prevalence and characterization of plasmid-mediated quinolone resistance genes in aeromonas spp. isolated from south african freshwater increasing incidence of multidrug-resistant aeromonas spp., which are both fish and emerging opportunistic human pathogens, has been observed worldwide. quinolone-resistant aeromonas spp. isolates are increasingly being observed in clinical and environmental settings, and this has been attributed primarily to target gene alterations, efflux, and transferable quinolone resistance. thirty-four aeromonas spp., obtained from freshwater aquaculture systems, were screened for the presence of gyra a ...201627180024
identification of new natural cpha metallo-β-lactamases cpha4 and cpha5 in aeromonas veronii and aeromonas hydrophila isolates from municipal sewage in central italy.two new natural cpha metallo-β-lactamases, the cpha4 and cpha5 enzymes, were identified in water samples from municipal sewage in central italy. compared to cpha, the cpha4 and cpha5 enzymes showed numerous point mutations. these enzymes have a narrow spectrum of substrates focused on carbapenems only. cpha5 showed kcat values about 40-, 12-, and 97-fold higher than those observed for cpha4 versus imipenem, ertapenem, and biapenem, respectively.201525987617
molecular cloning, characterization, and expression analysis of two different types of lectins from the oriental river prawn, macrobrachium nipponense.lectins, which are widely expressed in invertebrates, play important roles in many biological processes, including protein trafficking, cell signaling, pathogen recognition, as effector molecules, and so on (wang and wang, 2013). this study identified one novel m-type lectin and one l-type lectin, designated as mnmtl1 and mnltl1, from the oriental river prawn macrobrachium nipponense. the full-length cdna of mnmtl1 was 2064 bp with a 1761 bp orf encoding a putative protein of 586 deduced amino a ...201525929240
aeromonas hydrophila and aeromonas veronii predominate among potentially pathogenic ciprofloxacin- and tetracycline-resistant aeromonas isolates from lake erie.members of the genus aeromonas are ubiquitous in nature and have increasingly been implicated in numerous diseases of humans and other animal taxa. although some species of aeromonads are human pathogens, their presence, density, and relative abundance are rarely considered in assessing water quality. the objectives of this study were to identify aeromonas species within lake erie, determine their antibiotic resistance patterns, and assess their potential pathogenicity. aeromonas strains were is ...201424242249
interaction of aeromonas strains with lactic acid bacteria via caco-2 cells.the genus aeromonas includes some species that have now been identified as human pathogens of significant medical importance. we investigated the ability of 13 selected aeromonas strains belonging to nine species isolated from clinical cases (n = 5), environmental waters (n = 5), and fish (n = 3) to adhere to and translocate caco-2 cells in the absence and presence of two lactic acid bacteria (lab), i.e., lactobacillus acidophilus and bifidobacterium breve. aeromonas isolates were also assessed ...201424242240
effects of three strains of intestinal autochthonous bacteria and their extracellular products on the immune response and disease resistance of common carp, cyprinus carpio.the study isolated three strains of intestinal autochthonous bacteria aeromonas veronii ba-1, vibrio lentus ba-2, and flavobacterium sasangense ba-3 from the intestinal tract of the common carp (cyprinus carpio). to reveal the effects of these three strains of bacteria on the innate immunity of carp, the lysozyme, complement c3, total serum protein, albumin and globulin levels, respiratory burst activity, phagocytic activity by blood leucocytes and the expression of il-1b, lysozyme-c, and tnf-α ...201424161775
protective efficacy of plga microspheres loaded with divalent dna vaccine encoding the ompa gene of aeromonas veronii and the hly gene of aeromonas hydrophila in the present study, poly (lactic-co-glycolic) acid (plga) was used as a carrier for a divalent fusion dna vaccine encoding the aeromonas veronii outer membrane protein a (ompa) and aeromonas hydrophila hemolysins (hly) protein. the recombinant pet-28a-ompa-hly was constructed by inserting the ompa gene and hly gene into a pet-28a expression vector. loading of ompa-hly antigen module on plga microspheres were accomplished by water-in-oil-in-water (w/o/w) encapsulation. the molecular weight and ...201324012571
high-yield production of a chitinase from aeromonas veronii b565 as a potential feed supplement for warm-water aquaculture.chitin, present in crustacean shells, insects, and fungi, is the second most plentiful natural organic fiber after wood. to effectively use chitin in a cost-saving and environmentally friendly way in aquaculture, crustacean shells (e.g., shrimp-shell meal) are supplemented into aquafeed after degradation by chemical methods. herein, we describe a chitinase from aeromonas veronii b565, designated chib565, which potently degrades shrimp-shell chitin and resists proteolysis. we isolated recombinant ...201423775269
infections following the application of leeches: two case reports and review of the literature. 201223098279
identity and virulence properties of aeromonas isolates from diseased fish, healthy controls and water environment in investigate the species distribution in aeromonas isolates from diseased fish, healthy controls and water environment in china; to evaluate the frequency of the aerolysin (aer), cytotonic enterotoxin (alt), cytotoxic enterotoxin (act), temperature-sensitive protease (eprcai) and serine protease (ahp) genes in aeromonas isolates; and to determine the potential pathogenicity of these isolates.201222725694
recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda.the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ...201222564558
detection of aerolysin gene in aeromonas hydrophila isolated from fish and pond water.aerolysin is a hemolytic toxin encoded by aerolysin gene (1482 bp) that plays a key role in the pathogenesis of aeromonas hydrophila infection in fish. new speciesspecific primers were designed to amplify 326 bp conserved region of aerolysin gene for a. hydrophila. twenty-five isolates of a. hydrophila recovered from fish and pond water were studied for detection of aerolysin gene. aerolysin gene was detected in 85% of the isolates during the study. the designed primers were highly specific and ...200823100746
