down the yellow brick road. | 2008 | 2600651 | |
| symptoms associated with diarrhoeal illness at san lazaro hospital, manila in 1983 and 1984. | the clinical features associated with various agents of diarrhoeal disease were studied using 2,836 patients admitted to san lazaro hospital, manila. three general patient groups were considered including single pathogen isolations, "multiple pathogen" isolations, and "no pathogen" isolations. in general, symptoms of diarrhoeal illness were found to be non-specific. however, shigella flexneri. vibrio parahemolyticus, and rotavirus were significantly associated with a number of prominent symptoms ... | 1989 | 2609210 |
| [morphometric and histologic studies of the significance of the paraventricular nucleus for the thyroid gland axis after ganglionectomy and cold exposure with special consideration of the pineal gland]. | to analyse the role of the peripheral sympathetic fibres for the nucleus paraventricularis, the nuclear size and the neurosecretory material were studied 30 d after bilateral cervical ganglionectomy (gx). in order to examine the influence of gx at normal temperature (24 degrees c) and exposure to cold (10 degrees c), rats were subjected to a 72 h exposure to cold before killing. additionally thyroxine (t4), serum thyrotropin (tsh), and pituitary tsh were investigated. 4 times a day (light-dark c ... | 1989 | 2610374 |
| three dimensional observations of the palisade-shaped nerve endings of normal hair of rat's snout. | the nerve endings of normal hair of the rat's snout, partially digested with trypsin and hydrochloric acid, were studied by scanning electron microscopy. each lanceolate structure measured ca. 10 microns in length and was arranged around the hair follicle. these palisade-shaped nerve endings were situated almost beneath the sebaceous glands, ran upward, parallel to the axis of the hair follicle, and terminated in pointed shape. 2 kinds of cells, teloglia cell type i showing flat profile, and tel ... | 1989 | 2610376 |
| enhanced net mass transfer of hdl cholesteryl esters to apo b-containing lipoproteins in patients with peripheral vascular disease. | in vitro net mass transfer of hdl cholesteryl ester to apolipoprotein (apo) b-containing lipoproteins (hdl-cet) was found to be nearly twofold higher in plasma from 35 male patients with peripheral vascular disease compared to the values of 27 age- and sex-matched healthy controls (p less than 0.001). differences in hdl-cet were predominantly observed between normolipidemic patients and controls, and were also demonstrable in pairs of patients and controls with similar hdl cholesterol, vldl + ld ... | 2008 | 2612001 |
| impairment of modulating role of testosterone in growth hormone response to clonidine in essential hypertension. | to investigate the effects of endogenous testosterone (t) on the responsiveness of plasma growth hormone (gh) to central alpha 2 adrenergic stimulation, we administered clonidine (0.15 mg, p.o.) to 16 male patients with essential hypertension (eht) and 13 age-matched male normotensive controls (nt). although the basal t level was correlated with the peak gh increment (r = 0.709, p less than 0.01) and with the area under curve of gh (r = 0.714, p less than 0.01) in the nt group, no such correlati ... | 2008 | 2612023 |
| nasal mucosal temperature and the effect of acute infective rhinitis. | nasal mucosal temperature was measured in 71 healthy subjects with an electronic thermometer. no correlation was found between the nasal mucosal temperature and age or sex. an increased mucosal temperature was found in patients with acute rhinitis, an effect which is supposed to assist in the defence system against microorganisms. when measuring nasal mucosal temperature over a 7-h period at the same time as nasal airway resistance, there was no correlation between these factors, indicating that ... | 2008 | 2612033 |
| effects of postnatal dexamethasone treatment on development of alveoli in adult rats. | we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ... | 1989 | 2612450 |
| effects of postnatal dexamethasone treatment on development of alveoli in adult rats. | we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ... | 1989 | 2612450 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| two distinct areas of unequal crossingover within the steroid 21-hydroxylase genes produce absence of cyp21b. | we mapped crossover sites in chimeric, recombinant cyp21 genes from six patients with salt-losing congenital adrenal hyperplasia (cah). nucleotide sequences unique to the cyp21a pseudogene or to the active cyp21b gene were mapped using gene-specific restriction sites and oligonucleotide hybridizations. each chimeric cyp21 gene in the cyp21-deletion linked haplotypes contained sequences near the 5' end that were characteristic of cyp21a and only a single transition from sequences of cyp21a to tho ... | 2008 | 2613228 |
| prp (proline-rich protein) genes linked to markers es-12 (esterase-12), ea-10 (erythrocyte alloantigen), and loci on distal mouse chromosome 6. | the closely linked proline-rich protein (prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. they are part of a conserved linkage group that is represented on human chromosome 12p. two other markers, ea-10 and es-12, that were previously unassigned to a chromosome are closely linked to prp genes in the mouse. | 1989 | 2613230 |
