Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [inhibitory analysis of the luminescent electron transport chain of photobacterium fischeri]. | the quenching of luminescence of bacterial luciferase from photobacterium fischeri by non-specific electron acceptors and inhibitors of dehydrogenases was studied. the inhibition of the luminescent reaction obeys the non-competitive mechanism with nadh, fmn and aliphatic aldehyde. the inhibitors compete with cytochrome c for nadh -- cytochrome c oxido-reductase. it is concluded that lumiredoxin, a fes-containing protein, is the most sensitive component of the luminescent electron transport chain ... | 1981 | 7248374 |
| [inhibition of bacterial luminescence by cytochrome p-450 substrates]. | the mechanisms of luminescence quenching by various drugs, e.g. dimethylaniline, ethylmorphine, hexobarbital and aminopyrine, which are effective inhibitors of luminescence both in intact cells and in bacterial luciferase, were studied. it was shown that the inhibition of luminescence occurs due to competition of the bacterial luminescence system substrate--aliphatic aldehyde in cytochrome p-450. the functional similarity of the bacterial luminescence system to the microsomal hydroxylation syste ... | 1981 | 7248381 |
| use of the luminescent bacterial system for the rapid assessment of aquatic toxicity. | a simple and reliable method for monitoring the toxicity of aquatic samples has been developed. the assay is based on changes in the light output of luminescent bacteria, as measured by a temperature controlled photometric device. the new assay method described here correlates well with other bioassays yet requires less than thirty minutes to obtain a complete reportable assay. the assay system is an instrumental approach in which the bioassay organisms are handled like a chemical reagent. data ... | 1981 | 7251338 |
| dna-damaging agents and dna-synthesis inhibitors induce luminescence in dark variants of luminous bacteria. | the dna-damaging agents mitomycin c and uv irradiation, as well as the dna-synthesis inhibitors nalidixic acid, novobiocin and coumermycin, induce the de novo synthesis of luciferase and in vivo luminescence in dark variant cells of the luminous bacteria photobacterium leiognathi. mitomycin c and nalidixic acid also cause the induction of luminescence in wild-type cells in the absence of its natural inducer. in spite of the high level of in vivo luminescence of the treated dark-variant cells, no ... | 1981 | 7290100 |
| fatty acid reductase in bioluminescent bacteria. resolution from aldehyde reductases and characterization of the aldehyde product. | fatty acid reductase from the bioluminescent bacterium photobacterium phosphoreum, has been partially purified free of aldehyde reductase activity and with a low endogenous fatty acid content permitting the characterization of the aldehyde product of the reaction. two aldehyde reductases, both dependent on nadh, were separated by anion-exchange chromatography from the fatty acid reductase activity. the partially purified fatty acid reductase catalyzed the synthesis exclusively of long chain alde ... | 1981 | 7296339 |
| a membrane-covered photobacterium probe for oxygen measurements in the nanomolar range. | 1981 | 7304978 | |
| [pyruvic acid formation by the luminescent bacterium photobacterium mandapamensis]. | 1981 | 7321908 | |
| chemistry and biochemistry of superoxide dismutases. | the univalent reduction of oxygen to the superoxide radical is a commonplace event in biological systems, and the superoxide dismutases, which catalytically scavenge this radical, are the primary defence against its potential cytotoxicity. the superoxide radical and hydrogen peroxide, can interact to generate the hydroxyl radical. superoxide dismutases are metalloenzymes that can prevent the generation of hydroxyl radical by keeping the level of superoxide radical vanishingly low. superoxide dis ... | 1981 | 7343318 |
| adjuvant effect of photobacterium phosphoreum pj-1 on humoral immune response of ddy mice to sheep erythrocytes. | 1981 | 7349282 | |
| [effect of amino acids on the luminescent system induction in photobacterium belozerskii]. | variations in the intensity of luminescence of photobacterium belozerskii grown on different media were studied. in the course of growth the luminescence intensity changed by 2 to 4 orders of magnitude, depending on the nutrient medium used. exogenous myristic aldehyde added to the bacterial suspension at the time of luminescence measurement decreased the intensity to a degree, which was essentially independent from the initial level. the onset of an increase in the luminescence intensity depend ... | 1980 | 7384006 |
| [electron transport systems of photobacterium fischeri]. | the composition of cytochromes was studied in photobacterium fischeri 6 at different growth phases and under various conditions of cultivation. the electron transport chains of the bacterium are characterized by the presence of cytochromes b and c types. the terminal oxidases are cytochromes o, a2+a1 and p-450. the hemoprotein p-450 functions as a mixed function oxidase. the qualitative composition of cytochromes does not depend on the growth phase of the bacterium but does on the conditions of ... | 1980 | 7402117 |
| lumazine protein from the bioluminescent bacterium photobacterium phosphoreum. purification and characterization. | lumazine protein, a novel protein containing 6,7-dimethyl-8-ribityllumazine as a bound prosthetic group, is one of the several major proteins produced by the bioluminescent bacteria, photobacterium phosphoreum. purification to complete homogeneity from cell extracts is achieved in six steps. lumazine protein is a near spherical, monomeric protein of average molecular weight 20,000; in amino acid composition it is acidic with two isoelectric isomers, pi 4.9 and 5.0, and is hydrophilic (974 cal/re ... | 1980 | 7410396 |
| [cytochromes of the luminescent bacterium, photobacterium fischeri, their solubilization and relationship to luminescence]. | the hemoprotein composition of the luminescent bacterium photobacterium fischeri was studied, in particular, the distribution of cytochromes among the bacterial fractions, viz. cell-free extract, supernatant, "particles", protein preparation. the hemochromogenic analysis has shown that the principal hemoproteins of photobacterium fischeri are cytochromes, with hemes of the b and c type. the activity of luciferase is distributed with hemoproteins. the purified preparation of luciferase contains c ... | 1980 | 7412614 |
| [luciferase synthesis regulation in photobacterium mandapamensis]. | the synthesis of luciferase and the dynamics of luminescence were studied in the course of batch cultivation of the strain 54-k obtained from the wild strain of photobacterium mandapamensis after numerous passages. luciferase synthesis bvy the strain was not sensitive to the inhibitor contained in the growth medium and did not require the accumulation of an "audoinductor". since the intensity of luminescence and the content of luciferase per cell did change, the bacterium seemed to possess an ad ... | 1980 | 7412616 |
