Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the molecular biology of barophilic bacteria. | many microorganisms from the deep-sea display high-pressure-adapted--also described as barophilic or piezophilic--growth characteristics. phylogenetic studies have revealed that a large proportion of the barophilic bacteria currently in culture collections belong to a distinct subgroup of the genus shewanella, referred to as the "barophile branch." many of the basic properties of barophiles that enable their survival at extremes of pressure remain to be elucidated. however, several genes whose e ... | 1997 | 9680316 |
| applications of frontier molecular orbital energies in qsar studies. | 1996 | 8661859 | |
| quantitative structure-activity relationships of organic acids and bases. | 1996 | 8661905 | |
| a selective and differential medium for vibrio harveyi. | a new medium, termed vibrio harveyi agar, has been developed for the isolation and enumeration of v. harveyi. it is possible to differentiate v. harveyi colonies from the colonies of strains representing 15 other vibrio species with this medium. this medium has been shown to inhibit the growth of two strains of marine pseudomonas spp. and two strains of marine flavobacterium spp. but to allow the growth of photobacterium strains. colonies displaying typical v. harveyi morphology were isolated fr ... | 1996 | 8795252 |
| interaction of photobacterium leiognathi and vibrio fischeri y1 luciferases with fluorescent (antenna) proteins: bioluminescence effects of the aliphatic additive. | the kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (lump) from photobacterium or the yellow fluorescence proteins (yfp) having fmn or rf bound, from vibrio fischeri strain y1. depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. these effects can be simply explained on the basis of the same pr ... | 1996 | 8810914 |
| methylenetetrahydrofolate dehydrogenase-cyclohydrolase from photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue. | the marine bioluminescent bacterium photobacterium phosphoreum expresses a bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase with dual cofactor specificity. an investigation of the kinetic parameters of the p. phosphoreum enzyme indicate that its utilization of dinucleotide cofactors shares similarities with the human mitochondrial dehydrogenase-cyclohydrolase. both enzymes exhibit dual cofactor specificity and the nad(+)-dependent dehydrogenase activities from both enzymes can ... | 1996 | 8765228 |
| direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from vibrio fischeri y1. | time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of vibrio fischeri, strain y1. in this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. on addition of the acceptor, the v. fischeri yellow fluorescence protein containing either fmn or riboflavin as ligand, a rapid d ... | 1996 | 8679599 |
| nmr analysis of site-specific ligand binding in oligomeric proteins. dynamic studies on the interaction of riboflavin synthase with trifluoromethyl-substituted intermediates. | the binding of small ligands to symmetrical oligomeric proteins may lead to a number of different partially ligated intermediates but should finally yield a symmetrical fully ligated enzyme/ligand complex. in the case of the trimeric protein, riboflavin synthase, some ligands form an unexpected protein/ligand complex, even in the presence of a large excess of ligand. three different bound forms were observed by 19f nmr spectroscopy, and scatchard-type analysis suggested binding sites of similar ... | 1996 | 8703935 |
| [bioluminescent analysis of the sos-response of escherichia coli cells]. | we constructed a recombinant plasmid ppls-1 to estimate the level of sos response in escherichia coli by the bioluminescent method. a 6.7-kb promoterless operon of bioluminescence from photobacterium leiognathi was cloned into a pbr322 vector, in which its expression was controlled by the sos promoter of gene cda from a plasmid cold. the sequence between the 5'-terminal sph1 site of the operon and start codon atg of the luxc gene was shown to be 56 bp in length and had no effect on the level of ... | 1996 | 8723628 |
| antigenicity and n-terminal amino acid sequence of a 35 kda porin-like protein of listonella (vibrio) anguillarum: comparison among different serotypes and other bacterial species. | listonella (vibrio) anguillarum, an important fish pathogen, is divided into 10 serotypes according to o-antigens present on the outer membrane. however, the biochemical and immunological properties of porin proteins have not been reported. in this study, the antigenicity and n-terminal amino acid sequence of the 35 kda porin-like-major outer membrane protein (omp35la) were compared among different serotypes of l. anguillarum as well as other bacteria. in western blotting analysis, antisera agai ... | 1996 | 8987710 |
| a bioluminescent gamma-aminobutyrate assay for monitoring its release from inhibitory nerve endings. | a bioluminescent gaba assay is described. the principle of the procedure is based on the action of gabase (gaba-aminotransferase plus succinic semialdehyde dehydrogenase), coupled to the detection of succinic semialdehyde and nadh, using photobacterium luciferase. the method was used for monitoring gaba release from depolarized brain slices. | 1996 | 8965088 |
| evaluation of the genotoxicity potential (by mutatox test) of ten pesticides found as water pollutants in cyprus. | ten pesticides: aldicarb, aldicard sulfone, aldicarb oxide, carbofuran, propoxur, methomyl, diuron, linuron, alachlor and parathion-methyl, found as water pollutants in cyprus, were evaluated for their genotoxicity potential with the mutatox test, both directly and after exogenous activation with s9 hepatic enzymes. a dark variant (m 169) of the photobacterium phosphoreum was used as the test organism. trials were undertaken in triplicate using ground-water spiked with pesticides solutions at te ... | 1996 | 9111768 |
| homogeneous bioluminescence competitive binding assay for folate based on a coupled glucose-6-phosphate dehydrogenase--bacterial luciferase enzyme system. | a homogeneous bioluminescence competitive binding assay for folate was developed by using a coupled enzyme system of glucose-6-phosphate dehydrogenase (g6pdh) and bacterial luciferase. a highly substituted g6pdh-folate conjugate was prepared by employing an n-hydroxysuccinimide/carbodiimide method. folate binding protein inhibits the activity of the conjugate. in the presence of folate, there is a competition between folate and the g6pdh-folate conjugate for the binding site of the folate bindin ... | 1996 | 8815749 |
| analysis of a pressure-regulated operon from the barophilic bacterium strain db6705. | a pressure-regulated operon was cloned and sequenced from a barophilic bacterium strain db6705. gene expression by this promote was regulated at the level of transcription by elevated pressure in the barophilic strain. the promoter sequence was similar to the promoter of omph from photobacterium sp. strain ss9. | 1996 | 8824840 |
