Publications

lateral flagella of vibrios: serological classification and genetical similarity.	lateral (l-) flagella-having vibrios were classified into 13 h-serogroups (flagellar antigen serogroups) by means of h-agglutination test. vibrio parahaemolyticus was classified into 3 serogroups, hl1 to 3. v. alginolyticus and v. harveyi were classified into 5 and 3 serogroups, respectively, but 2 of those were serogroups common to the both species. v. fluvialis and v. furnissii constituted a same serogroup, hl8. cross-reactivity between each serogroup was not observed in h-agglutination test, ...	1992	1376395
ion channel selectivity, permeation, and block.		2006	1382215
disparate effects of calcium channel blockers on pressure dependence of renin secretion and flow in the isolated perfused rat kidney.	using the model of isolated perfused rat kidneys this study was performed to investigate whether or not voltage-operated calcium channels are essentially involved in the pressure control of renin secretion from the kidneys. at a perfusion pressure of 100 mm hg (13.3 kpa) renin secretory rates were 4.2 +/- 0.7 (ng angiotensin i h-1) min-1 g-1. stepwise reduction of renal perfusion pressure to 80, 60, and 40 mm hg (10.6, 8.0, 5.3 kpa) resulted in an increase of renin release yielding a 30-fold sti ...	1992	1382264
laparoscopic co2 laser uterine nerve ablation for treatment of drug resistant primary dysmenorrhea.	twenty women with drug-resistant primary dysmenorrhea were subjected to laparoscopic co2 laser uterine nerve ablation. menstrual pain assessed by a linear analog pain score showed a reduction of 33%, decreasing from 7.5 +/- 0.5 preoperatively to 5.0 +/- 1.7 postoperatively. the procedure was free of major complications and should be considered as a second-line therapeutic option in women who have failed medical treatment using nonsteroidal antiinflammatory agents or ocs.	2006	1386032
isolation and partial characterization of a compound with siderophore activity from vibrio parahaemolyticus.	a compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from dowex 1 x 8 extracts of culture supernatants of vibrio parahaemolyticus aq 3354. the strain synthesized the compound in culture media containing less than 2 microm added fecl3. hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. the 1h-nmr spectrum exhibited the presence of a residue from each of these components in the intact molecule. ...	1992	1387860
influence of dopaminergic and serotonergic neurons on intravenous ethanol self-administration in the rat.	rats implanted with chronic indwelling intravenous catheters and allowed access to a self-administration apparatus learned to self-inject intravenous ethanol. ethanol concentrations of 0.5, 1.0, and 2.0%, corresponding to a dose/injection of 1, 2, and 4 mg/kg, respectively, were consistently self-injected. self-injection was not acquired or maintained with ethanol doses of 0.5 or 8 mg/kg/injection. saline replacement of ethanol reservoirs led to marked increases in lever-pressing response in ani ...	1992	1388276
selective serotonin reuptake inhibitors.		2006	1392903
health checks on patients 75 years and over in nottinghamshire after the new gp contract.	to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.	2006	1393076
asthma and open cast mining.		2006	1393082
antimicrobial prophylaxis for fractured base of skull in children.	the details are reviewed of 50 children who were treated over a 10-year period with clinical signs of fractured base of skull. two patients died early without signs of sepsis--due to the severity of their head injuries. of the remainder, 23 received antibiotic prophylaxis and 25 did not. one patient from each of these groups developed pneumococcal meningitis, and they were successfully treated. our results correlate well with those previously published, confirming the low incidence of infective ...	2006	1393186
the measurement of radiant temperature in neonatal thermal environments.	heat exchange through radiation is recognized as the dominant mode of heat transfer for an infant nursed in an incubator or warmer. a radiometer was constructed to measure the planar radiant temperature experienced by the infant. two heat-flow sensors of different emissivities were mounted onto a heat sink such that one measured principally convective heat exchange while the other measured convective and radiant heat exchange. the radiant heat exchange was obtained from the difference between th ...	2006	1393209
current treatment of ulcerative colitis.	ulcerative colitis is a chronic relapsing disease which may become manifest either early or late in life. this article examines current drug treatment strategies and considers possible therapeutic options for the future.	2006	1393216
urease production in vibrio parahaemolyticus: a potential marker for virulence.		1992	1397235
incidence of toxigenic vibrios in foods available in taiwan.	a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ...	1992	1399913
vibrio-associated gastroenteritis in the lower cross-river basin of nigeria.	a total of 120 vibrio species were isolated from 588 patients with acute diarrheal disease during an outbreak of gastrointestinal tract infections at different locations in the lower cross river basin of nigeria. vibrio cholerae o1, biotype el tor, serotype ogawa, was the prominent organism isolated from the vibrio-associated diarrheal cases. during the 3 months of study, v. cholerae non-o1 was recovered from 10 patients while vibrio parahaemolyticus was recovered from 19 patients. the significa ...	1992	1400976
