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[vibrio alginolyticus and swimmer's otitis externa. 2 cases and review of the literature].we describe two patients with acute diffuse external otitis (swimmer's otitis) acquired in the mediterranean shore, with vibrio alginolyticus recovered from ear fluid. we describe the biochemical profile and sensitivity pattern (mic) of both strains, comparing it to previously published data. a literature review was also performed, in which we found evidence for increasing concern of v. alginolyticus as an human pathogen. also there is a need for considering halophilic vibrio as potential pathog ...19911822155
[identification of species of microorganisms of the genus vibrio].during 1988, a study was made on 61 microorganisms, genus vibrio, which were received at the national reference laboratory for acute diarrheic diseases. pedro kouri institute of tropical medicine. of them, 46 were from children with acute diarrheic disease and 15 were isolated from the environment. by means of biochemical tests. 61 vibrio cholerae no. 01. 9 vibrio parahaemolyticus and 1 vibrio alginolyticus were identified. emphasis is placed upon the importance of keeping a systematic surveilla ...19911812527
molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria.sucrose-positive derivatives of escherichia coli k-12, containing the plasmid pur400, and of klebsiella pneumoniae hydrolyse intracellular sucrose 6-phosphate by means of an invertase into d-glucose 6-phosphate and free d-fructose. the latter is phosphorylated by an atp-dependent fructokinase (gene scrk of an scr regulon) to d-fructose 6-phosphate. the lack of scrk does not cause any visible phenotype in wild-type strains of both organisms. using genes and enzymes normally involved in d-arabinit ...19911809835
two generalized transducing phages in vibrio parahaemolyticus and vibrio alginolyticus.two bacteriophages named phi vp253 and phi vp143 isolated after ultraviolet induction from lysogenic strains of vibrio parahaemolyticus have been shown to be generalized transducing phages. so far, seven different auxotrophic markers of a v. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2 x 10(-7) to 7.5 x 10(-5) per infected cell at the m.o.i. of approximately 1.0. the phage phi vp143, but not phi vp253, lysed 20 of the 28 strains of v. alginolyticus and the occu ...19911808461
acute intoxication as a cause of wild bird mortality in the netherlands from 1975-1989. 20071805755
inbreeding in saguenay-lac-st-jean (quebec, canada): a study of catholic church dispensations 1842-1971.saguenay-lac-st-jean (slsj) is a rather geographically isolated region of quebec which shows a high occurrence of hereditary disorders. it has been suggested that high inbreeding might explain this situation. we studied the inbreeding in the slsj region by 10-year periods from 1842 till 1971 using the catholic church dispensations. the values of the mean inbreeding coefficient were found to be low during the whole period, reaching a peak of 22.94 x 10(-4) during the period 1902-1911. the values ...20071797631
synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues.the nucleosides n6-methoxy-2'-deoxyadenosine (dz) and 2-amino-9-(2-deoxy-beta-ribofuranosyl)-6-methoxyaminopurine (dk) have been synthesised and converted into 5'-o-dimethoxytrityl 3'-(2-cyanoethyl n,n-diisopropylphosphoramidites). these monomers have been used in machine dna synthesis to give a set of heptadecanucleotides containing up to three analogue nucleotides. the melting transitions (tm) show that the 17-mer duplexes containing z.t and z.c base-pairs have closely similar stabilities, as ...20061797376
synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues.the nucleosides n6-methoxy-2'-deoxyadenosine (dz) and 2-amino-9-(2-deoxy-beta-ribofuranosyl)-6-methoxyaminopurine (dk) have been synthesised and converted into 5'-o-dimethoxytrityl 3'-(2-cyanoethyl n,n-diisopropylphosphoramidites). these monomers have been used in machine dna synthesis to give a set of heptadecanucleotides containing up to three analogue nucleotides. the melting transitions (tm) show that the 17-mer duplexes containing z.t and z.c base-pairs have closely similar stabilities, as ...20061797376
measurement of red blood cell antibodies in autoimmune hemolytic anemia.the enzyme-linked antiglobulin test (elat) was employed to measure the number of igg molecules per red blood cell (igg/rbc) in 11 patients with autoimmune hemolytic anemia (aiha). all patients with aiha had high levels of red cell-associated igg (110-3, 650 igg/rbc). the control group consisted of normal volunteers (n = 10) and patients with hereditary spherocytosis (n = 1), beta 0-thalassemia (n = 1), immunologic thrombocytopenic purpura (n = 3) and igg multiple myeloma (n = 4). all control ind ...20061797282