| lumazine protein from the bioluminescent bacterium photobacterium phosphoreum. a fluorescence study of the protein-ligand equilibrium. | the changes of fluorescence spectral distribution, polarization, and lifetime of the lumazine protein from photobacterium phosphoreum can be interpreted in terms of an equilibrium between the protein and its dissociated prosthetic group 6,7-dimethyl-8-(1'-d-ribityl)lumazine. the equilibrium is rapidly attained, 1:1, and kd is 5 x 10(-8) m (4 degrees c, ph 7, 67 mm phosphate). a change in solution conditions like an increase in temperature or dilution or a decrease in ph or ionic strength favors ... | 1980 | 7417412 |
| bioluminescence and cell growth of photobacterium phosphoreum. | the bioluminescence activity of photobacterium phosphoreum was compared at different times after cell division by the methods of density gradient centrifugation and synchronous culture. the bioluminescence intensity per cell mass increased linearly at a rate of 1.5 times per doubling time. the luciferase system in the cell is continuously activated during growth, independent of cell division. | 1980 | 7419524 |
| protein-ligand interactions in lumazine protein and in desulfovibrio flavodoxins from resonance coherent anti-stokes raman spectra. | the resonance coherent anti-stokes raman technique was used to obtain vibrational spectra of flavin in flavodoxins from desulfovibrio gigas and desulfovibrio vulgaris and of the simpler 6,7-dimethyl-8-ribityllumazine chromophore in the blue fluorescence lumazine protein from the bioluminescent bacterium photobacterium phosphoreum. in the region examined, 1100-1700 cm-1, the raman spectrum of the lumazine is less crowded than that of the flavin and this facilitates assignment of observed frequenc ... | 1980 | 7426621 |
| co-induction of fatty acid reductase and luciferase during development of bacterial bioluminescence. | the luminescent bacterium photobacterium phosphoreum has been shown to possess a fatty acid reductase based on the stimulation of the aldehyde-dependent luminescent reaction on incubation of the enzyme with atp, nadph, and tetradecanoic acid (riendeau, d., and meighen, e. (1979) j. biol. chem. 254, 7488-7490). a direct, luciferase-independent assay for the fatty acid reductase has now been developed using [3h]tetradecanoic acid as substrate and thin layer chromatography to separate and identify ... | 1980 | 7440587 |
| photokinetic microanalysis of nadp+, using bacterial luciferase. | bioluminescence photokinetic assay of nadp+ is described, using the glucose-6-phosphate dehydrogenase reaction for conversion to its reduced form and subsequent measurement of this with luciferase extracts of vibria fisherii. the analyses were applied to the determination of the activity of minute amounts of glutathione reductase using nadp+ as measurable product and for nucleotide assay in cell samples of 0.5--10 microgram dry weight. the sensitivity was sufficient for determining 0.5 picomoles ... | 1980 | 7442656 |
| formation of hybrid luciferases from subunits of different species of photobacterium. | enzyme divergence within three species of the genus photobacterium (p. fischeri, p. leiognathi, and p. phosphoreum) was studied by comparing the catalytic characteristics and quaternary interactions of bacterial luciferases isolated from each species. each luciferase was composed of two subunits of different molecular weights as determined by sodium dodecyl sulfate--polyacrylamide gel electrophoresis. subunits were isolated in quantity by deae-sephadex gel filtration in 7 m urea. isolated subuni ... | 1980 | 7459320 |
| the bacterial 'enigma': cracking the code of cell-cell communication. | in recent years it has become clear that the production of n-acyl homoserine lactones (n-ahls) is widespread in gram-negative bacteria. these molecules act as diffusible chemical communication signals (bacterial pheromones) which regulate diverse physiological processes including bioluminescence, antibiotic production, plasmid conjugal transfer and synthesis of exoenzyme virulence factors in plant and animal pathogens. the paradigm for n-ahl production is in the bioluminescence (lux) phenotype o ... | 1995 | 7476157 |
| biochemistry of bacterial bioluminescence. | 1995 | 7480148 | |
| modelling of microbial activity and prediction of shelf life for packed fresh fish. | prediction of shelf life based on growth of specific spoilage organisms (sso) in model substrates was studied. the effect of co2 on the growth kinetics for photobacterium phosphoreum and shewanella putrefaciens was quantified and modelled. results showed that microbial spoilage of packed cod stored with various concentrations of co2 was accurately predicted from the effect of co2 on p. phosphoreum grown in model substrates. the short shelf life extensions previously reported for packed cod there ... | 1995 | 7488526 |
| qualitative and quantitative characterization of spoilage bacteria from packed fish. | the large cells recently suggested to be responsible for spoilage of packed cod, have been identified as photobacterium phosphoreum. the spoilage activity of these cells, of shewanella putrefaciens and of other microorganisms isolated form spoiled packed cod has been studied. both qualitative and quantitative tests were used for characterization of the microbial spoilage activity. the importance of the different groups of microorganisms was evaluated by comparison of microbial spoilage activity ... | 1995 | 7488527 |
| characterisation of the yeni/yenr locus from yersinia enterocolitica mediating the synthesis of two n-acylhomoserine lactone signal molecules. | yersinia enterocolitica produces compounds capable of transcriptionally activating the photobacterium fischeri bioluminescence (lux) operon. using high-performance liquid chromatography, high resolution tandem mass spectrometry in conjunction with chemical synthesis, two signal molecules were identified and shown to be n-hexanoyl-l-homoserine lactone (hhl) and n-(3-oxohexanoyl)-l-homoserine lactone (ohhl). a gene (yeni) was isolated from y. enterocolitica and demonstrated to direct the synthesis ... | 1995 | 7494483 |
| the lumq gene is linked to the lump gene and the lux operon in photobacterium leiognathi. | the nucleotide sequence of the designated lumq gene (embl accession no. u35231) from photobacterium leiognathi pl741 has been determined, and the encoded amino acid sequence is deduced. the lumq protein has a calculated m(r) of 28,416 and comprises 248 amino acid residues. the lumq gene is identified as the envy-like gene by significant similarity of the encoded protein with the envy and adiy proteins of e. coli; there the envy gene encodes the porin thermoregulatory protein envy, and the adiy g ... | 1995 | 7503752 |