draft genome sequences of amoeba-resistant aeromonas spp. isolated from aquatic environments.amoeba-resistant aeromonas veronii arb3 and aeromonas media arb13 and arb20, which may be important intracellular pathogens of eukaryotic hosts, were isolated from pond and river waters. the draft genome sequences indicate that the strains harbor multiple protein secretion systems and toxins that induce disruption of the actin cytoskeleton.201425359918
broad diversity of conjugative plasmids in integron-carrying bacteria from wastewater this study we assessed the occurrence, diversity and conjugative potential of plasmids in integron-carrying aeromonas and enterobacteriaceae from wastewaters. sixty-six strains were included as donors in mating assays using rifampicin-resistant escherichia coli and pseudomonas putida recipient strains. the diversity of plasmids from donors and transconjugants (resistant to tetracycline or streptomycin) was evaluated by restriction analysis and replicon typing targeting 19 incompatibility grou ...201222409355
aeromonas sobria necrotizing fasciitis and sepsis in an immunocompromised patient: a case report and review of the literature.aeromonas veronii biovar sobria is a rare cause of bacteremia, with several studies indicating that this isolate may be of particular clinical significance since it is enterotoxin producing. a wide spectrum of infections has been associated with aeromonas species in developing countries that include gastroenteritis, wound infections, septicemia and lung infections. this infection, caused by aeromonas species, is usually more severe in immunocompromised than immunocompetent individuals. we here d ...201425245365
carbapenemase-producing aeromonas veronii disseminated in the environment of an equine specialty hospital.the emergence of bacterial resistance to carbapenem antibiotics is an urgent public health threat. carbapenem drugs are a last resort treatment option for life-threatening infections. the frequent use of broad-spectrum antibiotics to treat hospitalized patients provides significant selection pressure favoring the emergence and dissemination of resistant organisms, including carbapenem-resistant enterobacteriaceae (cre). cres have been reported in animal populations, but only rarely in horses. ou ...201728346803
effects of dietary administration of shewanella xiamenensis a-1, aeromonas veronii a-7, and bacillus subtilis, single or combined, on the grass carp (ctenopharyngodon idella) intestinal microbiota.gut microbiota of grass carp plays an important role in host. however, detailed information regarding the changes of microbiota after probiotics administration in relation to the gastrointestinal microbiota is absent. in the present study, dietary administration of putative probiotics shewanella xiamenensis a-1, aeromonas veronii a-7, and bacillus subtilisstrains was conducted in grass carp to investigate if there is a discernible alteration in intestinal microbiota and whether the alteration is ...201728321827
collection of mullet fish (mugil cephalus) from west coast of india: evaluation of its quality with relation to food safety.the present study focuses on bacterial and selected heavy metal contaminations of economically important mullet fish (mugil cephalus) collected from west coast of gujarat, india. molecular identification using 16s rrna sequencing revealed the presence of aeromonas veronii, a. mollusorum, a. cavae, a. bivalvum, and v.alginolyticus in the gill and the intestine along with some other non-pathogenic bacteria. pathogenicity of different aeromonas species was confirmed by hemolysin assay. apart from p ...201728290091
neutrophils exert protection in early aeromonas veronii infections through the clearance of both bacteria and dying macrophages.aeromonas veronii is a gram-negative opportunistic pathogen capable of infecting both fish and mammals. left untreated, natural infection in fish can prove fatal and result in irreparable damage to the aquaculture industry. neutrophils are essential innate effector cells that play critical roles in pathogen defense. our aim was to investigate the immunological roles of teleost neutrophils during infection with a. veronii. we began by examining the functional defenses of neutrophils in vitro, whe ...201728167251
diversity of culturable bacterial communities in the intestinal tracts of goldfish (carassius auratus) and their ability to produce n-acyl homoserine lactone.intestinal bacteria isolated from goldfish (carassius auratus) were identified based on 16 ribosomal rna (rrna) gene sequences and screened for their ability to produce n-acyl homoserine lactone (ahl), an autoinducer of the quorum sensing (qs) system. the 230 aerobes/facultative anaerobes that were isolated comprised members of the genera aeromonas (184 isolates), citrobacter (11), enterobacter (2), shewanella (28), vagococcus (1), and vibrio (4). among these genera, the two most abundant specie ...201728124783
dietary zinc promotes immuno-biochemical plasticity and protects fish against multiple stresses.the abiotic and biotic stress is an episode that effect on regulatory, neuro-endocrine and immune systems of animals including fish. the stress creates stimulatory and suppressive of immune system resulting in increases the incidence of infection. in view of these points, we have conducted an experiment to mitigate the stress through a nutritional approach through zinc (zn) supplementation in pangasius hypophthalmus (initial weight-3.65 ± 0.75 g). three isocaloric and isonitrogenous diets with g ...201728108338
genome sequencing and annotation of aeromonas veronii strain ae52, a multidrug-resistant isolate from septicaemic gold fish (carassius auratus) in sri we report the draft genome sequence and annotation of a. veronii strain ae52 isolated from the kidney of a morbund, septicaemic gold fish (carassius auratus) in sri lanka. this clinical isolate showed resistance to multiple antimicrobials; amoxicillin, neomycin, trimethoprim-sulphonamide, chloramphenicol, tetracycline, enrofloxacin, erythromycin and nitrofurantoin. the size of the draft genome is 4.56 mbp with 58.66% of g + c content consisting 4328 coding sequences. it harbors a repertoire ...201727942459
