aeromonas hemolytic uremic syndrome. a case and a review of the literature. | although rarely, hemolytic uremic syndrome can be induced by aeromonas. we report a case in a 40-year-old spanish female produced by aeromonas veronii bv. sobria and review the previous cases described in the literature. this is the 2nd case described in adults. | 2007 | 17300902 |
importance of flagella and enterotoxins for aeromonas virulence in a mouse model. | a genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaa/flab, flag), lateral flagella (lafa), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. when 16 aeromonas hydrophila strains, seven aeromonas veronii strains, and seven aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal inj ... | 2007 | 17496975 |
interaction between innate immune cells and a bacterial type iii secretion system in mutualistic and pathogenic associations. | animals house a community of bacterial symbionts in their digestive tracts that contribute to their well being. the medicinal leech, hirudo verbana, has a remarkably simple gut population carrying two extracellular microbes in the crop where the ingested blood is stored. this simplicity renders it attractive for studying colonization factors. aeromonas veronii, one of the leech symbionts, can be genetically manipulated and is a pathogen of mammals. screening transposon mutants of a. veronii for ... | 2007 | 17517651 |
characterization of a catalase gene from aeromonas veronii, the digestive-tract symbiont of the medicinal leech. | the catalase gene kata of the medicinal leech symbiont aeromonas veronii bv. sobria was cloned, sequenced, and functionally characterized. southern hybridization, using an a. veronii kata-specific hybridization probe, suggested the presence of a single gene copy in many aeromonas species. a. veronii kata consisted of 1446 nt encoding a protein with a high degree of similarity to the small-subunit group iii bacterial catalases. a catalase-null mutant (jg186) was constructed through gene-replaceme ... | 2007 | 17526846 |
use of the novel phylogenetic marker dnaj and dna-dna hybridization to clarify interrelationships within the genus aeromonas. | the interrelationships of 27 aeromonas strains were investigated using dnaj sequences and dna-dna hybridization. dnaj sequence similarities showed a stronger relationship with dna-dna relatedness values than did 16s rrna gene sequence similarities. additionally, dnaj sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16s rrna gene sequences for the differentiation of strains at the species level. relationships among aeromonas species were theref ... | 2007 | 17551035 |
[isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba]. | extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ... | 2007 | 17554439 |
the role of c-di-gmp signaling in an aeromonas veronii biovar sobria strain. | aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. it is shown that all isolates of persistent aeromonas clones show strong biofilm formation ability. c-di-gmp regulates biofilm formation in many bacteria. to investigate the impact of c-di-gmp signaling, we introduced heterologous ggdef and eal domain proteins from salmonella typhimurium to an aeromonas veronii biovar sobria strain. overexpression of the ggdef domain protein adra increased c-di-gmp concentration ... | 2007 | 17573931 |
convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory. | although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ... | 2007 | 17596358 |
identification of aeromonas veronii genes required for colonization of the medicinal leech, hirudo verbana. | most digestive tracts contain a complex consortium of beneficial microorganisms, making it challenging to tease apart the molecular interactions between symbiont and host. the digestive tract of hirudo verbana, the medicinal leech, is an ideal model system because it harbors a simple microbial community in the crop, comprising the genetically amenable aeromonas veronii and a rikenella-like bacterium. signature-tagged mutagenesis (stm) was used to identify genes required for digestive tract colon ... | 2007 | 17616592 |
aeromonas veronii, a tributyltin (tbt)-degrading bacterium isolated from an estuarine environment, ria de aveiro in portugal. | organotin compounds are used in a variety of industrial processes therefore their subsequent discharge into the environment is widespread. bacteria play an important role in biogeochemical transformations acting as natural decontamination agents. therefore, screening for tributyltin (tbt)-resistant and -degrading bacteria is relevant for the selection of isolates with decontamination ability of these polluted areas. with this purpose, 50 strains were isolated from sediment and water from ria de ... | 2007 | 17719084 |
[new species of aeromonas isolated in cuba]. | one hundred and fifty-five strains of aeromonas isolated in the stools of children under 5 years presenting with acute diarrheal disease were studied. using the aerokey ii system for the identification of species, 47 strains were identified as aeromonas caviae, 58 as aeromonas hydrophila, 23 as aeromonas veronii biovar sobria, 14 as aeromonas trota, 9 as aeromonas veronii biovar veronii, 2 as aeromonas jandaei and 2 as aeromonas shubertii, emphasis is placed on the advantages of this method whic ... | 1995 | 9813480 |
