Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| mutatox test: a new test for monitoring environmental genotoxic agents. | in this study, yamaska river water and milli-q water and organically extracted sediment extracts were used to evaluate the sensitivity of a new genotoxicity screening test, the mutatox test. also in this study, the samples were tested for acute and chronic toxicity using the following screening test procedures: microtox, daphnia magna, ceriodaphnia reticulata and atp-tox systems. the mutatox test is based on the use of a dark mutant strain of photobacterium phosphoreum and is sensitive to chemic ... | 1990 | 15092256 |
| continuous culture of photobacterium. | the design and performance characteristics of a small volume (20 ml) continuous culture device for the cultivation of luminous bacteria are described. this simply constructed device can be used to supply luminescent bacteria with constant properties for either laboratory use or the assay of environmental pollutants. furthermore, bacteria can be deployed to make sensitive (<1 nm) oxygen measurements. the culture device may be configured, alone or in combination, as a chemostat, turbidostat or a " ... | 2004 | 15093217 |
| chemical speciation and toxicity of metals assessed by three bioluminescence-based assays using marine organisms. | metal toxicity is a function of the biology of the target organism and the chemical speciation of the metal. the toxicity of 11 metals was assessed with three cell-based bioassays based on marine organisms: the bacterium photobacterium phosphoreum of the microtox bioassay, an environmental strain of p. phosphoreum, and photocytes isolated from the brittlestar ophiopsila californica. metal speciation was calculated for three commonly used media: nacl-based microtox bioassay medium, artificial sea ... | 2004 | 15101032 |
| identification and characterisation of the fur genes in photobacterium damselae ssp. piscicida and ssp. damselae. | the gene encoding the ferric uptake regulator protein (fur gene) of the fish pathogenic bacterium photobacterium damselae ssp. piscicida strain d121 was partially amplified using degenerate oligonucleotides. complete sequencing of the fur gene and neighbouring dna was accomplished by primer walking. an open reading frame of 447 bp, coding for a protein of 148 amino acids, and with high homology to previously described fur proteins, was identified. the fur gene of p. damselae ssp. damselae atcc 3 ... | 2004 | 15109136 |
| activation of the nitric oxide response in gilthead seabream after experimental infection with photobacterium damselae subsp. piscicida. | inoculation of small gilthead seabream (sparus aurata) (30-75 g body weight) with a sublethal dose of different photobacterium damselae subsp. piscicida (pdp) strains (di-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (no) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. in contrast, no such response was detected in larger fish (150-600 g). since the virulence of pdp correlates with the presence of a polysaccharide capsular layer whi ... | 2004 | 15110332 |
| ion trap lc/ms characterisation of toxic polar organic pollutants in colour photographic wastewaters and monitoring of their chemical degradation. | liquid chromatography/electrospray ionisation-ion trap mass spectrometry (lc/esi-itms) with positive mode of operation was successfully applied to the characterisation of aromatic amines and chelating agents in colour photographic wastewaters. in addition to residual ingredients, monomers and dimers of sulphonated aromatic amines were the main toxic polar organic pollutants found. oxidation of wastewater components by the fenton-like reagent (fe3+ + h2o2) was investigated by continuously pumping ... | 2004 | 15116875 |
| the effect of growth medium salinity of photobacterium damselae subsp. piscicida on the immune response of hybrid bass (morone saxatilis x m. chrysops). | photobacterium damselae subsp. piscicida (p. damselae) was grown on various media and the effect of media salinity on certain immune responses of hybrid bass was studied. in israel, pasteurellosis outbreaks have not been reported at water salinities below 1.38 per thousand. during vaccination experiments the salinity of the medium on which p. damselae is grown, was shown to affect stimulation of the immune system. no correlation was found between antibody response and protection. bacterial envel ... | 2004 | 15123315 |
| use of reca as an alternative phylogenetic marker in the family vibrionaceae. | this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ... | 2004 | 15143042 |
| fatal infection of the hand due to photobacterium damsela: a case report. | we report a case of fatal acute renal failure due to photobacterium damsela infection of the hand. although the patient received intensive medical care and surgical treatment, he died 8 h after arrival at the hospital. this is the first case of p. damsela sepsis proven by blood culture in japan. | 2004 | 15156501 |
| effects of hydrogen peroxide on light emission by various strains of marine luminescent bacteria. | light-emitting bacteria are the most abundant and widespread luminescent organisms. most species of such bacteria live in marine environments. however, until recently, biological role of bacterial luminescence remained unknown. recent studies indicated that light produced in bacterial cells may stimulate dna repair. therefore, it is not surprising that agents that cause dna damage induce expression of lux genes. moreover, it was proposed previously that bacterial luciferases may be involved in d ... | 2004 | 15162391 |
| comparative toxicological evaluation of untreated and treated tannery effluent with nostoc muscorum l. (algal assay) and microtox bioassay. | the relative sensitivity of tannery effluent before and after treatment was compared by employing nostoc muscorum and microtox assay in laboratory. the effect on chlorophyll, protein and biomass content of nostoc muscorum was studied with the luminescent property inhibition of photobacterium phosphorium and compared with algal bioassay. the results of microtox assay after 5, 15 and 30 min of exposure were compared with data obtained from algal bioassay. it was observed that the luminescent prope ... | 2004 | 15195832 |
| retraction. fatal infection of the hand due to photobacterium damsela: a case report. | 2004 | 15224696 | |
| a new approach to the problem of weak magnetic fields: an effect on living objects. | 2003 | 15255202 | |
| occurrence of tetracycline resistance genes tet(m) and tet(s) in bacteria from marine aquaculture sites. | occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in japan and korea. the tet(m) gene was detected in 34 japanese and korean isolates, which included vibrio sp., lactococcus garvieae, photobacterium damsela subsp. piscicida, and unidentified gram-positive bacteria. the majority of these bacterial isolates displayed high-level resistance with a minimum ... | 2004 | 15268950 |