[in vitro antimicrobial activity of dr-3355, a new quinolone antibacterial agent, against clinical isolates of enteritis-causing bacteria].	we determined the minimum inhibitory concentration (mic) of dr-3355, a newly developed quinolone-derivative antibacterial agent, against clinical isolates of various bacterial species from enteritis patients, and compared them with those of ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), ampicillin (abpc), kanamycin (km). mic90 of dr-3355 against 94 strains of shigella spp. and 5 strains of escherichia coli, 36 strains of salmonella spp., 22 strains of vibrio cholerae, 5 strains of ...	1992	1402063
study of acute intraoperative suprachoroidal hemorrhage.	acute intraoperative suprachoroidal hemorrhage or effusion (aish) was studied in a prospective manner over the past three years. in a general cataract surgery population of 2,523 patient eyes, 15 (0.6%) experienced an aish. results of a specific surgical management technique are presented. surgical control of the aish consisted of immediate closure of the incision and application of pressure directly to the eye. this tamponades the effusion or hemorrhage, which allows coagulation and completion ...	2006	1403754
[methods to detect the thermostable direct hemolysin gene and a related hemolysin gene of vibrio parahaemolyticus by pcr].		1992	1404920
characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins.	irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ...	1992	1406279
chemotactic activity of hemocytes derived from a brackish-water clam, corbicula japonica, to vibrio parahaemolyticus and escherichia coli strains.	hemocytes from adult and juvenile specimens of a brackish-water clam, corbicula japonica, were attracted chemotactically to live cells of vibrio parahaemolyticus and escherichia coli strains in a balanced salt solution, which was enhanced significantly in the presence of respective c. japonica plasma. chemotactic attractions of adult's and juvenile's hemocytes were seen also in artificial seawater at a similar level to those in the balanced salt solution. chemotactic attractions of juvenile's he ...	1992	1420564
vibrio parahaemolyticus septicemia in a patient with neutropenic leukemia.		1992	1420695
the regulation of microsomal glucose-6-phosphatase differs between liver and kidney.		1992	1426558
participation of health workers, school teachers and pupils in the control of rheumatic fever: evaluation of a training programme.	in a rural community block of north india we initiated a programme for control of rheumatic fever and rheumatic heart disease (rf/rhd). this included a training campaign for all 74 health workers, 773 school teachers and 12,500 older pupils (class v to x) to enable them to suspect and refer cases of rf/rhd and counsel them about secondary prophylaxis. training material was used by project staff, medical officers and teachers to convey that this serious disease with onset between 5 and 15 years c ...	2006	1428137
psychological effects during reduced training volume and intensity in distance runners.	improved mood state has ben linked with reduced training (rt). however, rt has been measured only as a reduction in training volume, either maintaining or not considering the intensity of training employed. to investigate the effects of a 4-week reduction in both training volume and intensity on running performance and mood state, 10 well-trained adult male runners trained for 4 weeks at baseline training distance and pace (bt), followed by 4 weeks training reduced in volume by 66%, with intensi ...	2006	1428383
activation of t cells in vivo using anti-cd3 and staphylococcal enterotoxins.	one of the major problems in clinical immunity is that neoplastic and virally infected cells are insufficiently immunogenic to trigger an immune response. during the past several years, our laboratory has explored the use of t-cell-specific reagents including monoclonal antibodies (mabs) and staphylococcal enterotoxins to amplify immune responses. this report summarizes our efforts to augment immunity with these agents.	1992	1428401
thermostable, salt tolerant, wide ph range novel chitobiase from vibrio parahemolyticus: isolation, characterization, molecular cloning, and expression.	a chitobiase gene from vibrio parahemolyticus was cloned into plasmid puc18 in escherichia coli strain dh5 alpha. the plasmid construct, pc120, contained a 6.4 kb vibrio dna insert. the recombinant gene expressed chitobiase [ec 3.2.1.30] activity similar to that found in the native vibrio. the enzyme was purified by ion exchange, hydroxylapatite and gel permeation chromatographies, and exhibited an apparent molecular weight of 80 kda on sds-polyacrylamide gel electrophoresis. chitobiose and 6 mo ...	1992	1429506
modification of the disc assay by the incorporation of monoclonal antibody staining.	the use of immunohistochemical staining with the disc assay is described. this modified staining procedure allows differentiation of viable human tumor cells from normal cells in biopsy preparations, facilitating the evaluation of slides from chemosensitivity assays. samples from all and cll patients were processed using both the disc and modified moab-disc procedure. the predictive results were compared and found to be identical. this procedure is advantageous in determining the chemosensitivit ...	1992	1434746