| purification and characterization of a marine bacterial beta-galactoside alpha 2,6-sialyltransferase from photobacterium damsela jt0160. | a bacterial sialyltransferase, named sialyltransferase 0160, was purified from a marine bacterium that had been isolated from seawater from sagami bay, kanagawa. this strain has been identified as photobacterium damsela, and named p. damsela jt0160. sialyltransferase 0160 was purified 688-fold to homogeneity from the crude extract of the cells with a yield of 19% using a combination of anion exchange chromatography, hydroxyapatite chromatography, gel filtration chromatography, and affinity chrom ... | 1996 | 8864851 |
| identification of sodc encoding periplasmic [cu,zn]-superoxide dismutase in salmonella. | sodc, encoding [cu,zn]-cofactored superoxide dismutase, once thought to be virtually confined to eukaryotes, has now been described in many gram-negative pathogens that have their primary niche of colonization in the upper respiratory tract. their role in host-parasite interactive biology is unknown. we here show that members of the major human and animal enteric pathogenic species salmonella harbour a version of sodc most closely resembling that found in brucella abortus. the enzyme it encodes ... | 1996 | 8869506 |
| effect of modified atmosphere packaging on the tvb/tma-producing microflora of cod fillets. | cod fillets (gadus morhua) were packed under modified atmospheres, with four different gas compositions (60% co2-10% o2-30% n2, 60% co2-20% o2-20% n2, 60% co2-30% o2-10% n2, 60% co2-40% o2), and stored at 6 degrees c. plate counts were carried out after 3, 4, 5, 6 and 7 days, to follow the growth of aerobic and anaerobic bacteria, lactic acid bacteria, h2s-producing bacteria and enterobacteriaceae. the production of total volatile bases (tvb) and trimethylamine (tma), and the changes in ph of th ... | 1996 | 8880310 |
| flavin reductase p: structure of a dimeric enzyme that reduces flavin. | we report the structure of an nadph:fmn oxidoreductase (flavin reductase p) that is involved in bioluminescence by providing reduced fmn to luciferase. the 1.8 a crystal structure of flavin reductase p from vibrio harveyi was solved by multiple isomorphous replacement and reveals that the enzyme is a unique dimer of interlocking subunits, with 9352 a2 of surface area buried in the dimer interface. each subunit comprises two domains. the first domain consists of a four-stranded antiparallel beta- ... | 1996 | 8885832 |
| interspecific luciferase beta subunit hybrids between vibrio harveyi, vibrio fischeri and photobacterium leiognathi. | bacterial luciferase (ec 1.14.14.3) is a heterodimer composed of alpha- and beta-chains encoded by luxa and luxb, respectively. although some interspecific combinations of these subunits lead to active enzyme, others do not. the beta subunits of vibrio fischeri and photobacterium leiognathi form active enzyme with the alpha subunits of v.fischeri, p.leiognathi and vibrio harveyi, while the beta subunit from v.harveyi only complements the alpha subunit of v.harveyi. inactivity is caused by a lack ... | 1996 | 8888147 |
| microbiological spoilage of fish and fish products. | spoilage of fresh and lightly preserved fish products is caused by microbial action. this paper reviews the current knowledge in terms of the microbiology of fish and fish products with particular emphasis on identification of specific spoilage bacteria and the qualitative and quantitative biochemical indicators of spoilage. shewanella putrefaciens and pseudomonas spp. are the specific spoilage bacteria of iced fresh fish regardless of the origin of the fish. modified atmosphere stored marine fi ... | 1996 | 8913813 |
| novel dimeric interface and electrostatic recognition in bacterial cu,zn superoxide dismutase. | eukaryotic cu,zn superoxide dismutases (cuznsods) are antioxidant enzymes remarkable for their unusually stable beta-barrel fold and dimer assembly, diffusion-limited catalysis, and electrostatic guidance of their free radical substrate. point mutations of cuznsod cause the fatal human neurodegenerative disease amyotrophic lateral sclerosis. we determined and analyzed the first crystallographic structure (to our knowledge) for cuznsod from a prokaryote, photobacterium leiognathi, a luminescent s ... | 1996 | 8917495 |
| toxicological evaluation of harmful substances by in situ enzymatic and biological detection in high-performance thin-layer chromatography. | the efficiency of a chromatographic analysis method is determined by the selectivity of the chromatographic separation and the specificity of the detection method. in the case of high-performance thin-layer chromatography (hptlc) the separated components can be detected and quantified directly on the chromatogram by physical and chemical methods. by coupling high-performance thin-layer chromatography with biological or biochemical inhibition tests it was possible to detect toxcologically active ... | 1996 | 8938396 |
| nucleotide sequence and functional analysis of the luxe gene encoding acyl-protein synthetase of the lux operon from photobacterium leiognathi. | nucleotide sequence of the luxe gene genbank accession no. u66407 from photobacterium leiognathi pl741 has been determined, and the amino acid sequence of acyl-protein synthetase encoded by the luxe gene is deduced. nucleotide sequence reveals that the luxe gene encodes acyl-protein synthetase, which is a component of the fatty acid reductase complex that is responsible for converting fatty acid to aldehyde as substrate in the luciferase-catalyzed bioluminescence reaction. the acyl-protein synth ... | 1996 | 8941351 |
| characterization of the genes coding for the putative sigma factor algu and its regulators muca, mucb, mucc, and mucd in azotobacter vinelandii and evaluation of their roles in alginate biosynthesis. | the study of the biosynthesis of alginate, the exopolysaccharide produced by azotobacter vinelandii and pseudomonas aeruginosa, has biotechnological and medical significance. we report here the identification of the a. vinelandii genes coding for the putative sigma factor algu and its negative regulators muca and mucb through the suppression of the highly mucoid phenotype of an a. vinelandii strain by a plasmid encoding muca and mucb. the sequences of the a. vinelandii algu, muca, and mucb genes ... | 1996 | 8606151 |
| regulatory region with puta gene of proline dehydrogenase that links to the lum and the lux operons in photobacterium leiognathi. | nucleotide sequence of regulatory region (r & r) with puta gene (embl accession no. u39227) from photobacterium leiognathi pl741 has been determined, and the puta gene encoded amino acid sequence of proline dehydrogenase is deduced. alignment and comparison of proline dehydrogenase of p. leiognathi with the proline dehydrogenase domain in the puta protein of escherichia coli and salmonella typhimurium show that they are homologous. nucleotide sequence reveals that regulatory region with the puta ... | 1996 | 8645272 |