| [the effect of clp proteins on dnak-dependent refolding of bacterial luciferases]. | a study was made of the refolding of bacterial luciferases of vibrio fischeri, v. harveyi, photobacterium phosphoreum, and photorhabdus luminescens. by reaction rate, luciferases were divided into two groups. the reaction rate constants of fast luciferases of v. fischeri and ph. phosphoreum were about tenfold higher than those of slow luciferases of ph. luminescens and v. harveyi. the order of increasing luciferase thermostability was ph. phosphoreum, v. fischeri, v. harveyi, and ph. luminescens ... | 2004 | 15285621 |
| explorative multivariate analyses of 16s rrna gene data from microbial communities in modified-atmosphere-packed salmon and coalfish. | modified-atmosphere packaging (map) of foods in combination with low-temperature storage extends product shelf life by limiting microbial growth. we investigated the microbial biodiversity of map salmon and coalfish by using an explorative approach and analyzing both the total amounts of bacteria and the microbial group composition (both aerobic and anaerobic bacteria). real-time pcr analyses revealed a surprisingly large difference in the microbial loads for the different fish samples. the micr ... | 2004 | 15294842 |
| structure of flavoprotein fp390 from a luminescent bacterium photobacterium phosphoreum refined at 2.7 a resolution. | the three-dimensional structure of a flavoprotein, fp(390), from a luminescent bacterium, photobacterium phosphoreum, solved by the molecular-replacement method, was refined to an r factor of 24.0% for 17 433 independent reflections, from 6.0 to 2.7 a resolution, collected by synchrotron radiation. the asymmetric unit of the crystal (space group p4(3)22, a = b = 76.8 and c = 242 a) contains two monomer molecules related by a non-crystallographic twofold axis to form a dimer. there are two q-flav ... | 1996 | 15299728 |
| maternal transfer of humoral specific and non-specific immune parameters to sea bream (sparus aurata) larvae. | immunisation of sea bream (sparus aurata l.) broodstock with a novel vaccine mixture of photobacterium damsela subsp. piscicida sk7 (phdp) was performed during the period of egg development and the changes in specific and non-specific humoral immune parameters were measured. total immunoglobulin level, specific antibody titre, anti-protease activity and lysozyme activity were significantly higher in immunised parents compared to the control. after spawning significantly higher anti-protease acti ... | 2004 | 15313509 |
| mercury chloride effects on the function and cellular integrity of sea bass (dicentrarchus labrax) head kidney macrophages. | the objective of this work was to study mercury chloride effects on the function and integrity of sea bass (dicentrarchus labrax) head kidney macrophages (s-hkm), and to evaluate the response of hgcl2-exposed cells to macrophage activating factor(s) (maf) produced by sea bass head kidney leukocytes. there was considerable variability in the effects of hgcl2 on the production of reactive oxygen species (ros) by s-hkm. when incubated with hgcl2, cells from five out of nine fish tested showed a dec ... | 2004 | 15313514 |
| [influential factors on the toxicity of pentachlorophenol sodium with microtox system in water]. | the biological toxicity of pentachlorophenol sodium (na-pcp), a typical kind of aquatic organic pollutants, was tested with the microtox system using luminescent bacteria with the influence of several factors taken into consideration. the ec50 of na-pcp increases with the increasing of the ph and hardness of the sample. the ec50 (15 min) of na-pcp is approximately equal to its ec50 (20 min). compared with some common organic pollutants, which exhibit little influence on the toxicity of na-pcp wh ... | 2004 | 15327251 |
| activity of two histidine decarboxylases from photobacterium phosphoreum at different temperatures, phs, and nacl concentrations. | the major causative agent of scombroid poisoning is histamine formed by bacterial decarboxylation of histidine. the authors reported previously that histamine was exclusively formed by the psychrotrophic halophilic bacteria photobacterium phosphoreum in scombroid fish during storage at or below 10 degrees c. moreover, histamine-forming ability was affected by two histidine decarboxylases: constitutive and inducible enzymes. this article reports the effect of various growth and reaction condition ... | 2004 | 15330542 |
| random mutagenesis of bacterial luciferase: critical role of glu175 in the control of luminescence decay. | bacterial luciferases (luxab) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. luciferases from vibrio harveyi and xenorhabdus (photorhabdus) luminescens have slow decay rates, and those from the photobacterium genus, such as photobacterium fisheri, p. phosphoreum and p. leiognathi, have rapid decay rates. by substitution of a 67-amino-acid stretch of p. phosphoreum luxa in the central region of the luxa subunit, the ... | 2005 | 15352872 |
| biodiversity of vibrios. | vibrios are ubiquitous and abundant in the aquatic environment. a high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome pcr, ribotyping, and whole-genome sequencing. the ... | 2004 | 15353563 |
| phylogenetic relationships among marine alteromonas-like proteobacteria: emended description of the family alteromonadaceae and proposal of pseudoalteromonadaceae fam. nov., colwelliaceae fam. nov., shewanellaceae fam. nov., moritellaceae fam. nov., ferrimonadaceae fam. nov., idiomarinaceae fam. nov. and psychromonadaceae fam. nov. | the phylogenetic relationships among marine alteromonas-like bacteria of the genera alteromonas, pseudoalteromonas, glaciecola, thalassomonas, colwellia, idiomarina, oceanimonas, oceanisphaera, shewanella, moritella, ferrimonas, psychromonas and several other genera of the 'gammaproteobacteria' were studied. results of 16s rrna gene sequence analyses revealed that some members of these genera formed several coherent groups at the family level. characteristic signature oligonucleotides for studie ... | 2004 | 15388743 |
| a purine-requiring strain of photobacterium fischeri. | 1949 | 15393696 | |
| distinctive functional features in prokaryotic and eukaryotic cu,zn superoxide dismutases. | bacterial and eukaryotic cu,zn superoxide dismutases show remarkable differences in the active site region and in their quaternary structure organization. we report here a functional comparison between four cu,zn superoxide dismutases from gram-negative bacteria and the eukaryotic bovine enzyme. our data indicate that bacterial dimeric variants are characterized by catalytic rates higher than that of the bovine enzyme, probably due to the solvent accessibility of their active site. prokaryotic c ... | 2004 | 15449711 |