measurement of red blood cell antibodies in autoimmune hemolytic anemia.the enzyme-linked antiglobulin test (elat) was employed to measure the number of igg molecules per red blood cell (igg/rbc) in 11 patients with autoimmune hemolytic anemia (aiha). all patients with aiha had high levels of red cell-associated igg (110-3, 650 igg/rbc). the control group consisted of normal volunteers (n = 10) and patients with hereditary spherocytosis (n = 1), beta 0-thalassemia (n = 1), immunologic thrombocytopenic purpura (n = 3) and igg multiple myeloma (n = 4). all control ind ...20061797282
[paraneoplastic syndromes in urology].the paraneoplastic syndromes in patients with malignant urological tumors are reviewed herein. some general aspects of these syndromes are briefly described and discussed. similarly, the different urological tumors in which paraneoplastic syndromes have been observed are briefly reviewed. these are very common in renal cancer and cancer of the prostate, uncommon in bladder cancer and rare in testicular cancer.20071796856
isolation of vibrio alginolyticus from blood cultures in a leukaemic patient after consumption of oysters. 19911794376
the sodiummotive nadh-ubiquinone oxidoreductase of vibrio alginolyticus. 19911783108
colorectal neoplasia: screening average risk individuals. 20061782367
colorectal neoplasia: screening average risk individuals. 20061782367
vibrio alginolyticus in the u.k. 19911753130
ion channel expression by white matter glia: the type-1 astrocyte.we describe the electrophysiological properties of acutely isolated type-1 astrocytes using a new "tissue print" dissociation procedure. because the enzymes used did not destroy or modify the ion channels, and the cells retained many processes, the properties may reflect those in vivo. the types of ion channels in type-1 astrocytes changed rapidly during the first 10 postnatal days, when they attained their adult phenotype. this change was dependent on the presence of neurons. in culture, most o ...20061698397
[excitation-contraction coupling and ion transport in membrane of skeletal muscle]. 20061698230
bone marrow involvement by non-hodgkin's lymphoma: the clinical significance of morphologic discordance between the lymph node and bone marrow. nebraska lymphoma study group.bone marrow specimens from 317 patients with non-hodgkin's lymphoma (nhl) obtained at initial staging were evaluated for the presence of lymphoma or benign lymphoid aggregates. thirty-two percent (102 patients) had lymphoma in their bone marrow, and 9% had benign lymphoid aggregates. bone marrow lymphoma was present in 39% of low-grade, 36% of intermediate-grade, and 18% of high-grade lymphomas. the bone marrow was involved in 25% of patients with diffuse large-cell or immunoblastic nhl (ie, dif ...20061694234
na(+)-coupled alternative to h(+)-coupled primary transport systems in bacteria.protons are the most common coupling ions in bacterial energy conversions. however, while many organisms, such as the alkaliphilic bacilli, employ h(+)-bioenergetics for electron transport phosphorylation, they use na+ as the coupling ion for transport and flagellar movement. the na+ gradient required for these bioenergetic functions is established by the secondary na+/h+ antiporter. in contrast, vibrio alginolyticus and methanogenic bacteria have primary pumps for both h+ and na+. they use the ...19911665692
regulation of the mouse mammary tumor virus (mmtv) binding site in cultured mammary tissue.the mouse mammary tumor virus (mmtv) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. the hormonal regulation of this protein was studied both in vitro and in vivo. in mammary gland explant culture, levels of the mmtv binding protein were maintained only when prolactin was present in the medium. the further addition of progesterone induced levels to 164% of controls, while estradiol was without effect. these results were reproduce ...19911665450
f0f1-atpase from vibrio alginolyticus. subunit composition and proton pumping activity.an f0f1-atpase was isolated from the membranes of the marine bacterium vibrio alginolyticus. homology between the subunits of the f0-complexes from e. coli and v. alginolyticus was found using antibodies against subunits a, b and c of the e. coli f0f1-atpase. the f0f1-complex from v. alginolyticus was reconstituted into proteoliposomes, which were competent in atp-dependent proton uptake. this process was inhibited by triphenyltin, dccd, and venturicidin. na+ did not affect proton translocation.19911647986