host matters: medicinal leech digestive-tract symbionts and their pathogenic potential.digestive-tract microbiota exert tremendous influence over host health. host-symbiont model systems are studied to investigate how symbioses are initiated and maintained, as well as to identify host processes affected by resident microbiota. the medicinal leech, hirudo verbana, is an excellent model to address such questions owing to a microbiome that is consistently dominated by two species, aeromonas veronii and mucinivorans hirudinis, both of which are cultivable and have sequenced genomes. t ...201627790190
antibiotic resistance and repetitive-element pcr fingerprinting in aeromonas veronii isolates.this study evaluated antibiotic resistance and the related genes in total 47 aeromonas veronii isolates from pet fish, eel (anguilla japonica) and koi (cyprinus carpio) in korea. in comparison with the antibiotic susceptibilities of isolates from eel and koi, those of pet fish were more resistant to ceftiofur, aminoglycosides, tetracycline and nitrofurantoin. and isolates from pet fish showed high prevalences of class 1 integron, quinolones and tetracycline resistance determinants than those fro ...201627690177
genetic selection of peptide aptamers that interact and inhibit both small protein b and alternative ribosome-rescue factor a of aeromonas veronii c4.aeromonas veronii is a pathogenic gram-negative bacterium, which infects a variety of animals and results in mass mortality. the stalled-ribosome rescues are reported to ensure viability and virulence under stress conditions, of which primarily include trans-translation and alternative ribosome-rescue factor a (arfa) in a. veronii. for identification of specific peptides that interact and inhibit the stalled-ribosome rescues, peptide aptamer library (ptrg-sn-peptides) was constructed using ptrg ...201627588015
draft genome sequence of aeromonas sp. strain eerv15.we report here the draft genome sequence of aeromonas sp. strain eerv15 isolated from sand filter. the organism most closely related to aeromonas sp. eerv15 is aeromonas veronii b565, with an average 83% amino acid sequence similarity of putatively encoded protein open reading frames.201627540061
multidrug-resistant aeromonas veronii recovered from channel catfish (ictalurus punctatus) in china: prevalence and mechanisms of fluoroquinolone emphasize the importance of the appropriate use of antibiotics in aquaculture systems, the prevalence of resistance to 25 antimicrobials was investigated in 42 aeromonas veronii strains isolated from farm-raised channel catfish in china in 2006-2012. all experiments were based on minimal inhibitory concentrations (mics), and susceptibility was assessed according to the clinical and laboratory standards institute. some isolates displayed antibiotic resistance to the latest-generation fluoroqui ...201627483342
[correlation between maldi-tof vitek-mstm system and conventional identification methods of gastrointestinal infection causing bacteria].rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. matrix-assisted laser desorption/ionization time of flight (maldi-tof) mass spectrometry has shown to be effective and fast for the identification of microorganisms. the objective of this study was to evaluate the correlation between vitek-mstm and conventional methods for bacterial identification causing gastrointestinal infection.201627454012
establishment and characterization of a skin epidermal cell line from mud loach, misgurnus anguillicaudatus, (mase) and its interaction with three bacterial pathogens.a continuous skin epidermal cell line from mud loach (misgurnus anguillicaudatus) (mase cell line) was established with its application in bacteria infection demonstrated in this study. primary mase cell culture was initiated at 26 °c in dulbecco's modified eagle medium/f12 medium (1:1; ph7.2) supplemented with 20% fetal bovine serum (fbs). the primary mase cells in spindle morphology proliferated into a confluent monolayer within 2 weeks, and were continuously subcultured even in 10% fbs- dmem/ ...201627288257
complete genome sequence of the fish pathogen aeromonas veronii th0426 with potential application in biosynthesis of pullulanase and chitinase.aeromonas veronii th0426 is a pathogen of the farmed yellow catfish pelteobagrus fulvidraco but shows high-level expression of pullulanase and chitinase. here, we present its genome sequence, which is the first reported complete genome of fish pathogen in a. veronii to date. strain th0426 harbors a single circular 4,923,009bp chromosome with a gc content of 58.25%. there are 4525 genes identified on its genome, including 4244 protein-coding genes, 32 rrna genes, 120 trna genes, a noncoding rna a ...201627080448
characterization of virulence properties of aeromonas veronii isolated from diseased gibel carp (carassius gibelio).aeromonas veronii is a kind of opportunistic pathogen to fish and humans, significantly impending aquaculture production. recently, we isolated two a. veronii strains, named gyc1 and gyc2, from diseased gibel carp (carassius gibelio) in china. based on gyrb (dna gyrase b subunit) genes of gyc1 and gyc2, the constructed phylogenetic tree showed that the two strains were clustered with a. veronii. sixteen virulence genes related to the pathogenicity of aeromonas spp. were subjected to pcr assay. t ...201627043558
medicinal leech therapy and aeromonas spp. infection.while the use of medicinal leech therapy (mlt) in reconstructive and orthopaedic surgery is widely described, post-operative complications related to leeches remain a major concern. aeromonas spp. strains are involved in the majority of reported cases. as surgical success rate is directly impacted, an adapted antibiotic prophylaxis should be instituted in order to minimize these complications. we assessed pharmaceutical process, microbiological control and related infections in order to provide ...201627039338
n-acyl homoserine lactone-mediated quorum sensing in aeromonas veronii biovar sobria strain 159: identification of luxri homologs. 201626909339
