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the usefulness of molecular techniques to assess the presence of aeromonas spp. harboring virulence markers in foods.a total of 78 raw and 123 processed and ready-to-eat retail food samples were used to assess the presence of motile aeromonas spp. harboring virulence genes (cytotoxic enterotoxin and hemolysin genes) using a recently described pcr method in comparison with the conventional cultivation method based on the use of ampicillin-dextrin agar (ada) medium. with the ada-based method, 65/201 (32.3%) samples showed presumptive aeromonas spp. colonies whereas the pcr method revealed the presence of aeromon ...200415193799
over-expression, purification, and characterization of metallo-beta-lactamase imis from aeromonas veronii bv. sobria.the gene from aeromonas veronii bv. sobria encoding the metallo-beta-lactamase imis was subcloned into pet-26b, and imis was over-expressed in bl21(de3) escherichia coli and purified using sp-sepharose chromatography. this protocol yielded over 5 mg of imis per liter of growth culture under optimum conditions. the biochemical properties of recombinant imis were compared with those of native imis. recombinant and native imis have the same n-terminus of a-g-m-s-l, and cd spectroscopy was used to s ...200415249050
phylogenetic analysis of the genus aeromonas based on two housekeeping genes.the phylogenetic relationships of all known species of the genus aeromonas, and especially aeromonas bestiarum and aeromonas salmonicida, were investigated on 70 strains using the rpod sequence, which encodes the sigma70 factor. this analysis was complemented with the sequence of gyrb, which has already proven useful for determining the phylogenetic relationships in the genus. nucleotide sequences of rpod and gyrb showed that both genes had similar substitution rates (< 2 %) and a similar number ...200415388703
prevalence, serotype distribution, antibiotic susceptibility and genetic profiles of mesophilic aeromonas species isolated from hospitalized diarrhoeal cases in kolkata, india.a comprehensive study was performed to examine incidence, species distribution, drugs sensitivity, virulence genes and molecular fingerprints of aeromonas species isolated from patients with acute diarrhoea over a period of 2 years in kolkata, india. following the aerokey ii scheme, more than 95 % of strains were identified to species level. seven different species were encountered in this study, with aeromonas caviae being dominant, followed by aeromonas hydrophila and aeromonas veronii biovar ...200415150333
studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis.to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ...200415210998
an aeromonas veronii biovar sobria infection with disseminated intravascular gas production.we report a case of aeromonas veronii biovar sobria infection with disseminated intravascular gas production. the patient was an afebrile 15-year-old girl who had been quite healthy until the onset of the illness. she came to the hospital because of a 6-h history of increasing pain and swelling in her left thigh. on admission, no infection was suspected, and a tentative diagnosis of a ruptured left gracilis muscle was made. because the pain increased continuously, the treatment concentrated on p ...200414991516
type iii secretion system genes in clinical aeromonas isolates.we have identified the genes ascf and ascg, which encode components of a putative type iii secretion system (ttss) in aeromonas: we investigated the distribution of these and other ttss genes in 84 clinical isolates and found hybridizing sequences in 50% of the strains, with a higher prevalence in aeromonas hydrophila and aeromonas veronii than in aeromonas caviae.200415004096
adhesive properties of a lamb-like outer-membrane protein and its contribution to aeromonas veronii adhesion.to identify and characterize nonfimbrial proteins from aeromonas veronii involved in the attachment to epithelial cells in vitro.200415012808
aerolysin is activated by metalloprotease in aeromonas veronii biovar sobria.aeromonas veronii is an opportunistic human pathogen that causes diarrhoea and extraintestinal infections, such as wound infection and septicaemia. an a. veronii protease (avp) from a biovar sobria strain, aeg1, was partially purified and characterized. mature avp hydrolysed casein but not elastin, and protease activity was inhibited by metalloprotease inhibitors. nucleotide sequence analysis showed that avp belongs to the thermolysin family of proteases. an avp-deficient mutant was constructed ...200415150325
first record of the rare species aeromonas culicicola from a drinking water supply.we describe the recovery of the rare species aeromonas culicicola, so far known only in mosquitoes in india, from a drinking water supply in spain. typing, using enterobacterial repetitive intergenic consensus-pcr, revealed that the 27 new isolates belonged to 3 very closely related strains. these strains were genetically identified by 16s rrna gene sequencing. spanish strains differed from the mosquito strains in three nucleotide positions. the ahcytoen gene was present in these water strains, ...200515640231
identification of aeromonas culicicola by 16s rdna rflp.studies were conducted on the improvement of a. culicicola identification. this species is phenotypically very similar to a. veronii biotype sobria, a. sobria, and a. allosaccharophila. the sequences of 16s rdna of a. culicicola isolates show the highest similarity with a. jandaei, a. veronii, and a. caviae. digestion of 16s rdna pcr product with alui and mboi restriction endonucleases allowed discriminating a. culicicola from all other aeromonas species with the exception of a. jandaei. additio ...200516599307