| a pbrint family of plasmids for integration of cloned dna into the escherichia coli chromosome. | plasmid pbrint is an efficient vector for chromosomal integration of cloned dna into the lacz gene of escherichia coli [balbás et al., gene 136(1993) 211-213]. a family of related plasmids containing different antibiotic-resistance markers (cmr or gmr or kmr) and a larger multiple cloning site (mcs) has been constructed. this set of plasmids, whose integration efficiencies are as good as those obtained with the prototype plasmid pbrint, constitutes a collection of tools that allow rapid and easy ... | 1996 | 8654993 |
| ecotoxicity monitoring--use of vibrio fischeri. | the proliferation of chemical substances having the potential to pollute any environmental medium (air, land, water), or humans via occupational exposure, la considerable. whilst chemical analytical techniques exist for the measurement of some of these chemicals, many of the methods involve costly techniques of considerable sophistication-quantification may be even more difficult. in less developed countries where sophisticated techniques may not be available or supplies of reagents, compressed ... | 1996 | 9127506 |
| isolation and characterization of the structural gene for ompl, a pressure-regulated porin-like protein from the deep-sea bacterium photobacterium species strain ss9. | transposon-directed cloning was used to isolate the ompl gene from the deep-sea bacterium photobacterium species strain ss9. the deduced amino acid sequence of ompl displays sequence homology to porin proteins from enteric bacteria. gene fusion and primer extension analyses indicate that ompl is transcriptionally regulated by pressure. | 1996 | 8759872 |
| structure of flavoprotein fp390 from a luminescent bacterium photobacterium phosphoreum refined at 2.7 a resolution. | the three-dimensional structure of a flavoprotein, fp(390), from a luminescent bacterium, photobacterium phosphoreum, solved by the molecular-replacement method, was refined to an r factor of 24.0% for 17 433 independent reflections, from 6.0 to 2.7 a resolution, collected by synchrotron radiation. the asymmetric unit of the crystal (space group p4(3)22, a = b = 76.8 and c = 242 a) contains two monomer molecules related by a non-crystallographic twofold axis to form a dimer. there are two q-flav ... | 1996 | 15299728 |
| interactions between aldehyde derivatives and the aldehyde binding site of bacterial luciferase. | the interaction of triazine aldehydes with the aldehyde binding site of bacterial luciferases was investigated using a series of triazine aldehydes with different aldehyde chain length, and substituents on the s-triazine ring. substrate activity was determined using luciferase from photobacterium fischeri and vibrio harveyi in a dithionite-based luciferases assay. the chain length optimum was determined for two triazine aldehyde classes to be c-10 and c-11, respectively. only the substrate activ ... | 1995 | 7762412 |
| structural refinement of the non-fluorescent flavoprotein from photobacterium leiognathi at 1.60 a resolution. | the crystallographically-determined structure of the non-fluorescent flavoprotein (nfp) from photobacterium leiognathi, a homolog of the bacterial luciferase subunits, has been refined to a conventional r-factor [formula: see text] of 0.175 using synchrotron data between 10.0 and 1.60 a resolution. the molecular structure is a homodimer of beta/alpha domains, the monomer having structural similarities to (beta alpha)8 barrel proteins. however, one beta-strand and three alpha-helices of a typical ... | 1995 | 7776372 |
| relative sensitivity of some selected aquatic organisms to phenol. | 1995 | 7780215 | |
| omph gene expression is regulated by multiple environmental cues in addition to high pressure in the deep-sea bacterium photobacterium species strain ss9. | photobacterium species strain ss9 is a moderately barophilic (pressure-loving) deep-sea bacterial species which induces the expression of the omph gene in response to elevated pressure. here we demonstrate that at 1 atm (1 atm = 1.01325 x 10(5) pa), omph expression increases with cell density in 2216 marine medium batch culture and is subject to catabolite repression and the omph synthesis is inducible by energy (carbon) starvation. regulatory mutants which are impaired in omph gene expression a ... | 1995 | 7860581 |
| small-subunit rrna sequences and whole dna relatedness concur for the reassignment of pasteurella piscicida (snieszko et al.) janssen and surgalla to the genus photobacterium as photobacterium damsela subsp. piscicida comb. nov. | the taxonomic status of pasteurella piscicida (strain ncimb 2058t [t = type strain] and a strain isolated from the environment) was investigated by performing phylogenetic analyses of small-subunit rrna sequences, dna-dna hybridization analyses, and biochemical characterization analyses. the results of the phylogenetic analyses and the levels of dna-dna complementarity demonstrated conclusively that pasteurella piscicida is extremely closely related to photobacterium damsela atcc 33539t. since t ... | 1995 | 7531996 |
| nucleotide sequence and functional analysis of regulatory region of the lump and the lux operon from photobacterium leiognathi. | the lump gene is linked to the lux operon, but runs in the opposite direction in photobacterium leiognathi pl741. the gene order of the lump and the lux operon is < -lump-r & r-luxc-luxd-luxa-luxb-luxn-luxe- > (r & r: regulatory region). the nucleotide sequence of the regulatory region (827-bp) between the lump and the lux operon was determined. sequence analysis illustrates that the regulatory region includes two divergent promoter systems, pr-promoter system for the lux operon (r-operon) and p ... | 1995 | 7763266 |
| a 26-kda outer membrane protein, ompk, common to vibrio species is the receptor for a broad-host-range vibriophage, kvp40. | kvp40 is a broad-host-range vibriophage forming plaques on strains of at least eight vibrio and one photobacterium species. a spontaneous kvp40-resistant mutant, r4000, derived from vibrio parahaemolyticus 1010 lacked a 26-kda outer membrane protein designated ompk. kvp40 was inactivated by outer membrane and ompk prepared from 1010, but not by outer membrane from r4000. these results strongly suggest that ompk is the receptor for kvp40. immunoblotting analyses using an anti-ompk rabbit serum re ... | 1995 | 7867914 |
| properties of recombinant fluorescent proteins from photobacterium leiognathi and their interaction with luciferase intermediates. | ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (embl x56534) of photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and sds-page. scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30 degrees c the kds (microm) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound fm ... | 1995 | 7880825 |