| gene mapping by enzymatic amplification from flow-sorted chromosomes. | a new approach to gene mapping which combines enzymatic amplification with high-resolution flow sorting of human chromosomes has been devised. reliable amplification from as few as 200 chromosomes has been demonstrated. this method, with particular application to mapping the position of chromosomal translocations, has been used to show that the breakpoint for the constitutional translocation t(11;22)(q23;q11) lies proximal to the genes c-ets-1, thy-1, and t3 delta and distal to the int-2 gene. t ... | 1989 | 2613232 |
| production and characterization of a compound inhibitory to vibrio parahaemolyticus from proteus vulgaris. | the production and characteristics of a compound in proteus vulgaris g cultures which was capable of inhibiting vibrio parahaemolyticus and other food-borne pathogens was investigated. production was influenced by medium composition, ph and temperature but not by the extent of aeration. the compound was most inhibitory at the optimum temperature for growth of v. parahaemolyticus. the inhibitor was most stable at ph 7.0 and inhibition occurred even after heating at 70 degrees c for 30 min and aft ... | 1989 | 2613590 |
| in-vitro activity of dr-3355, an optically active isomer of ofloxacin, against bacterial pathogens associated with travellers' diarrhoea. | the in-vitro activity of dr-3355, the s-(-)-isomer of ofloxacin, was determined against bacterial pathogens associated with travellers' diarrhoea. dr-3355 was highly active against isolates of enteropathogenic escherichia coli (mic90 0.05 mg/l), salmonella spp. (mic90 0.10 mg/l), shigella spp. (mic90 0.10 mg/l), campylobacter jejuni (mic90 0.39 mg/l) and vibrio parahaemolyticus (mic90 0.39 mg/l). the activity of dr-3355 against these bacteria was generally two- to eight-fold greater than that of ... | 1989 | 2613604 |
| paraquat poisoning with acute renal failure--a case report. | paraquat poisoning is relatively rare and is associated with mortality varying from 35 to 50%. a patient who consumed paraquat developed features of non-oliguric acute renal failure and recovered following haemodialysis. renal biopsy done during the early recovery phase showed features of acute tubulo-interstitial nephritis with no disruption of tubular basement membrane. on recovery the patient had no evidence of proximal renal tubular dysfunction. | 2007 | 2613639 |
| standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera. | a non-competitive enzyme-linked immunoassay (elisa) has been standardized to supplement the in vivo potency test used for the quality control of inactivated tissue culture vaccines against rabies. the essentials of the elisa were: fixation of the virus in different dilutions of vaccine on the surface of microtitre plates; testing of the reference and up to six test vaccines on one plate; incubation with polyclonal antisera to rabies virus glycoprotein containing an excess of antibody; further in ... | 1989 | 2613705 |
| [clinical study of osteoporosis]. | 2008 | 2614196 | |
| palatal myoclonus associated with extremity tremor. | palatal myoclonus associated with extremity movements such as myoclonus or tremor is uncommon and reports are rare. five patients with palatal myoclonus and a rest tremor are presented. in four patients, a slow rest tremor (3 hz or less) was present. the tremor persisted on sustained posture and finger-to-nose maneuvers and was usually not synchronous with the palatal movements. it was not associated with clinical manifestations of parkinson's disease and occurred in conjunction with brain-stem ... | 2008 | 2614493 |
| acceptability of day care surgery. | although day case surgery is recommended, its widespread feasibility in an urban environment is unclear. we studied 100 consecutive unselected patients admitted for surgical procedures considered to be suitable for day case surgery. their age, fitness and social details were recorded and they were asked both before surgery and after discharge by a postal questionnaire, whether they would have preferred treatment as an outpatient. there were 47 men and 53 women with a mean age of 42.5 years. nine ... | 2008 | 2614766 |
| [the detection of vibrio parahaemolyticus and vibrio alginolyticus using modified culture media]. | studies have been completed for the detection of vibrio(v.) parahaemolyticus and v. alginolyticus in baltic sea fish. the author had prepared for that purpose a liquid culturing medium and a solid substrate. the percentage of positive findings is remarkable. the results so far recorded should be a good reason for further studies. | 1989 | 2619483 |
| [humeroscapular periarthritis]. | 2008 | 2620726 | |
| [humeroscapular periarthritis]. | 2008 | 2620726 | |