tracheobronchitis caused by aeromonas veronii biovar sobria after near-drowning. | a 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of aeromonas sp. in the water. an isolate of aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. the causality was supported by the massive growth of a. veronii in bronchial secretion, the presence of a type iii secretion system in the bacterial isolate, ... | 2007 | 17965361 |
a distinct cause of necrotizing fasciitis: aeromonas veronii biovar sobria. | aeromonas veronii biovar sobria (veronii var. sobria) has not been implicated in the pathogenesis of necrotizing fasciitis in north america. | 2007 | 17999586 |
[identification of closely related bacteria via phylogenetic methods]. | to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences. | 2007 | 18067224 |
aeromonas-acanthamoeba interaction and early shift to a viable but nonculturable state of aeromonas by acanthamoeba. | to investigate the hypothesis that amoeba may comprise a significant environmental reservoir for aeromonas, acanthamoeba-aeromonas interaction experiments were performed. | 2008 | 18194253 |
severe diarrhoea caused by aeromonas veronii biovar sobria in a patient with metastasised gist. | this report describes the isolation of aeromonas veronii biovar sobria as the causative enteropathogen of diarrhoea in an oncological patient after failure of detection of other infectious agents. the case points out the severe and long course of the infection, the diagnostic dilemma, and the prompt recovery after antibiotic treatment. | 2007 | 18254499 |
characterization of vibrio cholerae isolated from the aquatic basins of the state of pernambuco, brazil. | through a continuous bacteriological monitoring programme carried out by the health secretariat of the state of pernambuco, brazil, two isolates of vibrio cholerae o1 el tor ogawa were discovered in an endemic area in 2001, during a cholera inactive period, along with six v. cholerae non-o1/non-o139 strains and two aeromonas veronii biovar sobria strains showing an unusual characteristic of agglutination with o1 antiserum. between that time and 2005, eight other o1 isolates were found. the virul ... | 2008 | 18258274 |
effect of packaging atmosphere on the microbial attributes of pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c. | effect of packaging atmosphere (air and under different modified atmospheres (mas), 40% co2/60% o2, 50%/50% o2, 60% co2/40% o2, 70% co2/30% o2 and 40% co2/30% o2/30% n2) on the microbial and biochemical attributes of fresh pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c was investigated. trimethylamine nitrogen (tma-n) and thiobarbituric acid (tba) values remained lower than the proposed acceptability limits throughout the storage period. results demonstrated that storage of pearls ... | 2008 | 18355677 |
symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations. | hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. infections by aeromonas occur in up to 20% of patients unless a preemptive antibiotic treatment is administered. the associated infections demonstrate the need for a better understanding of the digestive tract flora of h. medicinalis. early studies reported the presence of a single bacterial species in the digestive tract and suggested that these bacteria were endosymbionts contributing to the digestion of bl ... | 1999 | 9864188 |
plasmid-mediated qnrs2 determinant from a clinical aeromonas veronii isolate. | | 2008 | 18505860 |
conformational changes in the metallo-beta-lactamase imis during the catalytic reaction: an epr spectrokinetic study of co(ii)-spin label interactions. | metallo-beta-lactamases are responsible for conferring antibiotic resistance on certain pathogenic bacteria. in consequence, the search for inhibitors that may be useful in combating antibiotic resistance has fueled much study of the active sites of these enzymes. there exists circumstantial evidence that the binding of substrates and inhibitors to metallo-beta-lactamases may involve binding to the organic part of the molecule, in addition to or prior to binding to one or more active site metal ... | 2008 | 18528987 |
[characterization of aeromonas spp isolates from newborns hospitalized]. | aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ... | 2008 | 18545840 |
functional characterization of type iv pili expressed on diarrhea-associated isolates of aeromonas species. | our past work has shown that long, flexible type iv pili (single or in bundles) are the predominant pili expressed on fecal isolates of diarrhea-associated species of aeromonas (aeromonas veronii biovar sobria and a. caviae). they represent a family of type iv pili which we have designated bfp (for bundle-forming pili). reports from japan suggest that bfp are intestinal colonization factors. this study presents compelling evidence to support this conclusion. aeromonas bacteria and/or bfp purifie ... | 1999 | 10496928 |