a tale of transmission: aeromonas veronii activity within leech-exuded mucus.transmission, critical to the establishment and persistence of host-associated microbiotas, also exposes symbionts to new environmental conditions. with horizontal transmission, these different conditions represent major lifestyle shifts. yet genome-wide analyses of how microbes adjust their transcriptomes toward these dramatic shifts remain understudied. here, we provide a comprehensive and comparative analysis of the global transcriptional profiles of a symbiont as it shifts between lifestyles ...201626896136
reciprocal immune benefit based on complementary production of antibiotics by the leech hirudo verbana and its gut symbiont aeromonas veronii.the medicinal leech has established a long-term mutualistic association with aeromonas veronii, a versatile bacterium which can also display free-living waterborne and fish- or human-pathogenic lifestyles. here, we investigated the role of antibiotics in the dynamics of interaction between the leech and its gut symbiont aeromonas. by combining biochemical and molecular approaches, we isolated and identified for the first time the antimicrobial peptides (amps) produced by the leech digestive trac ...201526635240
identification and virulence properties of aeromonas veronii bv. sobria isolates causing an ulcerative syndrome of loach misgurnus anguillicaudatus. 201626525163
identification of population bottlenecks and colonization factors during assembly of bacterial communities within the zebrafish intestine.the zebrafish, danio rerio, is a powerful model for studying bacterial colonization of the vertebrate intestine, but the genes required by commensal bacteria to colonize the zebrafish gut have not yet been interrogated on a genome-wide level. here we apply a high-throughput transposon mutagenesis screen to aeromonas veronii hm21 and vibrio sp. strain zwu0020 during their colonization of the zebrafish intestine alone and in competition with each other, as well as in different colonization orders. ...201526507229
biofilm formation on the surface of polylactide during its biodegradation in different environments.the research was aimed at determining the abundance and viability of biofilm formed on the surface of polylactide (pla) during its biodegradation in different environments. it was also aimed at isolating biofilm forming bacteria, determining their hydrolytic activity and taxonomic status. the first step was to evaluate pla biodegradability in lake water, compost and soil, using oxitop control. the next step was to assess the ability of isolated bacteria to form biofilm in the investigated enviro ...201526433346
improvement of methyl orange dye biotreatment by a novel isolated strain, aeromonas veronii gri, by spb1 biosurfactant addition.aeromonas veronii gri (kf964486), isolated from acclimated textile effluent after selective enrichment on azo dye, was assessed for methyl orange biodegradation potency. results suggested the potential of this bacterium for use in effective treatment of azo-dye-contaminated wastewaters under static conditions at neutral and alkaline ph value, characteristic of typical textile effluents. the strain could tolerate higher doses of dyes as it was able to decolorize up to 1000 mg/l. when used as micr ...201626396008
extensive community-acquired pneumonia with hemophagocytic syndrome caused by aeromonas veronii in an immunocompetent patient. 201526318349
detection and characterization of antimicrobial resistance and putative virulence genes in aeromonas veronii biovar sobria isolated from gilthead sea bream (sparus aurata l.).the purposes of this study were to investigate the antimicrobial resistance of aeromonas veronii biovar sobria isolated from gilthead sea bream and to characterize the virulence-implicated genes. fish samples (n=365) were collected from wholesale and retail markets in aljouf, saudi arabia between 2013 and 2014. a total of 45 a. veronii biovar sobria isolates (12.3%) from those samples were tested for resistance to a range of antimicrobial agents. all strains exhibited 100% resistances to nalidix ...201526218714
occurrence of glucocorticoids discharged from a sewage treatment plant in japan and the effects of clobetasol propionate exposure on the immune responses of common carp (cyprinus carpio) to bacterial infection.the present study evaluated the environmental risks to common carp (cyprinus carpio) posed by glucocorticoids present in sewage treatment plant (stp) effluent. to gather information on the seasonal variations in glucocorticoid concentration, the authors sampled the effluent of a japanese stp every other week for 12 mo. six of 9 selected glucocorticoids were detected in the effluent, with clobetasol propionate and betamethasone 17-valerate detected at the highest concentrations and frequencies. t ...201626126539
a novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural, we report on the construction of a metagenomic library from a chitin-amended disease-suppressive agricultural soil and its screening for genes that encode novel chitinolytic enzymes. the library, constructed in fosmids in an escherichia coli host, comprised 145,000 clones containing inserts of sizes of 21 to 40 kb, yielding a total of approximately 5.8 gb of cloned soil dna. using genetic screenings by repeated pcr cycles aimed to detect gene sequences of the bacterial chitinase a-class (h ...201526040993
a new alkaline lipase obtained from the metagenome of marine sponge ircinia sp.microorganisms associated with marine sponges are potential resources for marine enzymes. in this study, culture-independent metagenomic approach was used to isolate lipases from the complex microbiome of the sponge ircinia sp. obtained from the south china sea. a metagenomic library was constructed, containing 6568 clones, and functional screening on 1 % tributyrin agar resulted in the identification of a positive lipase clone (35f4). following sequence analysis 35f4 clone was found to contain ...201525921581
multi-parametric analysis of phagocyte antimicrobial responses using imaging flow cytometry.we feature a multi-parametric approach based on an imaging flow cytometry platform for examining phagocyte antimicrobial responses against the gram-negative bacterium aeromonas veronii. this pathogen is known to induce strong inflammatory responses across a broad range of animal species, including humans. we examined the contribution of a. veronii to the induction of early phagocyte inflammatory processes in raw 264.7 murine macrophages in vitro. we found that a. veronii, both in live or heat-ki ...201525862969