[cornea bank: an increase in the number of transplantations is possible]. 20061636297
differentiated thyroid carcinomas: the relevance of various pathological features for tumour classification and prediction of tumour progress.in this series of 263 surgically treated cases of thyroid cancer, 12% were finally classified as benign lesions after histopathological review. difficulty in the assessment of tumour capsule invasion in follicular neoplasms was the most frequent cause of diagnostic error. squamous metaplasia, clusters of ground glass nuclei and psammoma bodies were found to be the most specific discriminators between papillary and follicular carcinomas. among papillary carcinomas, tumour diameter above 30 mm, th ...20061636246
characteristic differences in the mode of quinone reduction and stability between energy-coupled and -uncoupled nadh-quinone reductases from bacterial respiratory chain.bacterial respiratory chain has two types of nadh-quinone reductase (nqr): one is energy-coupled (type-1) and the other had no energy-transducing capacity, that is, energy-uncoupled (type-2). each of the nadh-reacting flavoprotein subunits of nqr-1 from escherichia coli and the marine vibrio alginolyticus reduced quinone to semiquinone radicals by the one-electron transfer pathway and was very sensitive to preincubation with nadh. on the other hand, the nqr-2 from these bacteria reduced quinone ...19921628743
diary from a week in practice. 20061595508
the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes.chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ...19921594593
[changes in centrally released arginine vasopressin by stimulation of the medullary reticular formation].it has been reported that after 40 minutes of stimulation of the medullary reticular formation (morf), widespread significant increase by 1.4% to 2.8% in brain water content occurs in white matter of the injured hemisphere. recent studies indicate that centrally released arginine vasopressin (avp) influences water permeability of the brain in both normal and pathological conditions. the present study was carried out to clarify the effect of electrical stimulation of morf on centrally released av ...19921562384
properties of respiratory chain-linked na(+)-independent nadh-quinone reductase in a marine vibrio alginolyticus.the respiratory chain of a marine vibrio alginolyticus contains two types of nadh-quinone reductase (nqr): one is an na(+)-dependent nqr functioning as an na+ pump (nqr-1) and the other is an na(+)-independent nqr (nqr-2). nqr-2 was purified about 55-fold from the membrane of mutant nap-1 which is devoid of nqr-1, and its properties were compared with those of nqr-1. in contrast to nqr-1, the purified nqr-2 does not require any salts for activity and is not inhibited by up to 0.4 m salts. the op ...19921543699
hepatitis update. the delta virus. 20061539959
case report: myocardial ischemia: an overlooked substrate in syncope of aortic stenosis.the cause of the syncope in aortic stenosis has been the subject of controversy partly because only a few patients have been monitored during their syncopal episodes. among the mechanisms proposed are hypersensitive carotid sinus, complete a-v block, ventricular arrhythmias, and ischemic myocardial depression. it is now accepted that the syncope is caused by a vasodepressor response from stimulation of left ventricular baroceptors, resulting in reflex hypotension and bradycardia. this case repor ...20061539605
effects of celiprolol on cardiovascular reactivity to laboratory stressors in patients with hypertension.an ideal antihypertensive drug should reduce blood pressure not only at rest but also during stressful situations. to test whether this criterion is fulfilled by celiprolol, a new beta 1-selective adrenergic blocker drug with peripheral vasodilating activity, we examined the effects of its oral administration on cardiovascular reactivity to laboratory stressors in 18 patients with essential hypertension, according to a placebo-controlled crossover design. in the 12 patients classified as "respon ...20061534042
interleukin-1 receptor antagonist protein inhibits interleukin-8 expression in lipopolysaccharide-stimulated human whole blood.interleukin-8 (il-8) is a neutrophil and lymphocyte chemoattractant and activator that may play an important role in mediating events at sites of inflammation. il-8 is produced by many cell types in response to a variety of inducers, including interleukin-1 (il-1). studies were conducted to address the question of whether an inhibitor of il-1 action, il-1 receptor antagonist protein (irap), would suppress il-8 production. lipopolysaccharide (lps)-stimulated human whole blood was used as an ex vi ...19921533750