aeromonas spp. human isolates induce apoptosis of murine macrophages. | interactions of aeromonas caviae, aeromonas veronii biotype sobria, and aeromonas hydrophila strains isolated from fecal specimens of humans with gastroenteritis on murine macrophages, j774 cells, were investigated. analyses of cellular morphology and dna fragmentation in phagocytes infected with these strains exhibited typical characteristic features of cells undergoing apoptosis. we observed the morphological changes, including condensation of nuclear chromatin, formation of apoptotic bodies a ... | 2009 | 19005723 |
[severe pneumonia caused by aeromonas veronii biovar sobria: a case report and review of the literature]. | to describe the clinical features of severe pneumonitis with aeromonas veronii biovar sobria. | 2008 | 19080598 |
typing of clinical and environmental aeromonas veronii strains based on the 16s-23s rdna spacers. | genetic relationships of aeromonas veronii strains isolated from human and environmental sources were investigated by restriction fragment length polymorphism (rflp) of the polymerase chain reaction-amplified intergenic spacer region (isr) flanked by the 16s and 23s rrna genes. when using endonucleases alui, hinfi and cfoi the 16s-23s rdna-rflp patterns showed considerable overall similarity, although most strains yielded specific profiles. several intra-specific lines of descent comprised clini ... | 2000 | 10865175 |
development of imipenem resistance in an aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis. | several imipenem-susceptible and -resistant aeromonas veronii biovar sobria isolates with different morphologies and antimicrobial susceptibilities recovered from bile samples of a patient with cholangitis were analysed. these isolates belonged to the same clone and the imipenem-resistant strains showed overexpression of the imis gene, encoding a chromosomal carbapenemase. these results should make clinicians aware of the possible emergence of multidrug-resistant a. veronii biovar sobria, perhap ... | 2009 | 19273640 |
cloning and expression of an outer membrane protein ompw of aeromonas hydrophila and study of its distribution in aeromonas spp. | the main aims of this study were to clone and express an outer membrane protein (omp), ompw, of aeromonas hydrophila and to study its distribution in aeromonas spp. | 2009 | 19426281 |
new structural variants of homoserine lactones in bacteria. | n-acylhomoserine lactones (ahls) are used by a wide variety of bacteria for cell-cell communication in "quorum-sensing". these compounds are derived from l-homoserine lactone and a fatty acid, which varies in chain-length, degree of saturation, and the presence or absence of an oxygen atom at c-3. in this study we describe for the first time the occurrence of acyl chains carrying a methyl branch, and present a gc-ms-based method that can be used to distinguish these compounds from unbranched iso ... | 2009 | 19533714 |
prevalence of genes encoding the type three secretion system and the effectors aext and aexu in the aeromonas veronii group. | the prevalence of important virulence factors, the type iii secretion system (t3ss) and two t3ss-dependent toxins, aext and aexu, was evaluated in the aeromonas veronii group (avg). members of the avg have a broad host range, including vertebrates and invertebrates, and form a variety of associations, spanning from pathogenic to mutualistic. our avg strain collection consists of human, duck, eel, and leech isolates. these isolates were examined for the presence of the t3ss, aext, and aexu throug ... | 2009 | 19534604 |
aeromonas fluvialis sp. nov., isolated from a spanish river. | a gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(t), was isolated from a water sample collected from the muga river, girona, north-east spain. preliminary analysis of the 16s rrna gene sequence showed that this strain belonged to the genus aeromonas, the nearest species being aeromonas veronii (99.5 % similarity, with seven different nucleotides). a polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrb, rpod, reca, dnaj and g ... | 2010 | 19648345 |
plasmid-mediated qnrs2 determinant in an aeromonas caviae isolate recovered from a patient with diarrhoea. | a qnrs2 gene was identified in an aeromonas caviae isolate (mics of ciprofloxacin, norfloxacin and ofloxacin >32 mg/l) from a stool sample collected from a patient with gastroenteritis. the analysis of the gyra and parc genes revealed amino acid substitutions ser83-ile and ser80-thr, respectively. in addition, five out of 41 nalidixic acid-resistant aeromonas isolates studied (26 identified as aeromonas veronii bv sobria and 15 identified as a. caviae) showed ciprofloxacin resistance. the identi ... | 2010 | 19863591 |
epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping. | ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ... | 2000 | 10997832 |
[isolation and identification of pathogenic aeromonas veronii isolated from infected siberian sturgeon (acipenser baerii)]. | our study aimed at searching for the pathogenic factor causing siberian sturgeon (acipenser baerii) disease. | 2009 | 20069873 |