use of an oligonucleotide probe to detect vibrio parahaemolyticus in artificially contaminated oysters.	a 26-mer oligonucleotide specific to vibrio parahaemolyticus was synthesized from a 1,275-bp thermostable direct hemolysin (tdh) gene. this oligonucleotide probe specifically reacted with dna from 89 of 95 v. parahaemolyticus isolates but not with dna from other vibrios or other enteric and nonenteric organisms (n = 48). the probe hybridized with southern blots of 0.5-kb hindiii-restricted chromosomal dna fragments from all but five v. parahaemolyticus test isolates. the probe could be used to d ...	1992	1444377
"raynaud's disease" in migraineurs: one entity or two?	two cases are reported of patients who were diagnosed as having both migraine and raynaud's disease. because they were able to achieve effective control of both headaches and extremity temperature after undergoing a comprehensive approach--utilizing medication, biofeedback, and psychotherapy--it is suggested that both conditions are manifestations of the same disorder. it is recommended that a first step in approaching concurrent diagnoses of this type involve combined treatment as is often used ...	2006	1446993
the european federation of cytology societies, 1969-1989.		2006	1449022
diaphragmatic movement using ultrasound during spontaneous and mechanical ventilation: effect of tidal volume.	using ultrasound (us) the effect of various tidal volumes on the movement of ventral, dome and dorsal parts of the right hemidiaphragm was studied, both during spontaneous and mechanical ventilation. six healthy non-medicated volunteers who were in the supine position breathed spontaneously shallowly (tidal volume (vt) being 400 ml) (sb), and deeply (vt 1000 ml) (sb-deep). in addition, they were mechanically ventilated with intermittent positive pressure ventilation at three different vt's: 500 ...	2006	1449048
[the comparative characteristics of vibrio cholerae hemolysins].	biochemical and biological properties of in vivo and in vitro hemolysins of cholera germs isolated by different authors as well as hemolysins of vibrio parahaemolyticus and escherichia coli have been comparatively characterized. according to the above data hemolysin of cholera germ el tor is a thermolabile protein with molecular mass of 60-80 thou.; it is cytotoxic, enteropathogenic and lyses all the species of erythrocytes. hemolysins of v. eltor and germs are immunologically related. hemolysin ...	1992	1453988
[changes in auriculoventricular conduction due to the effect of ethanol].	transcoronary ablation of atrioventricular conduction by dehydrated alcohol was attempted in one patient with refractory ectopic atrial tachycardia. ethanol (1.5 ml) was delivered after selective catheterization of the atrioventricular nodal artery in a patient in whom the artery could be identified by cineangiography. the mean creatine kinase (mb fraction) at four hour to six hour after ablation was 40 u. no electrocardiographic qrs changes was seen. the procedure was successful. the patient is ...	2006	1457004
haemorheology and exercise: intrinsic flow properties of blood in marathon running.	haemorheological and haematological parameters were studied in venous blood samples taken from 8 endurance-trained athletes before and after a marathon run. viscometry was performed in a 20 microns glass capillary and in a couette viscometer. apparent blood viscosity was lower in the capillary than in the rotational viscometer, but significant differences between pre- and post-run samples were not observed in either system. this is presumably due to the absence of changes of haematocrit (pre-run ...	2006	1459744
intravenous nifedipine for prevention of myocardial ischaemia after coronary revascularization.	we sought to determine the pharmacokinetic and pharmacodynamic behaviour of a continuous infusion of nifedipine given for prevention of myocardial ischaemia following coronary artery bypass graft (cabg) surgery. patients scheduled for elective cabg, who had good left ventricular function, were included. only normotensive patients who did not require treatment with vasoactive drugs and were bleeding less than 100 ml.hr-1 following surgery were included. the patients were randomly distributed into ...	2006	1464126
identification of amino acid changes affecting yeast uroporphyrinogen decarboxylase activity by sequence analysis of hem12 mutant alleles.	the molecular basis of the uroporphyrinogen decarboxylase defect in eleven yeast 'uroporphyric' mutants was investigated. uroporphyrinogen decarboxylase, an enzyme of the haem-biosynthetic pathway, catalyses the decarboxylation of uroporphyrinogen to coproporphyrinogen and is encoded by the hem12 gene in the yeast saccharomyces cerevisiae. the mutations were identified by sequencing the mutant hem12 alleles amplified in vitro from genomic dna extracted from the mutant strains. four mutations lea ...	1992	1471989
transforming growth factor beta and basic fibroblast growth factor synergistically promote early bovine embryo development during the fourth cell cycle.	developmentally competent bovine blastocysts were produced by adding transforming growth factor beta (tgf beta) and basic fibroblast growth factor (bfgf) to serum-free cultures of in vitro produced, 2-cell bovine embryos. the effects of tgf beta were evaluated because this growth factor signals synthesis and secretion of the extracellular matrix component fibronectin and its receptor. previous investigations have demonstrated that fibronectin promotes early bovine embryo development in vitro. th ...	1992	1472373
