mutational analysis of the gtp-binding motif of flhf which regulates the number and placement of the polar flagellum in vibrio alginolyticus.precise regulation of the number and placement of flagella is critical for the mono-flagellated bacterium vibrio alginolyticus to swim efficiently. we previously proposed a model in which the putative gtpase flhf determines the polar location and generation of the flagellum, the putative atpase flhg interacts with flhf to prevent flhf from localizing to the pole, and thus flhg negatively regulates the flagellar number in v. alginolyticus cells. to investigate the role of the gtp-binding motif of ...200919605463
identification of broad cross-protective immunogens using heterogeneous antiserum-based immunoproteomic approach.bacterium is still one of the major causes of life-threatening microbial infections. the most effective way to control bacterial infections is probably vaccine prevention. however, development of bacterial vaccine, especially polyvalent vaccines that could be used to fight against a variety of bacterial serotypes and species, is challenging due to the difficulty in identifying broad cross-protective antigens for different serotypes and species of pathogenic bacteria. in the present study, we dev ...200919640004
endophthalmitis caused by vibrio alginolyticus.vibrio alginolyticus is a facultative anaerobic gram-negative bacillus found in normal marine flora. ocular infections induced by v. alginolyticus are extremely rare. we report a case of endophthalmitis caused by v. alginolyticus to draw attention to v. alginolyticus infections following ocular injuries.200919710275
characteristics of na-dependent respiratory chain in vibrio anguillarum, a fish pathogen, in comparison with other marine vibrios.the activity of membrane-bound nadh oxidase of vibrio anguillarum m93 (serotype j-o-1), which causes vibriosis in freshwater area was activated by na(+) in the same manner as other marine vibrios. however, in addition to na(+), k(+) was also found to positively enhance the nadh oxidase activity of strain m93. this tendency has not been recognized in other marine vibrios. furthermore, the na(+)-dependent nadh oxidase of strain m93 required less na(+) (0.1 m) for its maximum activity than those of ...200319719639
alterations of outer membrane proteins and virulence genes expression in gamma-irradiated vibrio parahaemolyticus and vibrio alginolyticus.gamma-irradiation technology sterilizes microorganisms and thereby prevents decay and improves the safety and shelf stability of food products. in this study we treated the foodborne pathogens vibrio parahaemolyticus and vibrio alginolyticus with gamma-irradiation (0.5 kgy) to evaluate their adaptative response. outer membrane protein patterns of irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. these modifications were manifested ...200919735197
extraintestinal vibrio infections in mauritius.few extraintestinal vibrio infections have been reported in the african region. we report 3 cases from mauritius: one case of vibrio alginolyticus otitis externa; one case of soft tissue infection caused by non-o1 vibrio cholerae and vibrio parahaemolyticus; and one fatal case of non-o1 v. cholerae cellulitis and septicaemia.200819745511
molecular cloning of mud crab scylla serrata peroxinectin and its expression following vibrio alginolyticus and peptidoglycan injections. 201019751834
cdna cloning, identification, tissue localisation, and transcription profile of a transglutaminase from white shrimp, litopenaeus vannamei, after infection by vibrio alginolyticus.complementary (c)dna encoding transglutaminase (tg) messenger (m)rna of white shrimp, litopenaeus vannamei, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the tg sequence of the horseshoe crab, tachypleus tridentatus (accession no.: baa02134); tiger shrimp, penaeus monodon (aal78166); and pacifastacus leniusculus (af336805). the 2638-bp cdna contained an open reading frame ...200919782141
immune parameters in the humoral fluids of amphioxus branchiostoma belcheri challenged with vibrio alginolyticus.the knowledge concerning the humoral immunity is scarce in amphioxus branchiostoma belcheri. this study measured the humoral parameters including lysozyme, antimicrobial activity, microbial agglutinin and haemagglutinin in amphioxus humoral fluids before and after vibrio alginolyticus challenge. after challenged with v. alginolyticus, the lysozyme activity, growth inhibiting activities against escherichia coli and v. alginolyticus and microbial agglutinating activities against micrococcus lysode ...201019800008
molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (portunus trituberculatus).hsp70 can stimulate cells of the innate immune system directly by acting as "danger"-signaling molecules. to understand the immune defense mechanisms of swimming crab portunus trituberculatus (decapoda: brachyura: portunidae), the cdna of hsp70 (designated pthsp70) was cloned by the combination of homology cloning and rapid amplification of cdna ends (race). the full-length pthsp70 cdna was 2195 bp, including an open reading frame (orf) of 1950 bp encoding a polypeptide of 650 amino acids with e ...201019815077
conformational and dynamics simulation study of antimicrobial peptide hedistin-heterogeneity of its helix-turn-helix motif.hedistin is an antimicrobial peptide isolated from the coelomocytes of nereis diversicolor, possessing activity against a large spectrum of bacteria including the methicillin resistant staphylococcus aureus and vibrio alginolyticus. the three-dimensional structure of hedistin in both aqueous solution and deuterated dodecylphosphocholine (dpc) micelles was examined using circular dichroism (cd) and nuclear magnetic resonance (nmr) techniques. and, the early events of the antibacterial process of ...200919819221
innate immunity of the white shrimp litopenaeus vannamei weakened by the combination of a vibrio alginolyticus injection and low-salinity stress.white shrimp litopenaeus vannamei were reared at a salinity of 35 per thousand without a vibrio alginolyticus injection (unchallenged group), and other shrimp were reared at 35 per thousand, injected with tryptic-soy broth (tsb)-grown v. alginolyticus at 1.8 x 10(5) colony-forming units (cfu) shrimp(-1) (challenged group), and then examined for the hyaline cell (hc) count, granular cell (gc, including semi-granular cell) count, total haemocyte count (thc), phenoloxidase (po) activity, respirator ...201019819336
molecular cloning and characterization of prophenoloxidase gene in swimming crab portunus trituberculatus.phenoloxidase (po), a melanin-synthesizing enzyme, found as a zymogen (propo) in hemolymp, plays an important role in arthropod defence. in this study, a prophenoloxidase (propo) cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the cdna clone had a full-length of 3040 bp, with an open reading frame of 2019 ...201019835959