biochemical characterization of three putative atpases from a new type iv secretion system of aeromonas veronii plasmid pac3249a. | type four secretion systems (tfss) are bacterial macromolecular transport systems responsible for transfer of various substrates such as proteins, dna or protein-dna complexes. tfsss encode two or three atpases generating energy for the secretion process. these enzymes exhibit highest sequence conservation among type four secretion components. | 2010 | 20144229 |
identification of genes associated with copper tolerance in an adhesion-defective mutant of aeromonas veronii biovar sobria. | tnphoa mutagenesis was used to identify adhesins of aeromonas veronii biovar sobria 3767, a strain isolated from a diarrhoeal stool specimen. six mutants, from a library of 154, exhibited significantly reduced levels of adhesion to hep-2 cells. primers to the terminal regions of tnphoa were used for inverse pcr and the product from one mutant was cloned into pbluescript and partial sequence data obtained. scanning genbank and embl data bases revealed dna sequence similarity to the copa gene of p ... | 2000 | 11024350 |
ingested blood contributes to the specificity of the symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech. | hirudo medicinalis, the medicinal leech, usually carries in its digestive tract a pure culture of aeromonas veronii bv. sobria. such specificity is unusual for digestive tracts that are normally colonized by a complex microbial consortium. important questions for the symbiotic interaction and for the medical application after microvascular surgery are whether other bacteria can proliferate or at least persist in the digestive tract of h. medicinalis and what factors contribute to the reported sp ... | 2000 | 11055917 |
mucosal immune response of spotted sand bass paralabrax maculatofasciatus (steindachner, 1868) orally immunised with an extracellular lectin of aeromonas veronii. | to assess the immunogenic and immunoprotective role of the extracellular lectin from aeromonas veronii (mcbp), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (paralabrax maculatofasciatus) were orally immunised either with soluble mcbp, adjuvant-conjugated mcbp or immobilised mcbp on latex microspheres. the results suggest that the mcbp is capable of eliciting protective immunity against a. veronii infections when administe ... | 2001 | 11308074 |
new dna-dna hybridization and phenotypic data on the species aeromonas ichthiosmia and aeromonas allosaccharophila: a. ichthiosmia schubert et al. 1990 is a later synonym of a. veronii hickman-brenner et al. 1987. | previously, a dna fingerprinting study based on amplified fragment length polymorphism (aflp) analysis has revealed a possible genotypic resemblance of the species aeromonas ichthiosmia and aeromonas allosaccharophila to aeromonas veronii (huys et al., int. j. syst. bacteriol. 46, 572-580 [19961). currently, two genotypically indistinguishable biovars are known to exist in the latter species, namely a. veronii biovar sobria and a. veronii biovar veronii. in the current study, new dna-dna hybridi ... | 2001 | 11518320 |
differential regulation of caspase-1 activation via nlrp3/nlrc4 inflammasomes mediated by aerolysin and type iii secretion system during aeromonas veronii infection. | aeromonas spp. are gram-negative bacteria that cause serious infectious disease in humans. such bacteria have been shown to induce apoptosis in infected macrophages, yet the host responses triggered by macrophage death are largely unknown. in this study, we demonstrate that the infection of mouse bone marrow-derived macrophages with aeromonas veronii biotype sobria triggers activation of caspase-1 with the ensuing release of il-1β and pyroptosis. caspase-1 activation in response to a. veronii in ... | 2010 | 21037094 |
helicobacter pylori and other enteric bacteria in freshwater environments in mexico city. | helicobacter pylori infection is common in the mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear. | 2001 | 11578764 |
microbial populations of an upflow anaerobic sludge blanket reactor treating wastewater from a gelatin industry. | the microbial populations of an upflow anaerobic sludge blanket reactor, used for treating wastewater from the gelatin industry, were studied by microbiological methods and phase-contrast and electron microscopy. microscopy examination of the sludge showed a complex mixture of various rod-shaped and coccoid bacterial pluslong filaments and verymobile curved rods. in addition free-living anaerobic ciliates and flagellates were also observed. the trophic group population observed in decreasing ord ... | 2001 | 11873883 |
detection of aaca-aphd, qaced1, mara, flor, and teta genes from multidrug-resistant bacteria: comparative analysis of real-time multiplex pcr assays using evagreen(®) and sybr(®) green i dyes. | we have developed multiplex real-time pcr assays that utilize dna-intercalating dyes, sybr green i (sg) and evagreen (eg), with two primer sets (set 1=qaced1, teta and aaca-aphd; set 2=teta, mara, and flor) to simultaneously amplify the qaced1, teta, aaca-aphd, mara, and flor genes. validity of the multiplex pcr assays was confirmed by testing 83 bacterial isolates, including staphylococcus aureus (28 isolates), enterococcus spp. (17 isolates), salmonella enterica serovar typhimurium (8 isolates ... | 2011 | 21256956 |