| influence of vaccination on the nitric oxide response of gilthead seabream following infection with photobacterium damselae subsp. piscicida. | the nitric oxide (no) response of vaccinated and non-vaccinated juvenile gilthead seabream was studied in vivo and the no response of isolated kidney macrophages of fish was studied in vitro. fish were vaccinated with formalin-killed photobacterium damselae subsp. piscicida (pdp) with or without freund's incomplete adjuvant (fia) and control fish received phosphate buffered saline (pbs). thirty days later, fish were injected with a sublethal dose of pdp and 3 fish/group were bled at time periods ... | 2005 | 15450966 |
| coregulation of lux genes and riboflavin genes in bioluminescent bacteria of photobacterium phosphoreum. | investigation of the expression of the riboflavin (rib) genes, which are found immediately downstream of luxg in the lux operon in photobacterium phosphoreum, provides more information relevant to the evolution of bioluminescence, as well as to the regulation of supply of flavin substrate for bacterial bioluminescence reactions. in order to answer the question of whether or not the transcriptions of lux and rib genes are integrated, a transcriptional termination assay was performed with p. phosp ... | 2004 | 15459647 |
| [effect of glucose on luciferase expression in photobacterium leiognathi]. | photobacterium leiognathi cultured in marine broth emits a luminescence that is temporarily enhanced and then extinguished by glucose. glucose reduces the luciferase level and the expression of lux abg mrna in p. leiognathi. the amount of atp in p. leiognathi is temporarily increased and then declines to the normal level. these results indicate that the extinguishing by glucose in p. leiognathi is induced by the interruption of the translation of luciferase. | 2004 | 15467278 |
| effect of dietary iron overload on photobacterium damselae ssp. piscicida pathogenicity in sea bass, dicentrarchus labrax (l.). | 2004 | 15509262 | |
| transfer of hyphomicrobium indicum to the genus photobacterium as photobacterium indicum comb. nov. | hyphomicrobium indicum johnson and weisrock 1969 lacks true budding and hyphal branching, and some phenotypic characteristics are in contrast to other true hyphomicrobia. the major quinone system (ubiquinone q-8), the g+c content of the dna (40 mol%) and the cellular fatty acid composition (16 : 0, 16 : 1 and 18 : 1 as the major components, and 12 : 0 3-oh and 14 : 0 3-oh as the hydroxy fatty acids) of h. indicum are different from the genus hyphomicrobium, but similar to the genus photobacteriu ... | 2004 | 15545443 |
| characterization of the 23s and 5s rrna genes and 23s-5s intergenic spacer region (its-2) of photobacterium damselae. | the 23s ribosomal rna (rrna) gene has been sequenced in strains of the fish pathogens photobacterium damselae subsp. damselae (atcc 33539) and subsp. piscicida (atcc 29690), showing that 3 nucleotide positions are clearly different between subspecies. in addition, the 5s rrna gene plus the intergenic spacer region between the 23s and 5s rrna genes (its-2) were amplified, cloned and sequenced for the 2 reference strains as well as the field isolates rg91 (subsp. damselae) and di21 (subsp. piscici ... | 2004 | 15584408 |
| characteristic analysis of the ampc gene encoding beta-lactamase from photobacterium phosphoreum. | the ampc gene of photobacterium phosphoreum atcc 11040 was cloned and identified. nucleotide sequence of the regulatory region r&r and the ampc gene (genbank accession no. ay787792) from p. phosphoreum has been determined, and the encoded beta-lactamase is deduced. the beta-lactamase encoded by the ampc gene has a calculated m(r) 31,198 and comprises 285 amino acid residues (pi 7.35). there is a signal peptide of 20 amino acid residues mklrfiastlllsfsqlasa to lead the beta-lactamase secretion, a ... | 2005 | 15596133 |
| genome update: proteome comparisons. | 2005 | 15632419 | |
| dark variants of luminous bacteria whole cell bioluminescent optical fiber sensor to genotoxicants. | a stable dark variant separated from photobacterium phosphoreum (a2) was fixed in agar-gel membrane and immobilized onto an exposed end of a fiber-optic linked with bioluminometer. the variant could emit a luminescent signal in the presence of genotoxic agents, such as mitomycin c (mc). the performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe thickness, bacterial cell density, and diameter of the fiber-optic core and wor ... | 2004 | 15641706 |
| photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the yellow sea in korea. | a gram-negative, motile, non-spore-forming, pleomorphic and lipolytic bacterial strain, m37t, was isolated from an intertidal sediment of the yellow sea in korea. this organism grew optimally at 25-28 degrees c and in the presence of 1-2 % nacl. it did not grow without nacl or in the presence of more than 6 % nacl. strain m37t was characterized chemotaxonomically by having q-8 as the predominant respiratory lipoquinone and c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh and c(16 : 0) as the major fat ... | 2005 | 15653897 |
| effectiveness of a divalent vaccine for sole, solea senegalensis (kaup), against vibrio harveyi and photobacterium damselae subsp. piscicida. | the protection of cultured sole, solea senegalensis, against vibrio harveyi and photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective mo ... | 2005 | 15660791 |
| effect of human serum on bioluminescence of natural and recombinant luminescent bacteria. | biphasic modification of bacterial bioluminescence by human serum was revealed: bioluminescence was inhibited at high concentrations of the serum and stimulated at low concentrations. effects of temperature and duration of exposure on bioluminescence manifested in stimulation of the inhibitory effect at higher temperature and longer exposure. the degree of inhibition of bioluminescence under in the presence of serum depends on species characteristics of the microorganism and nature of the lumine ... | 2004 | 15665923 |
| influence of hydroxypropylcyclodextrins on the toxicity of mixtures. | we studied the influence of hydroxypropylcyclodextrins (hpcds) on the toxicity of some mixtures. using the photobacterium phosphoreum toxicity test, the joint toxicological effect for mixture i (containing p-nitrobenzaldehyde and 1-nitronaphthalene) and mixture ii (containing p-nitrobenzaldehyde and malononitrile) were determined in water and in aqueous solutions of hpcds. the results indicate that, although the toxicological joint effect for mixture i (simple addition) differs from that of mixt ... | 2005 | 15667850 |
| [a primary study on antimicrobial and cytotoxic activity of marine bacteria]. | marine bacteria were isolated from marine organisms including invertebrates and seaweeds and from sea water and sediments of different sea area. ethyl acetate extractions of marine bacterial fermentation were screened for antimicrobial and cytotoxicity activities by using the methods of agar diffusion and mtt assay, and the relationship of active bacteria and their origin was studied. the results showed that 42 strains among total 341 isolated marine bacteria had antimicrobial activity, and 7 st ... | 2004 | 15669499 |
