| study of the intergenic exef-exeg region and its application as a simple preliminary test for aeromonas spp. | the exef-exeg intergenic regions from different hybridization groups (hg) of aeromonas were studied by pcr amplification using a single pair of primers. six main classes of pcr products were identified according to size: 360 bp, 320 bp, 280 bp, 230-240 bp, 220 bp and 160 bp. direct sequencing of the pcr products indicated that the shorter intergenic regions had probably originated from deletion of dna segments between direct repeats. correlation of certain pcr products with aeromonas caviae (hg4 ... | 1996 | 8935655 |
| characterization of cytotoxic, hemolytic aeromonas caviae clinical isolates and their identification by determining presence of a unique hemolysin gene. | aeromonas caviae has recently been recognized as an important enteropathogen and its hemolysin is purported to be one of the virulence factors, in this study, a total of 80 clinical isolates of aeromonas spp. were investigated by pcr with synthetic oligonucleotides targeting a cloned hemolysin-encoding sequence from an a. caviae isolate of clinical origin. of the 35 clinical a. caviae isolates tested, only 6 contained the target sequence. | 1996 | 8940472 |
| adherence of aeromonas caviae to human cell lines hep-2 and caco-2. | adherence of aeromonas caviae to hep-2 and caco-2 cell monolayers was investigated with 24 clinical isolates. growth phase, temperature, multiplicity of infection and length of incubation affected adherence. treatment of the bacteria with trypsin, sodium metaperiodate, mechanical shearing and the addition of cytochalasin b and cycloheximide to the monolayer significantly reduced the adherence capabilities of the strains investigated. the use of chloramphenicol to inhibit protein synthesis reduce ... | 1996 | 8958248 |
| [new species of aeromonas isolated in cuba]. | one hundred and fifty-five strains of aeromonas isolated in the stools of children under 5 years presenting with acute diarrheal disease were studied. using the aerokey ii system for the identification of species, 47 strains were identified as aeromonas caviae, 58 as aeromonas hydrophila, 23 as aeromonas veronii biovar sobria, 14 as aeromonas trota, 9 as aeromonas veronii biovar veronii, 2 as aeromonas jandaei and 2 as aeromonas shubertii, emphasis is placed on the advantages of this method whic ... | 1995 | 9813480 |
| optimisation of the protocol for detection of aeromonas species in ready-to-eat salads, and its use to speciate isolates and establish their prevalence. | aeromonas spp. are detected in more than 500 cases of gastrointestinal infection each year in england and wales. this study aimed to identify their prevalence in ready-to-eat salads, which are a potential source of aeromonas infection. the protocol for isolation of mesophilic aeromonas spp. from salads was optimised. using the improved method, aeromonas spp were isolated from 19 of 25 samples (25 g) of ready-to-eat salad products. aeromonas organisms were counted, isolates were identified to spe ... | 1998 | 9854886 |
| cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of aeromonas caviae. | a 5.0-kbp ecorv-ecori restriction fragment was cloned and analyzed from genomic dna of aeromonas caviae, a bacterium producing a copolyester of (r)-3-hydroxybutyrate (3hb) and (r)-3-hydroxyhexanoate (3hhx) [p(3hb-co-3hhx)] from alkanoic acids or oils. the nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (pha) synthase gene (phac(ac) [i.e., the phac gene from a. caviae]) together with four open reading frames (orf1, -3, -4, and -5) and one putative promoter region. t ... | 1997 | 9244271 |
| co-expression of polyhydroxyalkanoate synthase and (r)-enoyl-coa hydratase genes of aeromonas caviae establishes copolyester biosynthesis pathway in escherichia coli. | polyhydroxyalkanoate biosynthesis genes of aeromonas caviae were expressed in escherichia coli ls5218 (fadr atoc(con)), and the polyhydroxyalkanoate-producing ability of the recombinants was investigated. a ls5218 strain harboring only phacac (polyhydroxyalkanoate synthase gene) did not accumulate any polyhydroxyalkanoate from dodecanoate in spite of the existence of translated polyhydroxyalkanoate synthase protein, whereas co-expression phacac and phajac ((r)-specific enoyl-coa hydratase gene) ... | 1999 | 9919653 |