| ketone ec50 values in the microtox test. | the microtox ec50 values for the following ketones are reported in the following homologous series: straight chain methyl ketones (acetone, 2-butanone, 2-pentanone, 2-hepatonone, 2-octanone, 2-decanone, and 2-tridecanone); methyl ketones substituted at one alpha carbon (3-methyl-2-butanone; 3,3-dimethyl-2-butanone); methyl substituted at two alpha carbons (2,4-dimethyl-3-pentanone; 2,2,4,4-tetramethyl-3-pentanone); phenyl groups replacing methyl in acetone (acetophenone; benzophenone); methyl gr ... | 1995 | 7539364 |
| review of whole-organism bioassays: soil, freshwater sediment, and freshwater assessment in canada. | whole organism bioassays for the assessment of soil, freshwater sediment, and freshwater quality were evaluated for their application in the assessment and remediation of contaminated sites in canada under the national contaminated sites remediation program. using 3 essential and 12 desirable methodological criteria, bioassays were categorized as currently usable, prototype, or under development. based on further considerations related to bioassay application, a battery of usable screening and d ... | 1995 | 7541337 |
| acute aquatic toxicity of protolyzing substances studied as the microtox effect. | in tests of acute aquatic toxicity, the effects of protolyzing substances varies with ph. the microtox toxicity of six chlorophenols was studied over a ph interval and the relation of effects to dissociation was examined. the pka values of the chlorophenols under the optimal test conditions are presented. the results of the microtox toxicity tests supported a simple model of two species, phenol and phenate, with different specific toxicity. in five of the six chlorophenols only the effects of th ... | 1995 | 7541342 |
| relationship of aquatic natural organic material characteristics to the toxicity of selected insecticides. | toxicities of the commercial insecticide formulations of azinphos methyl, chlorpyrifos, fenvalerate, and methyl parathion were evaluated in streamwater samples containing natural organic material (nom) using a modification of a bacterial bioluminescence assay. toxicity reduction of azinphos methyl was significantly (p < 0.05) correlated with water sample nonvolatile total solids (nvts) concentration. toxicity reductions of fenvalerate and of methyl parathion were significantly correlated to e4/e ... | 1995 | 7544270 |
| the yellow bioluminescence bacterium, vibrio fischeri y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence fmn protein. | the yellow bioluminescence y1 strain of vibrio fischeri can produce a 22 kda protein with either fmn or riboflavin as a bound fluorophore. both forms are active for shifting the bioluminescence spectral maximum. the fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. the bioluminescence activity of the riboflavin y1 protein contrasts with the inactivity of the related photobacterium type. | 1995 | 7598706 |
| crystal structure determination of a flavoprotein fp390 from a luminescent bacterium, photobacterium phosphoreum. | the three-dimensional structure of a flavoprotein, fp390, purified from a luminescent bacterium, photobacterium phosphoreum, has been determined at 3 a resolution by x-ray crystallography. crystallographic refinements of the structural model have led to an r-factor of 0.24 for the intensity data between 6 to 3 a resolution collected with synchrotron radiation. it was found that a homodimer of the fp390 molecules related by a non-crystallographic 2-fold axis is comprised in the asymmetric unit. t ... | 1995 | 7629024 |
| assessment of rapid bioassays for detecting cyanobacterial toxicity. | simple and easy-to-use bioassays with artemia salina (brine shrimp) larvae, luminescent bacteria and pseudomonas putida were evaluated for the detection of toxicity due to cyanobacterial hepato- and neurotoxins. the hepatotoxins and a neurotoxin, anatoxin-a, were extracted from laboratory-grown cultures and natural bloom samples by the solid phase fractionation method and dissolved in diluent for different bioassays. the toxin concentration of cyanobacterial extracts was determined with hplc. th ... | 1995 | 7639991 |
| technical note: bioluminescent bacterial test for acute toxicity: the effect of ph and buffer solutions. | 1995 | 7640442 | |
| isolation of cdnas encoding gtp cyclohydrolase ii from arabidopsis thaliana. | a gtp cyclohydrolase ii-encoding gene from arabidopsis thaliana was isolated through functional complementation of a mutant of escherichia coli, bsv18, deficient in this protein. the derived amino-acid sequence constitutes a polypeptide of 27 kda and shows 37-58% identity with previously published sequences of escherichia coli, bacillus subtilis, photobacterium leiognathi and p. phosphoreum. | 1995 | 7642114 |
| quantitative structure-activity relationships as a tool to assess the comparative toxicity of organic chemicals. | quantitative structure-activity relationships of toxicity are discussed as a means of assessing the value of the microtox test which uses the light-emitting bacterium vibrio fisheri (photobacterium phosphoreum) as a replacement for toxicity testing in higher species. the microtox test is found to be a good surrogate for testing in fish, for compounds acting by the narcosis mechanism. however, for reactive chemicals the microtox test significantly underestimates the potential hazard. it should no ... | 1995 | 7670864 |
| formation of active bacterial luciferase between interspecific subunits in vivo. | interspecific complementation between luxas and luxbs from vibrio harveyi, vibrio fischeri, photobacterium leiognathi and xenorhabdus luminescens was examined in vivo. the individual genes from these species were cloned on different compatible plasmids or amplified by pcr and brought together to yield cis combinations without extraneous dna. the beta subunits from v. harveyi and x. luminescens form active enzyme only with alpha subunits from one of these species. all other combinations yield act ... | 1995 | 7676858 |
| the bacterial 'enigma': cracking the code of cell-cell communication. | in recent years it has become clear that the production of n-acyl homoserine lactones (n-ahls) is widespread in gram-negative bacteria. these molecules act as diffusible chemical communication signals (bacterial pheromones) which regulate diverse physiological processes including bioluminescence, antibiotic production, plasmid conjugal transfer and synthesis of exoenzyme virulence factors in plant and animal pathogens. the paradigm for n-ahl production is in the bioluminescence (lux) phenotype o ... | 1995 | 7476157 |
| biochemistry of bacterial bioluminescence. | 1995 | 7480148 | |
| modelling of microbial activity and prediction of shelf life for packed fresh fish. | prediction of shelf life based on growth of specific spoilage organisms (sso) in model substrates was studied. the effect of co2 on the growth kinetics for photobacterium phosphoreum and shewanella putrefaciens was quantified and modelled. results showed that microbial spoilage of packed cod stored with various concentrations of co2 was accurately predicted from the effect of co2 on p. phosphoreum grown in model substrates. the short shelf life extensions previously reported for packed cod there ... | 1995 | 7488526 |
