Publications

characterization of role of the toxr gene in the physiology and pathogenicity of vibrio alginolyticus.	toxr, a conserved virulence-associated gene in vibrios, is identified in vibrio alginolyticus zj51-o, a pathogenic strain isolated from diseased fish. to reveal the role of toxr in the pathogenicity of v. alginolyticus, a deletion mutant was constructed by allelic exchange. the mutant showed the same level of growth in trypticase soy broth (tsb) and iron-limiting condition, as the wild type strain. however, deletion of toxr severely reduced resistance against bile salts and the capability of bio ...	2011	21915613
a tandem-repeat galectin involved in innate immune response of the pearl oyster pinctada fucata.	the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated pfgal) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques. the full-length cdna of pfgal was 1386bp, consisting of a 5' untranslated region (utr) of 26bp, a 3' utr of 313bp, and an open reading frame (orf) of 1047bp encoding a polypeptide of 348 amino acids with a predicted molecular weight of 38.09kda and theoretical isoelectric point of 8.49. similar to other ta ...	2011	21867976
a multidomain galectin involved in innate immune response of pearl oyster pinctada fucata.	galectins could specifically bind to β-galactoside residues and play crucial roles in innate immune responses of vertebrates and invertebrates. in this study, the cdna of a galectin with multiple carbohydrate-recognition domains (crds) was cloned from pearl oyster pinctada fucata (designated as pogal). pogal cdna was 2138bp long and consisted of a 5'-untranslated region (utr) of 120bp, a 3'-utr of 350bp with two cytokine rna instability motifs (attta), and an open reading frame (orf) of 1668bp e ...	2011	20813129
zernike phase contrast cryo-electron tomography of sodium-driven flagellar hook-basal bodies from vibrio alginolyticus.	vibrio alginolyticus use flagella to swim. a flagellum consists of a filament, hook and basal body. the basal body is made up of a rod and several ring structures. this study investigates the structure of the t ring which is a unique component of the v. alginolyticus sodium ion-driven flagellar basal body. using zernike phase contrast (zpc) cryo-electron tomography, we compared the 3d structures of purified hook-basal bodies (hbb) from a wild-type strain (kk148) and a deletion mutant lacking mot ...	2011	20705140
microarray analysis of gene expression in disk abalone haliotis discus discus after bacterial challenge.	in this study, we investigated the gene expression profiling of disk abalone, haliotis discus discus challenged by a mixture of three pathogenic bacteria vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes using a cdna microarray. upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels ≥2 or ≤2 -fold in gills and digestive tract, respectively. there were 46 tissue-specific transcripts that up-regulated specifically in the digestive ...	2011	21215803
vibrio illness in florida, 1998-2007.	this study characterized the current epidemiology of vibrio infections in florida and examined cases reported from 1998 to 2007. logistic regression was used to determine risk of death. there were 834 vibrio infections in 825 individuals (average annual incidence rate 4·8/1,000,000). common vibrio species reported were vibrio vulnificus (33%), v. parahaemolyticus (29%), and v. alginolyticus (16%). most exposures were attributed to wounds (42%), and the most common clinical syndromes were wound i ...	2011	20546636
protection of red snapper (lutjanus sanguineus) against vibrio alginolyticus with a dna vaccine containing flagellin flaa gene.	the main aims of this study were to construct a dna vaccine containing flagellin flaa gene from vibrio alginolyticus strain hy9901 and to explore the potential application of pcdna-flaa as a dna vaccine candidate for red snapper (lutjanus sanguineus).	2011	21198695
an mpeg (macrophage expressed gene) from the pacific oyster crassostrea gigas: molecular characterization and gene expression.	mpegs (macrophage expressed genes) encode members of the macpf (membrane-attack complex/perforin) protein superfamily that play essential roles in innate immunity. in the present study, a homolog of mpeg1 was identified in crassostrea gigas and designed cg-mpeg1. the complete cdna of cg-mpeg1 is 2781 bp in length, containing an orf of 2226 bp, which encodes a putative protein of 742 amino acids with a predicted 19-aa hydrophobic signal peptide, an macpf domain, and a transmembrane domain. phylog ...	2011	21272653
reverse and flick: hybrid locomotion in bacteria.		2011	21289282
passive immune-protection of small abalone against vibrio alginolyticus infection by anti-vibrio igy-encapsulated feed.	small abalone (haliotis diversicolor supertexta) is a high value-added shellfish. it however has been suffering vibrio alginolyticus infections, which cause mass death of small abalone and thus great economic losses, particularly in artificial aquaculture. in this study, we attempted to treat small abalone with anti-vibrio igy to elicit a passive immunity directly against v. alginolyticus infections. anti-vibrio igy was alginate encapsulated in egg powders as feed, which may avoid antibody inact ...	2011	21300158
enumeration of vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization.	vibrio parahaemolyticus is a gram-negative, halophilic bacterium indigenous to marine and estuarine environments and it is capable of causing food and water-borne illness in humans. it can also cause disease in marine animals, including cultured species. currently, culture-based techniques are used for quantification of v. parahaemolyticus in environmental samples; however, these can be misleading as they fail to detect v. parahaemolyticus in a viable but nonculturable (vbnc) state which leads t ...	2011	21329738
