Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| nutritional requirements of some marine luminous bacteria. | 1971 | 5087892 | |
| quantitative analysis of the phospholipids of some marine bioluminescent bacteria. | 1971 | 5117252 | |
| binding site determination from kinetic data. reduced flavin mononucleotide binding to bacterial luciferase. | 1971 | 5135318 | |
| bacterial carotenoids. xxxvi. remarkable c 43 -carotenoid artefacts of cross-conjugated carotenals and new carotenoid glucosides from athiorhodaceae spp. | 1971 | 5157085 | |
| hybridization of bacterial luciferase with a variant produced by chemical modification. | 1971 | 5161030 | |
| functional differences of the nonidentical subunits of bacterial luciferase. properties of hybrids of native and chemically modified bacterial luciferase. | 1971 | 5161031 | |
| fatty acid composition of bacterial membrane and wall lipids. | 1966 | 5328938 | |
| reductive pentose cycle and formate assimilation in rhodopseudomonas palustris. | rhodopseudomonas palustris assimilated formate autotrophically as carbon dioxide and hydrogen arising from the activity of the formic hydrogenlyase system. kinetic analyses of cell suspensions pulse-labeled with (14)c-formate or (14)c-bicarbonate showed similar distributions of incorporated radioactivity. in both cases phosphate esters were the first assimilation products. ribulose diphosphate carboxylase, phosphoribose isomerase, and phosphoribulokinase, characteristic enzymes of the reductive ... | 1969 | 5354954 |
| structurally distinct bacterial luciferases. | 1969 | 5365778 | |
| [isolation and crystallization of enzymes connected with the oluminescence of marine bacteria]. | 1969 | 5374197 | |
| potency of anaesthetic agents measured by luminous bacteria. | 1970 | 5416316 | |
| bioluminescence and radiation response to photobacterium fischeri h-2. | 1970 | 5429845 | |
| effect of anaesthetics on emission of light by luminous bacteria. | 1970 | 5440051 | |
| light-induced bioluminescence. isolation and characterization of a specific protein involved in the absorption and delayed emission of light. | 1970 | 5450232 | |
| effects of anaesthetics on luminous bacteria. | 1970 | 5455148 | |
| insoluble bacterial luciferases: a new approach to some problems in bioluminescence. | 1970 | 5456966 | |
| bacterial bioluminescence. comparisons of bioluminescence emission spectra, the fluorescence of luciferase reaction mixtures, and the fluorescence of flavin cations. | 1970 | 5459543 | |
| cellular control of the synthesis and activity of the bacterial luminescent system. | in bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth. the luciferase gene appears to be completely inactive in a freshly inoculated culture; the pulse of preferential luciferase synthesis which occurs later is the consequence of its activation at the level of deoxyribonucleic acid transcription which is attributed to an effect of a "conditioning" of the medium by the growing ... | 1970 | 5473898 |
| mutant analysis and enzyme subunit complementation in bacterial bioluminescence in photobacterium fischeri. | chemical mutagens were used to obtain mutants deficient in bioluminescence in the marine bacterium photobacterium fischeri strain mav. acridine dyes were effective in the production of dark mutants but not in the production of auxotrophs. these dark mutants were all of one type and appeared to contain lesions blocking the synthesis of luciferase. icr-191 was especially effective in the production of aldehyde mutants, i.e., dark strains that luminesce when a long-chain aldehyde such as n-decanal ... | 1970 | 5479452 |
| subunit homologies in bacterial luciferases. | 1970 | 5480160 | |
| response of photobacterium fischeri mav exposed to an ultra high dose-rate pulsed electron beam. | 1971 | 5539709 | |
| identification of n-nonaldehyde in photobacterium fisheri. | 1971 | 5557220 | |
| studies on luciferase from photobacterium phosphoreum. i. purification and physiochemical properties. | 1971 | 5562345 | |
| [study on a photobacterium isolated from the light organ of the leiognathidae fish]. | 1967 | 5624740 | |
| aspects of light production by photobacterium fischeri. | studies of luminescence in growing cultures of photobacterium fischeri revealed the characteristic kinetics of light emission, including a minimal phase of bacterial light output. a dialyzable substance present in the nutrient broth medium caused this transient inhibition in light production, although this substance did not affect culture growth. experiments were carried out to determine the mechanism of action and the chemical properties of the inhibitor. the results suggest that the inhibitor ... | 1968 | 5643069 |