| qualitative and quantitative characterization of spoilage bacteria from packed fish. | the large cells recently suggested to be responsible for spoilage of packed cod, have been identified as photobacterium phosphoreum. the spoilage activity of these cells, of shewanella putrefaciens and of other microorganisms isolated form spoiled packed cod has been studied. both qualitative and quantitative tests were used for characterization of the microbial spoilage activity. the importance of the different groups of microorganisms was evaluated by comparison of microbial spoilage activity ... | 1995 | 7488527 |
| characterisation of the yeni/yenr locus from yersinia enterocolitica mediating the synthesis of two n-acylhomoserine lactone signal molecules. | yersinia enterocolitica produces compounds capable of transcriptionally activating the photobacterium fischeri bioluminescence (lux) operon. using high-performance liquid chromatography, high resolution tandem mass spectrometry in conjunction with chemical synthesis, two signal molecules were identified and shown to be n-hexanoyl-l-homoserine lactone (hhl) and n-(3-oxohexanoyl)-l-homoserine lactone (ohhl). a gene (yeni) was isolated from y. enterocolitica and demonstrated to direct the synthesis ... | 1995 | 7494483 |
| the lumq gene is linked to the lump gene and the lux operon in photobacterium leiognathi. | the nucleotide sequence of the designated lumq gene (embl accession no. u35231) from photobacterium leiognathi pl741 has been determined, and the encoded amino acid sequence is deduced. the lumq protein has a calculated m(r) of 28,416 and comprises 248 amino acid residues. the lumq gene is identified as the envy-like gene by significant similarity of the encoded protein with the envy and adiy proteins of e. coli; there the envy gene encodes the porin thermoregulatory protein envy, and the adiy g ... | 1995 | 7503752 |
| an rpoe-like locus controls outer membrane protein synthesis and growth at cold temperatures and high pressures in the deep-sea bacterium photobacterium sp. strain ss9. | many deep-sea bacteria have evolved specialized adaptations for life at cold temperatures and high pressures. a locus required for both psychro- and baro-adaptation in the psychrophilic, moderate barophile, photobacterium species strain ss9 was identified among ss9 transposon mutants. dna sequence analysis of this locus identified four complete open reading frames (orfs), which appear to comprise an operon, and a fifth incomplete orf. all transposon insertions isolated are in orf3. extensive seq ... | 1995 | 8801425 |
| microbial bioassays to assess the toxicity of solid-associated contaminants. | due to the effects that sediment or soil matrices have on the bioavailability of compounds, it has been difficult to screen toxicity of solid-associated contaminants. the majority of microbial assays for testing toxicity of soils and sediments have been performed on water or solvent extracts. these procedures lead to a fractionation of the toxicity, which may underestimate or overestimate exposure routes and consequently potential adverse environmental effects. recently, a solid-phase microtox a ... | 1995 | 8521786 |
| toxicity of methyl tertiary butyl ether to daphnia magna and photobacterium phosphoreum. | 1995 | 8555689 | |
| three-dimensional model of the alpha-subunit of bacterial luciferase. | the predicted secondary structure of both subunits of bacterial luciferase is in accordance with a regular 8-fold alpha/beta-barrel structure. the 3d profile confirmed that luciferase subunits are compatible with the alpha/beta-barrel despite the absence of sequence similarity with any alpha/beta-barrel protein. the three-dimensional structure of 260 residues of the alpha-chain of luciferase was modeled from coordinates of glycolate oxidase and then energy minimized. the model obtained satisfies ... | 1995 | 8592705 |
| modelling of human acute toxicity from physicochemical properties and non-vertebrate acute toxicity of the 38 organic chemicals of the meic priority list by pls regression and neural network. | linear and non-linear modelling of human acute toxicity (as human lethal concentrations; hlcs) of the 38 organic chemicals from the 50 priority compounds of the multicentre evaluation of in vitro cytotoxicity (meic) programme was investigated. the models obtained were derived either from a set of 23 physicochemical properties of the compounds or from their acute toxicities to five aquatic non-vertebrates together with the physicochemical properties. for the linear type, modelling was performed u ... | 1994 | 7959448 |
| a bacterial toxicity assay performed with microplates, microluminometry and microtox reagent. | we have developed a procedure for undertaking a microtox-based test by coupling microplate and microluminometric technologies. sample dilutions are prepared in a 96-well polystyrene microplate kept at 15 degrees c, while the microtox reagent and diluent are placed in an opaque, microluminometry-compatible 96-well microplate also kept at 15 degrees c. exposure begins when sample aliquots are brought into contact with bacterial reagents in the opaque microplate. after specific exposure times (5, 1 ... | 1994 | 8068350 |
| phylogenetic analysis and assessment of the genera vibrio, photobacterium, aeromonas, and plesiomonas deduced from small-subunit rrna sequences. | we sequenced nearly complete small-subunit rrnas of 54 reference strains belonging to the genera vibrio, photobacterium, aeromonas, and plesiomonas. we then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony ... | 1994 | 7520733 |
| phototoxicology. 2. near-ultraviolet light enhancement of microtox assays of trinitrotoluene and aminodinitrotoluenes. | coexposure of 2,4,6-trinitrotoluene (tnt), 2-amino-4,6-dinitrotoluene (2a), or 4-amino-2,6-dinitrotoluene (4a) to near-ultraviolet (nuv) light (lambda max-354 nm) significantly enhanced their toxicity toward photobacterium phosphoreum (microtox bioassay) during 30 min but not 15 min. based on the slopes of the dose-response lines, the nuv coexposure and dark toxic mechanisms of action for tnt, 2a, and 4a appeared to be similar. nuv coexposure of binary mixtures significantly enhanced (supraaddit ... | 1994 | 7525202 |
| the environmental risks of industrial waste disposal: an experimental approach including acute and chronic toxicity studies. | the toxicity of 15 leachates of various solid industrial wastes accepted in an engineered landfill has been studied. a cost-effective battery of tests allowing evaluation of acute and chronic toxicity, as well as genotoxicity, and investigations on different trophic levels in the aquatic environment has been used. acute toxicity was tested on bacteria (microtox assay with photobacterium phosphoreum) and microcrustaceans (daphnia magna immobilization assay). a growth inhibition test of microalgae ... | 1994 | 7525226 |