susceptibility to antibiotics of vibrio spp. and photobacterium damsela ssp. piscicida strains isolated from italian aquaculture farms.	the antibiotic resistance patterns of aetiological agents responsible for vibriosis and pasteurellosis were studied to contribute to control the spread of these two bacterial diseases in mediterranean fish farming. strains of photobacterium damsela ssp. piscicida, vibrio fluvialis, vibrio alginolyticus, vibrio parahaemolyticus, vibrio metschnikovii, isolated from italian aquaculture (fish, shellfish and crustaceans) sites, were assayed for their susceptibility to some antibacterial agents curren ...	2011	21344147
screening and characterization of a cellulase gene from the gut microflora of abalone using metagenomic library.	a metagenomic fosmid library was constructed using genomic dna isolated from abalone intestine. screening of a library of 3,840 clones revealed a 36 kb insert of a cellulase positive clone (pamhelo). a shotgun clone library was constructed using the positive clone (pamhelo) and further screening of 3,840 shotgun clones with an approximately 5 kb insert size using a congo red overlay revealed only one cellulase positive clone (pamhl9). the pamhl9 consisted of a 5,293-bp dna sequence and three ope ...	2011	21369991
antimicrobial resistance of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from farmed fish in korea from 2005 through 2007.	the antimicrobial resistance patterns to 15 antimicrobial agents of vibrio parahaemolyticus and vibrio alginolyticus isolated from farmed fishes, including olive flounder (paralichthys olivaceus), black rockfish (sebastes schlegeli), red sea bream (pagrus major), and sea bass (lateolabrax japonicus), were investigated from 2005 through 2007. a total of 218 v. parahaemolyticus isolates and 153 v. alginolyticus isolates were obtained from the 180 fish samples collected from fish farms located alon ...	2011	21375873
ktrb, a member of the superfamily of k+ transporters.	ktrb is the k(+)-translocating subunit of the k(+)-uptake system ktrab from bacteria. it is a member of the superfamily of k(+)transporters (skt proteins) with other sub-families occurring in archaea, bacteria, fungi, plants and trypanosomes. skt proteins may have originated from small k(+) channels by at least two gene duplication and two gene fusion events. they contain four covalently linked m(1)pm(2) domains, in which m(1) and m(2) stand for transmembrane stretches, and p for a p-loop, which ...	2011	21680052
molecular cloning, characterization and expression analysis of two novel tolls (lvtoll2 and lvtoll3) and three putative sp+ñtzle-like toll ligands (lvspz1-3) from litopenaeus vannamei.	toll-like receptor-mediated nf-+¦b pathways are essential for inducing immune related-gene expression in the defense against bacterial, fungal and viral infections in insects and mammals. although a toll receptor (lvtoll1) was cloned in litopenaeus vannamei, relatively little is known about other types of toll-like receptors and their endogenous cytokine-like ligand, sp+ñtzle. here, we report two novel toll-like receptors (lvtoll2 and lvtoll3) and three sp+ñtzle-like proteins (lvspz1-3) from l. ...	2011	21827783
enhanced immune defences in pacific white shrimp (litopenaeus vannamei) post-exposure to a vibrio vaccine.	this study was conducted to determine if exposure of shrimp, litopenaeus vannamei, to a commercial anti-vibrio vaccine caused changes in antibacterial and cellular (phagocytosis) defences. shrimp post-larvae were administered either vibromax™ vaccine or a blank preparation. whole body homogenates were prepared before (day 0), during (day 10) and after (day 20) vaccination and incubated with a selection of pathogenic vibrios. homogenate from day 0 animals showed natural antibacterial activity tow ...	2011	21377471
genotype analysis of collagenase gene by pcr-sscp in vibrio alginolyticus and its association with virulence to marine fish.	in this article, the genotypes for vibrio alginolyticus were analyzed based on collagenase gene by pcr-single strand conformation polymorphism (sscp) method and their relationship to the virulence for marine fish were studied by a bacteria challenging experiment. results showed total 13 genotypes designated as a1, a2, a3, a4, b, c, d, e, f, g1, g2, h, and i were identified. among them, isolates from type a1 were dominant in abundance (48.1%) and the isolates from nanyou had the highest polymorph ...	2011	21394507
biological characterization of two marine bdellovibrio-and-like organisms isolated from daya bay of shenzhen, china and their application in the elimination of vibrio parahaemolyticus in oyster.	bdellovibrio-and-like organisms (balos) are a group of highly motile delta-proteobacteria that prey on other gram-negative bacteria. however, nothing is known of the application potential of marine balos in safeguarding seafood safety. here, biological characterization of two marine balos strains and their application in the elimination of vibrio parahaemolyticus in oyster (crassostrea ariakensis) at the laboratory scale were investigated. balos strains bdh12 and bdhsh06 were isolated from sedim ...	2011	21899909
a putative g protein-coupled receptor involved in innate immune defense of procambarus clarkii against bacterial infection.	the immune functions of g protein-coupled receptor (gpcr) were widely investigated in mammals. however, limited researches on immune function of gpcrs were reported in invertebrates. in the present study, the immune functions of hp1r gene, a putative gpcr identified from red swamp crayfish procambarus clarkii were reported. expression of hp1r gene was significant up-regulated in response to heat-killed aeromonas hydrophila challenge. hp1r gene silencing mediated by rna interference significantly ...	2011	21964155
Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.	Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ...	2011	22177227
antimicrobial effects of essential oils of cinnamosma fragrans on the bacterial communities in the rearing water of penaeus monodon larvae.	farmed shrimps are vectors of various vibrio species that are considered a potential health hazard. previous study has shown that vibrio parahaemolyticus, vibrio vulnificus, and vibrio alginolyticus dominated in the water and larval samples of shrimp hatchery (randrianarivelo et al. 2010 ). the effects of two essential oils (eos) of cinnamosma fragrans, an endemic plant to madagascar (b8: linalool-type and b143: 1,8-cineole-type), were determined on the total heterotrophic aerobic bacteria and t ...	2011	21395412