molecular cloning and characterization of a cytosolic manganese superoxide dismutase (cytmnsod) and mitochondrial manganese superoxide dismutase (mtmnsod) from the kuruma shrimp marsupenaeus japonicus.a cytosolic manganese superoxide dismutase (cytmnsod) gene and a mitochondrial manganese superoxide dismutase (mtmnsod) gene were cloned from the kuruma shrimp marsupenaeus japonicus using reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) method. the open reading frame (orf) of cytmnsod is 861 bp and encodes a 287 amino acids (aa) protein with a 61 aa leader sequence, whereas the orf of mtmnsod is 663 bp and encodes a 221 aa protein with a 21 aa ...201019835962
isolation of basal bodies with c-ring components from the na+-driven flagellar motor of vibrio investigate the na(+)-driven flagellar motor of vibrio alginolyticus, we attempted to isolate its c-ring structure. flig but not flim copurified with the basal bodies. flim proteins may be easily dissociated from the basal body. we could detect flig on the ms ring surface of the basal bodies.201019880601
simultaneous differential detection of human pathogenic and nonpathogenic vibrio species using a multiplex pcr based on gyrb and pnta develop a multiplex pcr targeting the gyrb and pnta genes for vibrio species differentiation.201019891709
interaction between na+ ion and carboxylates of the poma-pomb stator unit studied by atr-ftir spectroscopy.bacterial flagellar motors are molecular machines powered by the electrochemical potential gradient of specific ions across the membrane. the poma-pomb stator complex of vibrio alginolyticus couples na(+) influx to torque generation in this supramolecular motor, but little is known about how na(+) associates with the poma-pomb complex in the energy conversion process. here, by means of attenuated total reflection fourier-transform infrared (atr-ftir) spectroscopy, we directly observed binding of ...200919894756
investigation of several virulence properties among vibrio alginolyticus strains isolated from diseased cultured fish in tunisia.we analysed 34 vibrio alginolyticus strains isolated from gilthead sea bream sparus aurata l. and sea bass dicentrarchus labrax l. cultured in fish farms on the tunisian mediterranean coast for the presence of several virulence properties such as extracellular products (ecp) production, growth in iron-limiting conditions and survival in fish serum. the results obtained with different substrates showed that ecp of v. alginolyticus were hydrolytic. the virulence was correlated with the ability of ...200919899346
isolation of vibrio alginolyticus and vibrio splendidus from captive-bred seahorses with disease symptoms.vibrio species isolated from diseased seahorses were characterized by pcr amplification of repetitive bacterial dna elements (rep-pcr) and identified by 16s ribosomal rna gene sequence analysis. the results demonstrated that vibrio alginolyticus and vibrio splendidus were predominant in the lesions of these seahorses. to our knowledge, this is the first time that these bacterial species have been associated with disease symptoms in captive-bred seahorses.201019921544
defensin from disk abalone haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection.gene-encoded antimicrobial peptides (amps) serve a major role in host defense systems against pathogens. in this study, cdna of a new mollusk defensin was identified from a normalized cdna library constructed from whole tissues of disk abalone. abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. the presence of an invertebrate defensin family domain, an arrangement of six cysteine ...201019922800
expression, purification and antibody preparation of flagellin flaa from vibrio alginolyticus strain hy9901.the main aims of this study were to clone and express flagellin flaa gene from vibrio alginolyticus strain hy9901, also to prepare mouse anti-flaa polyclonal antibody for future pathogen or vaccine study.201019951364
[development of taqman real-time pcr in detection of aeromonas hydrophila].to develop a taqman real-time pcr for the detection of aeromonas hydrophila.200919954074
the implication of yggt of escherichia coli in osmotic escherichia coli mutant lacking three major k(+) uptake systems, trk, kup, and kdp, did not grow under low k(+)and high na(+) concentrations. the introduction of fkua and of fkub of a marine bacterium, vibrio alginolyticus, has been reported to compensate for the growth defect by accelerating the rate of k(+) uptake (nakamura, katoh, shimizu, matsuba, and unemoto, biochim. biophys. acta, 1277, 201-208 (1996)). we investigated the function of unknown genes of e. coli, yggs and yggt, homologs o ...200919966467
expression of hsp60 and hsp70 in white shrimp, litopenaeus vannamei in response to bacterial the present study, cdna encoding a heat shock protein 60 (lvhsp60) gene in litopenaeus vannamei was cloned using a combination of homology and rapid amplification of cdna end (race) methods. the full length of the lvhsp60 cdna was found to be 2379bp, with a 1737bp open reading frame. the translated amino acid sequence consisted of 579 residues with a calculated molecular mass of 60.8kd and an isoelectronic point (pi) of 5.97. comparison of the deduced amino acid sequence showed that it has hi ...201020043915
rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry.vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ...201020059616
disk abalone (haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: molecular cloning and immune response against bacterial infection.a novel antistasin-like cdna homologue named as ab-antistasin was isolated from the disk abalone haliotis discus discus normalized cdna library. the ab-antistasin (1398-bp) consisted of an 1185-bp open reading frame encoding 395 amino acid (aa) residues. the predicted molecular mass and isoelectric point of ab-antistasin was 44 kda and 8.5, respectively, and showed highest identity (23.1%) to hydra magnipapillata antistasin. the most striking feature of ab-antistasin is the 12-fold internal repe ...201020060477
cloning, identification, and characterization of the rpos-like sigma factor rpox from vibrio alginolyticus.vibrio alginolyticus zj-51 displays phase variation between opaque/rugose colonies (op) and translucent/smooth colonies (tr). these colony variants show great differences in biofilm formation and motility. in this study, a gene encoding for an rpos-like sigma factor, rpox, has been cloned and characterized. the absence of rpox did not affect colony switching rate but did decrease biofilm formation in both the op and the tr variants. when challenged with hydrogen peroxide, the deltarpox in the op ...200920069110
gain of function mutations in membrane region m2c2 of ktrb open a gate controlling k+ transport by the ktrab system from vibrio alginolyticus.ktrb, the k(+)-translocating subunit of the na(+)-dependent bacterial k(+) uptake system ktrab, consists of four m(1)pm(2) domains, in which m(1) and m(2) are transmembrane helices and p indicates a p-loop that folds back from the external medium into the cell membrane. the transmembrane stretch m(2c) is, with its 40 residues, unusually long. it consists of three parts, the hydrophobic helices m(2c1) and m(2c3), which are connected by a nonhelical m(2c2) region, containing conserved glycine, ala ...201020097755