| phylogenetic analysis and assessment of the genera vibrio, photobacterium, aeromonas, and plesiomonas deduced from small-subunit rrna sequences. | we sequenced nearly complete small-subunit rrnas of 54 reference strains belonging to the genera vibrio, photobacterium, aeromonas, and plesiomonas. we then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony ... | 1994 | 7520733 |
| phototoxicology. 2. near-ultraviolet light enhancement of microtox assays of trinitrotoluene and aminodinitrotoluenes. | coexposure of 2,4,6-trinitrotoluene (tnt), 2-amino-4,6-dinitrotoluene (2a), or 4-amino-2,6-dinitrotoluene (4a) to near-ultraviolet (nuv) light (lambda max-354 nm) significantly enhanced their toxicity toward photobacterium phosphoreum (microtox bioassay) during 30 min but not 15 min. based on the slopes of the dose-response lines, the nuv coexposure and dark toxic mechanisms of action for tnt, 2a, and 4a appeared to be similar. nuv coexposure of binary mixtures significantly enhanced (supraaddit ... | 1994 | 7525202 |
| the environmental risks of industrial waste disposal: an experimental approach including acute and chronic toxicity studies. | the toxicity of 15 leachates of various solid industrial wastes accepted in an engineered landfill has been studied. a cost-effective battery of tests allowing evaluation of acute and chronic toxicity, as well as genotoxicity, and investigations on different trophic levels in the aquatic environment has been used. acute toxicity was tested on bacteria (microtox assay with photobacterium phosphoreum) and microcrustaceans (daphnia magna immobilization assay). a growth inhibition test of microalgae ... | 1994 | 7525226 |
| small-subunit rrna sequences and whole dna relatedness concur for the reassignment of pasteurella piscicida (snieszko et al.) janssen and surgalla to the genus photobacterium as photobacterium damsela subsp. piscicida comb. nov. | the taxonomic status of pasteurella piscicida (strain ncimb 2058t [t = type strain] and a strain isolated from the environment) was investigated by performing phylogenetic analyses of small-subunit rrna sequences, dna-dna hybridization analyses, and biochemical characterization analyses. the results of the phylogenetic analyses and the levels of dna-dna complementarity demonstrated conclusively that pasteurella piscicida is extremely closely related to photobacterium damsela atcc 33539t. since t ... | 1995 | 7531996 |
| mechanism-based comparisons of acute toxicities elicited by industrial organic chemicals in procaryotic and eucaryotic systems. | comparisons of toxicities elicited by nonpolar and polar narcotics, weak acid uncouplers of oxidative phosphorylation, and bioreactive chemicals between the eucaryotic systems pimephales promelas and tetrahymena pyriformis and the procaryotic systems escherichia coli and photobacterium phosphoreum were performed. each chemical had been a priori assigned a mechanism/mode of action based on the results from previous studies with eucaryotic systems. hydrophobicity-dependent qsars for nonpolar narco ... | 1994 | 7533711 |
| ketone ec50 values in the microtox test. | the microtox ec50 values for the following ketones are reported in the following homologous series: straight chain methyl ketones (acetone, 2-butanone, 2-pentanone, 2-hepatonone, 2-octanone, 2-decanone, and 2-tridecanone); methyl ketones substituted at one alpha carbon (3-methyl-2-butanone; 3,3-dimethyl-2-butanone); methyl substituted at two alpha carbons (2,4-dimethyl-3-pentanone; 2,2,4,4-tetramethyl-3-pentanone); phenyl groups replacing methyl in acetone (acetophenone; benzophenone); methyl gr ... | 1995 | 7539364 |
| review of whole-organism bioassays: soil, freshwater sediment, and freshwater assessment in canada. | whole organism bioassays for the assessment of soil, freshwater sediment, and freshwater quality were evaluated for their application in the assessment and remediation of contaminated sites in canada under the national contaminated sites remediation program. using 3 essential and 12 desirable methodological criteria, bioassays were categorized as currently usable, prototype, or under development. based on further considerations related to bioassay application, a battery of usable screening and d ... | 1995 | 7541337 |
| acute aquatic toxicity of protolyzing substances studied as the microtox effect. | in tests of acute aquatic toxicity, the effects of protolyzing substances varies with ph. the microtox toxicity of six chlorophenols was studied over a ph interval and the relation of effects to dissociation was examined. the pka values of the chlorophenols under the optimal test conditions are presented. the results of the microtox toxicity tests supported a simple model of two species, phenol and phenate, with different specific toxicity. in five of the six chlorophenols only the effects of th ... | 1995 | 7541342 |
| relationship of aquatic natural organic material characteristics to the toxicity of selected insecticides. | toxicities of the commercial insecticide formulations of azinphos methyl, chlorpyrifos, fenvalerate, and methyl parathion were evaluated in streamwater samples containing natural organic material (nom) using a modification of a bacterial bioluminescence assay. toxicity reduction of azinphos methyl was significantly (p < 0.05) correlated with water sample nonvolatile total solids (nvts) concentration. toxicity reductions of fenvalerate and of methyl parathion were significantly correlated to e4/e ... | 1995 | 7544270 |
| the yellow bioluminescence bacterium, vibrio fischeri y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence fmn protein. | the yellow bioluminescence y1 strain of vibrio fischeri can produce a 22 kda protein with either fmn or riboflavin as a bound fluorophore. both forms are active for shifting the bioluminescence spectral maximum. the fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. the bioluminescence activity of the riboflavin y1 protein contrasts with the inactivity of the related photobacterium type. | 1995 | 7598706 |
| crystal structure determination of a flavoprotein fp390 from a luminescent bacterium, photobacterium phosphoreum. | the three-dimensional structure of a flavoprotein, fp390, purified from a luminescent bacterium, photobacterium phosphoreum, has been determined at 3 a resolution by x-ray crystallography. crystallographic refinements of the structural model have led to an r-factor of 0.24 for the intensity data between 6 to 3 a resolution collected with synchrotron radiation. it was found that a homodimer of the fp390 molecules related by a non-crystallographic 2-fold axis is comprised in the asymmetric unit. t ... | 1995 | 7629024 |