| experimental and simulative dissociation of dimeric cu,zn superoxide dismutase doubly mutated at the intersubunit surface. | the equilibrium properties of dimeric photobacterium leiognathi cu,zn superoxide dismutase mutant bearing two negative charges in the amino acid clusters at the association interface has been studied, experimentally and computationally, and compared to those of the native enzyme. pressure-dependent dissociation is observed for the mutant, as observed by the fluorescence shift of the unique tryptophan residue located at the intersubunit surface. the spectral shift occurs slowly, reaching a platea ... | 2005 | 15681652 |
| identification of fur regulated genes in the bacterial fish pathogen photobacterium damselae ssp. piscicida using the fur titration assay. | bacteria have developed a series of iron-scavenging and transport systems. the expression of many of the iron utilization genes is tightly regulated by the fe2+ loaded fur repressor protein. in this study, the fur titration assay (furta) was used to screen for dna fragments from a genomic dna library of photobacterium damselae ssp. piscicida containing potential fe2+ fur binding sites or iron binding-proteins which withdraw iron from fur. one of the clones encoded a tonb gene and adjacent a func ... | 2004 | 15689115 |
| variation in 16s-23s rrna intergenic spacer regions in photobacterium damselae: a mosaic-like structure. | phenotypically, photobacterium damselae subsp. piscicida and p. damselae subsp. damselae are easily distinguished. however, their 16s rrna gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of dna-dna similarity. the 16s-23s rrna internal transcribed spacers (its) were sequenced in two strains of photobacterium damselae subsp. piscicida and two strains of p. damselae subsp. damselae to determine the level of molecul ... | 2005 | 15691912 |
| genomic and phylogenetic characterization of luminous bacteria symbiotic with the deep-sea fish chlorophthalmus albatrossis (aulopiformes: chlorophthalmidae). | bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (aulopiformes: chlorophthalmidae) are considered to belong to the species photobacterium phosphoreum. the identification of these bacteria as p. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate between phenetically similar but evolutionarily distinct luminous bacteria. therefore, to test the species identification of chlorophthalmid symbionts, we carried out a genomotypic (repe ... | 2005 | 15691950 |
| molecular cloning and functional analysis of photobacterium damselae subsp. piscicida haem receptor gene. | a haem receptor gene from photobacterium damselae subsp. piscicida (formerly known as pasteurella piscicida) has been cloned, sequenced and analysed for its function. the gene, designated as pph, has an open reading frame consisting of 2154 bp, a predicted 718 amino acid residues and exists as a single copy. it is homologous with the haem receptors of vibrio anguillarum hupa, v. cholerae huta, v. mimicus mhua and v. vulnificus hupa at 32.7, 32.7, 45.6 and 30.9%, respectively, and is highly conse ... | 2005 | 15705153 |
| sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species. | recent studies indicated that bioluminescence of the marine bacterium vibrio harveyi may both stimulate dna repair and contribute to detoxification of deleterious oxygen derivatives. therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolutionary drives in development of luminous systems. however, experimental evidence for the physiological role of luciferase in protection of cells against oxidative stress has been d ... | 2005 | 15717160 |
| side effects in sea bass (dicentrarchus labrax l.) due to intraperitoneal vaccination against vibriosis and pasteurellosis. | sea bass (dicentrarchus labrax l.) were injected intraperitoneally with monovalent (photobacterium damselae subsp. piscicida or vibrio anguillarum) and divalent (ph. damselae subsp. piscicida and v. anguillarum) vaccine formulations, with or without adjuvants (mineral oil, liposome or alginate), to evaluate the short and long-term pathological effects. eight animals from each group were sampled one, two, four and 11 months after intraperitoneal injection. the acute peritoneal response and the pr ... | 2005 | 15722227 |
| characterization of fe/mn-superoxide dismutase from diatom thallassiosira weissflogii: cloning, expression, and property. | a cdna clone of 1114 bp encoding a putative mn-superoxide dismutase (mn-sod) from diatom thallassiosira weissflogii was cloned by the pcr technique. nucleotide sequence analysis of this cdna clone revealed that it was translated into 201 amino acid residues. when the sequence was compared with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed higher homology to mn-sod. the amino acid residues required to coordinat ... | 2005 | 15740026 |
| life at depth: photobacterium profundum genome sequence and expression analysis. | deep-sea life requires adaptation to high pressure, an extreme yet common condition given that oceans cover 70% of earth's surface and have an average depth of 3800 meters. survival at such depths requires specific adaptation but, compared with other extreme conditions, high pressure has received little attention. recently, photobacterium profundum strain ss9 has been adopted as a model for piezophily. here we report its genome sequence (6.4 megabase pairs) and transcriptome analysis. the result ... | 2005 | 15746425 |
| toxicity of agricultural dust extract using microtox. | 2005 | 15768505 | |
| photobacterium rosenbergii sp. nov. and enterovibrio coralii sp. nov., vibrios associated with coral bleaching. | six new vibrio-like isolates originating from different species of bleached and healthy corals around magnetic island (australia) were investigated using a polyphasic approach. phylogenetic analyses based on 16s rrna, reca and rpoa gene sequences split the isolates in two new groups. strains lmg 22223(t), lmg 22224, lmg 22225, lmg 22226 and lmg 22227 were phylogenetic neighbours of photobacterium leiognathi lmg 4228(t) (95.6 % 16s rrna gene sequence similarity), whereas strain lmg 22228(t) was r ... | 2005 | 15774685 |
| antimicrobial substances from rhizomes of the giant knotweed polygonum sachalinense against the fish pathogen photobacterium damselae subsp. piscicida. | the antimicrobial compounds against the fish pathogen photobacterium damselae subsp. piscicida were isolated from polygonum sachalinense rhizomes. the structures of the antimicrobial compounds 1 and 2 were determined by 1h and 13c nmr, 2d-nmr (cosy, hsqc, hmbc and roesy) and fab-ms to be phenylpropanoid glycosides, vanicoside a and b, respectively. both compounds have feruloyl and p-coumaroyl groups bonded to a sucrose moiety in their structures. vanicoside a also has an acetyl group in the sucr ... | 2005 | 15787242 |