sybr green i-based real-time pcr targeting the rpox gene for sensitive and rapid detection of vibrio alginolyticus.	rpox, a vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by sybr green i-based real-time pcr. the specificity of the detection was confirmed in different samples. the minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.	2011	21402144
Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract.	Peperomia pellucida leaf extract was characterized for its anticancer, antimicrobial, antioxidant activities, and chemical compositions. Anticancer activity of P. pellucida leaf extract was determined through Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7) cell line and the antimicrobial property of the plant extract was revealed by using two-fold broth micro-dilution method against 10 bacterial isolates. Antioxidant activity of the plant extract was then charact ...	2011	22071643
a smaller particle size improved the oral bioavailability of monkey head mushroom, hericium erinaceum, powder resulting in enhancement of the immune response and disease resistance of white shrimp, litopenaeus vannamei.	the effects of different particle sizes (100-150, 74-100, and <74 µm) of powder of the dried and ground stipe from the monkey head mushroom, hericium erinaceum, on the immune response and disease resistance of white shrimp, litopenaeus vannamei, against the pathogen, vibrio alginolyticus, were examined. mushroom powder with a particle size of <74 µm had a significantly higher effect on the disease resistance of shrimp compared to particle sizes of >74 µm. mortality of shrimp after being injected ...	2011	21421058
cloning and expression of toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper epinephelus coioides.	the two toll-like receptors, tlr1 and tlr2 were cloned from orange-spotted grouper, epinephelus coioides, an important teleost fish in mariculture of asia. the cdna sequences of tlr1 and tlr2 are 3195 and 3439 bp long, with an open reading frame (orf) of 2406 and 2466 bp, encoding 801 and 821 amino acids, respectively. the tlr family motifs, i.e. leucine-rich repeat (lrr) domains and toll/interleukin (il)-1 receptor (tir) domains are conserved in the tlr1 and tlr2, with nine and ten leucine-rich ...	2011	21440311
bacterial chemotaxis in an optical trap.	an optical trapping technique is implemented to investigate the chemotactic behavior of a marine bacterial strain vibrio alginolyticus. the technique takes the advantage that the bacterium has only a single polar flagellum, which can rotate either in the counter-clock-wise or clock-wise direction. the two rotation states of the motor can be readily and instantaneously resolved in the optical trap, allowing the flagellar motor switching rate s(t) to be measured under different chemical stimulatio ...	2011	21494608
[preparation and identification of ompw monoclonal antibodies].	to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw.	2011	21906472
a macrophage migration inhibitory factor like oxidoreductase from pearl oyster pinctada fucata involved in innate immune responses.	macrophage migration inhibitory factor (mif) is an important cytokine and plays a crucial role as a pivotal regulator of innate immunity. in this study, a mif cdna was identified and characterized from the pearl oyster pinctada fucata (designated as pomif). the full-length of pomif was 1544 bp and consisted of a 5'-untranslated region (utr) of 45 bp, a 3'-utr of 1139 bp with a polyadenylation signal (aataaa) at 12 nucleotides upstream of the poly (a) tail. the open reading frame (orf) of pomif w ...	2011	21496487
roles of hfq in the stress adaptation and virulence in fish pathogen vibrio alginolyticus and its potential application as a target for live attenuated vaccine.	vibrio alginolyticus has brought about severe economic damage to the mariculture industry by causing vibriosis in various fish species in south china. the virulent determinants of this bacterium have not been well characterized except the exotoxin alkaline serine protease, asp. in addition, the mechanism of virulence regulation in v. alginolyticus remains largely unknown apart from a vibrio harveyi-like quorum sensing (qs) system which is established to manipulate the expression of various virul ...	2011	21523476
cloning of a novel collagenase gene from the gram-negative bacterium grimontia (vibrio) hollisae 1706b and its efficient expression in brevibacillus choshinensis.	the collagenase gene was cloned from grimontia (vibrio) hollisae 1706b, and its complete nucleotide sequence was determined. nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kda protein of 767 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the hexxh motif. g. hollisae collagenase showed 60 and 59% amino acid sequence identities to vibrio parahaemolyticus and v ...	2011	21515782
establishment of a caenorhabditis elegans infection model for vibrio alginolyticus.	vibrio alginolyticus, a common bacterium in the marine environment, is a threat to marine animals and humans by causing serious infections. the present study reveals the establishment of a caenorhabditis elegans infection model for vibrio alginolyticus. the infection and colonization was localized in the animal by tagging v. alginolyticus with gfp and using confocal laser scanning microscopy. chemotactic response of c. elegans to v. alginolyticus, pharyngeal distention and blockage of vulval reg ...	2011	21298688