| assessment of rapid bioassays for detecting cyanobacterial toxicity. | simple and easy-to-use bioassays with artemia salina (brine shrimp) larvae, luminescent bacteria and pseudomonas putida were evaluated for the detection of toxicity due to cyanobacterial hepato- and neurotoxins. the hepatotoxins and a neurotoxin, anatoxin-a, were extracted from laboratory-grown cultures and natural bloom samples by the solid phase fractionation method and dissolved in diluent for different bioassays. the toxin concentration of cyanobacterial extracts was determined with hplc. th ... | 1995 | 7639991 |
| technical note: bioluminescent bacterial test for acute toxicity: the effect of ph and buffer solutions. | 1995 | 7640442 | |
| isolation of cdnas encoding gtp cyclohydrolase ii from arabidopsis thaliana. | a gtp cyclohydrolase ii-encoding gene from arabidopsis thaliana was isolated through functional complementation of a mutant of escherichia coli, bsv18, deficient in this protein. the derived amino-acid sequence constitutes a polypeptide of 27 kda and shows 37-58% identity with previously published sequences of escherichia coli, bacillus subtilis, photobacterium leiognathi and p. phosphoreum. | 1995 | 7642114 |
| quantitative structure-activity relationships as a tool to assess the comparative toxicity of organic chemicals. | quantitative structure-activity relationships of toxicity are discussed as a means of assessing the value of the microtox test which uses the light-emitting bacterium vibrio fisheri (photobacterium phosphoreum) as a replacement for toxicity testing in higher species. the microtox test is found to be a good surrogate for testing in fish, for compounds acting by the narcosis mechanism. however, for reactive chemicals the microtox test significantly underestimates the potential hazard. it should no ... | 1995 | 7670864 |
| formation of active bacterial luciferase between interspecific subunits in vivo. | interspecific complementation between luxas and luxbs from vibrio harveyi, vibrio fischeri, photobacterium leiognathi and xenorhabdus luminescens was examined in vivo. the individual genes from these species were cloned on different compatible plasmids or amplified by pcr and brought together to yield cis combinations without extraneous dna. the beta subunits from v. harveyi and x. luminescens form active enzyme only with alpha subunits from one of these species. all other combinations yield act ... | 1995 | 7676858 |
| marine biology. light genes will out. | 1993 | 7683389 | |
| bioluminescent symbionts of flashlight fishes and deep-sea anglerfishes form unique lineages related to the genus vibrio. | bioluminescent symbioses range from facultative associations to highly adapted, apparently obligate ones. the family anomalopidae (flashlight fishes) encompasses five genera of tropical reef fishes that have large suborbital light organs. the suborder ceratioidei (deep-sea anglerfishes) contains 11 families. in nine of these, females have a bioluminescent lure that contains bacterial symbionts. in all other fish light-organ symbioses (occurring in 10 families in 5 orders), the symbionts belong t ... | 1993 | 7683390 |
| toxicity of sediments and sediment pore waters from the grand calumet river-indiana harbor, indiana area of concern. | the assessment of contaminated sediments is a difficult task due to the complex nature of the sediment matrix and the potential for exposure of aquatic organisms to in-place contaminants via several routes. differential species sensitivity also precludes the completion of a meaningful environmental assessment with only one species. therefore, a battery of assays approach with the microtox assay, 48 hr daphnia magna and ceriodaphnia dubia tests and a 10-day chironomous tentans test was used to ev ... | 1993 | 7691537 |
| common structural features of the luxf protein and the subunits of bacterial luciferase: evidence for a (beta alpha)8 fold in luciferase. | the amino acid sequence identity and potential structural similarity between the subunits of bacterial luciferase and the recently determined structure of the luxf molecule are examined. the unique beta/alpha barrel fold found in luxf appears to be conserved in part in the luciferase subunits. from secondary structural predictions of both luciferase subunits, and from structural comparisons between the protein product of the luxf gene, nfp, and glycolate oxidase, we propose that it is feasible f ... | 1994 | 7703838 |
| interactions between aldehyde derivatives and the aldehyde binding site of bacterial luciferase. | the interaction of triazine aldehydes with the aldehyde binding site of bacterial luciferases was investigated using a series of triazine aldehydes with different aldehyde chain length, and substituents on the s-triazine ring. substrate activity was determined using luciferase from photobacterium fischeri and vibrio harveyi in a dithionite-based luciferases assay. the chain length optimum was determined for two triazine aldehyde classes to be c-10 and c-11, respectively. only the substrate activ ... | 1995 | 7762412 |
| nucleotide sequence and functional analysis of regulatory region of the lump and the lux operon from photobacterium leiognathi. | the lump gene is linked to the lux operon, but runs in the opposite direction in photobacterium leiognathi pl741. the gene order of the lump and the lux operon is < -lump-r & r-luxc-luxd-luxa-luxb-luxn-luxe- > (r & r: regulatory region). the nucleotide sequence of the regulatory region (827-bp) between the lump and the lux operon was determined. sequence analysis illustrates that the regulatory region includes two divergent promoter systems, pr-promoter system for the lux operon (r-operon) and p ... | 1995 | 7763266 |
| immobilized cells used for detection and analysis. | many kinds of biosensor have now been developed and utilized for various types of analysis including clinical, medical and environmental monitoring, industrial process control, as well as many other applications. the microbial biosensor has advantages, such as longer lifetime and lower cost, over other types of biosensor. recently, photobacteria and recombinant bacteria have been employed in biosensors both for determining biochemical oxygen demand and for detecting heavy metals and other toxic ... | 1994 | 7764644 |
| structural refinement of the non-fluorescent flavoprotein from photobacterium leiognathi at 1.60 a resolution. | the crystallographically-determined structure of the non-fluorescent flavoprotein (nfp) from photobacterium leiognathi, a homolog of the bacterial luciferase subunits, has been refined to a conventional r-factor [formula: see text] of 0.175 using synchrotron data between 10.0 and 1.60 a resolution. the molecular structure is a homodimer of beta/alpha domains, the monomer having structural similarities to (beta alpha)8 barrel proteins. however, one beta-strand and three alpha-helices of a typical ... | 1995 | 7776372 |
| relative sensitivity of some selected aquatic organisms to phenol. | 1995 | 7780215 | |