| penaeid prawns and associated luminous bacteria. | luminous bacteria associated with the exoskeleton, gill and gut of penaeid prawns, penaeus indicus h. milne edwards and penaeus monodon fabricius in mangalore waters were isolated, identified and quantified. two species of bacteria, vibrio harveyi and vibrio fischeri were recorded, the former was dominant in the exoskeleton and gill of both the penaeids. gut of prawns supported exclusive and dense populations of v. harveyi suggesting that the species is well adapted to proliferate in this microe ... | 1994 | 7866726 |
| ecotox-evaluation strategy for soil bioremediation exemplified for a pah-contaminated site. | during a bioremediation of a pah-contaminated site chemical and biological analyses were carried out. the biological investigations included ecotoxicological analyses in the aqueous extract, (pseudomonas putida, photobacterium phosphoreum, daphnids, algae, fish) and analyses in the soil with introduced organisms (plants, earthworms) and natural soil organisms (nematodes, microorganisms). in all test systems a correspondence between decreasing toxicity and degradation of the easily biodegradable ... | 1994 | 7922149 |
| uv-a coexposure enhances the toxicity of aromatic hydrocarbons, munitions, and metals to photobacterium phosphoreum. | johnson et al. (1993) showed that coexposure to uv-a between 300-400 nm enhanced the toxicity of nitrotoluenes to photobacterium phosphoreum, a marine bioluminescent bacteria used in the microtox test (microbics inc.). this paper reports that uv-a photoenhanced the toxicity of polynuclear aromatic hydrocarbons, other types of organic compounds, and some transition metals to p. phosphoreum. coexposure to 400 muw/cm2 for 15 min increased the toxicity of psoralen, alpha-terthienyl, anthracene, acri ... | 1994 | 7946004 |
| expression and properties of the recombinant lumazine (riboflavin) protein from photobacterium leiognathi. | photobacterium leiognathi lumazine protein has been expressed in escherichia coli in high yield, 30 mg/l. the cloned gene was one previously reported by illarionov (embl x56534), that had a similar sequence and was located in the same position as the lumazine protein gene in p. phosphoreum. this gene was placed downstream of the t7 gene 10 promoter of the plasmid pt7-7. when the e. coli are grown at 37 degrees c the protein accumulates in inclusion bodies but solubilization can be achieved in 6 ... | 1994 | 7947939 |
| the ctfa evaluation of alternatives program: an evaluation of in vitro alternatives to the draize primary eye irritation test. (phase ii) oil/water emulsions. | the cosmetic, toiletry and fragrance association (ctfa) evaluation of alternatives program is an evaluation of the relationship between draize ocular safety test data and comparable data from a selection of in vitro tests. in phase ii, 18 representative oil/water-based personal-care formulations were subjected to the draize primary eye safety test and 30 in vitro assay protocols (14 different types of in vitro endpoints were evaluated; the remainder were protocol variations). correlation of in v ... | 1994 | 7959449 |
| (trifluoromethyl)lumazine derivatives as 19f nmr probes for lumazine protein. | lumazine protein acts as an electronic excited state transducer in bioluminescence of photobacterium species. the protein binds 6,7-dimethyl-8-(d-ribityl)lumazine (1) which serves as the fluorophore. this compound also serves as a biosynthetic precursor of riboflavin and is the substrate of the enzyme riboflavin synthase. this enzyme and lumazine protein show considerable sequence homology. the interaction of lumazine apoprotein with several trifluoromethyl analogs of 6,7-dimethyl-8-ribityllumaz ... | 1994 | 8011629 |
| inhibition of bacterial enzyme activity and luminescence by urban river sediments. | the toxicological and ecological effects of pollutants in urban river sediments were studied. the sediments were chemically or physically fractionated, using selective extractants to separate the effects of metal and organic contaminants, and subsequently tested for the inhibition of bacterial enzyme activity and luminescence. in many cases the enzyme activity of the sediment-dwelling bacteria was inhibited by metals. the variations in inhibition were attributed to differences in sediment comple ... | 1994 | 8016633 |
| the lumazine synthase/riboflavin synthase complex of bacillus subtilis. x-ray structure analysis of hollow reconstituted beta-subunit capsids. | the lumazine synthase/riboflavin synthase complex of bacillus subtilis consists of an icosahedral capsid of 60 beta subunits enclosing a triplet of alpha subunits. an x-ray structure of 0.32 nm resolution has been obtained for the icosahedral capsid of the native alpha 3 beta 60 complex [ladenstein, r., schneider, m., huber, r., bartunik, h. d., wilson, k., schott, k. & bacher, a. (1988) j. mol. biol. 203, 1045-1070]. beta subunits were isolated after denaturation of the alpha 3 beta 60 complex ... | 1994 | 8055941 |
| preparation of p-flavin-bound and p-flavin-free luciferase and p-flavin-bound beta-subunit of luciferase from photobacterium phosphoreum. | p-flavin-bound luciferase, p-flavin-free luciferase, and p-flavin-bound beta-subunit of luciferase were prepared from photobacterium phosphoreum using hydrophobic interaction chromatography after conventional purification using deae-cellulose chromatography and gel-filtration. the p-flavin-bound luciferase preparation contained about 20% p-flavin-free luciferase not removable by the present procedure. since the specific activity of the p-flavin-bound luciferase preparation was about 20% of that ... | 1994 | 8089082 |
| immobilized cells used for detection and analysis. | many kinds of biosensor have now been developed and utilized for various types of analysis including clinical, medical and environmental monitoring, industrial process control, as well as many other applications. the microbial biosensor has advantages, such as longer lifetime and lower cost, over other types of biosensor. recently, photobacteria and recombinant bacteria have been employed in biosensors both for determining biochemical oxygen demand and for detecting heavy metals and other toxic ... | 1994 | 7764644 |
| mechanism-based comparisons of acute toxicities elicited by industrial organic chemicals in procaryotic and eucaryotic systems. | comparisons of toxicities elicited by nonpolar and polar narcotics, weak acid uncouplers of oxidative phosphorylation, and bioreactive chemicals between the eucaryotic systems pimephales promelas and tetrahymena pyriformis and the procaryotic systems escherichia coli and photobacterium phosphoreum were performed. each chemical had been a priori assigned a mechanism/mode of action based on the results from previous studies with eucaryotic systems. hydrophobicity-dependent qsars for nonpolar narco ... | 1994 | 7533711 |
| human acute toxicity prediction of the first 50 meic chemicals by a battery of ecotoxicological tests and physicochemical properties. | five acute bioassays consisting of three cyst-based tests (with artemia salina, streptocephalus proboscideus and brachionus calyciflorus), the daphnia magna test and the bacterial luminescence inhibition test (photobacterium phosphoreum) are used to determine the acute toxicity of the 50 priority chemicals of the multicentre evaluation of in vitro cytotoxicity (meic) programme. these tests and five physiocochemical properties (n-octanol-water partition coefficient, molecular weight, melting poin ... | 1994 | 8132177 |
