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surgical aortic valvuloplasty using the cavitron ultrasonic surgical aspirator: an invasive hemodynamic follow-up study.valve repair and calcium debridement in patients with calcific aortic stenosis, using the cavitron ultrasonic surgical aspirator (cusa), results in a reduction in the aortic valve gradient while potentially avoiding long-term problems inherent to prosthetic valves. invasive followup data in these patients has not previously been reported. ten patients in whom cusa debridement was performed underwent cardiac catheterization prior to and 8.0 +/- 2.5 months following the procedure. compared to base ...20071913786
formycin a resistant mutants due to defect in adenosine transport system in vibrio parahaemolyticus.an antibiotic formycin a inhibited growth of vibrio parahaemolyticus under certain conditions, which suggested that formycin a was taken up by cells under these conditions. we found that formycin a was transported via the adenosine transport system which we previously reported as a na(+)-coupled cotransport system. we isolated many formycin a resistant mutants, and about half of them grew very poorly on adenosine as a sole source of carbon. judging from their reversion frequencies, these mutants ...19911914010
occurrence of 2-keto-3-deoxy-d-manno-octonic acid in lipopolysaccharides isolated from vibrio parahaemolyticus.the occurrence of 2-keto-3-deoxy-d-manno-octonic acid (kdo) in lipopolysaccharides (lps) of vibrio parahaemolyticus was demonstrated for the first time by gas chromatography-mass spectrometry after dephosphorylation, reduction, and methylation. kdo was virtually completely phosphorylated, since no kdo was detected by either gas chromatography or thiobarbituric acid assay before dephosphorylation. the level of kdo in all six strains of v. parahaemolyticus investigated ranged from 0.37 to 0.69%, w ...19911917863
optical sectioning of microbial biofilms.scanning confocal laser microscopy (sclm) was used to visualize fully hydrated microbial biofilms. the improved rejection of out-of-focus haze and the increased resolution of sclm made it preferable to conventional phase microscopy for the analysis of living biofilms. the extent of image improvement was dependent on the characteristics of individual biofilms and was most apparent when films were dispersed in three dimensions, when they were thick, and when they contained a high number of cells. ...19911917879
renal damage in mice after sequential cisplatin and irradiation: the influence of prior irradiation on platinum elimination.doses of 4-6 mg kg-1 c-ddp given 6 months before renal irradiation caused only a modest increase in functional radiation damage (def 1.1). these effects could be explained by additive toxicities and the damage was much less than when c-ddp was given 3-6 months after irradiation. pharmacokinetic studies did not demonstrate any decrease in the rate of platinum elimination after previous low-dose renal irradiation.20071924867
tryptophan 65 is essential for hemolytic activity of the thermostable direct hemolysin from vibrio parahaemolyticus.the effect of modification of aromatic and ionizable amino acid residues on the hemolytic activity of a thermostable direct hemolysin from vibrio parahaemolyticus was examined. tryptophan 65, one of the two tryptophan residues per subunit, was specifically modified with n-bromosuccinimide, resulting in complete loss of hemolytic activity. however, neither nitration with tetranitromethane of one of the nine tyrosine residues nor nlm-ethoxyformylation of two of the four histidine residues caused a ...19911926183
[age determination of venous thrombi by ultrasound].in addition to diagnosing deep-vein thrombosis of the lower limb and pelvis, determination of the age of the thrombus is of considerable importance in guiding treatment. the present study was done to find out whether thrombus age can be determined by ultrasound morphology. 138 examinations were carried out on 76 lower limbs with phlebographically demonstrated thrombosed veins. diameter of the thrombosed vein, echogenicity of the thrombus and demarcation of the venous wall from its surroundings w ...20071932731
acute amphetamine-induced subsensitivity of a10 dopamine autoreceptors in vitro.extracellular recordings were obtained from spontaneously active, presumed dopamine (da) neurons of the ventral tegmental area (vta) of the rat in a slice preparation. bath-applied (+)-amphetamine (amph) (1-30 microm) induced a concentration-dependent decrease in the firing rate of these neurons, which tended to saturate with the highest concentrations used (n = 11). this inhibitory effect was dependent on the activation of d2 receptors since it was reversed by the d2 antagonist sulpiride (n = 8 ...19911933376
measurement of sensitivity to adriamycin in hybrid spheroids.using the newly developed hybrid spheroid system, we have been able to measure sensitivities to adriamycin in two sublines of lovo cells (a human colon adenocarcinoma line). hybrid spheroids are composed of coagglomerated lovo cells, and nonproliferating hela "feeder" cells, mimicking a population of minitumors. spheroids of uniform size, but containing different numbers of lovo cells, were incubated with various concentrations of adriamycin and after washing, plated for colony formation. binomi ...19911933483
mammography quality assurance. 20071950889