| genetic characterization of omph mutants in the deep-sea bacterium photobacterium sp. strain ss9. | omph is an outer membrane protein produced by the deep-sea bacterium photobacterium species strain ss9 in response to elevated hydrostatic pressure. in order to facilitate studies of the function of this protein, a series of omph+ and omph- strains were obtained from ss9 by tn5 gene replacement mutagenesis. a previously isolated omph::lacz strain and a derivative of this strain harboring a plasmid expressing the wild-type omph gene were also utilized. the acridine mutagen icr 191 preferentially ... | 1994 | 7857197 |
| omph gene expression is regulated by multiple environmental cues in addition to high pressure in the deep-sea bacterium photobacterium species strain ss9. | photobacterium species strain ss9 is a moderately barophilic (pressure-loving) deep-sea bacterial species which induces the expression of the omph gene in response to elevated pressure. here we demonstrate that at 1 atm (1 atm = 1.01325 x 10(5) pa), omph expression increases with cell density in 2216 marine medium batch culture and is subject to catabolite repression and the omph synthesis is inducible by energy (carbon) starvation. regulatory mutants which are impaired in omph gene expression a ... | 1995 | 7860581 |
| penaeid prawns and associated luminous bacteria. | luminous bacteria associated with the exoskeleton, gill and gut of penaeid prawns, penaeus indicus h. milne edwards and penaeus monodon fabricius in mangalore waters were isolated, identified and quantified. two species of bacteria, vibrio harveyi and vibrio fischeri were recorded, the former was dominant in the exoskeleton and gill of both the penaeids. gut of prawns supported exclusive and dense populations of v. harveyi suggesting that the species is well adapted to proliferate in this microe ... | 1994 | 7866726 |
| a 26-kda outer membrane protein, ompk, common to vibrio species is the receptor for a broad-host-range vibriophage, kvp40. | kvp40 is a broad-host-range vibriophage forming plaques on strains of at least eight vibrio and one photobacterium species. a spontaneous kvp40-resistant mutant, r4000, derived from vibrio parahaemolyticus 1010 lacked a 26-kda outer membrane protein designated ompk. kvp40 was inactivated by outer membrane and ompk prepared from 1010, but not by outer membrane from r4000. these results strongly suggest that ompk is the receptor for kvp40. immunoblotting analyses using an anti-ompk rabbit serum re ... | 1995 | 7867914 |
| properties of recombinant fluorescent proteins from photobacterium leiognathi and their interaction with luciferase intermediates. | ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (embl x56534) of photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and sds-page. scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30 degrees c the kds (microm) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound fm ... | 1995 | 7880825 |
| ecotox-evaluation strategy for soil bioremediation exemplified for a pah-contaminated site. | during a bioremediation of a pah-contaminated site chemical and biological analyses were carried out. the biological investigations included ecotoxicological analyses in the aqueous extract, (pseudomonas putida, photobacterium phosphoreum, daphnids, algae, fish) and analyses in the soil with introduced organisms (plants, earthworms) and natural soil organisms (nematodes, microorganisms). in all test systems a correspondence between decreasing toxicity and degradation of the easily biodegradable ... | 1994 | 7922149 |
| uv-a coexposure enhances the toxicity of aromatic hydrocarbons, munitions, and metals to photobacterium phosphoreum. | johnson et al. (1993) showed that coexposure to uv-a between 300-400 nm enhanced the toxicity of nitrotoluenes to photobacterium phosphoreum, a marine bioluminescent bacteria used in the microtox test (microbics inc.). this paper reports that uv-a photoenhanced the toxicity of polynuclear aromatic hydrocarbons, other types of organic compounds, and some transition metals to p. phosphoreum. coexposure to 400 muw/cm2 for 15 min increased the toxicity of psoralen, alpha-terthienyl, anthracene, acri ... | 1994 | 7946004 |
| expression and properties of the recombinant lumazine (riboflavin) protein from photobacterium leiognathi. | photobacterium leiognathi lumazine protein has been expressed in escherichia coli in high yield, 30 mg/l. the cloned gene was one previously reported by illarionov (embl x56534), that had a similar sequence and was located in the same position as the lumazine protein gene in p. phosphoreum. this gene was placed downstream of the t7 gene 10 promoter of the plasmid pt7-7. when the e. coli are grown at 37 degrees c the protein accumulates in inclusion bodies but solubilization can be achieved in 6 ... | 1994 | 7947939 |
| modelling of human acute toxicity from physicochemical properties and non-vertebrate acute toxicity of the 38 organic chemicals of the meic priority list by pls regression and neural network. | linear and non-linear modelling of human acute toxicity (as human lethal concentrations; hlcs) of the 38 organic chemicals from the 50 priority compounds of the multicentre evaluation of in vitro cytotoxicity (meic) programme was investigated. the models obtained were derived either from a set of 23 physicochemical properties of the compounds or from their acute toxicities to five aquatic non-vertebrates together with the physicochemical properties. for the linear type, modelling was performed u ... | 1994 | 7959448 |
| the ctfa evaluation of alternatives program: an evaluation of in vitro alternatives to the draize primary eye irritation test. (phase ii) oil/water emulsions. | the cosmetic, toiletry and fragrance association (ctfa) evaluation of alternatives program is an evaluation of the relationship between draize ocular safety test data and comparable data from a selection of in vitro tests. in phase ii, 18 representative oil/water-based personal-care formulations were subjected to the draize primary eye safety test and 30 in vitro assay protocols (14 different types of in vitro endpoints were evaluated; the remainder were protocol variations). correlation of in v ... | 1994 | 7959449 |
| (trifluoromethyl)lumazine derivatives as 19f nmr probes for lumazine protein. | lumazine protein acts as an electronic excited state transducer in bioluminescence of photobacterium species. the protein binds 6,7-dimethyl-8-(d-ribityl)lumazine (1) which serves as the fluorophore. this compound also serves as a biosynthetic precursor of riboflavin and is the substrate of the enzyme riboflavin synthase. this enzyme and lumazine protein show considerable sequence homology. the interaction of lumazine apoprotein with several trifluoromethyl analogs of 6,7-dimethyl-8-ribityllumaz ... | 1994 | 8011629 |