functional transfer of an essential aspartate for the ion-binding site in the stator proteins of the bacterial flagellar motor.rotation of the bacterial flagellar motor exploits the electrochemical potential of the coupling ion (h(+) or na(+)) as its energy source. in the marine bacterium vibrio alginolyticus, the stator complex is composed of poma and pomb, and conducts na(+) across the cytoplasmic membrane to generate rotation. the transmembrane (tm) region of pomb, which forms the na(+)-conduction pathway together with tm3 and tm4 of poma, has a highly conserved aspartate residue (asp24) that is essential for flagell ...201020122938
prevalence of listeria, aeromonas, and vibrio species in fish used for human consumption in turkey.a total of 78 raw retail fish samples from 30 freshwater and 48 marine fish were examined for the presence of listeria, aeromonas, and vibrio species. the overall incidence of listeria spp. was 30% in freshwater samples and 10.4% in marine fish samples. listeria monocytogenes (44.5%) was the most commonly isolated species in freshwater fish, and listeria murrayi (83.5%) was the most commonly isolated species in marine fish samples. motile aeromonads were more common in marine fish samples (93.7% ...201020132688
administration of the hot-water extract of spirulina platensis enhanced the immune response of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.white shrimp litopenaeus vannamei which had been injected with the hot-water extract of spirulina platensis at 6, 10, and 20 microg g(-1), or immersed in aerated seawater containing extract at 200, 400, and 600 mg l(-1) were challenged with vibrio alginolyticus at 1.5 x 10(6) or 1.4 x 10(6) colony-forming units (cfu) shrimp(-1), and then placed in seawater. survival rates of shrimp that received the extract of s. platensis at 6-20 microg g(-1), and those of shrimp immersed in seawater containing ...201020139007
the effects of elevated carbon dioxide levels on a vibrio sp. isolated from the deep-sea.introduction: the effect of oceanic co2 sequestration was examined exposing a deep-sea bacterium identified as vibrio alginolyticus (9na) to elevated levels of carbon dioxide and monitoring its growth at 2,750 psi (1,846 m depth). findings: the wild-type strain of 9na could not grow in acidified marine broth below a ph of 5. the ph of marine broth did not drop below this level until at least 20.8 mm of co2 was injected into the medium. 9na did not grow at this co2 concentration or higher concent ...201020140649
molecular evidence for the existence of lipopolysaccharide-induced tnf-alpha factor (litaf) and rel/nf-kb pathways in disk abalone (haliotis discus discus).the lipopolysaccharide-induced tnf-alpha factor (litaf) and rel family nuclear factor kappab (rel/nf-kb) are two important transcription factors which play major roles in the regulating inflammatory cytokine, apoptosis and immune related genes. here, we report the discovery of disk abalone litaf (ablitaf) and rel/nf-kb (abrel/nf-kb) homologues and their immune responses. full-length cdna of ablitaf consists of 441 bp open reading frame (orf) that translates into putative peptide of 147 aa. analy ...201020153832
molecular cloning, characterization and mrna expression of two antibacterial peptides: crustin and anti-lipopolysaccharide factor in swimming crab portunus this study, the cdnas of a crustin and an anti-lipopolysaccharide factor (alf) were cloned from the hemocytes of swimming crab portunus trituberculatus using rapid amplification of cdna ends (race) approaches. the full-length cdna of p. trituberculatus crustin (denoted as ptcrustin) was of 584 bp, including an open reading frame (orf) of 333 bp encoding a 21 amino acids signal peptide and a mature polypeptide of 89 amino acids with the predicted molecular mass of 10.08 kda. the typical conser ...201020167286
a shared antigen among vibrio species: outer membrane protein-ompk as a versatile vibriosis vaccine candidate in orange-spotted grouper (epinephelus coioides).the outer membrane protein-ompk has been considered as a vaccine candidate for the prevention of infections due to vibrio harveyi, vibrio alginolyticus and vibrio parahaemolyticus in fish. interestingly, the polyclonal antibody raised against the recombinant ompk from v. harveyi strain ecgs020802 recognized the ompk homologues from other strains of vibrio species by immunoblotting. the ompk genes from 19 vibrio strains including v. harveyi (11), v. alginolyticus (6) and v. parahaemolyticus (2) w ...201020170736
vibrio alginolyticus mvin is a luxo-regulated protein and affects cytotoxicity toward epc cell.vibrio alginolyticus, a gram-negative marine bacterium, is one of the causative agents of fish vibriosis. its virulence factors and pathogenesis mechanism are barely known except for some extracellular products (ecps), which are implicated to be regulated by quorum sensing system. in the present study, microarray was used to analyze the transcription profiles of v. alginolyticus wild-type and a deletion mutant of luxo, the pivotal regulator in vibrio quorum sensing systems, and a putative virule ...201020208429
loop-mediated isothermal amplification method for rapid detection of vibrio alginolyticus, the causative agent of vibriosis in mariculture fish.the purpose of this study was to develop a loop-mediated isothermal amplification (lamp) method for the rapid, sensitive and simple detection of vibrio alginolyticus in mariculture fish.201020214730
differentially expressed outer membrane proteins of vibrio alginolyticus in response to six types of antibiotics.vibrio alginolyticus is an opportunistic pathogen that occasionally causes life-threatening infections in individuals and results in great losses in marine aquacultures of crustaceans and fish. recently, antibiotic-resistant strains of the bacterium from clinical and environmental sources have been reported with increasing frequency. however, few reports were involved in the antibiotic resistance of this bacterium at molecular levels. in the present study, western blotting was utilized to invest ...201020217167
isolation and experimental infection with vibrio alginolyticus in the sea horse, hippocampus reidi ginsburg, 1933 (osteichthyes: syngnathidae) in brazil.the aim of this study was to evaluate the pathogenicity of vibrio alginolyticus isolated from an outbreak of sea horse hippocampus reidi reared in the state of santa catarina, brazil, by experimental infection. sea horses with necrosis on the mouth epithelium were collected from aquaria at the aquaculture department, ufsc and the bacterium isolated from the mouth, liver, heart and blood in thiosulphate citrate bilesalt sucrose agar broth. the strains were identified by api 20e kit with 99.1% pro ...201020231979