| haemolysins of vibrio parahaemolyticus strains passaged in mice. | some phenotypically non-haemolytic strains of v. parahaemolyticus were observed to produce haemolysins after two passages in mices. depending on the strain, both heat stable and heat labile haemolysins were induced. the heat stable haemolysin so induced, was immunologically different from the thermostable direct haemolysin produced by kanagawa positive strains of v. parahaemolyticus. | 1989 | 2620943 |
| "i listen--i forget: i read--i remember". information booklets for patients. | 2009 | 2621073 | |
| isolation of the saprophytic strain of mc-3 and participation of the cell surface structure in predation. | from a predatory bacterium, mc-3, a mutant strain which lost predation ability was isolated by chance selection. biological properties of the mutant were the same as the parent except only saprophytic property. properties of the parent and the mutant strains of mc-3, such as bacteriolytic activity of the culture supernatant, digestion of peptidoglycan of the host bacteria, and growth by utilizing the host cells or their cytoplasmic substances, suggested that cell surface structure of the host ce ... | 1989 | 2622396 |
| [study on 438 strains of pathogenic vibrio and their resistance to drugs]. | this paper reported the monitoring result of pathogenic vibrio from 1461 diarrhoea outpatients in haikou city from april to november 1986. 438 strains of pathogenic vibri (29.98%) had been identified. among them the vibrio parahemolyticus (14.99%) was more common and the next was non-0:1 v. cholerae strains (11.84%). the features of attack-time, age, sex and clinical symptoms etc. were discussed. | 1989 | 2624932 |
| effects of food preservatives and local anesthetics on synthesis of outer membrane proteins in vibrio parahaemolyticus. | the effects of seven food preservatives including salicylate, benzoate, dehydroacetate, sorbate and propionate, and two local anesthetics, phenethyl alcohol and procaine, on the synthesis of major outer membrane proteins in vibrio parahaemolyticus strain 3283-61 were investigated. the synthesis of the outer membrane proteins a, b, c and e was remarkably inhibited by all the food preservatives except propionate and two additional outer membrane proteins with respective molecular weights of about ... | 1989 | 2626751 |
| aging: the lived experience. | this exploratory study examined the lived experience of aging in a group of 32 community dwelling adults aged fifty to eighty years (m = 68.4). respondents completed in-depth interviews in which they described the meaning of aging, the "types of things" associated with aging in themselves and in others, and methods of coping with aging-related changes. respondents' overall impressions of the meaning of aging were generally positive; however, the changes they associated with aging, both in themse ... | 2008 | 2628349 |
| isolation and fundamental properties of a phospholipase a2 inhibitor from the blood plasma of trimeresurus flavoviridis. | phospholipase a2 inhibitor was purified from the blood plasma of habu, trimeresurus flavoviridis, by sephadex g-200 gel filtration, deae-cellulose chromatography, and blue-sepharose cl-6b column chromatography. the purified inhibitor was shown to be a glycoprotein with a molecular weight of about 100k. it was found to consist of four subunits whose molecular weights were around 20-24k. in order to examine the inhibition mechanism of the inhibitor, the interaction of the inhibitor with a phosphol ... | 1989 | 2628432 |
| maize phosphoenolpyruvate carboxylase involved in c4 photosynthesis: nucleotide sequence analysis of the 5' flanking region of the gene. | to clone the genomic dna fragment containing the putative promoter region of the gene for phosphoenolpyruvate carboxylase [ec 4.1.1.31] involved in c4 photosynthesis (c4-type pepc), maize genomic libraries were screened. on probing with a 384-bp fragment from the n-terminal coding region of the maize cdna for c4-type pepc, four ecori-fragments differing in the restriction map were cloned, reflecting the presence of a small gene family. southern blot analyses were carried out on the genomic dna a ... | 2008 | 2628434 |
| lennox-gastaut syndrome in association with tuberous sclerosis. | 2008 | 2630483 | |
| assessment of clinical application of test-dose concept for theophylline in patients with respiratory failure. | the possibility of clinical application of test-dose concept for theophylline was assessed in 11 patients with serious underlying disease who required theophylline. based on the pharmacokinetic parameters calculated from the single intravenous aminophylline administration, they received a continuous infusion of aminophylline in order to maintain about 10 micrograms/ml plasma which is considered to be the lowest therapeutic level. plasma levels during a constant rate infusion were assayed at 6:00 ... | 2008 | 2630628 |
| assessment of clinical application of test-dose concept for theophylline in patients with respiratory failure. | the possibility of clinical application of test-dose concept for theophylline was assessed in 11 patients with serious underlying disease who required theophylline. based on the pharmacokinetic parameters calculated from the single intravenous aminophylline administration, they received a continuous infusion of aminophylline in order to maintain about 10 micrograms/ml plasma which is considered to be the lowest therapeutic level. plasma levels during a constant rate infusion were assayed at 6:00 ... | 2008 | 2630628 |
