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proceedings of the 3rd north sea conference on periodontology. maastricht, the netherlands, 16-19 may 1990. 20061679767
bone marrow involvement by non-hodgkin's lymphoma: the clinical significance of morphologic discordance between the lymph node and bone marrow. nebraska lymphoma study group.bone marrow specimens from 317 patients with non-hodgkin's lymphoma (nhl) obtained at initial staging were evaluated for the presence of lymphoma or benign lymphoid aggregates. thirty-two percent (102 patients) had lymphoma in their bone marrow, and 9% had benign lymphoid aggregates. bone marrow lymphoma was present in 39% of low-grade, 36% of intermediate-grade, and 18% of high-grade lymphomas. the bone marrow was involved in 25% of patients with diffuse large-cell or immunoblastic nhl (ie, dif ...20061694234
[a method of isolating vibrio parahaemolyticus from different substrates].a method for isolation of parahemolytic vibrios is suggested, based on their high mobility in semiliquid agar gel. a special semiliquid medium was designed and tried, containing agar-agar, peptone, sodium chloride, mannose, gelatin, indicator, progress agent. the material is inoculated into the center of a petri dish. after incubation at 37 degrees c parahemolytic vibrios form macrocolonies on the surface of the medium, colored black against the red background of the medium. this method permits ...19901695981
a simple polymerase chain reaction method for detection and cloning of low-abundance transcripts.a simplified polymerase chain reaction (pcr) technique for the detection, semiquantitation and cloning of low-abundance rnas is described. this assay involves first-strand cdna synthesis by reverse transcription of mrna with specific oligonucleotide primers, followed by second-strand synthesis and pcr amplification, in the same tube with only the addition of taqi dna polymerase. the assay is sufficiently sensitive to detect target rna from as little as 1 ng of total rna. the beta-actin transcrip ...19901698071
heterogeneity of distribution pattern at the electron microscopic level of heparan sulfate in various basement membranes.using the high-iron diamine thiocarbohydrazide silver proteinate (hid-tch-sp) staining technique and enzymatic digestion, we investigated the ultrastructural distribution pattern of heparan sulfate side chains of heparan sulfate proteoglycan (hspg) in various basement membranes (nerve, capillary, oral epithelial, muscle, and dental basement membranes). four different distribution patterns of stain deposits were identified as heparan sulfate on the basis of enzymatic degradation by heparitinase. ...19901698204
rating of the lesions in senile dementia of the alzheimer type: concordance between laboratories. a european multicenter study under the auspices of eurage.the study reported was intended to compare the impressions and analyses of investigators from 11 different laboratories on 2 slides, each from 6 cases with varying quantities of neuropathological change of the type found in alzheimer's disease and normal ageing. the material came from 6 selected female patients over 75 years of age all of whom had been examined in detail and assessed by the blessed test score. two were severely demented, 2 mildly demented and 2 were considered to be normal. unst ...20061698217
alpha-fetoprotein.the past year has seen major challenges to existing maternal serum alpha-fetoprotein testing protocols for both neural tube defects and chromosomal anomalies. these challenges are reviewed along with the physiology of alpha-fetoprotein; the use of amniocentesis, ultrasonography, and additional serum markers in women with elevated alpha-fetoprotein levels; and epidemiologic implications of maternal serum alpha-fetoprotein screening.20071717020
alpha-fetoprotein.the past year has seen major challenges to existing maternal serum alpha-fetoprotein testing protocols for both neural tube defects and chromosomal anomalies. these challenges are reviewed along with the physiology of alpha-fetoprotein; the use of amniocentesis, ultrasonography, and additional serum markers in women with elevated alpha-fetoprotein levels; and epidemiologic implications of maternal serum alpha-fetoprotein screening.20071717020
comparison of immunochemical specificities of vibrio parahaemolyticus o10 and o12 antigens using monoclonal antibodies.the monoclonal antibodies against vibrio parahaemolyticus o10 and o12 antigens (lipopolysaccharide, lps) were prepared and specificities of the antibodies were examined. five of six anti-o10 antibodies reacted with o10 antigen, but none of them reacted with o12 antigen. on the contrary all of the five anti-o12 antibodies reacted with o10 antigen as well as homologous o12 antigen. o10 and o12 antigens were subjected to alkali treatment or periodate oxidation, and reactivities of these chemically ...19911725552
[advances in the research of clinical use of recombinant human granulocyte colony-stimulating factors]. 19911726145
acute myocardial infarction after blunt chest trauma in young people: need for prompt intervention.we describe four cases of acute myocardial infarction in young patients, secondary to blunt chest trauma. one case was treated with intracoronary thrombolysis and angioplasty, two cases received systemic thrombolysis, and the last one did not have any reperfusion therapy. the coronary angiograms of the left anterior descending artery showed thrombosis in two cases, coronary dissection in one case, and no morphological lesions in the other. we encourage the early performance of angiographic studi ...20061764738
the value of an expert system in performing clinical drug trials.an expert system has been designed to assist the cardiologist in determining whether patients can be included in clinical trials. this system contains knowledge on inclusion and exclusion criteria for six drug trials, and has been validated in 100 randomly selected patients. in 97 cases, the expert system and the cardiologist made an identical classification; in the remaining three cases, the patient was incorrectly classified by the physician. the system will also optimize the order in which qu ...20061764928
analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development.using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ...19911769342
adherence of vibrio parahaemolyticus to rabbit intestinal epithelial cells in vitro.kanagawa positive strains of vibrio parahaemolyticus showed adherence whereas most of the kanagawa negative strains were non-adhering to rabbit intestinal epithelial cells. anti-hemolysin antisera did not inhibit the adherence of v. parahaemolyticus strains. moreover, the adhesive capacity of non-adhering strains was not enhanced by purified hemolysin. cell surface hydrophobicity remained the same in both kanagawa positive and negative strains of v. parahaemolyticus. fetuin strongly inhibited th ...19911769547
[transient polyuria in pregnancy in diabetes insipidus and gestational diabetes].two pregnant women developed overt polyuria (up to 11 l/day) and polydipsia during their second and third trimesters of pregnancy. in one patient hydronephrosis was present. both patients suffered from mild gestational diabetes mellitus. plasma sodium was 145 and 162 mmol/l. polyuria and urinary hypo-osmolality responded well to desmopressin acetate. after delivery, polyuria and polydipsia disappeared in one patient and significantly improved in the other. infusion of hypertonic saline one and t ...20071770904
minority student nurses' perceptions of their educational program. 20061773054
sequence and features of the tryptophan operon of vibrio parahemolyticus.the nucleotide sequence of the trp operon of the marine enteric bacterium vibrio parahemolyticus is presented. the gene order e, g, d, c(f), b, a is identical to that of other enterics. the structural genes of the operon are preceded by a long leader region encoding a 41-residue peptide containing five tryptophan residues. the organization of the leader region suggests that transcription of the operon is subject to attenuation control. the promoter-operator region of the v. parahemolyticus trp o ...19911773058
[effects of sodium chloride on destruction of microorganisms by microwave heating in potatoes].to assess the destructive effect of different sodium chloride concentrations (0, 0.3, 1.5, and 3%) on microorganisms with microwave heating, strains of vibrio parahaemolyticus, staphylococcus aureus, salmonella enteritidis, escherichia coli and bacillus cereus were used to inoculate a mashed potato preparation. after microwave heating for 1 min at 800 w, resulted in temperatures and rate of destruction of bacteria significantly higher (p less than 0.05) in the core than on the surface of mashed ...19911773068
mosaic trisomy 8 associated with jejunal duplication. 19911773546
[clinico-morphological signs of different forms of destructive pancreatitis].clinicomorphological comparisons in 434 cases of destructive pancreatitis showed that macroscopic signs of destruction develop in the first 12 hours from the onset of the attack. the elaborated criteria make it possible to evaluate the main morphological components of destructive pancreatitis--pancreonecrosis proper, parapancreatitis, peritonitis, and "polyvisceritis" and to use therapeutic measures on an individual basis.20061779556
illumination and errors in dispensing.the relationship between the level of illumination and the prescription-dispensing error rate in a high-volume army outpatient pharmacy was investigated. the prescription error rate was determined by direct, undisguised observation and retrospective prescription review under three levels of illumination (45, 102, and 146 foot-candles) during 21 consecutive weekdays. illumination was controlled in the prescription-checking area of the pharmacy by using additional fluorescent lamps and filters. th ...20071781468
[work capacity of man when using individual respiratory protective devices].gas-mask decreased the ability to work by 20-50%. it was caused by decreasing skin and respiratory evaporation. ventilation of the under mask interspace (90 1/min) was recommended.19911790865
[induction of lung maturation in the early stage of premature rupture of fetal membranes]. 20071790890
purification and characterization of a lecithin-dependent haemolysin from escherichia coli transformed by a vibrio parahaemolyticus gene.lecithin-dependent haemolysin (ldh) of vibrio parahaemolyticus was purified from escherichia coli c600 transformed with a plasmid (phl591) ligated with a 1.5 kb dna fragment of v. parahaemolyticus. the final preparation comprised two ldh proteins with different molecular masses which were immunologically cross-reactive and had the same enzymic activity. the ldh was a phospholipase hydrolysing both fatty acid esters of phospholipid, i.e. it hydrolysed phosphatidylcholine (pc) to lysophosphatidylc ...19911791426
synthesis, antimicrobial, and antitumor activity of a series of palladium(ii) mixed ligand complexes.mixed ligand complexes of cisdichloromethioninepalladium(ii) with 2-mercaptopyrimidine and 2-aminopyrimidine were synthesized and characterized by elemental analysis, conductivity data, infrared, and 1h nmr and 13c nmr spectra. in these mixed ligand complexes methionine coordinates to palladium through amino nitrogen and sulphur, thus leaving a free carboxylic acid group. the pyrimidine ligand coordinates to metal ion through n3. mixed ligand complexes of cisdichloroethioninepalladium(ii) with c ...19911791468