characterization of chitinolytic bacteria and histological aspects of shell disease syndrome in european spiny lobsters (palinurus elephas) (fabricius 1787).the present research reports the first description of shell disease syndrome in european spiny lobsters palinurus elephas (fabricius 1787), which occurred in an experimental aquaculture facility in sicily (italy). both bacterial characterization and histopathological examination of the exoskeleton at site of lesions was carried out. infected specimens showed tail fan erosions, and in one case uropod ulceration and complete loss of periods. identified species included: listonella anguillarum 50.5 ...201020361978
pathogenic vibrio species in dutch shellfish destined for direct human consumption.vibrio parahaemolyticus is a common cause of shellfish-related gastroenteritis all over the world. v. parahaemolyticus and vibrio alginolyticus have previously been detected in water samples from the oosterschelde, a large inlet on the north sea, which is used for both recreational purposes and shellfish production. in 2006, oysters (crassostrea gigas) from a noncommercial oyster bed in the oosterschelde and oysters bought in dutch fish shops were tested for the presence of pathogenic vibrio spe ...201020377964
[complete nucleotide sequence of a plasmid pvae259 from vibrio alginolyticus and analysis of molecular biological characteristic of the plasmid].to obtain the complete nucleotide sequence of the plasmid pvae259 from vibrio alginolyticus, to analyze molecular characteristic of the plasmid, and to explore its potential function.201020387457
[fabrication and evaluation of the enzyme immunosensor for rapid detection of vibrio parahaemolyticus based on chitosan-sio2 hybrid membrane].to improve the key technology of immunesensors in immobilizing bio-sensitive element and keeping its bioactivity, an enzyme immunosensor based on chitosan-sio(2) (cs-sio(2)) hybrid membrane was fabricated. to estimate the new immunosensor vibrio parahaemolyticus which was the main pathogens of aquatic products.201020388366
the expression of protein disulfide isomerase from litopenaeus vannamei hemocytes is regulated by bacterial inoculation.a partial clone encoding a member of the protein disulfide isomerase (pdi) was isolated from a litopenaeus vannamei hemocyte cdna library. the 5?-end sequence was obtained by race. the complete sequence encodes for a 502-residues protein that contains two thioredoxin domains and the typical endoplasmic reticulum retention kdel motif. shrimp pdi is highly similar to the homologue protein described in both vertebrates and invertebrates. changes in the shrimp pdi mrna expression were observed after ...200920403767
molecular cloning and characterization of a novel c-type lysozyme gene in swimming crab portunus trituberculatus.lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections. in this study, the full-length cdna of a novel c-type lysozyme was cloned from the haemocytes of swimming crab portunus trituberculatus using rapid amplification of cdna ends (race) approaches. the full-length cdna of lysozyme was of 984 bp, containing a 5' untranslated region (utr) of 90 bp, a 3' utr of 222 bp with a poly (a) tail, and an open reading frame (orf) of 672 bp encoding an 18 ami ...201020420912
the protective immunity of white shrimp litopenaeus vannamei that had been immersed in the hot-water extract of gracilaria tenuistipitata and subjected to combined stresses of vibrio alginolyticus injection and temperature change.white shrimp litopenaeus vannamei which had been immersed in seawater (35 per thousand) containing the hot-water extract of gracilaria tenuistipitata at 0 (control), 400, and 600 mg l(-1) for 3 h, were subjected to temperature transfer (28 degrees c), or combined stresses of vibrio alginolyticus injection (2.4 x 10(6) colony-forming unit shrimp(-1)) and temperature transfer (28 degrees c) from 24 degrees c, and the immune parameters including hyaline cells (hcs), granular cells (gcs, including s ...201020420916
expression, characterization and immunogenicity of flagellin flac from vibrio alginolyticus strain hy9901.vibrio alginolyticus is one of ubiquitous pathogens infecting human and marine animals. flagellins of bacteria play an important role in infecting animals and inducing host immune response. in the present research, flagellin flac gene of v. alginolyticus strain hy9901 was cloned and expressed. the open reading frame of flac gene contains 1155 bp and the putative protein consists of 384 amino acid residues. polyclonal antibodies were raised in mouse against the purified recombinant flac protein a ...201020420917
characterization and expression analysis of ef hand domain-containing calcium-regulatory gene from disk abalone: calcium homeostasis and its role in immunity.the complete amino acid sequence of a calcium-regulatory gene (denoted as ab-careg i) was identified from the disk abalone haliotis discus discus cdna library. the ab-careg i is composed of 176 amino acids and the calculated molecular mass and isoelectric point were 20 and 4.2, respectively. the sequence homology of ab-careg i was 28-30 and 18-27% of known calmodulin and troponin c, respectively. four characteristic calcium-binding ef hand motifs with some modifications at conserved positions of ...201020420919
new angucyclines and antimicrobial diketopiperazines from the marine mollusk-derived actinomycete saccharothrix espanaensis an 113.two new angucyclines, saccharothrixmicines a (2) and b (3), together with three known diketopiperazines 4-6 were isolated from the actinomycete saccharothrix espanaensis an 113 associated with the marine mollusk anadara broughtoni. their structures were determined by hresi-ms and 1d and 2d nmr. compounds 2 and 3 differ in aglycone and glycosidic bond type. 2 is an alpha-l-6-deoxyaltrose-phenylglycoside of a benz[a]anthraquinone aglycone, while 3 is an o-glycoside of the same sugar linked to c-7 ...201020433079
allograft inflammatory factor-1 in disk abalone (haliotis discus discus): molecular cloning, transcriptional regulation against immune challenge and tissue, we report the identification and characterization of allograft inflammatory factor-1 (aif-1) from disk abalone haliotis discus discus that was denoted as abaif-1. the full-length cdna of abaif-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kda molecular mass. analysis of abaif-1 sequence showed that it shares characteristic two ef hand ca(+2)-binding motifs. results from phylogenetic analysis further confirm that abaif-1 is a member of the aif-1 family similar to inve ...201020435145
molecular characterization and expression analysis of interferon-gamma-inducible lysosomal thiol reductase (gilt) gene from pearl oyster pinctada fucata.interferon-gamma-inducible lysosomal thiol reductase (gilt) is an important thiol reductase, involved in class, mhc-restricted antigen processing by catalyzing disulfide bond reduction in mammals. herein, we describe the identification and characterization of pearl oyster pinctada fucata gilt (designated as pogilt). the pogilt cdna was 1273bp long and consisted of a 5'-untranslated region (utr) of 24bp, a 3'-utr of 484bp with two cytokine rna instability motifs (attta), and an open reading frame ...201020444427