percutaneous coronary and peripheral angioscopy with saline solution and carbon dioxide gas in porcine and canine arteries.to obtain a clear view with angioscopy, blood must be displaced. carbon dioxide (co2) gas has proved to be a safe intraarterial contrast agent, and it may have advantages over saline solution as an infusion medium for angioscopy. this study compared the use of co2 gas and saline solution in the femoral artery, in the presence and absence of a proximal occlusion, in nine pigs and six dogs. the applicability of co2 gas in the coronary arteries was also evaluated. in total 185 angioscopy procedures ...19911950994
lead use in california industry: its prevalence and health implications.an estimated 230,000 workers were reported to work in lead-using activities as a result of a 1986 statistical survey of employers in california. however, 68% of these workers worked in activities of relatively low hazard. this included 114,000 workers engaged in electronic soldering. over 1800 workers were reported to work in battery manufacture, a relatively high-hazard activity, but with consistent monitoring and protection. in contrast, over 4300 workers were involved in radiator repair activ ...20071951050
invasive thymoma presenting as aortic dissection.we report the case of an invasive thymoma presenting as an aortic dissection in a 49-year-old woman with hypertension and a marfanoid appearance. the patient presented to the emergency department with "tearing" substernal chest pain. a chest radiograph revealed a right para-aortic bulge consistent with a dissecting aorta, but an aortogram was normal. other disease states, including various types of mediastinal masses, have been reported to mimic aortic dissection.20051952311
stability of streptococcal pyrogenic exotoxin production with laboratory manipulation of group a streptococci.because of reported differences in the production of streptococcal pyrogenic exotoxins by group a strains associated with severe streptococcal infections, the stability of exotoxin production by specific strains was examined by passing group a streptococci on blood agar culture plates daily for 20 days. no changes were detected in either exotoxin genes or in exotoxin production during this time, suggesting that these reported differences are due to other explanations such as differences in the s ...19911955721
purification and characterization of a nuclease (3'-nucleotidase) from a penicillium sp.a nuclease (3'-nucleotidase) similar to p1 nuclease from penicillium citrinum was purified from a commercial digestive from a penicillium sp. the activity of the nuclease (pa) was separated to three fractions by diethylaminoethyl-toyopearl 650m column chromatography, in total yield of 10%. the apparent molecular weight of these three nucleases, pa1, pa2 and pa3 was 35000, 33000, and 32000, respectively. all of them were homogeneous so far as checked by sodium dodecyl sulfate slab gel electrophor ...19901964878
pili of a vibrio parahaemolyticus strain as a possible colonization factor.pili of vibrio parahaemolyticus were purified from a kanagawa phenomenon-positive strain (ha7) that belongs to serogroup o2:k3 and is adhesive to rabbit intestine. the organisms treated with the fab fraction of antipilus antibody failed to adhere to the intestine. purified pili had the ability to adhere to the intestine, but the pretreatment of the intestine with purified pili did not allow adherence of the organisms to the intestine. these results suggest that pili of this v. parahaemolyticus s ...19901967172
piliated vibrio parahaemolyticus adherent to human ureteral mucosa. 19901967625
pathogenic escherichia coli, yersinia enterocolitica, and vibrio parahaemolyticus. 19901977991
sequence analysis of nuta gene encoding membrane-bound cl(-)-dependent 5'-nucleotidase of vibrio parahaemolyticus.the membrane-bound 5'-nucleotidase of vibrio parahaemolyticus is unique in requiring cl- for activity. we cloned the nuta gene encoding the 5'-nucleotidase and sequenced it. it contained an open reading frame consisting of 1,680 nucleotides capable of encoding a protein of 560 amino acid residues. the first 21 amino acid residues of the n-terminal portion of this protein seem to be a signal peptide. the rest of the polypeptide (539 residues) is hydrophilic, and its molecular weight was calculate ...19912016269
characterization of a new thermostable direct haemolysin produced by a kanagawa-phenomenon-negative clinical isolate of vibrio parahaemolyticus.the production of two haemolysins, thermostable direct haemolysin (vp-tdh) and a vp-tdh-related haemolysin (vp-trh), by clinical isolates of vibrio parahaemolyticus has previously been reported. here we describe a third type of haemolysin (named vp-tdh/i), which is produced by a clinical isolate (strain th012) that is kanagawa phenomenon negative. vp-tdh/i was purified by a series of column chromatographies on deae-sephadex a25, hydroxyapatite, sepharose 4b and mono q. by physicochemical, biolog ...19912016584
on talc, tremolite, and tergiversation. ter-gi-ver-sate: 2: to use subterfuges. 20072039750
experimental liver cysts induced by 2-acetylaminofluorene.liver cyst formation was studied serially in an experimental model in which rats were fed a diet containing 0.02% 2-acetylaminofluorene, a carcinogen, for 6 weeks, followed by a normal diet for 42 weeks. cysts appeared in the portal tracts at the 12th week, by which time the intrahepatic bile ducts had also proliferated and dilated. some of the dilated bile ducts were cavernous or multicystic and appeared to represent a transitional form between the bile duct dilation and cystic formation. after ...19912040399