| [motor complications in patients with parkinson disease treated with levodopa]. | motor complications appearing in 180 patients with parkinson's disease who received levodopa for a long period are reviewed. the most frequently observed motor disorders were wearing off fluctuations and dyskinesias of "dose benefit". most patients who presented a decrease in the response to levodopa developed signs during the course or findings in complementary tests which were incompatible with the diagnosis of parkinson's disease. in the present study, the duration of the treatment with levod ... | 2001 | 2631806 |
| [determination of antitoxoplasma igg antibodies using the elisa method]. | the described elisa reaction is suggested as a method for examination of antitoxoplasmatic igg antibodies. it is a simple, i.e. three-layer sandwich with the following layers: 1. antigen 2. the examined serum either in serial dilution or single dilution 1:300 and 3. a peroxidase anti-igg conjugate with a final conclusive step, i.e. a substrate solution with opd. sera and conjugate are allowed to bind for 30 mins. at 37 degrees c and the substrate solution with opd is allowed to act for 15 mins., ... | 2008 | 2632016 |
| [determination of antitoxoplasma igg antibodies using the elisa method]. | the described elisa reaction is suggested as a method for examination of antitoxoplasmatic igg antibodies. it is a simple, i.e. three-layer sandwich with the following layers: 1. antigen 2. the examined serum either in serial dilution or single dilution 1:300 and 3. a peroxidase anti-igg conjugate with a final conclusive step, i.e. a substrate solution with opd. sera and conjugate are allowed to bind for 30 mins. at 37 degrees c and the substrate solution with opd is allowed to act for 15 mins., ... | 2008 | 2632016 |
| formation of hydroxynorspermidine from exogenously added 1,3-diaminopropan-2-ol in vibrio species. | when vibrio parahaemolyticus aq 3627 was grown in the presence of 1,3-diaminopropan-2-ol (oh-dap), a new compound accumulated in the cells. this was identified as hydroxynorspermidine (oh-nspd), n-(3-aminopropyl)-1,3-diaminopropan-2-ol, by gas chromatography-mass spectrometry and thin-layer chromatography. it was also synthesized enzymatically from oh-dap by a cell-free preparation from this strain. all other vibrio strains examined also showed the ability to synthesize this compound, but none o ... | 1989 | 2632061 |
| a reticuloendothelial system-activating arabinoxylan from the bark of cinnamomum cassia. | a neutral polysaccharide, named cinnaman ax, was isolated from the dried bark of cinnamomum cassia blume. it was homogeneous on electrophoresis and gel chromatography. it is composed of l-arabinose: d-xylose in the molar ratio of 4:3, and its molecular weight was estimated to be about 1.0 x 10(6). methylation analysis, carbon-13 nuclear magnetic resonance and controlled smith degradation studies enabled elucidation of its structural features. it showed remarkable reticuloendothelial system-poten ... | 2008 | 2632069 |
| a reticuloendothelial system-activating arabinoxylan from the bark of cinnamomum cassia. | a neutral polysaccharide, named cinnaman ax, was isolated from the dried bark of cinnamomum cassia blume. it was homogeneous on electrophoresis and gel chromatography. it is composed of l-arabinose: d-xylose in the molar ratio of 4:3, and its molecular weight was estimated to be about 1.0 x 10(6). methylation analysis, carbon-13 nuclear magnetic resonance and controlled smith degradation studies enabled elucidation of its structural features. it showed remarkable reticuloendothelial system-poten ... | 2008 | 2632069 |
| studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1). | anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ... | 1989 | 2632089 |
| studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1). | anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ... | 1989 | 2632089 |
| [prognostication of the development of degenerative-dystrophic lesions of the hip joint in dysplasia]. | examined were 184 children with dysplasia of the both hip joints and initial forms of a degenerative-dystrophic lesion. the roentgenologic signs of dysplasia of the hip joint, indicating the possible development of the degenerative-dystrophic lesion were established. | 2009 | 2632942 |
| [the sympathetic and renin-angiotensin systems are complementary in blood pressure regulation]. | the consequences of a chronic destruction of sympathetic nerves or of the blockade of the renin angiotensin system (ras) on the blood pressure (bp) level and its spontaneous variability were studied using a computerized method which allows a continuous monitoring of bp in conscious unrestrained rats. guanethidine, used to lesion the sympathetic fibres did not alter the bp level but significantly enhanced its variability. on the opposite, angiotensin converting enzyme inhibition decreased the bp ... | 1989 | 2633862 |
