structural gene and complete amino acid sequence of vibrio alginolyticus collagenase.the dna encoding the collagenase of vibrio alginolyticus was cloned, and its complete nucleotide sequence was determined. when the cloned gene was ligated to puc18, the escherichia coli expression vector, bacteria carrying the gene exhibited both collagenase antigen and collagenase activity. the open reading frame from the atg initiation codon was 2442 bp in length for the collagenase structural gene. the amino acid sequence, deduced from the nucleotide sequence, revealed that the mature collage ...19921311172
roles of k+ and na+ in ph homeostasis and growth of the marine bacterium vibrio alginolyticus.the marine bacterium vibrio alginolyticus, containing 470 mm-k+ and 70 mm-na+ inside its cells, was able to regulate the cytoplasmic ph (ph(in)) in the narrow range 7.6-7.8 over the external ph (ph(out)) range 6.0-9.0 in the presence of 400 mm-na+ and 10 mm-k+. in the absence of external k+, however, phin was regulated only at alkaline ph(out) values above 7.6. when the cells were incubated in the presence of unusually high k+ (400 mm) and 4 mm na+, the ph(in) was regulated only at acidic ph(out ...19921326594
catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus.cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ...19921328162
fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection.thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ...19921347287
[h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors].among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ...19921347325
towards a phylogeny of the genus vibrio based on 16s rrna sequences.the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ...19921371064
variations in rrna content of marine vibrio spp. during starvation-survival and recovery.the degree and temporal context of variations in ribosome content during nutrient starvation of two copiotrophic marine bacteria, vibrio alginolyticus and vibrio furnissii, have been examined. the organisms were starved either by nutritional shift-down or by consumption of limiting nutrients resulting from growth into stationary phase. measurements of the amount of hybridization to 16s rrna-specific probes revealed that the cells retained between 10 and 26% of their original rrna content after 1 ...19921371659
expression of bacillus amyloliquefaciens amylase and vibrio alginolyticus protease a fusion genes.previously we reported [deane, s. m., maharaj, r., robb, f. t. & woods, d. r. (1987) journal of general microbiology 133, 2295-2302] that the production of a vibrio alginolyticus sds-resistant alkaline serine protease (pro a) cloned in escherichia coli was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active pro a. replacement of the v. alginolyticus promoter region by the alpha-amylase promoter region from bacillus amyloliquefaciens resulted in th ...19921373436
mapping of antigenic sites to monoclonal antibodies on the primary structure of the f1-atpase beta subunit from escherichia coli: concealed amino-terminal region of the subunit in the analyze relationships between the ternary and primary structures of the beta subunit of escherichia coli f1 atpase, we prepared two monoclonal antibodies beta 12 and beta 31 against the beta peptide. these antibodies bind to the beta subunit but do not bind to the f1 atpase, resulting in no inhibition of the atpase activities. several different portions of the beta subunit peptide were prepared by constructing expression plasmids carrying the corresponding dna segment of the beta subunit gene ...19921373594
incidence of toxigenic vibrios in foods available in taiwan.a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ...19921399913
characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins.irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ...19921406279
prediction of transmembrane topology of f0 proteins from escherichia coli f1f0 atp synthase using variational and hydrophobic moment analyses.the a subunit, a membrane protein from the e. coli f1f0 atp synthase has been examined by fourier analysis of hydrophobicity and of amino-acid residue variation. the amino-acid sequences of homologous subunits from vibrio alginolyticus, saccharomyces cerevisiae, neurospora crassa, aspergillus nidulans, schizosaccharomyces pombe and candida parapsilosis were used in the variability analysis. by fourier analysis of sequence variation, two transmembrane helices are predicted to have one face in con ...19921445940
[vibrio alginolyticus in clinical specimens]. 19921450271
[membrane atpase of vibrio alginolyticus. ion transport activity and homology with f0f1-atpase from e. coli].f0f1-atpase has been isolated from the marine alkali-resistant bacterium vibrio alginolyticus. the enzyme subunits cross-reacted with antibodies against subunits alpha, beta, gamma, epsilon, and b of e. coli atpase. the purified atpase was reconstituted into liposomes effecting an atp-dependent uptake of h+. proton transport was inhibited by the atpase blockers dccd, triphenyltin, and venturicidin. na+ ions had no effect on atp-dependent proton transport. no atp-dependent transport of na+ was de ...19921457596
amiloride at ph 7.0 inhibits the na(+)-driven flagellar motors of vibrio alginolyticus but allows cell growth.amiloride, a specific inhibitor for the na(+)-driven flagellar motors of alkalophilic bacillus, is known to inhibit secondarily the growth of alkalophiles. the motility of a marine vibrio, v. alginolyticus, was almost completely inhibited by 2 mm amiloride either at ph 7.0 or 8.5. we found that this concentration of amiloride inhibited the cell growth completely at ph 8.5 but only slightly at ph 7.0. kinetic analysis of the inhibition of motility by amiloride at ph 7.0 showed that the inhibition ...19921459240
ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride.intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ...19921479187
vibrio alginolyticus ("achromobacter") collagenase: biosynthesis, function and application.bacterial collagenase from aerobic non-pathogenic vibrio alginolyticus chemovar iophagus ("achromobacter" collagenase, ec is an inducible extracellular metallo-proteinase. production of vibrio collagenase is induced specifically by collagen or by its macromolecular fragments. on the cell surface is expressed a specific receptor recognizing collagen structure. the study of natural inducers led to synthetic peptides with inducing properties. vibrio collagenase cleaves collagen helical c ...19921480012
development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans, fish, and shellfish.monoclonal antibodies (mabs) against vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. the pathogens included vibrio alginolyticus, v. anguillarum, v. carchariae, v. cholerae, v. damsela, v. furnissii, v. harveyi, v. ordalii, v. parahaemolyticus, and v. vulnificus. three types of mabs were selected. the first important group included mabs that reacted with only a single species. a second group comprised a number of mabs that reacted w ...19921482190
purification and characterization of l-2,4-diaminobutyrate decarboxylase from acinetobacter calcoaceticus.acinetobacter calcoaceticus atcc 23055 produces a large amount of 1,3-diaminopropane under normal growth conditions. the enzyme responsible, l-2,4-diaminobutyrate (daba) decarboxylase (ec 4.1.1.-), was purified to electrophoretic homogeneity from this bacterium. the native enzyme had an m(r) of approximately 108,000, with a pi of 5.0, and was a dimer composed of identical or nearly identical subunits with apparent m(r) 53,000. the enzyme showed hyperbolic kinetics with a km of 1.59 mm for daba a ...19921512577
interleukin-1 receptor antagonist protein inhibits interleukin-8 expression in lipopolysaccharide-stimulated human whole blood.interleukin-8 (il-8) is a neutrophil and lymphocyte chemoattractant and activator that may play an important role in mediating events at sites of inflammation. il-8 is produced by many cell types in response to a variety of inducers, including interleukin-1 (il-1). studies were conducted to address the question of whether an inhibitor of il-1 action, il-1 receptor antagonist protein (irap), would suppress il-8 production. lipopolysaccharide (lps)-stimulated human whole blood was used as an ex vi ...19921533750
properties of respiratory chain-linked na(+)-independent nadh-quinone reductase in a marine vibrio alginolyticus.the respiratory chain of a marine vibrio alginolyticus contains two types of nadh-quinone reductase (nqr): one is an na(+)-dependent nqr functioning as an na+ pump (nqr-1) and the other is an na(+)-independent nqr (nqr-2). nqr-2 was purified about 55-fold from the membrane of mutant nap-1 which is devoid of nqr-1, and its properties were compared with those of nqr-1. in contrast to nqr-1, the purified nqr-2 does not require any salts for activity and is not inhibited by up to 0.4 m salts. the op ...19921543699
[changes in centrally released arginine vasopressin by stimulation of the medullary reticular formation].it has been reported that after 40 minutes of stimulation of the medullary reticular formation (morf), widespread significant increase by 1.4% to 2.8% in brain water content occurs in white matter of the injured hemisphere. recent studies indicate that centrally released arginine vasopressin (avp) influences water permeability of the brain in both normal and pathological conditions. the present study was carried out to clarify the effect of electrical stimulation of morf on centrally released av ...19921562384
the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes.chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ...19921594593
characteristic differences in the mode of quinone reduction and stability between energy-coupled and -uncoupled nadh-quinone reductases from bacterial respiratory chain.bacterial respiratory chain has two types of nadh-quinone reductase (nqr): one is energy-coupled (type-1) and the other had no energy-transducing capacity, that is, energy-uncoupled (type-2). each of the nadh-reacting flavoprotein subunits of nqr-1 from escherichia coli and the marine vibrio alginolyticus reduced quinone to semiquinone radicals by the one-electron transfer pathway and was very sensitive to preincubation with nadh. on the other hand, the nqr-2 from these bacteria reduced quinone ...19921628743
f0f1-atpase from vibrio alginolyticus. subunit composition and proton pumping f0f1-atpase was isolated from the membranes of the marine bacterium vibrio alginolyticus. homology between the subunits of the f0-complexes from e. coli and v. alginolyticus was found using antibodies against subunits a, b and c of the e. coli f0f1-atpase. the f0f1-complex from v. alginolyticus was reconstituted into proteoliposomes, which were competent in atp-dependent proton uptake. this process was inhibited by triphenyltin, dccd, and venturicidin. na+ did not affect proton translocation.19911647986
regulation of the mouse mammary tumor virus (mmtv) binding site in cultured mammary tissue.the mouse mammary tumor virus (mmtv) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. the hormonal regulation of this protein was studied both in vitro and in vivo. in mammary gland explant culture, levels of the mmtv binding protein were maintained only when prolactin was present in the medium. the further addition of progesterone induced levels to 164% of controls, while estradiol was without effect. these results were reproduce ...19911665450