| on the function of aldehyde in bacterial bioluminescence: evidence for an aldehyde requirement during luminescence from the frozen state. | 1968 | 5666729 | |
| the effect of anaesthetic agents on the emission of light by luminous bacteria. | 1969 | 5774528 | |
| bioluminescence as an in vivo index of radiation damage. | 1969 | 5777118 | |
| tryptophanase in diverse bacterial species. | the distribution of tryptophanase was studied. the highest observed specific activity, mumoles per minute per milligram (dry weight) cells, is given in parentheses after each species. tryptophanase was inducible and repressible in escherichia coli (.914), paracolobactrum coliforme (.210), proteus vulgaris (.146), aeromonas liquefaciens (.030), photobacterium harveyi (.035), sphaerophorus varius (.021), bacteroides sp. (.048), and corynebacterium acnes (.042). the enzyme was constitutive and nonr ... | 1969 | 5781572 |
| measuring the effect of air pollutants on bacterial luminescence: a simplified procedure. | 1969 | 5797990 | |
| the polarity of proton translocation in some photosynthetic microorganisms. | 1969 | 5802881 | |
| nutrition and metabolism of marine bacteria. xv. relation of na+-activated transport to the na+ requirement of a marine pseudomonad for growth. | drapeau, gabriel r., (mcgill university, montreal, quebec, canada), tibor i. matula, and robert a. macleod. nutrition and metabolism of marine bacteria. xv. relation of na(+)-activated transport to the na(+) requirement of a marine pseudomonad for growth. j. bacteriol. 92:63-71. 1966.-a marine pseudomonad was found to require 50 to 100 mm na(+) for maximal rate of oxidation of d-galactose and for the transport of d-fucose-h(3) into the cells. the same organism required 150 to 200 mm na(+) for th ... | 1966 | 5941284 |
| [species composition and some properties of marine periphyton bacteria isolated from species-specifically stained surfaces]. | 1966 | 6002796 | |
| the role of oxygen in the photoexcited luminescence of bacterial luciferase. | 1967 | 6017740 | |
| a comparative study of carbon source requirements of mycobacterium avium, group ii scotochromogens, group 3 nonphotochromogens, and mycobacterium terrae in the presence of glutamate-nitrogen. | 1967 | 6039105 | |
| an analysis of the relations between fluorescence and photochemistry during photosynthesis. | 1967 | 6040858 | |
| [bioluminescence and its analytic applications]. | 1983 | 6146159 | |
| generation of fatty acids by an acyl esterase in the bioluminescent system of photobacterium phosphoreum. | the fatty acid reductase complex from photobacterium phosphoreum has been discovered to have a long chain ester hydrolase activity associated with the 34k protein component of the complex. this protein has been resolved from the other components (50k and 58k) of the fatty acid reductase complex with a purity of greater than 95% and found to catalyze the transfer of acyl groups from acyl-coa primarily to thiol acceptors with a low level of transfer to glycerol and water. addition of the 50k prote ... | 1984 | 6147345 |
| proteolytic inactivation of luciferases from three species of luminous marine bacteria, beneckea harveyi, photobacterium fischeri, and photobacterium phosphoreum: evidence of a conserved structural feature. | upon limited proteolysis of luciferases from the luminous marine bacteria photobacterium fischeri, photobacterium phosphoreum, and beneckea harveyi, the rate of loss of luciferase activity is the same as the rate of loss of the heavier subunit of all three enzymes. it thus appears that the larger subunit of the luciferase from p. phosphoreum should be designated alpha based on its apparent homology with the alpha subunits of the luciferases from b. harveyi and p. fischeri. the luciferase from b. ... | 1980 | 6161366 |
| the ribonucleotide sequence of 5s rrna from two strains of deep-sea barophilic bacteria. | deep-sea bacteria were isolated from the digestive tract of animals inhabiting depths of 5900 m in the puerto rico trench and 4300 m near the walvis ridge. growth of two bacterial strains was measured in marine broth and in solid media under a range of pressures and temperatures. both strains were barophilic at 2 degrees c (+/- 1 degrees c) with an optimal growth rate of 0.22 h-1 at a pressure 30% lower than that encountered in situ. at 1 atm they grew at temperatures ranging from 1.2 to 18.2 de ... | 1984 | 6206199 |
