| pathogenic mechanisms of actin cross-linking toxins: peeling away the layers. | actin cross-linking toxins are produced by gram-negative bacteria from vibrio and aeromonas genera. the toxins were named actin cross-linking domains (acd), since the first and most of the subsequently discovered acds were found as effector domains in larger martx and vgrg toxins. among recognized human pathogens, acd is produced by vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. upon delivery to the cytoplasm of a host cell, acd covalently cross-links actin monomers into non-polym ... | 2017 | 27858184 |
| microbial herd protection mediated by antagonistic interaction in polymicrobial communities. | in the host and natural environments, microbes often exist in complex multispecies communities. the molecular mechanisms through which such communities develop and persist - despite significant antagonistic interactions between species - are not well understood. the type vi secretion system (t6ss) is a lethal weapon commonly employed by gram-negative bacteria to inhibit neighboring species through delivery of toxic effectors. it is well established that intra-species protection is conferred by i ... | 2016 | 27637882 |
| penicillium chrysogenum dsoa associated with marine sponge (tedania anhelans) exhibit antimycobacterial activity. | a strain of penicillium chrysogenum was isolated from tedania anhelans (marine sponge) collected from indian ocean (8°22'30″n latitude and 76°59'16″ longitude) and deposited in culture collection centers. the strain subjected to different culture conditions for production of extrolites were extracted using ethyl acetate and chloroform. when both extracts were subjected for antibacterial activity, latter had high activity. minimum inhibitory concentration of chloroform extract ranged from 31.25-1 ... | 2016 | 26717859 |
| actin-directed toxin. acd toxin-produced actin oligomers poison formin-controlled actin polymerization. | the actin cross-linking domain (acd) is an actin-specific toxin produced by several pathogens, including life-threatening spp. of vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. actin cross-linking by acd is thought to lead to slow cytoskeleton failure owing to a gradual sequestration of actin in the form of nonfunctional oligomers. here, we found that acd converted cytoplasmic actin into highly toxic oligomers that potently "poisoned" the ability of major actin assembly proteins, ... | 2015 | 26228148 |
| establishment and characterization of fin-derived cell line from ornamental carp, cyprinus carpio koi, for virus isolation in india. | cyprinus carpio koi fin (cckf) cell line was established and characterized from the caudal fin tissue of ornamental common carp, c. carpio koi. this cell line has been maintained in l-15 medium supplemented with 15% foetal bovine serum (fbs) and subcultured more than 52 times over a period of 24 mo. the cckf cell line consisted of epithelial cells and was able to grow at temperatures between 22 and 35°c with an optimum temperature of 28°c. the growth rate of these cells increased as the proporti ... | 2015 | 25990269 |
| a comparative study of bacterial and parasitic intestinal infections in india. | infectious diarrhea causes a major health problem in developing countries with significant morbidity and mortality. very often, rehydration therapy alone does not suffice, mandating the use of antimicrobial agents. however, rapidly decreasing antimicrobial susceptibility is complicating the matters. | 2015 | 25954615 |
| characterization and structure elucidation of antibacterial compound of streptomyces sp. ecr77 isolated from east coast of india. | forty marine actinobacteria were isolated from the sediments of east coast (bay of bengal) region of tamilnadu, india. morphologically distinct colonies were primarily tested against fish pathogenic bacteria such as vibrio cholerae, v. parahaemolyticus, v. alginolyticus, pseudomonas fluorescens and aeromonas hydrophila by cross-streak plate method. the secondary metabolites produced by the highly potential strain cultured on starch casein broth were extracted separately with various solvents suc ... | 2015 | 25656264 |
| quantitative and qualitative analyses of the bacterial microbiota of tilapia (oreochromis niloticus) cultured in earthen ponds in the philippines. | the quantity and composition of the bacterial microbiota in the rearing water, sediment, gills and intestines of tilapia oreochromis niloticus collected every 2 weeks from day 30 to day 120 after stocking for grow-out culture in 6 earthen brackish water ponds in the philippines were examined. the total heterotrophic aerobic bacterial counts obtained in the water, sediment, gills and intestines of tilapia ranged from 10(3) to 10(4) c.f.u. ml(-1), 10(3)-10(5), 10(5)-10(7) and 10(4)-10(7) c.f.u. g( ... | 2015 | 25555375 |
| thermodynamic properties of the effector domains of martx toxins suggest their unfolding for translocation across the host membrane. | martx (multifunctional autoprocessing repeats-in-toxin) family toxins are produced by vibrio cholerae, vibrio vulnificus, aeromonas hydrophila and other gram-negative bacteria. effector domains of martx toxins cross the cytoplasmic membrane of a host cell through a putative pore formed by the toxin's glycine-rich repeats. the structure of the pore is unknown and the translocation mechanism of the effector domains is poorly understood. we examined the thermodynamic stability of the effector domai ... | 2014 | 24724536 |
| establishment of caudal fin cell lines from tropical ornamental fishes puntius fasciatus and pristolepis fasciata endemic to the western ghats of india. | two new cell lines, pff and cff were established from the caudal fin of the puntius fasciatus, and pristolepis fasciata respectively. since their initiation, these cell lines (pff and cff) have been subcultured in l-15 medium with 10% fetal bovine serum for more than 35 passages at 28°c and both the cell lines were characterized. karyotyping analysis of pff and cff cells at 25th passage indicated that the modal chromosome number was 2n=50 and 2n=48 respectively. the cell line was cryopreserved i ... | 2013 | 23933168 |
| synthesis and concentration dependent antibacterial activities of cuo nanoflakes. | we report, synthesis and antibacterial activities of cuo nanoflakes. cuo nanoparticles are prepared at room temperature through sol-gel method. x-ray diffraction studies show the particles are monoclinic (crystalline) in nature. scanning electron microscopy (sem) images clearly show that the prepared particles are flake like in structure. fourier transform infrared (ftir) spectra exhibits three different bands that correspond to the au and bu modes. antibacterial studies were performed on shigel ... | 2013 | 23498227 |
| antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. | dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. in this context, the validations of the traditional claim that the leaf extract of d. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. the bacterial biofilms were quantified by crystal violet staining. among the 17 bacterial pathogens screened ... | 2012 | 23115018 |