complex evolutionary history of the aeromonas veronii group revealed by host interaction and dna sequence data. | aeromonas veronii biovar sobria, aeromonas veronii biovar veronii, and aeromonas allosaccharophila are a closely related group of organisms, the aeromonas veronii group, that inhabit a wide range of host animals as a symbiont or pathogen. in this study, the ability of various strains to colonize the medicinal leech as a model for beneficial symbiosis and to kill wax worm larvae as a model for virulence was determined. isolates cultured from the leech out-competed other strains in the leech model ... | 2011 | 21359176 |
occurrence, abundance and elimination of class 1 integrons in one municipal sewage treatment plant. | integrons are elements that encode a site-specific recombination system that recognizes and captures mobile gene cassettes and are closely related to multiple resistances of environmental microorganisms. this study was undertaken to determine the efficiency of an activated sludge process to remove integrons. the prevalence and characteristics of class 1 integrons were investigated for bacterial species isolated from the activated sludge of nanjing jiangxinzhou sewage treatment plant (stp, china) ... | 2011 | 21431316 |
aeromonas spp. induce apoptosis of epithelial cells through an oxidant-dependent activation of the mitochondrial pathway. | we investigated interactions of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila strains, isolated from faecal specimens of humans with gastroenteritis, with ht29 intestinal epithelial cells. all strains were found to be cytotoxic to the cells. bacterial infection caused generation of reactive oxygen species (ros) and nitric oxide radical (no(-à)). the maximal levels of ros and no(-à) were 14 and 35 times, respectively, greater in cells infected with aeromonas spp. tha ... | 2011 | 21436367 |
characterisation of aeromonas spp. isolated from frozen fish intended for human consumption in mexico. | a total of 82 strains of presumptive aeromonas spp. were identified biochemically and genetically (16s rdna-rflp). the strains were isolated from 250 samples of frozen fish (tilapia, oreochromis niloticus niloticus) purchased in local markets in mexico city. in the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. in addition, we studied the antimicrobial patterns of t ... | 2003 | 12781953 |
hemolytic and proteolytic activities of aeromonas hydrophila and aeromonas veronii biovar sobria in broth and salmon extract at different temperatures. | expression of hemolytic and proteolytic activities throughout the growth cycle was investigated with two enterotoxic aeromonad strains assigned to the species aeromonas hydrophila and aeromonas veronii biovar sobria. although growth kinetic data were dependent on strain, temperature, and substrate, maximum populations attained were higher than 9 log cfu/ml in aerated tryptone soya broth plus yeast extract (tsbye) and salmon extract within the range 4 to 28 degrees c. for both strains in tsbye, v ... | 2004 | 14968959 |
prevalence, serotype distribution, antibiotic susceptibility and genetic profiles of mesophilic aeromonas species isolated from hospitalized diarrhoeal cases in kolkata, india. | a comprehensive study was performed to examine incidence, species distribution, drugs sensitivity, virulence genes and molecular fingerprints of aeromonas species isolated from patients with acute diarrhoea over a period of 2 years in kolkata, india. following the aerokey ii scheme, more than 95 % of strains were identified to species level. seven different species were encountered in this study, with aeromonas caviae being dominant, followed by aeromonas hydrophila and aeromonas veronii biovar ... | 2004 | 15150333 |
complete genome sequence of aeromonas veronii strain b565. | aeromonas veronii strain b565 was isolated from aquaculture pond sediment in china. we present here the complete genome sequence of b565 and compare it with 2 published genome sequences of pathogenic strains in aeromonas genus. the result represents an independent step-wise acquisition of virulence factors of pathogenic strains in this genus. | 2011 | 21551296 |
spectroscopic studies on cobalt(ii)-substituted metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | in an effort to probe the structure of a group bb metallo-beta-lactamase, co(ii)-substituted imis was prepared and characterized by electronic absorption, nmr, and epr spectroscopies. imis containing 1 equiv of co(ii) (co(ii)(1)-imis) was shown to be catalytically active. electronic absorption studies of co(ii)(1)-imis revealed the presence of two distinct features: (1) an intense sulfur to co(ii) ligand to metal charge transfer band and (2) less intense, co(ii) ligand field transitions that sug ... | 2005 | 15794654 |