aeromonas culicicola pidiyar et al. 2002 is a later subjective synonym of aeromonas veronii hickman-brenner et al. 1987.we re-investigated the taxonomic affiliation between aeromonas culicicola pidiyar et al. 2002 and aeromonas veronii hickman brenner et al. 1987, two species that have previously been shown to be closely related on the basis of gyrb and rpod gene sequencing and amplified fragment length polymorphism fingerprinting. from extended biochemical characterization and fatty acid analysis, it was concluded that a. culicicola did not constitute a unique phenotypic group in the genus aeromonas but instead ...200516156118
evolutionary and diagnostic implications of intragenomic heterogeneity in the 16s rrna gene in aeromonas strains.sequencing 16s rrna genes (ssu) cloned from aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. the ssu copies from aeromonas veronii lmg13695 clustered with sequences from four aeromonas species. these results demonstrate intragenomic heterogeneity of ssu and suggest caution when using ssu to identify aeromonads.200516159790
the major aeromonas veronii outer membrane protein: gene cloning and sequence analysis.the gene encoding the major outer membrane protein (omp) from aeromonas veronii, omp38, was cloned and characterized. sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20-amino-acid signal peptide at the n-terminal and the consensus sequence ala-x-ala (ala-asn-ala) as the signal peptidase i recognition site. the mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 da. the degree of iden ...200516252131
bulgecin a: a novel inhibitor of binuclear metallo-beta-lactamases.bulgecin a, a sulphonated n-acetyl-d-glucosamine unit linked to a 4-hydroxy-5-hydroxymethylproline ring by a beta-glycosidic linkage, is a novel type of inhibitor for binuclear metallo-beta-lactamases. using steady-state kinetic analysis with nitrocefin as the beta-lactam substrate, bulgecin a competitively inhibited the metallo-beta-lactamase bceii from bacillus cereus in its two-zinc form, but failed to inhibit when the enzyme was in the single-zinc form. the competitive inhibition was restore ...200515569001
freshwater selenium-methylating bacterial thiopurine methyltransferases: diversity and molecular phylogeny.the diversity of bacterial thiopurine methyltransferases (btpmt) among five natural se-methylating freshwaters was investigated by polymerase chain reaction (pcr) screenings and sequencings. dna sequence analyses confirmed the cloned products' identity and revealed a broad diversity of freshwater tpmts. neighbour-joining (nj) phylogenetic analyses combining these sequences, all genbank entries closely related to these sequences and deduced tpmts obtained in this work from selected gamma-proteoba ...200515658983
evaluation of dna vaccination of spotted sand bass (paralabrax maculatofasciatus) with two major outer-membrane protein-encoding genes from aeromonas veronii.genes encoding two major outer membrane proteins (omps) of the bacterial pathogen aeromonas veronii, omp38 and omp48, were used to construct dna vaccines. the protective effect of such vaccines against motile aeromonad septicaemia was evaluated in spotted sand bass (paralabrax maculatofasciatus), an endemic species of the mexican northwest pacific coast and a potential resource for the aquaculture industry. weak protein expression, as determined by immunoblotting, was observed after transfection ...200515752654
spectroscopic studies on cobalt(ii)-substituted metallo-beta-lactamase imis from aeromonas veronii bv. sobria.in an effort to probe the structure of a group bb metallo-beta-lactamase, co(ii)-substituted imis was prepared and characterized by electronic absorption, nmr, and epr spectroscopies. imis containing 1 equiv of co(ii) (co(ii)(1)-imis) was shown to be catalytically active. electronic absorption studies of co(ii)(1)-imis revealed the presence of two distinct features: (1) an intense sulfur to co(ii) ligand to metal charge transfer band and (2) less intense, co(ii) ligand field transitions that sug ...200515794654
new gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts.many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant amazonian leech, haementeria ghilianii, and two others from placobdelloides species. these hosts each possess a different mycetome or esophageal organ morphology wher ...200516151107
the chemical use, abuse, and dependence scale (cuad). rationale, reliability, and validity.this article describes the rationale for the development of the chemical use, abuse, and dependence scale (cuad). the instrument is in a semistructured interview format; it derives both substance use severity scores and dsm-iii-r substance use disorder diagnoses and can be administered in a short period with minimal training. the reliability and validity of the cuad are reported and appear satisfactory. the cuad is recommended for use as a detection, diagnostic, and treatment selection index in ...20051317464
male transsexualism in england: sixteen cases with surgical intervention.the syndrome of transsexualism is now regarded as a dinstinct entity separate from transvestism and homosexuality (benjamin, 1966; green and money, 1969). it refers to those patients who since childhood have been convinced of their wrong sex assignment and who wish to attain the sexual characteristics of the opposite sex and to live as a member of that sex. the present report is an initial one referring to the first group of transsexual patients studied at a postgraduate teaching hospital by a m ...20051169047
[personality of patients with myocardial infarct]. 20051236645
[surgical sciatica. study of 251 cases undergoing a single operation (author's transl)]. 2005544204
geriatric patient care. opinions of a sample of nurses. 20051134987