purification and characterization of l-2,4-diaminobutyrate decarboxylase from acinetobacter calcoaceticus.acinetobacter calcoaceticus atcc 23055 produces a large amount of 1,3-diaminopropane under normal growth conditions. the enzyme responsible, l-2,4-diaminobutyrate (daba) decarboxylase (ec 4.1.1.-), was purified to electrophoretic homogeneity from this bacterium. the native enzyme had an m(r) of approximately 108,000, with a pi of 5.0, and was a dimer composed of identical or nearly identical subunits with apparent m(r) 53,000. the enzyme showed hyperbolic kinetics with a km of 1.59 mm for daba a ...19921512577
fatal outcome with use of rectal morphine for postoperative pain control in an infant. 20061489382
development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans, fish, and shellfish.monoclonal antibodies (mabs) against vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. the pathogens included vibrio alginolyticus, v. anguillarum, v. carchariae, v. cholerae, v. damsela, v. furnissii, v. harveyi, v. ordalii, v. parahaemolyticus, and v. vulnificus. three types of mabs were selected. the first important group included mabs that reacted with only a single species. a second group comprised a number of mabs that reacted w ...19921482190
vibrio alginolyticus ("achromobacter") collagenase: biosynthesis, function and application.bacterial collagenase from aerobic non-pathogenic vibrio alginolyticus chemovar iophagus ("achromobacter" collagenase, ec 3.4.24.08) is an inducible extracellular metallo-proteinase. production of vibrio collagenase is induced specifically by collagen or by its macromolecular fragments. on the cell surface is expressed a specific receptor recognizing collagen structure. the study of natural inducers led to synthetic peptides with inducing properties. vibrio collagenase cleaves collagen helical c ...19921480012
ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride.intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ...19921479187
diagnosis and management of the dandy-walker malformation: 30 years of experience.thirty-seven patients with dandy-walker malformation were treated between 1959 and 1989. eighty percent of patients became symptomatic by 3 years of age, with 70% presenting within the 1st year of life. hydrocephalus was present in 91% of patients at the time of diagnosis. clinical presentation was most often heralded by symptoms and signs of hydrocephalus with focal neurological findings being a less prominent feature. one third of children demonstrated developmental delay. associated congenita ...20061472430
amiloride at ph 7.0 inhibits the na(+)-driven flagellar motors of vibrio alginolyticus but allows cell growth.amiloride, a specific inhibitor for the na(+)-driven flagellar motors of alkalophilic bacillus, is known to inhibit secondarily the growth of alkalophiles. the motility of a marine vibrio, v. alginolyticus, was almost completely inhibited by 2 mm amiloride either at ph 7.0 or 8.5. we found that this concentration of amiloride inhibited the cell growth completely at ph 8.5 but only slightly at ph 7.0. kinetic analysis of the inhibition of motility by amiloride at ph 7.0 showed that the inhibition ...19921459240
[membrane atpase of vibrio alginolyticus. ion transport activity and homology with f0f1-atpase from e. coli].f0f1-atpase has been isolated from the marine alkali-resistant bacterium vibrio alginolyticus. the enzyme subunits cross-reacted with antibodies against subunits alpha, beta, gamma, epsilon, and b of e. coli atpase. the purified atpase was reconstituted into liposomes effecting an atp-dependent uptake of h+. proton transport was inhibited by the atpase blockers dccd, triphenyltin, and venturicidin. na+ ions had no effect on atp-dependent proton transport. no atp-dependent transport of na+ was de ...19921457596
[vibrio alginolyticus in clinical specimens]. 19921450271
fine needle aspiration cytology of primary soft tissue tumors. morphologic analysis of the most frequent types.we reviewed 98 cases of fine needle aspiration of soft tissue tumors with histologic confirmation performed during an eight-year period and propose a working morphologic classification based on the most prominent cytologic features. six main tumor groups are recognized: myxoid, round cells, spindle cells, pleomorphic cells, polygonal cells and well differentiated. we believe that this classification allows recognition of the most common soft tissue tumors while helping with the differential diag ...20061449029