lauryl sulphate haemoglobin: a non-hazardous substitute for hicn in haemoglobinometry.the haemiglobincyanide (hicn) method for measuring haemoglobin is used extensively worldwide; its advantage is the ready availability of a stable and internationally accepted reference standard/calibrator. however, its use may create a problem, especially in automated analysers, as the waste disposal of large volumes of reagent containing cyanide may constitute a potential toxic hazard. as an alternative, conversion of haemoglobin to a sulphate derivative by nontoxic sodium lauryl sulphate has b ...20071794230
contribution of the tdh1 gene of kanagawa phenomenon-positive vibrio parahaemolyticus to production of extracellular thermostable direct hemolysin.kanagawa phenomenon-positive strains of vibrio parahaemolyticus contain two copies of the tdh gene (tdh 1 and tdh 2) encoding thermostable direct hemolysin (tdh). previous studies suggested that the tdh 2 gene, but not the tdh 1 gene, was responsible for production of extracellular tdh. in this study, a tdh 2-deficient isogenic mutant of kanagawa phenomenon-positive strain aq3815 was constructed by a suicide vector-mediated in vivo recombination method. the intact tdh 1 gene in the mutant contri ...19911795634
medical screening for occupational disease risk is not a control measure. 20061796975
medical screening for occupational disease risk is not a control measure. 20061796975
unconjugated and conjugated bilirubin pigments during perinatal development. iii. studies on serum of breast-fed and formula-fed neonates.the process of conjugation and secretion of bilirubin was studied in a group of healthy, full-term, exclusively breast-fed newborns and a control group of exclusively formula-fed infants by means of a reverse-phase high-performance liquid chromatographic analysis of bilirubins present in serum. the serum concentrations of unconjugated bilirubin, esterified bilirubin, and the proportion of diesterified bilirubin (as percent of esterified bilirubin) were not significantly different in breast- and ...19911797114
unconjugated and conjugated bilirubin pigments during perinatal development. iii. studies on serum of breast-fed and formula-fed neonates.the process of conjugation and secretion of bilirubin was studied in a group of healthy, full-term, exclusively breast-fed newborns and a control group of exclusively formula-fed infants by means of a reverse-phase high-performance liquid chromatographic analysis of bilirubins present in serum. the serum concentrations of unconjugated bilirubin, esterified bilirubin, and the proportion of diesterified bilirubin (as percent of esterified bilirubin) were not significantly different in breast- and ...19911797114
acute and chronic effects of parathion and 2,4 d on the oxygen consumption of chasmagnathus granulata (decapoda, brachyura).the effect of two pesticides widely used in argentina on the oxygen consumption of the estuarine crab chasmagnathus granulata was studied. constant pressure respirometers were employed to estimate the rate of oxygen consumption per weight unit of animals treated previously with each pesticide, both acute (96 h) and chronically (15 and 30 days). crabs exposed to parathion -an organophosphorate insecticide that causes the inhibition of acetylcholinesterase- show an increase of oxygen consumption a ...20061797200
acute and chronic effects of parathion and 2,4 d on the oxygen consumption of chasmagnathus granulata (decapoda, brachyura).the effect of two pesticides widely used in argentina on the oxygen consumption of the estuarine crab chasmagnathus granulata was studied. constant pressure respirometers were employed to estimate the rate of oxygen consumption per weight unit of animals treated previously with each pesticide, both acute (96 h) and chronically (15 and 30 days). crabs exposed to parathion -an organophosphorate insecticide that causes the inhibition of acetylcholinesterase- show an increase of oxygen consumption a ...20061797200
axillary artery thrombosis from handball. 20061797214
axillary artery thrombosis from handball. 20061797214
transcatheter management of pseudoaneurysms complicating pancreatitis.hemorrhage from rupture of an arterial pseudoaneurysm is a potentially fatal complication of pancreatitis. seventeen patients underwent transcatheter embolization of 23 arteries for the treatment of 20 arterial pseudoaneurysms secondary to pancreatitis. their records were reviewed retrospectively to evaluate the clinical benefit of transcatheter therapy. at presentation, 15 of the 17 patients had gastrointestinal, intrasplenic, retroperitoneal, intraperitoneal, or postoperative wound bleeding. t ...20061797219
transcatheter management of pseudoaneurysms complicating pancreatitis.hemorrhage from rupture of an arterial pseudoaneurysm is a potentially fatal complication of pancreatitis. seventeen patients underwent transcatheter embolization of 23 arteries for the treatment of 20 arterial pseudoaneurysms secondary to pancreatitis. their records were reviewed retrospectively to evaluate the clinical benefit of transcatheter therapy. at presentation, 15 of the 17 patients had gastrointestinal, intrasplenic, retroperitoneal, intraperitoneal, or postoperative wound bleeding. t ...20061797219
measurement of red blood cell antibodies in autoimmune hemolytic anemia.the enzyme-linked antiglobulin test (elat) was employed to measure the number of igg molecules per red blood cell (igg/rbc) in 11 patients with autoimmune hemolytic anemia (aiha). all patients with aiha had high levels of red cell-associated igg (110-3, 650 igg/rbc). the control group consisted of normal volunteers (n = 10) and patients with hereditary spherocytosis (n = 1), beta 0-thalassemia (n = 1), immunologic thrombocytopenic purpura (n = 3) and igg multiple myeloma (n = 4). all control ind ...20061797282
measurement of red blood cell antibodies in autoimmune hemolytic anemia.the enzyme-linked antiglobulin test (elat) was employed to measure the number of igg molecules per red blood cell (igg/rbc) in 11 patients with autoimmune hemolytic anemia (aiha). all patients with aiha had high levels of red cell-associated igg (110-3, 650 igg/rbc). the control group consisted of normal volunteers (n = 10) and patients with hereditary spherocytosis (n = 1), beta 0-thalassemia (n = 1), immunologic thrombocytopenic purpura (n = 3) and igg multiple myeloma (n = 4). all control ind ...20061797282