| riboflavin synthesis genes are linked with the lux operon of photobacterium phosphoreum. | four genes immediately downstream of luxg in the photobacterium phosphoreum lux operon (ribebha) have been sequenced and shown to be involved in riboflavin synthesis. sequence analyses and complementation of escherichia coli riboflavin auxotrophs showed that the gene products of ribb and riba are 3,4-dihydroxy-2-butanone 4-phosphate (dhbp) synthetase and gtp cyclohydrolase ii, respectively. by expression of p. phosphoreum ribe in e. coli using the bacteriophage t7 promoter-rna polymerase system, ... | 1994 | 8144477 |
| common structural features of the luxf protein and the subunits of bacterial luciferase: evidence for a (beta alpha)8 fold in luciferase. | the amino acid sequence identity and potential structural similarity between the subunits of bacterial luciferase and the recently determined structure of the luxf molecule are examined. the unique beta/alpha barrel fold found in luxf appears to be conserved in part in the luciferase subunits. from secondary structural predictions of both luciferase subunits, and from structural comparisons between the protein product of the luxf gene, nfp, and glycolate oxidase, we propose that it is feasible f ... | 1994 | 7703838 |
| an essential histidine residue required for fatty acylation and acyl transfer by myristoyltransferase from luminescent bacteria. | the lux-specific acyltransferases are serine esterases responsible for preferential diversion of myristic acid from fatty acid biosynthesis to the luminescent system. in contrast to other acyltransferases, an acylated enzyme intermediate can readily be detected making it ideal for the study of the mechanism of acyl transfer. although the transferase readily cleaves acyl carrier protein and acyl-coa, an alternate more rapid and convenient assay involving the cleavage of p-nitrophenyl acyl esters ... | 1994 | 8120025 |
| genetic characterization of omph mutants in the deep-sea bacterium photobacterium sp. strain ss9. | omph is an outer membrane protein produced by the deep-sea bacterium photobacterium species strain ss9 in response to elevated hydrostatic pressure. in order to facilitate studies of the function of this protein, a series of omph+ and omph- strains were obtained from ss9 by tn5 gene replacement mutagenesis. a previously isolated omph::lacz strain and a derivative of this strain harboring a plasmid expressing the wild-type omph gene were also utilized. the acridine mutagen icr 191 preferentially ... | 1994 | 7857197 |
| an upper limit for the effect of 60 hz magnetic fields on bioluminescence from the photobacterium vibrio fischeri. | bioluminescence from vibrio fischeri was measured in the presence and absence of 60 hz magnetic fields. the peak value of the field was approximately 1.3 mt, a value approximately 13 times the earth's background static field and comparable to the ac field near heavy-duty electrical equipment such as generators. the objective of this work was a search for causality between the applied magnetic field and a basic biological function at the biochemical, membrane or cellular level based on the direct ... | 1994 | 8292047 |
| [studies on fugu (globefish) tetrodotoxin over one century]. | one century has passed since fugu toxin was first named tetrodotoxin by tahara. chemical problems such as crystallization of tetrodotoxin, followed by structure determination by the research groups headed by tsuda, hirata, woodward, and mosher, and its subsequent synthesis by kishi have been solved. in the last 10 years, research on the distribution of tetrodotoxin in nature has shown that tetrodotoxin is not produced by fugu fish, but rather is formed by sea bacteria such as vibrio, alteromonas ... | 1994 | 11613510 |
| identification of vibrio splendidus as a member of the planktonic luminous bacteria from the persian gulf and kuwait region with luxa probes. | hybridization probes specific for the luxa genes of four groups of luminous bacteria were used to screen luminous isolates obtained from the persian gulf, near al khiran, kuwait nine of these isolates were identified as vibrio harveyi, a commonly encountered planktonic isolate, while three others showed no hybridization to any of the four probes (v. harveyi, vibrio fischeri, photobacterium phosphoreum, or photobacterium leiognathi) under high-stringency conditions. polymerase chain reaction ampl ... | 1993 | 16349023 |
| a novel bod sensor based on bacterial luminescence. | a reagent-type bod sensor with a new principle employing a luminous bacterium, photobacterium phosphoreum, was developed. the increased intensity of luminescence resulting from the cellular assimilation of organic compounds in wastewater was detected by a photodiode. the bod response of the bacterial reagent could be obtained within 15 min with +/-7% error. the temperature condition for optimal bod response was 18 degrees to 25 degrees c at ph 7 to 8, indicating that it is possible to measure bo ... | 1993 | 18601297 |
| covalent immobilization of microorganisms in polymeric hydrogels. | a method of covalent immobilization of microorganisms (marine luminescent bacteria and yeast) in polymeric hydrogels is described. it is shown that cell immobilization leads to the creation of materials having properties of both synthetic polymers and physiologically active systems. application of systems containing covalent immobilized yeast and photobacteria in biotechnological and other processes is proposed. | 1993 | 8297830 |
| lumazine protein and the excitation mechanism in bacterial bioluminescence. | the spectral properties of lumazine protein and mixtures with the intermediates of the bacterial luciferase reaction, are reviewed. measurements of fluorescence dynamics in particular have been employed with the aim of elucidating the mechanism by which lumazine protein functions in the bioluminescence of the bacteria of the type photobacterium. the reaction of bacterial luciferase with its substrates produces bioluminescence emission with a spectral maximum at 496 nm. this spectrum is the same ... | 1993 | 8298053 |
| sequence of the omph gene from the deep-sea bacterium photobacterium ss9. | in contrast to studies of many other extremophiles, the molecular characterization of the barophilic or high-pressure-adapted bacteria of the deep ocean is virtually nonexistent. one exception is the discovery that the moderate barophile photobacterium ss9 preferentially synthesizes a 37-kda outer membrane protein, designated omph, in response to elevated hydrostatic pressure. we report here on the molecular characterization of the omph gene. the deduced amino acid sequence of mature omph is sim ... | 1993 | 8396546 |