ovarian cancer. 20072042058
abrogation of dominant glucose intolerance in sjl mice by a growth hormone transgene.glucose tolerance was studied in transgenic mice (sjl x c57bl/6) expressing human gh under the control of a housekeeping promoter. parental sjl mice were found to harbour a dominant allele, termed here glid, determining glucose intolerance in pure-bred animals and in f1 hybrids with glucose-tolerant c57bl/6 mice. blood glucose levels in transgenic sjl x c57bl/6 hybrid mice were well controlled following glucose challenge, whereas non-transgenic hybrids failed to control their glucose adequately. ...19912043241
effect of macrophages on interleukin-2 (il-2)- and il-4-induced murine lymphokine-activated killer activity.the enhancing effect of macrophages on interleukin-2 (il-2)- and il-4-induced murine lymphokine-activated killer (lak) activity was investigated in this study. peritoneal macrophages significantly enhanced lak activity generated from accessory cell-depleted splenic lymphocytes in both il-2 and il-4 cultures. this effect was dependent on the number of macrophages and was not replaced by a factor derived from macrophages or lymphocytes. macrophages enhanced il-2- and il-4-induced lak activity agai ...20072045201
the economics of spaying and neutering: market forces and owners' values affecting pet population control. 19912045338
[the prevalence of vibrio parahaemolyticus and its antibodies in seafood handlers in mérida, yucatán].we report the prevalence of vibrio parahaemolyticus and of antibodies against vibrio parahaemolyticus in the feces and serum of fish and seafood handlers in the city of mérida, yucatán, mexico. between march 1 and august 31, 1989, we studied 81 feces samples and 81 serum samples from an equal number of handlers. vibrio parahaemolyticus was not isolated in any of the feces studied. we found no statistically significant differences upon comparing our zero per cent isolation in feces samples with t ...19912053021
an analysis of maximum mandibular movements, craniofacial relationships and temporomandibular joint awareness in children.this paper evaluates the relationship between maximum mandibular vertical opening and the following variables: chronological age, craniomandibular relationships, and temporomandibular joint awareness in children. the range of mandibular movement was evaluated in 189 children between the ages of 4 and 14 years, using the method of agerberg. measurements were found to be accurate and reliable only in the vertical plane. cephalometric tracings were made on 131 of the subjects. significant relations ...20072064068
[surveys on the contamination of marine fish with non-o1 vibrio cholerae and vibrio mimicus and food poisoning cases by these organisms].the present paper describes the relationship between the contamination with non-o1 vibrio cholerae and vibrio mimicus of marine fish, with special reference to the seasonal variation and the concentration of contamination, and the actual cases of domestic food poisoning by these organisms. a 10 year survey revealed that non-o1 vibrio cholerae (non-o1 v. cholerae) strains were frequently isolated from fish during the summer season with some variations from one year to another, and isolates from f ...19912066602
scalp cooling by cold air for the prevention of chemotherapy-induced alopecia.a new system is described for cooling the scalp with cold air in order to prevent chemotherapy-induced alopecia. compressed air was cooled by means of a vortex tube built into a hair-drier cap. this system reduced the blood flow in the scalp to 35%, the surface temperature to 14.2 degrees c and the intradermal temperature at hair follicle level to 29.2 degrees c. the low temperature could be kept constant for at least one hour of cooling. by means of comparison, with cryogel packs the lowest epi ...20072074916
[hyperoxalemia and secondary oxalosis of chronic renal insufficiency]. 20072074920
[a juvenile form of osteochondrosis]. 20072074966
serum fructosamine and lipid profile in type i diabetic children in turkey.serum levels of total cholesterol (tc), triglycerides (tg), lipoprotein cholesterol fractions (hdl-c and ldl-c) and fructosamine (fa) were determined in twenty-three children with type i diabetes and twenty healthy children aged 4-14 years. mean serum tc and hdl-c did not differ significantly between diabetic and nondiabetic children. mean serum tg and fa showed a statistically significant increase in the diabetic group compared to healthy children. it is interesting to note that extremely high ...19902075022
[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...19902075365
intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study.light and electron microscopy were used to analyze nine therapy-resistant and asymptomatic human periapical lesions, which were removed as block biopsies during surgical treatment of the affected teeth. the cases that required surgery represented about 10% of all of the cases which received endodontic treatment and root fillings during the period 1977 to 1984. these cases revealed periapical lesions when they were examined 4 to 10 yr after treatment. the biopsies were processed for correlated li ...19902094761