| [effect of camp on the growth and development of luminescence in photobacterium belozerskii]. | the effect of camp on the parameters characterizing the development of luminescence in photobacterium belozerskii is discussed. an addition of camp to the culture medium shortened the latent time of luminescence development by 3--6 hours. the intensity of bacterial luminescence increased with a varying rate. during luminescence enhancement the rate of luciferase synthesis increased by a factor of 10 to 10(3). the rate of luciferase synthesis and the maxiumum level of bacterial luminescence when ... | 1980 | 6253982 |
| superoxide dismutases. | superoxide dismutases (ec 1.15.1.1) are metalloenzymes that catalytically scavenge the superoxide radical. they are essential for the aerobic survival of all forms of life. there are three types of superoxide dismutase, containing manganese, iron, or copper and zinc. the copper--zinc type has generally been isolated from eukaryotic cells except for the enzyme for the symbiotic marine bacterium photobacterium leiognathi. the copper--zinc type, from different sources, has a molecular weight of abo ... | 1980 | 6258885 |
| comparative biochemistry of the cell envelopes of photobacterium leiognathi and escherichia coli. | photobacterium leiognathi closely resembles escherichia coli with respect to cell lysis by lysozyme, and the fractionation of outer and cytoplasmic membranes. the two organisms differ in their phospholipid contents and, more significantly, in outer membrane protein compositions. | 1983 | 6352861 |
| [continuous cultivation of photobacterium phosphoreum luminescent bacteria with control of the luminescence]. | the paper describes a procedure for continuous cultivation of luminescent bacteria photobacterium phosphoreum according to which the nutrient flow is controlled with respect to the luminescence intensity. the biomass yield of this cultivation procedure at the given luminescence intensity 6.1 x 10(12) quantum x ml-1 s-1 is over three times higher than that obtained in periodic cultivation, with the specific cell luminescence being identical in both cases. the cultivation process is unstable at th ... | 1983 | 6353408 |
| evolutionary relationships in vibrio and photobacterium: a basis for a natural classification. | 1983 | 6357056 | |
| continuous-flow determination of primary bile acids, by bioluminescence, with use of nylon-immobilized bacterial enzymes. | we describe a continuous-flow bioluminescence method for measuring primary bile acid in serum, with nylon as the solid support. 7 alpha-hydroxysteroid dehydrogenase (ec 1.1.1. 159), a bacterial luciferase (ec 1.14.14.3), and nad+:fmn oxido-reductase (ec 1.6.8.1) are covalently co-immobilized on a nylon coil (1 m x 1.0 mm i.d.). the assay is highly specific for 7 alpha-hydroxy bile acids. other bile acids and steroids do not interfere. the continuous-flow light-emitting system, in which the react ... | 1984 | 6362913 |
| [metabolic organization of the luminescent system of phosphorescent bacteria]. | 1983 | 6418498 | |
| nucleotide base sequence of vibrionaceae 5 s rrna. | nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species. | 1984 | 6479333 |
| the amino-acid sequence of copper/zinc superoxide dismutase from swordfish liver. comparison of copper/zinc superoxide dismutase sequences. | the amino acid sequence of copper/zinc superoxide dismutase from swordfish (xiphias gladius) liver has been determined by alignment of the tryptic peptides according to the known sequence of bovine erythrocyte copper/zinc superoxide dismutase. this alignment has resulted in the ligands to the copper (his-47, 49, 76 and 94) and the zinc (his-76, 85, 134 and asp-97) being conserved in all the copper/zinc superoxide dismutases sequenced so far. also conserved in the sequences are the cysteines form ... | 1984 | 6510412 |
| extracellular lumazine from aggregating dictyostelium discoideum cells. influence of ph on its fluorescence. | lumazine has been demonstrated to be one of the two main compounds responsible for the extracellular fluorescence linked with the aggregation ability of dictyostelium discoideum, strain ax-2. the other compound, also having lumazine properties, is, however, different from the 7-hydroxylumazine proposed previously. the influence of ph on the fluorescence of lumazine was studied. the possible use of extracellular pteridines as ph markers was stressed and a method for the determination of the amoun ... | 1984 | 6519644 |
| [lactate dehydrogenase activity and its isoenzyme in a system coupled with bacterial luciferase]. | properties of a coupled system "ldh-bacterial luciferase" were studied. the light-generating enzymatic system from luminescent bacteria photobacterium fisheri was used as an indicator of the dehydrogenase activity. kinetic parameters of the coupled system were studied using commercially available preparation of ldh and the enzyme from human blood plasma. the luminescent activity of bacterial preparation was shown to correlate with the activity of ldh and its isoenzymes within wide range of blood ... | 1984 | 6528536 |