purification and characterization of kanagawa haemolysin from vibrio parahaemolyticus.	the haemolysin of a kanagawa-phenomenon-positive vibrio parahaemolyticus strain was purified to apparent homogeneity by acid precipitation, deae-trisacryl, hydroxyapatite and fplc (mono-q) columns: 1.4 micrograms of protein gave a single band on conventional sds-page with silver staining. the haemolysin was not inactivated by heating for 10 min at 100 degrees c. it was a monomeric protein with a molecular weight estimated to be 29 kda by page under denaturing and non-denaturing conditions. the h ...	1992	1475518
the thermostable direct hemolysin of vibrio parahaemolyticus is a pore-forming toxin.	the hemolytic mechanism of thermostable direct hemolysin (tdh), a possible virulence factor of vibrio parahaemolyticus, was studied. we demonstrated that tdh acts as a "pore-forming toxin" in temperature-dependent and -independent steps. the first temperature-dependent step requires only about 1-2 min incubation at 37 degrees c and makes a "pore" with a functional diameter of approximately 2 nm. the pore size was deduced from the molecular diameter of the colloidal inhibitory polysaccharides. th ...	1992	1477791
angiokeratoma of the clitoris: a subtype of angiokeratoma vulvae.	a 24-year-old married woman had an angiokeratoma of the vulva measuring 3 x 3 x 4 mm localized at the clitoris. the nodule was noticed when she was pregnant with her first child. this tumor grew with occasional tenderness and bleeding during pregnancy, but its size did not change after delivery. the growth of this tumor might be attributable to an increase in venous pressure or serum progesterone levels; therefore this disorder might be classified as a subtype of angiokeratoma of the vulva. this ...	2006	1479114
ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride.	intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ...	1992	1479187
detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus by polymerase chain reaction.	polymerase chain reaction (pcr) protocols were established for specific detection of the tdh and trh genes, the virulence marker genes of vibrio parahaemolyticus encoding two related hemolysins. the tdh and trh genes are known to have sequence divergence of up to 3.3% and 16%, respectively. attempts were made to find suitable primer pairs and annealing temperatures to detect each gene without fail. dnas extracted from 36 representative strains of v. parahaemolyticus were used in the initial scre ...	1992	1480187
non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification: application to detection of the tdh gene of vibrio parahaemolyticus.	a non-isotopic microtitre plate-based assay method was devised for detection of products of the polymerase chain reaction. this assay involves affinity immobilization of the biotinylated amplification products in microtitre plate wells and their fluorescence detection by their hybridization with an oligonucleotide probe linked to alkaline phosphatase. an advantage of this procedure is that the immobilization and hybridization are carried out simultaneously in the wells, thus shortening the assay ...	1992	1480188
[the phages of halophilic vibrios and their use].	the range of the lytic activity of 46 phages of parahemolytic vibrios isolated from lysogenic strains, sea water samples, crabs and mussels has been studied. the phages are represented by virions belonging to morphological groups ii, iv, v according to the phage classification currently used in russia and to different serological groups. no relationship between the sensitivity of vibrio strains to the phages under study and the specificity of serotypes o and k has been established. the preparati ...	1992	1481605
performance appraisal of online medline access routes.	to compare the performance and cost of 11 online medline systems with medline at elhill.	2006	1482922
a critical appraisal of the literature on the effects of computer-based clinical decision support systems on clinician performance and patient outcomes.	to review the evaluations of computer-based clinical decision support systems (cdss's).	2006	1482947
optimizing staff scheduling by monte-carlo simulation.	docs is a computer program which generates the staff schedule. an accounting framework is combined with an optimization technique that searches for a schedule in which all accounts are simultaneously in balance. the search is accomplished using a monte-carlo process which shuffles staff within the schedule. the shuffling is biased according to each staffer's account balance: the staffer who owes the most is most likely to be scheduled.	2006	1482957
professional doc (pdoc): a software environment for authoring and consulting of electronic documentation. interactive diagnosis and therapy: a medical application.	professional doc (pdoc) is a software environment for electronic documentation authoring and consulting based on the hypermedia technology. it allows to manage different kinds of information (pictures, sounds, texts, graphics, video) organized according to hyperstructures. the user-friendliness and effectiveness of its man-machine interface, and its availability on the most common hardware platforms makes it a suitable product for a very broad category of users. interactive diagnosis and therapy ...	1992	1483002
organ donation in intensive care--a look at the ethical issues.	this paper is based on consideration of the ethical issues surrounding organ donation. this emotive subject has far-reaching implications concerning both donation and transplantation but the purpose in this paper is to deal specifically with issues related to cadaveric organ donation and how they concern nurses in an intensive care unit (icu). a brief, general description of both ethics and organ donation is followed by a discussion of the issues surrounding the donor himself, including the diag ...	2006	1483023