| distribution and expression of beta-lactamase genes among aeromonas spp. | clinical and environmental isolates of aeromonas spp. (20 aeromonas caviae, 33 aeromonas veronii and 21 aeromonas hydrophila) were examined for their ability to yield mutants derepressed for beta-lactamase production and for the distribution of the three chromosomally encoded beta-lactamase genes, amps and ceps from a. veronii bv. sobria and cpha from a. hydrophila. of these isolates, 100% and 96% of the isolates from a. hydrophila and a. caviae, respectively, yielded beta-lactamase derepressed ... | 1997 | 9301981 |
| cleavage of influenza a virus h1 hemagglutinin by swine respiratory bacterial proteases. | cleavage of influenza a virus hemagglutinin (ha) is required for expression of fusion activity and virus entry into cells. extracellular proteases are responsible for the proteolytic cleavage activation of avirulent avian and mammalian influenza viruses and contribute to pathogenicity and tissue tropism. the relative contributions of host and microbial proteases to cleavage activation in natural infection remain to be established. we examined 23 respiratory bacterial pathogens and 150 aerobic ba ... | 1997 | 9311838 |
| [post-traumatic endophthalmitis with multiple water pathogens]. | a 37-y-old worker for a recycling company suffered from a corneal perforation by a wire which had been in contact with dirt and moisture. | 1997 | 9333666 |
| enteropathogenic bacteria in the la paz river of bolivia. | diarrheal diseases often result from ingestion of contaminated water or food. the population of la paz, bolivia is directly or indirectly exposed to the sewage-contaminated la paz river. we conducted a bacteriologic survey of the la paz river to quantify the level of bacterial contamination, with particular reference to enteropathogens. a total bacterial count exceeding 10(6) colony-forming units (cfu)/ml, including lactose fermenting and nonfermenting, gram-negative bacilli of approximately 10( ... | 1997 | 9347960 |
| expression and characterization of the recombinant gene encoding chitinase from aeromonas caviae. | the gene encoding a chitinase from aeromonas caviae was cloned by pcr techniques. its recombinant gene expression was performed using pet20b(+) in escherichia coli bl21 (de3). the recombinant chitinase with the extra 33 and 13 amino acids in its n- and c-termini, respectively, was purified to near homogeneity using his-tag affinity chromatography. the recombinant chitinase was found to be present in both the culture medium and the cytoplasm. a single protein band on the native polyacrylamide gel ... | 1997 | 9358657 |
| expression and characterization of (r)-specific enoyl coenzyme a hydratase involved in polyhydroxyalkanoate biosynthesis by aeromonas caviae. | complementation analysis of a polyhydroxyalkanoate (pha)-negative mutant of aeromonas caviae proved that orf3 in the pha locus (a 402-bp gene located downstream of the pha synthase gene) participates in pha biosynthesis on alkanoic acids, and the orf3 gene is here referred to as phaj(ac). escherichia coli bl21(de3) carrying phaj(ac). under the control of the t7 promoter overexpressed enoyl coenzyme a (enoyl-coa) hydratase, which was purified by one-step anion-exchange chromatography. the n-termi ... | 1998 | 9457873 |
| survey for psychrotrophic bacterial pathogens in minimally processed lettuce. | a total of 120 minimally processed, cut and packaged lettuce samples were purchased from retail supermarkets or provided by a salad production facility over an 8-month period. the samples were tested for total aerobic plate counts and for the presence of potentially pathogenic species belonging to the genera of listeria, aeromonas and yersinia. the aerobic plate counts ranged from 103 to 109 colony forming units (cfu) g-1. most samples (76%) contained between 105 and 107 cfu g-1 total aerobic ba ... | 2000 | 10849276 |