na(+)-coupled alternative to h(+)-coupled primary transport systems in bacteria.protons are the most common coupling ions in bacterial energy conversions. however, while many organisms, such as the alkaliphilic bacilli, employ h(+)-bioenergetics for electron transport phosphorylation, they use na+ as the coupling ion for transport and flagellar movement. the na+ gradient required for these bioenergetic functions is established by the secondary na+/h+ antiporter. in contrast, vibrio alginolyticus and methanogenic bacteria have primary pumps for both h+ and na+. they use the ...19911665692
vibrio alginolyticus in the u.k. 19911753130
the sodiummotive nadh-ubiquinone oxidoreductase of vibrio alginolyticus. 19911783108
isolation of vibrio alginolyticus from blood cultures in a leukaemic patient after consumption of oysters. 19911794376
two generalized transducing phages in vibrio parahaemolyticus and vibrio alginolyticus.two bacteriophages named phi vp253 and phi vp143 isolated after ultraviolet induction from lysogenic strains of vibrio parahaemolyticus have been shown to be generalized transducing phages. so far, seven different auxotrophic markers of a v. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2 x 10(-7) to 7.5 x 10(-5) per infected cell at the m.o.i. of approximately 1.0. the phage phi vp143, but not phi vp253, lysed 20 of the 28 strains of v. alginolyticus and the occu ...19911808461
molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria.sucrose-positive derivatives of escherichia coli k-12, containing the plasmid pur400, and of klebsiella pneumoniae hydrolyse intracellular sucrose 6-phosphate by means of an invertase into d-glucose 6-phosphate and free d-fructose. the latter is phosphorylated by an atp-dependent fructokinase (gene scrk of an scr regulon) to d-fructose 6-phosphate. the lack of scrk does not cause any visible phenotype in wild-type strains of both organisms. using genes and enzymes normally involved in d-arabinit ...19911809835
[identification of species of microorganisms of the genus vibrio].during 1988, a study was made on 61 microorganisms, genus vibrio, which were received at the national reference laboratory for acute diarrheic diseases. pedro kouri institute of tropical medicine. of them, 46 were from children with acute diarrheic disease and 15 were isolated from the environment. by means of biochemical tests. 61 vibrio cholerae no. 01. 9 vibrio parahaemolyticus and 1 vibrio alginolyticus were identified. emphasis is placed upon the importance of keeping a systematic surveilla ...19911812527
[vibrio alginolyticus and swimmer's otitis externa. 2 cases and review of the literature].we describe two patients with acute diffuse external otitis (swimmer's otitis) acquired in the mediterranean shore, with vibrio alginolyticus recovered from ear fluid. we describe the biochemical profile and sensitivity pattern (mic) of both strains, comparing it to previously published data. a literature review was also performed, in which we found evidence for increasing concern of v. alginolyticus as an human pathogen. also there is a need for considering halophilic vibrio as potential pathog ...19911822155
the effect of f0 inhibitors on the vibrio alginolyticus membrane atpase.the inhibition of membrane atpase from the marine alkalotolerant bacterium vibrio alginolyticus by dccd, triphenyltin and venturicidin was studied. dccd proved to be an irreversible inhibitor, while venturicidin and triphenyltin produced a reversible inhibitory effect. the dccd-binding proteolipid was identified in the membrane preparations. the effect of the inhibitors on atpase activity and atp-dependent na(+)-transport in v. alginolyticus subcellular vesicles is discussed.19911826482
structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6.we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ...19911828805
complexity and sequence identification of 24 rat v beta genes.twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ...19911828824
fluorescence quenching studies on the characterization of energy generated at the nadh:quinone oxidoreductase and quinol oxidase segments of marine bacteria.generation of membrane potential (inside-positive) and delta ph (inside-acidic) at two kinds of nadh:quinone oxidoreductase segments, the na(+)-motive segment and another segment, of vibrio alginolyticus was examined by monitoring the quenching of fluorescence of oxonol v and that of quinacrine, respectively, with inside-out membrane vesicles. transient generation of membrane potential at the segment occurred when ubiquinone-1 was added in the presence of kcn and nadh. the membrane potential was ...19911907969
effect of systemic inhibition of prostaglandin synthesis on muscle protein balance after trauma in the rat.anaesthetized rats were subjected to a single impact trauma to the medial aspect of the right hindlimb (gastrocnemius muscle), and were compared with sham-treated controls. for 3 days after injury, muscles of the traumatized limb showed a marked catabolic response. muscle protein repletion commenced after day 3, however, this process was not complete until 21 days after injury. muscles of the uninjured limb of the traumatized rats also showed a distinct catabolic response, compared with rats tha ...19911913329