| inhibition of bacterial enzyme activity and luminescence by urban river sediments. | the toxicological and ecological effects of pollutants in urban river sediments were studied. the sediments were chemically or physically fractionated, using selective extractants to separate the effects of metal and organic contaminants, and subsequently tested for the inhibition of bacterial enzyme activity and luminescence. in many cases the enzyme activity of the sediment-dwelling bacteria was inhibited by metals. the variations in inhibition were attributed to differences in sediment comple ... | 1994 | 8016633 |
| the lumazine synthase/riboflavin synthase complex of bacillus subtilis. x-ray structure analysis of hollow reconstituted beta-subunit capsids. | the lumazine synthase/riboflavin synthase complex of bacillus subtilis consists of an icosahedral capsid of 60 beta subunits enclosing a triplet of alpha subunits. an x-ray structure of 0.32 nm resolution has been obtained for the icosahedral capsid of the native alpha 3 beta 60 complex [ladenstein, r., schneider, m., huber, r., bartunik, h. d., wilson, k., schott, k. & bacher, a. (1988) j. mol. biol. 203, 1045-1070]. beta subunits were isolated after denaturation of the alpha 3 beta 60 complex ... | 1994 | 8055941 |
| a bacterial toxicity assay performed with microplates, microluminometry and microtox reagent. | we have developed a procedure for undertaking a microtox-based test by coupling microplate and microluminometric technologies. sample dilutions are prepared in a 96-well polystyrene microplate kept at 15 degrees c, while the microtox reagent and diluent are placed in an opaque, microluminometry-compatible 96-well microplate also kept at 15 degrees c. exposure begins when sample aliquots are brought into contact with bacterial reagents in the opaque microplate. after specific exposure times (5, 1 ... | 1994 | 8068350 |
| preparation of p-flavin-bound and p-flavin-free luciferase and p-flavin-bound beta-subunit of luciferase from photobacterium phosphoreum. | p-flavin-bound luciferase, p-flavin-free luciferase, and p-flavin-bound beta-subunit of luciferase were prepared from photobacterium phosphoreum using hydrophobic interaction chromatography after conventional purification using deae-cellulose chromatography and gel-filtration. the p-flavin-bound luciferase preparation contained about 20% p-flavin-free luciferase not removable by the present procedure. since the specific activity of the p-flavin-bound luciferase preparation was about 20% of that ... | 1994 | 8089082 |
| the use of the microtox system for evaluation of toxicity of the wastes in vítkovice steelworks, ostrava (czechoslovakia). | ostrava's industrial agglomeration is one of the most polluted areas in czechoslovakia due to an enormous production of wastes and wastewaters from both industry and municipal sources. as most of the wastes are deposited in the environment in a very simple way (usually in landfills) they may cause negative environmental effects and represent a serious hazardous factor for the surroundings. complex legislative regulations on waste treatment with respect to environmental protection are currently a ... | 1993 | 8108704 |
| an essential histidine residue required for fatty acylation and acyl transfer by myristoyltransferase from luminescent bacteria. | the lux-specific acyltransferases are serine esterases responsible for preferential diversion of myristic acid from fatty acid biosynthesis to the luminescent system. in contrast to other acyltransferases, an acylated enzyme intermediate can readily be detected making it ideal for the study of the mechanism of acyl transfer. although the transferase readily cleaves acyl carrier protein and acyl-coa, an alternate more rapid and convenient assay involving the cleavage of p-nitrophenyl acyl esters ... | 1994 | 8120025 |
| human acute toxicity prediction of the first 50 meic chemicals by a battery of ecotoxicological tests and physicochemical properties. | five acute bioassays consisting of three cyst-based tests (with artemia salina, streptocephalus proboscideus and brachionus calyciflorus), the daphnia magna test and the bacterial luminescence inhibition test (photobacterium phosphoreum) are used to determine the acute toxicity of the 50 priority chemicals of the multicentre evaluation of in vitro cytotoxicity (meic) programme. these tests and five physiocochemical properties (n-octanol-water partition coefficient, molecular weight, melting poin ... | 1994 | 8132177 |
| riboflavin synthesis genes are linked with the lux operon of photobacterium phosphoreum. | four genes immediately downstream of luxg in the photobacterium phosphoreum lux operon (ribebha) have been sequenced and shown to be involved in riboflavin synthesis. sequence analyses and complementation of escherichia coli riboflavin auxotrophs showed that the gene products of ribb and riba are 3,4-dihydroxy-2-butanone 4-phosphate (dhbp) synthetase and gtp cyclohydrolase ii, respectively. by expression of p. phosphoreum ribe in e. coli using the bacteriophage t7 promoter-rna polymerase system, ... | 1994 | 8144477 |
| use of a reporter gene to follow high-pressure signal transduction in the deep-sea bacterium photobacterium sp. strain ss9. | photobacterium sp. strain ss9 is a deep-sea bacterium which modulates the abundances of several outer membrane proteins as a function of hydrostatic pressure. these proteins include the product of the previously cloned omph gene (d. h. bartlett, m. wright, a. a. yayanos, and m. silverman. nature (london) 342:572-574, 1989). subsequent to conjugal plasmid delivery it was possible to cross an omph::lacz transcriptional fusion into the genome of ss9, replacing the wild-type omph gene, generating st ... | 1993 | 8244922 |
| spoilage and shelf-life of cod fillets packed in vacuum or modified atmospheres. | microbial growth, sensory and chemical changes and composition of gas atmosphere were studied in vacuum packed (vp) and modified atmosphere packed (map) cod fillets stored at 0 degree c. contrary to previous studies, coccobacilli and pleomorphic gram-negative microorganisms (2-4 by 2-5 microns) and not shewanella putrefaciens were found most likely to be the main spoilage organisms. these microorganisms, which may be photobacterium phosphoreum, can explain the short shelf-life extension of vp an ... | 1993 | 8257657 |
| growth and luminescence of luminous bacteria promoted by agents of microbial origin. | the examination of four species of luminous bacteria photobacterium leiognathi, photobacterium phosphoreum, vibrio fischeri and vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. these agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and ... | 1993 | 8285107 |