the differentiation of cytotoxic t lymphocytes in vitro: an ultrastructural study.t killer cells were identified morphologically in two different culture systems. the first system allowed identification by comparing under identical culture conditions two types of stimulation, one leading to cytotoxic activity levels that were very high (h-2 stimulation) and the other to levels which were practically nil (m-locus stimulation). a second system allowed identification by the use of a collaborative system in which relatively pure precursors of t killer cells were mixed with precur ...20051082798
[the effect of antibiotics on the intestinal flora]. 20061762418
changes in drug treatment after discharge from hospital in geriatric patients.to ascertain changes in drug treatment of elderly patients after discharge from hospital and to identify areas of communication which may require improvement.20061393119
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
effects of converting-enzyme inhibition on barrier function in diabetic glomerulopathy.differential solute clearances were used to examine the effects of a 90-day course of enalapril on glomerular barrier function in 16 proteinuric patients with diabetic glomerulopathy. by day 90, plasma renin and prorenin became elevated, and arterial pressure declined. transglomerular passage of dextrans of broad size distribution (radii 28-60 a) was lowered significantly. in a subset of 8 patients, withdrawal of enalapril was followed after an additional 30 days by a return of renin levels and ...20061698674
effects of converting-enzyme inhibition on barrier function in diabetic glomerulopathy.differential solute clearances were used to examine the effects of a 90-day course of enalapril on glomerular barrier function in 16 proteinuric patients with diabetic glomerulopathy. by day 90, plasma renin and prorenin became elevated, and arterial pressure declined. transglomerular passage of dextrans of broad size distribution (radii 28-60 a) was lowered significantly. in a subset of 8 patients, withdrawal of enalapril was followed after an additional 30 days by a return of renin levels and ...20061698674
calcium carbonate powder as a phosphate binder.with increasing recognition of problems regarding the use of aluminum hydroxide as a phosphate binder, calcium carbonate has become the medication of choice. use of calcium has, however, frequently been associated with development of hypercalcemia. at this institution, calcium carbonate powder as a phosphate binder, examination of its efficacy, and the frequency of hypercalcemia with its use were of great interest. calcium carbonate powder (calcarb-hd, 2.4 gms elemental calcium/packet) (calcarb- ...20062597473
changes in body composition and energy expenditure after six weeks' growth hormone treatment. 20061796983
changes in body composition and energy expenditure after six weeks' growth hormone treatment. 20061796983
glycosidase activities in gingival crevicular fluid in subjects with adult periodontitis or gingivitis.specific glycosidase activities were determined in samples of gingival crevicular fluid (gcf) collected from eight predetermined sites in two groups, each of 20 adult patients, with either gingivitis or periodontitis. the total activities (as units of enzyme activity per sample) of alpha-l-fucosidase, sialidase, beta-n-acetylglucosaminidase, beta-galactosidase, beta-glucosidase and alpha-glucosidase were significantly greater in the periodontitis group. in contrast, the total beta-mannosidase an ...20061610303
haemorheology and exercise: intrinsic flow properties of blood in marathon running.haemorheological and haematological parameters were studied in venous blood samples taken from 8 endurance-trained athletes before and after a marathon run. viscometry was performed in a 20 microns glass capillary and in a couette viscometer. apparent blood viscosity was lower in the capillary than in the rotational viscometer, but significant differences between pre- and post-run samples were not observed in either system. this is presumably due to the absence of changes of haematocrit (pre-run ...20061459744
[body packing as a fatal risk--results of a questionnaire survey in western europe].a questionnaire was sent to 138 institutes of forensic medicine in 18 countries of western europe to obtain information concerning bodypackers, who had died between 1980 and 1990. the total number of dead was 17 (4 own cases). 8 cases were reported from the federal republic of germany, 4 from italy, 2 from great britain and 1 from austria, spain and norway each. in 15 cases some circumstances were told. most of the bodypackers had transported heroin (10), in 2 cases cocaine and amphetamine were ...20061489327
[photic maculopathy by low energy laser beam. a case report].the authors report a case of photic maculopathy that developed in a 30-year-old laboratory worker after repeated, consecutive exposure to low energy he-ne laser. pigmented foveal change and a small central scotoma in the right eye with window defect on fluorescein angiography characterized this maculopathy. photochemical damage is considered to be the cause of the maculopathy.20061797372
[photic maculopathy by low energy laser beam. a case report].the authors report a case of photic maculopathy that developed in a 30-year-old laboratory worker after repeated, consecutive exposure to low energy he-ne laser. pigmented foveal change and a small central scotoma in the right eye with window defect on fluorescein angiography characterized this maculopathy. photochemical damage is considered to be the cause of the maculopathy.20061797372