[the significance of the determination of catecholamines and their metabolites in neuroblastomas].a method for the determination of catecholamines and their metabolites in the urine was established. the specimens were previously cleaned by ion exchangers and the components were separated by a 'reversed phase' ionic pair chromatography, determined by an electrochemical 'wall-jet'-detector. this method was applied in order to find a diagnostic marker in case of neuroblastoma diseases. in 85 per cent of the patients (n = 13) we stated an increased ejection of dopamine.20062095056
[derivative spectroscopy and its use in sanitary hygiene control].an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ...20072099444
determination by a site-directed mutagenesis method of active site of thermostable direct hemolysin (tdh) produced by vibrio parahaemolyticus. 19902101139
etiological agents of diarrhoea.two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status.19902101388
properties of a hemolysin related to the thermostable direct hemolysin produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus.a hemolytic toxin (vp-trh) produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus was further characterized. the purified vp-trh showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (vp-tdh), a pathogenic toxin produced by kanagawa phenomen ...19902118824
photosystem ii characteristics of a constructed synechocystis 6803 mutant lacking synthesis of the d1 polypeptide.photosystem ii (psii) composition was studied in a mutant of the cyanobacterium synechosystis 6803 in which synthesis of the reaction center polypeptide d1 has been inactivated. the mutant thylakoids had lost also the other reaction center polypeptide d2 and the chlorophyll alpha-binding protein cp47. cytochrome b559 and the chlorophyll alpha-binding protein cp43 accumulated to almost wild-type amounts in mutant thylakoids. also the 33 kda polypeptide involved in water oxidation was present and ...19902129398
transfection of genes for cell surface products involved in antigen presentation--applications to the understanding of autoimmunity. 19852129493
structural and functional analysis of the first intron of the human alpha 2(i) collagen-encoding gene.the nucleotide (nt) sequence of the first intron of the human alpha 2(i) collagen-encoding gene (col1a2) has been determined from its 5' terminus (nt 207) to nt 2045 with respect to the transcription start point. although the first intron contains elements known to function in transcriptional regulation of other genes (two ap1-binding sites and an alternating gt stretch), comparison of this sequence with that of the mouse col1a2 first intron revealed a low degree of nt sequence identity and very ...19902129528
peripheral red blood cell survival invariance during pefloxacin treatment in subjects with glucose-6-phosphate dehydrogenase deficiency.in order to evaluate the possible damaging effect of pefloxacin on erythrocytes in subjects with g-6-pd deficiency, a trial on red-cell survival was carried out using a 51cr-labelled peripheral red blood cell technique. eight men, aged 52 to 64 years, with osteoarthrosis and g-6-pd deficiency (mediterranean type) were treated with 800 mg/day pefloxacin. pefloxacin did not modify red cell survival in the subjects under study. in all subjects erythrocyte gsh levels were investigated in baseline co ...19782130006
the use of diagnosis-related groups to highlight quality of care issues. 19702130809
yolk degradation in tick eggs: ii. evidence that cathepsin l-like proteinase is stored as a latent, acid-activable proenzyme.cathepsin l-like proteinase found in the eggs of the tick ornithodoros moubata is latent during embryogenesis, but can be activated by acid treatment. in crude extracts as well as in partially purified fractions, activation requires reducing conditions and is inhibited by leupeptin, which indicates that it is mediated by a thiol proteinase, probably by the cathepsin l itself. latency disappears in vivo at the time of the acute phase of yolk digestion, which takes place during late embryonic deve ...19902134179
[isolation of enteropathogenic vibrio in bivalves and mud from the nicoya gulf, costa rica].the presence of enteropathogenic vibrio was evaluated in 36 sediment samples and 41 bivalve samples obtained from 3 collecting sites in the golfo de nicoya, costa rica. isolation methods for halophilic and non halophilic vibrio were used. the biochemical profiles of the strains obtained revealed the presence of the following isolates: 224 vibrio parahaemolyticus, 3 v. furnissii, 1 v. damsela and 3 v. fluvialis. v. cholerae was not isolated, due principally to the use of tcbs agar.19902134726
characteristics of the h(+)-translocating adenosine triphosphatase of vibrio parahaemolyticus.we have characterized h(+)-translocating adenosine triphosphatase (atpase) in membrane vesicles of vibrio parahaemolyticus. the atpase required high concentrations (about 0.5 m) of na2so4 (or other salts) for its maximum activity. magnesium ion stimulated the atpase activity, but ca2+ did not. the activity of atpase was inhibited by tetrachlorosalicylanilide, an h+ conductor, but not by another h+ conductor, carbonylcyanide-m-chlorophenylhydrazone. the activity was strongly inhibited by dicycloh ...19902140074
[spectroscopy of the liver in vivo using nuclear magnetic resonance. a new approach in hepatic physiopathology].nuclear magnetic resonance (nmr) spectroscopy is a non-invasive means of detecting numerous compounds, thus enabling real-time studies to be carried out on such topics as energy metabolism, intracellular ph and main intermediate metabolism pathways. this method has been used for several years to study perfused livers and rat livers in vivo, but its application to man has just begun. we describe some of its principal uses, with emphasis on the possibilities it offers to investigate the metabolic ...19902140172