virulence potential and genetic diversity of aeromonas caviae, aeromonas veronii, and aeromonas hydrophila clinical isolates from mexico and spain: a comparative study. | a comparative study of 109 aeromonas clinical isolates belonging to the 3 species most frequently isolated from patients with diarrhea in mexico and spain was performed to investigate the distribution of 3 prominent toxin genes and the gene encoding flagellin of lateral flagella; 4 well-established virulence factors in the genus aeromonas. the aerolysin-hemolysin toxin genes were the most prevalent, being present in 89% of the total isolates. the ast toxin gene was conspicuously absent from the ... | 2007 | 17898843 |
pathogenic potential of a collagenase gene from aeromonas veronii. | the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ... | 2008 | 18388966 |
pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing. | this study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (wwtps) across four countries (china, u.s., canada, and singapore), plus the influent and effluent of one of the 14 wwtps. a total of 370?870 16s rrna gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using rdp classifier and megan. it was found that the most a ... | 2011 | 21780772 |
gastrointestinal colonization rates for human clinical isolates of aeromonas veronii using a mouse model. | a variety of environment-associated gastrointestinal infections have been associated with the aeromonas group of bacteria which contain both non-virulent strains as well as virulent strains within a particular species. this study monitors the colonization rates of colon tissue in a mouse-streptomycin dose/response model involving isolates of aeromonas veronii biovar sobria obtained from human clinical specimens. the ability to successfully colonize mouse colon tissues by the human clinical isola ... | 2011 | 21779939 |
lactobacillus plantarum (vr1) isolated from an ayurvedic medicine (kutajarista) ameliorates in vitro cellular damage caused by aeromonas veronii. | abstract: | 2011 | 21707995 |
differential immune responses of the green neon shrimp (neocaridina denticulate) to dipropyl phthalate. | this study set out to understand the sublethal effect of xenobiotic phthalate esters (paes) on the relationship between the susceptibility of shrimp to bacterial infection and the immune response of the shrimp. neocaridina denticulate were exposed to different concentrations of the pae dipropyl phthalate (dprp), and mortality and six immune parameters were measured on days 1, 3, 5, and 10 after exposure. on days 1 and 3 after exposure, shrimp exposed to 0, 1, 5, 10, and 50 mg/l of dprp and chall ... | 2011 | 21740974 |
different clinical characteristics among aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae monomicrobial bacteremia. | this study aimed to compare the clinical presentations of aeromonas hydrophila, a. veronii biovar sobria and a. caviae monomicrobial bacteremia by a retrospective method at three hospitals in taiwan during an 8-yr period. there were 87 patients with a. hydrophila bacteremia, 45 with a. veronii biovar sobria bacteremia and 22 with a. caviae bacteremia. compared with a. hydrophila and a. veronii biovar sobria bacteremia, a. caviae bacteremia was more healthcare-associated (45 vs 30 and 16%; p = 0. ... | 2011 | 22065896 |
ompa is an adhesion factor of aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract. | in the present study, we focused on one of the aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion. | 2008 | 18828791 |
interaction of aeromonas spp. human isolates with murine macrophages. | this study aimed to evaluate phagocytic and bactericidal activity of macrophages originated from murine j774 macrophage cell line against 26 strains of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila isolated from human fecal specimens. the analysis of phagocytic index (pi) indicated these strains were phagocytosed in low numbers, below 1.1 x l0(-3) for 65% strains of a. veronii biotype sobria and a. hydrophila. the highest pi value (0.019) was noted in murine macroph ... | 2008 | 19123303 |
a chitinase from aeromonas veronii cd3 with the potential to control myxozoan disease. | the class myxosporea encompasses about 2,400 species, most of which are parasites of fish and cause serious damage in aquaculture. due to the concerns about food safety issues and limited knowledge of myxozoa life cycle and fish immune system, no chemicals, antibiotics or immune modulators are available to control myxozoa infection. therefore, little can be done once myxozoa establishment has occurred. | 2011 | 22205999 |
bacterial pathogens and flora isolated from farm-cultured eels (anguilla japonica) and their environmental waters in korean eel farms. | the purpose of this study was to investigate bacterial pathogens and flora in both sick and clinically healthy eels, anguilla japonica, and the environmental rearing waters of korean eel farms. between 2003 and 2010, a total of 621 sick eels were submitted for diagnosis, while 216 healthy eels and 87 environmental water samples were collected during a survey of 26 eel farms in korea. seven different bacterial species were obtained from 183 isolates, which were recovered from the internal organs ... | 2012 | 23357049 |
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram. | sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ... | 2010 | 3116451 |