mechanistic studies on the mononuclear znii-containing metallo-beta-lactamase imis from aeromonas sobria.in an effort to understand the reaction mechanism of a b2 metallo-beta-lactamase, steady-state and pre-steady-state kinetic and rapid freeze quench electron paramagnetic resonance (epr) studies were conducted on imis and its reaction with imipenem and meropenem. ph dependence studies revealed no inflection points in the ph range of 5.0-8.5, while proton inventories demonstrated at least 1 rate-limiting proton transfer. site-directed mutagenesis studies revealed that lys224 plays a catalytic role ...200616939225
prevalence of environmental aeromonas in south east queensland, australia: a study of their interactions with human monolayer caco-2 cells.to investigate the prevalence of aeromonas in a major waterway in south east queensland, australia, and their interactions with a gut epithelial model using caco-2 cells.200616968308
updated phylogeny of the genus aeromonas.recent phylogenetic studies of the genus aeromonas based on gyrb and rpod gene sequences have improved the phylogeny based on 16s rrna gene sequences first published in 1992, particularly in the ability to split closely related species. these studies did not include the recently described species aeromonas simiae and aeromonas molluscorum and only a single strain of aeromonas culicicola was available for analysis at that time. in the present work, these aeromonas species and newly isolated strai ...200617012583
biochemical and molecular characterization of tetracycline-resistant aeromonas veronii isolates from catfish.eighty-one tetracycline-resistant aeromonas sp. strains were isolated from farm-raised catfish. morphological and biochemical characteristics indicated that 23 of the 81 aeromonads were aeromonas hydrophila, 7 isolates were aeromonas trota, 6 isolates were aeromonas caviae, 42 isolates were aeromonas veronii, and 3 isolates were aeromonas jandaei. however, the alui and mboi restriction fragment length polymorphism (rflp) patterns of the pcr-amplified 1.4-kb 16s rrna gene from all 81 tetracycline ...200617021193
x-ray absorption spectroscopy of the zinc-binding sites in the class b2 metallo-beta-lactamase imis from aeromonas veronii bv. sobria.x-ray absorption spectroscopy was used to investigate the metal-binding sites of imis from aeromonas veronii bv. sobria in catalytically active (1-zn), product-inhibited (1-zn plus imipenem), and inactive (2-zn) forms. the first equivalent of zinc(ii) was found to bind to the consensus zn(2) site. the reaction of 1-zn imis with imipenem leads to a product-bound species, coordinated to zn via a carboxylate group. the inhibitory binding site of imis was examined by a comparison of wild-type imis w ...200617087519
detection of conjugation related type four secretion machinery in aeromonas culicicola.background: aeromonas sp. can now be considered relatively common enteropathogens due to the increase of diseases in humans. aeromonas culicicola is a gram negative rod-shaped bacterium isolated for the first time from the mosquito mid-gut, but subsequently detected in other insects and waters also. our previous study discovered that a. culicicola harbors three plasmids, which we designated as pac3249a, pac3249b and pac3249c. we investigated and report here the existence and genetic organization ...200617205119
[rapidly progressive myonecrosis by aeromonas veronii biotype sobria].myonecrosis due to aeromonas spp is exceptional. we report the case of a diabetic patient with liver cirrhosis who developed a rapidly progressive myonecrosis by aeromonas veronii biotype sobria. the portal of entry was an injury after falling down in an irrigation canal. the outcome was not favourable and surgical amputation of left leg was performed in spite of antibiotic treatment with cefotaxime and tobramicin. aeromonas spp can be very aggressive and this microorganism should be considered ...200617222071
molecular requirements for the colonization of hirudo medicinalis by aeromonas veronii. 200616623399
[aeromonas associated with an acute diarrhea outbreak in são bento do una, pernambuco].an acute diarrhea outbreak, with 2170 cases, was described during january to july, 2004, in são bento do una, pernambuco. 582 stools were examined and an enteric pathogen was recovered in 25% (145 patients). aeromonas species were the most frequent (114-19.5%) and the main isolates were aeromonas caviae (57-9.8%), aeromonas veronii biovar sobria (23-3.9%), aeromonas veronii biovar veronii (15-2.6%) and other species (19-3.2%). the other isolated enteropathogens were vibrio cholerae o1-ogawa toxi ...200616699653
rabbit meat as a source of bacterial foodborne pathogens.even though worldwide production of rabbit meat is >1,000,000 tons, little information is available for rabbit meat microbiology. this study provides data on the prevalence of salmonella, escherichia coli o157:h7, yersinia enterocolitica, listeria spp., motile aeromonas spp., and staphylococcus aureus on rabbit meat. a total of 24 rabbit carcasses from two abattoirs and 27 rabbit meat packages from supermarket displays were examined. in addition to culturing methods, associated virulence genes w ...200616715811
segmental ascending colitis associated with aeromonas veronii biovar sobria. 200616732806
culture-independent characterization of the digestive-tract microbiota of the medicinal leech reveals a tripartite symbiosis.culture-based studies of the microbial community within the gut of the medicinal leech have typically been focused on various aeromonas species, which were believed to be the sole symbiont of the leech digestive tract. in this study, analysis of 16s rrna gene clone libraries confirmed the presence of aeromonas veronii and revealed a second symbiont, clone pw3, a novel member of the rikenellaceae, within the crop, a large compartment where ingested blood is stored prior to digestion. the diversit ...200616820471