| use of a reporter gene to follow high-pressure signal transduction in the deep-sea bacterium photobacterium sp. strain ss9. | photobacterium sp. strain ss9 is a deep-sea bacterium which modulates the abundances of several outer membrane proteins as a function of hydrostatic pressure. these proteins include the product of the previously cloned omph gene (d. h. bartlett, m. wright, a. a. yayanos, and m. silverman. nature (london) 342:572-574, 1989). subsequent to conjugal plasmid delivery it was possible to cross an omph::lacz transcriptional fusion into the genome of ss9, replacing the wild-type omph gene, generating st ... | 1993 | 8244922 |
| spoilage and shelf-life of cod fillets packed in vacuum or modified atmospheres. | microbial growth, sensory and chemical changes and composition of gas atmosphere were studied in vacuum packed (vp) and modified atmosphere packed (map) cod fillets stored at 0 degree c. contrary to previous studies, coccobacilli and pleomorphic gram-negative microorganisms (2-4 by 2-5 microns) and not shewanella putrefaciens were found most likely to be the main spoilage organisms. these microorganisms, which may be photobacterium phosphoreum, can explain the short shelf-life extension of vp an ... | 1993 | 8257657 |
| light organ symbioses in fishes. | most bioluminescent fishes are self-luminescent, but a substantial minority of bioluminescent teleosts produce light that is due to symbiotic luminous bacteria housed in elaborate light organs. the majority of symbiotically bioluminescent fishes (ten families in five orders) harbors common free-living species of marine luminous bacteria: photobacterium phosphoreum, p. leiognathi, and p. fischeri (= vibrio fischeri). others, associated with the beryciform family anomalopidae and nine families in ... | 1993 | 8305135 |
| toxicity of sediments and sediment pore waters from the grand calumet river-indiana harbor, indiana area of concern. | the assessment of contaminated sediments is a difficult task due to the complex nature of the sediment matrix and the potential for exposure of aquatic organisms to in-place contaminants via several routes. differential species sensitivity also precludes the completion of a meaningful environmental assessment with only one species. therefore, a battery of assays approach with the microtox assay, 48 hr daphnia magna and ceriodaphnia dubia tests and a 10-day chironomous tentans test was used to ev ... | 1993 | 7691537 |
| the use of the microtox system for evaluation of toxicity of the wastes in vítkovice steelworks, ostrava (czechoslovakia). | ostrava's industrial agglomeration is one of the most polluted areas in czechoslovakia due to an enormous production of wastes and wastewaters from both industry and municipal sources. as most of the wastes are deposited in the environment in a very simple way (usually in landfills) they may cause negative environmental effects and represent a serious hazardous factor for the surroundings. complex legislative regulations on waste treatment with respect to environmental protection are currently a ... | 1993 | 8108704 |
| marine biology. light genes will out. | 1993 | 7683389 | |
| bioluminescent symbionts of flashlight fishes and deep-sea anglerfishes form unique lineages related to the genus vibrio. | bioluminescent symbioses range from facultative associations to highly adapted, apparently obligate ones. the family anomalopidae (flashlight fishes) encompasses five genera of tropical reef fishes that have large suborbital light organs. the suborder ceratioidei (deep-sea anglerfishes) contains 11 families. in nine of these, females have a bioluminescent lure that contains bacterial symbionts. in all other fish light-organ symbioses (occurring in 10 families in 5 orders), the symbionts belong t ... | 1993 | 7683390 |
| growth and luminescence of luminous bacteria promoted by agents of microbial origin. | the examination of four species of luminous bacteria photobacterium leiognathi, photobacterium phosphoreum, vibrio fischeri and vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. these agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and ... | 1993 | 8285107 |
| separation of ph, dilution, ionic strength and chemical matrix effects for biological monitoring of urines with the microtox test using nicotine, cotinine and reference urines. | the aim was to investigate the factors influencing light emission from photobacterium phosphoreum in the microtox test to interpret bioassay results for urine. four reference urines were assessed as reference materials for the bioassay. nicotine and cotinine were investigated as urinary markers for tobacco exposure. the optimum luminescence conditions were: 1.85%-3.25% nacl, 0.33-0.58 mol/l ionic strength, and ph 5.8-6.7. low ph values and high concentration of toxic trace metals were important ... | 1993 | 8475782 |
| use of the bioluminescent bacterium photobacterium phosphoreum to detect potentially biohazardous materials in water. | 1993 | 8400656 | |
| mechanism of bacterial bioluminescence: 4a,5-dihydroflavin analogs as models for luciferase hydroperoxide intermediates and the effect of substituents at the 8-position of flavin on luciferase kinetics. | bioluminescence catalyzed by bacterial luciferases was measured using fmn, iso-fmn (6-methyl-8-nor-fmn), and fmn analogs carrying the following substituents at position 8: -h, -cl, -f, sme, some, -so2me, or -ome. the first-order rate constants for the decay of light emission correlate with the one-electron oxidation potentials of the 4a,5-dihydro forms of the fmn analogs. to determine the values of these potentials, isoalloxazine (flavin) derivatives having the 4a,5-propano-4a,5-dihydro structur ... | 1993 | 8422349 |
| toxicity of produced water from crude oil terminals to photobacterium phosphoreum, chaetoceros sp., and donax faba. | 1993 | 8428121 | |
| nucleotide sequence of the luxc gene encoding fatty acid reductase of the lux operon from photobacterium leiognathi. | the nucleotide sequence of the luxc gene (embl accession no. 65156) encoding fatty acid reductase (far) of the lux operon from photobacterium leiognathi pl741 was determined and the encoded amino acid sequence deduced. the fatty acid reductase is a component of the fatty acid reductase complex. the complex is responsible for converting fatty acid to aldehyde which serves as the substrate in the luciferase-catalyzed bioluminescent reaction. the protein comprises 478 amino acid residues and has a ... | 1993 | 8447834 |
| the lumazine protein-encoding gene in photobacterium leiognathi is linked to the lux operon. | the nucleotide (nt) sequence of the lump (embl accession no. x65612) gene of photobacterium leiognathi pl741 was determined and the amino acid (aa) sequence deduced. the encoded aa sequence of lump was identified as that of the lumazine protein (lump) by homology with that of photobacterium phosphoreum (56%). this small protein has a calculated m(r) of 19,997 and comprises 186 aa residues. biochemical studies suggested that lump is the protein which, when combined with luciferase, is responsible ... | 1993 | 8472956 |