[antibiotic sensitivity of vibrio parahaemolyticus isolated in the turkmene ssr].sensitivity of 82 cultures of parahemolytic vibrios to 8 antibiotics was studied. it was shown that the majority of the strains were highly sensitive to levomycetin and gentamicin, sensitive to tetracycline, rifampicin, streptomycin, neomycin and kanamycin and resistant to ampicillin.19902141785
rapid purification and characterization of f1-atpase of vibrio parahaemolyticus.the f1 portion of h(+)-translocating atpase as purified from membrane vesicles of vibrio parahaemolyticus by a rapid procedure. the whole purification process (from culture of cells to purification of the enzyme) could be completed in 1 day. the f1-atpase consists of five subunits (alpha, beta, gamma, delta and epsilon) like f1 of escherichia coli and other microorganisms. the f1-atpase of v. parahaemolyticus showed some interesting properties. its activity was greatly stimulated by high concent ...19902142893
[lipoprotein (a)--a further risk factor in arteriosclerosis?].epidemiological studies have identified lipoprotein (a), which has been known since 1963, but has received attention only recently, as a further risk factor for premature arteriosclerosis. this substance is similar to low-density lipoprotein, but it's serum concentration (and thus the increase in risk) is genetically determined and highly variable from one individual to another. the usual dietary and drug measures have only little effect on lipoprotein (a) serum levels. although the physiologica ...20072147016
preservation of detached fragments in open fractures of the limbs.in open limb fractures, loss of bone fragments of considerable size, including portions of the joint surface, is a frequent finding. although the authors routinely use common surgical methods of reconstruction, in this paper they present a method for preserving these fragments by storage in the abdominal wall, and using them for autografting once healing of the soft tissues in the site of fracture has occurred. this method, illustrated by an analysis of 3 cases, appears to be simple and effectiv ...20062151600
[difficulties in the diagnosis of primary malignant histiocytoma of the lung].a case is presented of a round coin shadow seen on a routine chest radiogram producing grave diagnostic problems. during hospitalization the patient presented neurological and bone and joint involvement symptoms. transthoracic needle biopsy of the lesion allowed to diagnose large cell cancer, but autopsy changed this diagnosis to malignant histiocytoma.20072168043
modulation of experimentally induced epilepsy by intracerebral grafts of fetal gabaergic neurons.systemic administration of pilocarpine to rats induces seizures that resemble complex partial epilepsy in humans. susceptibility to these seizures is increased by lesion of the gabaergic striatonigral projection. transplantation of fetal gabaergic neurons, but also of control non-gabaergic tissue, to the deafferent substantia nigra can reduce such lesion-increased seizure susceptibility. these observations are consistent with prior evidence that gabaergic basal ganglia outflow plays an important ...19902168529
[brain stem auditory evoked potentials in the early period after a brain concussion].acoustic truncal evoked potentials (atep) were studied in 24 patients in the first 48 hours after concussion of the brain. the absolute peak latency of components 1, 3, and 5, the interpeak intervals 1-3, 3-5, and 1-5, and the interaural difference were determined. the results were compared with those of examination of a control group of 24 healthy individuals. individual and group appraisal failed to reveal any significant differences from normal values in none of the studied parameters in pati ...20072168645
[the topographic anatomical basis for ventriculosinusal shunting in hydrocephalus in nursing and young infants].the peculiarities in the structure and topographic anatomical relations of the superior longitudinal, lateral, and sigmoid sinuses were studied on block preparations of the posterior cranial fossa of 14 children under 3 years of age who died from somatic diseases. it was shown that intrasinus inclusions preventing catheterization of the sinus (bands and septa) were absent at the junction of the first angle of the sigmoid sinus with its descending part. the authors recommend this area for shuntin ...20072168650
mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells.we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ...19902168660
downregulation of parathyroid hormone receptors in renal membranes from aged rats.the mechanism of the inhibition or blunting of parathyroid hormone (pth)-stimulated na(+)-ca2+ exchange activity in renal cortical cells from aged rats was examined. the number of pth binding sites in basolateral membranes prepared from adult (6 mo) and old (24 mo) rats was quantitated by the binding of the synthetic analogue 125i-labeled [nle8,18, tyr34]bpth-(1-34) amide to the membrane. the maximum number of specific pth binding sites, bmax, was 92.7 +/- 9.3 and 36.7 +/- 6.1 fmol/mg protein, r ...19902168678