purification and characterization of cytolytic toxins produced by aeromonas hydrophila and aeromonas veronii biotype sobria strains.cytolytic toxins produced by aeromonas hydrophila and aeromonas veronii biotype sobria strains were partially purified from culture filtrates by two steps of purification: ammonium sulfate precipitation and hydrophobic chromatography using phenyl-sepharose cl-4b. hemolytic activity was detected in one or two peaks in elution profile. purified toxins were also cytotoxic to vero and cho cells. moreover, these toxins revealed cytotonic activity to cho cells.200616878602
[postoperative infection of an epigastric hematoma caused by aeromonas veronii biovar sobria].a 52-year-old man with a gastric signet-ring cell carcinoma but without acute symptoms was admitted for reconstructive surgery of the gastrointestinal tract.200617109273
cloning of atp-dependent protease clpxp genes in aeromonas veronii. 200617169116
sequential binding of cobalt(ii) to metallo-beta-lactamase ccra.in an effort to probe co(ii) binding to metallo-beta-lactamase ccra, epr, exafs, and (1)h nmr studies were conducted on ccra containing 1 equiv (1-co(ii)-ccra) and 2 equiv (co(ii)co(ii)-ccra) of co(ii). the epr spectra of 1-co(ii)-ccra and co(ii)co(ii)-ccra are distinct and indicate 5/6-coordinate co(ii) ions. the epr spectra also reveal the absence of significant spin-exchange coupling between the co(ii) ions in co(ii)co(ii)-ccra. exafs spectra of 1-co(ii)-ccra suggest 5/6-coordinate co(ii) wit ...200616430228
aeromonas veronii biovar sobria bacteraemia with septic arthritis confirmed by 16s rdna pcr in an immunocompetent adult.an 81-year-old man developed septic arthritis and bacteraemia with aeromonas veronii biovar sobria. cultures of the joint wash-out fluid were negative; however, dna matching that of aeromonas veronii was identified by 16s rdna pcr. the patient was successfully treated with a 4 week course of ciprofloxacin. no recognized risk factors were found.200616434719
culturable and vbnc vibrio cholerae: interactions with chironomid egg masses and their bacterial population.vibrio cholerae, the etiologic agent of cholera, is autochthonous to various aquatic environments. recently, it was found that chironomid (nonbiting midges) egg masses serve as a reservoir for the cholera bacterium and that flying chironomid adults are possible windborne carriers of v. cholerae non-o1 non-o139. chironomids are the most widely distributed insect in freshwater. females deposit egg masses at the water's edge, and each egg mass contains eggs embedded in a gelatinous matrix. hemagglu ...200717186156
phylogenetic identification of aeromonas strains isolated from carcasses of pig as new members of the species aeromonas allosaccharophila.the first description of the species aeromonas allosaccharophila was only based on two strains (the type strain cect4199, and a duplicate cect4200) isolated from diseased elvers (anguilla anguilla) of an eel-farm located in valencia, spain, and one stool isolate (atcc35942) from a female with diarrhoea and food poisoning in south carolina, u.s.a. in the present study, 17 aeromonas isolates obtained from carcasses of pigs and from the equipment for the cleaning process, and one strain recently is ...200717080292
importance of flagella and enterotoxins for aeromonas virulence in a mouse model.a genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaa/flab, flag), lateral flagella (lafa), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. when 16 aeromonas hydrophila strains, seven aeromonas veronii strains, and seven aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal inj ...200717496975
severe diarrhoea caused by aeromonas veronii biovar sobria in a patient with metastasised gist.this report describes the isolation of aeromonas veronii biovar sobria as the causative enteropathogen of diarrhoea in an oncological patient after failure of detection of other infectious agents. the case points out the severe and long course of the infection, the diagnostic dilemma, and the prompt recovery after antibiotic treatment.200718254499
a distinct cause of necrotizing fasciitis: aeromonas veronii biovar sobria.aeromonas veronii biovar sobria (veronii var. sobria) has not been implicated in the pathogenesis of necrotizing fasciitis in north america.200717999586
[identification of closely related bacteria via phylogenetic methods].to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences.200718067224
aeromonas veronii, a tributyltin (tbt)-degrading bacterium isolated from an estuarine environment, ria de aveiro in portugal.organotin compounds are used in a variety of industrial processes therefore their subsequent discharge into the environment is widespread. bacteria play an important role in biogeochemical transformations acting as natural decontamination agents. therefore, screening for tributyltin (tbt)-resistant and -degrading bacteria is relevant for the selection of isolates with decontamination ability of these polluted areas. with this purpose, 50 strains were isolated from sediment and water from ria de ...200717719084
[isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba].extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ...200717554439
induction of apoptosis in vero cells by aeromonas veronii biovar sobria vacuolating cytotoxic factor.recently, a cytotoxin named vacuolating cytotoxic factor (vcf) in aeromonas sobria and aeromonas veronii biovar sobria was described. we have now purified this factor using ion metallic affinity chromatography. the vcf is a nonhemolytic enterotoxin that acts as a serine protease. the toxin can be partially neutralized by serum antiaerolysin and it induced not only cytoplasmatic vacuole formation, but also mitochondrial disorders that must be signaling the apoptotic pathways, leading to vero (afr ...200717286562