[creatinine clearance as the foundation for clinical determination of kidney function in swine].comparative examinations of renal function using inulin- and endogenous creatinine-clearance tests in 5 pigs showed nearly identical values of glomerular filtration rate (gfr). based on this relation 79 healthy pigs, weighting 2 kg to 230 kg were investigated using the creatinine-clearance test for determination of normal values of gfr and urine flow rate (vu) as well as renal excretion (e), renal clearance (clr) and fractional excretion (fe) of urea, sodium, potassium, calcium, phosphorus, gluc ...19911948984
detection of antigens in urine of patients with acute falciparum and vivax malaria infections.using an antigen-capture, dot-blot assay, antigens were detected in the urine of 50 patients infected with plasmodium falciparum. antigens were also detected in 12/15 patients who had no detectable parasitemias 1-2 weeks after chemotherapy. by western blotting and immunoprecipitation, four predominant antigens were identified with the following molecular masses (mr) and isoelectric points (pi): antigen 1, 200 kda, pi 6.4-6.27; antigen 2, 180 kda, pi 5.2-4.8; antigen 3, 150 kda, pi 5.5; antigen 4 ...19911951853
purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine in vibrio alginolyticus.carboxynorspermidine synthase, mediates the nicotinamide-nucleotide-linked reduction of the schiff base h2n(ch2)3n = chch2ch(nh2)cooh. this is formed from l-aspartic beta-semialdehyde (asa) and 1,3-diaminopropane (dap) and is reduced to carboxynorspermidine [h2n(ch2)3nh(ch2)2ch(nh2)cooh], an intermediate in the novel pathway for norspermidine (nspd) biosynthesis. the enzyme was purified to apparent homogeneity from vibrio alginolyticus and characterized. the overall purification was about 1800-f ...19911955861
molecular cloning and partial dna sequencing of the collagenase gene of vibrio alginolyticus.dna fragments vibrio alginolyticus chemovar iophagus, at least 7 kb in length, were ligated to escherichia coli expression vectors. three clones of escherichia coli hb101 (plco-1, plco-2, plco-3) were obtained by the colony immunoblotting method using anti-collagenase antibody. in escherichia coli, all these genes produced collagenase antigens which were detected with western blotting. the amino acid sequence of chemically purified collagenase fragments was also analyzed. an approximately 2.5 kb ...19901965912
mechanisms of sodium transport in some bacteria, an na+ circuit is an important link between exergonic and endergonic membrane reactions. the physiological importance of na+ ion cycling is described in detail for three different bacteria. klebsiella pneumoniae fermenting citrate pumps na+ outwards by oxaloacetate decarboxylase and uses the na+ ion gradient thus established for citrate uptake. another possible function of the na+ gradient may be to drive the endergonic reduction of nad+ with ubiquinol as electron donor. in vib ...19901970650
sgap-10c agar for the isolation and quantification of aeromonas from water.glutamate starch penicillin (gsp) medium was used for the simultaneous isolation of pseudomonas and aeromonas. modifications to reduce the number of pseudomonas and background flora and to improve the recovery of aeromonas from water samples are described. the original medium was modified by adding glucose and ampicillin. the addition of 10 micrograms/l of c-glucose to the medium (sgap-10c) permitted better recuperation of stressed cells of aeromonads and the ampicillin reduced the numbers of ps ...19912016212
nucleotide sequence and analysis of the vibrio alginolyticus scr repressor-encoding gene (scrr).the nucleotide sequence of the vibrio alginolyticus scr repressor-encoding gene (scrr) was determined. the deduced amino acid sequence of the scr repressor was homologous with the gal, lac and cyt repressors of escherichia coli and contained a helix-turn-helix dna binding domain. although the scrr gene encoded a protein which was required for the regulation of the v. alginolyticus sucrose utilization system, a particular deletion in the scrr gene could not be complemented in trans. the lack of c ...19912060795
yolk degradation in tick eggs: ii. evidence that cathepsin l-like proteinase is stored as a latent, acid-activable proenzyme.cathepsin l-like proteinase found in the eggs of the tick ornithodoros moubata is latent during embryogenesis, but can be activated by acid treatment. in crude extracts as well as in partially purified fractions, activation requires reducing conditions and is inhibited by leupeptin, which indicates that it is mediated by a thiol proteinase, probably by the cathepsin l itself. latency disappears in vivo at the time of the acute phase of yolk digestion, which takes place during late embryonic deve ...19902134179
sequence of subunit c of the sodium ion translocating adenosine triphosphate synthase of propionigenium modestum.the 30 n-terminal amino acid residues of the purified atpase c subunit of propionigenium modestum have been determined. an oligonucleotide mixture was derived from this sequence and used as probe for cloning the corresponding gene in escherichia coli. the nucleotide sequence of the gene has been determined and compared with those of atpase c subunits from other bacteria and chloroplasts. peculiar sequence similarities are found only at the c-terminus between the c subunits of the atpases from p. ...19902146118
characterization of the h(+)-pumping f1f0 atpase of vibrio alginolyticus.the f1f0 atpase of vibrio alginolyticus was cloned from a chromosomal lambda library. the unc operon, which contains the structural genes for the atpase, was sequenced and shown to have a gene organization of uncibefhagdc. the sequence of each subunit was compared with those of other eubacterial atpases. the v. alginolyticus unc genes exhibited greater similarity to the escherichia coli unc genes than to any of the other bacterial unc genes for which the sequence is available. the atpase was exp ...19902147679