identification of non-coding rnas in environmental vibrios.the discovery of non-coding rna (ncrna) has been mainly limited to laboratory model systems and human pathogenic bacteria. in this study, we begin to explore the ncrna diversity in four recently sequenced environmental vibrio species (vibrio alginolyticus 40b, vibrio communis 1da3, vibrio mimicus vm573 and vibrio campbellii baa-1116) by performing in silico searches using infernal and rfam for the identification of putative ncrna-encoding genes. this search method resulted in the identification ...201020447992
an in vitro and in vivo assessment of the potential of vibrio spp. as probiotics for the pacific white shrimp, litopenaeus vannamei.the objective of the work was to determine whether known strains of nonpathogenic vibrios can act as probiotics for the control of vibrio infections in the pacific white shrimp, litopenaeus vannamei.201020477892
cloning, expression, and genus-specificity analysis of 28-kda ompk from vibrio alginolyticus.some vibrio species are universal marine pathogens and vibrio infections are often encountered due to consumption of raw or uncooked seafood. the outer membrane proteins, playing a key role in interaction between bacteria and hosts, are potential candidates for development of vaccine and markers of the genus vibrio. in this study, the ompk (outer membrane protein k) genes of vibrio alginolyticus, v. vulnificus, v. parahaemolyticus, v. fluvialis, and v. mimicu were cloned with 798 to 822 nucleoti ...201020546410
vibrio illness in florida, 1998-2007.this study characterized the current epidemiology of vibrio infections in florida and examined cases reported from 1998 to 2007. logistic regression was used to determine risk of death. there were 834 vibrio infections in 825 individuals (average annual incidence rate 4·8/1,000,000). common vibrio species reported were vibrio vulnificus (33%), v. parahaemolyticus (29%), and v. alginolyticus (16%). most exposures were attributed to wounds (42%), and the most common clinical syndromes were wound i ...201120546636
molecular characterization and expression analysis of cathepsin l1 cysteine protease from pearl oyster pinctada fucata.cathepsin l is one of the crucial enzyme superfamilies and involved in the immune responses. in this study, a cdna encoding cathepsin l cysteine protease was identified and characterized from pearl oyster pinctada fucata (designated as pocl1). the pocl1 cdna was 1160 bp long and consisted of a 5'-untranslated region (utr) of 15 bp, a 3'-utr of 149 bp with a polyadenylation signal (aataaa) at 11 nucleotides upstream of the poly(a) tail, and an open reading frame (orf) of 996 bp encoding a polypep ...201020573562
membrane region m2c2 in subunit ktrb of the k+ uptake system ktrab from vibrio alginolyticus forms a flexible gate controlling k+ flux: an electron paramagnetic resonance study.transmembrane stretch m(2c) from the bacterial k(+)-translocating protein ktrb is unusually long. in its middle part, termed m(2c2), it contains several small and polar amino acids. this region is flanked by the two alpha-helices m(2c1) and m(2c3) and may form a flexible gate at the cytoplasmic side of the membrane controlling k(+) translocation. in this study, we provide experimental evidence for this notion by using continuous wave and pulse epr measurements of single and double spin-labeled c ...201020573964
the type iii secretion system of vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells.vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in humans as well as marine animals. however, the virulence mechanisms for this species of vibrio have not been elucidated. this study characterized multiple mechanisms that induce cell death in fish cells upon infection with a v. alginolyticus strain, zjo. the bacterium required its type iii secretion system (t3ss) to cause rapid death of infected fish cells. dying cells exhibited some feature ...201020576689
tumor suppressor qm-like gene from disk abalone (haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge.we describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor qm-like protein, abqm, in the disk abalone haliotis discus discus. the full-length cdna (765-bp) of abqm was found to consist of a 654-bp orf coding for a 218 amino acid protein of a 25 kda molecular mass with a 10.2 isoelectric point. analysis of abqm sequence revealed the presence of characteristic motifs, including the ribosomal protein l10 signature, sh3-binding motif and two antibiotic ...201020580829
deletion of valr, a homolog of vibrio harveyiś luxr generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in vibrio alginolyticus.a previous study has shown that vibrio alginolyticus zj-51 undergoes colony phase variation between opaque/rugose (op) and translucent/smooth (tr). the ai-2 quorum-sensing master regulator valr, a homolog to v. harveyi luxr, was suggested to be involved in the transition. to investigate the role of valr in the variation and in biofilm formation, an in-frame deletion of valr in both op and tr backgrounds was carried out. the mutants in both backgrounds showed an intermediate colony morphotype, wh ...201020582761
immune response in lutjanus erythropterus induced by the major outer membrane protein (ompu) of vibrio alginolyticus.the outer membrane proteins (omps) of the marine aquatic animal pathogen vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. in this study, the major 35.6 kda omp of v. alginolyticus was isolated by gel excision from the crude omp fraction from v. alginolyticus. the sequence of the first 27 amino acid residues from the n-terminal end of the protein is atv ykd ggt ell vgg rve frg dfi gsd, which has high homology with ...201020597431
molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab portunus trituberculatus.serine proteinase homologues (sphs), as one of prophenoloxiase-activating factors (ppafs), play critical roles in innate immunity of crabs. based on an est from the eyestalk full length cdna library, the complete cdna (designated as ptsph) and genomic dna of sph from the swimming crab portunus trituberculatus were cloned in this study. the estimated molecular weight of mature ptsph (354 amino acids) was 38.7 kda and its isoelectric point was 5.08. multiple sequence alignment revealed that ptsph ...201020633654
screening and selection of stress resistant lactobacillus spp. isolated from the marine oyster (crassostrea gigas).we attempted to isolate lactobacillus spp. from the marine oyster (crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. a total of 83 lactobacilli strains were isolated from oyster. they were all gram-positive, rod-shaped and catalase-negative. by performing a stress resistance assay, we selected eighteen isolates. based on 16s rrna gene sequencing, lactobacillus paracasei was the most prevalent species among the selected isolates. ...201020633689