| antimicrobial activity of bioactive compounds and leaf extracts in jatropha tanjorensis. | jatropha tanjorensis was investigated scientifically to generate evidence for the efficacies reported in traditional systems and the results are given here. | 2012 | 22884742 |
| detection of toxigenic vibrio cholerae with new multiplex pcr. | vibrio cholerae (v. cholerae) is the etiological agent of epidemic cholera. in recent years, cholera has become endemic in different regions of the world. traditional culture and microscopic methods are considered the standard for the diagnosis of v. cholerae. however, these methods require days for confirmation. therefore, molecular methods that may be used for the sensitive, accurate, and rapid analysis of v. cholerae are urgently needed. | 2012 | 22632601 |
| clinical and epidemiologic features of diarrheal disease due to aeromonas hydrophila and plesiomonas shigelloides infections compared with those due to vibrio cholerae non-o1 and vibrio parahaemolyticus in bangladesh. | using data from the international centre for diarrhoeal disease research, bangladesh (icddr,b) from 1996 to 2001, we compared the clinical features of diarrhea in patients with stool specimens yielding only a. hydrophila (189 patients; 1.4% of 13,970 patients screened) or p. shigelloides (253 patients) compared to patients with sole v. cholerae non-o1 infection (99 patients) or v. parahaemolyticus infection (126 patients). patients exhibited similar frequencies of fever (temperature >37.8°c), st ... | 2012 | 23762755 |
| detection of aerolysin gene in aeromonas hydrophila isolated from fish and pond water. | aerolysin is a hemolytic toxin encoded by aerolysin gene (1482 bp) that plays a key role in the pathogenesis of aeromonas hydrophila infection in fish. new speciesspecific primers were designed to amplify 326 bp conserved region of aerolysin gene for a. hydrophila. twenty-five isolates of a. hydrophila recovered from fish and pond water were studied for detection of aerolysin gene. aerolysin gene was detected in 85% of the isolates during the study. the designed primers were highly specific and ... | 2008 | 23100746 |
| antibacterial efficacy of recombinant siganus oramin l-amino acid oxidase expressed in pichia pastoris. | siganus oraminl-amino acid oxidase is a novel natural protein (named sr-laao) isolated from serum of the rabbitfish (s. oramin), which showed antibacterial activity against both gram-positive and gram-negative bacteria and had a lethal effect on the parasites cryptocaryon irritans, trypanosoma brucei brucei and ichthyophthirius multifiliis. in order to test whether recombinant sr-laao (rsr-laao) produced by the eukaryotic expression system also has antimicrobial activity, the yeast pichia pastor ... | 2014 | 25238719 |
| bundle-forming pilus locus of aeromonas veronii bv. sobria. | little is known about the colonization mechanisms of aeromonas spp. previous work has suggested that the type iv bundle-forming pilus (bfp) is an aeromonad intestinal colonization factor. this study provides the first genetic characterization of this structure. to define the role of bfp in aeromonas veronii bv. sobria adherence, a 22-kb locus encoding the bundle-forming pilus was isolated; this contained 17 pilus-related genes similar to the mannose-sensitive hemagglutinin (msha) of vibrio chole ... | 2012 | 22311923 |
| a comparative study of cold- and warm-adapted endonucleases a using sequence analyses and molecular dynamics simulations. | the psychrophilic and mesophilic endonucleases a (enda) from aliivibrio salmonicida (vsenda) and vibrio cholera (vcenda) have been studied experimentally in terms of the biophysical properties related to thermal adaptation. the analyses of their static x-ray structures was no sufficient to rationalize the determinants of their adaptive traits at the molecular level. thus, we used molecular dynamics (md) simulations to compare the two proteins and unveil their structural and dynamical differences ... | 2017 | 28192428 |
| mutt from the fish pathogen aliivibrio salmonicida is a cold-active nucleotide-pool sanitization enzyme with unexpectedly high thermostability. | upon infection by pathogenic bacteria, production of reactive oxygen species (ros) is part of the host organism's first line of defence. ros damage a number of macromolecules, and in order to withstand such a harsh environment, the bacteria need to have well-functioning ros scavenging and repair systems. herein, mutt is an important nucleotide-pool sanitization enzyme, which degrades 8-oxo-dgtp and thus prevents it from being incorporated into dna. in this context, we have performed a comparativ ... | 2015 | 25737836 |
| the immediate global responses of aliivibrio salmonicida to iron limitations. | iron is an essential micronutrient for all living organisms, and virulence and sequestration of iron in pathogenic bacteria are believed to be correlated. as a defence mechanism, potential hosts therefore keep the level of free iron inside the body to a minimum. in general, iron metabolism is well studied for some bacteria (mostly human or animal pathogens). however, this area is still under-investigated for a number of important bacterial pathogens. aliivibrio salmonicida is a fish pathogen, an ... | 2015 | 25649684 |
| tracing enteric pathogen contamination in sub-saharan african groundwater. | quantitative pcr (qpcr) can rapidly screen for an array of faecally-derived bacteria, which can be employed as tracers to understand groundwater vulnerability to faecal contamination. a microbial dna qpcr array was used to examine 45 bacterial targets, potentially relating to enteric pathogens, in 22 groundwater supplies beneath the city of kabwe, zambia in both the dry and subsequent wet season. thermotolerant (faecal) coliforms, sanitary risks, and tryptophan-like fluorescence, an emerging rea ... | 2015 | 26363144 |
| spectroscopic investigations, antimicrobial, and cytotoxic activity of green synthesized gold nanoparticles. | the gold nanoparticles (aunps) were synthesized by using naturally available punica granatum fruit extract as reducing and stabilizing agent. the biosynthesized aunps was characterized by using uv-vis, fluorescence, high resolution transmission electron microscopy (hrtem), x-ray diffraction (xrd), fourier transform infrared (ftir) and thermogravimetric (tga) analysis. the surface plasmon resonance (spr) band at 585nm confirmed the reduction of auric chloride to aunps. the crystalline nature of t ... | 2014 | 24755638 |
| antimicrobial activity of natural respitol-b and its main components against poultry microorganisms. | poultries are infected to different kinds of microbial infections during their growth. for prevent of these diseases, many farmers use the synthetic antimicrobial agents. whereas, the poultries participate in food cycle of human, the residues of these agents enter in human and cause many undesired side effects. in this study, the antimicrobial activity of respitol-b and its main components (eucalyptus oil and menthol) was evaluated on different kinds of microorganisms including gram positive, gr ... | 2013 | 24502173 |