comparison of the electrophysiological effects of org 7797, disopyramide, mexiletine and propafenone in anaesthetized dogs with myocardial infarcts.1. the electrophysiological effects of intravenously administered org 7797 were compared with those of disopyramide (class ia), mexiletine (ib) and propafenone (ic) in anaesthetized dogs with 5-6 day-old left ventricular myocardial infarcts. 2. org 7797 (0.5 mg kg-1) slowed conduction at all levels of the myocardium as shown by increases in st-a, ah, hv and qrs intervals, very modestly prolonged atrial and ventricular refractory periods and slightly shortened ventricular repolarization. sinus no ...19911797309
comparison of the electrophysiological effects of org 7797, disopyramide, mexiletine and propafenone in anaesthetized dogs with myocardial infarcts.1. the electrophysiological effects of intravenously administered org 7797 were compared with those of disopyramide (class ia), mexiletine (ib) and propafenone (ic) in anaesthetized dogs with 5-6 day-old left ventricular myocardial infarcts. 2. org 7797 (0.5 mg kg-1) slowed conduction at all levels of the myocardium as shown by increases in st-a, ah, hv and qrs intervals, very modestly prolonged atrial and ventricular refractory periods and slightly shortened ventricular repolarization. sinus no ...19911797309
inbreeding in saguenay-lac-st-jean (quebec, canada): a study of catholic church dispensations 1842-1971.saguenay-lac-st-jean (slsj) is a rather geographically isolated region of quebec which shows a high occurrence of hereditary disorders. it has been suggested that high inbreeding might explain this situation. we studied the inbreeding in the slsj region by 10-year periods from 1842 till 1971 using the catholic church dispensations. the values of the mean inbreeding coefficient were found to be low during the whole period, reaching a peak of 22.94 x 10(-4) during the period 1902-1911. the values ...20071797631
purification and some properties of inducible lysine decarboxylase from vibrio parahaemolyticus.inducible lysine decarboxylase from vibrio parahaemolyticus aq 3627 was purified to apparent homogeneity and characterized. the enzyme displayed a molecular weight of 531000 by gel filtration and 79000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme required pyridoxal phosphate as a cofactor, and the ph optimum was 5.5. the km value for l-lysine was 3.2 mm, and the enzyme was inhibited by 6-aminocaproic acid and alpha-fluoromethyl analogs of cadaverine. delta-hydroxylysi ...19911799949
occult rupture of spleen. 20071800517
purification and characterization of a hemolysin of vibrio mimicus that relates to the thermostable direct hemolysin of vibrio parahaemolyticus.a hemolysin (designated vm-rtdh) from vibrio mimicus (aq0915-e13) was purified by ammonium sulfate fractionation and successive column chromatography with deae-sephadex a-25, hydroxyapatite, mono q, superose 12 and phenyl-superose. the mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. the isoelectric point of vm-rtdh was approximately ph 4.9. the hemolytic activity of vm-rtdh was stable upon heating at 100 degrees c for 10 min, similar to that ...19911804756
acute intoxication as a cause of wild bird mortality in the netherlands from 1975-1989. 20071805755
two generalized transducing phages in vibrio parahaemolyticus and vibrio alginolyticus.two bacteriophages named phi vp253 and phi vp143 isolated after ultraviolet induction from lysogenic strains of vibrio parahaemolyticus have been shown to be generalized transducing phages. so far, seven different auxotrophic markers of a v. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2 x 10(-7) to 7.5 x 10(-5) per infected cell at the m.o.i. of approximately 1.0. the phage phi vp143, but not phi vp253, lysed 20 of the 28 strains of v. alginolyticus and the occu ...19911808461
[identification of species of microorganisms of the genus vibrio].during 1988, a study was made on 61 microorganisms, genus vibrio, which were received at the national reference laboratory for acute diarrheic diseases. pedro kouri institute of tropical medicine. of them, 46 were from children with acute diarrheic disease and 15 were isolated from the environment. by means of biochemical tests. 61 vibrio cholerae no. 01. 9 vibrio parahaemolyticus and 1 vibrio alginolyticus were identified. emphasis is placed upon the importance of keeping a systematic surveilla ...19911812527
[a study on diarrhoea disease caused by vibrionaceae along coast the east of zhejiang province].a study of the etiology of 1028 cases of acute diarrhoea due to vibrionaceae was carried out between august 1988 to july 1990 along the coast of east zhejiang province. from 417 clinical cases (40.56%) were found. 590 strains (57.39%) of pathogenic vibrios among them, the detection rates of vibrio, aeromonas and plesionmonas were 48.14%, 44.74%, 7.12% respectively. there were 8 species of vibrio detected: v. parahemolyticus (63.73%), v. fluvialis (11.97%), v. alginolyticus (10.215), non-01 vibri ...19911816992
the use of potassium-titanyl-phosphate laser for laparoscopic removal of ovarian endometrioma.from january 1989 through january 1990, 31 patients thought to have ovarian endometrioma at ultrasonography were treated by operative laparoscopy and potassium-titanyl-phosphate laser. the endometriomas ranged from 2 to 9.2 cm in diameter by preoperative transvaginal ultrasonography. the operative procedure used was a three-puncture technique with a 600 micron fiber attached to a potassium-titanyl-phosphate laser. the power setting was 10 w with a continuous pulse. in all cases endometriomas wer ...20061828651