effects of respiratory activity on starvation survival of marine vibrios.the marine bacterium vibrio fluvialis nctc11328 responded to nutrient depletion by a reduction in cell volume, and this was prevented by conditions that eliminated respiration as a source of energy. addition of the protonophore, cccp, removal of oxygen and introduction of mutations leading to defects of the respiratory chain prevented size reduction during periods of nutrient limitation. further, survival of the wild-type strain during starvation was reduced under anaerobic conditions and surviv ...19902154166
in vitro protein translocation into inverted membrane vesicles prepared from vibrio alginolyticus is stimulated by the electrochemical potential of na+ in the presence of escherichia coli seca.a protein translocation system was reconstituted from inverted membrane vesicles prepared from na+ pump-possessing vibrio alginolyticus and purified escherichia coli seca. the translocation required atp and was stimulated by the functioning of the na+ pump, suggesting that the electrochemical potential of na+, but not that of h+, is important for protein translocation in vibrio.19902159889
[isolation of vibrio alginolyticus from a leg ulcer].an organism presenting the characteristics of vibrio alginolyticus was isolated from a cutaneous leg ulcer in a 67-year-old woman. the origin of this halophilic bacterium remains unknown. the clinical observation and the bacteriological findings are presented and commented.19902161606
downregulation of parathyroid hormone receptors in renal membranes from aged rats.the mechanism of the inhibition or blunting of parathyroid hormone (pth)-stimulated na(+)-ca2+ exchange activity in renal cortical cells from aged rats was examined. the number of pth binding sites in basolateral membranes prepared from adult (6 mo) and old (24 mo) rats was quantitated by the binding of the synthetic analogue 125i-labeled [nle8,18, tyr34]bpth-(1-34) amide to the membrane. the maximum number of specific pth binding sites, bmax, was 92.7 +/- 9.3 and 36.7 +/- 6.1 fmol/mg protein, r ...19902168678
longitudinal study of epstein-barr virus genotypes associated with infectious mononucleosis patients and healthy carriers. 19902169503
nucleotide sequence and analysis of the vibrio alginolyticus sucrose uptake-encoding region.the nucleotide sequence of the vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scra and scrk. the scra gene encodes an enzyme iisucrose (eiiscr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrk gene encodes a fructokinase. the deduced amino acid (aa) sequence for the v. alginolyticus eiiscr protein was homologous with the eiiscr proteins from streptococcus mutans, salmonella typhimurium (pur400 system) and bacillus s ...19902174811
radioimmunotargeting of human tumour cells in immunocompetent animals.a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ...19902223574
radioimmunotargeting of human tumour cells in immunocompetent animals.a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ...19902223574
a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india.chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ...19902227713
a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india.chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ...19902227713
intracellular na+ kinetically interferes with the rotation of the na(+)-driven flagellar motors of vibrio understand the mechanism of na+ movement through the force-generating units of the na(+)-driven flagellar motors of vibrio alginolyticus, the effect of intracellular na+ concentration on motor rotation was investigated. control cells containing about 50 mm na+ showed good motility even at 10 mm na+ in the medium, i.e. in the absence of an inwardly directed na+ gradient. in contrast, na(+)-loaded cells containing about 400 mm na+ showed very poor motility at 500 mm na+ in the medium, i.e. even ...19902243095
neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats.intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ...19902245800
neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats.intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ...19902245800
cloning and sequencing of the saca gene: characterization of a sucrase from zymomonas mobilis.the zymomonas mobilis gene (saca) encoding a protein with sucrase activity has been cloned in escherichia coli and its nucleotide sequence has been determined. potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to e. coli and z. mobilis consensus sequences. extracts from e. coli cells, containing the saca gene, displayed a sucrose-hydrolyzing activity. however, no transfructosylation activity (exchange reaction or levan ...19902254250
polyamine metabolism and uptake during phaseolus vulgaris lectin, pha-induced growth of rat small intestine.kidney bean lectin, pha, stimulated the hyperplastic and hypertrophic growth of rat small intestine. this growth was preceded by a rapid accumulation of polyamines in the small intestine. however, since the lectin had little effect on in situ polyamine biosynthesis, most of the polyamines must have been of extracellular origin. to investigate the source of polyamines, both the luminal uptake and basolateral transport of polyamines by the rat small intestine were measured in vivo. luminal polyami ...19902262068
vibrios associated with red tides caused by mesodinium rubrum.vibrios were isolated from red tides caused by mesodinium rubrum and also throughout the year in the ria de pontevedra, spain. the isolates were grouped into 14 phena by numerical toxonomy. strains associated with red tides were restricted to four phena: phena i and ii were vibrio alginolyticus, and phena iii and iv were vibrio tubiashii and vibrio anguillarum, respectively. v. anguillarum-like strains (phena v through xi) predominated throughout the year outside the red tide areas. cytotoxicity ...19902268167