[dental findings in children with hearing disorders]. | tooth findings are reported of 972 children aged between 3 to 18 years who suffer from impaired hearing. logopedic pupils had significantly more tooth anomalies than those with impaired and normal hearing. the deaf pupils had significantly less dysgnathias than others with impaired hearing. as compared to those with normal hearing there was no significant difference. | 2002 | 124119 |
nitrous oxide and day-case laparoscopy: effects on nausea, vomiting and return to normal activity. | patients admitted for day-case laparoscopy were assigned randomly to receive nitrous oxide-oxygen or oxygen, with enflurane, during a standard anaesthetic technique. postoperative morbidity, in particular nausea and vomiting, and ability to resume normal activity were assessed over the ensuing 48 h. supplementary administration of propofol during the operative procedure was required significantly more often (p less than 0.05) in the absence of nitrous oxide. there was no significant difference i ... | 2010 | 2967712 |
physician compliance in the management of hypertensive patients. | the aim of the present study was to investigate prospectively the value of different forms of intervention in the quality of diagnosis and treatment of essential hypertension in an outpatient clinic. in a first phase (phase 0) 115 patients with a blood pressure of greater than or equal to 140/greater than or equal to 90 mmhg at the initial visit in our outpatient clinic were registered. after a 3 months period the charts of these patients were reviewed. this review revealed that 61 (53%) of thes ... | 2012 | 3870473 |
[the postcholecystectomy syndrome]. | | 2012 | 3454415 |
ronald michael charles welldon. | | 2014 | 4097960 |
[impressions of a study trip to japan]. | | 2015 | 4495220 |
mechanism of action of beta-blockers in hypertension. | | 2001 | 14643 |
pelvic displacement of a cardiac pacemaker following trauma. a case report. | the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope. | 2003 | 152472 |
male transsexualism in england: sixteen cases with surgical intervention. | the syndrome of transsexualism is now regarded as a dinstinct entity separate from transvestism and homosexuality (benjamin, 1966; green and money, 1969). it refers to those patients who since childhood have been convinced of their wrong sex assignment and who wish to attain the sexual characteristics of the opposite sex and to live as a member of that sex. the present report is an initial one referring to the first group of transsexual patients studied at a postgraduate teaching hospital by a m ... | 2005 | 1169047 |
mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels. | mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ... | 2010 | 19900538 |
using computers to take patient histories. | | 2011 | 3147730 |
13c nmr studies of carboxylate inhibitor binding to cobalt(ii) carboxypeptidase a. | both 13c nmr and electronic absorption spectral studies on cobalt(ii) carboxypeptidase a in the presence of acetate and phenylacetate provide evidence for two binding sites for each of these agents. the transverse relaxation rate t2-1 for the 13c-enriched carboxyl groups of the inhibitors is significantly increased when bound to the paramagnetic cobalt carboxypeptidase as compared to the diamagnetic zinc enzyme. the acetate concentration dependence of t2p-1 shows two inflections indicative of se ... | 2012 | 3346659 |
atlanta's homeless find health care. | | 2013 | 3636083 |
[colonoscopic follow up of rectocolonic cancer surgeries. assessment of 64 examinations]. | a collation of 64 colonoscopic examinations performed on 41 patients operated on for cancer of the colon or rectum is reported. eleven patients (26.8 percent) had positive follow-up endoscopic findings. in 13 enumerated tumoral proliferations. 8 benign polyps and 5 malignant growths were found, one of the latter on the anastomosis, and the other 4 on the remaining colon. 80 percent of these tumoral growths and all malignant tumors were discovered in the two years following surgery. when colonic ... | 2014 | 4004135 |
an intrarenal foreign body. | | 2016 | 5041547 |
[development of home care in vorarlberg]. | | 2012 | 3353111 |
three permutations of percutaneous endoscopic gastrostomy. | | 2013 | 3710099 |
eczema vaccinatum. | | 2014 | 4172112 |
are steroids essential for successful maintenance of immunosuppression in heart transplantation? | to determine the necessity for maintenance dosages of prednisone in the management of heart transplant patients, a retrospective study was made of 86 patients undergoing orthotopic heart transplantation and two patients having heart-lung transplantation from june 1985 through october 1986. group 1 (n = 52) had maintenance immunosuppressive therapy that included cyclosporine, azathioprine, and prednisone. group 2 (n = 36) received only cyclosporine and azathioprine with no maintenance dosage of s ... | 2012 | 3316555 |