evaluation of immune response and resistance to diseases in tiger shrimp, penaeus monodon fed with biofilm of vibrio alginolyticus.immune response in juvenile tiger shrimp, penaeus monodon fed with biofilm (bf) and free cells (fc) of vibrio alginolyticus was studied by evaluating the hemocyte count, phenoloxidase activity and antibacterial activity. the above immune responses were higher in bf fed shrimp than that in fc fed or control shrimp. among the different doses of bf of v. alginolyticus tested, 10(9) cfu g(-1) shrimp day(-1) for two weeks could evoke higher immune response. bf fed shrimp were more resistant to inject ...201020638481
identification of vaccine candidates from differentially expressed outer membrane proteins of vibrio alginolyticus in response to nacl and iron limitation.vibrio alginolyticus is the etiological agent that causes great losses in aquacultures and clinical emanating cases in humans. identification of highly efficient vaccine candidates to control v. alginolyticus infection has been highly concerned since vaccines offer a powerful approach to provide efficient protection from bacterial infections. in the present study, we firstly investigated the altered outer membrane proteins (om proteins) of v. alginolyticus in response to nacl concentrations and ...201020659563
expression, purification, and characterization of thermolabile hemolysin (tlh) from vibrio alginolyticus.hemolysin is a putative pathogenicity factor in many bacterial pathogens. in this study, a dna fragment containing the open reading frame (1254 bp) of the thermolabile hemolysin gene (tlh) from vibrio alginolyticus v05 was amplified and cloned into the expression plasmid pet-24d(+). the deduced amino acid sequence of the thermolabile hemolysin (tlh) shared 94 and 83% identity with the lecithin-dependent hemolysin (ldh)/tlh of v. parahaemolyticus and v. harveyi thermolabile hemolysin (vhh), respe ...201020662368
comparative genomic analyses identify the vibrio harveyi genome sequenced strains baa-1116 and hy01 as vibrio campbellii.three notable members of the harveyi clade, vibrio harveyi, vibrio alginolyticus and vibrio parahaemolyticus, are best known as marine pathogens of commercial and medical import. in spite of this fact, the discrimination of harveyi clade members remains difficult due to genetic and phenotypic similarities, and this has led to misidentifications and inaccurate estimations of a species' involvement in certain environments. to begin to understand the underlying genetics that complicate species leve ...201020686623
brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna from disk abalone haliotis discus discus.a brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna was cloned from the disk abalone (haliotis discus discus) and designated as ablect-1. a full-length (705 bp) of ablect-1 cdna was composed of a 576 bp open reading frame that translates into a putative peptide of 192 amino acids. deduced amino acid sequence of ablect-1 had 15.5- and 27.8% identity and similarity to human lect-1, respectively. quantitative real-time pcr analysis results showed that the mrna of ablect-1 was ...201020688173
zernike phase contrast cryo-electron tomography of sodium-driven flagellar hook-basal bodies from vibrio alginolyticus.vibrio alginolyticus use flagella to swim. a flagellum consists of a filament, hook and basal body. the basal body is made up of a rod and several ring structures. this study investigates the structure of the t ring which is a unique component of the v. alginolyticus sodium ion-driven flagellar basal body. using zernike phase contrast (zpc) cryo-electron tomography, we compared the 3d structures of purified hook-basal bodies (hbb) from a wild-type strain (kk148) and a deletion mutant lacking mot ...201120705140
the flagellar basal body-associated protein flgt is essential for a novel ring structure in the sodium-driven vibrio vibrio alginolyticus, the flagellar motor can rotate at a remarkably high speed, ca. three to four times faster than the escherichia coli or salmonella motor. here, we found a vibrio-specific protein, flgt, in the purified flagellar basal body fraction. defects of flgt resulted in partial fla⁻ and mot⁻ phenotypes, suggesting that flgt is involved in formation of the flagellar structure and generating flagellar rotation. electron microscopic observation of the basal body of δflgt cells reveale ...201020729351
first molluscan transcription factor activator protein-1 (ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.the regulation of transcriptional activation is an essential and critical point in gene expression. in this study, we describe a novel transcription factor activator protein-1 (ap-1) gene from disk abalone haliotis discus discus (abap-1) for the first time in mollusk. it was identified by homology screening of an abalone normalized cdna library. the cloned abap-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. the abap-1 gene is composed of a charac ...201020732431
dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus.the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ...200920735646
a multidomain galectin involved in innate immune response of pearl oyster pinctada fucata.galectins could specifically bind to β-galactoside residues and play crucial roles in innate immune responses of vertebrates and invertebrates. in this study, the cdna of a galectin with multiple carbohydrate-recognition domains (crds) was cloned from pearl oyster pinctada fucata (designated as pogal). pogal cdna was 2138bp long and consisted of a 5'-untranslated region (utr) of 120bp, a 3'-utr of 350bp with two cytokine rna instability motifs (attta), and an open reading frame (orf) of 1668bp e ...201120813129
immunoglobulin m gene expression analysis of orange-spotted grouper, epinephelus coioides, following heat shock and vibrio alginolyticus challenge.the present study aimed to examine the expression of immunoglobulin m (igm) gene in orange-spotted grouper (epinephelus coioides) following thermal stress, bacterial infection, and immunization with formalin-killed vibrio alginolyticus, a kind of bacterial pathogen that causes septicemia. in heat shock experiments, twenty-five healthy orange-spotted grouper were kept in tanks with seawater at 37±0.5°c for one hour heat-shock treatment, and then returned to 27±0.5°c seawater tanks. in bacterial c ...201020816805
litopenaeus vannamei tumor necrosis factor receptor-associated factor 6 (traf6) responds to vibrio alginolyticus and white spot syndrome virus (wssv) infection and activates antimicrobial peptide genes.tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a key signaling adaptor protein not only for the tnfr superfamily but also for the interleukin-1 receptor/toll-like receptor (il-1/tlr) superfamily. to investigate traf6 function in invertebrate innate immune responses, litopenaeus vannamei traf6 (lvtraf6) was identified and characterized. the full-length cdna of lvtraf6 is 2823bp long, with an open reading frame (orf) encoding a putative protein of 594 amino acids, including a ...201120816892