| purification and characterization of an aeromonas caviae metalloprotease that is related to the vibrio cholerae hemagglutinin/protease. | a zinc metalloprotease (ap34) from aeromonas caviae was purified by ammonium sulfate precipitation and subsequent gel filtration through sephadex g-100 and sephadex g-50 superfine. the molecular mass was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 34 kda. the protease showed maximum activity at ph 7.0 and was stable at 60 degrees c. ap34 was completely inactivated by edta and zincov. the n-terminal amino acid sequence of ap34 showed a high degree of homology with ... | 1999 | 9919673 |
| antimicrobial resistance of mesophilic aeromonas spp. isolated from two european rivers. | the activity of 19 antibiotics and four antiseptics and/or disinfectants was studied against 138 non-redundant strains of aeromonas spp. (104 aeromonas caviae, 22 aeromonas sobria and 12 aeromonas hydrophila) isolated from two european rivers. antibiotic resistance frequencies were: nalidixic acid, 59%; tetracycline, 14%; fosfomycin, 8%; tobramycin and cotrimoxazole, 7%; cefotaxime, 4%; chloramphenicol, 2%; gentamicin, 1%. most of the nalidixic acid-resistant strains were susceptible to fluoroqu ... | 2000 | 10933657 |
| enumeration and confirmation of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria isolated from raw milk and other milk products in northern greece. | a total of 138 raw cow's and 57 raw ewe's milk samples; 80 pasteurized cow's milk samples; 39 anthotyros cheese, 36 manouri cheese, and 23 feta cheese samples; and 15 rice pudding samples were examined for the presence and any countable population of aeromonas species. twenty-two (15.9%) of the 138 cow's milk samples analyzed were contaminated with a. hydrophila. in 13 of these samples, populations of 3.0x10(2) to 5.0x10(3) cfu/ml were counted in starch ampicillin agar (saa). eighteen cow's milk ... | 1999 | 10340665 |
| intramolecular chaperone and inhibitor activities of a propeptide from a bacterial zinc aminopeptidase. | an aminopeptidase from aeromonas caviae t-64 was translated as a preproprotein consisting of three domains; a signal peptide (19 amino acid residues), an n-terminal propeptide (101 residues) and a mature region (273 residues). we demonstrated that a proteinase, which was isolated from the culture filtrate of a. caviae t-64, activated the recombinant pro-aminopeptidase by removal of the majority of the propeptide. using l-leu-p-nitroanilide as a substrate, the processed aminopeptidase showed a la ... | 1999 | 10377241 |
| virulence factors in aeromonas spp and their association with gastrointestinal disease. | culture filtrates of eight aeromonas strains isolated from the feces of 487 subjects (292 diarrhoeic patients and 195 asymptomatic subjects) were tested for toxin production in cho and mccoy cells, and adhesion and invasive ability in caco-2 cells. among these isolates, three aeromonas sobria and one aeromonas caviae strains possessed virulence-associated properties. toxin production was the most common of the three virulence properties. two a. caviae were associated, in the absence of other dia ... | 1998 | 9497926 |
| biosynthesis of polyhydroxyalkanoates (pha) by recombinant ralstonia eutropha and effects of pha synthase activity on in vivo pha biosynthesis. | recombinant strains of ralstonia eutropha phb 4, which harbored aeromonas caviae polyhydroxyalkanoates (pha) biosynthesis genes under the control of a promoter for r. eutropha phb operon, were examined for pha production from various alkanoic acids. the recombinants produced poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] from hexanoate and octanoate, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxypentano ate) [p(3hb-co-3hv-co-3hhp)] from pentanoate and nonanoate. one ... | 1999 | 10416652 |
| expression of aeromonas caviae bla genes in escherichia coli. | an isolate of aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. transfer of the plasmid to escherichia coli k12 resulted in the expression of a tem-like beta-lactamase that was not expressed in parental a. caviae. the bla gene sequence was detectable by dna hybridization and pcr amplification of the plasmid when extracted from parental a. caviae or from e. coli k12 transconjugants. sequence analysis of the bla genes revealed in each case an 858 bp open reading frame, differing fr ... | 1996 | 8889718 |