the dose rate effects on the in vitro radiolysis products of magnesium-guanosine-5'-monophosphate complexes in aqueous solutions.this study examined the effects on the radiolysis of magnesium-guanosine-5'-monophosphate complexes in deaerated aqueous solutions, investigated by fourier transform infrared (ft-ir) spectroscopy. it was found that when the system was irradiated with a dose rate of 500 rad/min (low dose rate), the oh radicals, h atoms and hydrated electrons (eaq-) produced from radiolysis of water reacted mainly by addition to the double bonds c4 = c5 and n7 = c8 of guanine. when the dose rate was 23,800 rad/min ...20072168727
[comparative evaluation of clinical and economic effectiveness of preventive therapy of affective and schizoaffective psychoses].the authors provide the results of a comparative study into the action of different drug approaches to the prevention of relapses of affective and schizoaffective psychoses with the aid of multifactorial consideration of different clinical, economic and socioeconomic indicators characterizing the "benefits and losses" because of the use of carbamazepine and lithium carbonate. the comparative analysis has shown a high normothymic activity of carbamazepine and lithium carbonate in the patients exa ...20072176049
from the national institute of allergy and infectious diseases. summary of the 25th united states-japan joint conference on cholera and related diarrheal diseases. 19902201739
migratory deficiency of clithon retropictus hemocytes to vibrio parahaemolyticus and escherichia coli.hemocytes of a marine gastropod, nerita albicilla, but not those of an estuarine gastropod, clithon retropictus, were observed to migrate to live and heat-killed cells of vibrio parahaemolyticus and escherichia coli through nucleopore membrane in blind well chamber. the defective migration of c. retropictus hemocytes might reflect, at least in part, the survival of v. parahaemolyticus in the estuarine gastropod.19902202845
[internal tooth resorption]. 20072206646
mucilaginous plants and their uses in medicine.throughout the world but especially in the tropical and subtropical zones, there are succulent and non-succulent plants which harbor readily releasable mucilage in their tissues, on the surface of their seeds or in their bark. this mucilage may have diverse practical uses. among these, it functions as a healing agent, casually or in the practice of traditional-folk or conventional medicine. the mucilage of some of these plants is well known to science and has been studied by pharmacologists and ...20082214811
water associated infections. 19902216140
[lung cancer in women in moscow (epidemiology, etiology and surgical treatment)].in the studied period (1973-1987) the lung carcinoma morbidity rate among females of moscow, in general intensive indices (per 100,000 females), showed a tendency to reduce (18.4 in 1973 and 15.1 in 1987). a reverse tendency is mostly encountered in other countries. more than half of patients with lung carcinoma were over 70 years of age. the ratio of males with lung carcinoma to that of females grew from 3.0:1.0 in 1973-1977 to 3.6:1.0 in 1983-1987. in the period from 1979 to 1988, 380 females ...20072223213
[lung cancer in women in moscow (epidemiology, etiology and surgical treatment)].in the studied period (1973-1987) the lung carcinoma morbidity rate among females of moscow, in general intensive indices (per 100,000 females), showed a tendency to reduce (18.4 in 1973 and 15.1 in 1987). a reverse tendency is mostly encountered in other countries. more than half of patients with lung carcinoma were over 70 years of age. the ratio of males with lung carcinoma to that of females grew from 3.0:1.0 in 1973-1977 to 3.6:1.0 in 1983-1987. in the period from 1979 to 1988, 380 females ...20072223213
[a congenital esophagotracheal fistula in an adult]. 20072223222
[a congenital esophagotracheal fistula in an adult]. 20072223222
[diagnosis and surgical treatment of traumatic damage to the sinus of valsalva following penetrating wounds of the heart].the authors had three patients with traumatic damage of the sinus of valsalva, caused by penetrating injury of the thorax inflicted with a knife. the patients were brought to the clinic in a grave condition with decompensation of systemic and pulmonary circulation. the anomaly called for emergency surgical correction under conditions of extracorporeal circulation. the diagnosis was based on the complaints, medical history, clinical picture, the results of functional methods of examination includ ...20072223226
[diagnosis and surgical treatment of traumatic damage to the sinus of valsalva following penetrating wounds of the heart].the authors had three patients with traumatic damage of the sinus of valsalva, caused by penetrating injury of the thorax inflicted with a knife. the patients were brought to the clinic in a grave condition with decompensation of systemic and pulmonary circulation. the anomaly called for emergency surgical correction under conditions of extracorporeal circulation. the diagnosis was based on the complaints, medical history, clinical picture, the results of functional methods of examination includ ...20072223226
the silent brain infarct before and after carotid surgery.routine computed tomography was prospectively performed pre- and postoperatively in 114 consecutive patients undergoing a total of 131 procedures on the carotid artery. hemispheric cerebral infarction was found preoperatively in three of the 16 hemispheres corresponding to symptomatic lesions (19%), in 18 of 109 hemispheres corresponding to transient ischemic attacks (16.5%), and in one of four hemispheres corresponding to lesions responsible for a fixed cerebral vascular accident (25%). two sur ...20072223547