| immobilization as a technical possibility for long-term storage of bacterial biosensors. | for applications in field experiments, the recombinant strain salmonella typhimurium ta1535 was immobilized to permit its immediate utilization after long storage periods. salmonella typhimurium ta1535 cells contain the plasmid that has an inducible sos promoter fused to a promoterless luxcdabfe operon from photobacterium leiognathi. the induction of bioluminescence occurs in the presence of the dna-damaging agent mitomycin c which stimulates the bacterial sos response. early stationary phase ce ... | 2005 | 15791471 |
| biolumbase--the database of natural and transgenic bioluminescent organisms. | the institute of biophysics sb ras hosts and maintains a specialized collection of luminous bacteria (ccibso 836) containing over 700 strains isolated in various regions of the world's oceans. the culture collection is a source of lux genes and biologically active substances. the wide application of bioluminescence in medicine and ecology has given importance to analysing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as ... | 2005 | 15803502 |
| [characterization of microbial population present in the edible seaweed, monostroma undulatum, wittrock]. | the microbiological quality of monostroma undulatum, wittrock from the southern argentinean coast, was studied for its application for human food. also the diversity and function of the native bacterial population to this green seaweed was analyzed. samples were collected in puerto deseado, province of santa cruz, southern argentina (47 degrees 45'l.s., 65 degrees 55'l.w). the samples were analyzed for the presence of psycotrophic heterotrophic bacteria, marine heterotrophic bacteria, low nutrit ... | 2004 | 15807211 |
| nonbioluminescent strains of photobacterium phosphoreum produce the cell-to-cell communication signal n-(3-hydroxyoctanoyl)homoserine lactone. | bioluminescence is a common phenotype in marine bacteria, such as vibrio and photobacterium species, and can be quorum regulated by n-acylated homoserine lactones (ahls). we extracted a molecule that induced a bacterial ahl monitor (agrobacterium tumefaciens nt1 [pzlr4]) from packed cod fillets, which spoil due to growth of photobacterium phosphoreum. interestingly, ahls were produced by 13 nonbioluminescent strains of p. phosphoreum isolated from the product. of 177 strains of p. phosphoreum (i ... | 2005 | 15812045 |
| adhesion to sole, solea senegalensis kaup, mucus of microorganisms isolated from farmed fish, and their interaction with photobacterium damselae subsp. piscicida. | abstract most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. however, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. nineteen isolates obtained from farmed senegalese sole, solea senegalensis kaup, and gilthead sea bream, sparus aurata l., have been evaluated for their cap ... | 2005 | 15813865 |
| the effect of sea bream (sparus aurata) broodstock and larval vaccination on the susceptibility by photobacterium damsela subsp. piscicida and on the humoral immune parameters. | sea bream broodstock were immunised 1 or 2 months before spawning with a novel photobacteriosis vaccine. sixty-seven-day-old larvae (mean weight 22.3 mg) originating from immunised and non-immunised parents were experimentally infected with the photobacterium damsela subsp. piscicida (phdp). larvae from immunised fish showed delayed onset and lower mortality (66.67%) compared with larvae from control fish (80%). eighty-nine-day-old larvae (mean weight 162.2 mg) from both groups were bath vaccina ... | 2005 | 15863015 |
| heme uptake genes in human and fish isolates of photobacterium damselae: existence of huta pseudogenes. | the marine bacterium photobacterium damselae includes strains classified into two distinct subspecies, namely subsp. damselae and subsp. piscicida, which have been reported to cause disease in a variety of marine animals and in humans. p. damselae strains utilize heme compounds as sole iron sources. in the present study, ten potential heme uptake and utilization genes are described in p. damselae subsp. damselae and subsp. piscicida. one gene cluster includes the genes coding for putative protei ... | 2005 | 15918073 |
| characteristics of endogenous flavin fluorescence of photobacterium leiognathi luciferase and vibrio fischeri nad(p)h:fmn-oxidoreductase. | the bioluminescent bacterial enzyme system nad(p)h:fmn-oxidoreductase-luciferase has been used as a test system for ecological monitoring. one of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. the use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from photobacterium leiognathi and nad(p)h:fmn-oxidoreductase from vibrio fischeri has been propo ... | 2005 | 15924327 |
| significant histamine formation in tuna (thunnus albacares) at 2 degrees c--effect of vacuum- and modified atmosphere-packaging on psychrotolerant bacteria. | occurrence and importance of psychrotolerant histamine producing bacteria in chilled fresh tuna were demonstrated in the present study. the objective was to evaluate microbial formation of histamine and biogenic amines in chilled fresh tuna from the indian ocean and stored either vacuum-packed (vp) or modified atmosphere-packed (map). firstly, biogenic amines and the dominating microbiota were determined in vp tuna involved in an outbreak of histamine fish poisoning in denmark. secondly, the mic ... | 2005 | 15925710 |
| a simple hydrophobicity-based approach to predict the toxicity of unknown organic micropollutant mixtures in marine water. | in this study, we propose a simple hydrophobicity-based approach to predict the toxicity of mixtures of marine organic micropollutants. fifty mixtures were chosen, comprising individual chemicals randomly chosen from three halogenated benzenes, three phenols, two petroleum hydrocarbons, three polychlorinated biphenyls (pcbs), four organochlorine pesticides (ocps) and two herbicides, with the intention of simulating the mixtures of organic micropollutants that may exist in some marine waters. the ... | 2005 | 15935177 |
| [conditions that influence bacterial luminescence in the presence of blood serum]. | conditions that influence the luminescence of natural and recombinant luminescent bacteria in the presence of blood serum were studied. in general, blood serum quenched the luminescence of the marine photobacterium phosphoreum and the recombinant escherichia coli strains harboring the luminescent system genes of photobacterium leiognathi, but enhanced the luminescence of the soil bacterium photorhabdus luminescens zm1 and the recombinant e. coli strain harboring the lux operon of p. luminescens ... | 2005 | 15938395 |