| analysis of the interaction of aeromonas caviae, a. hydrophila and a. sobria with mucins. | aeromonas species are known to be involved in human gastrointestinal diseases. these organisms colonize the gastrointestinal tract. aeromonas hydrophila, a. caviae, and a. sobria have been demonstrated microscopically to adhere to animal cell lines that express mucous receptors, but quantitative studies of adherence to mucosal components such as mucin have not been published to date. purified bovine submaxillary gland, hog gastric mucin, and fish skin mucin were used as a model to study mucin-bi ... | 1998 | 9566493 |
| role of flm locus in mesophilic aeromonas species adherence. | the adherence mechanism of aeromonas caviae sch3n to hep-2 cells was initially investigated through four mini-tn5 mutants that showed a 10-fold decrease in adherence. these mutants lost motility, flagella, and their lipopolysaccharide (lps) o antigen (o-ag). three genes, flmb-neua-flmd, were found to be interrupted by the transposon insertions; additionally, two other genes, one lying upstream (flma) and one downstream (neub), were found to be clustered in the same operon. while the flma and flm ... | 2001 | 11119490 |
| co-expression of 3-ketoacyl-acp reductase and polyhydroxyalkanoate synthase genes induces pha production in escherichia coli hb101 strain. | the escherichia coli 3-ketoacyl-acp reductase gene (fabgec) was cloned using a pcr technique to investigate the metabolic link between fatty acid metabolism and polyhydroxyalkanoate (pha) production. three plasmids respectively harboring fabgec and the poly-3-hydroxyalkanoate synthesis genes phacac and phac1ps from aeromonas caviae and pseudomonas sp. 61-3 respectively were constructed and introduced into e. coli hb101 strain. on a two-stage cultivation using dodecanoate as the sole carbon sourc ... | 1999 | 10418145 |
| phenotypic identification of aeromonas genomospecies from clinical and environmental sources. | a collection of 983 aeromonas isolates from environmental and clinical sources have been identified to the genomospecies level. a phenotypic method identified 93% of the strains. the use of citrate and the production of acid from sorbitol enabled the members of the aeromonas hydrophila complex to be separated. the most common genomospecies from intestinal sources were aeromonas veronii biotype sobria and aeromonas caviae. the former, together with a. hydrophila, was the most frequently isolated ... | 1998 | 9575026 |
| characterization of aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in bangladesh. | aeromonas isolates from patients with diarrhea in bangladesh (n = 69), from healthy controls (n = 11), and from surface water (n = 40) were analyzed with respect to their hybridization groups (hgs) by the aid of fatty acid methyl ester (fame) characterization and dna fingerprinting by aflp, biochemical phenotypes (phe-neplate [php] types), and the production of hemolysin and cytotoxin. the aim of the investigation was to find out whether certain strains carrying virulence factors predominated am ... | 1997 | 9003598 |
| efficient production of polyhydroxyalkanoates from plant oils by alcaligenes eutrophus and its recombinant strain. | the ability of alcaligenes eutrophus to grow and produce polyhydroxyalkanoates (pha) on plant oils was evaluated. when olive oil, corn oil, or palm oil was fed as a sole carbon source, the wild-type strain of a. eutrophus grew well and accumulated poly(3-hydroxybutyrate) homopolymer up to approximately 80% (w/w) of the cell dry weight during its stationary growth phase. in addition, a recombinant strain of a. eutrophus phb-4 (a pha-negative mutant), harboring a pha synthase gene from aeromonas c ... | 1998 | 9581296 |
| [camp factor for the differentiation among aeromonas species]. | the camp factor technique, described by natale figura as a presumptive method for the differentiation of the species hydrophila, sobria and caviae, was applied to 80 aeromonas strains isolated from children under 5 years with acute diarrheic disease. the typical phenomenon was seen in aerobiosis and anaerobiosis conditions in 10 strains, which were classified as aeromonas hydrophila. in aerobiosis conditions/alone, it was observed in 20 strains, which were identified as aeromonas sobria. it was ... | 1992 | 9768204 |