genetic transformation of vibrio parahaemolyticus, vibrio alginolyticus, and vibrio cholerae non o-1 with plasmid dna by electroporation procedure for the plasmid-mediated transformation of the genus vibrio was performed, as part of an effort to develop recombinant dna techniques for genetic manipulation of the genus vibrio. vibrio parahaemolyticus, v. alginolyticus, and v. cholerae non o-1 (9 different strains) were transformed with 3 vector plasmids (pacyc184, phsg398, and pbr325). the efficiency of transformation was highly dependent on three parameters: the concentration of plasmid dna; the strength of the ...19902280728
generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein.the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ...19902292586
copper-induced production of copper-binding supernatant proteins by the marine bacterium vibrio alginolyticus.growth of the marine bacterium vibrio alginolyticus is temporarily inhibited by micromolar levels of copper. during the copper-induced lag phase, supernatant compounds which complex and detoxify copper are produced. in this study two copper-inducible supernatant proteins having molecular masses of ca. 21 and 19 kilodaltons (cubp1 and cubp2) were identified; these proteins were, respectively, 25 and 46 times amplified in supernatants of copper-challenged cultures compared with controls. experimen ...19902339887
improved fluorogenic assay for rapid detection of vibrio parahaemolyticus in improved fluorogenic assay for the rapid detection of vibrio parahaemolyticus was developed. in the improved assay, the enrichment of v. parahaemolyticus was carried out in arabinose-glucuronate medium (0.5% arabinose, 0.25% glucuronate, 0.1% polypeptone, 0.1% yeast extract, 0.1% ammonium sulfate, 2% nacl, 2 micrograms of polymyxin b sulfate per ml, ph 8.5) at 37 degrees c. after the cultivation, the trypsinlike activity of the bacteria was measured by fluorescence with the fluorogenic substr ...19902339897
number and types of hemocytes in sunetta scripta and villorita cyprinoides var. cochinensis (bivalvia), and leukocytosis subsequent to bacterial challenge.the number and types of hemocytes in four size groups of the clam species sunetta scripta and villorita cyprinoides var. cochinensis, and leukocytosis in the 38- to 40-mm-size-group clams of both species subsequent to challenge with vibrio alginolyticus at a concentration of 1 x 10(8) cells/0.02 ml of sterile 2% saline was investigated. in s. scripta, the mean total hemocyte count in the 42- to 44-mm size group was significantly lower than that of the three other size groups but there was no sig ...19902351842
interactions between different strains of vibrio alginolyticus and hemolymph fractions from adult mytilus edulis.the juvenile-bivalve pathogen vibrio alginolyticus ncmb 1339 was toxic, in vitro, to hemocytes from adult mytilus edulis. toxicity was mediated by both washed bacterial cells and culture supernates. washed cells of an environmental isolate of v. alginolyticus, strain ps-1, were 2.5 times less toxic to mytilus hemocytes, but this strain did produce a lethal extracellular factor(s) in broth culture, albeit at lower levels than v. alginolyticus ncmb 1339. hemolymph fractions from mytilus exerted a ...19902376663
ion selectivity of the vibrio alginolyticus flagellar motor.the marine bacterium, vibrio alginolyticus, normally requires sodium for motility. we found that lithium will substitute for sodium. in neutral ph buffers, the membrane potential and swimming speed of glycolyzing bacteria reached maximal values as sodium or lithium concentration was increased. while the maximal potentials obtained in the two cations were comparable, the maximal swimming speed was substantially lower in lithium. over a wide range of sodium concentration, the bacteria maintained a ...19902394685
antimelanoma monoclonal antibody-ricin a chain immunoconjugate (xmmme-001-rta) plus cyclophosphamide in the treatment of metastatic malignant melanoma: results of a phase ii trial.prior studies with the xmmme-001-rta immunoconjugate composed of an antimelanoma monoclonal antibody and ricin a chain demonstrated some antitumor activity. however, almost all patients studied developed human antimurine antibodies and antiricin antibodies. in an effort to abrogate these host anti-immunotoxin immune responses and thus enhance antitumor activity, we treated 20 patients with the immunoconjugate plus a single dose of intravenous cyclophosphamide. an overall response rate of 20% was ...19902395000
the sodium cycle. i. na+-dependent motility and modes of membrane energization in the marine alkalotolerant vibrio alginolyticus.respiration, membrane potential generation and motility of the marine alkalotolerant vibrio alginolyticus were studied. subbacterial vesicles competent in nadh oxidation and delta psi generation were obtained. the rate of nadh oxidation by the vesicles was stimulated by na+ in a fashion specifically sensitive to submicromolar hqno (2-heptyl-4-hydroxyquinoline n-oxide) concentrations. the same amounts of hqno completely suppressed the delta psi generation. delta psi was also inhibited by cyanide, ...19862425848