how can the optometric profession expand the pool of qualified applicants?the health professions, including optometry, are facing a declining applicant pool. the american optometric association has taken steps to address the challenge of increasing the pool of qualified applicants to the schools and colleges of optometry. however, the most significant factor in the process will be the efforts of individual aoa members.20061447468
prediction of transmembrane topology of f0 proteins from escherichia coli f1f0 atp synthase using variational and hydrophobic moment analyses.the a subunit, a membrane protein from the e. coli f1f0 atp synthase has been examined by fourier analysis of hydrophobicity and of amino-acid residue variation. the amino-acid sequences of homologous subunits from vibrio alginolyticus, saccharomyces cerevisiae, neurospora crassa, aspergillus nidulans, schizosaccharomyces pombe and candida parapsilosis were used in the variability analysis. by fourier analysis of sequence variation, two transmembrane helices are predicted to have one face in con ...19921445940
comparative study of magnetic resonance and ct scan imaging in cases of severe head injury.the distribution, frequency, and appearance of head injuries were evaluated with mri and ct in a prospective study of 155 patients with acute (n = 124) and chronic (n = 31) head injuries. mri was significantly more sensitive than ct in the detection of intraaxial injury at any stage. in severe cases, central structure lesions were detected in approximately 80% of patients. severity on admission was compatible with mr findings. however it was difficult to decide on neurobehavioural prognosis from ...20061414552
characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins.irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ...19921406279
incidence of toxigenic vibrios in foods available in taiwan.a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ...19921399913
the measurement of radiant temperature in neonatal thermal environments.heat exchange through radiation is recognized as the dominant mode of heat transfer for an infant nursed in an incubator or warmer. a radiometer was constructed to measure the planar radiant temperature experienced by the infant. two heat-flow sensors of different emissivities were mounted onto a heat sink such that one measured principally convective heat exchange while the other measured convective and radiant heat exchange. the radiant heat exchange was obtained from the difference between th ...20061393209
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
mapping of antigenic sites to monoclonal antibodies on the primary structure of the f1-atpase beta subunit from escherichia coli: concealed amino-terminal region of the subunit in the f1.to analyze relationships between the ternary and primary structures of the beta subunit of escherichia coli f1 atpase, we prepared two monoclonal antibodies beta 12 and beta 31 against the beta peptide. these antibodies bind to the beta subunit but do not bind to the f1 atpase, resulting in no inhibition of the atpase activities. several different portions of the beta subunit peptide were prepared by constructing expression plasmids carrying the corresponding dna segment of the beta subunit gene ...19921373594
expression of bacillus amyloliquefaciens amylase and vibrio alginolyticus protease a fusion genes.previously we reported [deane, s. m., maharaj, r., robb, f. t. & woods, d. r. (1987) journal of general microbiology 133, 2295-2302] that the production of a vibrio alginolyticus sds-resistant alkaline serine protease (pro a) cloned in escherichia coli was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active pro a. replacement of the v. alginolyticus promoter region by the alpha-amylase promoter region from bacillus amyloliquefaciens resulted in th ...19921373436
variations in rrna content of marine vibrio spp. during starvation-survival and recovery.the degree and temporal context of variations in ribosome content during nutrient starvation of two copiotrophic marine bacteria, vibrio alginolyticus and vibrio furnissii, have been examined. the organisms were starved either by nutritional shift-down or by consumption of limiting nutrients resulting from growth into stationary phase. measurements of the amount of hybridization to 16s rrna-specific probes revealed that the cells retained between 10 and 26% of their original rrna content after 1 ...19921371659