perforations of the tympanic membrane following the use of goode-type 'long term' tympanostomy tubes. | a retrospective review of 64 goode 't' tube insertions is presented. these were inserted into 61 ears of 48 patients over a period of four years. at the time of review of these 61 ears, 34 tubes were found to be in situ, 27 had extruded spontaneously and three had been removed surgically. ten ears in which the tube had been extruded or removed had central perforations of the tympanic membrane of which eight had been present for more than six months. the number of previous myringotomies or gromme ... | 2011 | 3209952 |
detection and characterization of virulence genes and integrons in aeromonas veronii isolated from catfish. | the presence of virulence genes and integrons was determined in 81 strains of aeromonas veronii isolated from farm-raised catfish. polymerase chain reaction (pcr) protocols were used to determine the presence of genes for cytotoxic enterotoxin (act), aerolysin (aera), two cytotonic enterotoxins (ast, alt), lipase (lip), glycerophospholipid:cholesterol acyltransferase (gcat), serine protease (ser), dnases (exu), elastase (ahyb) and the structural gene flagellin (fla) in the template dna. oligonuc ... | 2010 | 20227596 |
clinical isolates of aeromonas veronii biovar veronii harbor a nonfunctional gene similar to the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus. | thermostable direct hemolysin-related hemolysin encoded by the trh gene is considered a major virulence factor in the pathogenesis of vibrio parahaemolyticus infections. in this study, we report the presence of a trh homolog in three clinical isolates of aeromonas veronii biovar veronii. the presence of a trh homolog in these strains of a. veronii was confirmed by pcr, followed by cloning, sequencing and colony hybridization using a digoxigenin-labelled probe. dna sequence analysis revealed that ... | 2010 | 20636974 |
monitoring of long-term motor activity in depressed patients. | wrist motor activity was continuously recorded by a solid-state monitor in 12 patients with major depression throughout their stay in hospital; their clinical state was evaluated using three scales. during the day, activity troughs and immobility peaks occurred before noon and around 1500 hrs. activity level progressively increased, while the duration of immobility decreased, with clinical improvement. the immobility measurement appeared to be a valuable index of depression and is more sensitive ... | 2013 | 3779292 |
[pharmacotherapy and renal function in the aged]. | | 2015 | 4585245 |
[anatomical contributions of schaffhausen medical school of the 17th century]. | | 2015 | 4569048 |
lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
[experience with ultraviolet irradiation of the blood in chronic diseases]. | | 2016 | 4837149 |
family life cycle resource center in the elementary school. | | 2001 | 98495 |
the chemical use, abuse, and dependence scale (cuad). rationale, reliability, and validity. | this article describes the rationale for the development of the chemical use, abuse, and dependence scale (cuad). the instrument is in a semistructured interview format; it derives both substance use severity scores and dsm-iii-r substance use disorder diagnoses and can be administered in a short period with minimal training. the reliability and validity of the cuad are reported and appear satisfactory. the cuad is recommended for use as a detection, diagnostic, and treatment selection index in ... | 2005 | 1317464 |
[photic maculopathy by low energy laser beam. a case report]. | the authors report a case of photic maculopathy that developed in a 30-year-old laboratory worker after repeated, consecutive exposure to low energy he-ne laser. pigmented foveal change and a small central scotoma in the right eye with window defect on fluorescein angiography characterized this maculopathy. photochemical damage is considered to be the cause of the maculopathy. | 2006 | 1797372 |
[photic maculopathy by low energy laser beam. a case report]. | the authors report a case of photic maculopathy that developed in a 30-year-old laboratory worker after repeated, consecutive exposure to low energy he-ne laser. pigmented foveal change and a small central scotoma in the right eye with window defect on fluorescein angiography characterized this maculopathy. photochemical damage is considered to be the cause of the maculopathy. | 2006 | 1797372 |
mouth opening after release of maxillomandibular fixation in fracture patients. | three hundred eleven patients with mandibular fractures were studied prospectively to ascertain the influence of jaw immobilization on mouth opening. maxillomandibular fixation caused a significant reduction in mouth opening whereas open reduction did not. furthermore, there appeared to be a relationship between the duration of immobilization and the degree of reduction of mouth opening such that the shorter the duration of fixation, the less the reduction. | 2012 | 3471923 |
[indications and technic of proximal selective vagotomy of the gastric acid secretory zone]. | | 2014 | 4283143 |
dental specialties--past, present and future. | | 2015 | 4444815 |
resonance energy transfer in pepsin conjugates. | | 2016 | 4897805 |
[use of optics in the examination and operations for diseases of the pharynx and larynx]. | | 2016 | 4767970 |