spatial and temporal population dynamics of a naturally occurring two-species microbial community inside the digestive tract of the medicinal leech.the medicinal leech, hirudo verbana, is one of the simplest naturally occurring models for digestive-tract symbioses, where only two bacterial species, aeromonas veronii bv. sobria (gamma-proteobacteria) and a rikenella-like bacterium (bacteroidetes), colonize the crop, the largest compartment of the leech digestive tract. in this study, we investigated spatial and temporal changes of the localization and microcolony structure of the native symbionts in the crop, after ingestion of a sterile blo ...200717277211
aeromonas hemolytic uremic syndrome. a case and a review of the literature.although rarely, hemolytic uremic syndrome can be induced by aeromonas. we report a case in a 40-year-old spanish female produced by aeromonas veronii bv. sobria and review the previous cases described in the literature. this is the 2nd case described in adults.200717300902
interaction between innate immune cells and a bacterial type iii secretion system in mutualistic and pathogenic associations.animals house a community of bacterial symbionts in their digestive tracts that contribute to their well being. the medicinal leech, hirudo verbana, has a remarkably simple gut population carrying two extracellular microbes in the crop where the ingested blood is stored. this simplicity renders it attractive for studying colonization factors. aeromonas veronii, one of the leech symbionts, can be genetically manipulated and is a pathogen of mammals. screening transposon mutants of a. veronii for ...200717517651
use of the novel phylogenetic marker dnaj and dna-dna hybridization to clarify interrelationships within the genus aeromonas.the interrelationships of 27 aeromonas strains were investigated using dnaj sequences and dna-dna hybridization. dnaj sequence similarities showed a stronger relationship with dna-dna relatedness values than did 16s rrna gene sequence similarities. additionally, dnaj sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16s rrna gene sequences for the differentiation of strains at the species level. relationships among aeromonas species were theref ...200717551035
the role of c-di-gmp signaling in an aeromonas veronii biovar sobria strain.aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. it is shown that all isolates of persistent aeromonas clones show strong biofilm formation ability. c-di-gmp regulates biofilm formation in many bacteria. to investigate the impact of c-di-gmp signaling, we introduced heterologous ggdef and eal domain proteins from salmonella typhimurium to an aeromonas veronii biovar sobria strain. overexpression of the ggdef domain protein adra increased c-di-gmp concentration ...200717573931
convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory.although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ...200717596358
identification of aeromonas veronii genes required for colonization of the medicinal leech, hirudo verbana.most digestive tracts contain a complex consortium of beneficial microorganisms, making it challenging to tease apart the molecular interactions between symbiont and host. the digestive tract of hirudo verbana, the medicinal leech, is an ideal model system because it harbors a simple microbial community in the crop, comprising the genetically amenable aeromonas veronii and a rikenella-like bacterium. signature-tagged mutagenesis (stm) was used to identify genes required for digestive tract colon ...200717616592
characterization of a catalase gene from aeromonas veronii, the digestive-tract symbiont of the medicinal leech.the catalase gene kata of the medicinal leech symbiont aeromonas veronii bv. sobria was cloned, sequenced, and functionally characterized. southern hybridization, using an a. veronii kata-specific hybridization probe, suggested the presence of a single gene copy in many aeromonas species. a. veronii kata consisted of 1446 nt encoding a protein with a high degree of similarity to the small-subunit group iii bacterial catalases. a catalase-null mutant (jg186) was constructed through gene-replaceme ...200717526846
virulence potential and genetic diversity of aeromonas caviae, aeromonas veronii, and aeromonas hydrophila clinical isolates from mexico and spain: a comparative study.a comparative study of 109 aeromonas clinical isolates belonging to the 3 species most frequently isolated from patients with diarrhea in mexico and spain was performed to investigate the distribution of 3 prominent toxin genes and the gene encoding flagellin of lateral flagella; 4 well-established virulence factors in the genus aeromonas. the aerolysin-hemolysin toxin genes were the most prevalent, being present in 89% of the total isolates. the ast toxin gene was conspicuously absent from the ...200717898843
tracheobronchitis caused by aeromonas veronii biovar sobria after near-drowning.a 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of aeromonas sp. in the water. an isolate of aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. the causality was supported by the massive growth of a. veronii in bronchial secretion, the presence of a type iii secretion system in the bacterial isolate, ...200717965361
epidemiological surveys of senile dementia in japan.epidemiological surveys of senile dementia at home were carried out on 3,754 subjects for two consecutive years, 1987 and 1988. the prevalence and incidence rates were 4.1% and 1.01%, respectively. concerning the incidence rate, the sample populations of previous reports were small in number--less than 1,000. if we suppose that the incidence were to be approximately 1%, then it follows that dementia would be noted yearly in no more than one out of 100 senile subjects. a difference in subject num ...20072045242
