| vibrio alginolyticus in the u.k. | | 1991 | 1753130 |
| the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. | chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ... | 1992 | 1594593 |
| variations in rrna content of marine vibrio spp. during starvation-survival and recovery. | the degree and temporal context of variations in ribosome content during nutrient starvation of two copiotrophic marine bacteria, vibrio alginolyticus and vibrio furnissii, have been examined. the organisms were starved either by nutritional shift-down or by consumption of limiting nutrients resulting from growth into stationary phase. measurements of the amount of hybridization to 16s rrna-specific probes revealed that the cells retained between 10 and 26% of their original rrna content after 1 ... | 1992 | 1371659 |
| roles of k+ and na+ in ph homeostasis and growth of the marine bacterium vibrio alginolyticus. | the marine bacterium vibrio alginolyticus, containing 470 mm-k+ and 70 mm-na+ inside its cells, was able to regulate the cytoplasmic ph (ph(in)) in the narrow range 7.6-7.8 over the external ph (ph(out)) range 6.0-9.0 in the presence of 400 mm-na+ and 10 mm-k+. in the absence of external k+, however, phin was regulated only at alkaline ph(out) values above 7.6. when the cells were incubated in the presence of unusually high k+ (400 mm) and 4 mm na+, the ph(in) was regulated only at acidic ph(out ... | 1992 | 1326594 |
| catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus. | cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ... | 1992 | 1328162 |
| incidence of toxigenic vibrios in foods available in taiwan. | a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ... | 1992 | 1399913 |
| [vibrio alginolyticus and swimmer's otitis externa. 2 cases and review of the literature]. | we describe two patients with acute diffuse external otitis (swimmer's otitis) acquired in the mediterranean shore, with vibrio alginolyticus recovered from ear fluid. we describe the biochemical profile and sensitivity pattern (mic) of both strains, comparing it to previously published data. a literature review was also performed, in which we found evidence for increasing concern of v. alginolyticus as an human pathogen. also there is a need for considering halophilic vibrio as potential pathog ... | 1991 | 1822155 |
| characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins. | irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ... | 1992 | 1406279 |
| [vibrio alginolyticus in clinical specimens]. | | 1992 | 1450271 |
| [membrane atpase of vibrio alginolyticus. ion transport activity and homology with f0f1-atpase from e. coli]. | f0f1-atpase has been isolated from the marine alkali-resistant bacterium vibrio alginolyticus. the enzyme subunits cross-reacted with antibodies against subunits alpha, beta, gamma, epsilon, and b of e. coli atpase. the purified atpase was reconstituted into liposomes effecting an atp-dependent uptake of h+. proton transport was inhibited by the atpase blockers dccd, triphenyltin, and venturicidin. na+ ions had no effect on atp-dependent proton transport. no atp-dependent transport of na+ was de ... | 1992 | 1457596 |
| towards a phylogeny of the genus vibrio based on 16s rrna sequences. | the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ... | 1992 | 1371064 |
| expression of bacillus amyloliquefaciens amylase and vibrio alginolyticus protease a fusion genes. | previously we reported [deane, s. m., maharaj, r., robb, f. t. & woods, d. r. (1987) journal of general microbiology 133, 2295-2302] that the production of a vibrio alginolyticus sds-resistant alkaline serine protease (pro a) cloned in escherichia coli was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active pro a. replacement of the v. alginolyticus promoter region by the alpha-amylase promoter region from bacillus amyloliquefaciens resulted in th ... | 1992 | 1373436 |
| mapping of antigenic sites to monoclonal antibodies on the primary structure of the f1-atpase beta subunit from escherichia coli: concealed amino-terminal region of the subunit in the f1. | to analyze relationships between the ternary and primary structures of the beta subunit of escherichia coli f1 atpase, we prepared two monoclonal antibodies beta 12 and beta 31 against the beta peptide. these antibodies bind to the beta subunit but do not bind to the f1 atpase, resulting in no inhibition of the atpase activities. several different portions of the beta subunit peptide were prepared by constructing expression plasmids carrying the corresponding dna segment of the beta subunit gene ... | 1992 | 1373594 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| vibrio alginolyticus ("achromobacter") collagenase: biosynthesis, function and application. | bacterial collagenase from aerobic non-pathogenic vibrio alginolyticus chemovar iophagus ("achromobacter" collagenase, ec 3.4.24.08) is an inducible extracellular metallo-proteinase. production of vibrio collagenase is induced specifically by collagen or by its macromolecular fragments. on the cell surface is expressed a specific receptor recognizing collagen structure. the study of natural inducers led to synthetic peptides with inducing properties. vibrio collagenase cleaves collagen helical c ... | 1992 | 1480012 |