| antioxidative, antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract. | this study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of leea indica leaf ethanol extract. phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, dpph radical scavenging effect, fecl3 reducing power, dmso superoxide scavenging effect and iron chelating effects were studied by established methods. antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine ... | 2013 | 23961238 |
| synthesis, characterization, and antimicrobial evaluation of carbostyril derivatives of 1h-pyrazole. | a new series of 12 derivatives of 4-pyrazolyl-n-arylquinoline-2,5-dione (4a-l) were synthesized by one pot base catalyzed cyclocondensation reaction of 1-aryl-5-chloro-3-methyl-1h-pyrazole-4-carbaldehyde (1a-c), meldrum's acid (2) and 3-arylamino-5,5-disubstitutedcyclohex-2-enone (3a-d). all the compounds were characterized by elemental analysis, ft-ir, (1)h nmr and (13)c nmr spectral data and were screened, against six bacterial pathogens, namely bacillus subtilis, clostridium tetani, streptoco ... | 2011 | 23960745 |
| biosynthesis of silver nanoparticles using fresh extracts of tridax procumbens linn. | a simple and eco-friendly method for the synthesis of biogenic nanoparticles (np's) using an aqueous solution of t. procumbens fresh plant extract (leaf and stem) as a bioreductant is reported. the prepared biogenic nanoparticles were well characterized using u.v. visible spectroscopy, scanning electron microscopy, x-ray diffraction and fourier-transform infrared spectroscopy. the particles were confirmed to be elemental crystal by x-ray diffraction. the potential applications of biosynthesized ... | 2014 | 24772939 |
| production of chitosan from endolichenic fungi isolated from mangrove environment and its antagonistic activity. | to screen the chitosan producing ability of endolichenic fungi and its antibacterial activity. | 2012 | 23569884 |
| a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute. | in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ... | 2016 | 27874136 |
| dimerization of bacterial diaminopimelate decarboxylase is essential for catalysis. | diaminopimelate decarboxylase (dapdc) catalyzes the final step in the diaminopimelate biosynthesis pathway of bacteria. the product of the reaction is the essential amino acid l-lysine, which is an important precursor for the synthesis of the peptidoglycan cell wall, housekeeping proteins, and virulence factors of bacteria. accordingly, the enzyme is a promising antibacterial target. previous structural studies demonstrate that dapdc exists as monomers, dimers, and tetramers in the crystal state ... | 2016 | 26921318 |
| [optimization of labeling and localizing bacterial membrane and nucleus with fm4-64 and hoechst dyes]. | to observe cell membrane and nucleus in bacteria for subcellular localization. | 2015 | 26665605 |
| [the experiments conducted by japanese on human guinea pigs, and the use of biological weapons during the sino-japanese war (1937-1945)]. | starting from the end of the nineteenth century, and during the first four decades of the past century, japan showed considerable military expansion, on the back of a pan-asiatic and imperialistic ideology, comparable only to those expressed by wilhelmian and nazi germany. this growth led to japan playing an extremely important role in the asia-pacific continent, which unavoidably brought the country onto a collision course with the british empire and the united states of america. the japanese g ... | 2014 | 25269971 |
| haem-based sensors: a still growing old superfamily. | the haem-based sensors are chimeric multi-domain proteins responsible for the cellular adaptive responses to environmental changes. the signal transduction is mediated by the sensing capability of the haem-binding domain, which transmits a usable signal to the cognate transmitter domain, responsible for providing the adequate answer. four major families of haem-based sensors can be recognized, depending on the nature of the haem-binding domain: (i) the haem-binding pas domain, (ii) the co-sensit ... | 2013 | 24054793 |
| the papain inhibitor (spi) of streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth. | a novel papain inhibitory protein (spi) from streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (staphylococcus aureus sspb) and culture supernatants (porphyromonas gingivalis, bacillus anthracis). further, growth of bacillus anthracis, staphylococcus aureus, pseudomonas aeruginosa, and vibrio cholerae was completely inhibited by 10 μm spi. at this concentration of spi, no cytotoxicity was observed. we conclude that spi inhibits bacteria ... | 2013 | 23587952 |
| structural characterization and comparison of three acyl-carrier-protein synthases from pathogenic bacteria. | some bacterial type ii fatty-acid synthesis (fas ii) enzymes have been shown to be important candidates for drug discovery. the scientific and medical quest for new fas ii protein targets continues to stimulate research in this field. one of the possible additional candidates is the acyl-carrier-protein synthase (acps) enzyme. its holo form post-translationally modifies the apo form of an acyl carrier protein (acp), which assures the constant delivery of thioester intermediates to the discrete e ... | 2012 | 22993090 |
| evaluation of sample preparation methods for maldi-tof ms identification of highly dangerous bacteria. | to propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by maldi-tof ms. | 2012 | 22512320 |
| targeting bacterial toxins. | protein toxins constitute the main virulence factors of several species of bacteria and have proven to be attractive targets for drug development. lead candidates that target bacterial toxins range from small molecules to polymeric binders, and act at each of the multiple steps in the process of toxin-mediated pathogenicity. despite recent and significant advances in the field, a rationally designed drug that targets toxins has yet to reach the market. this review presents the state of the art i ... | 2012 | 22441768 |
| bacteria and their toxins tamed for immunotherapy. | bacterial toxins share the ability to enter host cells to target various intracellular proteins and to modulate host immune responses. over the last 20 years, toxins and their mutated variants, as well as live attenuated bacteria, have been exploited for vaccination and immunotherapy of various infectious, malignant and autoimmune diseases. the ability of bordetella pertussis adenylate cyclase toxin to translocate its adenylate cyclase domain across the host cell membrane, as well as the pathway ... | 2012 | 22339216 |