impact of hydrocarbons, pcbs and heavy metals on bacterial communities in lerma river, salamanca, mexico: investigation of hydrocarbon degradation potential.freshwater contamination usually comes from runoff water or direct wastewater discharges to the environment. this paper presents a case study which reveals the impact of these types of contamination on the sediment bacterial population. a small stretch of lerma river basin, heavily impacted by industrial activities and urban wastewater release, was studied. due to industrial inputs, the sediments are characterized by strong hydrocarbon concentrations, ranging from 2 935 to 28 430μg·kg(-1) of tot ...201525828406
characterization of shed medicinal leech mucus reveals a diverse microbiota.microbial transmission through mucosal-mediated mechanisms is widespread throughout the animal kingdom. one example of this occurs with hirudo verbana, the medicinal leech, where host attraction to shed conspecific mucus facilitates horizontal transmission of a predominant gut symbiont, the gammaproteobacterium aeromonas veronii. however, whether this mucus may harbor other bacteria has not been examined. here, we characterize the microbiota of shed leech mucus through illumina deep sequencing o ...201425620963
humoral immune response and tlr9 gene expression in pacific red snapper (lutjanus peru) experimentally exposed to aeromonas veronii.aquaculture production of pacific red snapper lutjanus peru is growing rapidly in mexico, especially in gulf of california. as it is a relatively new aquaculture species there are few reports evaluating its immune response to pathogens. the gram-negative bacteria aeromonas veronii is a heterogeneous organism that causes the disease known as motile aeromonad septicemia, which is responsible for serious economic loss in seabream culture due to bacterial infections. for the purpose of this study, j ...201525462554
aeromonas stool isolates from individuals with or without diarrhea in southern taiwan: predominance of aeromonas veronii.although aeromonads are important pathogens causing invasive infections in southern taiwan, aeromonas-associated intestinal infections have been rarely mentioned.201525440979
screening of microorganisms from deep-sea mud for antarctic krill (euphausia superba) fermentation and evaluation of the bioactive compounds.twelve kinds of strains were isolated from deep-sea mud which can use antarctic krill powder as the sole carbon/nitrogen source. these strains were identified by 16s rdna sequence analysis and grouped into eight different genera, including bacillus, shewanella, psychrobacter, klebsiella, macrococcus, aeromonas, acinetobacter, and saccharomyces. after fermentation of antarctic krill powder using these strains, bioactive compounds including total phenolics, free amino acids, and enzyme activities ...201525416479
microbial succession of grass carp (ctenopharyngodon idellus) filets during storage at 4°c and its contribution to biogenic amines' formation.investigation on the microbial succession of grass carp filets during storage at 4°c was carried out. for identification, 16s rrna genes of the isolated pure strains were sequenced and analyzed. acinetobacter was dominant in fresh grass carp. species from the genera brevundimonas, empedobacter, pseudomonas, microbacterium, flavobacterium, moraxella, shewanella and soonwooa were also detected at the initial day. the communities were dominated by aeromonas and acinetobacter after 6days. aeromonas ...201425194259
hitchhiking of host biology by beneficial symbionts enhances transmission.transmission plays a key role in the evolution of symbiosis. mixed mode transmission combines horizontal and vertical mechanisms for symbiont acquisition. however, features that enable mixed transmission are poorly understood. here, we determine the mechanistic basis for the recruitment of the beneficial bacterium, aeromonas veronii by the leech, hirudo verbana. we demonstrate that host mucosal secretions complement imperfect symbiont vertical transmission. first, we show that the a. veronii pop ...201425059557
diaryl-substituted azolylthioacetamides: inhibitor discovery of new delhi metallo-β-lactamase-1 (ndm-1).the emergence and spread of antibiotic-resistant pathogens is a global public health problem. metallo-β-lactamases (mβls) such as new delhi mβl-1 (ndm-1) are principle contributors to the emergence of resistance because of their ability to hydrolyze almost all known β-lactam antibiotics including penicillins, cephalosporins, and carbapenems. a clinical inhibitor of mbls has not yet been found. in this study we developed eighteen new diaryl-substituted azolylthioacetamides and found all of them t ...201425048031
metagenomic analysis of the medicinal leech gut microbiota.there are trillions of microbes found throughout the human body and they exceed the number of eukaryotic cells by 10-fold. metagenomic studies have revealed that the majority of these microbes are found within the gut, playing an important role in the host's digestion and nutrition. the complexity of the animal digestive tract, unculturable microbes, and the lack of genetic tools for most culturable microbes make it challenging to explore the nature of these microbial interactions within this ni ...201424860552
structural studies of the lipopolysaccharide from the fish pathogen aeromonas veronii strain bs19, serotype o16.chemical analyses, mass spectrometry, and nmr spectroscopy were applied to study the structure of the lipopolysaccharide (lps) isolated from aeromonas veronii strain bs19, serotype o16. esi-ms revealed that the most abundant lps glycoforms have tetra-acylated or hexa-acylated lipid a species, consisting of a bisphosphorylated glcn disaccharide with an aran residue as a non-stoichiometric substituent, and a core oligosaccharide composed of hep₅hex₃hexn₁kdo₁p₁. sugar and methylation analysis toget ...201424608968
characterization of the digestive tract microbiota of hirudo orientalis (medicinal leech) and antibiotic resistance profile.there are at least three distinct european leech species used medicinally: hirudo medicinalis, h. orientalis, and h. verbana. infection caused by leech microbiota is the most widely reported complication. few studies have reported the culturable and unculturable bacteria and examined the antibiotic resistances in h. orientalis.201424572887