towards a phylogeny of the genus vibrio based on 16s rrna sequences.the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ...19921371064
biotechnology in japan--towards the year 2000 ... and beyond. 20061367243
serum-free media and cell cultures. introductory remarks. 20051367047
serum-free media and cell cultures. introductory remarks. 20051367047
diffusion in immobilized-cell gels.the relationship between the diffusion coefficient, the effective diffusion coefficient and the partition coefficient for a solute in a cell-containing gel is discussed. the use of correlation equations that are based on some kind of physical model is recommended when the effect of cell concentration on diffusion is interpreted.20051367033
diffusion in immobilized-cell gels.the relationship between the diffusion coefficient, the effective diffusion coefficient and the partition coefficient for a solute in a cell-containing gel is discussed. the use of correlation equations that are based on some kind of physical model is recommended when the effect of cell concentration on diffusion is interpreted.20051367033
practical methods for preservation of cultured cells by freezing. 20051366945
[h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors].among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ...19921347325
fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection.thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ...19921347287
catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus.cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ...19921328162
roles of k+ and na+ in ph homeostasis and growth of the marine bacterium vibrio alginolyticus.the marine bacterium vibrio alginolyticus, containing 470 mm-k+ and 70 mm-na+ inside its cells, was able to regulate the cytoplasmic ph (ph(in)) in the narrow range 7.6-7.8 over the external ph (ph(out)) range 6.0-9.0 in the presence of 400 mm-na+ and 10 mm-k+. in the absence of external k+, however, phin was regulated only at alkaline ph(out) values above 7.6. when the cells were incubated in the presence of unusually high k+ (400 mm) and 4 mm na+, the ph(in) was regulated only at acidic ph(out ...19921326594
structural gene and complete amino acid sequence of vibrio alginolyticus collagenase.the dna encoding the collagenase of vibrio alginolyticus was cloned, and its complete nucleotide sequence was determined. when the cloned gene was ligated to puc18, the escherichia coli expression vector, bacteria carrying the gene exhibited both collagenase antigen and collagenase activity. the open reading frame from the atg initiation codon was 2442 bp in length for the collagenase structural gene. the amino acid sequence, deduced from the nucleotide sequence, revealed that the mature collage ...19921311172
wheezy bronchitis in children; etiopathogenetic role of non-infectious agents. 20051305512
the isolation of vibrio parahaemolyticus and related vibrios from moribund aquarium lobsters.vibrios were isolated in pure culture from the hemolymph of 7 out of 28 dead or dying aquarium lobsters which had been acclimated to 20-22 degrees c. one isolate was identified as vibrio parahaemolyticus, one as a related marine vibrio (probably v. marinus), and five as vibrio alginolyticus. no isolates of halophilic vibrio species were made from healthy lobsters using thiosulfate citrate bile salts sucrose agar (tcbs).19761260536
self extraction of foreign body lodged in subtarsal sulcus (a simple first-aid measure). 19951236449
management of pathological fractures at the mayo clinic. 20051234120
platelet monoamine oxidase activity in patients with chronic schizophrenic illnesses untreated by neuroleptic drugs [proceedings]. 20051234010
[antidepressive treatment with nortriptyline]. 20051233907
[salmonellosis carrier state]. 19751214656
common morphogenetic aspects of various organotypic microvascular patterns. 19751214601
isolation of vibrio alginolyticus from wound infections.vibrio alginolyticus was isolated from two patients with leg wounds. both had a history of recent exposure to ocean water.19751206111
lethal effect of fresh sea water on vibrio parahaemolyticus and isolation of bdellovibrio parasitic against the organism.halophilic bdellovibrio, which is parasitic and lytic to vibrio pharahaemolyticus, was ioslated from fresh sea water in the winter. it had a lethal effect on v. parahaemolyticus. the optimum temperature ofr multiplication ranged from 25 c to 30 c and growth was not observed at 35 c. plaque numbers of the isolate reached a maximum in 17 hr under conditions of shaking at 25 c in autoclaved sea water supplemented with v. parahaemolyticus cells, and were as high as ten times the number of host cells ...19751202252