litopenaeus vannamei tumor necrosis factor receptor-associated factor 6 (traf6) responds to vibrio alginolyticus and white spot syndrome virus (wssv) infection and activates antimicrobial peptide genes.	tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a key signaling adaptor protein not only for the tnfr superfamily but also for the interleukin-1 receptor/toll-like receptor (il-1/tlr) superfamily. to investigate traf6 function in invertebrate innate immune responses, litopenaeus vannamei traf6 (lvtraf6) was identified and characterized. the full-length cdna of lvtraf6 is 2823bp long, with an open reading frame (orf) encoding a putative protein of 594 amino acids, including a ...	2011	20816892
a selective and differential medium for vibrio alginolyticus.	in this paper, we present a selective and differential medium, termed vibrio alginolyticus (val) agar, developed for the isolation and identification of v. alginolyticus. the presence of bile salts, high salinity and high incubation temperature allows the selective growth of moderately halophilic vibrio species. differentiation of bacteria is achieved by identifying species capable of sucrose fermentation, made visible by the ph indicator bromocresol purple. in this study, all of the 26 strains ...	2011	21306589
molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus trituberculatus.	an extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ...	2011	20848212
[evaluation of the ventricular function using radioisotope ventriculography in patients with impaired glucose metabolism].		2011	3228812
valproate-associated hepatotoxicity and its biochemical mechanisms.	intake of the anticonvulsant drug valproic acid, or its sodium salt, has been associated with occasional instances of severe and sometimes fatal hepatotoxicity. probably at least 80 cases have occurred worldwide. the syndrome affects perhaps 1 in 10,000 persons taking the drug, and usually develops in the early weeks or months of therapy. most instances have involved children, usually those receiving more than 1 anticonvulsant. multiple cases have occurred in 2 families. the typical presentation ...	2011	3131628
[x-ray computed tomography in venous pathology].	after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed.	2011	3043474
vibrio alginolyticus-associated wound infection acquired in british waters, guernsey, july 2011.	in july 2011, a vibrio alginolyticus infection was diagnosed in a woman from guernsey in the channel islands, british isles after sea bathing and application of a natural seaweed dressing to a pre-existing leg wound. microbiological investigation confirmed vibrio in the wound and the species of seaweed used for the dressing. the washing of open wounds in seawater and use of unsterilised seaweed dressings should be discouraged, particularly in individuals with underlying risk conditions.	2011	22027377
phlegmonous necrotising cellulitis after reduction mammoplasty. a report of 4 cases.		2011	3187790
cdna cloning and mrna expression of a tandem-repeat galectin (pogal2) from the pearl oyster, pinctada fucata.	galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. we cloned the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated as pogal2). pogal2 cdna is 1347 bp long and consists of a 5'-untranslated region (utr) of 3 bp, a 3'-utr of 297 bp with one cytokine rna instability motif (attta), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, wi ...	2011	21948759
quorum sensing and alternative sigma factor rpon regulate type vi secretion system i (t6ssva1) in fish pathogen vibrio alginolyticus.	type vi secretion system (t6ss) is a highly conserved bacterial protein secretion system and is precisely regulated in gram-negative pathogens. in vibrio alginolyticus, an important fish pathogen, two complete t6ss gene clusters (t6ssva1 and t6ssva2) were identified. in this study, expression of a hemolysin coregulated protein (hcp1), which is one of the hallmarks of t6ss, was found to be strictly regulated in this bacterium. we showed that the expression of hcp1 was growth phase-dependent and t ...	2011	22173829
Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus.	Arginine kinase (AK) is an important phosphotransferase that plays a critical role in energy metabolism in invertebrates. In this paper, the cDNA of AK (designated as PtAK) was identified from the eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA was 1,479 bp, containing an open reading frame of 1,074 bp that coded for 357 amino acids. The estimated molecular mass of mature PtAK was 40.30 kDa and theoretical isoelectric point was 6.18. Amino acid sequence alig ...	2011	22101972
Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs.	Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid prote ...	2011	22052546
a novel sialic acid binding lectin with anti-bacterial activity from the hong kong oyster (crassostrea hongkongensis).	lectins play an important role in immune recognition and host defense. in the present study, a full-length cdna encoding a novel sialic acid binding lectin was cloned from crassostrea hongkongensis (designated ch-salectin) by rapid amplification of cdna ends (race). it is 531 bp in length, containing a 21 bp 5' utr, a 39 bp 3' utr and a 468 bp orf coding for 156 amino acids. the ch-salectin protein contains a signal peptide and a conserved complement component c1q domain. the purified recombinan ...	2011	21906682
use of the romanian product silimarina in the treatment of chronic liver diseases.	the romanian product silimarina (synonym legalon) was administered in a randomized double-blind trial, to a group of 180 patients with chronic persistent hepatitis (cph), chronic active hepatitis (cah) and hepatic cirrhosis (hc). the trial lasted for 40 days. the results showed favourable effects similar with those obtained with other preparations produced by foreign drug industries. the romanian product proved to have no toxic effect. the authors discuss the present possibilities of estimating ...	2011	3072661
helping the deaf in kenya.		2011	3187393
cleavage lines of the oral mucosa in japanese cadavers.	cleavage lines were experimentally reproduced in the oral mucosa of japanese cadavers. they were investigated macroscopically and histologically. the reproduced cleavage lines showed little directional variation by sex or age. from the histological findings, the mucosal cleavage lines were interpreted to represent cleavage split along the direction of connective tissue fibres in the lamina propria.	2011	3165386