radiotherapy of malignant melanoma of the uvea with i-125 seeds.	nineteen patients with malignant uveal melanomas were treated with i-125 applicators. there were 10 males and 9 females with a median age of 61 years (range 42-76). the tumour was located in the choroid in 12 eyes and in the choroid and ciliary body in 7 eyes. the size of the tumours was 7-18 mm in maximal basal diameter (median 12), 5-16 mm in minimal basal diameter (median 10), and 5.5-15 mm in thickness (median 8.5). the volume of the tumours was 123-1890 mm3 (median 540). all tumours were cl ...	2006	1488887
fatal outcome with use of rectal morphine for postoperative pain control in an infant.		2006	1489382
[nursing--a woman's job!].		2006	1489626
[the suicide phenomenon and fermentative metabolic activity in strains of the aeromonas group isolated from feces].	we study the "suicide" phenomena as well as metabolic pathways of mixed acids (methyl red test, mr) and butylene glycol (voges-proskauer, vp), in 107 strains belonging to mesophilic aeromonas group, isolated from stools. the strains have been identified as a. hydrophila, 28 cases (26.1%), a. sobria 26 cases (24.3%) and a. caviae 53 cases (49.6%). all a. caviae strains behave homogeneously as f+, rm+ and vp-, independently of temperature (30 or 37 degrees c). a. hydrophila strains only showed the ...	1992	1489794
susceptibility of the brine shrimp artemia and its pathogen vibrio parahaemolyticus to chlorine dioxide in contaminated sea-water.	adults and nauplii of the brine shrimp, artemia, together with vibrio parahaemolyticus, were placed in sewage-contaminated sea-water which had been treated with chlorine dioxide (hallox e-100tm) to test its potential as a disinfectant for salt water aquaculture. the nauplii were very susceptible to low concentrations of chlorine dioxide (47 micrograms/l cl-), but the adults were slightly more resistant. sterile sea-water treated with lower concentrations of chlorine dioxide (less than 47 microgr ...	1992	1490907
enterotoxigenicity of vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin.	vibrio parahaemolyticus produces a thermostable direct hemolysin (tdh) that has been implicated in the pathogenesis of diarrheal disease caused by this organism. however, previous studies attempting to demonstrate the contribution of the hemolysin to virulence have been inconclusive. we investigated this putative virulence factor by using an isogenic tdh-negative (tdh-) strain constructed by specifically inactivating the two copies of the tdh gene encoding tdh. the enterotoxigenicities of the pa ...	1992	1500161
behavioral analysis of vibrio parahaemolyticus variants in high- and low-viscosity microenvironments by use of digital image processing.	digital image analysis and light microscopy were used to study and quantify the growth and behavior of two variants and selected flagellar mutants of vibrio parahaemolyticus in glass flow cells under high- and low-viscosity conditions. the observations showed a series of surface-associated behaviors, including attachment, microcolony formation, migration, chemotactic movements, and aggregation, indicating a substantial degree of adaptive flexibility and multicellular behavior during growth of v. ...	1992	1512206
sequence variation in the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus.	our previous molecular epidemiologic study with gene probes (h. shirai, h. ito, t. hirayama, y. nakamoto, n. nakabayashi, k. kumagai, y. takeda, and m. nishibuchi, infect. immun. 58:3568-3573, 1990) demonstrated that the gene (trh) encoding a thermostable direct hemolysin-related hemolysin was strongly associated with clinical strains of vibrio parahaemolyticus. strain-to-strain variation in the intensities of the hybridization signals observed in the above study also suggested that the trh gene ...	1992	1514791
development of the immunomagnetic enrichment method selective for vibrio parahaemolyticus serotype k and its application to food poisoning study.	a method using immunomagnetic separation was developed to isolate the specified k serotype of vibrio parahaemolyticus from a mixture of a large number of bacteria with other k serotypes. this method was applied to food poisoning studies and could recover the v. parahaemolyticus serotype found in the patient from the incriminated foods.	1992	1514817
[sensitivity of vibrio and aeromonas to antibiotics].	the antibiotic sensitivity of 696 cultures belonging to the family vibrionaceae (v. cholerae o1, v. cholerae non-o1, v. albensis, v. parahaemolyticus, v. alginolyticus and aeromonas spp.) was studied and general regularities of the antibiotic sensitivity were shown: a high sensitivity to broad-spectrum antibiotics (tetracycline and chloramphenicol) and a low sensitivity to ++beta lactams (carbenicillin and ampicillin). the comparative examinations revealed similarity in the antibioticograms of v ...	1992	1514858
evidence for the existence of a restriction-modification system common to several species of the family vibrionaceae.	a broad-host-range vibriophage kvp40 originally isolated on vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five vibrio and one photobacterium species. 1010 was a non-restricting host. an anti-restriction mutant kvp40 aar1 was isolated after propagating the phage on a restricting host, v. anguillarum vib36. kvp40 aar1 grown on either 1010 or vib36, as well as the parental phage grown on vib36, showed much higher efficiencies of plating on all the restricting hosts ...	1992	1521769