crystal structure and mutational analysis of aminoacylhistidine dipeptidase from vibrio alginolyticus reveal a new architecture of m20 metallopeptidases.aminoacylhistidine dipeptidases (pepd, ec belong to the family of m20 metallopeptidases from the metallopeptidase h clan that catalyze a broad range of dipeptide and tripeptide substrates, including l-carnosine and l-homocarnosine. homocarnosine has been suggested as a precursor for the neurotransmitter γ-aminobutyric acid (gaba) and may mediate the antiseizure effects of gabaergic therapies. here, we report the crystal structure of pepd from vibrio alginolyticus and the results of mut ...201020819954
molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ...201120848212
cloning and characterization of two ferritin subunit genes from bay scallop, argopecten irradians (lamarck 1819).we have cloned two full-length cdnas from two ferritin genes (aifer1 and aifer2) of the bay scallop, argopecten irradians (lamarck 1819). the cdnas are 1,019 and 827 bp in length and encode proteins of 171 and 173 amino acids, respectively. the 5' utr of each contains a conserved iron response element (ire) motif. sequence analyses reveal that both proteins belong to the h-ferritin family with seven conserved amino acids in the ferroxidase center. highest expression of aifer1 is found in the man ...201020878240
diet enriched with mushroom phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, epinephelus bruneus against vibriosis.the effect of diet supplemented with phellinus linteus fed for 30 days was investigated in grouper epinephelus bruneus challenged with vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, and vibrio carchariae; infected and treated fish had a significantly higher percent weight gain and feed efficiency. in groups fed with enriched diet and challenged with v. anguillarum and v. harveyi the mortality rate declined with a consequent rise in survival rate than with other pathogens. on the other ...201020883799
an acute gastroenteritis outbreak of vibrio parahaemolyticus o4:k55 in nursing college, thailand.a cluster of acute gastroenteritis among nursing students was noticed on 13th september 2005. between 13th and 17th september 2005, a retrospective cohort study was then conducted to identify the most likely cause of gastroenteritis at a nursing college in bangkok, thailand. self-administered questionnaires, interviews, environmental investigations, and rectal swabs from all participants were carried out. in the investigation, 98.9% female nursing students were investigated and had completed the ...201020962724
transcriptional regulation of extracellular copper zinc superoxide dismutase from white shrimp litopenaeus vannamei following vibrio alginolyticus and wssv infection.the cdna encoding an extracellular copper zinc superoxide dismutase (lvecsod) was cloned from the hepatopancreas of white shrimp litopenaeus vannamei. it consisted of 915 bp nucleotides with an open reading frame corresponding to a deduced protein of 178 amino acids. the lvecsod contains a putative signal peptide of 16 amino acids, two potential n-linked glycosylation sites (n(115)gta and n(135)itg) and a copper zinc superoxide dismutase family signature sequence (g(162)nagarvaccti(173)). it was ...201020974259
identification of outer membrane proteins of vibrio parahaemolyticus and vibrio alginolyticus altered in response to γ-irradiation or long-term starvation.vibrio parahaemolyticus and vibrio alginolyticus were subjected to γ-irradiation (0.5 kgy) or starvation by incubation for 8 months in seawater to study modifications in their outer membrane protein patterns. after treatment, outer membrane protein profiles of starved or γ-irradiated bacteria were found to be altered when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). altered proteins were identified by mass spectrometry (ms and ms/ms) and analyses revealed tha ...201021035543
autophagy is induced by the type iii secretion system of vibrio alginolyticus in several mammalian cell lines.vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in marine animals as well as humans. here, we further characterized a cell death mechanism caused by this bacterium in several mammalian cell lines. the t3ss of v. alginolyticus killed hela cells by a very similar cell cytolysis mechanism in fish cells, as evidenced by cell rounding and ldh release; however, dna fragmentation was not observed. further studies showed that caspase-1 and caspase-3 ...201021046072
galectin-1 (ofglec-1) from rock bream (oplegnathus fasciatus): identification, characterization, gene expression and functional properties.lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this study, a novel galectin-1 (designated ofglec-1) gene was identified from rock bream, oplegnathus fasciatus, cdna library. the complete cdna sequence of the ofglec-1 gene is 993 base pairs in length, and encodes 135 amino acids. ofglec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. the mrna expressions of ofglec-1 in healthy and bacteri ...201021073891
a polyphasic approach for the differentiation of environmental vibrio isolates from temperate waters.climate change and marine traffic lead to changing species communities in the oceans. due to increasing seawater temperatures, pathogenic vibrio species could become significant even in temperate waters. we classified mesophilic vibrio isolates from the german bight (north sea) using a polyphasic approach with special emphasis on vibrio parahaemolyticus. matrix-assisted laser desorption/ionization time-of-flight ms was used as a primary screen to classify isolates, 16s rrna gene and rpob gene se ...201021118277
molluscan death effector domain (ded)-containing caspase-8 gene from disk abalone (haliotis discus discus): molecular characterization and expression analysis.the caspase family represents aspartate-specific cysteine proteases that play key roles in apoptosis and immune signaling. in this study, we cloned the first death effector domain (ded)-containing molluscan caspase-8 gene from disk abalone (haliotis discus discus), which is named as hdcaspase-8. the full-length hdcaspase was 2855 bp, with a 1908 bp open reading frame encoding 636 amino acids. the hdcaspase-8 had 72 kda predicted molecular mass with an estimated isoelectric point (pi) of 6.0. the ...201021130887
development of a single base extension-tag microarray for the detection of pathogenic vibrio species in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ...201021161212
two flagellar stators and their roles in motility and virulence in pseudomonas syringae pv. tabaci 6605.the motor proteins around the flagellar basal body consist of two cytoplasmic membrane proteins, mota and motb, and function as a complex that acts as the stator to generate the torque that drives rotation. genome analysis of several pseudomonas syringae pathovars revealed that there are two sets of genes encoding motor proteins: motab and motcd. deduced amino acid sequences for mota/b and motc/d showed homologies to the h(+)-driven stator from escherichia coli and na(+)-driven stator from vibri ...201021165649