the use of potassium-titanyl-phosphate laser for laparoscopic removal of ovarian endometrioma.from january 1989 through january 1990, 31 patients thought to have ovarian endometrioma at ultrasonography were treated by operative laparoscopy and potassium-titanyl-phosphate laser. the endometriomas ranged from 2 to 9.2 cm in diameter by preoperative transvaginal ultrasonography. the operative procedure used was a three-puncture technique with a 600 micron fiber attached to a potassium-titanyl-phosphate laser. the power setting was 10 w with a continuous pulse. in all cases endometriomas wer ...20061828651
issues with radiation therapy article. 20061828682
issues with radiation therapy article. 20061828682
[retrospective epidemiologic analysis of the occurrence of measles in slovakia].the authors give a detailed account of the reported measles morbidity in slovakia during the past 50 years (almost half a million notified cases). before vaccination on a mass scale was introduced, some 1% of the notified cases died. the most frequent cause of death was pneumonia, followed by encephalitis. since 1977 there was not a single death from measles. before vaccination was introduced preschool children were affected most badly by the morbidity. after introduction of vaccination on a mas ...20071828717
structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6.we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ...19911828805
complexity and sequence identification of 24 rat v beta genes.twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ...19911828824
aggressive chemotherapy for acute leukaemia frequently causes intestinal protein leakage.cytostatic drugs are known to produce disturbances in intestinal absorption of carbohydrates. to further explore the gastrointestinal (gi) toxicity of cytostatic therapy, 37 patients with acute leukemia were investigated during and/or after remission induction courses by the use of the differential sugar absorption test (dsat) and the intestinal clearance of alpha-1-antitrypsin (claat). the ratio of the lactulose to the mannitol excretion in the urine was found abnormal in 44% of the tests. the ...20061828960
aggressive chemotherapy for acute leukaemia frequently causes intestinal protein leakage.cytostatic drugs are known to produce disturbances in intestinal absorption of carbohydrates. to further explore the gastrointestinal (gi) toxicity of cytostatic therapy, 37 patients with acute leukemia were investigated during and/or after remission induction courses by the use of the differential sugar absorption test (dsat) and the intestinal clearance of alpha-1-antitrypsin (claat). the ratio of the lactulose to the mannitol excretion in the urine was found abnormal in 44% of the tests. the ...20061828960
[mean values of risk factors for ischemic heart disease and their percentile distributions in the alma-ata population of men aged 20-54].the authors analyze the results of a one-stage epidemiological study of the open population of men aged 20-54 years (n-2886), carried out with a purpose of revealing coronary heart disease (chd) and its risk factors (rf). the study was performed with the aid of the standard who methods of epidemiological investigations. the prevalence of the main chd rf (arterial and borderline hypertension, overweight, tobacco-smoking, dyslipoproteinemias) was established. the mean values of rf in different age ...19911829274
survivals of vibrio parahaemolyticus and escherichia coli in a gastropod mollusc, heminerita japonica.vibrio parahaemolyticus strains d-3 and r-13 were found to be cleared within 7 days from a marine neritid gastropod mollusc, heminerita japonica, maintained in artificial seawater with salinities of 15, 25 and 35 permil (%) at 25 degrees c. escherichia coli strain ys-2 survived at a level of 10(2) colony forming units per gram in the mollusc maintained in 15% water for up to 14 days and fell to non-detectable level within 7 days in a 35% salinity group. the ability of h. japonica to clear these ...19911830783
attachment of vibrio parahaemolyticus strains to estuarine algae.attachment of vibrio parahaemolyticus strains to estuarine microalgae was examined in artificial seawater by viable counts of the organism and direct counts of the bacterial cells after immunoperoxidase staining. thermostable direct hemolysin (tdh)-producing and tdh-non-producing strains of v. parahaemolyticus were found to attach to five estuarine strains of navicula (diatom alga) in similar levels. the level of the bacterial attachment depended on salinity and temperature of the water, in whic ...19911830790
ecological cycle of thermostable direct hemolysin-producing strains of vibrio parahaemolyticus in a brackish-water area with special reference to molluscs and attached microalgae.prevalences of thermostable direct hemolysin (tdh)-producing strains in communities of a gastropod mollusc. clithon retropictus, and a bivalve mollusc, corbicula japonica, and levels of the strains in attached microalgae and muddy sediments were investigated at a brackish-water area along hashizu creek and togo pond in japan, v. parahaemolyticus was detected from attached microalgae at hashizu creek in summer months with the highest level of 1.4 x 10(5) cfu/g. levels of the organism among 20 ani ...19911830798
in vitro attachment of vibrio parahaemolyticus to hemocytes of two gastropod molluscs.vibrio parahaemolyticus d-3 was observed to attach to hemocytes of a marine gastropod mollusc, nerita albicilla, regardless of the presence of n. albicilla serum. the organism attached to hemocytes of an estuarine gastropod, clithon retropictus, in the presence of c. retropictus serum while the attachment to the hemocytes was decreased significantly in the absence of the serum. these evidences suggest that n. albicilla hemocytes would facilitate the clearance of v. parahaemolyticus from the alim ...19911830802