extracorporeal gas exchange in acute respiratory failure.		2011	3122975
immunoelectrophoretic demonstration of rho(d) antigen of red blood cell membrane.		2011	3095572
metastatic adenocarcinoma of the carpus: a case report.	a patient is described who presented with a painful swelling of the dorsum of the hand which arose from the hamate and proved histologically to be a secondary from a previously resected gastric adenocarcinoma.	2011	3171299
non-hodgkin's lymphoma in the elderly. a retrospective clinicopathologic study of 50 patients.	the pathologic and clinical findings of 50 patients aged 65 or older (median, 71.5 years) with non-hodgkin's lymphomas (nhl) are reported. these patients formed 27% of all cases of nhl seen in a single institution over a 7-year period. forty patients presented with nodal and 10 with extranodal nhl. according to the ann arbor system, 20 were clinical and/or pathologic stages i and ii, and 30 were stages iii and iv; of the 10 patients presenting with extranodal nhl, 8 were stages i and ii. histolo ...	2011	3697915
modification of arachidonic metabolism by flavonoids.	the pharmacological effects of many plants depend on their flavonoid constituents. this review is concerned with the influence of flavonoids on arachidonic metabolism as the possible mechanism of action of such natural products. special attention has been paid to structure-activity relationships since this knowledge may allow the synthesis of more potent and selective analogs with therapeutic applications.	2011	3126368
prevalence of mobile genetic elements and transposase genes in vibrio alginolyticus from the southern coastal region of china and their role in horizontal gene transfer.	vibrio alginolyticus has high genetic diversity, but little is known about the means by which it has been acquired. in this study, the distributions of mobile genetic elements (mges), including integrating conjugative elements (ices), superintegron-like cassettes (sics), insertion sequences (iss), and two types of transposase genes (valt1 and valt2), in 192 strains of v. alginolyticus were investigated. ice, sic, and is elements, valt1, and valt2 were detected in 8.9%, 13.0%, 4.7%, 9.4%, and 2.6 ...	2012	23844479
ulcerated yellow spot syndrome: implications of aquaculture-related pathogens associated with soft coral sarcophyton ehrenbergi tissue lesions.	we introduce a new marine syndrome called ulcerated yellow spot, affecting the soft coral sarcophyton ehrenbergi. to identify bacteria associated with tissue lesions, tissue and mucus samples were taken during a 2009 indo-pacific research expedition near the wakatobi island chain, indonesia. polymerase chain reaction targeting the 16s rdna gene indicated associations with the known fish-disease-causing bacterium photobacterium damselae, as well as multiple vibrio species. results indicate a shif ...	2012	23269388
[geriatric pharmacology].		2012	3475806
obese binge eaters: affect, cognitions, and response to behavioural weight control.		2012	3397436
a new use of instrumentation in fluoroscopy controlled heel spur surgery.		2012	3397874
patterns of plasma imipramine-desipramine concentrations in patients receiving concomitant fluphenazine decanoate.	plasma levels of imipramine and desipramine were monitored in a series of 13 patients with postpsychotic depressions who had a fixed dose of imipramine (150 mg/day) added to their clinically adjusted, stable dose of fluphenazine decanoate. despite the potential for metabolic inhibition of the tricyclic drug by the neuroleptic drug, no relationship was found between the fluphenazine dose and the subsequent plasma concentrations of imipramine and desipramine. however, plasma antidepressant levels ...	2012	3338977
sensitive non-radioactive dot-blot hybridization using dna probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.	a new labelling method for cloned dna probes used in hybridization assays is described. the dna insert of recombinant plasmid dna was made partially single-stranded for the labelling reaction by a restriction enzyme digest, followed by a controlled exonuclease iii incubation. a thiol-containing psoralen derivative was covalently bound through irradiation with uv-light to the remaining double-stranded region of the plasmid dna. the psoralen-sh groups were labelled with a large number of metal che ...	2012	3344204
[protein studies in cystic and peritoneal fluids of ovarian tumors with special reference to cea].	the levels of total protein, ig, afp, beta-hcg, cea, sc, sp1 and alpha 2-pag were measured in cystic and peritoneal effusions obtained from 70 females with benign and malignant ovarian tumors. this study was carried out to test out parameters those are useful for tumor monitoring of ovarian cancer. our results indicate that determining cea in peritoneal effusions could be one of useful methods in tumor monitoring of mucinous cystadenocarcinomas in cases of known histology and praetherapeutic est ...	2012	3376613
[dosimeter for the detection of sulfur dioxide concentrations at the work site].		2012	3798980
demonstration of the "no-reflow" phenomenon by digital coronary arteriography.		2012	3510523
routine sigmoidoscopy costs and usefulness.		2012	5108295
In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture.	Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ...	2012	22078751
oxidation of antipsoriatic 10-acyldithranol derivatives and dithranol to 1,8-dihydroxyanthraquinone.		2012	3369924
antidesmosomal monoclonal antibody in the diagnosis of intracranial tumours.	immunocytochemistry has been applied extensively to the diagnosis of intracranial tumours, but meningiomas still present a diagnostic problem. however, desmosomes have been shown by electron microscopy to be present in meningiomas, and this distinguishes them from gliomas. this paper describes a new monoclonal antibody, 11-5f, against desmosomal proteins 1 and 2 (desmoplakins) and assesses its usefulness in the diagnosis of meningiomas and other intracranial tumours. a total of 74 surgically rem ...	2012	3323433