identification of a luxo-regulated extracellular protein pep and its roles in motility in vibrio alginolyticus.the fish pathogen vibrio alginolyticus virulence factors and regulation mechanism are barely known except the quorum sensing regulated extracellular products. in the present study, the cleavable isotope-coded affinity tags (cicats) were used to analyze the differentially expressed extracellular proteins of v. alginolyticus wild-type and a δluxo mutant, and a putative peptidase pep was identified. quantitative real-time reverse transcription pcr confirmed that the transcription of pep was down-re ...201021167274
three isoforms of anti-lipopolysaccharide factor identified from eyestalk cdna library of swimming crab portunus trituberculatus.anti-lipopolysaccharide factors (alfs), as the potent antimicrobial peptides, can bind and neutralize lipopolysaccharide (lps) and exhibit broad spectrum antimicrobial activities. in this study, three isoforms of the alf homologues (ptesalf1-3) were identified from eyestalk cdna library of swimming crab portunus trituberculatus. the full-length cdna sequences of ptesalf1, 2 and 3 were 1138, 1052 and 1057 bp encoding 92, 108 and 123 amino acids, respectively. ptesalf1-3 contained two conserved cy ...201021168510
functional characterization of vibrio alginolyticus twin-arginine translocation system: its roles in biofilm formation, extracellular protease activity, and virulence towards fish.the bacterial twin-arginine translocation (tat) system contributes to translocate folded proteins and plays pleiotropic roles in growth, motility, and the secretion of some virulent factors. in this study, the authors identified the tat gene cluster in fish pathogen vibrio alginolyticus and explored its roles in pathogenesis toward fish. vibrio alginolyticus tat mutants showed growth deficiency in tmao medium, while the complement strain restored the ability to grow in the medium, demonstrating ...201021170534
[cloning and expression pattern analysis of a lipopolysaccharide -and beta-1,3-glucan-binding protein in portunus trituberculatus].the lipopolysaccharide -and beta-1,3-glucan-binding protein (lgbp) is a pattern recognition receptor, which is fundamental for the innate immune response of crustaceans. a lgbp gene was cloned from the haemocytes of portunus trituberculatus using smart race methods. the full-length lgbp cdna (1 378 bp) had a 1 095 bp open reading frame encoding a protein of 365 amino acid residues including a 16 amino acid residues signal peptide, a 138 bp 5' untranslated region (utr) and a 144 bp untranslated r ...201021174347
differential expression of lvhsp60 in shrimp in response to environmental stress.previous studies showed that heat-shock protein 60 (hsp60) was known to function as a molecular chaperone and is an important factor in the innate immune system in mammals. however, little was known about the physiological relevance of hsp60 in marine invertebrates. this study focuses on long-term monitoring of the differential expression of lvhsp60 in shrimp litopenaeus vannamei in response to environmental stress. the thermal aggregation assay elucidated that lvhsp60 was an effective chaperone ...201021182965
implications of three-step swimming patterns in bacterial chemotaxis.we recently found that marine bacteria vibrio alginolyticus execute a cyclic three-step (run-reverse-flick) motility pattern that is distinctively different from the two-step (run-tumble) pattern of escherichia coli. how this novel, to our knowledge, swimming pattern is regulated by cells of v. alginolyticus is not currently known, but its significance for bacterial chemotaxis is self-evident and will be delineated herein. using a statistical approach, we calculated the migration speed of a cell ...201121190654
f-type lectin involved in defense against bacterial infection in the pearl oyster (pinctada martensii).in invertebrates and vertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. in the present study, we report the cloning of an f-type lectin (designated as pmf-lectin) from pearl oyster (pinctada martensii) using a combination of expression sequence tag (est) analysis and rapid amplification of cdna ends (race) pcr. the full-length cdna of pmf-lectin contains an open reading frame (orf) of 579 bp coding for192 amino acids. the de ...201021195768
protection of red snapper (lutjanus sanguineus) against vibrio alginolyticus with a dna vaccine containing flagellin flaa gene.the main aims of this study were to construct a dna vaccine containing flagellin flaa gene from vibrio alginolyticus strain hy9901 and to explore the potential application of pcdna-flaa as a dna vaccine candidate for red snapper (lutjanus sanguineus).201121198695
conversion of mono-polar to peritrichous flagellation in vibrio alginolyticus.precise regulation of the number and positioning of flagella are critical in order for the mono-polar-flagellated bacterium vibrio alginolyticus to swim efficiently. it has been shown that, in v. alginolyticus cells, the putative gtpase flhf determines the polar location and production of flagella, while the putative atpase flhg interacts with flhf, preventing it from localizing at the pole, and thus negatively regulating the flagellar number. in fact, no δflhf cells have flagella, while a very ...201121204943
from the cover: bacterial flagellum as a propeller and as a rudder for efficient chemotaxis.we investigate swimming and chemotactic behaviors of the polarly flagellated marine bacteria vibrio alginolyticus in an aqueous medium. our observations show that v. alginolyticus execute a cyclic, three-step (forward, reverse, and flick) swimming pattern that is distinctively different from the run-tumble pattern adopted by escherichia coli. specifically, the bacterium backtracks its forward swimming path when the motor reverses. however, upon resuming forward swimming, the flagellum flicks and ...201121205908
phylogenetic and evolutionary analysis of vibrio parahaemolyticus and vibrio alginolyticus isolates based on toxr gene sequence.the vibrio genus contains a large number of closely related bacterial species differing, in some cases, less than 1% in 16s rrna gene sequence. the present study evaluated the usefulness of toxr gene for phylogenetic and evolution analysis on vibrio isolates of environmental or clinical origin belonging to the two closely related species v. parahaemolyticus and v. alginolyticus. the phylogenetic analysis based on toxr gene, contrary to 16s rrna gene, allowed a clear differentiation of the isolat ...201021213595
microarray analysis of gene expression in disk abalone haliotis discus discus after bacterial this study, we investigated the gene expression profiling of disk abalone, haliotis discus discus challenged by a mixture of three pathogenic bacteria vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes using a cdna microarray. upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels ≥2 or ≤2 -fold in gills and digestive tract, respectively. there were 46 tissue-specific transcripts that up-regulated specifically in the digestive ...201121215803
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