na(+)-coupled atp synthesis in a mutant of vibrio parahaemolyticus lacking h(+)-translocating atpase activity.an h(+)-translocating atpase-defective mutant of vibrio parahaemolyticus ys-1 grew well on lactate as a sole source of carbon at ph 8.5 under aerobic conditions, but not under anaerobic conditions. both wild type cells and the mutant cells could grow on lactate at ph 8.5 even in the presence of an h+ conductor, carbonylcyanide m-chlorophenylhydrazone (cccp), but not at ph 7.5. oxidative phosphorylation resistant to cccp in the mutant occurred at ph 8.5. these findings suggest the existence of na ...19911831976
plasma-dependent chemotactic activity of hemocytes derived from a juvenile estuarine gastropod mollusc, clithon retropictus, to vibrio parahaemolyticus and escherichia coli strains.hemocytes of adult clithon retropictus were attracted chemotactically to live vibrio parahaemolyticus and escherichia coli strains. the chemotaxis was stimulated by the plasma of adult c. retropictus. hemocytes of the juvenile specimen were attracted chemotactically to v. parahaemolyticus and e. coli strains in the presence of the plasma of the juvenile and only to e. coli strain in the absence of the plasma. these evidences suggest that hemocytes of juvenile c. retropictus might be defective to ...19911836376
phosphate-dependent anti-vibrio parahaemolyticus activity in neritid gastropod molluscs. 19911836388
[human gastroenteritis associated with vibrio parahaemolyticus in recife, brazil].a study was carried out on the occurrence of vibrio parahaemolyticus in 1,100 diarrheal feces, routinely sent to a private clinical laboratory for microbiologic diagnosis, in recife. v. parahaemolyticus was isolated from 14 (1.3%) fecal samples. however, if we considered only the specimens from adult patients, the isolation rate of v. parahaemolyticus rose to 7.1%. in most cases (92.86%), v. parahaemolyticus was the only enteropathogen recognized. among the isolates, seven k antigen serovars wer ...19911843400
isolation of urease-positive vibrio parahaemolyticus from diarrheal patients in northeast brazil.of 21 human fecal strains of vibrio parahaemolyticus, isolated on the northeast coast of brazil, eight (38%) were urease positive. most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the kanagawa phenomenon.19911844947
growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes.protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ...19911847373
regulation of alcohol consumption by the renin-angiotensin system: a review of recent findings and a possible mechanism of action.the renin-angiotensin system has traditionally been associated with the regulation of fluid and electrolyte balance. in this review we summarize the data which ascribes a completely new function to this system, i.e., the regulation of alcohol consumption. in addition, we suggest a possible mechanism for this effect based on the concept of a satiety or stop process. the approach taken was to examine the effect on alcohol intake of a wide variety of drug, genetic, dietary, surgical and neurosurgic ...20071852316
response by dr. alden n. haffner upon receipt of the koch memorial medal 1990. 20071852404
development of circadian sleep-wake rhythms in preterm and full-term infants.we have compared the roles of neurologic maturity and environmental time cues in the development of the entrained circadian sleep-wake rhythm in the preterm and term human infant. the preterm infants (n = 19) spent some time after birth in a hospital nursery with no environmental time cues, whereas the term infants (n = 22) were exposed from birth to a cyclical light and dark environment with one major caregiver. the circadian sleep-wake rhythm in the preterm infants entrained after a similar ti ...19911852533
[opinions and attitudes toward dentistry in early adolescence]. 20071852655
interaction of butorphanol, with monoamine oxidase inhibitor, tranylcypromine.this investigation examines the possibility of interaction between tranylcypromine and butorphanol in comparison to pethidine. the ld50 of pethidine and butorphanol were determined in mice pretreated with the non-selective monoamine oxidase (mao) inhibitor, tranylcypromine orally for 8 days or with oral saline solution. tranylcypromine decreased the ld50 of both pethidine and butorphanol by 78% and 41%, respectively. anesthetized rabbits with halothane pretreated with tranylcypromine or saline w ...19911855717
interaction of butorphanol, with monoamine oxidase inhibitor, tranylcypromine.this investigation examines the possibility of interaction between tranylcypromine and butorphanol in comparison to pethidine. the ld50 of pethidine and butorphanol were determined in mice pretreated with the non-selective monoamine oxidase (mao) inhibitor, tranylcypromine orally for 8 days or with oral saline solution. tranylcypromine decreased the ld50 of both pethidine and butorphanol by 78% and 41%, respectively. anesthetized rabbits with halothane pretreated with tranylcypromine or saline w ...19911855717
[laser coagulation of the cornea with a holmium:yag laser for correction of hyperopia].we present a new technique of hyperopic correction similar to radial thermokeratoplasty. a pulsed ho: yag laser was used that emitted radiation at 2.06 microns and was guided by a quartz fiber and a focusing hand piece. the penetration depth of this light in the cornea is about 400 microns. eight or 16 concentric point coagulations result in steepening in the central cornea. this refractive change is inverse related to the distance to the cornea center, and it increases linearly with increasing ...20071855726
[laser coagulation of the cornea with a holmium:yag laser for correction of hyperopia].we present a new technique of hyperopic correction similar to radial thermokeratoplasty. a pulsed ho: yag laser was used that emitted radiation at 2.06 microns and was guided by a quartz fiber and a focusing hand piece. the penetration depth of this light in the cornea is about 400 microns. eight or 16 concentric point coagulations result in steepening in the central cornea. this refractive change is inverse related to the distance to the cornea center, and it increases linearly with increasing ...20071855726