| relationship between ion requirements for respiration and membrane transport in a marine bacterium. | intact cells of the marine bacterium alteromonas haloplanktis 214 oxidized nadh, added to the suspending medium, by a process which was stimulated by na+ or li+ but not k+. toluene-treated cells oxidized nadh at three times the rate of untreated cells by a mechanism activated by na+ but not by li+ or k+. in the latter reaction, k+ spared the requirement for na+. intact cells of a. haloplanktis oxidized ethanol by a mechanism stimulated by either na+ or li+. the uptake of alpha-aminoisobutyric ac ... | 1984 | 6690427 |
| differential acylation in vitro with tetradecanoyl coenzyme a and tetradecanoic acid (+atp) of three polypeptides shown to have induced synthesis in photobacterium phosphoreum. | acylation of extracts of photobacterium phosphoreum at different stages of growth with [3h]tetradecanoic acid (+atp) has shown that two polypeptides found in the fatty acid reductase complex, the fatty acid activating enzyme (50k) and the 34k polypeptide, were specifically labeled during induction of the luminescent system. an alternate method for in vitro acylation of polypeptides in the luminescent system was developed using tetradecanoyl-coa. both the 34k polypeptide and, to a lesser extent, ... | 1984 | 6693412 |
| bioluminescence procedures for the measurement of nad(p) dependent enzyme catalytic activities in submicrogram quantities of rabbit and human nephron structures. | reduced flavin mononucleotide dependent luciferase (ec 1.14.14.3) from photobacterium fischeri has been used to measure nad(p) dependent enzymes in submicrogram quantities of tissue homogenates and isolated structures of rabbit and human kidney. the procedure for measuring nad(p)h was optimized, with internal standardization, to give a linear constant signal between 1 and 100 pmol. this method was applied to the measurement of glucose-6-phosphate dehydrogenase (ec 1.1.1.49) and 3-hydroxybutyrate ... | 1984 | 6716053 |
| vibrio harveyi aldehyde dehydrogenase. partial reversal of aldehyde oxidation and its possible role in the reduction of fatty acids for the bioluminescence reaction. | vibrio harveyi aldehyde dehydrogenase, which catalyzes the oxidation of long chain aliphatic aldehydes to acids, has been discovered to have both acyl-coa reductase and thioesterase activities. tetradecanoyl-coa was reduced to tetradecanal in the presence of nad(p)h, as monitored by the stimulation of luciferase activity by the aldehyde product (acyl-coa reductase). in the absence of nadph, [3h]tetradecanoyl-coa was hydrolyzed to the hexane-soluble fatty acid (thioesterase). inhibition data with ... | 1984 | 6725283 |
| growth and luminescence of luminous bacteria on modified haneda's medium. | 1984 | 6727711 | |
| bioluminescent toxicity assay of synfuel by-product waters. | 1984 | 6733307 | |
| a new rapid and sensitive bioluminescence assay for antibiotics that inhibit protein synthesis. | a new sensitive, rapid and simple bioluminescence assay for antibiotics inhibiting protein synthesis is described. in this assay the ability of the tested antibiotic to inhibit the de novo synthesis of the enzymes participating in the bacterial luminescence system is determined by means of a dark variant of a luminous bacterium that undergoes prompt induction of the luminescence system with certain dna-intercalating agents. upon induction, the in vivo luminescence of the dark variant is increase ... | 1984 | 6746464 |
| chemical and biological properties of lipopolysaccharide from a marine bacterium, photobacterium phosphoreum pj-1. | the chemical and biological properties of the lipopolysaccharide (lps) isolated from a marine bacterium, photobacterium phosphoreum pj-1, were studied. this lps consists of 40.6% carbohydrate, 27.3% fatty acid, 0.2% 2-keto-3-deoxyoctonate (kdo) and other components. one characteristic of this lps is its small amount of kdo, the basic component of the usual lps. electrophoresis in sodium dodecylsulfate polyacrylamide gel revealed at least two staining bands for carbohydrates. these bands were con ... | 1982 | 6752664 |
| [use of the bio- and chemiluminescence in a clinical laboratory]. | 1981 | 6752924 | |