| an upper limit for the effect of 60 hz magnetic fields on bioluminescence from the photobacterium vibrio fischeri. | bioluminescence from vibrio fischeri was measured in the presence and absence of 60 hz magnetic fields. the peak value of the field was approximately 1.3 mt, a value approximately 13 times the earth's background static field and comparable to the ac field near heavy-duty electrical equipment such as generators. the objective of this work was a search for causality between the applied magnetic field and a basic biological function at the biochemical, membrane or cellular level based on the direct ... | 1994 | 8292047 |
| covalent immobilization of microorganisms in polymeric hydrogels. | a method of covalent immobilization of microorganisms (marine luminescent bacteria and yeast) in polymeric hydrogels is described. it is shown that cell immobilization leads to the creation of materials having properties of both synthetic polymers and physiologically active systems. application of systems containing covalent immobilized yeast and photobacteria in biotechnological and other processes is proposed. | 1993 | 8297830 |
| lumazine protein and the excitation mechanism in bacterial bioluminescence. | the spectral properties of lumazine protein and mixtures with the intermediates of the bacterial luciferase reaction, are reviewed. measurements of fluorescence dynamics in particular have been employed with the aim of elucidating the mechanism by which lumazine protein functions in the bioluminescence of the bacteria of the type photobacterium. the reaction of bacterial luciferase with its substrates produces bioluminescence emission with a spectral maximum at 496 nm. this spectrum is the same ... | 1993 | 8298053 |
| light organ symbioses in fishes. | most bioluminescent fishes are self-luminescent, but a substantial minority of bioluminescent teleosts produce light that is due to symbiotic luminous bacteria housed in elaborate light organs. the majority of symbiotically bioluminescent fishes (ten families in five orders) harbors common free-living species of marine luminous bacteria: photobacterium phosphoreum, p. leiognathi, and p. fischeri (= vibrio fischeri). others, associated with the beryciform family anomalopidae and nine families in ... | 1993 | 8305135 |
| sequence of the omph gene from the deep-sea bacterium photobacterium ss9. | in contrast to studies of many other extremophiles, the molecular characterization of the barophilic or high-pressure-adapted bacteria of the deep ocean is virtually nonexistent. one exception is the discovery that the moderate barophile photobacterium ss9 preferentially synthesizes a 37-kda outer membrane protein, designated omph, in response to elevated hydrostatic pressure. we report here on the molecular characterization of the omph gene. the deduced amino acid sequence of mature omph is sim ... | 1993 | 8396546 |
| use of the bioluminescent bacterium photobacterium phosphoreum to detect potentially biohazardous materials in water. | 1993 | 8400656 | |
| inhibitory effects of tannins on nadh dehydrogenases of various organisms. | we examined the effects of purified tannins and related compounds (33 species) on nadh-ubiquinone-1 oxidoreductase activity in four kinds of organism (paracoccus denitrificans, bacillus subtilis, photobacterium phosphoreum, and thermus thermophilus hb-8) and rat liver mitochondria. in addition to pentagalloylglucose, which was reported as a potent inhibitor of nadh dehydrogenases (ndh), sanguiin h-11, oolonghomobisflavan a, and polymerized procyanidin are potent inhibitors for both types of ndh ... | 1993 | 8401410 |
| mechanism of bacterial bioluminescence: 4a,5-dihydroflavin analogs as models for luciferase hydroperoxide intermediates and the effect of substituents at the 8-position of flavin on luciferase kinetics. | bioluminescence catalyzed by bacterial luciferases was measured using fmn, iso-fmn (6-methyl-8-nor-fmn), and fmn analogs carrying the following substituents at position 8: -h, -cl, -f, sme, some, -so2me, or -ome. the first-order rate constants for the decay of light emission correlate with the one-electron oxidation potentials of the 4a,5-dihydro forms of the fmn analogs. to determine the values of these potentials, isoalloxazine (flavin) derivatives having the 4a,5-propano-4a,5-dihydro structur ... | 1993 | 8422349 |
| toxicity of produced water from crude oil terminals to photobacterium phosphoreum, chaetoceros sp., and donax faba. | 1993 | 8428121 | |
| nucleotide sequence of the luxc gene encoding fatty acid reductase of the lux operon from photobacterium leiognathi. | the nucleotide sequence of the luxc gene (embl accession no. 65156) encoding fatty acid reductase (far) of the lux operon from photobacterium leiognathi pl741 was determined and the encoded amino acid sequence deduced. the fatty acid reductase is a component of the fatty acid reductase complex. the complex is responsible for converting fatty acid to aldehyde which serves as the substrate in the luciferase-catalyzed bioluminescent reaction. the protein comprises 478 amino acid residues and has a ... | 1993 | 8447834 |
| the lumazine protein-encoding gene in photobacterium leiognathi is linked to the lux operon. | the nucleotide (nt) sequence of the lump (embl accession no. x65612) gene of photobacterium leiognathi pl741 was determined and the amino acid (aa) sequence deduced. the encoded aa sequence of lump was identified as that of the lumazine protein (lump) by homology with that of photobacterium phosphoreum (56%). this small protein has a calculated m(r) of 19,997 and comprises 186 aa residues. biochemical studies suggested that lump is the protein which, when combined with luciferase, is responsible ... | 1993 | 8472956 |
| sequence of the luxd gene encoding acyltransferase of the lux operon from photobacterium leiognathi. | the nucleotide sequence of luxd (embl accession no. x65611), encoding acyltransferase (act), of the lux operon from photobacterium leiognathi pl741 was determined, and the amino acid (aa) sequence was deduced. act is a component of the fatty acid reductase complex, which is responsible for converting fatty acid to aldehyde that serves as the substrate in the luciferase-catalyzed bioluminescent reactions. the protein has a calculated m(r) of 34,384 and comprises 305 aa residues. alignment and com ... | 1993 | 8472957 |
| separation of ph, dilution, ionic strength and chemical matrix effects for biological monitoring of urines with the microtox test using nicotine, cotinine and reference urines. | the aim was to investigate the factors influencing light emission from photobacterium phosphoreum in the microtox test to interpret bioassay results for urine. four reference urines were assessed as reference materials for the bioassay. nicotine and cotinine were investigated as urinary markers for tobacco exposure. the optimum luminescence conditions were: 1.85%-3.25% nacl, 0.33-0.58 mol/l ionic strength, and ph 5.8-6.7. low ph values and high concentration of toxic trace metals were important ... | 1993 | 8475782 |