[lipid metabolism disorders: diagnosis and treatment. 4: drug therapy and dietary recommendations. national cholesterin-initiative]. 20061855762
[lipid metabolism disorders: diagnosis and treatment. 4: drug therapy and dietary recommendations. national cholesterin-initiative]. 20061855762
in vitro effect of phyllanthus amarus on hepatitis b virus.to evaluate the effects of p. amarus on hepatitis b virus (hbv) antigens and hbv-dna, initial ethanolic extract and subsequent fractions of the plants were prepared. the whole plant material was dried, powdered and extracted with alcohol and subsequently fractionated in hexane, chloroform, butanol and finally in water. all the material were tested for in vitro effects on hbsag, hbeag and hbv-dna in serum samples positive for hbv antigens followed by the screening of respective antigens by elisa. ...19911855821
relevance of n-nitrosamines to oesophageal cancer in china.oesophageal cancer occurs at a very high frequency in certain areas of china, especially in linxian county, henan province. previous studies suggested that n-nitroso compounds play a causative role. in order to study further the exposure of linxian inhabitants, the intake of n-nitrosamines in the diet was determined. the total daily intake of volatile nitrosamines and of n-nitroso-n-methylbenzylamine (nmbza) was higher in linxian than in two other counties. nmbza can induce cancer in animal and ...19911855832
similarity of the tdh gene-bearing plasmids of vibrio cholerae non-o1 and vibrio parahaemolyticus.the gene encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus was previously cloned from a plasmid of vibrio cholerae non-o1. the gene (designated as nag-tdh) was subcloned and its nucleotide sequence was determined and compared with reported sequences of the four tdh gene copies encoding tdh, of which three were cloned from the chromosome and one was cloned from a plasmid of v. parahaemolyticus. in the coding region, the nag-tdh gene had 100% homolo ...19911857199
how we teach ... practical skills in an undergraduate medical curriculum: 5 years of experience at gezira medical school (sudan).an outline of an undergraduate course to teach medical students practical medical procedures early in their curriculum is described. introduction of those skill as early as the second year has given the student ample time to practice those skills before graduation. feedback has shown that the course has been successful in helping the students to master simple procedures by the time they graduate. early demonstration of complicated skills has also facilitated the ability and confidence of the stu ...20071865801
animal rights. 19911865899
utility of leopold maneuvers in screening for malpresentation.malpresentation is a common complication of pregnancy. using ultrasound as the gold standard, we prospectively tested leopold maneuvers as a screen for nonvertex presentation in the third trimester. in our hands this technique performed poorly. values for sensitivity, specificity, positive predictive value, and negative predictive value were 28, 94, 24, and 95%, respectively, in a population with a 7% prevalence of noncephalic presentation. much of the correlation between the screening and diagn ...20071876372
distinction of mesothelioma from carcinoma in pleural effusions. an immunocytochemical study on routinely processed cytoblock preparations.the study was designed to find out whether the commercially available antibodies bma 130 c, bma 120, v 9, kl 1, b 72.3 tag, hea 125 and ber-ep 4 would be of help in distinguishing carcinomas from a mesothelial process (mesothelioma/pleuritis) in pleural effusion specimens routinely processed by the cytoblock method. all of the 20 carcinomas included in the study but also 19 of the 20 mesotheliomas expressed cytokeratin (kl 1), whereas vimentin expression was found in 7 of the 20 carcinomas and 1 ...20071876528
an evaluation of three commercial fecal transport systems for the recovery of enteric pathogens.twenty-five isolates, including six strains of shigella species, six strains of salmonella species, five strains of yersinia enterocolitica, six strains of campylobacter jejuni, and two strains of vibrio parahaemolyticus, were inoculated at a concentration of 1.5 x 10(4) colony-forming units/ml into the following transport systems: fekal enteric plus (trend scientific, inc., st. paul, mn), cary blair transport medium (remel, inc., lenexa, ks), and para-pak c & s (meridian diagnostics, inc., cinc ...19911877533
analysis of the gene of vibrio hollisae encoding the hemolysin similar to the thermostable direct hemolysin of vibrio parahaemolyticus.the gene (designated as vh-tdh) of vibrio hollisae 9041 encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of v. parahaemolyticus contained a 567-base-pair open reading frame (orf), which was 93.3-93.5% homologous to those of the tdh genes of v. parahaemolyticus, v. cholerae non-01, and v. mimicus encoding tdh or similar hemolysins. comparative analysis of the nucleotide sequence containing the vh-tdh orf with published nucleotide and amino acid sequences suggested that the ...19911884983
functional expression of furin demonstrating its intracellular localization and endoprotease activity for processing of proalbumin and complement pro-c3.we have cloned a rat cdna encoding furin which is structurally related to yeast kex2 protease. products of 88 and 94 kda were obtained by in vitro transcription/translation of the cdna in the absence and presence of microsomes. when the cdna was transfected into cos-1 cells, furin was expressed as a major glycosylated form of 94 kda, accompanied by a minor proteolytic form of 86 kda, and found to be localized in the golgi complex. proalbumin and complement pro-c3 are intracellularly processed in ...19911885622