| [phenotype markers in strains of aeromonas isolated in cuba from children with an acute diarrheic disease]. | twenty-seven cases of aeromonas isolated from 300 five-years children with acute diarrheal disease (eda) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. the percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher th ... | 1995 | 9805080 |
| behavior of listeria monocytogenes and aeromonas spp. on fresh-cut produce packaged under equilibrium-modified atmosphere. | storage experiments were conducted to follow the behavior of pathogens on fresh-cut vegetables (trimmed brussels sprouts, grated carrots, shredded iceberg lettuce, and shredded chicory endives) packaged under an equilibrium-modified atmosphere (ema) (2 to 3% o2, 2 to 3% co2, and 94 to 96% n2) and stored at 7 degrees c. as a comparison, fresh-cut vegetables were also packaged in a perforated high-barrier film (air conditions) and stored at 7 degrees c. in a first step, the shelf life of the veget ... | 1999 | 10528715 |
| [case of infectious hepatic cyst caused by aeromonas caviae]. | | 1999 | 10581795 |
| hemolytic activity and siderophore production in different aeromonas species isolated from fish. | the hemolytic activity and siderophore production of several strains of motile aeromonads were determined. the hemolytic activity of aeromonas caviae and aeromonas eucrenophila was enhanced after trypsinization of the samples. the enhancement of hemolysis was observed in strains that carried an aerolysin-like gene, detected by a pcr procedure. siderophore production was demonstrated in all but one strain of aeromonas jandaei. no apparent relationship was observed between the presence of plasmid ... | 1999 | 10584028 |
| aeromonas-associated diarrhoea in bangladeshi children: clinical and epidemiological characteristics. | we studied the clinical and epidemiological features associated with aeromonas diarrhoea by a hospital survey of 7,398 children under 5 years of age presenting with diarrhoea. the data were actually based upon two cohorts from this survey, the majority of the data being identified from 405 (5.5%) in whom aeromonas was the sole enteric pathogen. aeromonas caviae was the most prevalent species, accounting for 32% (129/405) of all isolates. eighty-three per cent of children with aeromonas-associate ... | 1999 | 10605516 |
| impact of an urban effluent on antibiotic resistance of riverine enterobacteriaceae and aeromonas spp. | in order to evaluate the impact of an urban effluent on antibiotic resistance of freshwater bacterial populations, water samples were collected from the arga river (spain), upstream and downstream from the wastewater discharge of the city of pamplona. strains of enterobacteriaceae (representative of the human and animal commensal flora) (110 isolates) and aeromonas (typically waterborne bacteria) (118 isolates) were selected for antibiotic susceptibility testing. most of the aeromonas strains (7 ... | 2000 | 10618213 |
| detection, prevalence, purification and characterization of lecithinase of klebsiella pneumoniae. | lecithinase activity in klebsiella is a rare trait as out of 208 strains of klebsiella belonging to 3 species, viz. k. pneumoniae (168), k. planticola (29) and k. oxytoca (11), only 4 strains of k. pneumoniae produced lecithinase positive colonies on egg-yolk-agar. although cell lysates of 16 k. pneumoniae yielded positive results for lecithinase assay on egg-yolk-agar, 19 strains were detected positive for lecithinase with elisa using anti-lecithinase serum. release of up to 52.12% cell-bound l ... | 1999 | 10687289 |
| molecular cloning of two (r)-specific enoyl-coa hydratase genes from pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis. | two pseudomonas aeruginosa genes, termed phaj1(pa) and phaj2(pa), homologous to the aeromonas caviae (r)-specific enoyl-coa hydratase gene (phaj(ac)) were cloned using a pcr technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (pha) synthesis from beta-oxidation cycle. two expression plasmids for phaj1(pa) and phaj2(pa) were constructed and introduced into escherichia coli dh5alpha strain. the recombinants harboring phaj1(pa) or phaj2(pa) showed high (r)-specific enoyl ... | 2000 | 10713420 |