| lethal effect of fresh sea water on vibrio parahaemolyticus and isolation of bdellovibrio parasitic against the organism. | halophilic bdellovibrio, which is parasitic and lytic to vibrio pharahaemolyticus, was ioslated from fresh sea water in the winter. it had a lethal effect on v. parahaemolyticus. the optimum temperature ofr multiplication ranged from 25 c to 30 c and growth was not observed at 35 c. plaque numbers of the isolate reached a maximum in 17 hr under conditions of shaking at 25 c in autoclaved sea water supplemented with v. parahaemolyticus cells, and were as high as ten times the number of host cells ... | 1975 | 1202252 |
| isolation of vibrio parahaemolyticus and vibrio alginolyticus from estuarine areas of southeastern alaska. | the first reported isolations of halophilic vibrios, including vibrio parahaemolyticus, from three seafood processing areas in southeastern alaska are described. | 1975 | 1124924 |
| proceedings: influences of the septum on the hippocampal dentate area which are unaccompanied by field potentials. | | 1975 | 1151852 |
| isolation of vibrio alginolyticus from wound infections. | vibrio alginolyticus was isolated from two patients with leg wounds. both had a history of recent exposure to ocean water. | 1975 | 1206111 |
| common morphogenetic aspects of various organotypic microvascular patterns. | | 1975 | 1214601 |
| [salmonellosis carrier state]. | | 1975 | 1214656 |
| isolation of vibrio alginolyticus from blood cultures in a leukaemic patient after consumption of oysters. | | 1991 | 1794376 |
| na(+)-coupled alternative to h(+)-coupled primary transport systems in bacteria. | protons are the most common coupling ions in bacterial energy conversions. however, while many organisms, such as the alkaliphilic bacilli, employ h(+)-bioenergetics for electron transport phosphorylation, they use na+ as the coupling ion for transport and flagellar movement. the na+ gradient required for these bioenergetic functions is established by the secondary na+/h+ antiporter. in contrast, vibrio alginolyticus and methanogenic bacteria have primary pumps for both h+ and na+. they use the ... | 1991 | 1665692 |
| molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria. | sucrose-positive derivatives of escherichia coli k-12, containing the plasmid pur400, and of klebsiella pneumoniae hydrolyse intracellular sucrose 6-phosphate by means of an invertase into d-glucose 6-phosphate and free d-fructose. the latter is phosphorylated by an atp-dependent fructokinase (gene scrk of an scr regulon) to d-fructose 6-phosphate. the lack of scrk does not cause any visible phenotype in wild-type strains of both organisms. using genes and enzymes normally involved in d-arabinit ... | 1991 | 1809835 |
| regulation of the mouse mammary tumor virus (mmtv) binding site in cultured mammary tissue. | the mouse mammary tumor virus (mmtv) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. the hormonal regulation of this protein was studied both in vitro and in vivo. in mammary gland explant culture, levels of the mmtv binding protein were maintained only when prolactin was present in the medium. the further addition of progesterone induced levels to 164% of controls, while estradiol was without effect. these results were reproduce ... | 1991 | 1665450 |
| the sodiummotive nadh-ubiquinone oxidoreductase of vibrio alginolyticus. | | 1991 | 1783108 |
| longitudinal study of epstein-barr virus genotypes associated with infectious mononucleosis patients and healthy carriers. | | 1990 | 2169503 |
| two generalized transducing phages in vibrio parahaemolyticus and vibrio alginolyticus. | two bacteriophages named phi vp253 and phi vp143 isolated after ultraviolet induction from lysogenic strains of vibrio parahaemolyticus have been shown to be generalized transducing phages. so far, seven different auxotrophic markers of a v. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2 x 10(-7) to 7.5 x 10(-5) per infected cell at the m.o.i. of approximately 1.0. the phage phi vp143, but not phi vp253, lysed 20 of the 28 strains of v. alginolyticus and the occu ... | 1991 | 1808461 |
| the effect of f0 inhibitors on the vibrio alginolyticus membrane atpase. | the inhibition of membrane atpase from the marine alkalotolerant bacterium vibrio alginolyticus by dccd, triphenyltin and venturicidin was studied. dccd proved to be an irreversible inhibitor, while venturicidin and triphenyltin produced a reversible inhibitory effect. the dccd-binding proteolipid was identified in the membrane preparations. the effect of the inhibitors on atpase activity and atp-dependent na(+)-transport in v. alginolyticus subcellular vesicles is discussed. | 1991 | 1826482 |
| nucleotide sequence and analysis of the vibrio alginolyticus sucrose uptake-encoding region. | the nucleotide sequence of the vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scra and scrk. the scra gene encodes an enzyme iisucrose (eiiscr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrk gene encodes a fructokinase. the deduced amino acid (aa) sequence for the v. alginolyticus eiiscr protein was homologous with the eiiscr proteins from streptococcus mutans, salmonella typhimurium (pur400 system) and bacillus s ... | 1990 | 2174811 |
| fluorescence quenching studies on the characterization of energy generated at the nadh:quinone oxidoreductase and quinol oxidase segments of marine bacteria. | generation of membrane potential (inside-positive) and delta ph (inside-acidic) at two kinds of nadh:quinone oxidoreductase segments, the na(+)-motive segment and another segment, of vibrio alginolyticus was examined by monitoring the quenching of fluorescence of oxonol v and that of quinacrine, respectively, with inside-out membrane vesicles. transient generation of membrane potential at the segment occurred when ubiquinone-1 was added in the presence of kcn and nadh. the membrane potential was ... | 1991 | 1907969 |