| crystal structure of a flavoprotein related to the subunits of bacterial luciferase. | the molecular structure of the luxf protein from the bioluminescent bacterium photobacterium leiognathi has been determined by x-ray diffraction techniques and refined to a conventional r-factor of 17.8% at 2.3 a resolution. the 228 amino acid polypeptide exists as a symmetrical homodimer and 33% of the monomer's solvent-accessible surface area is buried upon dimerization. the monomer displays a novel fold that contains a central seven-stranded beta-barrel. the solvent-exposed surface of the mon ... | 1993 | 8491169 |
| microbial bioassays to assess the toxicity of solid-associated contaminants. | due to the effects that sediment or soil matrices have on the bioavailability of compounds, it has been difficult to screen toxicity of solid-associated contaminants. the majority of microbial assays for testing toxicity of soils and sediments have been performed on water or solvent extracts. these procedures lead to a fractionation of the toxicity, which may underestimate or overestimate exposure routes and consequently potential adverse environmental effects. recently, a solid-phase microtox a ... | 1995 | 8521786 |
| toxicity of methyl tertiary butyl ether to daphnia magna and photobacterium phosphoreum. | 1995 | 8555689 | |
| three-dimensional model of the alpha-subunit of bacterial luciferase. | the predicted secondary structure of both subunits of bacterial luciferase is in accordance with a regular 8-fold alpha/beta-barrel structure. the 3d profile confirmed that luciferase subunits are compatible with the alpha/beta-barrel despite the absence of sequence similarity with any alpha/beta-barrel protein. the three-dimensional structure of 260 residues of the alpha-chain of luciferase was modeled from coordinates of glycolate oxidase and then energy minimized. the model obtained satisfies ... | 1995 | 8592705 |
| characterization of the genes coding for the putative sigma factor algu and its regulators muca, mucb, mucc, and mucd in azotobacter vinelandii and evaluation of their roles in alginate biosynthesis. | the study of the biosynthesis of alginate, the exopolysaccharide produced by azotobacter vinelandii and pseudomonas aeruginosa, has biotechnological and medical significance. we report here the identification of the a. vinelandii genes coding for the putative sigma factor algu and its negative regulators muca and mucb through the suppression of the highly mucoid phenotype of an a. vinelandii strain by a plasmid encoding muca and mucb. the sequences of the a. vinelandii algu, muca, and mucb genes ... | 1996 | 8606151 |
| regulatory region with puta gene of proline dehydrogenase that links to the lum and the lux operons in photobacterium leiognathi. | nucleotide sequence of regulatory region (r & r) with puta gene (embl accession no. u39227) from photobacterium leiognathi pl741 has been determined, and the puta gene encoded amino acid sequence of proline dehydrogenase is deduced. alignment and comparison of proline dehydrogenase of p. leiognathi with the proline dehydrogenase domain in the puta protein of escherichia coli and salmonella typhimurium show that they are homologous. nucleotide sequence reveals that regulatory region with the puta ... | 1996 | 8645272 |
| a pbrint family of plasmids for integration of cloned dna into the escherichia coli chromosome. | plasmid pbrint is an efficient vector for chromosomal integration of cloned dna into the lacz gene of escherichia coli [balbás et al., gene 136(1993) 211-213]. a family of related plasmids containing different antibiotic-resistance markers (cmr or gmr or kmr) and a larger multiple cloning site (mcs) has been constructed. this set of plasmids, whose integration efficiencies are as good as those obtained with the prototype plasmid pbrint, constitutes a collection of tools that allow rapid and easy ... | 1996 | 8654993 |
| applications of frontier molecular orbital energies in qsar studies. | 1996 | 8661859 | |
| quantitative structure-activity relationships of organic acids and bases. | 1996 | 8661905 | |
| direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from vibrio fischeri y1. | time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of vibrio fischeri, strain y1. in this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. on addition of the acceptor, the v. fischeri yellow fluorescence protein containing either fmn or riboflavin as ligand, a rapid d ... | 1996 | 8679599 |
| nmr analysis of site-specific ligand binding in oligomeric proteins. dynamic studies on the interaction of riboflavin synthase with trifluoromethyl-substituted intermediates. | the binding of small ligands to symmetrical oligomeric proteins may lead to a number of different partially ligated intermediates but should finally yield a symmetrical fully ligated enzyme/ligand complex. in the case of the trimeric protein, riboflavin synthase, some ligands form an unexpected protein/ligand complex, even in the presence of a large excess of ligand. three different bound forms were observed by 19f nmr spectroscopy, and scatchard-type analysis suggested binding sites of similar ... | 1996 | 8703935 |
| [bioluminescent analysis of the sos-response of escherichia coli cells]. | we constructed a recombinant plasmid ppls-1 to estimate the level of sos response in escherichia coli by the bioluminescent method. a 6.7-kb promoterless operon of bioluminescence from photobacterium leiognathi was cloned into a pbr322 vector, in which its expression was controlled by the sos promoter of gene cda from a plasmid cold. the sequence between the 5'-terminal sph1 site of the operon and start codon atg of the luxc gene was shown to be 56 bp in length and had no effect on the level of ... | 1996 | 8723628 |
| isolation and characterization of the structural gene for ompl, a pressure-regulated porin-like protein from the deep-sea bacterium photobacterium species strain ss9. | transposon-directed cloning was used to isolate the ompl gene from the deep-sea bacterium photobacterium species strain ss9. the deduced amino acid sequence of ompl displays sequence homology to porin proteins from enteric bacteria. gene fusion and primer extension analyses indicate that ompl is transcriptionally regulated by pressure. | 1996 | 8759872 |
| methylenetetrahydrofolate dehydrogenase-cyclohydrolase from photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue. | the marine bioluminescent bacterium photobacterium phosphoreum expresses a bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase with dual cofactor specificity. an investigation of the kinetic parameters of the p. phosphoreum enzyme indicate that its utilization of dinucleotide cofactors shares similarities with the human mitochondrial dehydrogenase-cyclohydrolase. both enzymes exhibit dual cofactor specificity and the nad(+)-dependent dehydrogenase activities from both enzymes can ... | 1996 | 8765228 |