[duodenal reflux and gastritis after billroth i gastric resection (author's transl)].endoscopic and biopsy studies were undertaken in 92 patients who had undergone partial gastric resection (end-to-end gastroduodenostomy, billroth i) one to seven years previously. in addition, the amound of duodeno-gastric reflux was evaluated by measuring bromsulphalein excretion in gastric juice. atrophic gastritis occurred in 36%, while superficial gastritis was noted in 49% of patients. the degree and severity of the inflammatory changes was most marked near the anastomosis. the severity of ...20051183346
proceedings: influences of the septum on the hippocampal dentate area which are unaccompanied by field potentials. 19751151852
letter: the critical coronary disease patient. 19921130748
isolation of vibrio parahaemolyticus and vibrio alginolyticus from estuarine areas of southeastern alaska.the first reported isolations of halophilic vibrios, including vibrio parahaemolyticus, from three seafood processing areas in southeastern alaska are described.19751124924
sensitivity of trichomonas vaginalis to metronidazole, tinidazole, and nifuratel in vitro.prompted by the sensitivity of trichomonads to metronidazole and nifuratel in clinical practice, a study was conducted in 1971-1972 of 63 consecutive strains of trichomonas vaginalis isolated from women with clinically refractory vaginal discharge. their susceptibility to metronidazole, tinidazole, and nifuratel was tested, using a serial tube dilution technique. the minimum concentrations which in 48 hrs caused immobilization and lysis of trichomonads cultured in diamond's medium was assessed. ...19761087577
comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells.alkaline phosphatase [orthophosphoricmonoester phosphohydrolase (alkaline ph optimum), ec 3.1.3.1] purified from a burkitt lymphoma cell line (daudi) and moloney-virus-induced murine leukemia (yac) showed unique catalytic properties in substrate specificity and inhibition by cysteamine-s-phosphate. it migrated on polyacrylamide gel electrophoresis in a single activity band. alkaline phosphatase with similar properties was found in several human lymphoblastoid cell lines, in chronic lymphatic leu ...19761064015
marine vibrios associated with superficial septic lesions.three cases are reported in which a marine vibrio, vibrio alginolyticus, was isolated from superficial septic lesions. all cases had been exposed to sea-water. the possible significane of these findings and the need for further investigations are discussed.19761002833
[the dehydrogenation of butandiol by "vibrio parahaemolyticus" and other vibrios (author's transl)].the butandiol dehydrogenase was examined in 64 vibrios isolated in togo 1970/71. this enzyme was found in all of the not halophilic strains and in the species vibrio alginolyticus, whereas the species v. parahaemolyticus exhibited as well dehydrating as not dehydrating strains. the butandiol dehydrogenase could be a good marker for epidemiologic analysis in characterizing v. parahaemolyticus strains isolated from different sources, especially if no antisera are available.1976952445
vibrio alginolyticus infections in hawaii.clinical and bacteriological features of eight cases of vibrio alginolyticus infections in hawaii are presented. these isolates occurred in superficial sites, primarily related to infections caused by swimming.1977886005
[vibrio parahaemolyticus in mussels and in silt in the southern central baltic sea (author's transl)].because the previous studies of 541 fish of the southern central baltic sea area gave negative results for the presence of vibrio parahaemolyticus, 150 shellfish and 40 mud samples of the same origin were tested this time. in none of them presence of vibrio parahaemolyticus nor vibrio alginolyticus has been stated, though.1977842182
effects of some chemical factors on flagellation and swarming of vibrio alginolyticus.vibrio alginolyticus strains recently isolated from dutch coastal seawater changed flagellar organization when cultivated in the presence of certain chemical agents. on agar media with more than 4.0% (w/v) nacl the number of lateral flagella per cell decreased with increasing salt concentration. both on agar media and in broth cultures with 6.0-9.0% (w/v) nacl, cells with polar tufts of 2-4 sheathed or unsheathed flagella were frequently found. cells grown on agar media with 7.3-9.8% (w/v) na2so ...1975816251