| antibacterial and cytotoxic activities of terminalia stenostachya and terminalia spinosa. | plants that belong to the combretaceae family have long history of use in the traditional medicine systems of africa and asia for treatment of diseases and conditions associated with hiv/aids-opportunistic infections. the objective of this study was to investigate the biological activities of extracts of terminalia stenostachya engl. & diels and terminalia spinosa engl. (combretaceae), to verify the rationale for their use by traditional health practitioners in the treatment of hiv/aids patients ... | 2011 | 25566609 |
| effects of ethyl acetate extracts from the polycephalomyces nipponicus isolate cod-mk1201 (ascomycetes) against human pathogenic bacteria and a breast cancer cell line. | this study aimed to identify a suitable organic solvent for extracting bioactive compounds from polycephalomyces nipponicus and to evaluate the antibacterial and anticancer activities of the extracts obtained. only extracts obtained with ethyl acetate exhibited antibacterial activity, so ethyl acetate was chosen for large-scale extraction. the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against gram-positive and gram-negative human pathogenic bacteria of t ... | 2016 | 27910791 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| the impact of oxygen on bacterial enteric pathogens. | bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ... | 2016 | 27261784 |
| antibiotic-resistant bacteria: prevalence in food and inactivation by food-compatible compounds and plant extracts. | foodborne antibiotic-resistant pathogenic bacteria such as campylobacter jejuni, bacillus cereus, clostridium perfringens, escherichia coli, salmonella enterica, staphylococcus aureus, vibrio cholerae, and vibrio parahemolyticus can adversely affect animal and human health, but a better understanding of the factors involved in their pathogenesis is needed. to help meet this need, this overview surveys and interprets much of our current knowledge of antibiotic (multidrug)-resistant bacteria in th ... | 2015 | 25856120 |
| in vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from spilanthes paniculata leaves. | the study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of spilanthes paniculata leaves for the first time widely used in the traditional treatments in bangladesh. | 2014 | 25299748 |
| antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content. | grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ... | 2014 | 24945318 |
| characterization of the cell surface properties of drinking water pathogens by microbial adhesion to hydrocarbon and electrophoretic mobility measurements. | the surface characteristics of microbial cells directly influence their mobility and behavior within aqueous environments. the cell surface hydrophobicity (csh) and electrophoretic mobility (epm) of microbial cells impact a number of interactions and processes including aggregation, adhesion to surfaces, and stability of the cells within the aqueous environments. these cell characteristics are unique to the bacterial species and are a reflection of the large diversity of surface structures, prot ... | 2014 | 24815929 |
| multiplex pcr (polymerase chain reaction) assay for detection of e. coli o157:h7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon). | the coastal aquaculture mainly shrimps constitute major export sector in bangladesh and is increasingly shaped by international trade conditions and by national responses to those stringent quality and safety standards. pcr based validated methods for detection of major bacterial pathogens in shrimp might be very useful tool for ensuring quality and safety standards of exportable shrimps. the objective of this study was to evaluate overall performance (sensitivity and specificity) of the multipl ... | 2013 | 24498789 |
| antimicrobial potency of the leaf - stalk extract of curcuma longa (linn). | the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ... | 2000 | 22557005 |
| antibacterial, anti-inflammatory and probiotic potential of enterococcus hirae isolated from the rumen of bos primigenius. | in the present study bacterial strains were isolated from the rumen fluids of bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens vibrio cholera, enterococcus faecalis, enterobacter aerogens, pseudomonas ... | 2014 | 24609495 |
| antimicrobial effects of novel peptides cot2 and sot2 derived from crocodylus siamensis and pelodiscus sinensis ovotransferrins. | in light of the increasing threat of bacterial drug resistance to human health on a global scale, research and development of antimicrobial peptides as a novel class of potent antibiotics has gained considerable attention. the present study focuses on the structural evaluation and membrane interaction of two new cationic antimicrobial peptides, cot2 and sot2, derived from siamese crocodile (crocodylus siamensis) and chinese softshell turtle (pelodiscus sinensis) ovotransferrins. here, cot1 (+3) ... | 2017 | 28159460 |
| design and synthesis of cationic antibacterial peptide based on leucrocin i sequence, antibacterial peptide from crocodile (crocodylus siamensis) white blood cell extracts. | leucrocin i is an antibacterial peptide isolated from crocodile (crocodylus siamensis) white blood cell extracts. based on leucrocin i sequence, cationic peptide, ny15, was designed, synthesized and evaluated for antibacterial activity against bacillus sphaericus tistr 678, bacillus megaterium (clinical isolate), vibrio cholerae (clinical isolate), salmonella typhi (clinical isolate), salmonella typhi atcc 5784 and escherichia coli 0157:h7. the efficacy of the peptide made from all l-amino acids ... | 2014 | 24192554 |
| isolation and screening of probiotic candidates from marron, cherax cainii (austin, 2002) gastrointestinal tract (git) and commercial probiotic products for the use in marron culture. | six strains of bacteria including bacillus mycoides (a10) and shewanella species (a12) isolated from healthy marron intestine, bacillus species (pm1), bacillus subtilis (pm3), bacillus sp. (pm4) and bacillus sp. (aq) from commercial probiotic products were investigated for probiotic potential in marron culture. antibiotic susceptibility tests indicated pm3 and pm4 were susceptible to all nine antibiotics evaluated. a10, a12 and aq were resistant to class penicillins, whereas pm1 was resistant to ... | 2015 | 24917311 |
| cationic antimicrobial peptides derived from crocodylus siamensis leukocyte extract, revealing anticancer activity and apoptotic induction on human cervical cancer cells. | known antimicrobial peptides kt2 and rt2 as well as the novel rp9 derived from the leukocyte extract of the freshwater crocodile (crocodylus siamensis) were used to evaluate the ability in killing human cervical cancer cells. rp9 in the extract was purified by a combination of anion exchange column and reversed-phase hplc, and its sequence was analyzed by mass spectrometry. the novel peptide could inhibit gram-negative vibrio cholerae (clinical isolation) and gram-positive bacillus pumilus tistr ... | 2016 | 27129462 |