| evidence for a natural gene transfer from the ponyfish to its bioluminescent bacterial symbiont photobacter leiognathi. the close relationship between bacteriocuprein and the copper-zinc superoxide dismutase of teleost fishes. | 1981 | 6787049 | |
| oxygen affinities of the hydrogenosome-containing protozoa tritrichomonas foetus and dasytricha ruminantium, and two aerobic protozoa, determined by bacterial bioluminescence. | oxygen-dependent bioluminescence of photobacterium (vibrio) fischeri was used to measure oxygen affinities of four protozoa. the aerobic organisms acanthamoeba castellanii and tetrahymena pyriformis showed apparent km values for o2 of 0.42 and 2.43 microm respectively. the aerotolerant anaerobe tritrichomonas foetus, and the more strictly anaerobic rumen ciliate dasytricha ruminantium, both of which have hydrogenosomes, respired with apparent km values of 1.08 and 1.70 microm-o2. we conclude tha ... | 1982 | 6809887 |
| immunochemical comparisons among lipopolysaccharides from symbiotic luminous bacteria isolated from several luminous marine animals. | 1982 | 6820471 | |
| raman spectra of flavin bound in flavodoxins and in other flavoproteins. evidence for structural variations in the flavin-binding region. | the resonance coherent anti-stokes raman scattering (cars) spectra for a number of flavoproteins are found to be fingerprints for the particular type of flavoprotein. one group studied were the bacterial flavodoxins: desulfovibrio vulgaris, desulfovibrio desulfuricans, azotobacter vinelandii, megasphaera elsdenii, clostridium kluyverii and clostridium formicoaceticum. the other examples were the enzymes lactate monooxygenase and glucose oxidase. fmn complexed to vibrio harveyi luciferase, and a ... | 1983 | 6840072 |
| saturation behavior of the manganese-containing superoxide dismutase from paracoccus denitrificans. | a pulse radiolysis study of the mn-superoxide dismutase from paracoccus denitrificans has shown that, at concentration of 0(2)-. below 0.8 x 10(-4)m, the catalyzed dismutation of 0(2)-. is a first order reaction with regard to 0(2)-.. at concentration of 0(2)-. above 0.8 x 10(-4)m, the mn-superoxide dismutase is shown to catalyze superoxide dismutation with a mechanism which exhibits saturation kinetics. this behavior was previously found in the bovine cu/zn-superoxide dismutase and in the fe-su ... | 1983 | 6860328 |
| phagocytosis-induced mutagenesis in bacteria. | dark mutants of the luminous bacteria photobacterium fischeri reverted to hereditary stable luminescent forms when incubated with human polymorphonuclear neutrophils (pmn). the maximal mutagenic effect occurred during the first 15 min of phagocytosis, and was dependent on the phagocyte:bacterium ratio as well as on the integrity of the pmn cells. heat-killed phagocytes or disintegrated phagocytes did not show any mutagenic activity, whereas the supernatant of the phagocytosis reaction exerted mu ... | 1983 | 6866004 |
| the effect of radiation on bioluminescent bacteria: possible use of luminescent bacteria as a biological dosemeter. | 1983 | 6867115 | |
| nitrogen fixation as evidence for the reducing nature of the early biosphere. | probably the first nitrogen fixers were anaerobic, non-photosynthetic, bacteria, i.e. fermenters. during the evolution of n2 fixation they still needed nitrogen on the oxidation level of ammonia. because of the complexities in structure and function of nitrogenase this evolution must have required a long time. the photosynthetic and later the respiring bacteria inherited the capacity for n2 fixation from the fermenters, but the process did not change a great deal when it was taken over. because ... | 1983 | 6871385 |
| [pathway of synthesis of the aldehyde factor - a basic substrate of luciferase]. | the stimulation of luminescence and cell aldehyde factor during the growth of one-aldehyde-dependent mutant in the condition medium by other aldehyde-dependent mutants was studied. it was shown that the aldehyde factor is synthesized in five successive steps, thus suggesting the participation of five enzymes in aldehyde factor synthesis. the metabolite accumulation in the condition medium increases both the level of the aldehyde factor and that of luciferase. it is assumed that some precursors o ... | 1983 | 6882834 |
| [bioluminescence study of nad-dependent dehydrogenase and isoenzyme activity of human blood using soluble and immobilized bacterial luciferase]. | 1983 | 6884193 | |