contribution of hot spring bacterial consortium in cadmium and lead bioremediation through quadratic programming the present investigation, a number of experiments have been conducted to isolate microbial strains from taptapani hot spring odisha, india for bioremediation of cadmium and lead. the strains stenotrophomonas maltophilia (ss1), aeromonas veronii (ss2) and bacillus barbaricus (ss3) have shown better adaptation to metal tolerance test, with different concentrations of cadmium and lead and hence have been selected for further studies of metal microbial interaction and optimization. the results o ...201424333714
draft genome sequence of aeromonas veronii hm21, a symbiotic isolate from the medicinal leech digestive tract.aeromonas veronii strain hm21 was isolated from the digestive tract of the medicinal leech hirudo verbana and has been used to identify genes that are important for host colonization. this species is also a symbiont in the gut of zebrafish and is a pathogen of mammals and fish. we present here a 4.68-mbp draft genome sequence for hm21.201324092791
identification of a bacteriovorax sp. isolate as a potential biocontrol bacterium against snakehead fish-pathogenic aeromonas veronii. 201423745946
suicide plasmid-dependent is1-element untargeted integration into aeromonas veronii bv. sobria generates brown pigment-producing and spontaneous pelleting mutant.foodborne gram-negative pathogens belonging to the genus aeromonas are variable in harboring insertion sequence (is) elements that play an important role in the generation of dysfunctional relatives of known genes. using suicide plasmids carrying an is1-element, untargeted integration is a common problem during experimental trials to generate specific mutations by homologous recombination. in this work, different strains of aeromonas veronii bv. sobria (aeg1 and atcc 9071t), a. hydrophila atcc 1 ...201323436029
tissue distribution, expression, and antimicrobial activity of anas platyrhynchos avian β-defensin this study, a novel avian β-defensin (avbd) was isolated from the chaohu duck. the complete nucleotide sequence of the gene contained a 204-bp open reading frame that encoded 67 amino acids (aa), including a signal peptide of 20 aa, a propiece of 5 aa, and a mature peptide of 42 aa. the homology, characterization, and comparison of this gene with avbd from other avian species confirmed that it was duck avbd6. also, the preproprotein of avbd6 from chicken, goose, and duck was highly conserved ...201323243235
resistance characterization, virulence factors, and eric-pcr fingerprinting of aeromonas veronii strains isolated from diseased trionyx sinensis.aeromonas veronii (av) is an important pathogen causing severe diseases in aquaculture. fifteen a. veronii strains isolated from diseased trionyx sinensis from four aquafarms was characterized by enterobacterial repetitive intergenic consensus-polymerase chain reaction (eric-pcr), antibiotic susceptibility testing, and identification of virulence factors. a. veronii strains were grouped into nine eric types with discriminatory ability (d) of 91.43 based on 90% similarity. the virulence genes wer ...201223134287
a severe case of aeromonas veronii biovar sobria travellers' diarrhoea characterized by vibrio parahaemolyticus co-isolation.we report a severe case of travellers' diarrhoea in a patient returning from ecuador to italy with the concomitant presence of aeromonas veronii biovar sobria and vibrio parahaemolyticus in their faeces. based on diagnostic results, epidemiological information and the clinical outcome, we conclude that the real aetiological agent was a. veronii biovar sobria, while v. parahaemolyticus was only transient in the intestine of the patient.201323002063
effects of marine silages enriched with lactobacillus sakei 5-4 on haemato-immunological and growth response in pacific red snapper (lutjanus peru) exposed to aeromonas veronii.combined effects of marine silages enriched with lactobacillus sakei 5-4 were evaluated on growth performance, immune activity and disease resistance of pacific red snapper (lutjanus peru) against aeromonas veronii infection. the experimental fish were divided into three groups which were fed with each one of the following diets: silage-probiotic-free diet (control, c group), pacific creole-fish silage diet supplemented with live l. sakei (10(6) cfu g(-1)) (fslact group) and humboldt squid silag ...201222940556
characterization and virulence potential of phenotypically diverse aeromonas veronii isolates recovered from moribund freshwater ornamental fishes of kerala, the present study, we investigated the involvement of aeromonas spp. in eliciting disease outbreaks in freshwater ornamental fishes across the state of kerala, india. we investigated three incidences of disease, in which the moribund fishes exhibited clinical signs such as haemorrhagic septicemia (in gouramy, trichogaster sp.), dropsy (in oscar, astronotus ocellatus) and tail rot/fin rot (in gold fish, carassius carassius). pure cultures (n = 20 from each fish; 60 in total) of aeromonas spp. ...201322903451
bacterial symbioses of the medicinal leech hirudo verbana.gastrointestinal microbiomes play important roles in the health and nutrition of animals and humans. the medicinal leech, hirudo verbana, serves as a powerful model for the study of microbial symbioses of the gut, due to its naturally limited microbiome compared with other popular models, the ability to cultivate the most abundant microbes, and genetically manipulate one of them, aeromonas veronii. this review covers the relevance and application of leeches in modern medicine as well as recent d ...201722572874
structural analysis of the o-specific polysaccharide from the lipopolysaccharide of aeromonas veronii bv. sobria strain k49.the o-specific polysaccharide obtained by mild-acid degradation of the lipopolysaccharide from aeromonas veronii bv. sobria strain k49 was studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy. the sequence of the sugar residues was determined using (1)h,(1)h noesy and (1)h,(13)c hmbc experiments. the o-specific polysaccharide was found to be a high molecular mass polysaccharide composed of repeating units of the structure: →2)-β-d-quip3nac-(1→3)-α-l-rhap-(1→3)-α-l ...201222483338