| green synthesis and antimicrobial activity of silver nanoparticles using wild medicinal mushroom ganoderma applanatum (pers.) pat. from similipal biosphere reserve, odisha, india. | in the present study, green synthesis and cost effective approach of silver nanoparticles using wild medicinal mushroom ganoderma applanatum (pers.) pat. from similipal biosphere reserve, odisha, india is reported. the biosynthesised agnps were characterised using uv-visible spectroscopy, particle analyser and scanning electron microscopy studies. it was found by dynamic light scattering analysis, that the average size and charges of the agnps were 133.0 ± 0.361 nm and -6.01 ± 5.30 mv, respectiv ... | 2016 | 27463787 |
| hard-wired control of bacterial processes by chromosomal gene location. | bacterial processes, such as stress responses and cell differentiation, are controlled at many different levels. while some factors, such as transcriptional regulation, are well appreciated, the importance of chromosomal gene location is often underestimated or even completely neglected. a combination of environmental parameters and the chromosomal location of a gene determine how many copies of its dna are present at a given time during the cell cycle. here, we review bacterial processes that r ... | 2016 | 27364121 |
| use of a novel report protein to study the secretion signal of flagellin in bacillus subtilis. | flagellin (also called hag) is the main component of bacterial flagellum and is transported across the cytoplasmic membrane by flagellar secretion apparatus. because flagella play an essential role in the pathogenesis of numerous pathogens, the flagellins of escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, campylobacter jejuni, and vibrio cholerae have been intensively studied; however, very few studies have focused on the flagellin of bacillus subtilis, which is considered to b ... | 2016 | 27154466 |
| antibacterial activity of an acidic phospholipase a2 (nn-xib-pla2) from the venom of naja naja (indian cobra). | the resistance of bacteria against the use of conventional antibiotics has become a serious threat to public health and considering the associated side effect with antibiotics; new strategies to find and develop new molecules with novel modes of action has received grate attention in recent years. in this study, when the antibacterial potential of an acidic protein-nn-xib-pla2 (naja naja venom phospholipase a2 fraction-xib) of naja naja venom was evaluated, it showed significant bactericidal act ... | 2016 | 26885465 |
| a specific antimicrobial protein cap-1 from pseudomonas sp. isolated from the jellyfish cyanea capillata. | a bacterium strain, designated as cmf-2, was isolated from the jellyfish cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. the strain cmf-2 was identified as pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16s rrna sequence analysis. in this study, an antimicrobial protein, named as cap-1, was isolated from the culture of cmf-2 through ammonium sulfate precipitation and gel filtration chromatography. ... | 2016 | 26529191 |
| bark extract mediated green synthesis of silver nanoparticles: evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. | in the current investigation we report the biosynthesis potentials of bark extracts of ficus benghalensis and azadirachta indica for production of silver nanoparticle without use of any external reducing or capping agent. the appearance of dark brown color indicated the complete nanoparticle synthesis which was further validated by absorbance peak by uv-vis spectroscopy. the morphology of the synthesized particles was characterized by field emission- scanning electron microscopy (fe-sem) and ato ... | 2016 | 26478285 |
| biology of three ice families: sxt/r391, icebs1, and icest1/icest3. | integrative and conjugative elements (ices) are bacterial mobile genetic elements that play a key role in bacterial genomes dynamics and evolution. ices are widely distributed among virtually all bacterial genera. recent extensive studies have unraveled their high diversity and complexity. the present review depicts the general conserved features of ices and describes more precisely three major families of ices that have been extensively studied in the past decade for their biology, their evolut ... | 2014 | 26104437 |
| antibacterial potential of a basic phospholipase a2 (vrv-pl-viiia) from daboia russelii pulchella (russell's viper) venom. | microbial/bacterial resistance against antibiotics poses a serious threat to public health. furthermore, the side effects of these antibiotics have stimulated tremendous interest in developing new molecules from diverse organisms as therapeutic agents. this study evaluates the antibacterial potential of a basic protein, vipera russellii venom phospholipase a2 fraction viiia (vrv-pl-viiia), from daboia russelii pulchella venom against gram-positive and gram-negative bacteria. | 2015 | 26042153 |
| tracking the elusive function of bacillus subtilis hfq. | rna-binding protein hfq is a key component of the adaptive responses of many proteobacterial species including escherichia coli, salmonella enterica and vibrio cholera. in these organisms, the importance of hfq largely stems from its participation to regulatory mechanisms involving small non-coding rnas. in contrast, the function of hfq in gram-positive bacteria has remained elusive and somewhat controversial. in the present study, we have further addressed this point by comparing growth phenoty ... | 2015 | 25915524 |
| two conserved amino acids of juxtaposed domains of a ribosomal maturation protein cgta sustain its optimal gtpase activity. | cgta is a highly conserved ribosome binding protein involved in ribosome biogenesis and associated with stringent response. it is a 55 kda gtpase protein consisting of gtpase, obg and c-terminal domains. the function of the latter two domains was not clear and despite the importance, the mode of action of cgta is still largely unknown. knocking out of cgta gene is lethal and mutations lead to growth, sporulation and developmental defects in bacteria. it was found that a growth defect and pinhole ... | 2015 | 25912137 |
| graphene oxide nanoribbon-based sensors for the simultaneous bio-electrochemical enantiomeric resolution and analysis of amino acid biomarkers. | in this work, a straightforward in-situ measurement of l and d-amino acids (aas) has been developed using disposable graphene oxide nanoribbon (gon) screen printed electrodes. for that, we took advantage of the electroactivity of certain clinically relevant aas, such as tyrosine (tyr) and methionine (met), which are involved in important bacterial diseases (bacillus subtilis and vibrio cholera, respectively). the strategy is based on a dual electrochemical and enzymatic approach. the d-aa with t ... | 2015 | 25562744 |
| antibacterial potential of a basic phospholipase a2 (vrv-pl-v) of daboia russellii pulchella (russell's viper) venom. | microbial/bacterial resistance against antibiotics is considered as a potentially serious threat to public health. further, as these antibiotics elicit side effects, there is interest in developing new molecules with novel modes of action from diverse organisms. along these lines, in this study the antibacterial potential of the basic protein vrv-pl-v (vipera russellii venom phospholipase a2 fraction v) of daboia russellii pulchella venom was evaluated. vrv-pl-v demonstrated a potent antibacteri ... | 2014 | 25540009 |