| [identification of species of microorganisms of the genus vibrio]. | during 1988, a study was made on 61 microorganisms, genus vibrio, which were received at the national reference laboratory for acute diarrheic diseases. pedro kouri institute of tropical medicine. of them, 46 were from children with acute diarrheic disease and 15 were isolated from the environment. by means of biochemical tests. 61 vibrio cholerae no. 01. 9 vibrio parahaemolyticus and 1 vibrio alginolyticus were identified. emphasis is placed upon the importance of keeping a systematic surveilla ... | 1991 | 1812527 |
| effect of systemic inhibition of prostaglandin synthesis on muscle protein balance after trauma in the rat. | anaesthetized rats were subjected to a single impact trauma to the medial aspect of the right hindlimb (gastrocnemius muscle), and were compared with sham-treated controls. for 3 days after injury, muscles of the traumatized limb showed a marked catabolic response. muscle protein repletion commenced after day 3, however, this process was not complete until 21 days after injury. muscles of the uninjured limb of the traumatized rats also showed a distinct catabolic response, compared with rats tha ... | 1991 | 1913329 |
| detection of antigens in urine of patients with acute falciparum and vivax malaria infections. | using an antigen-capture, dot-blot assay, antigens were detected in the urine of 50 patients infected with plasmodium falciparum. antigens were also detected in 12/15 patients who had no detectable parasitemias 1-2 weeks after chemotherapy. by western blotting and immunoprecipitation, four predominant antigens were identified with the following molecular masses (mr) and isoelectric points (pi): antigen 1, 200 kda, pi 6.4-6.27; antigen 2, 180 kda, pi 5.2-4.8; antigen 3, 150 kda, pi 5.5; antigen 4 ... | 1991 | 1951853 |
| structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6. | we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ... | 1991 | 1828805 |
| complexity and sequence identification of 24 rat v beta genes. | twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ... | 1991 | 1828824 |
| purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine in vibrio alginolyticus. | carboxynorspermidine synthase, mediates the nicotinamide-nucleotide-linked reduction of the schiff base h2n(ch2)3n = chch2ch(nh2)cooh. this is formed from l-aspartic beta-semialdehyde (asa) and 1,3-diaminopropane (dap) and is reduced to carboxynorspermidine [h2n(ch2)3nh(ch2)2ch(nh2)cooh], an intermediate in the novel pathway for norspermidine (nspd) biosynthesis. the enzyme was purified to apparent homogeneity from vibrio alginolyticus and characterized. the overall purification was about 1800-f ... | 1991 | 1955861 |
| [creatinine clearance as the foundation for clinical determination of kidney function in swine]. | comparative examinations of renal function using inulin- and endogenous creatinine-clearance tests in 5 pigs showed nearly identical values of glomerular filtration rate (gfr). based on this relation 79 healthy pigs, weighting 2 kg to 230 kg were investigated using the creatinine-clearance test for determination of normal values of gfr and urine flow rate (vu) as well as renal excretion (e), renal clearance (clr) and fractional excretion (fe) of urea, sodium, potassium, calcium, phosphorus, gluc ... | 1991 | 1948984 |
| molecular cloning and partial dna sequencing of the collagenase gene of vibrio alginolyticus. | dna fragments vibrio alginolyticus chemovar iophagus, at least 7 kb in length, were ligated to escherichia coli expression vectors. three clones of escherichia coli hb101 (plco-1, plco-2, plco-3) were obtained by the colony immunoblotting method using anti-collagenase antibody. in escherichia coli, all these genes produced collagenase antigens which were detected with western blotting. the amino acid sequence of chemically purified collagenase fragments was also analyzed. an approximately 2.5 kb ... | 1990 | 1965912 |
| development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans, fish, and shellfish. | monoclonal antibodies (mabs) against vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. the pathogens included vibrio alginolyticus, v. anguillarum, v. carchariae, v. cholerae, v. damsela, v. furnissii, v. harveyi, v. ordalii, v. parahaemolyticus, and v. vulnificus. three types of mabs were selected. the first important group included mabs that reacted with only a single species. a second group comprised a number of mabs that reacted w ... | 1992 | 1482190 |
| characteristic differences in the mode of quinone reduction and stability between energy-coupled and -uncoupled nadh-quinone reductases from bacterial respiratory chain. | bacterial respiratory chain has two types of nadh-quinone reductase (nqr): one is energy-coupled (type-1) and the other had no energy-transducing capacity, that is, energy-uncoupled (type-2). each of the nadh-reacting flavoprotein subunits of nqr-1 from escherichia coli and the marine vibrio alginolyticus reduced quinone to semiquinone radicals by the one-electron transfer pathway and was very sensitive to preincubation with nadh. on the other hand, the nqr-2 from these bacteria reduced quinone ... | 1992 | 1628743 |