| the primary structure of cu-zn superoxide dismutase from photobacterium leiognathi: evidence for a separate evolution of cu-zn superoxide dismutase in bacteria. | the complete amino-acid sequence of the copper-zinc superoxide dismutase of the photobacterium leiognathi was determined. the fragmentation strategy employed included cyanogen bromide cleavage at its methionine residues and the only tryptophan residue. the s-carboxymethylated chain was further cleaved by means of trypsin, in order to obtain overlapping fragments. for sequence determination automated solid or liquid-phase techniques of edman degradation were used. c-terminal amino acids of the en ... | 1983 | 6884993 |
| acridine dyes and other dna-intercalating agents induce the luminescence system of luminous bacteria and their dark variants. | acridine dyes and other dna-intercalating agents such as ethidium bromide, theophylline, and caffeine induce luminescence in dark variants (k variants) different luminous species of bacteria, as well as in their wild-type luminous cells, prior to induction. the increase in luminescence appears 10-20 min after addition of these agents and is inhibited by chloramphenicol or rifampicin. addition of these agents affects the synthesis of both luciferase and aldehyde-synthesizing enzymes. it is hypoth ... | 1981 | 6943543 |
| reaction kinetics in living systems. | in this report we treat reaction rates, equilibrium theory, and irreversible thermodynamics as different aspects of a single discipline. in biological reactions the rate is ultimately controlled by enzymes and other proteins of complex structure and high molecular weight. the needed formalism can be placed in one-to-one correspondence with appropriate electrical and mechanical networks. an enzyme molecule has zwitter ions anchored in the polypeptide chain, which enable it to distort the substrat ... | 1981 | 6946491 |
| the effects of phosphate on the structure and stability of the luciferases from beneckea harveyi, photobacterium fischeri, and photobacterium phosphoreum. | 1980 | 6967319 | |
| complementation of subunits from different bacterial luciferases. evidence for the role of the beta subunit in the bioluminescent mechanism. | complementation of the nonidentical subunits (alpha and beta) of luciferases isolated from two different bioluminescent strains, beneckea harveyi and photobacterium phosphoreum, has resulted in the formation of a functional hybrid luciferase (alpha h beta p) containing the alpha subunit from b. harveyi luciferase (alpha h) and the beta subunit from p. phosphoreum luciferase (beta p). the complementation was unidirectional; activity could not be restored by complementing the alpha subunit of p. p ... | 1980 | 6969259 |
| taxonomy and description of vibrio fluvialis sp. nov. (synonym group f vibrios, group ef6). | 1981 | 6971864 | |
| bioluminescence from single bacterial cells exhibits no oscillation. | since the usual measurements of light emission from marine bacteria involve many (10(6)-10(10)) cells, the question has often been raised as to whether or not the individual cell's luminescence is truly continuous. to investigate this question, we assembled a sensitive photo-counting system with computerized data acquisition. several luminous species were studied: beneckea harveyi, photobacterium belozerskii, p. fischeri, and p. leiognathi. isolated single cells gave count rates ranging from 2 t ... | 1980 | 6973368 |
| immunological properties of lipopolysaccharide from a marine bacterium, photobacterium phosphoreum pj-1. | 1982 | 6982391 | |
| isolation of bacterial luciferases by affinity chromatography on 2,2-diphenylpropylamine-sepharose: phosphate-mediated binding to an immobilized substrate analogue. | a covalently immobilized form of an inhibitor of bacterial luciferase, 2,2-diphenylpropylamine (d phi pa), was an effective affinity resin for purifying this enzyme from several distinct bacterial species. the inhibitor is competitive with the luciferase aldehyde substrate but enhances binding of the flavin substrate fmnh2 (reduced riboflavin 5'-phosphate); comparable binding interactions occur with luciferase, the immobilized inhibitor d phi pa-sepharose, and the substrates [holzman, t. f., & b ... | 1982 | 6983889 |
| a new, sensitive and simple bioluminescence test for mutagenic compounds. | a spontaneous dark variant of the luminous bacterium photobacterium leiognathi was isolated. the reversion frequency of this variant to genetic-hereditary luminescent cells is greatly increased by nanogram quantities of different base-substitution and frameshift agents. this makes it possible to detect mutagenic compounds at concentrations 100 times lower than that detected by the ames test. curing agents, such as acridine dyes, ethidium bromide and sodium dodecyl sulfate, are also very active i ... | 1980 | 6990233 |