| purification and some properties of high-molecular-weight xylanases, the xylanases 4 and 5 of aeromonas caviae w-61. | aeromonas caviae w-61 produces multiple extracellular xylanases, the xylanases 1, 2, 3, 4, and 5 [nguyen, v. d. et al., biosci. biotechnol. biochem., 56, 1708-1712 (1993)]. here we purified and characterized high-molecular-weight xylanases, the xylanases 4 and 5 from the culture fluids of the bacterium. the purified xylanases 4 and 5, which had molecular masses of 120 and 140 kda, respectively, were endo-beta-1,4-xylanases with similar enzymatic properties except for trans-xylosidase activity. t ... | 2000 | 10737201 |
| site-directed mutagenesis of asp313, glu315, and asp391 residues in chitinase of aeromonas caviae. | site-directed mutagenesis was used to explore the roles of amino acid residues involved in the activity of chitinase from aeromonas caviae. kinetic parameters for 4-methylumbelliferyl-n,n'-diacetyl-chitobiose or 4-methylumbelliferyl-n,n',n"-triacetylchitotriose hydrolysis were determined with wild-type and mutant chitinases. chitinases with the mutations e315d (or q) and d391e (or n) were severely impaired and had dramatically decreased kcat. however, the effect of the these mutations on the km ... | 1999 | 10794597 |
| cloning, sequencing and expression of the gene encoding the extracellular metalloprotease of aeromonas caviae. | a gene (apk) encoding the extracellular protease of aeromonas caviae ae6 has been cloned and sequenced. for cloning the gene, the dna genomic library was screened using skim milk lb agar. one clone harboring plasmid pkk3 was selected for sequencing. nucleotide sequencing of the 3.5 kb region of pkk3 revealed a single open reading frame (orf) of 1,785 bp encoding 595 amino acids. the deduced polypeptide contained a putative 16-amino acid signal peptide followed by a large propeptide. the n-termin ... | 2000 | 10888351 |
| inhibition of aeromonas caviae and a. sobria by sodium choloride, citric acid, ascorbic acid, potassium sorbate and extracts of thymus vulgaris. | the respective and combined effects of sodium chloride, ascorbic acid, citric acid, potassium sorbate, and thymus vulgaris extract on the growth of aeromonas caviae and aeromonas sobria were investigated. sodium chloride (3%) significantly reduced the growth and 4% nacl inhibited growth of the tested strains. ascorbic acid (0. 1%), potassium sorbate (0.05%), and citric acid (0.03%) slightly inhibited growth. t. vulgaris extract (0.3%) greatly reduced the growth. various combinations of these com ... | 2000 | 10957708 |
| [cultivation optimization of prolyl endopeptidase and high cell density fermentation]. | engineered e. coli bl21/pgem-pep could constitutively express recombinant prolyl endopeptidase from aeromonas punctata, which was extremely effected by culture conditions, so fermentation conditions were optimized to obtain it's high expression level. firstly, the stability of bl21/pgem-pep was investigated, then, culture temperature, ph, time, medium were optimized in shake flaskd. the l9(3(4)) orthogonal experiment confirmed that shaker speed, ph, culture temperature, culture time had high deg ... | 2000 | 10976323 |
| epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping. | ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ... | 2000 | 10997832 |
| use of a chia probe for detection of chitinase genes in bacteria from the chesapeake bay(1). | pcr primers specific for the chia gene were designed by alignment and selection of highly conserved regions of chia sequences from serratia marcescens, alteromonas sp., bacillus circulans and aeromonas caviae. these primers were used to amplify a 225 bp fragment of the chia gene from vibrio harveyi to produce a chia gene probe. the chia pcr primers and probe were used to detect the presence of the chia gene in an assemblage of 53 reference strains and gave consistent results. selected chia fragm ... | 2000 | 11053737 |
| virulence factors of clinical isolates of aeromonas caviae. | the mechanism of pathogenicity of 13 aeromonas caviae strains isolated from fecal specimens of children with diarrhea was examined. isolates possessed several virulence agents. six of 13 isolates produced cholera-like toxin, all strains exhibited adhesive ability and secreted catecholate siderophores. six isolates were able to grow in heat-inactivated serum. production of hemolysins was not detected. the isolates had relatively high values of ld50 in a mouse model, which suggests that there is n ... | 2001 | 11830944 |