| interleukin-1 receptor antagonist protein inhibits interleukin-8 expression in lipopolysaccharide-stimulated human whole blood. | interleukin-8 (il-8) is a neutrophil and lymphocyte chemoattractant and activator that may play an important role in mediating events at sites of inflammation. il-8 is produced by many cell types in response to a variety of inducers, including interleukin-1 (il-1). studies were conducted to address the question of whether an inhibitor of il-1 action, il-1 receptor antagonist protein (irap), would suppress il-8 production. lipopolysaccharide (lps)-stimulated human whole blood was used as an ex vi ... | 1992 | 1533750 |
| yolk degradation in tick eggs: ii. evidence that cathepsin l-like proteinase is stored as a latent, acid-activable proenzyme. | cathepsin l-like proteinase found in the eggs of the tick ornithodoros moubata is latent during embryogenesis, but can be activated by acid treatment. in crude extracts as well as in partially purified fractions, activation requires reducing conditions and is inhibited by leupeptin, which indicates that it is mediated by a thiol proteinase, probably by the cathepsin l itself. latency disappears in vivo at the time of the acute phase of yolk digestion, which takes place during late embryonic deve ... | 1990 | 2134179 |
| [changes in centrally released arginine vasopressin by stimulation of the medullary reticular formation]. | it has been reported that after 40 minutes of stimulation of the medullary reticular formation (morf), widespread significant increase by 1.4% to 2.8% in brain water content occurs in white matter of the injured hemisphere. recent studies indicate that centrally released arginine vasopressin (avp) influences water permeability of the brain in both normal and pathological conditions. the present study was carried out to clarify the effect of electrical stimulation of morf on centrally released av ... | 1992 | 1562384 |
| properties of respiratory chain-linked na(+)-independent nadh-quinone reductase in a marine vibrio alginolyticus. | the respiratory chain of a marine vibrio alginolyticus contains two types of nadh-quinone reductase (nqr): one is an na(+)-dependent nqr functioning as an na+ pump (nqr-1) and the other is an na(+)-independent nqr (nqr-2). nqr-2 was purified about 55-fold from the membrane of mutant nap-1 which is devoid of nqr-1, and its properties were compared with those of nqr-1. in contrast to nqr-1, the purified nqr-2 does not require any salts for activity and is not inhibited by up to 0.4 m salts. the op ... | 1992 | 1543699 |
| f0f1-atpase from vibrio alginolyticus. subunit composition and proton pumping activity. | an f0f1-atpase was isolated from the membranes of the marine bacterium vibrio alginolyticus. homology between the subunits of the f0-complexes from e. coli and v. alginolyticus was found using antibodies against subunits a, b and c of the e. coli f0f1-atpase. the f0f1-complex from v. alginolyticus was reconstituted into proteoliposomes, which were competent in atp-dependent proton uptake. this process was inhibited by triphenyltin, dccd, and venturicidin. na+ did not affect proton translocation. | 1991 | 1647986 |
| sequence of subunit c of the sodium ion translocating adenosine triphosphate synthase of propionigenium modestum. | the 30 n-terminal amino acid residues of the purified atpase c subunit of propionigenium modestum have been determined. an oligonucleotide mixture was derived from this sequence and used as probe for cloning the corresponding gene in escherichia coli. the nucleotide sequence of the gene has been determined and compared with those of atpase c subunits from other bacteria and chloroplasts. peculiar sequence similarities are found only at the c-terminus between the c subunits of the atpases from p. ... | 1990 | 2146118 |
| [isolation of vibrio alginolyticus from a leg ulcer]. | an organism presenting the characteristics of vibrio alginolyticus was isolated from a cutaneous leg ulcer in a 67-year-old woman. the origin of this halophilic bacterium remains unknown. the clinical observation and the bacteriological findings are presented and commented. | 1990 | 2161606 |
| effects of respiratory activity on starvation survival of marine vibrios. | the marine bacterium vibrio fluvialis nctc11328 responded to nutrient depletion by a reduction in cell volume, and this was prevented by conditions that eliminated respiration as a source of energy. addition of the protonophore, cccp, removal of oxygen and introduction of mutations leading to defects of the respiratory chain prevented size reduction during periods of nutrient limitation. further, survival of the wild-type strain during starvation was reduced under anaerobic conditions and surviv ... | 1990 | 2154166 |
| characterization of the h(+)-pumping f1f0 atpase of vibrio alginolyticus. | the f1f0 atpase of vibrio alginolyticus was cloned from a chromosomal lambda library. the unc operon, which contains the structural genes for the atpase, was sequenced and shown to have a gene organization of uncibefhagdc. the sequence of each subunit was compared with those of other eubacterial atpases. the v. alginolyticus unc genes exhibited greater similarity to the escherichia coli unc genes than to any of the other bacterial unc genes for which the sequence is available. the atpase was exp ... | 1990 | 2147679 |