| copper-zinc superoxide dismutase from caulobacter crescentus cb15. a novel bacteriocuprein form of the enzyme. | a bacteriocuprein is a copper- and zinc-containing superoxide dismutase isolated from a bacterium. until recently, the first and only documented bacteriocuprein was that from the marine bacterium photobacterium leiognathi, which lives symbiotically with leiognathid fishes. a new bacteriocuprein has been discovered, purified, and characterized from the free living, non-symbiotic bacterium, caulobacter crescentus cb15. in its native molecular weight, homodimeric subunit structure, specific activit ... | 1982 | 7050107 |
| isolation and characterization of a protein with cyanide-sensitive superoxide dismutase activity from the prokaryote, paracoccus denitrificans. | 1. a protein with cyanide-sensitive superoxide dismutase activity was isolated from the prokaryote paracoccus denitrificans. 2. this enzyme, present in low amount in the cell, represented not more than 10% of the total cellular superoxide dismutase activity. it was obtained in a form which was 20-40-times less active than the main superoxide dismutase of p. denitrificans which is a manganese-containing enzyme. 3. it was a soluble monomeric enzyme, highly negatively charged (pi = 4.8), with an ap ... | 1982 | 7066332 |
| superoxide dismutases: exploration of apparent anomalies: a gene transfer and a functional replacement. | 1982 | 7067913 | |
| identification of vibrio hollisae sp. nov. from patients with diarrhea. | the name vibrio hollisae (synonym = special bacteriology group ef-13) is proposed for a new group of 16 strains that occurred in stool cultures of patients with diarrhea. v. hollisae is a small gram-negative rod, which is motile with a single polar flagellum. no lateral or peritrichous flagella were observed, even when it was grown on a solid medium. sodium chloride is required for growth, so v. hollisae is a halophilic vibrio. strains were positive (36 degrees c, 24 or 48 h) for oxidase (kovacs ... | 1982 | 7076812 |
| resolution of the fatty acid reductase from photobacterium phosphoreum into acyl protein synthetase and acyl-coa reductase activities. evidence for an enzyme complex. | 1982 | 7085612 | |
| association between lumazine protein and bacterial luciferase: direct demonstration from the decay of the lumazine emission anisotropy. | 1982 | 7093241 | |
| diluent composition for use of api 20e in characterizing marine and estuarine bacteria. | nine chemically defined inoculation diluents, with compositions ranging from 0.85% nacl to 35% marine salts, were used to evaluate the influence of diluent composition on the biochemical profiles of 30 marine and estuarine bacterial strains, including species of vibrio, aeromonas, allomonas, and photobacterium. results demonstrated that a 20% marine salts diluent enabled the characterization of halophilic strains normally nonreactive by the api 20e system. furthermore, the use of 20% marine salt ... | 1982 | 7125655 |
| [thermoinactivation of bacterial luciferase]. | the kinetics of thermal inactivation of bacterial luciferase was studied. it was found that the reaction substrates produce a weak protecting effect against the enzyme inactivation. edta, dithiothreitol and bovine serum albumin considerably stabilize the luciferase. the hypothetical mechanism of the enzyme inactivation involves oxidation of sh-groups and dissociation of the dimer into inactive monomers. | 1982 | 7150668 |
| [nadph- and atp-dependent luminescence of extracts from luminous bacteria]. | it was shown that the luminescence of extracts prepared from luminous bacteria is stimulated by nadph and atp without fmn or long-chain aliphatic aldehydes, which are routinely used for producing luminescence of extracts from luminous bacteria in vitro. in these extracts an aldehyde factor, a natural analog of aliphatic aldehydes, is synthesized. the enzymatic system involved in maintaining the luminescence of nadph and atp is probably not coupled with the functioning of nad(p)h: fmn oxidoreduct ... | 1982 | 7159622 |
| occurrence of uronic acid in lipopolysaccharides of vibrionaceae. | the occurrence of uronic acid as a sugar constituent of lipopolysaccharides (lps) in vibrionaceae was demonstrated for the first time. more than 100 strains were examined. of five genera constituting vibrionaceae, i.e., vibrio, aeromonas, plesiomonas, photobacterium, and lucibacterium, the latter three contained uronic acid in lps of all of their constituting members examined, while it was totally lacking in aeromonas lps so far tested. only the members of genus vibrio were found to be divided i ... | 1982 | 7169971 |