| crp-dependent positive autoregulation and proteolytic degradation regulate competence activator sxy of escherichia coli. | natural competence, the ability of bacteria to take up exogenous dna and incorporate it into their chromosomes, is in most bacteria a transient phenomenon under complex genetic and environmental control. in the gram-negative bacteria haemophilus influenzae and vibrio cholerae, the master regulator sxy/tfox controls competence development. although not known to be naturally competent, escherichia coli possesses a sxy homologue and a competence regulon containing the genes required for dna uptake. ... | 2015 | 25491382 |
| biosynthesis, characterization and antimicrobial activity of silver nanoparticles by streptomyces sp. ss2. | in the present study the microbial biosynthesis of silver nanoparticles (agnps) by secondary metabolites of streptomyces sp. ss2 in an eco-friendly approach has been reported. the streptomyces sp. ss2 was isolated from the soil sediment of similipal biosphere reserve. the identification of this strain was determined by phenotypical characteristics (morphological and biochemical) and molecular characterization method using 16 s rdna sequencing. the morphological study was also done by high-resolu ... | 2014 | 24842223 |
| enzymatic sequencing of partially acetylated chitosan oligomers. | chitosan oligosaccharides have diverse biological activities with potentially valuable applications, for example, in the fields of medicine and agriculture. these functionalities are thought to depend on their degree of polymerization and acetylation, and possibly on specific patterns of acetylation. chitosan oligomers with fully defined architecture are difficult to produce, and their complete analysis is demanding. analysis is typically done using ms or nmr, requiring access to expensive infra ... | 2014 | 24824785 |
| potentialities of newly isolated bacillus subtilis and lactobacillus sp for curd preparation and a comparative study of its physico-chemical parameters with other marketed curds. | two bacillus sp. were isolated from the local fermented milk and identified on the basis 16s rrna sequence profile as bacillus subtilis akl1 and by biochemical process as lactobacillus acidophilus akl2. these isolates were used as fresh inoculums for curd preparation individually and in combinations. different physico-chemical and therapeutic properties of the newly prepared curd were examined and compared with marketed local (sweet and sour) and branded (mother dairy and thackar) curds. the tot ... | 2013 | 24416925 |
| microalgae mediated synthesis of silver nanoparticles and their antibacterial activity against pathogenic bacteria. | silver nanoparticles is known to have antimicrobial affects. cyanobacteria isolates from muthupet mangrove includes aphanothece sp, oscillatoria sp, microcoleus sp, aphanocapsa sp, phormidium sp, lyngbya sp, gleocapsa sp, synechococcus sp, spirulina sp with were set in compliance with their cellular mechanism of nano silver creation, and were investigated by uv-vis spectrophotometer, energy-dispersive x-ray (edx) and scanning electron microscopy (sem). silver nanoparticles were spherical shaped ... | 2013 | 23821828 |
| microalgae associated brevundimonas sp. msk 4 as the nano particle synthesizing unit to produce antimicrobial silver nanoparticles. | the biosynthesis of silver nanoparticles and its antimicrobial property was studied using bacteria isolated from spirulina products. isolated bacteria were identified as bacillus sp. msk 1 (jx495945), staphylococcus sp. msk 2 (jx495946), bacillus sp. msk 3 (jx495947) and brevundimonas sp. msk 4 (jx495948). silver nanoparticles (agnps) were synthesized using bacterial culture filtrate with agno3. the initial syntheses of ag nanoparticles were characterized by uv-vis spectrophotometer (by measurin ... | 2013 | 23711394 |
| an efficient domino reaction in ionic liquid: synthesis and biological evaluation of some pyrano- and thiopyrano-fused heterocycles. | an improved domino/knoevenagel-hetero-diels-alder reaction of two new aldehyde substrates; 7-olefinoxy-coumarin-8-carbaldehyde and 2-alkensulfanyl-quinoline-3-carbaldehyde, with pyrazolones was studied in ionic liquid triethylammonium acetate (teaa), affording a series of pyrazolopyran annulated-pyrano-fused coumarins, and thiopyrano-fused quinolones. besides acting as catalyst, since no additional catalyst used, the ionic liquid teaa also promised its easy recovery. in all new polyheterocycles, ... | 2013 | 23414843 |
| modulation of quaternary structure and enhancement of ligand binding by the k-turn of tandem glycine riboswitches. | most known glycine riboswitches have two homologous aptamer domains arranged in tandem and separated by a short linker. the two aptamers associate through reciprocal "quaternary" interactions that have been proposed to result in cooperative glycine binding. recently, the interaptamer linker was found to form helix p0 with a previously unrecognized segment 5' to the first aptamer domain. p0 was shown to increase glycine affinity, abolish cooperativity, and conform to the k-turn motif consensus. w ... | 2013 | 23249744 |
| analysis toward innovative herbal antibacterial and antifungal drugs. | the antimicrobial activities of four medicinal plants argemona mexicana, achyranthes aspera, catharanthus roseus, and syzygium cumini were evaluated against escherichia coli, vibrio cholerae, klebsiella pneumoniae, proteus vulgaris, bacillus subtilis, salmonella typhi and three aspergillus species. extracts from achyranthes aspera and catharanthus roseus showed the highest antimicrobial potential (mic 0.375-0.750 mg/ml) while extract from argemona mexicana and syzygium cumini, showed less activi ... | 2012 | 23072646 |
| antibacterial activities of polyethylene glycol, tween 80 and sodium dodecyl sulphate coated silver nanoparticles in normal and multi-drug resistant bacteria. | antibacterial activity of silver nanoparticles coated with different functionalizing agents i.e., polyethylene glycol, tween 80 and sodium dodecyl sulphate were evaluated on both normal and multi-drug resistant strains of bacteria. under the same reaction conditions, these functionalizing agents were added separately to coat silver nanoparticles. among these, polyethylene glycol coated nanoparticles were most effective in killing all the bacterial strains which includes escherichia coli dh5a, ba ... | 2012 | 22755083 |
| antibiofilm activity of α-amylase from bacillus subtilis s8-18 against biofilm forming human bacterial pathogens. | the extracellular α-amylase enzyme from bacillus subtilis s8-18 of marine origin was proved as an antibiofilm agent against methicillin-resistant staphylococcus aureus (mrsa), a clinical strain isolated from pharyngitis patient, vibrio cholerae also a clinical isolate from cholera patient and pseudomonas aeruginosa atcc10145. the spectrophotometric and microscopic investigations revealed the potential of α-amylase to inhibit biofilm formation in these pathogens. at its bic level, the crude enzym ... | 2012 | 22350933 |