| in vitro protein translocation into inverted membrane vesicles prepared from vibrio alginolyticus is stimulated by the electrochemical potential of na+ in the presence of escherichia coli seca. | a protein translocation system was reconstituted from inverted membrane vesicles prepared from na+ pump-possessing vibrio alginolyticus and purified escherichia coli seca. the translocation required atp and was stimulated by the functioning of the na+ pump, suggesting that the electrochemical potential of na+, but not that of h+, is important for protein translocation in vibrio. | 1990 | 2159889 |
| [isolation of vibrio alginolyticus from a patient with chronic otitis]. | | 1989 | 2490665 |
| downregulation of parathyroid hormone receptors in renal membranes from aged rats. | the mechanism of the inhibition or blunting of parathyroid hormone (pth)-stimulated na(+)-ca2+ exchange activity in renal cortical cells from aged rats was examined. the number of pth binding sites in basolateral membranes prepared from adult (6 mo) and old (24 mo) rats was quantitated by the binding of the synthetic analogue 125i-labeled [nle8,18, tyr34]bpth-(1-34) amide to the membrane. the maximum number of specific pth binding sites, bmax, was 92.7 +/- 9.3 and 36.7 +/- 6.1 fmol/mg protein, r ... | 1990 | 2168678 |
| a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india. | chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ... | 1990 | 2227713 |
| a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india. | chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ... | 1990 | 2227713 |
| intracellular na+ kinetically interferes with the rotation of the na(+)-driven flagellar motors of vibrio alginolyticus. | to understand the mechanism of na+ movement through the force-generating units of the na(+)-driven flagellar motors of vibrio alginolyticus, the effect of intracellular na+ concentration on motor rotation was investigated. control cells containing about 50 mm na+ showed good motility even at 10 mm na+ in the medium, i.e. in the absence of an inwardly directed na+ gradient. in contrast, na(+)-loaded cells containing about 400 mm na+ showed very poor motility at 500 mm na+ in the medium, i.e. even ... | 1990 | 2243095 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| evolution of antioxidant mechanisms: thiol-dependent peroxidases and thioltransferase among procaryotes. | glutathione peroxidase and glutathione s-transferase both utilize glutathione (gsh) to destroy organic hydroperoxides, and these enzymes are thought to serve an antioxidant function in mammalian cells by catalyzing the destruction of lipid hydroperoxides. only two groups of procaryotes, the purple bacteria and the cyanobacteria, produce gsh, and we show in the present work that representatives from these two groups (escherichia coli, beneckea alginolytica, rhodospirillum rubrum, chromatium vinos ... | 1989 | 2515292 |
| nucleotide sequence of the unc operon of vibrio alginolyticus. | | 1989 | 2529481 |
| differential effects of the destruction of leydig cells by administration of ethane dimethane sulphonate to postnatal rats. | ethane dimethane sulphonate (eds) is a cytotoxic drug that selectively destroys leydig cells in adult testes. this study has examined the effect of a single injection of eds on the leydig cell populations present in the testes of rats aged 5, 10, or 20 days. microscopic examination of the tissue demonstrated that the fetal leydig cell population was destroyed at all ages, but that subsequent development of the adult population of leydig cells was not affected. whilst the destruction of the fetal ... | 1989 | 2546615 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| cloning and sequencing of the saca gene: characterization of a sucrase from zymomonas mobilis. | the zymomonas mobilis gene (saca) encoding a protein with sucrase activity has been cloned in escherichia coli and its nucleotide sequence has been determined. potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to e. coli and z. mobilis consensus sequences. extracts from e. coli cells, containing the saca gene, displayed a sucrose-hydrolyzing activity. however, no transfructosylation activity (exchange reaction or levan ... | 1990 | 2254250 |
| nucleotide sequence of the vibrio alginolyticus calcium-dependent, detergent-resistant alkaline serine exoprotease a. | the nucleotide sequence of the vibrio alginolyticus alkaline serine exoprotease a (proa) gene cloned in escherichia coli was determined. the exoprotease a gene (proa) consisted of 1602 bp which encoded a protein of 534 amino acids (aa) with an mr of 55,900. the region upstream from the gene was characterized by a putative promoter consensus region (-10 -35), a ribosome-binding site and atg start codon. the proa gene encodes a typical 21-aa n-terminal signal sequence which, when fused to alkaline ... | 1989 | 2546861 |
| sgap-10c agar for the isolation and quantification of aeromonas from water. | glutamate starch penicillin (gsp) medium was used for the simultaneous isolation of pseudomonas and aeromonas. modifications to reduce the number of pseudomonas and background flora and to improve the recovery of aeromonas from water samples are described. the original medium was modified by adding glucose and ampicillin. the addition of 10 micrograms/l of c-glucose to the medium (sgap-10c) permitted better recuperation of stressed cells of aeromonads and the ampicillin reduced the numbers of ps ... | 1991 | 2016212 |