| interactive toxic effects of heavy metals and humic acids on vibrio fischeri. | the effect of humic acids (has) on the toxicity of copper, zinc, and lead was investigated using the photobacterium vibrio fischeri (microtox test) as a test organism. the effects of has on metal toxicity were evaluated as functions of time and concentration in pure compound solutions. the toxicities of copper and lead were generally comparable, while the toxicity of zinc was lower than those of the other two metals. the toxicity of copper decreased with the addition of has, while the toxicity o ... | 2006 | 15939470 |
| conjugal vectors for cloning, expression, and insertional mutagenesis in gram-negative bacteria. | 2005 | 15945369 | |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| update on bacterial vaccines: photobacterium damselae subsp. piscicida. | photobacterium damselae subsp. piscicida is the causative agent of pasteurellosis in wild and farmed marine fish worldwide. although serologically homogeneous, recent molecular advances have led to the discovery of distinct genetic clades, depending on geographical origin. further details of the strategies for host colonisation have arisen including information on the role of capsule, susceptibility to oxidative stress, confirmation of intracellular survival in host epithelial cells, and induced ... | 2005 | 15962471 |
| photobacterium damsela bacteremia in a child with sickle-cell disease. | photobacterium damsela was identified in a blood culture taken from a child with sickle-cell disease. this is the first report of this organism in humans in the caribbean. the microbiology of this organism and its identification are discussed. the clinical presentation in humans and the role of the immune status of the patient are reviewed. | 2005 | 15999016 |
| development of a simple and rapid fluorogenic procedure for identification of vibrionaceae family members. | we describe a simple colony overlay procedure for peptidases (copp) for the rapid fluorogenic detection and quantification of vibrionaceae from seawater, shellfish, sewage, and clinical samples. the assay detects phosphoglucose isomerase with a lysyl aminopeptidase activity that is produced by vibrionaceae family members. overnight cultures are overlaid for 10 min with membranes containing a synthetic substrate, and the membranes are examined for fluorescent foci under uv illumination. fluoresce ... | 2005 | 16000757 |
| photobacterium frigidiphilum sp. nov., a psychrophilic, lipolytic bacterium isolated from deep-sea sediments of edison seamount. | a gram-negative, motile, rod-shaped, psychrophilic and weakly halophilic bacterial strain, sl13(t), was isolated from deep-sea sediments (1450 m depth) of edison seamount in the western pacific ocean. optimal growth of sl13(t) requires the presence of 1.5% (w/v) nacl, a ph of 6.0 and a temperature of 14 degrees c. the whole-cell fatty acid profile of the isolate includes c16:1 and c16:0 as major fatty acids and contains c20:5omega3. this is consistent with corresponding data for photobacterium p ... | 2005 | 16014498 |
| synthesis, characterization, biocide and toxicological activities of di-n-butyl- and diphenyl-tin(iv)-salicyliden-beta-amino alcohol derivatives. | the one pot reaction of salicylaldehyde 1, beta-amino alcohols 2a-2c, and di-n-butyltin(iv) oxide 3a or diphenyltin(iv) oxide 3b produced five diorganotin(iv) compounds, 4a-4c, 5a, and 5c, in good yields. all compounds were characterized by ir, (1)h, (13)c, and (119)sn nmr spectroscopy, and elemental analysis; furthermore, compounds 4b, 4c, 5a, and 5c were characterized by x-ray diffraction analysis. after the structural characterization, all of the compounds were tested in vitro against bacillu ... | 2005 | 16022535 |
| coagulant toxicity and effectiveness in a slaughterhouse wastewater treatment plant. | attempts were made in this study to examine the toxicity of polymer/alum addition to the aeration tank effluent prior to sludge flotation as practiced in a slaughterhouse wastewater treatment plant. based on the microtox toxicity assay, alum at concentrations 100-200 mg/l was found to slightly increase the toxicity level of slaughterhouse wastewater effluent. however, at higher concentrations (300-1000 mg/l), significant residual chronic toxicity remained in all slaughterhouse wastewater effluen ... | 2006 | 16040124 |
| bioluminescence-mediated stimulation of photoreactivation in bacteria. | although biochemistry and genetics of light emission by cells have been investigated in detail, a biological role for bacterial luminescence has remained obscure for a long time. it was proposed recently that luminescence may stimulate dna repair, but the specific mechanism of this phenomenon was not investigated. moreover, experiments showing decreased survival of uv-irradiated lux mutants relative to luminescent cells were performed previously using only one bacterial species, vibrio harveyi. ... | 2005 | 16040205 |
| cloning and characterization of the rpoe gene encoding an rna polymerase sigmae factor from the deep-sea piezophilic shewanella violacea strain dss12. | the rpoe gene encoding an rna polymerase sigmae subunit was isolated from a gamma-phage library of the deep-sea piezophilic and psychrophilic bacterium shewanella violacea strain dss12. structual analysis showed that the gene organization of the fragment containing s. violacearpoe was the l-aspartate oxidase-coding gene, rpoe, rsea, rseb and rsec in that order, the same as in the case of photobacterium profundum ss9 and escherichia coli k-12. the cloned gene, 576 bp in length, was found to encod ... | 2005 | 16040350 |
| quorum sensing signal molecules (acylated homoserine lactones) in gram-negative fish pathogenic bacteria. | the aim of the present study was to investigate the production of quorum sensing signals (specifically acylated homoserine lactones, ahls) among a selection of strains of gram-negative fish bacterial pathogens. these signals are involved in the regulation of virulence factors in some human and plant-pathogenic bacteria. a total of 59 strains, representing 9 different fish pathogenic species, were tested against 2 ahl monitor bacteria (agrobacterium tumefaciens nt1 [pzlr4] and chromobacterium vio ... | 2005 | 16042042 |
| direct identification of photobacterium damselae subspecies piscicida by pcr-rflp analysis. | fish pasteurellosis is an infectious disease that affects several teleost species living in temperate marine waters. the pathogen responsible, photobacterium damselae subspecies piscicida, shows high genetic similarity with p. damselae subsp. damselae, making subspecies discrimination extremely laborious. here we report for the first time a pcr-rflp method for the identification of p. damselae subsp. piscicida without prior isolation in pure culture. genomic sequence information was obtained thr ... | 2005 | 16042043 |