[similarity of vibrio alginolyticus, v. cholerae and other vibrio species with respect to the structure of their flagellar apparatus and ribosomal 5s-rna].electron microscopic analysis of basal bodies of the flagella vibrio alginolyticus revealed a structure composed of four discs. the diameters of two discs localized in the cytoplasmic membrane appeared to be twice as little as those of the other two discs. in this respect the basal body of v. alginolyticus resembles that of v. cholerae. the 5s sequence of ribosomal rna from v. alginolyticus appeared to be similar to those of v. cholerae, v. harveyi and some other vibrios. comparison of 5s-rna se ...19872434145
evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ...19872446533
comparison of substance k-like and substance p-like fibers and cells in the rat hippocampus.substance p (sp) and substance k (sk) are structurally related peptides which are both encoded in the preprotachykinin a gene. the distribution of sp- and sk-like fibers and cell bodies in the rat hippocampus were studied by immunohistochemistry. the distribution of sk-like fibers was similar to that of sp-like fibers but there were few sk-like fibers. fibers for both peptides were prominent in the dorsal and ventral subiculum and at the junction of ca2 and ca3. sp- and sk-like cell bodies were ...19872446709
a study on na+ -coupled oxidative phosphorylation: atp formation supported by artificially imposed delta pna and delta pk in vibrio alginolyticus cells.addition of na+ to the k+-loaded vibrio alginolyticus cells, creating a 250-fold na+ gradient, is shown to induce a transient increase in the intracellular atp concentration, which is abolished by the na+/h+ antiporter, monensin. the delta pna-supported atp synthesis requires an additional driving force supplied by endogenous respiration or, alternatively, by a k+ gradient (high [k+] inside). in the former case, atp formation is resistant to the protonophorous uncoupler. dicyclohexylcarbodiimide ...19892473063
[isolation of vibrio alginolyticus from a patient with chronic otitis]. 19892490665
evolution of antioxidant mechanisms: thiol-dependent peroxidases and thioltransferase among procaryotes.glutathione peroxidase and glutathione s-transferase both utilize glutathione (gsh) to destroy organic hydroperoxides, and these enzymes are thought to serve an antioxidant function in mammalian cells by catalyzing the destruction of lipid hydroperoxides. only two groups of procaryotes, the purple bacteria and the cyanobacteria, produce gsh, and we show in the present work that representatives from these two groups (escherichia coli, beneckea alginolytica, rhodospirillum rubrum, chromatium vinos ...19892515292
the estimation of dietary protein intake in chronic renal failure.forty-five adult clinic patients with chronic renal failure each supplied a 4-day weighed dietary record, a 24-h urine collection, and a nocturnal spot urine sample. total nitrogen (n) losses derived from the urines were corrected for proteinuria and non-urea nitrogen excretion. individual estimates of n intake were compared by correlation and assessing the level of agreement. daily urea n excretion derived from the spot sample correlated well with the 24-h collection p less than 0.001, but the ...19892515492
biochemical and antigenic characterization of cd45 polypeptides expressed on plasma membrane and internal granules of human neutrophils.the expression of cd45 polypeptides, a phosphotyrosine phosphatase complex specific of leukocytes, has been investigated in both resting and activated neutrophils by using anti-cd45 monoclonal antibodies (mab) which specifically recognize different polypeptide components of the cd45 molecular complex. polypeptides of 180 and 130-150 kda were equally precipitated by either a conventional cd45 mab recognizing an antigenic determinant shared by the four cd45 glycoproteins (220, 205, 190 and 180 kda ...19892525488
[otitis caused by vibrio alginolyticus]. 19892525729
nucleotide sequence of the unc operon of vibrio alginolyticus. 19892529481
the f1-atpase of vibrio alginolyticus. purification and n-terminal sequence of major subunits.the f1-type atpase has been isolated from membrane preparations of marine alkalotolerant bacterium, vibrio alginolyticus. the enzyme was found to consist of two major subunits of 55 and 58 kda and at least two minor components (38 and 23 kda). amino acid sequences of n-terminal regions of the major subunits revealed close homology with those of e. coli h+-atpase and of propionigenium modestum na+-atpase.19892532151
use of synthetic ribosome binding site for overproduction of the 5b protein of insertion sequence is5.insertion sequence is5 is a bacterial transposable element which contains three open reading frames designated 5a, 5b and 5c. although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site, we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site. beta-galac ...19892538799
a primary respiratory na+ pump of an anaerobic bacterium: the na+-dependent nadh:quinone oxidoreductase of klebsiella pneumoniae.membranes of klebsiella pneumoniae, grown anaerobically on citrate, contain a nadh oxidase activity that is activated specifically by na+ or li+ ions and effectively inhibited by 2-heptyl-4-hydroxyquinoline-n-oxide (hqno). cytochromes b and d were present in the membranes, and the steady state reduction level of cytochrome b increased on nacl addition. inverted bacterial membrane vesicles accumulated na+ ions upon nadh oxidation. na+ uptake was completely inhibited by monensin and by hqno and sl ...19892545175
differential effects of the destruction of leydig cells by administration of ethane dimethane sulphonate to postnatal rats.ethane dimethane sulphonate (eds) is a cytotoxic drug that selectively destroys leydig cells in adult testes. this study has examined the effect of a single injection of eds on the leydig cell populations present in the testes of rats aged 5, 10, or 20 days. microscopic examination of the tissue demonstrated that the fetal leydig cell population was destroyed at all ages, but that subsequent development of the adult population of leydig cells was not affected. whilst the destruction of the fetal ...19892546615