the silent brain infarct before and after carotid surgery.routine computed tomography was prospectively performed pre- and postoperatively in 114 consecutive patients undergoing a total of 131 procedures on the carotid artery. hemispheric cerebral infarction was found preoperatively in three of the 16 hemispheres corresponding to symptomatic lesions (19%), in 18 of 109 hemispheres corresponding to transient ischemic attacks (16.5%), and in one of four hemispheres corresponding to lesions responsible for a fixed cerebral vascular accident (25%). two sur ...20072223547
expanded programme on immunization. neonatal tetanus survey. 20072223562
expanded programme on immunization. neonatal tetanus survey. 20072223562
injury prevention. cost of injury--a report to congress, 1989. 20072223563
injury prevention. cost of injury--a report to congress, 1989. 20072223563
fetal macrosomia in potential diabetics with normal oral glucose tolerance: a case control study. 20072223691
fetal macrosomia in potential diabetics with normal oral glucose tolerance: a case control study. 20072223691
cloning and expression of two genes encoding highly homologous hemolysins from a kanagawa phenomenon-positive vibrio parahaemolyticus t4750 strain.we have cloned and sequenced the gene encoding thermostable direct hemolysin (tdh), a possible virulence factor in vibrio parahaemolyticus gastroenteritis, from a kanagawa-phenomenon-positive strain, t4750. this strain was found to contain two sequences (tdha and tdhs) homologous to the tdh gene previously reported by nishibuchi and kaper [j. bacteriol 162 (1985) 558-564] and taniguchi et al. [microb. pathog. 1 (1986) 425-432]. sequence homology of the coding regior between tdha and tdhs was 97. ...19902227429
a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india.chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ...19902227713
a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india.chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ...19902227713
[growth and therapy in angle class ii/2--a longitudinal cephalometric study].the influence of both growth and functional therapy with the u-bow-activator type i on angle class ii subdivision 2 malocclusion is analysed in a cephalometric long term study. findings concerning skeletal problems: 1. therapeutically intended clockwise rotation of the mandible with vertically and sagittally controlled maxillary position, 2. achieving normal sagittal mandibular growth rates. dental problems are improved significantly. the results obtained by functional therapy and growth proved ...20072227736
[growth and therapy in angle class ii/2--a longitudinal cephalometric study].the influence of both growth and functional therapy with the u-bow-activator type i on angle class ii subdivision 2 malocclusion is analysed in a cephalometric long term study. findings concerning skeletal problems: 1. therapeutically intended clockwise rotation of the mandible with vertically and sagittally controlled maxillary position, 2. achieving normal sagittal mandibular growth rates. dental problems are improved significantly. the results obtained by functional therapy and growth proved ...20072227736
[initial attitudes to orthodontic treatment--the results of a practical and clinical questionnaire study (i)].312 patients from one clinic and nine offices, between eight and 15 years of age treated with bionators, their parents and a control group were questioned on various aspects of their treatment. findings regarding opinions about dentofacial appearance, initial motives and expectations are presented. aesthetic motives proved preponderant by seeking treatment though the children's ability to verbally differentiate aesthetic concepts was poor. above all, they expected visible treatment progress, abs ...20072227739
[initial attitudes to orthodontic treatment--the results of a practical and clinical questionnaire study (i)].312 patients from one clinic and nine offices, between eight and 15 years of age treated with bionators, their parents and a control group were questioned on various aspects of their treatment. findings regarding opinions about dentofacial appearance, initial motives and expectations are presented. aesthetic motives proved preponderant by seeking treatment though the children's ability to verbally differentiate aesthetic concepts was poor. above all, they expected visible treatment progress, abs ...20072227739
molecular epidemiologic evidence for association of thermostable direct hemolysin (tdh) and tdh-related hemolysin of vibrio parahaemolyticus with gastroenteritis.the kanagawa phenomenon induced by the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus is almost exclusively associated with clinical strains, and tdh has been considered an important virulence factor. however, kanagawa phenomenon-negative strains isolated from patients with diarrhea have recently been shown to produce tdh-related hemolysin (trh). we studied the distribution of the tdh gene encoding tdh and the trh gene encoding trh in vibrios by hybridization analyses. the presen ...19902228229
[development and application of enzyme-linked immunosorbent assay using monoclonal antibody against a hemolysin (vp-trh) of vibrio paraheamolyticus--evidence that vp-trh producing-kanagawa phenomenon-negative v. parahaemolyticus is a human pathogen].although it has been well established that kanagawa phenomenon-positive vibrio parahaemolyticus is a human enteropathogen, the kanagawa phenomenon-negative one has been considered to be probably not pathogenic. we have found, however, an outbreak of gastroenteritis due to kanagawa phenomenon-negative v. parahaemolyticus which produces a new toxin (vp-trh) resembling to vp-tdh, a responsible toxin of kanagawa phenomenon. in this study, we developed monoclonal antibodies against vp-trh which were ...19902230365