intraperitoneal immunotherapy for ovarian cancer with alpha interferon.		2006	1524888
histological analysis of forelimb regeneration in the california newt taricha granulosa.	the regenerative ability of the forelimbs of the california rough-skinned newt, taricha granulosa was determined and compared to the same ability of the adult mexican axolotl, ambystoma mexicanum. forelimbs were amputated distally at the wrist and limbs removed at 1, 2, 3, 4, 5 and 6 weeks post-amputation were examined by histological analysis. since vitamin a and its derivatives cause extreme changes in pattern formation in the regenerating amphibian limb, we decided to study the ability of ret ...	2005	1525332
histological analysis of forelimb regeneration in the california newt taricha granulosa.	the regenerative ability of the forelimbs of the california rough-skinned newt, taricha granulosa was determined and compared to the same ability of the adult mexican axolotl, ambystoma mexicanum. forelimbs were amputated distally at the wrist and limbs removed at 1, 2, 3, 4, 5 and 6 weeks post-amputation were examined by histological analysis. since vitamin a and its derivatives cause extreme changes in pattern formation in the regenerating amphibian limb, we decided to study the ability of ret ...	2005	1525332
effects of iron limitation on production of a siderophore, outer membrane proteins, and hemolysin and on hydrophobicity, cell adherence, and lethality for mice of vibrio parahaemolyticus.	vibrio parahaemolyticus is one of the most important enteropathogens in taiwan, japan, and other coastal regions. the pathogenesis of v. parahaemolyticus disease is not clearly understood. the expression of some factors by v. parahaemolyticus in iron-rich and iron-limited media was analyzed. in the clinical hemolytic strains, the production of a siderophore, two outer membrane proteins (77 and 80 kda), and thermostable direct hemolysin was significantly enhanced in iron-limited culture, and hemo ...	1992	1535342
better right than quick?		2006	1538635
risk factors for suicide attempts in a human immunodeficiency virus screening program.		1992	1539099
hepatitis update. the delta virus.		2006	1539959
treatment of alopecia areata with diphenylcyclopropenone.		2006	1552076
cloning and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to retinaldehyde-binding protein and yeast sec14p.	protein tyrosine phosphorylation is important in the regulation of cell growth, the cell cycle, and malignant transformation. we have cloned a cdna that encodes a cytosolic protein-tyrosine-phosphatase (ptpase), meg2, from meg-01 cell and human umbilical vein endothelial cell cdna libraries. the 4-kilobase cdna sequence of ptpase meg2 corresponds in length to the mrna transcript detected by northern blotting. the predicted open reading frame encodes a 68-kda protein composed of 593 amino acids a ...	1992	1557404
animal rights/liberation philosophy.		2006	1563603
[advances in fetal medicine].		2006	1563637
[laser therapy of external genital lesions].		2006	1563686
lifestyle adjustments in elderly patients after burn injury.	although survival of older burn victims has improved significantly in recent years, controversy remains over the care that should be given to this group and the functional recovery of survivors. to assess the impact of burn injury on lifestyle in the elderly, we conducted a mail-in survey among surviving patients ages 45 years or older who were treated in our burn center from 1978 to the middle of 1987. the survey asked questions about living arrangements, personal care, family relations, social ...	2001	1572857
[treatment of cryoglobulinemia].		2006	1578700
detection and quantitative assessment of a vibrio cholerae o1 species in several foods by a novel enzyme immunoassay.	a selected antibody enzyme immunoassay (saeia) for the general detection of vibrio cholerae o1 species has been developed using the immunological reagents of a rabbit antiserum specific for v. cholerae o1 classical inaba 569b and immobilized cell fragments of v. cholerae o1 el tor 85p6, and beta-d-galactosidase-labeled goat anti-rabbit immunoglobulin g as tracer. the saeia was specific for v. cholerae o1 species and showed low cross-reaction values to other microorganism species tested including ...	1992	1584068
a broad-host-range vibriophage, kvp40, isolated from sea water.	a broad-host-range vibriophage, kvp40, was isolated from sea water by using vibrio parahaemolyticus 1010 (eb101) as the indicator host. the host range of kvp40 extended over at least 8 vibrio and 1 photobacterium species. kvp40 was a large tailed phage containing double-stranded dna and belonged to ackermann's morphotype a2. kvp40 dna was cleaved by 11 different type ii restriction endonucleases including ecori and hindiii, but not by 17 other enzymes including bamhi, kpni and sali.	1992	1584076
neonatal nurse practitioners--a view from perfidious albion?		2006	1586193
the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes.	chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ...	1992	1594593
ventriculopleural shunting used as a temporary diversion.	due to the limited absorptive capacity of the pleural cavity, infants and young children are not generally ideal candidates for ventriculopleural shunts. we report using chest cavities as alternate for temporary diversion of csf in a young child. venous access to the cervical region could not be utilized because of scarring from previous procedures, while peritoneal access was contraindicated due to repeated pseudocyst formation. pleural effusions were removed by thoracentesis when necessary, an ...	2006	1595400