nucleotide sequence of the vibrio alginolyticus calcium-dependent, detergent-resistant alkaline serine exoprotease a.the nucleotide sequence of the vibrio alginolyticus alkaline serine exoprotease a (proa) gene cloned in escherichia coli was determined. the exoprotease a gene (proa) consisted of 1602 bp which encoded a protein of 534 amino acids (aa) with an mr of 55,900. the region upstream from the gene was characterized by a putative promoter consensus region (-10 -35), a ribosome-binding site and atg start codon. the proa gene encodes a typical 21-aa n-terminal signal sequence which, when fused to alkaline ...19892546861
nucleotide sequence and analysis of the vibrio alginolyticus sucrase gene (scrb).the nucleotide sequence of a 2.119-kb dna fragment containing the vibrio alginolyticus sucrase gene (scrb) was determined. the complete sequence (484 aa residues) of the sucrase was deduced and homology was detected between the sucrase enzymes from v. alginolyticus and the gram-positive bacteria bacillus subtilis and streptococcus mutans. in escherichia coli cells the cloned v. alginolyticus sucrase is translocated to the periplasm. transposon phoa mutagenesis experiments strongly suggested that ...19892551785
enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus.we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ...19892556341
concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated.because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ...19892561041
inadequate blockade by hexamethonium of the baroreceptor heart rate response in anesthetized and conscious rats.the ability of hexamethonium (hex) alone or in combination with atropine methylbromide (amb) to block the heart rate (hr) response to baroreceptor activation by phenylephrine (pe) or angiotensin ii (aii)-evoked increments in blood pressure was evaluated in rats. a highly significant negative correlation existed under control conditions between the changes in map and hr. hex (10-20 mg/kg) did not influence the baroreceptor hr response of anesthetized and conscious rats, suggesting anesthesia was ...19892567154
hydrogen peroxide mutagenicity towards salmonella bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ...19892572065
nucleotide sequence of the vibrio alginolyticus glna region.the nucleotide sequence of a 4 kb fragment containing the vibrio alginolyticus glna, ntrb and ntrc genes was determined. the upstream region of the glna gene contained tandem promoters. the upstream promoter resembled the consensus sequence for escherichia coli sigma 70 promoters whereas the presumptive downstream promoter showed homology with nitrogen regulated promoters. four putative nri binding sites were located between the tandem promoters. the ntrb gene was preceded by a single presumptiv ...19892574025
[determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method].use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ...19852578835
generation of the electrochemical potential of na+ by the na+-motive nadh oxidase in inverted membrane vesicles of vibrio alginolyticus.inverted membrane vesicles prepared from vibrio alginolyticus generated a membrane potential (positive inside) and accumulated na+ by the oxidation of nadh. generation of the membrane potential required na+ and was inhibited by 2-heptyl-4-hydroxyquinoline n-oxide, a specific inhibitor of the na+-dependent nadh oxidase. collapse of the membrane potential by valinomycin stimulated the uptake of na+. in contrast, accumulation of h+ was not detected under all the conditions tested. these results sug ...19852579856
cardiopulmonary baroreflex control of brachial artery diameter in sustained essential exaggerated reflex response of forearm resistance vessels following stimulation of cardiac mechanoreceptors has been observed in human hypertension. in the present study we analyze the possibility that such an increased response also involves the large brachial artery. for that purpose, the influence of leg elevation in subjects in a supine position on brachial artery diameter and blood flow velocity was studied in 12 healthy, normal males and 17 males of the same age with sustained essential ...19892607141
purification and some properties of a novel l-2,4-diaminobutyric acid decarboxylase from vibrio alginolyticus.previous investigations have shown that members of the genus vibrio possess a novel enzyme activity decarboxylating l-2,4-diaminobutyric acid (daba) to 1,3-diaminopropane (dap). in this paper we describe the purification, by about 3600-fold, of the enzyme from v. alginolyticus. the purified enzyme was apparently homogeneous, and had a specific activity of 4200 nmol dap min-1 (mg protein)-1. the enzyme protein has an mr of 450,000 +/- 20,000 and is apparently comprised of four identical subunits ...19892614383
trends in public knowledge and attitudes about aids, south carolina, 1987-1988.the south carolina department of health and environmental control aids program assessed the state population's knowledge and attitudes about aids and hiv transmission in 1987 and 1988. each year approximately 1,800 adults were selected by a random 3-stage cluster design and asked seventeen questions by telephone about aids and hiv. questions addressed attitudes, general knowledge, hiv transmission by casual contact, hiv transmission by sex and iv drug contact, and hiv transmission by blood donat ...19892615326
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