surface-induced swarmer cell differentiation of vibrio parahaemolyticus.vibrio parahaemolyticus distinguishes between life in a liquid environment and life on a surface. growth on a surface induces differentiation from a swimmer cell to a swarmer cell type. each cell type is adapted for locomotion under different circumstances. swimmer cells synthesize a single polar flagellum (fla) for movement in a liquid medium, and swarmer cells produce an additional distinct flagellar system, the lateral flagella (laf), for movement across a solid substratum, called swarming. r ...19902233248
[variations in frequency of cesarean sections in denmark].an investigation is presented on the basis of the medical register of births concerning all primiparae who were delivered of a liveborn singleton infant in 1982 and 1986. on the basis of the section on complications in the notification of the births, we have made a subdivision into four "approximate indications". we find a variation in frequencies of caesarean section from 9.7% (1982) to 17.1% (1986) between the various counties and an increase during the period investigated of 19%. 50% of the i ...20072238194
[variations in frequency of cesarean sections in denmark].an investigation is presented on the basis of the medical register of births concerning all primiparae who were delivered of a liveborn singleton infant in 1982 and 1986. on the basis of the section on complications in the notification of the births, we have made a subdivision into four "approximate indications". we find a variation in frequencies of caesarean section from 9.7% (1982) to 17.1% (1986) between the various counties and an increase during the period investigated of 19%. 50% of the i ...20072238194
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...19902238764
inspiring nursing students to mobilize communities in nepal.in 1987 nurse educators in nepal implemented a revised certificate nursing curriculum oriented toward primary health care (phc) and a bachelor of community health nursing (chn) curriculum with emphasis on leadership in phc. as both curricula included practical experience for nursing students in the community, the teachers had to strategize how to maximize both the students' learning and their contribution to community health. below, how the educators encouraged reluctant students to not only par ...20082249925
epidermal growth factor inhibits transiently the progression from g2-phase to mitosis: a receptor-mediated phenomenon in various cells.an immediate effect of epidermal growth factor (egf) on the cell cycle is described. egf, when given to replicating cells such as hela, a431, and d her 14, very rapidly inhibits the transition from g2 phase to mitosis (m) in a transient fashion. the influence of egf (10(-10) to 10(-7) m concentrations) on the g2-m transition of individual cells has been analyzed by time-lapse photography in cell lines carrying intact, mutated, or no egf receptor. the g2-m transition of cells devoid of egf recept ...20052253234
[insulin treatment of diabetes mellitus]. 20072258670
[insulin treatment of diabetes mellitus]. 20072258670
[clinical, bacteriological, and immunological examination and treatment of two papillon-lefevre syndrome patients].papillon-lefèvre syndrome (pls) is a rare disease accompanied by palmo-plantar hyperkeratosis and rapidly progressive periodontal breakdown of deciduous and permanent dentition. two unrelated female pls patients, four and seven years old, with severe periodontal destruction were examined. antibody titers against seven strains by the enzyme-linked immunosorbent assay (elisa), microbial cultures from deep periodontal pockets and mouth rinse samples and immunoblotting analysis were performed. titer ...19902258685
[clinical, bacteriological, and immunological examination and treatment of two papillon-lefevre syndrome patients].papillon-lefèvre syndrome (pls) is a rare disease accompanied by palmo-plantar hyperkeratosis and rapidly progressive periodontal breakdown of deciduous and permanent dentition. two unrelated female pls patients, four and seven years old, with severe periodontal destruction were examined. antibody titers against seven strains by the enzyme-linked immunosorbent assay (elisa), microbial cultures from deep periodontal pockets and mouth rinse samples and immunoblotting analysis were performed. titer ...19902258685
nacm, a cytopathogenic protein from naegleria gruberi, egs; purification, production of monoclonal antibody, and the immunoidentification of a product that develops in nacm-treated vertebrate cell cultures.the story of nacm involves the discovery of a deleterious response of cultured vertebrate cells to a component in cell-free lysates prepared from free-living amebae of the genus naegleria; hence the acronym nacm derived from naegleria ameba cytopathogenic material. the cellular reaction is the basis for the biological assay that has been fundamental in the study of the action of nacm in a variety of cell cultures. it also has been used in the determination of the physical characteristics, and to ...20082258825
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