diary from a week in practice.		2006	1595508
common infections in the elderly.	symptoms of infection in the elderly may be absent, vague or atypical. infection should be suspected when an elderly patient presents with a decline in well-being or with non-specific symptoms such as falls, dizziness, confusion, anorexia or weakness. common infections include bacterial pneumonia, urinary tract infection, intra-abdominal infections, gram-negative bacteremia and infection of decubitus ulcers. antibiotic therapy is not recommended for asymptomatic bacteriuria or locally infected d ...	2006	1595517
electrical cardioversion after amiodarone administration.	the possible effect of amiodarone administration on the effectiveness and complications of electrical cardioversion of supraventricular tachyarrhythmias has not been properly assessed. to investigate the effects of amiodarone on cardioversion, we performed 130 electrical cardioversion procedures in 116 patients who were receiving long-term amiodarone therapy (group i) and 44 cardioversion procedures in 43 patients who were receiving intravenous infusions of amiodarone (group ii). all patients in ...	2006	1595532
haemagglutinating and buccal epithelial cell adherence activities of vibrio parahaemolyticus: correlation with virulence.	clinical and environmental isolates of vibrio parahaemolyticus were examined for their ability to agglutinate human and rabbit erythrocyte and to adhere to human buccal epithelial (hbe) cells in the presence or absence of mannose. all strains produced cell-associated haemagglutinins (hgs) after 3 h at 37 degrees c. mannose-sensitive haemagglutination (msha) appeared to be a significant marker for differentiating between clinical and environmental isolates; 75% of clinical and 11% of environmenta ...	1992	1597877
comparison of vibrio parahaemolyticus hemolysin (vp-trh) produced by environmental and clinical isolates.	tdh-related hemolysin (vp-trh) produced by kanagawa-phenomenon-negative (kp-) vibrio parahaemolyticus has been demonstrated to be a possible virulence determinant. though almost half of kp- isolates examined from diarrhoeal patients produced vp-trh, few reports mentioned the ability of environmental isolates to produce vp-trh. considering the route of infection with v. parahaemolyticus, this toxin must be produced by the organisms in the sea or in sea food. to confirm that vp-trh produced by v. ...	1992	1601286
chemotactic activity of hemocytes derived from two marine neritid gastropod molluscs, nerita albicilla and heminerita japonica, to vibrio parahaemolyticus and escherichia coli strains.	hemocytes of two marine neritid gastropods, nerita albicilla and heminerita japonica, were attracted chemotactically to live vibrio parahaemolyticus and escherichia coli strains. chemotactic attraction of n. albicilla hemocytes was enhanced in the presence of n. albicilla plasma, while that of h. japonica hemocytes was not enhanced in the presence of h. japonica plasma. chemotactic activity of the hemocytes seems to participate in the rapid elimination of v. parahaemolyticus from these gastropod ...	1992	1606253
how accurate is a computerized surgical audit when resident medical staff collect the data?	since july 1988 all eight general surgeons at fremantle hospital have used a computer-based surgical audit and discharge system. at the time of writing (september 1991) 10,919 computer-generated discharge letters have been produced by the system. this paper describes the system and reports a series of quality control assessments carried out between 1 july 1988 and 30 june 1990 during which 30 pre-registration surgical residents completed 5,716 data collection forms. it was found that: (1) data c ...	2006	1610325
examination by site-directed mutagenesis of the amino acid residues of the thermostable direct hemolysin of vibrio parahaemolyticus required for its hemolytic activity.	the amino acid sequences of eight variants of thermostable direct hemolysin (tdh) and a tdh-related hemolysin, deduced from nucleotide sequences, were compared. fourteen amino acid residues, mainly within conserved regions, were chosen to prepare mutant tdhs with one or two amino-acid replacements by site-directed mutagenesis. of the 25 mutant tdhs prepared, those with replacements of trp65 (trp65-his65, trp65-tyr65, trp65-leu65) or leu66 (leu66-ser66) showed very significant reduction (more tha ...	1992	1630298
low ph deforms the influenza virus envelope.	influenza virus membrane fusion is induced by low ph, which triggers an irreversible conformational change in the viral haemagglutinin (ha). the result of this change is the extrusion of the ha fusion peptide, after which it may act in the fusion of virus and endosomal membranes. here we describe electron microscopic observations on low ph-treated virus after negative staining or cryo-electron microscopy of virus in the frozen hydrated state. the results indicate a destabilization of the virus m ...	1992	1634881
[hairy cell leukemia successfully treated with 2'-deoxycoformycin following refractoriness to splenectomy and alpha-interferon therapy].	a 34-year-old man was admitted in may, 1989 because of severe left upper abdominal pain, which was caused by advanced splenomegaly. on initial examinations, peripheral blood showed leukocytosis (13,400/ml) including 60% hairy cell which also infiltrated in bone marrow (64%). the patient was diagnosed as having hairy cell leukemia (japanese type) of b-cell lineage. splenectomy was performed as an initial treatment. the effect of splenectomy was only palliative and transient leukocytosis progresse ...	1992	1635172