characterization of vibrio alginolyticus (beneckea alginolytica) isolated from the fauna of arabian sea. 1978700852
properties of chitinase from vibrio alginolyticus, as assayed with the chromogenic substrate 3,4-dinitrophenyl tetra-n-acetylchitotetraoside [proceedings]. 1978669015
some interesting isolates from a diagnostic laboratory.citrobacter koseri, plesiomonas shigelloides, edwardsiella tarda, yersinia enterocolitica, alkalescens dispar, vibrio parahaemolyticus, and vibrio alginolyticus were seven interesting microorganisms isolated recently in our diagnostic laboratory.1978641196
[isolation of halophilic vibrios as possible agents of food poisoning (author's transl)].seventy-six halophilic vibrio strains were isolated from different marine sources such as coastal waters, fishes and mussels. twenty strains were identified as vibrio parahaemolyticus, 44 as vibrio alginolyticus and 12 other strains could not be classified because of their atypical biochemical activities. further investigations are necessary in order to assess if the present strains of v. parahaemolyticus can have a role in food poisoning outbreaks or human sporadic enteritis in our geographic a ...1977607885
na+-dependent activation of nadh oxidase in membrane fractions from halophilic vibrio alginolyticus and v. costicolus.membrane-bound nadh oxidase activities from slightly halophilic marine vibrio alginolyticus and moderately halophilic v. costicolus required 0.3 and 0.5 m na+, respectively, for maximum activity; other cations such as li+,k+,rb+,cs+,mg2+, and ca2+ were relatively ineffective as replacements for na+. the concentration of na+ required to give half-maximum activity with the nadh oxidase from v. alginolyticus was 82 mn. this value was reduced to 6.4 and 13.8 mm in the presence of 400 mm k+ and 10 mm ...1977591506
low-income widows and other aged singles.based on data collected in a nationwide survey in late 1973, this report describes the living conditions, health, social activity, and attitudes of unmarried older persons at low to middle income levels--that is, below $5,000 per year. although most previous research has focused on the special situation of widows, the current findings indicate that lower income widowers and divorced/separated/never-married persons generally experience many of the same problems as the widows and respond to them i ...2005545715
liver involvement in essential mixed cryoglobulinemia.twenty-one of 30 patients with essential mixed cryoglobulinemia (emc) had evidence of liver involvement. the liver disease was characterized by the absence of clinical symptoms, hepatosplenomegaly, mild elevation of enzymes, abnormal bsp retention and low albumin levels. histology, available in 12 patients, showed either chronic persistent or chronic active hepatitis or liver cirrhosis; 44% of the patients had hbsag or hbsab in sera and/or cryoglobulins, confirming the high frequency of exposure ...1979545644
[infections in immunosuppressed patients]. 2005544237
effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant. 2005544179
a flow calorimeter for assay of hormone- and metabolite-induced changes in steady-state heat production by tissue. 2005543542
a simple centrifuge column for desalting protein solutions. 2005543535
pathophysiological studies on schizophrenia with special reference to homovanillic acid concentration in cerebrospin fluid. 2005540184
simulated small intestinal volvulus in the anesthetized horse.experimental closed loop small intestinal volvulus was studied in the anesthetized horse. volvulus was simulated by ligation of the mesenterial veins to a segment of small intestine. physical signs and hemodynamic, hematologic, clinical chemical, bacteriologic and peritoneal fluid values were examined. compared to conscious horses anesthesia highly delayed and modified the clinical signs of shock (changes in mucosal colour, dehydration, decreased skin temperature, elevated pulse rate, low blood ...1979523313
vaginal epithelial changes associated with in utero des exposure.to determine the true prevalence of vaginal epithelial changes in des-exposed offspring, the national cancer institute funded the desad project, a study designed to follow up these women for 4 years and to determine the prevalence of cancer and various other abnormalities in des-exposed women. 3339 women have constituted the project study cohort as of 12/31/76. each woman in the desad project undergoes breast, pelvic and colposcopic examinations, as well as vaginal and cervical cytology. details ...2004523034
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