| short chain fatty acid-induced colitis in mice. | short-chain fatty acids (e.g. acetic, propionic and butyric acid) are metabolic products produced by colonic bacteria. since intra-rectal instillation of dilute acetic acid to rats and cats has been reported to produce a diffuse colitis similar to the human disease, in our study we have investigated the effect of intra-rectal butyric acid in mice. male bka mice (n = 6) received 01 ml of dilute butyric acid by intra-rectal instillation which was flushed out with saline after 10 sec. control anima ... | 1989 | 2634625 |
| [measurement of orthodontic appliance force using prototype sensors]. | in introductory remarks the most important biomechanical principles, which are relevant for the research, and conditions which must be satisfied presented in intraoral measuring of orthodontic forces were pointed out. first the aim of the research was defined, and then the investigation of mechanical properties of acrylics as a material for orthodontic appliances were carried out. the type of strain gauges, bonds and measuring system configurations, as well as measuring amplifiers and recording ... | 2008 | 2635572 |
| in vitro cultures of trabecular meshwork cells of the human eye as a model system for the study of cellular aging. | cells from the human trabecular meshwork providing a drainage system for the outflow of aqueous humour in the eye were isolated and propagated in monolayer culture. following serial subcultivation of the primary cultures, there was a gradual decline in the fraction of dividing cells with increasing population doubling level (pdl) resulting finally in growth cessation and disintegration of these 'senescent' cultures. the number of population doublings was at most 20. senescent cultures revealed r ... | 1989 | 2640083 |
| [effect of two stage corticotomy on maxillary protraction]. | for the purpose of clarifying the effects of two stage corticotomy on maxillary protraction, experimental studies by using of 5 male japanese monkeys (macaca fuscata) were performed. results from cast analysis, roentgenographic cephalometry and histological examinations were as follows. i. in experimental groups that received two stage corticotomy with maxillary protraction, apical base of maxillary bone protruded markedly. ii. in control groups that maxillary protraction was applied without cor ... | 1989 | 2640929 |
| on presentation of the friedenwald award of the association for research in vision and ophthalmology to dr. gerald j. chader. | 2008 | 2643586 | |
| on presentation of the friedenwald award of the association for research in vision and ophthalmology to dr. gerald j. chader. | 2008 | 2643586 | |
| ketopantoyl-lactone reductase from candida parapsilosis: purification and characterization as a conjugated polyketone reductase. | ketopantoyl-lactone reductase (2-dehydropantoyl-lactone reductase, ec 1.1.1.168) was purified and crystallized from cells of candida parapsilosis ifo 0708. the enzyme was found to be homogeneous on ultracentrifugation, high-performance gel-permeation liquid chromatography and sds-polyacrylamide gel electrophoresis. the relative molecular mass of the native and sds-treated enzyme is approximately 40,000. the isoelectric point of the enzyme is 6.3. the enzyme was found to catalyze specifically the ... | 1989 | 2644973 |
| dexamethasone regulation of the expression of cytokine mrnas induced by interleukin-1 in the astrocytoma cell line u373mg. | bsf-2/il-6, gm-csf and il-1 beta mrnas were induced by recombinant il-1 in human astrocytoma cell line u373mg. the induction of bsf-2/il-6 and il-1 beta mrnas did not require de novo protein synthesis while that of gm-csf mrna required a newly synthesized protein. dexamethasone inhibited the induction of these cytokine mrnas by il-1. this process seems to require continued protein synthesis. these results suggest that the production of these cytokines are positively and negatively controlled by ... | 1989 | 2646146 |
| the difference between duchenne and becker dystrophies. | 2008 | 2648189 | |
| the difference between duchenne and becker dystrophies. | 2008 | 2648189 | |
| preventing nervous system sports injuries. | physical fitness and sports remain integral components of overall good health and a cornerstone of preventive medicine. when performed safely, under adequate supervision, and with appropriate protective gear, most of these activities are enjoyable, healthful and psychologically gratifying. when not performed safely by trained athletes, these same activities can be treacherous, injurious and permanently disabling. the goals of this article are to review and describe low-risk and high-risk sports ... | 2008 | 2648209 |
| preventing nervous system sports injuries. | physical fitness and sports remain integral components of overall good health and a cornerstone of preventive medicine. when performed safely, under adequate supervision, and with appropriate protective gear, most of these activities are enjoyable, healthful and psychologically gratifying. when not performed safely by trained athletes, these same activities can be treacherous, injurious and permanently disabling. the goals of this article are to review and describe low-risk and high-risk sports ... | 2008 | 2648209 |