| nucleotide sequence and analysis of the vibrio alginolyticus sucrase gene (scrb). | the nucleotide sequence of a 2.119-kb dna fragment containing the vibrio alginolyticus sucrase gene (scrb) was determined. the complete sequence (484 aa residues) of the sucrase was deduced and homology was detected between the sucrase enzymes from v. alginolyticus and the gram-positive bacteria bacillus subtilis and streptococcus mutans. in escherichia coli cells the cloned v. alginolyticus sucrase is translocated to the periplasm. transposon phoa mutagenesis experiments strongly suggested that ... | 1989 | 2551785 |
| vibrios associated with red tides caused by mesodinium rubrum. | vibrios were isolated from red tides caused by mesodinium rubrum and also throughout the year in the ria de pontevedra, spain. the isolates were grouped into 14 phena by numerical toxonomy. strains associated with red tides were restricted to four phena: phena i and ii were vibrio alginolyticus, and phena iii and iv were vibrio tubiashii and vibrio anguillarum, respectively. v. anguillarum-like strains (phena v through xi) predominated throughout the year outside the red tide areas. cytotoxicity ... | 1990 | 2268167 |
| polyamine metabolism and uptake during phaseolus vulgaris lectin, pha-induced growth of rat small intestine. | kidney bean lectin, pha, stimulated the hyperplastic and hypertrophic growth of rat small intestine. this growth was preceded by a rapid accumulation of polyamines in the small intestine. however, since the lectin had little effect on in situ polyamine biosynthesis, most of the polyamines must have been of extracellular origin. to investigate the source of polyamines, both the luminal uptake and basolateral transport of polyamines by the rat small intestine were measured in vivo. luminal polyami ... | 1990 | 2262068 |
| mechanisms of sodium transport in bacteria. | in some bacteria, an na+ circuit is an important link between exergonic and endergonic membrane reactions. the physiological importance of na+ ion cycling is described in detail for three different bacteria. klebsiella pneumoniae fermenting citrate pumps na+ outwards by oxaloacetate decarboxylase and uses the na+ ion gradient thus established for citrate uptake. another possible function of the na+ gradient may be to drive the endergonic reduction of nad+ with ubiquinol as electron donor. in vib ... | 1990 | 1970650 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| nucleotide sequence and analysis of the vibrio alginolyticus scr repressor-encoding gene (scrr). | the nucleotide sequence of the vibrio alginolyticus scr repressor-encoding gene (scrr) was determined. the deduced amino acid sequence of the scr repressor was homologous with the gal, lac and cyt repressors of escherichia coli and contained a helix-turn-helix dna binding domain. although the scrr gene encoded a protein which was required for the regulation of the v. alginolyticus sucrose utilization system, a particular deletion in the scrr gene could not be complemented in trans. the lack of c ... | 1991 | 2060795 |
| [determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method]. | use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ... | 1985 | 2578835 |
| genetic transformation of vibrio parahaemolyticus, vibrio alginolyticus, and vibrio cholerae non o-1 with plasmid dna by electroporation. | an electroporation procedure for the plasmid-mediated transformation of the genus vibrio was performed, as part of an effort to develop recombinant dna techniques for genetic manipulation of the genus vibrio. vibrio parahaemolyticus, v. alginolyticus, and v. cholerae non o-1 (9 different strains) were transformed with 3 vector plasmids (pacyc184, phsg398, and pbr325). the efficiency of transformation was highly dependent on three parameters: the concentration of plasmid dna; the strength of the ... | 1990 | 2280728 |
| interactions between different strains of vibrio alginolyticus and hemolymph fractions from adult mytilus edulis. | the juvenile-bivalve pathogen vibrio alginolyticus ncmb 1339 was toxic, in vitro, to hemocytes from adult mytilus edulis. toxicity was mediated by both washed bacterial cells and culture supernates. washed cells of an environmental isolate of v. alginolyticus, strain ps-1, were 2.5 times less toxic to mytilus hemocytes, but this strain did produce a lethal extracellular factor(s) in broth culture, albeit at lower levels than v. alginolyticus ncmb 1339. hemolymph fractions from mytilus exerted a ... | 1990 | 2376663 |
| ion selectivity of the vibrio alginolyticus flagellar motor. | the marine bacterium, vibrio alginolyticus, normally requires sodium for motility. we found that lithium will substitute for sodium. in neutral ph buffers, the membrane potential and swimming speed of glycolyzing bacteria reached maximal values as sodium or lithium concentration was increased. while the maximal potentials obtained in the two cations were comparable, the maximal swimming speed was substantially lower in lithium. over a wide range of sodium concentration, the bacteria maintained a ... | 1990 | 2394685 |
| the sodium cycle. i. na+-dependent motility and modes of membrane energization in the marine alkalotolerant vibrio alginolyticus. | respiration, membrane potential generation and motility of the marine alkalotolerant vibrio alginolyticus were studied. subbacterial vesicles competent in nadh oxidation and delta psi generation were obtained. the rate of nadh oxidation by the vesicles was stimulated by na+ in a fashion specifically sensitive to submicromolar hqno (2-heptyl-4-hydroxyquinoline n-oxide) concentrations. the same amounts of hqno completely suppressed the delta psi generation. delta psi was also inhibited by cyanide, ... | 1986 | 2425848 |