cloning and expression analysis of recombination activating genes (rag1/2) in red snapper (lutjanus sanguineus).	recombination activating genes (rag1 and rag2), involved in the v(d)j recombination of immunoglobulin and t-cell receptor genes play a crucial role in the adaptive immune response in vertebrates. the expression of these genes was required for the proper development and maturity of lymphocytes so that they can be used as useful markers to evaluate the development of lymphoid organ. in this paper, the cdna of rag1 and rag2 in red snapper, lutjanus sanguineus were cloned by homological cloning and ...	2012	22266137
identification and characterization of a serine protease inhibitor (ptserpin) in the swimming crab portunus trituberculatus.	serine protease inhibitors (serpins) play a key role in diverse immune biological processes. a serine protease inhibitor (serpin), namely ptserpin, was identified from the haemocyte cdna library of swimming crab portunus trituberculatus. the full-length ptserpin cdna was 1593 bp, including an open reading frame (orf) of 1227 bp encoding a polypeptide of 408 amino acids with estimated molecular mass of 45.048 kda and theoretical isoelectric point of 7.23. predicted tertiary structure of ptserpin ...	2012	22245590
Dietary administration of zingerone to enhance growth, non-specific immune response, and resistance to Vibrio alginolyticus in Pacific white shrimp (Litopenaeus vannamei) juveniles.	Zingerone, one of the active components of ginger, is a phenolic alkanone with antioxidant and anti-inflammatory properties. The effects of zingerone supplementation on the growth, immunity, and disease resistance of Pacific white shrimp (Litopenaeus vannamei) juveniles were studied. Four experimental diets, including a control diet (without zingerone enrichment) and 1, 2.5, and 5 mg zingerone (kg diet)(-1) were used. After 56 days of culture, shrimp fed diets supplemented with 1, 2.5, and 5 mg ...	2012	22173270
nifedipine and thallium-201 myocardial perfusion in progressive systemic sclerosis.	heart disease in patients with progressive systemic sclerosis may be due in part to myocardial ischemia caused by a disturbance of the coronary microcirculation. to determine whether abnormalities of myocardial perfusion in this disorder are potentially reversible, we evaluated the effect of the coronary vasodilator nifedipine on myocardial perfusion assessed by thallium-201 scanning in 20 patients. thallium-201 single-photon-emission computerized tomography was performed under control condition ...	2012	3486363
gardner's syndrome with bilateral osteomas of coronoid process resulting in limited opening.		2012	3472187
thyroglobulin and 131i uptake of remaining tissue in patients with differentiated carcinoma after thyroidectomy.	in 158 thyroidectomized patients with well-differentiated non-metastatic thyroid cancer the results of serum thyroglobulin (tg) determination and 131i uptake values established immediately before radioiodine treatment (19 days after surgery) were compared. in 113 patients (72%) tg was above 6 ng ml-1 (lower limit of detection) and 131i uptake exceeded 2%. in 11 patients (7%) tg was undetectable and 131i uptake less than 2%. in these cases a radioiodine treatment was not performed; the thyroid ab ...	2012	3399222
Phenoloxidase in the scallop Chlamys farreri: Purification and antibacterial activity of its reaction products generated in vitro.	Phenoloxidase (PO) was purified from hemocytes of the scallop Chlamys farreri using native-PAGE and gel permeation column chromatography, and then substrate specificity and antibacterial activity generated from reaction products of purified PO were analyzed. The results showed purified PO had a molecular mass of 576 kDa in native-PAGE and 53 kDa in denatured PAGE, and could catalyze the substrates L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, catechol and hydroquinone suggesting it is a type ...	2012	22119576
The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi.	Among all the serine proteinase inhibitor families (SPIs), the pacifastin-related inhibitor is seldom isolated. A pacifastin-related SPI named as PtPLC was identified from the swimming crab Portunus trituberculatus in this study. The full-length of PtPLC was cloned from haemocytes cDNA library by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The PtPLC contained an open reading frame (ORF) of 1098 bp encoding a putative pacifastin-related precursor of 365 amino ...	2012	22154999
Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture.	Aims: Subcellullar localizations and cross-immunities of GAPDHs from six common pathogenic bacteria in aquaculture were investigated. Methods and Results: Subcellullar localizations of GAPDHs of Edwardsiella tarda EIB202, Edwardsiella ictaluri ATCC33202, Aeromonas hydrophila LSA34, Vibrio anguillarum MVM425, Vibrio alginolyticus EPGS020401 and Vibrio harveyi VIB647 were analysed with Western blotting, indirect immunofluorescence and flow cytometry examinations. Immunoprotections of different r ...	2012	22017642
urinary diversion: a new angle?		2012	3671701
electrohydraulic lithotripsy of gallstones in humans.		2012	3366335
13c nmr studies of carboxylate inhibitor binding to cobalt(ii) carboxypeptidase a.	both 13c nmr and electronic absorption spectral studies on cobalt(ii) carboxypeptidase a in the presence of acetate and phenylacetate provide evidence for two binding sites for each of these agents. the transverse relaxation rate t2-1 for the 13c-enriched carboxyl groups of the inhibitors is significantly increased when bound to the paramagnetic cobalt carboxypeptidase as compared to the diamagnetic zinc enzyme. the acetate concentration dependence of t2p-1 shows two inflections indicative of se ...	2012	3346659