| biochemical characterization of a cambialistic superoxide dismutase isozyme from diatom thallassiosira weissflogii: cloning, expression, and enzyme stability. | a cdna clone of 1081 bp encoding a second putative superoxide dismutase (sod) from diatom thallassiosira weissflogii was cloned by the polymerase chain reaction technique. the cdna encodes a protein of 286 amino acid residues. alignment of the truncated sod sequence containing 217 amino acid residues with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed greater homology to mn-sod. the residues required to coordin ... | 2005 | 16076113 |
| subtractive hybridization reveals a high genetic diversity in the fish pathogen photobacterium damselae subsp. piscicida: evidence of a sxt-like element. | photobacterium damselae subsp. piscicida is the causative agent of fish pasteurellosis, a severe disease affecting cultured marine fish worldwide. in this study, suppression subtractive hybridization was used to identify dna fragments present in the virulent strain pc554.2, but absent in the avirulent strain epoy 8803-ii. twenty-one genomic regions of this type (that included twenty-six distinct putative orfs) were analysed by dna sequencing. twenty orfs encoded proteins with homology to protein ... | 2005 | 16079344 |
| new cold-adapted lipase from photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases. | a photobacterium strain, m37, showing lipolytic activity, was previously isolated from an intertidal flat of the yellow sea in korea and identified as photobacterium lipolyticum sp. nov. in the present study, the corresponding gene was cloned using the shotgun method. the amino acid sequence deduced from the nucleotide sequence (1,023 bp) corresponded to a protein of 340 amino acid residues with a molecular weight of 38,026. no sequence similarity was found with any known bacterial lipases/ester ... | 2006 | 16088345 |
| pathogenicity of vibrios to rainbow trout (oncorhynchus mykiss, walbaum) and artemia nauplii. | the taxonomy of marine vibrios has changed rapidly over the last two decades, and a wealth of new species have been identified. many vibrio species are pathogenic to fish and crustaceans; however, little is known about the virulence of many of the novel species. the purpose of this study was to determine the ability of various recent isolates of vibrios to cause disease in rainbow trout (oncorhynchus mykiss, walbaum) and crustacea, i.e. artemia nauplii. of 56 isolates, representing 26 species of ... | 2005 | 16104871 |
| toxicity measurements in aqueous solution during ozonation of mono-chlorophenols. | the microtox toxicity and oxygen uptake rate (our) inhibition tests were conducted to monitor the variation of toxicity during ozonation of 2-chlorophenol (2-cp), 3-chlorophenol (3-cp) and 4-chlorophenol (4-cp) under neutral conditions. the results revealed that the oxidized 2-cp solution exhibited new toxicity to pure bacteria and mixed microorganisms in the early stage of ozonation. the largest inhibition of our appeared at one mol of applied ozone dosage per mol of initial 2-cp, and the perce ... | 2006 | 16122866 |
| phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. | we analyzed the usefulness of rpoa, reca, and pyrh gene sequences for the identification of vibrios. we sequenced fragments of these loci from a collection of 208 representative strains, including 192 well-documented vibrionaceae strains and 16 presumptive vibrio isolates associated with coral bleaching. in order to determine the intraspecies variation among the three loci, we included several representative strains per species. the phylogenetic trees constructed with the different genetic loci ... | 2005 | 16151093 |
| phylogenetic resolution and habitat specificity of members of the photobacterium phosphoreum species group. | substantial ambiguity exists regarding the phylogenetic status of facultatively psychrophilic luminous bacteria identified as photobacterium phosphoreum, a species thought to be widely distributed in the world's oceans and believed to be the specific bioluminescent light-organ symbiont of several deep-sea fishes. members of the p. phosphoreum species group include luminous and non-luminous strains identified phenotypically from a variety of different habitats as well as phylogenetically defined ... | 2005 | 16156737 |
| detection of quinolone-resistance genes in photobacterium damselae subsp. piscicida strains by targeting-induced local lesions in genomes. | quinolone-resistant strains of the fish-pathogenic bacterium, photobacterium damselae subsp. piscicida are distributed widely in cultured yellowtail, seriola quinqueradiata (temminck & schlegel), in japan. the quinolone resistance-determining region (qrdr) was amplified with degenerate primers, followed by cassette ligation-mediated pcr. open reading frames encoding proteins of 875 and 755 amino acid residues were detected in the gyra and parc genes, respectively. resistant strains of p. damsela ... | 2005 | 16159364 |
| laterally transferred elements and high pressure adaptation in photobacterium profundum strains. | oceans cover approximately 70% of the earth's surface with an average depth of 3800 m and a pressure of 38 mpa, thus a large part of the biosphere is occupied by high pressure environments. piezophilic (pressure-loving) organisms are adapted to deep-sea life and grow optimally at pressures higher than 0.1 mpa. to better understand high pressure adaptation from a genomic point of view three different photobacterium profundum strains were compared. using the sequenced piezophile p. profundum strai ... | 2005 | 16162277 |
| purification and characterization of recombinant caulobacter crescentus cu,zn superoxide dismutase. | recombinant cu,zn superoxide dismutase from caulobacter crescentus has been expressed in escherichia coli and characterized. the corresponding recombinant protein has a molecular weight typical of a homodimeric cu,znsods and an activity comparable to that of other prokaryotic enzymes. the copper active site is characterized by a peculiar axial geometry as evidenced by its electron paramagnetic resonance spectrum, moreover, the copper atom displays a low accessibility toward external chelating ag ... | 2006 | 16213200 |
| effect of salinity on the immune response of tiger shrimp penaeus monodon and its susceptibility to photobacterium damselae subsp. damselae. | addition of nacl at 2.5% to tryptic soy broth (tsb) significantly increased the growth of photobacterium damselae subsp. damselae. tiger shrimp penaeus monodon held in 25 per thousand seawater were injected with p. damsela subsp. damselae grown in tsb containing nacl at 0.5%, 1.5%, 2.5% and 3.5% at a dose of 8.48 x 10(4)colony-forming units (cfu)shrimp(-1). over 24-96 h, the cumulative mortality was significantly higher for the shrimp challenged with p. damselae subsp. damselae grown in 2.5% nac ... | 2006 | 16213751 |