| [the detection of vibrio parahaemolyticus and vibrio alginolyticus using modified culture media]. | studies have been completed for the detection of vibrio(v.) parahaemolyticus and v. alginolyticus in baltic sea fish. the author had prepared for that purpose a liquid culturing medium and a solid substrate. the percentage of positive findings is remarkable. the results so far recorded should be a good reason for further studies. | 1989 | 2619483 |
| effects of hypercortisolemia and diabetes on skeletal muscle insulin receptor function in vitro and in vivo. | activation of skeletal muscle insulin receptor tyrosine kinase in vitro and in vivo was studied in two rat models of insulin resistance: insulinopenic diabetes and hypercortisolemia. in control rats, intravenous insulin administration resulted in dose-dependent in vivo activation of the muscle insulin receptor kinase towards histone h2b. half-maximal and maximal activation were observed 5 min after injecting 0.1 and 0.5 u insulin/100 g, respectively. diabetes (7 days) induced with streptozotocin ... | 1989 | 2643342 |
| antimelanoma monoclonal antibody-ricin a chain immunoconjugate (xmmme-001-rta) plus cyclophosphamide in the treatment of metastatic malignant melanoma: results of a phase ii trial. | prior studies with the xmmme-001-rta immunoconjugate composed of an antimelanoma monoclonal antibody and ricin a chain demonstrated some antitumor activity. however, almost all patients studied developed human antimurine antibodies and antiricin antibodies. in an effort to abrogate these host anti-immunotoxin immune responses and thus enhance antitumor activity, we treated 20 patients with the immunoconjugate plus a single dose of intravenous cyclophosphamide. an overall response rate of 20% was ... | 1990 | 2395000 |
| [similarity of vibrio alginolyticus, v. cholerae and other vibrio species with respect to the structure of their flagellar apparatus and ribosomal 5s-rna]. | electron microscopic analysis of basal bodies of the flagella vibrio alginolyticus revealed a structure composed of four discs. the diameters of two discs localized in the cytoplasmic membrane appeared to be twice as little as those of the other two discs. in this respect the basal body of v. alginolyticus resembles that of v. cholerae. the 5s sequence of ribosomal rna from v. alginolyticus appeared to be similar to those of v. cholerae, v. harveyi and some other vibrios. comparison of 5s-rna se ... | 1987 | 2434145 |
| the sodium cycle: a novel type of bacterial energetics. | the progress of bioenergetic studies on the role of na+ in bacteria is reviewed. experiments performed over the past decade on several bacterial species of quite different taxonomic positions show that na+ can, under certain conditions, substitute for h+ as the coupling ion. various primary na+ pumps (delta mu na+ generators) are described, i.e., na+ -motive decarboxylases, nadh-quinone reductase, terminal oxidase, and atpase. the delta mu na+ formed is shown to be consumed by na+ driven atp-syn ... | 1989 | 2687258 |
| sodium-transport nadh-quinone reductase of a marine vibrio alginolyticus. | the respiratory chain of a marine bacterium, vibrio alginolyticus, required na+ for maximum activity, and the site of na+ -dependent activation was localized on the nadh-quinone reductase segment. the na+ -dependent nadh-quinone reductase extruded na+ as a direct result of redox reaction. it was composed of three subunits, alpha, beta, and gamma, with apparent mr of 52, 46, and 32 kda, respectively. the reduction of ubiquinone-1 to ubiquinol proceeded via ubisemiquinone radicals. the former reac ... | 1989 | 2687259 |
| comparison of substance k-like and substance p-like fibers and cells in the rat hippocampus. | substance p (sp) and substance k (sk) are structurally related peptides which are both encoded in the preprotachykinin a gene. the distribution of sp- and sk-like fibers and cell bodies in the rat hippocampus were studied by immunohistochemistry. the distribution of sk-like fibers was similar to that of sp-like fibers but there were few sk-like fibers. fibers for both peptides were prominent in the dorsal and ventral subiculum and at the junction of ca2 and ca3. sp- and sk-like cell bodies were ... | 1987 | 2446709 |
| evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the oam. | an antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ... | 1987 | 2446533 |
| biochemical and antigenic characterization of cd45 polypeptides expressed on plasma membrane and internal granules of human neutrophils. | the expression of cd45 polypeptides, a phosphotyrosine phosphatase complex specific of leukocytes, has been investigated in both resting and activated neutrophils by using anti-cd45 monoclonal antibodies (mab) which specifically recognize different polypeptide components of the cd45 molecular complex. polypeptides of 180 and 130-150 kda were equally precipitated by either a conventional cd45 mab recognizing an antigenic determinant shared by the four cd45 glycoproteins (220, 205, 190 and 180 kda ... | 1989 | 2525488 |
| a study on na+ -coupled oxidative phosphorylation: atp formation supported by artificially imposed delta pna and delta pk in vibrio alginolyticus cells. | addition of na+ to the k+-loaded vibrio alginolyticus cells, creating a 250-fold na+ gradient, is shown to induce a transient increase in the intracellular atp concentration, which is abolished by the na+/h+ antiporter, monensin. the delta pna-supported atp synthesis requires an additional driving force supplied by endogenous respiration or, alternatively, by a k+ gradient (high [k+] inside). in the former case, atp formation is resistant to the protonophorous uncoupler. dicyclohexylcarbodiimide ... | 1989 | 2473063 |