characterization of a new phage, termed ϕa318, which is specific for vibrio alginolyticus.	vibrio alginolyticus is an opportunistic pathogen of animals and humans; its related strains can also produce tetrodotoxin and hemolysins. a new phage, ϕa318, which lysed its host v. alginolyticus with high efficiency, was characterized. the burst size of ϕa318 in v. alginolyticus was 72 pfu/bacterium at an moi of 1 at room temperature; the plaque size was as large as 5 mm in diameter. electron microscopy (em) of the phage particles revealed a 50- to 55-nm isomorphous icosahedral head with a 12- ...	2012	22327389
modulation of innate immunity and gene expressions in white shrimp litopenaeus vannamei following long-term starvation and re-feeding.	the survival rate, weight loss, immune parameters, resistance against vibrio alginolyticus and white-spot syndrome virus (wssv), and expressions of lipopolysaccharide- and ß-glucan-binding protein (lgbp), peroxinectin (px), prophenoloxidase-activating enzyme (ppa), prophenoloxidase (propo) i, propo ii, α2-macroglobulin (α2-m), integrin ß, heat shock protein 70 (hsp70), cytosolic manganese superoxide dismutase (cytmnsod), mitochondrial manganese superoxide dismutase (mtmnsod), and extracellular c ...	2012	24371579
molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (traf3) from pearl oyster pinctada fucata.	traf3 is a highly versatile regulator that negatively regulates jnk and alternative nuclear factor-κb signalling, but positively controls type i interferon production. to investigate traf3 function in innate immune responses among invertebrate especially mollusk, we characterized traf3 (pftraf3) from pearl oyster pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. pftraf3 cdna is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 5 ...	2012	22796485
zinc effect on the sea urchin paracentrotus lividus immunological competence.	pollution by heavy metals has become one of the most important problems in marine coastal areas as a consequence of anthropogenic inputs. among metal contaminants, zinc, being considered not very toxic, is sometimes released into the sea in appreciable quantities and its concentration is loosely regulated. in this work we analyzed the effects of a high zinc concentration on the sea urchin paracentrotus lividus immune system. in particular, after 24 h of zinc treatment, we evaluated coelomocytes ...	2012	22717161
orange-spotted grouper (epinephelus coioides) toll-like receptor 22: molecular characterization, expression pattern and pertinent signaling pathways.	the toll-like receptors (tlrs) are an important gene family in host innate immunologic surveillance. the tlr22 gene is an essential member of the tlrs that is only found in aquatic animals and has been detected in some bony fish. here, a tlr22 homolog, ectlr22, was characterized in the orange-spotted grouper (epinephelus coioides) via homology cloning. the 3321 bp full-length cdna sequence of ectlr22 was obtained, which included an open reading frame of 2880 bp encoding a putative peptide of 960 ...	2012	22683817
virulence genes and pathogenicity islands in environmental vibrio strains nonpathogenic to humans.	most vibrio species in autochthonous marine microbial communities, such as vibrio alginolyticus, vibrio harveyi, vibrio anguillarum among others, are considered nonpathogenic for humans. however, because many bacterial virulence genes are located in mobile genetic elements, the acquisition of mobile dna could mediate the appearance of virulent or more virulent strains even in a species defined as nonpathogenic. in this study, we screened a collection of marine nonpathogenic vibrio strains isolat ...	2012	22676367
real-time pcr optimization to identify environmental vibrio spp. strains.	to identify vibrio vulnificus, vibrio cholerae and vibrio alginolyticus using standardized dna extraction method and real-time pcr assays, among a large number of bacterial strains isolated from marine environment.	2012	22639795
modulation of the innate immune system in white shrimp litopenaeus vannamei following long-term low salinity exposure.	immune parameters, haemocyte lifespan, and gene expressions of lipopolysaccharide and β-glucan-binding protein (lgbp), peroxinectin (px), integrin β, and α2-macroglobulin (α2-m) were examined in white shrimp litopenaeus vannamei juveniles (0.48 ± 0.05 g) which had been reared at different salinity levels of 2.5‰, 5‰, 15‰, 25‰, and 35‰ for 24 weeks. all shrimp survived during the first 6 weeks. the survival rate of shrimp reared at 2.5‰ and 5‰ was much lower (30%) than that of shrimp reared at 15 ...	2012	22626810
investigation of the roles of t6ss genes in motility, biofilm formation, and extracellular protease asp production in vibrio alginolyticus with modified gateway-compatible plasmids.	the aims of this study were to create and evaluate the gateway-compatible plasmids for investigating the function of genes in vibrio alginolyticus and other gram-negative bacteria.	2012	22563695
poly d,l-lactide-co-glycolic acid-liposome encapsulated odn on innate immunity in epinephelus bruneus against vibrio alginolyticus.	the efficacy of poly d,l-lactide-co-glycolic acid (plga)-liposome (l) encapsulated oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine motifs (cpg-odns) on innate and adaptive immune response and disease resistance in kelp grouper (epinephelus bruneus) against vibrio alginolyticus at weeks 1, 2, and 4 is reported. the superoxide dismutase (sod), respiratory burst, and lysozyme activities significantly increased in e. bruneus when immunized with odn, plga+odn, l+odn, and plga+l+od ...	2012	22551979
a new type of kazal proteinase inhibitor related to shrimp penaeus (litopenaeus) vannamei immunity.	a clone encoding a four-kazal domain-containing protein was isolated from the hemostats of a penaeus vannamei cdna library. the full-length cdna sequence is 975 bp in length and encodes a 24.4 kda protein (228 residues). four kazal domains, each 43-46 residues in length, were detected in the deduced primary structure. the first, third and fourth domains have the cplreelpvc, cpavydpvc and cplyvdpvc motifs, respectively, suggesting that they are able to inhibit chymotrypsin and elastase. the mrna ...	2012	22542646