total hepatic mesh wrap for hemostasis.	to improve hemostasis and avoid the complications of perihepatic packing, a technique of hepatic tamponade using total mesh wrap is reported. the method is geometrically, technically and mechanically feasible and appears to be effective in controlling severe parenchymatous bleeding.	1992	1636144
serum autoantibodies in patients with alzheimer's disease and vascular dementia and in nondemented control subjects.	in this study we sought to evaluate the clinical significance of serum autoantibodies to dementing processes.	2006	1636181
[eye foreign bodies. patients should be sent to the eye clinic for treatment].		2006	1636298
enzyme-labeled oligonucleotide probes for detection of the genes for thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) of vibrio parahaemolyticus.	alkaline phosphatase conjugated oligonucleotide probes were developed to detect the genes (tdh and trh) coding for the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) of vibrio parahaemolyticus. using dot blot hybridization, probes were tested with 94 clinical isolates of v. parahaemolyticus. results agreed well with those obtained using radio-labeled recombinant dna probes for the genes tdh and trh. specificity and sensitivity of enzyme tdh probes for detection of the trh ge ...	1992	1643584
isolation and characterization of new restriction endonucleases from vibrio parahaemolyticus: vpak32i enzyme with the class-iis heptanucleotide specificity, gctcttcn1/n4.	six restriction endonucleases (enases), classified into four different specificities, were found in a screen among 68 reference strains of vibrio parahaemolyticus of human origin. five of these enases are isoschizomers of well-known enases, while the remaining one, designated vpak32i, is a novel and highly efficient class-iis enase with the hepatanucleotide recognition site, 5'-gctcttc(1/4)-3'.	1992	1644299
evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among vibrio species.	the tdh gene of vibrio parahaemolyticus which encodes the thermostable direct hemolysin has been found in some strains of other vibrio species. analysis of seven tdh genes cloned from v. parahaemolyticus, vibrio mimicus, and non-o1 vibrio cholerae revealed that all tdh genes were flanked by insertion sequence-like elements (collectively named isvs) or related sequences derived from genetic rearrangement of isvs. the isvs possessed 18-bp terminal inverted repeats highly homologous to those of is9 ...	1991	1650342
obligatory role of ca2+ in the cytotoxic activity of dengue virus-induced cytotoxin.	the role of calcium ions (ca2+) in the cytotoxic activity of the dengue type 2 virus (dv)-induced macrophage (m phi) cytotoxin (cf2) was investigated in the present study. the findings show that cf2 prepared in ca(2+)-free medium had no cytotoxic activity on normal mouse spleen cells suspended in ca(2+)-free medium but killed the cells suspended in the medium with ca2+. substitution with calcium chloride restored the cytotoxic activity of cf2 the optimal dose being 10(-4) m concentration. cf2 in ...	1991	1653589
chemical structure of the carbohydrate backbone of vibrio parahaemolyticus serotype 012 lipopolysaccharide.	the chemical structure of the saccharide portion of vibrio parahaemolyticus serotype 012 lipopolysaccharide was studied. using chemical degradation and modification, as well as methylation analysis in combination with glc-ms, laser-desorption mass spectrometry and 1h-nmr and 13c-nmr spectroscopy, the carbohydrate backbone of the lipopolysaccharide was characterized as a branched decasaccharide with the following structure: (formula; see text) in the native lipopolysaccharide two additional phosp ...	1991	1655425
[isolation of enteropathogenic microorganism from patients with infection of the digestive tract during 1976 to 1988 in tenri hospital].	enteropathogenic microorganisms isolated from feces of 9,393 patients with diarrhea or enteritis in our hospital between 1976 and 1988 were analyzed. as the result of the examination of 5,443 outpatients, 1,811 strains of pathogens were isolated from 1,686 cases (31.0%). several species including salmonella spp., escherichia coli serotype, vibrio parahaemolyticus, were isolated before 1978, and the incidence of pathogens was low (14.8%). for the 10-year period since 1979, the incidence markedly ...	1991	1655921
regulation of the mouse mammary tumor virus (mmtv) binding site in cultured mammary tissue.	the mouse mammary tumor virus (mmtv) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. the hormonal regulation of this protein was studied both in vitro and in vivo. in mammary gland explant culture, levels of the mmtv binding protein were maintained only when prolactin was present in the medium. the further addition of progesterone induced levels to 164% of controls, while estradiol was without effect. these results were reproduce ...	1991	1665450
purification and characterization of pili isolated from vibrio parahaemolyticus na2.	pili from vibrio parahaemolyticus na2 isolated from a patient with diarrhea were purified and characterized. the organisms were hemagglutinative, but the purified pili were not. na2 pili were physicochemically and immunologically quite different from the previously described v. parahaemolyticus ha7 pili. nevertheless, there was a high degree of homology between their n-terminal amino acid sequences.	1991	1670933