| occurrence of ferredoxin:nad(+) oxidoreductase activity and its ion specificity in several gram-positive and gram-negative bacteria. | a ferredoxin:nad(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium acetobacterium woodii. the enzyme is assumed to be encoded by the rnf genes. since these genes are present in the genomes of many bacteria, we tested for ferredoxin:nad(+) oxidoreductase activity in cytoplasmic membranes from several different gram-positive and gram-negative bacteria that have annotated rnf genes. we found this activity in clostridium tetanomorphum, clostr ... | 2016 | 26793417 |
| effects of the food manufacturing chain on the viability and functionality of bifidobacterium animalis through simulated gastrointestinal conditions. | the viability and functionality of probiotics may be influenced by industrial production processes resulting in a decrease in probiotic efficiency that benefit the health of humans. this study aimed to investigate the probiotic characteristics of bifidobacterium strains isolated from fecal samples of healthy thai infants. in the present work, three local strains (bf014, bf052, and bh053) belonging to bifidobacterium animalis showed a great resistance against conditions simulating the gastrointes ... | 2016 | 27333286 |
| removal of cholera toxin from aqueous solution by probiotic bacteria. | cholera remains a serious health problem, especially in developing countries where basic hygiene standards are not met. the symptoms of cholera are caused by cholera toxin, an enterotoxin, which is produced by the bacterium vibrio cholerae. we have recently shown that human probiotic bacteria are capable of removing cyanobacterial toxins from aqueous solutions. in the present study we investigate the ability of the human probiotic bacteria, lactobacillus rhamnosus strain gg (atcc 53103) and bifi ... | 2012 | 24281668 |
| case-control study of diarrheal disease etiology in individuals over 5 years in southwest china. | acute diarrhea is one of the major public health problems worldwide. most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in china. | 2016 | 27891182 |
| impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest china. | acute diarrhea is a global health problem, resulting in high morbidity and mortality in children. it has been suggested that enteric pathogen co-infections play an important role in gastroenteritis, but most research efforts have only focused on a small range of species belonging to a few pathogen groups. this study aimed to assess the impact of co-infections with a broad range of enteric pathogens on children aged below five years who suffer from acute diarrhea in southwest china. | 2016 | 27349521 |
| establishing a list of qualifying pathogens under the food and drug administration safety and innovation act. final rule. | the food and drug administration (fda or agency) is issuing a regulation to establish a list of "qualifying pathogens'' that have the potential to pose a serious threat to public health. this final rule implements a provision of the generating antibiotic incentives now (gain) title of the food and drug administration safety and innovation act (fdasia). gain is intended to encourage development of new antibacterial and antifungal drugs for the treatment of serious or life-threatening infections, ... | 2014 | 24908687 |
| inactivation of vegetative bacterial threat agents on environmental surfaces. | following a wide-area biological terror attack, numerous decontamination technologies, techniques, and strategies will be required for rapid remediation. establishing an understanding of how disinfectants will perform under field conditions is of critical importance. the purpose of this study was to determine the efficacy of several liquid decontaminants, when used to inactivate vegetative biological agents on environmental surfaces. aluminum, carpet, concrete, glass, and wood coupons were inocu ... | 2013 | 23208274 |
| characterization of exogenous bacterial oligosaccharyltransferases in escherichia coli reveals the potential for o-linked protein glycosylation in vibrio cholerae and burkholderia thailandensis. | bacterial protein glycosylation systems from varying species have been functionally reconstituted in escherichia coli. both n- and o-linked glycosylation pathways, in which the glycans are first assembled onto lipid carriers and subsequently transferred to acceptor proteins by an oligosaccharyltransferase (otase), have been documented in bacteria. the identification and characterization of novel otases with different properties may provide new tools for engineering glycoproteins of biotechnologi ... | 2012 | 22391990 |
| development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. | childhood diarrhoea can be caused by many pathogens that are difficult to assay in the laboratory. molecular diagnostic techniques provide a uniform method to detect and quantify candidate enteropathogens. we aimed to develop and assess molecular tests for identification of enteropathogens and their association with disease. | 2014 | 25022434 |
| unique features of the motility and structures in the flagellate polar region of campylobacter jejuni and other species: an electron microscopic study. | similarly to helicobacter pylori but unlike vibrio cholerae o1/o139, campylobacter jejuni is non-motile at 20°c but highly motile at ≥37°c. the bacterium c. jejuni has one of the highest swimming speeds reported (>100 μm/s), especially at 42°c. straight and spiral bacterial shapes share the same motility. c. jejuni has a unique structure in the flagellate polar region, which is characterized by a cup-like structure (beneath the inner membrane), a funnel shape (opening onto the polar surface) and ... | 2013 | 23252968 |
| inhibition of adhesion of intestinal pathogens (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) by common oligosaccharides. | inhibition of the binding of pathogenic adhesins to host glycans by suitable oligosaccharides forms the basis of antiadhesion therapies. experiments were carried out to study the inhibition capability of oligosaccharides on the adhesion of four microorganisms (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) to ht-29 cells. results showed that, in the absence of oligosaccharides, all of the four pathogens efficiently adhered to the cells. cell adhesion with di ... | 2015 | 25692734 |
| antibacterial and antioxidant activities in extracts of fully grown cladodes of 8 cultivars of cactus pear. | the antimicrobial and antioxidant activities of some cultivars of the nopal cactus have not been determined. in this study, 8 cultivars of nopal cacti from mexico were assayed for phenolic content, antioxidant activities, and antimicrobial activities against campylobacter jejuni, vibrio cholera, and clostridium perfringens. plant material was washed, dried, and macerated in methanol. minimum bactericidal concentrations (mbcs) were determined using the broth microdilution method. antioxidant acti ... | 2014 | 24621296 |