bundle-forming pilus locus of aeromonas veronii bv. sobria.little is known about the colonization mechanisms of aeromonas spp. previous work has suggested that the type iv bundle-forming pilus (bfp) is an aeromonad intestinal colonization factor. this study provides the first genetic characterization of this structure. to define the role of bfp in aeromonas veronii bv. sobria adherence, a 22-kb locus encoding the bundle-forming pilus was isolated; this contained 17 pilus-related genes similar to the mannose-sensitive hemagglutinin (msha) of vibrio chole ...201222311923
characterization of pathogenic aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (leiocassis longirostris günther).273 bacterial strains were isolated from 20 chinese longsnout catfish samples. the biochemical characteristics of all strains conformed to the species description of aeromonas veronii bv. veronii on the basis of vitek gni+ card. furthermore, 16s rdna, gyrb and rpod sequences of the representative strain py50 were sequenced and showed high similarity with a. veronii bv. veronii in genbank. antibiotic-resistance of the representative strain py50 was assessed by the kirby-bauer disk diffusion metho ...201224031843
n-acyl homoserine lactone lactonase, aiia, inactivation of quorum-sensing agonists produced by chlamydomonas reinhardtii (chlorophyta) and characterization of aiia transgenic algae(1).eukaryotes such as plants and the unicellular green alga chlamydomonas reinhardtii p. a. dang. produce and secrete compounds that mimic n-acyl homoserine lactone (ahl) bacterial quorum-sensing (qs) signals and alter qs-regulated gene expression in the associated bacteria. here, we show that the set of c. reinhardtii signal-mimic compounds that activate the cepr ahl receptor of burkholderia cepacia are susceptible to inactivation by aiia, an ahl lactonase enzyme of bacillus. inactivation of these ...201127020200
distribution of alginate genes in bacterial isolates from corroded metal surfaces.the distribution of alginate genes encoding biosynthesis of alginate was examined for bacterial isolates associated with corrosive biofilms recovered from source water, cooling lines, and reactor surfaces of a nuclear power plant. a total of 120 diverse gram-positive and -negative isolates were obtained. using dna:dna hybridization, 11 isolates were shown to contain sequences homologous to structural (algd, algg, alg-76) and/or regulatory (albb) alginate biosynthetic genes derived from an algina ...199424190336
production of polyhydroxyalkanoates (pha) using sludge from different wastewater treatment processes and the potential for medical and pharmaceutical this study, seven strains of bacteria with polyhydroxyalkanoates (pha)-producing ability (i.e., bacillus cereus, pseudomonas putida, bacillus pumilus, pseudomona-huttiensis, yersinia-frederiksenii, aeromonas ichthiosmia and sphingopyxis terrae) were isolated from various waste treatment plants in hong kong. simultaneous wastewater treatment and pha accumulation were successfully achieved in the bioreactors using isolated bacteria from different sludges. at the organic loading less than 13000 ...201728387154
chromosome-mediated mcr-3 variants in aeromonas veronii from chicken meat.two adjacent colistin resistance gene variants, termed mcr-3.2 and mcr-3-like, were identified in the chromosome of an aeromonas veronii isolated from retail chicken meat. the variants showed 95.20% and 84.19% nucleotide sequence identity, respectively, to mcr-3 from porcine escherichia coli functional cloning indicated that only mcr-3.2 conferred polymyxin resistance in both e. coli and aeromonas salmonicida the mcr-3.2-mcr-3-like segment was also observed in other aeromonas species, including ...201728848017
enhancement of the production of l-glutaminase, an anticancer enzyme, from aeromonas veronii by adaptive and induced mutation techniques.microbial anti-cancer enzymes have been proven to be effective and economical agents for cancer treatment. aeromonas veronii has been identified as a microorganism with the potential to produce l-glutaminase, an anticancer agent effective against acute lymphocytic leukaemia. in this study, a selective medium of aeromonas veronii was used to culture the microorganism. strain improvement was done by adaptive and induced mutational techniques. a selective minimal agar media was incorporated for the ...201728813436
the prevalence of antibiotic-resistant bacteria (arb) in waters of the lower ballona creek watershed, los angeles county, california.screening for the prevalence of antibiotic-resistant bacteria (arb) was done at the ballona creek and wetlands, an urban-impacted wetland system in los angeles, california. the goals were (1) to assess the overall prevalence of arb, and (2) compare differences in arb abundance and the types of antibiotic resistance (ar) among the following sample types: lagoon water from del rey lagoon, urban runoff from ballona creek, and water from the ballona wetlands (tidal water flooding in from the adjacen ...201728488226
effects of quorum quenching by ahl lactonase on ahls, protease, motility and proteome patterns in aeromonas veronii spoilage by some bacteria is reported to be regulated by quorum sensing (qs). in this study, a quorum quenching approach was used to investigate the qs regulated phenotypes (growth, protease and motility) and proteins expression in of aeromonas veronii lp-11, which is a specific spoilage organism of sturgeon. ahl lactonase aiiaai96 from bacillus quenched the qs system, probably by enzymatically inactivating the ahls produced by a. veronii lp-11. after aiiaai96 treatment, the protease and mo ...201728482205
first isolation and characterization of the pathogenic aeromonas veronii bv. veronii associated with ulcerative syndrome in the indigenous pelophylax ridibundus of al-ahsaa, saudi arabia.virulent microbial pathogen infections are the main cause for amphibian decline worldwide. in the present study, a bacterial strain named rdl-2, which was isolated from the skin of infected pelophylax ridibundus larvae, was cultured and then phenotypically and biochemically characterized using scanning electron microscopic observations and the api20e strip and biolog gen iii microplate system. the 16s rrna gene sequence of this strain was also obtained and used in strain identification and phylo ...201729038059