| [purification and characterization of recombinant aeromonas punctata prolyl endopeptidase]. | the study of down-stream techniques of recombinant aeromonas punctata prolyl endopeptidase (appep) was presented here. high cell-density fermentation of e. coli bl21/pkkh-pep in nbs bioflo 3000 5 l fermentor was achieved, the final cell density was 22.5 g (dcw)/l after 14 h cultivation, the yield of appep expressed in soluble protein was 3.0 g per litter broth. after sonication, the supernatant of free cell extract was purified by ammonium sulfate fractionation, high performance q sepharose ff, ... | 2000 | 11059278 |
| effect of sodium chloride and citric acid on growth and toxin production by a. caviae and a. sobria at moderate and low temperatures. | the effect of sodium chloride and citric acid on hemolysin and caseinase production by aeromonas caviae and aeromonas sobria at 32 degrees c and 5 degrees c was investigated. at 32 degrees c, although both strains were tolerant to 3% nacl in tsb, the production of caseinase was decreased in the presence of 1-3% nacl, and the production of hemolysin was abolished by 2-3% nacl. citric acid (0.03%) was less effective than nacl in reducing hemolysin and caseinase production by both strains at 32 deg ... | 2000 | 11061632 |
| prevalence and molecular characterization of aeromonas spp. in ready-to-eat foods in italy. | a survey was carried out in italy to ascertain the prevalence of aeromonas spp. in ready-to-eat foods (vegetables, cheeses, meat products, and ice creams) and the level of molecular heterogeneity of the isolates found by macrorestriction analysis of genomic dna with pulsed-field gel electrophoresis (pfge). in total, 46 (14.4%) of the 320 food samples examined were found positive for aeromonas spp. the highest percentages of isolation were discovered in vegetables, particularly lettuce (45.0%), e ... | 2000 | 11131903 |
| crystallization and preliminary x-ray analysis of (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis. | dimeric (r)-specific enoyl-coenzyme a (coa) hydratase from aeromonas caviae catalyzes the hydration of trans-2-enoyl-coas with carbon lengths of 4-6 to yield their corresponding (r)-3-hydroxyacyl-coas and is essential for polyhydroxyalkanoate (pha) biosynthesis. the enzyme has been crystallized by vapour diffusion against a reservoir solution containing 20% polyethylene glycol 4000, 5% 2-propanol and 20 mm hepes ph 7.0 at 298 k. crystals belong to the monoclinic space group c2, with unit-cell pa ... | 2001 | 11134939 |
| lateral flagella of aeromonas species are essential for epithelial cell adherence and biofilm formation. | mesophilic aeromonas strains express a single polar flagellum in all culture conditions and produce lateral flagella on solid media. such hyperflagellated cells demonstrate increased adherence. nine lateral flagella genes, lafa-u for aeromonas hydrophila, and four aeromonas caviae genes, lafa1, lafa2, lafb and fliu, were isolated. mutant characterization, nucleotide and n-terminal sequencing demonstrated that the a. hydrophila and a. caviae lateral flagellins were almost identical, but were dist ... | 2002 | 11985716 |
| aeromonas caviae as a cause of cholecystitis. | | 2000 | 11217277 |
| molecular cloning and expression in escherichia coli of the extracellular endoprotease of aeromonas caviae t-64, a pro-aminopeptidase processing enzyme(1). | pa protease (pro-aminopeptidase processing protease) activates the pro-aminopeptidases from aeromonas caviae t-64 and vibrio proteolytica by removal of their pro-regions. cloning and sequencing of the pa protease gene revealed that pa protease was translated as a preproprotein consisting of four domains: a signal peptide; an n-terminal propeptide; a mature region; and a c-terminal propeptide. the deduced amino acid sequence of the pa protease precursor showed significant homology with several ba ... | 1999 | 10446382 |