[a method of administration of drugs into the small joints of the hand and foot]. 20071925267
fixed drug eruption due to pipemidic acid.a 71-year-old woman with fixed drug eruption caused by pipemidic acid is reported. the patient developed typical lesions on rechallenge with pipemidic acid, but not with nalidixic acid, an analogue of pipemidic acid.20071828818
conservative surgery and radiation therapy for early stage breast cancer. can large trial experiences be reproduced in a community hospital setting?conservative surgery and radiation therapy were used to treat 212 patients with ajc clinical stage i or ii breast carcinoma at the baptist hospital of miami. all had lumpectomy and most axillary lymph node dissection, followed by breast irradiation to a dose of 45 gy and a boost dose of 14 to 16 gy to the surgical bed. median follow-up was 55 months. the five-year actuarial local control and survival rates were 94% and 86% respectively. eighty-six percent of the patients had excellent or good co ...20071963436
dna adducts in bronchial biopsies.to investigate the feasibility of measuring dna-carcinogen adducts in the lungs of non-surgical patients, endobronchial biopsies were obtained from 78 patients undergoing routine diagnostic bronchoscopy. lung cancer was present in 37 (47%) of the patients. dna was isolated from the tissues and analyzed by hplc- or nuclease-pi-enriched 32p-postlabelling, using procedures selective for aromatic adducts. chromatograms from all 28 current smokers showed a distinctive diagonal adduct zone which was p ...20072045196
does prestretching stainless-steel wire have any clinical significance?clinical parameters of prestretch and tension forces placed on stainless-steel wire by 20 oral and maxillofacial surgeons were obtained. unstretched wire was clinically as tough or tougher than prestretched wire of a similar gauge. both prestretched and unstretched wires, twisted to their maximum, loosened when under tension. as the prestretch forces increased, the clinical strength of the wire diminished.20071890520
fournier's gangrene.fournier's gangrene or synergistic gengrene of scrotum and penis is a relatively rare but serious condition which can occur in any general or hospital practice. early diagnosis with prompt aggressive treatment including antibiotics and surgical debridement are necessary to achieve patient survival. we report our clinical experience with three cases which occurred in three different hospitals in the irish republic. in two of these cases diabetes mellitus was an important predisposing factor. all ...20072045267
a double-blind trial of essential fatty acid supplementation in patients with tardive dyskinesia.this study reports the results of a trial of essential fatty acid (efa) supplementation in psychiatric patients (predominantly schizophrenics) with movement disorders. evidence of efa deficiency in these patients was observed. the antidyskinetic effect of efa supplementation was marginally significant but not clinically important. however, active treatment produced highly significant improvements in total psychopathology scores and schizophrenia subscale scores, and a significant improvement in ...20082565585
enrolled nurses. we're backing the charter! 20082587373
[experimental studies in the separation and identification of ingredients of the hexachlorocyclohexane ointment "antiscabiosum" (purity assessment)]. 20082444036
complications of intracranial bypass for vertebrobasilar insufficiency.the authors' initial experience with intracranial revascularization of vertebrobasilar insufficiency, reported previously, fortunately yielded reasonably good results with high patency rates but in some cases there were significant, albeit temporary, complications. since that time, major brain-stem strokes have occurred in two patients following superficial temporal to superior cerebellar artery bypass procedures. this occurrence has caused the authors to reassess their experience with this proc ...20082643687
complications of intracranial bypass for vertebrobasilar insufficiency.the authors' initial experience with intracranial revascularization of vertebrobasilar insufficiency, reported previously, fortunately yielded reasonably good results with high patency rates but in some cases there were significant, albeit temporary, complications. since that time, major brain-stem strokes have occurred in two patients following superficial temporal to superior cerebellar artery bypass procedures. this occurrence has caused the authors to reassess their experience with this proc ...20082643687
trends in screening mammograms for women 50 years of age and older--behavioral risk factor surveillance system, 1987. 20082493130
methyl 2-[4-(2-chloroethyl)-2,3-dihydro-7-nitroquinoxalin-1-yl]benzoate from intramolecular cyclization of a nitroaromatic mustard.c18h18cin3o4, mr = 375.81, orthorhombic, p212121, a = 11.000 (3), b = 8.023 (2), c = 19.316 (5) a, v = 1704.68 a3, z = 4, dm = 1.46 (1), dx = 1.417 g cm-3, mo kalpha, lambda = 0.71069 a, mu = 1.82 cm-1, f(000) = 760, t = 293 (1) k, r = 0.051 for 985 [i greater than 2.5 sigma(i)] reflections. x-ray analysis confirms that the nitroaromatic mustard methyl n-(2-[bis(2-chloroethyl)amino]- 5-nitrophenyl)anthranilate undergoes rapid intramolecular cyclization, but the product is the 7-nitro rather than ...20082635608
non-germ cell testicular tumours in nigerians.non-germ cell testicular tumours are reported to be more common in peoples of negroid and asian descent than in caucasians. this group of tumours comprised seven of sixteen testicular tumours seen in a ten-year period at the university of benin teaching hospital, benin city, nigeria. however, unlike experiences elsewhere in africa and the west indies where paratesticular tumours accounted for a majority of these tumours, malignant lymphomas constituted over 70% of non-germinal testicular tumours ...20082635452