| sugar composition of lipopolysaccharides of family vibrionaceae. absence of 2-keto-3-deoxyoctonate (kdo) except in vibrio parahaemolyticus o6. | 1982 | 7176968 | |
| bacterial bioluminescence as a bioassay for mycotoxins. | the use of bacterial bioluminescence as a toxicological assay for mycotoxins was tested with rubratoxin b, zearalenone, penicillic acid, citrinin, ochratoxin a, pr-toxin, aflatoxin b1, and patulin. the concentrations of mycotoxins causing 50% light reduction (ec50) in photobacterium phosphoreum were determined immediately and at 5 h after reconstitution of the bacteria from a freeze-dried state. generally, less toxins were required to obtain an ec50 at 5 h. the effects of the above mycotoxins on ... | 1982 | 7181501 |
| [fatty acid makeup changes in the luminescent bacteria, photobacterium mandapamensis, in periodic cultivation]. | the fatty acid composition of the luminescent bacterium photobacterium mandapamensis and its spontaneous dark mutant was studied in dynamics. lipids of the both strains extracted with a methanol -- chloroform mixture contained the following fatty acids: lauric, tridecanoic, myristic, tetradecenic, pentadecanoic, pentadecenic, palmitic, palmitoleic, heptadecanoic, c17-cyclopropanoic, stearic, octadecenic, and nonadecanoic. the content of palmitoleic acid was the highest (57% of the total). not al ... | 1980 | 7207260 |
| [synthetic medium for photobacterium mandapamensis luminescent bacteria]. | 1980 | 7207266 | |
| [mechanism of action of 2,4-dinitrofluorobenzene on bacterial luminescence in vitro]. | 2,4-dinitrofluorobenzene (dnfb) changes the parameters of the bioluminescent reaction involving two enzymes, i.e. nadh: fmn-oxidoreductase and luciferase, by decreasing the maximal intensity of luminescence and increasing the time of maximal intensity. modification of the proteins does not affect the changes of the reaction parameters. the effects of dnfb on each one of the reactions, i.e. reduction of fnm and light emission, were studied. dnfb does not inhibit the reaction of fmn reduction. it ... | 1980 | 7213855 |
| [lipids of the luminescent bacteria photobacterium mandapamensis]. | the composition of lipids was studied in the luminescent bacterium photobacterium mandapamensis under the conditions of maximal luminescence. the synthesis of total lipids and poly-beta-hydroxybutyric acid (phba) was investigated in dynamics under the conditions of batch cultivation. the major class of lipids was polar lipids (84.3%) represented by phospholipids (phosphatidyl ethanolamine, phosphatidyl glycerol, cardiolipin, lysocardiolipin, lysophosphatidyl ethanolamine) and a minor nonidentifi ... | 1981 | 7219224 |
| [bacterial luciferase in reactions with catalytically reduced flavin mononucleotide]. | 1981 | 7225439 | |
| structural identification of autoinducer of photobacterium fischeri luciferase. | synthesis of bacterial luciferase in some strains of luminous bacteria requires a threshold concentration of an autoinducer synthesized by the bacteria and excreted into the medium. autoinducer excreted by photobacterium fischeri strain mj-1 was isolated from the cell-free medium by extraction with ethyl acetate, evaporation of solvent, workup with ethanol-water mixtures, and silica gel chromatography, followed by normal-phase and then reverse-phase high-performance liquid chromatography. the fi ... | 1981 | 7236614 |
| fluorescence emission spectra of cells and subcellular preparations of a green photosynthetic bacterium. effects of dithionite on the intensity of the emission bands. | fluorescence emission spectra were measured of intact cells and subcellular preparations of the green photosynthetic bacterium prosthecochloris aestuarii in the presence and in the absence of dithionite. a 3--5-fold increase in bacteriochlorophyll a fluorescence at 816 nm occurred upon addition of dithionite in a membrane vesicle preparation (complex i), in a photochemically active pigment-protein complex and in a bacteriochlorophyll a protein complex free from reaction centers. the pigment-prot ... | 1980 | 7236635 |
| [isolation and purification of bacterial luciferase from photobacterium fischeri for analytical purposes]. | a procedure resulting in a highly purified preparation of bacterial luciferase with a high specific activity towards fmnh2 and nadh was developed. using sds-electrophoresis in polyacrylamide gel, it was shown that the enzyme has a subunit composition and that its monomers do not contain the luciferase activity. the use of the obtained preparation for determining the content of nadh and fmn by the bioluminescent method allowed to increase its sensitivity up to 10(-18) and 10(-17) moles, respectiv ... | 1980 | 7248358 |