speciation, clinical profile & antibiotic resistance in aeromonas species isolated from cholera-like illnesses in a tertiary care hospital in north india.aeromonas species have been reported to cause various illnesses in humans such as wound infections, septicaemia, peritonitis and pneumonia. their role in causation of cholera-like illness is also being increasingly recognized. this retrospective study was done to know the presence of aeromonas as a cause of acute diarrhoea in a tertiary care hospital and to find the common species of aeromonas causing diarrhoea and their antibiotic susceptibility patterns.201729205196
prevalence and characterisation of quinolone resistance genes in aeromonas spp. isolated from pet turtles in south korea.the aim of this study was to characterise aeromonas spp. isolated from popular species of pet turtle to assess the potential risk of pet turtles as a source of target gene alterations in the quinolone resistance-determining region (qrdr) and transferable plasmid-mediated quinolone resistance (pmqr) genes.201728743647
[investigation of colistin resistance gene mcr in gut bacteria from patients with acute diarrheal].objective: to investigate the presence of colistin resistance gene mcr in gut bacteria from patients with acute diarrhea. methods: fresh stool samples were collected from 150 outpatients with acute diarrhea in the intestinal clinic of henan provincial people's hospital, and were directly detected for the presence of mcr by pcr after enrichment in the broth.the mcr-producing bacteria were further isolated and identified by maldi-tof ms (bruker biotyper). antimicrobial susceptibility of these isol ...201729325326
characterization of multiple antibiotic resistance of culturable microorganisms and metagenomic analysis of total microbial diversity of marine fish sold in retail shops in mumbai, india.marine fish species were analyzed for culturable and total metagenomic microbial diversity, antibiotic resistance (ar) pattern, and horizontal gene transfer in culturable microorganisms. we observed a high ar microbial load of 3 to 4 log cfu g-1. many fish pathogens like providencia, staphylococcus, klebsiella pneumoniae, enterobacter, vagococcus, and aeromonas veronii were isolated. photobacterium and vibrio were two major fish and human pathogens which were identified in the fish metagenome. o ...201729243150
functional characterization of probiotic potential of novel pigmented bacterial strains for aquaculture applications.the bioprospecting proficient of novel pigmented probiotic strains with respect to aquaculture industry was unexplored hitherto. in this study, we investigated the probiotic potential of novel pigmented bacterial strains isolated from the indigenous soil sediments in their vicinal habitats, which were screened for their antimicrobial activity against aquatic pathogens using agar well diffusion assay. the strains namely exiguobacterium acetylicum (s01), aeromonas veronii (v03), and chryseobacteri ...201729181803
oral immunization with recombinant lactobacillus casei expressing ompai confers protection against aeromonas veronii challenge in common carp, cyprinus carpio.aeromonas veronii is a gram-negative pathogen capable of infecting both fish and mammals, including humans, and natural infection in fish results in irreparable damage to the aquaculture industry. lactic acid bacteria (lab) have a number of properties that make them attractive candidates as delivery vehicles for presentation to the mucosa sites of compounds with pharmaceutical interest, in particular vaccines. in this study, we generated two recombinant lactobacillus casei (surface-displayed or ...201829155272
isolation and characterization of a novel, t7-like phage against aeromonas veronii.a virulent aeromonas veronii biovar sobria and the corresponding novel, lytic bacteriophage (vtccbpa5) were isolated from village pond water. the phage was found to belong to family podoviridae. pcr analysis of major capsid protein gene confirmed its classification to t7-like genus. the protein profiling by sds-page indicated the major structural protein to be ~ 45 kda. the phage (vtccbpa5) is host specific and is stable over a range of ph (6-10) and temperatures (4-45 °c). on the basis of restr ...201729116575
targeting inhibition of smpb by peptide aptamer attenuates the virulence to protect zebrafish against aeromonas veronii infection.aeromonas veronii is an important pathogen of aquatic animals, wherein small protein b (smpb) is required for pathogenesis by functioning as both a component in stalled-ribosome rescue and a transcription factor in upregulation of virulence gene bvgs expression. here a specific peptide aptamer pa-1 was selected from peptide aptamer library by bacterial two-hybrid system employing pbt-smpb as bait. the binding affinity between smpb and pa-1 was evaluated using enzyme-linked immunosorbent assay. t ...201728955325
microbial colonization is required for normal neurobehavioral development in zebrafish.changes in resident microbiota may have wide-ranging effects on human health. we investigated whether early life microbial disruption alters neurodevelopment and behavior in larval zebrafish. conventionally colonized, axenic, and axenic larvae colonized at 1 day post fertilization (dpf) were evaluated using a standard locomotor assay. at 10 dpf, axenic zebrafish exhibited hyperactivity compared to conventionalized and conventionally colonized controls. impairment of host colonization using antib ...201728894128
improving extracellular production of serratia marcescens lytic polysaccharide monooxygenase cbp21 and aeromonas veronii b565 chitinase chi92 in escherichia coli and their synergism.lytic polysaccharide monooxygenases (lpmos) can oxidize recalcitrant polysaccharides and boost the conversion of the second most abundant polysaccharide chitin by chitinase. in this study, we aimed to achieve the efficient extracellular production of serratia marcescens lpmo cbp21 and aeromonas veronii b565 chitinase chi92 by escherichia coli. twelve signal peptides reported with high secretion efficiency were screened to assess the extracellular production efficiency of cbp21 and chi92, with gl ...201728884316
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