non-germ cell testicular tumours in nigerians.non-germ cell testicular tumours are reported to be more common in peoples of negroid and asian descent than in caucasians. this group of tumours comprised seven of sixteen testicular tumours seen in a ten-year period at the university of benin teaching hospital, benin city, nigeria. however, unlike experiences elsewhere in africa and the west indies where paratesticular tumours accounted for a majority of these tumours, malignant lymphomas constituted over 70% of non-germinal testicular tumours ...20082635452
case-control study on histologically determined multiple primary lung cancer.between 1965 and 1985, 72 (64 male and 8 female) cases of multiple primary lung cancer (mplc) in the national cancer center hospital, japan, were reviewed, and a retrospective hospital-based case-control study was performed with special reference to the patients' smoking and family histories. control cases were selected from patients with unicentric lung cancer (ulc) with a longer survival time than that for other patients with whom they were matched for age, sex, histologic examination of prima ...20083476786
[surgical methods in the treatment of patients with acute thrombosis of hemorrhoids]. 20082699115
detection of aerolysin gene in aeromonas hydrophila isolated from fish and pond water.aerolysin is a hemolytic toxin encoded by aerolysin gene (1482 bp) that plays a key role in the pathogenesis of aeromonas hydrophila infection in fish. new speciesspecific primers were designed to amplify 326 bp conserved region of aerolysin gene for a. hydrophila. twenty-five isolates of a. hydrophila recovered from fish and pond water were studied for detection of aerolysin gene. aerolysin gene was detected in 85% of the isolates during the study. the designed primers were highly specific and ...200823100746
conformational changes in the metallo-beta-lactamase imis during the catalytic reaction: an epr spectrokinetic study of co(ii)-spin label interactions.metallo-beta-lactamases are responsible for conferring antibiotic resistance on certain pathogenic bacteria. in consequence, the search for inhibitors that may be useful in combating antibiotic resistance has fueled much study of the active sites of these enzymes. there exists circumstantial evidence that the binding of substrates and inhibitors to metallo-beta-lactamases may involve binding to the organic part of the molecule, in addition to or prior to binding to one or more active site metal ...200818528987
[characterization of aeromonas spp isolates from newborns hospitalized].aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ...200818545840
characterization of vibrio cholerae isolated from the aquatic basins of the state of pernambuco, brazil.through a continuous bacteriological monitoring programme carried out by the health secretariat of the state of pernambuco, brazil, two isolates of vibrio cholerae o1 el tor ogawa were discovered in an endemic area in 2001, during a cholera inactive period, along with six v. cholerae non-o1/non-o139 strains and two aeromonas veronii biovar sobria strains showing an unusual characteristic of agglutination with o1 antiserum. between that time and 2005, eight other o1 isolates were found. the virul ...200818258274
effect of packaging atmosphere on the microbial attributes of pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c.effect of packaging atmosphere (air and under different modified atmospheres (mas), 40% co2/60% o2, 50%/50% o2, 60% co2/40% o2, 70% co2/30% o2 and 40% co2/30% o2/30% n2) on the microbial and biochemical attributes of fresh pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c was investigated. trimethylamine nitrogen (tma-n) and thiobarbituric acid (tba) values remained lower than the proposed acceptability limits throughout the storage period. results demonstrated that storage of pearls ...200818355677
[the use of imaging procedures following kidney transplantation--diagnostic strategy].despite the continuously increasing number of successfully transplanted kidneys, graft and patient are still "threatened" by several postoperative complications as acute and chronic rejection, acute tubular necrosis, urologic and vascular complications and disorders caused by immunosuppressive medication. an early and safe detection of these conditions is of high clinical importance and a challenge to diagnostic imaging. all radiologic methods and nuclear medicine have their value for transplant ...20082678232
[the use of imaging procedures following kidney transplantation--diagnostic strategy].despite the continuously increasing number of successfully transplanted kidneys, graft and patient are still "threatened" by several postoperative complications as acute and chronic rejection, acute tubular necrosis, urologic and vascular complications and disorders caused by immunosuppressive medication. an early and safe detection of these conditions is of high clinical importance and a challenge to diagnostic imaging. all radiologic methods and nuclear medicine have their value for transplant ...20082678232
on the kinetic depth effect.the problem of the kinetic depth effect is revisited. we study how many points in how many views are necessary and sufficient to recover structure. the constraints in the cases where the velocities of the image points are known, and the positions of the image points are known with the correspondence between them established, are different and they have to be studied separately. in the case of two projections of any number of points there are infinitely many solutions, but if we regularize the pr ...20082719982
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