discordant expression of tuberous sclerosis in monozygotic twins.a set of 20-year-old female japanese twins, most probably monozygotic, had clinical evidence of tuberous sclerosis (ts). they were discordant for symptoms. one of the twins exhibited facial red-brown papules (adenoma sebaceum), a dorsal shagreen patch, intracerebral calcifications, angiomyolipoma in the right kidney, and hypopigmented macules; the other had only a few hypopigmented macules. modification of ts gene expression by the effects of environmental (extrinsic) factors is suggested.20071885853
effects of environmental exposure on carbon polysulphone composites.in vivo and in vitro studies were designed to characterize the material degradation associated with implantation of carbon fibre-reinforced polysulphone (c/ps). composite plates were compression moulded for both studies, and a fibre orientation of 0 degrees with the long axis and a fibre volume fraction of approximately 55% were used. the in vitro experiment involved soaking three groups of four plates each at 4, 12 and 26 wk in 0.9% saline solution at 37 degrees c. the plates were measured for ...19911888812
characterization of thermostable direct hemolysins encoded by four representative tdh genes of vibrio parahaemolyticus.four tdh genes encoding thermostable direct hemolysin (tdh) cloned from two representative strains of v. parahaemolyticus (tdh 1 and tdh 2 from a hemolytic strain, tdh3 and tdh4 from a non-hemolytic strain) have different nucleotide sequences (the maximum divergence: 3.3%). in this study, each tdh gene product was purified from the lysate of escherichia coli cells carrying the cloned gene and their properties were compared to investigate the influence of the amino acid substitutions caused by th ...19911890953
intracerebral hemorrhage, cerebral infarction, and subdural hematoma after acute myocardial infarction and thrombolytic therapy in the thrombolysis in myocardial infarction study. thrombolysis in myocardial infarction, phase ii, pilot and clinical trial.in the thrombolysis in myocardial infarction, phase ii pilot and clinical trial, 908 patients [326 (35.9%) in the pilot study and 582 (64.0%) in the randomized study] were treated with 150 mg recombinant tissue-type plasminogen (rt-pa) activator in combination with heparin and aspirin, and 3,016 patients [64 (2.1%) in the pilot study and 2,952 (97.9%) in the randomized study] were treated with 100 mg rt-pa in combination with heparin and aspirin. adverse neurological events occurred in 23 patien ...20071899364
comparison of the indirect immunobead, radiolabeled, and immunofluorescence assays for immunoglobulin g serum antibodies to human sperm.the relative sensitivities of the indirect immunobead test, the indirect flo cytometric immunofluorescence assay, and an indirect radiolabeled antiglobulin assay were compared. eighteen immunobead test positive sera and 18 negative sera were used as the standard for the other two assays. of the 18 positive sera, 14 (77%) and 5 (27%) were positive in the immunofluorescence assay and the radiolabeled antiglobulin assay, respectively. four (22%) of the low titer immunobead test positive sera were n ...19911899399
comparative characterization of inducible and virulent vibrio parahaemolyticus bacteriophages having unique head projections.phage tp1, induced from vibrio parahaemolyticus k-20 pilot strain by mitomycin c, exhibited a unique hexagonal head with knob-like projections which covered the whole capsid and a noncontractile tail. the appearance of this phage was very similar to those of phages vp3 and vp6, isolated from seawater. the host range of phage tp1 was similar to those of phages vp3 and vp6. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the three phage particles revealed almost identical p ...19911908040
analysis of the tdh gene cloned from a tdh gene- and trh gene-positive strain of vibrio parahaemolyticus.a variant of the gene (tdh) encoding thermostable direct hemolysin (tdh) was cloned from the chromosome of vibrio parahaemolyticus aq3860, which gave positive results in the hybridization tests with the tdh gene probe and the trh (tdh-related hemolysin) gene probe and showed a low level of reaction in an enzyme-linked immunosorbent assay for tdh. nucleotide sequence analysis of the cloned gene (tdh5) provided no evidence that tdh5 is evolutionally closer to the trh gene than the other tdh genes. ...19911908042
the occurrence and growth of microorganisms during the fermentation of fish sausage.minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ...19911909546
cholera and vibrio parahaemolyticus diarrhoea endemicity in calabar, nigeria.the microbiological and morbidity profiles of acute diarrhoeal episodes were studied in 881 patients seen at the out-patients department of the university of calabar teaching hospital (ucth), calabar, between january and december, 1988. of a total of 108 (12.3%) culturally confirmed bacterial diarrhoeas, 47 (43.5%) were due to escherichia coli, 33 (30.6%) to vibrios (vibrio cholerae-01; classical and e1 tor biotypes and v. parahaemolyticus), while shigella spp. and salmonella. spp. accounted for ...19911911487
a novel mechanism of cation/substrate cotransport: na+/h+/adenosine cotransport in vibrio parahaemolyticus.adenosine is actively transported with na+ in vibrio parahaemolyticus (sakai, y., tsuda, m., tsuchiya, t. (1987) biochim, biophys. acta 893, 43-48). the proton conductor carbonylcyanide m-chlorophenylhydrazone, cccp, strongly inhibited active transport of adenosine at ph 8.5 as well as at ph 7.0. this seemed peculiar because the driving force, an electrochemical potential of na+, is established by the na(+)-extruding respiratory chain at ph 8.5 in this organism, although it is established by the ...19911911824
access to information for adult adoptees. 20071913424
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