| vibrionaceae as a possible source of qnr-like quinolone resistance determinants. | to gain insight into the functionality of qnr-like genes of several bacterial species of vibrionaceae that may encode quinolone resistance determinants. | 2005 | 16227349 |
| detoxication of solutions of organic oxidants by humic substances: bioluminescence monitoring. | 2005 | 16229147 | |
| statistical optimization of bioluminescence of photobacterium phosphoreum kctc2852. | in this study, media optimization by statistically designed experiments stimulated an increase in the intensity and stability of bioluminescence of photobacterium phosphoreum kctc 2852 during continuous cultivation. the plackett-burman design method selected two components (nacl, yeast extract) among nine components of the seawater complete (sc) medium. the response-surface design method calculated the optimum concentration of nacl (31.1 g/l) and yeast extract (5.1 g/l). in the optimized medium, ... | 2001 | 16233061 |
| [bacterial flora composition and its dynamics in tidal-flat sinonovacula constricta aquaculture area]. | in this study, the bacteria from the mud in tidal-flat sinonovacula constricta aquaculture area were isolated each month from march to december, 2002, and the temporal and spatial distribution of heterotrophic bacteria, ammonifying bacteria, denitrifying bacteria, and sulphate reducing bacteria were analyzed. the results showed that all the 515 isolated bacteria mainly belonged to 1 family and 13 genera. the bacterial flora in different layers of the mud was almost consistent, while the composit ... | 2005 | 16252888 |
| aip56, a novel plasmid-encoded virulence factor of photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils. | a strategy used by extracellular pathogens to evade phagocytosis is the utilization of exotoxins that kill host phagocytes. we have recently shown that one major pathogenicity strategy of photobacterium damselae subsp. piscicida (phdp), the agent of the widespread fish pasteurellosis, is the induction of extensive apoptosis of sea bass macrophages and neutrophils that results in lysis of these phagocytes by post-apoptotic secondary necrosis. here we show that this unique process is mediated by a ... | 2005 | 16262788 |
| complement consumption by photobacterium damselae subsp. piscicida in seabream, red porgy and seabass normal and immune serum. effect of the capsule on the bactericidal effect. | a virulent strain of photobacterium damselae subsp. piscicida (pdp) was grown without (c form) or with (c+ form) glucose supplementation, the latter to enhance capsule formation. both forms were resistant to killing by normal serum of seabream, red porgy and seabass. however, the c form was killed by immune serum of all three fish species while the c+ form was killed only by seabream and red porgy sera and to a lesser extent than the c form. both c and c+ forms consumed complement in normal seru ... | 2006 | 16263309 |
| photobacterium aplysiae sp. nov., a lipolytic marine bacterium isolated from eggs of the sea hare aplysia kurodai. | a bacterium, named gmd509t, showing lipolytic activity was isolated from the eggs of the sea hare aplysia kurodai collected at mogiyeo (depth, 12 m), an uninhabited small island in the south sea of korea. the strain is gram-negative, motile, facultatively anaerobic, mesophilic and weakly halophilic. optimal growth of strain gmd509t occurs in the presence of 3.0 % (w/v) nacl and at ph 8 and 25 degrees c. the whole-cell fatty acid profile of the isolate includes c16 : 1, c16 : 0 and c18 : 1 as maj ... | 2005 | 16280485 |
| random isotopolog libraries for protein perturbation studies. 13c nmr studies on lumazine protein of photobacterium leiognathi. | [graph: see text] lumazine proteins of luminescent bacteria are paralogs of riboflavin synthase which are devoid of catalytic activity but bind the riboflavin synthase substrate, 6,7-dimethyl-8-ribityllumazine, with high affinity and are believed to serve as optical transponders for bioluminescence emission. lumazine protein of photobacterium leiognathi was expressed in a recombinant escherichia coli host and was reconstituted with mixtures (random libraries) of 13c-labeled isotopologs of 6,7-di ... | 2005 | 16292826 |
| suitability of bifidobacteria and thymol as biopreservatives in extending the shelf life of fresh packed plaice fillets. | the present study was aimed to evaluate the microbiological and sensory characteristics of biopreserved packed fresh plaice (pleuronectes platessa), during storage. | 2005 | 16313401 |
| quantitative analysis of bacterial communities from mediterranean sapropels based on cultivation-dependent methods. | microbial communities of ancient mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (mpn) approaches. mineral media containing different carbon sources in sub-millimolar concentrations were used. mpn numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. highest mpn counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-cha ... | 2004 | 16329860 |
| seasonal and geographic distribution of luminous bacteria in the eastern mediterranean sea and the gulf of elat. | luminous bacteria in the mediterranean sea and the gulf of aqaba-elat have different distribution patterns. in the mediterranean sea, beneckea harveyi is present all year round, with different subtypes alternating in summer and winter; photobacterium fischeri was only present during the winter. in the gulf of elat, p. leiognathi is present throughout the water column in similar densities during the entire year. this constancy in distribution is presumably due to the near-constancy in water tempe ... | 1979 | 16345404 |
| luminous enteric bacteria of marine fishes: a study of their distribution, densities, and dispersion. | three taxa of luminous bacteria (photobacterium fischeri, p. phosphoreum, and beneckea spp.) were found in the enteric microbial populations of 22 species of surface- and midwater-dwelling fishes. these bacteria often occurred in concentrations ranging between 10 and 10 colony-forming units per ml of enteric contents. by using a genetically marked strain, it was determined that luminous cells entering the fish during ingestion of seawater or contaminated particles traversed the alimentary tract ... | 1979 | 16345429 |
| physiological characteristics underlying the distribution patterns of luminous bacteria in the mediterranean sea and the gulf of elat. | physiological characteristics of luminous bacteria isolated from the mediterranean and gulf of elat were compared to determine their relationship to the specific seasonal and geographic distribution patterns of these bacteria. the effects of temperature on growth rate and yield, relative sensitivity to photooxidation, resistance to high salt concentration (8%), and ability to grow in nutrient-poor conditions appear to control these patterns. the winter appearance of photobacterium fischeri and t ... | 1979 | 16345442 |