| the estimation of dietary protein intake in chronic renal failure. | forty-five adult clinic patients with chronic renal failure each supplied a 4-day weighed dietary record, a 24-h urine collection, and a nocturnal spot urine sample. total nitrogen (n) losses derived from the urines were corrected for proteinuria and non-urea nitrogen excretion. individual estimates of n intake were compared by correlation and assessing the level of agreement. daily urea n excretion derived from the spot sample correlated well with the 24-h collection p less than 0.001, but the ... | 1989 | 2515492 |
| a primary respiratory na+ pump of an anaerobic bacterium: the na+-dependent nadh:quinone oxidoreductase of klebsiella pneumoniae. | membranes of klebsiella pneumoniae, grown anaerobically on citrate, contain a nadh oxidase activity that is activated specifically by na+ or li+ ions and effectively inhibited by 2-heptyl-4-hydroxyquinoline-n-oxide (hqno). cytochromes b and d were present in the membranes, and the steady state reduction level of cytochrome b increased on nacl addition. inverted bacterial membrane vesicles accumulated na+ ions upon nadh oxidation. na+ uptake was completely inhibited by monensin and by hqno and sl ... | 1989 | 2545175 |
| [otitis caused by vibrio alginolyticus]. | | 1989 | 2525729 |
| the f1-atpase of vibrio alginolyticus. purification and n-terminal sequence of major subunits. | the f1-type atpase has been isolated from membrane preparations of marine alkalotolerant bacterium, vibrio alginolyticus. the enzyme was found to consist of two major subunits of 55 and 58 kda and at least two minor components (38 and 23 kda). amino acid sequences of n-terminal regions of the major subunits revealed close homology with those of e. coli h+-atpase and of propionigenium modestum na+-atpase. | 1989 | 2532151 |
| [the ratios and kinds of clinical bacteria isolated in taiwan's large-size hospitals]. | the prevalence of clinical bacteria, as isolated from linko chang-gung memorial hospital (2,300 beds) in the period january 1985 to december 1986 and from taipei veterans general hospital (2,300 beds) during the period january 1986 to december 1986, was analyzed with the following findings: (i) the isolation ratio of anaerobic and aerobic or facultative bactria during the period of investigation were 7.8% (5,513/70,799) and 92.2% (65,286/70,799), respectively. (ii) of the total aerobic or facult ... | 1989 | 2791722 |
| [the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. | | 1989 | 2806660 |
| concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated. | because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ... | 1989 | 2561041 |
| use of synthetic ribosome binding site for overproduction of the 5b protein of insertion sequence is5. | insertion sequence is5 is a bacterial transposable element which contains three open reading frames designated 5a, 5b and 5c. although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site, we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site. beta-galac ... | 1989 | 2538799 |
| effect of cyclosporin a immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig. | female guinea pigs pretreated for 2 days with cyclosporin a (csa), were then inoculated intranasally (in) or intraperitoneally (ip) with a lymphotropic herpesvirus (gphlv) and followed by 4 additional daily doses of csa. immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. while in-inoculated csa-treated animals expressed higher virus infectivity titer in th ... | 1987 | 2832345 |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| evaluation of media for monitoring fecal streptococci in seawater. | the selectivity of kf streptococcus agar (kf) for monitoring fecal streptococci (fs) in seawater was examined in 234 samples of mediterranean water and compared with the selectivity of m-enterococcus agar (m-ent) for 124 samples and with bile-esculin-azide agar (bea) for 17 samples. kf was found to be unsuitable for marine water because vibrio alginolyticus and other gram-negative bacilli indigenous to this environment grew well on it and produced red colonies identical to those of fs. in 26% of ... | 1989 | 2782876 |
| inadequate blockade by hexamethonium of the baroreceptor heart rate response in anesthetized and conscious rats. | the ability of hexamethonium (hex) alone or in combination with atropine methylbromide (amb) to block the heart rate (hr) response to baroreceptor activation by phenylephrine (pe) or angiotensin ii (aii)-evoked increments in blood pressure was evaluated in rats. a highly significant negative correlation existed under control conditions between the changes in map and hr. hex (10-20 mg/kg) did not influence the baroreceptor hr response of anesthetized and conscious rats, suggesting anesthesia was ... | 1989 | 2567154 |
| the na(+)-motive respiratory chain of marine bacteria. | the respiratory chain of the marine bacterium vibrio alginolyticus pumps out na+ at the nadh:quinone oxidoreductase segment. the respiratory na+ pump plays an important role in the bioenergetics of this bacterium by generating a sodium-motive force as a direct result of respiration in alkaline na(+)-rich environments. | 1985 | 2856376 |