identification of the immune expressed sequence tags of pearl oyster (pinctada martensii, dunker 1850) responding to vibrio alginolyticus challenge by suppression subtractive hybridization.	one hemolymph subtracted cdna library of pearl oyster (pinctada martensii, dunker 1837) was constructed using the suppression subtractive hybridization (ssh) in response to vibrio alginolyticus. a total of 1089 clones were sequenced. all the consensuses were recognized based on the blast searches in ncbi, and revealed that 376 (58%) of them had no significant matches to reported sequences in the database. 267 ests were in significant matches after homologous sequence searches. hypothesized genes ...	2012	22515956
development and efficacy of an attenuated vibrio harveyi vaccine candidate with cross protectivity against vibrio alginolyticus.	vibrio harveyi is a gram-negative bacterial pathogen that can infect a wide range of marine animals. in previous studies, we have reported a virulent v. harveyi strain, t4d. in the present study, an attenuated mutant of t4d, t4dm, was obtained by selection of rifampicin resistance. compared to the wild type, t4dm was different in whole-cell protein profile and much slower in growth rate when cultured in stress conditions caused by iron depletion. virulence analysis showed that compared to t4d, t ...	2012	22484605
differential immune response in the hard clam (mercenaria mercenaria) against bacteria and the protistan pathogen qpx (quahog parasite unknown).	the immune response of the hard clam (quahog) mercenaria mercenaria following challenge with live bacteria (vibrio alginolyticus) and the protist qpx (quahog parasite unknown) was investigated. the study also compared immune responses following qpx challenge in two different hard clam broodstocks exhibiting different degrees of susceptibility toward this parasite. different immune and stress-related cellular and humoral factors were assessed including general hemocyte parameters (total and diffe ...	2012	22484278
downregulation of na(+)-nqr complex is essential for vibrio alginolyticus in resistance to balofloxacin.	increasingly isolated frequency of antibiotic-resistant v. alginolyticus strains in clinic and aquaculture has been reported, but the mechanisms of v. alginolyticus antibiotic resistance are largely absent. in the present study, native/sds-page based proteomics, which may provide information on protein-protein interaction, was utilized to investigate differential proteins of v. alginolyticus in resistance to balofloxacin. ten proteins were altered, in which v12g01_04671, v12g01_00457, v12g01_159 ...	2012	22465713
characterization of poma mutants defective in the functional assembly of the na(+)-driven flagellar motor in vibrio alginolyticus.	the polar flagellar motor of vibrio alginolyticus rotates using na(+) influx through the stator, which is composed of 2 subunits, poma and pomb. about a dozen stators dynamically assemble around the rotor, depending on the na(+) concentration in the surrounding environment. the motor torque is generated by the interaction between the cytoplasmic domain of poma and the c-terminal region of flig, a component of the rotor. we had shown previously that mutations of flig affected the stator assembly ...	2012	22343296
a new anti-lipopolysaccharide factor isoform (ptalf4) from the swimming crab portunus trituberculatus exhibited structural and functional diversity of alfs.	anti-lipopolysaccharide factors (alfs) are the potent antimicrobial peptides that can bind and neutralize lipopolysaccharide (lps). in this study, a new isoform of the alf homologs (ptalf4) was cloned from eyestalk cdna library of swimming crab portunus trituberculatus. ptalf4 shared lower amino acid similarity with other alfs, yet it contained the conserved lps-binding domain and was clearly member of the alf family. the genomic sequence of ptalf4 consisted of three exons interrupted by two int ...	2012	22333564
differential expression analysis of nuclear oligomerization domain proteins nod1 and nod2 in orange-spotted grouper (epinephelus coioides).	nucleotide-binding oligomerization domain-containing proteins-1 and -2 (nod1 and nod2) are members of the nod-like receptors (nlrs) family. they are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. in this study, the full-length orf sequences of nod1 and nod2 were cloned, and the putative amino acid sequences were identified in orange-spotted grouper (epinephelus coioides). the complete open reading frame (orf) of grouper nod1 con ...	2012	22982325
comparative study on the antibiotic susceptibility and plasmid profiles of vibrio alginolyticus strains isolated from four tunisian marine biotopes.	the antibiotic resistance patterns and the plasmids profiles of the predominant etiological agent responsible for vibriosis in tunisia, v. alginolyticus were studied to contribute to control their spread in some mediterranean aquaculture farms and seawater. the sixty-nine v. alginolyticus strains isolated from different marine tunisian biotopes (bathing waters, aquaculture and conchylicole farms and a river connected to the seawater during the cold seasons) were multi-drug resistant with high re ...	2012	22918722
withdrawn: effects of saponin supplementation diet on hematological, biochemical, and immunological responses in epinephelus bruneus against vibrio alginolyticus.	this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.	2012	22906763
molecular cloning and characterization of class i nf-κb transcription factor from pearl oyster (pinctada fucata).	nf-κb transcription factors play central roles in many important physiological and pathological processes including innate immune responses. here we report the cloning of an nf-κb transcription factor, pfrelish from pearl oyster pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. pfrelish full-length cdna is 3916 bp with an open reading frame of 3558 bp encoding a putative protein of 1186 amino acids. the deduced pfrelish contains a n-terminal rhd, a nucleu ...	2012	22796487