| development of a magnetic nanoparticles microarray for simultaneous and simple detection of foodborne pathogens. | foodborne diseases are a widespread and growing public health problem affecting both developed and developing countries, microbiologically contaminated food and water are the major causes of diarrhoeal diseases. methods based on polymerase chain reaction (pcr) and microarrays are rapid and sensitive enough to detect very small quantities of microorganisms, however, the requirement for expensive equipments limits their application. in the present paper, we describe a method based on multiplex pcr ... | 2013 | 23909141 |
| burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the global enteric multicenter study, gems): a prospective, case-control study. | diarrhoeal diseases cause illness and death among children younger than 5 years in low-income countries. we designed the global enteric multicenter study (gems) to identify the aetiology and population-based burden of paediatric diarrhoeal disease in sub-saharan africa and south asia. | 2013 | 23680352 |
| a polymer microfluidic chip for quantitative detection of multiple water- and foodborne pathogens using real-time fluorogenic loop-mediated isothermal amplification. | inexpensive, portable, and easy-to-use devices for rapid detection of microbial pathogens are needed to ensure safety of water and food. in this study, a disposable polymer microfluidic chip for quantitative detection of multiple pathogens using isothermal nucleic acid amplification was developed. the chip contains an array of 15 interconnected reaction wells with dehydrated primers for loop-mediated isothermal amplification (lamp), and requires only a single pipetting step for dispensing of sam ... | 2012 | 22566273 |
| structural basis for competitive inhibition of 3,4-dihydroxy-2-butanone-4-phosphate synthase from vibrio cholerae. | the riboflavin biosynthesis pathway has been shown to be essential in many pathogens and is absent in humans. therefore, enzymes involved in riboflavin synthesis are considered as potential antibacterial drug targets. the enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (dhbps) catalyzes one of the two committed steps in the riboflavin pathway and converts d-ribulose 5-phosphate (ru5p) to l-3,4-dihydroxy-2-butanone 4-phosphate and formate. moreover, dhbps is shown to be indispensable for myc ... | 2015 | 25792735 |
| broad spectrum anti-microbial compounds producing bacteria from coast of qingdao bays. | anti-microbial resistance burden and hazard associated with chemical treatment of infections demanded for new anti-microbial natural products. marine associated microorganisms are the enormous source of bioactive compounds. in this study we have isolated 272 marine bacteria among them 136 (50%) were antagonistic to at least one of the four pathogenic strains listeria monocytogenes, vibrio cholerae, e. coli and s. aureus. only two strains exhibited antibacterial activity against all four test str ... | 2015 | 25730803 |
| the preliminary assessment of anti-microbial activity of hplc separated components of kirkia wilmsii. | most communities in developing countries rely on traditional medicines for the treatment of diseases. in south africa, the limpopo province, within the lebowakgomo district, uses tuberous roots of kirkia wilmsii, after infusion in water for the treatment of a wide range of diseases by sotho communities. | 2014 | 25371593 |
| 1-alkyl-(n,n-dimethylamino)pyridinium bromides: inhibitory effect on virulence factors of candida albicans and on the growth of bacterial pathogens. | a homologous series of 1-alkyl-(n,n-dimethylamino)pyridinium bromides, termed compounds 1-11, was synthesized and studied for antibacterial and antifungal activity. of these, compound 8, containing a ten-carbon alkyl chain, showed maximum inhibition against all the tested bacterial strains. the highest antibacterial activity using a disc diffusion method was recorded against mycobacterium smegmatis [zone of inhibition (zoi): 45.75±0.25 mm], followed by escherichia coli, proteus mirabilis, vibrio ... | 2013 | 23118472 |
| recent patents on antibacterial, antifungal and antiviral properties of tea. | teas have beneficial effects on human health including cardioprotective, anticarcinogenic, antibacterial, antiviral and antifungal activity. the precise antimicrobial spectrum of tea is difficult to be defined due to variation in the methods of testing that have been used. antibacterial effects of tea have been demonstrated against a number of microorganisms including staphylococcus aureus, vibrio cholerae, escherichia coli, shigella spp., salmonella spp., bacillus spp., klebsiella spp. and pseu ... | 2012 | 22353001 |
| poly(amidoamine) dendrimers show carbonic anhydrase inhibitory activity against α-, β-, γ- and η-class enzymes. | four generations of poly(amidoamine) (pamam) dendrimers incorporating benzenesulfonamide moieties were investigated as inhibitors of carbonic anhydrases (cas, ec 4.2.1.1) belonging to the α-, β-, γ- and η-classes which are present in pathogenic bacteria, fungi or protozoa. the following bacterial, fungal and protozoan organisms were included in the study: vibrio cholerae, trypanosoma cruzi, leishmania donovani chagasi, porphyromonas gingivalis, cryptococcus neoformans, candida glabrata, and plas ... | 2015 | 26476668 |
| microbiology, infection control and infection related outcome in pediatric patients in an oncology center in eastern india: experience from tata medical center, kolkata. | infection is a major determinant in the outcome of patients with cancer. | 2016 | 26842142 |
| comparative evaluation of the protective efficacy of two formulations of a recombinant chlamydia abortus subunit candidate vaccine in a mouse model. | chlamydia abortus (c. abortus) is the causative agent of ovine enzootic abortion (oea) and poses a zoonotic risk to pregnant women. current live attenuated 1b vaccines are efficacious but cause disease in vaccinated animals and inactivated vaccines are only marginally protective. we tested the ability of a new c. abortus subunit vaccine candidate based on the conserved and immunogenic polymorphic membrane protein d (pmp18d) formulated in cpg1826+fl (fms-like tyrosine kinase 3 ligand; flt3l) or v ... | 2015 | 25698486 |
| interferon-inducible guanylate-binding proteins at the interface of cell-autonomous immunity and inflammasome activation. | guanylate-binding proteins (gbps) are essential components of cell-autonomous immunity. in response to ifn signaling, gbps are expressed in the cytoplasm of immune and nonimmune cells, where they unleash their antimicrobial activity toward intracellular bacteria, viruses, and parasites. recent studies have revealed that gbps are essential for mediating activation of the caspase-1 inflammasome in response to the gram-negative bacteria salmonella enterica serovar typhimurium, francisella novicida, ... | 2017 | 27418355 |