| xynx, a possible exo-xylanase of aeromonas caviae me-1 that produces exclusively xylobiose and xylotetraose from xylan. | a gene, xynx, encoding a novel xylanase, was cloned from aeromonas caviae me-1. this gene encoded an enzyme that was constituted of 334 amino acid residues (38,580 da) and was similar in sequence to family 10 (family f) beta-1,4 endo-xylanases. xynx produced only xylobiose and xylotetraose from birch wood xylan, and xylotriose, xylopentaose, and higher oligosaccharides were not detected in the tlc analysis. we designated it as x2/x4-forming xylanase. this enzyme does not have transglycosylation ... | 1999 | 10500996 |
| enhancement of the virulence of aeromonas caviae diarrhoeal strains by serial passages in mice. | thirteen clinical aeromonas caviae isolates from the faeces of 13 children with mild to severe diarrhoea were tested for enhancement of mouse lethality, adhesion ability, siderophore and cholera toxin cross-reactive (ctc) factor production, by four consecutive passages through mice by intraperitoneal injection of a. caviae suspensions. the passaged a. caviae strains were re-isolated from monomicrobic cardiac blood samples and inocula were prepared for the next passage. all a. caviae isolates pos ... | 2001 | 11289515 |
| mesophilic aeromonads in wild and aquacultured freshwater fish. | numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ... | 2001 | 11348001 |
| g561 site-directed deletion mutant chitinase from aeromonas caviae is active without its 304 c-terminal amino acid residues. | a g561 mutant of the aeromonas caviae chitinase chia was made by pcr site-directed deletion mutagenesis in order to study the role of the 304 c-terminal amino acid residues of chia in the enzymatic hydrolysis of chitin. the recombinant chiag561 encoded on a 1.6-kb dna fragment of a. caviae chia was expressed in a heterologous escherichia coli host using the pet20b(+) expression system. the his-tag-affinity-purified recombinant chiag561 had a calculated molecular mass of 63,595 da, which was cons ... | 2001 | 11357514 |
| motility and the polar flagellum are required for aeromonas caviae adherence to hep-2 cells. | aeromonas caviae is increasingly being recognized as a cause of gastroenteritis, especially among the young. the adherence of aeromonads to human epithelial cells in vitro has been correlated with enteropathogenicity, but the mechanism is far from well understood. initial investigations demonstrated that adherence of a. caviae to hep-2 cells was significantly reduced by either pretreating bacterial cells with an antipolar flagellin antibody or by pretreating hep-2 cells with partially purified f ... | 2001 | 11401962 |
| the role of the fatty acid beta-oxidation multienzyme complex from pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadba operon from p. oleovorans and of the enoyl-coa hydratase genes phaj from p. oleovorans and pseudomonas putida. | in order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (fadba) in the provision of (r)-3-hydroxyacyl-coa thioesters for medium-chain-length polyhydroxyalkanoate (pha(mcl)) biosynthesis, the fadba(po) operon of pseudomonas oleovorans was cloned and characterized. the fadba(po) operon and a class-ii pha synthase gene of pseudomonas aeruginosa were heterologously co-expressed in escherichia coli to determine whether the putative epimerase funct ... | 2002 | 12115060 |
| comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in belgium. | the aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxytetracycline-resistant (ot(r)) heterotrophic bacterial population in two belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. the study of ot(r) heterotrophs and mesophilic aeromonas spp. allowed comparison of tolerance data at the intergenus as well as at the intragenus level. in total, 354 independently obtained ot(r) is ... | 2001 | 11403391 |
| virulence factors in vibrios and aeromonads isolated from seafood. | thirty-one isolates from seafood, identified as aeromonas hydrophila (7), aeromonas caviae (11), vibrio parahaemolyticus (3), vibrio fluvialis (5), vibrio alginolytictus (3), vibrio metschnikovii (1) and vibrio damsela (1), were tested for possible virulence factors including extracellular hydrolytic enzymes, haemolysins, cytotoxins (vero and hep-2 cells) and adherence ability (hep-2 cells). all the a. hydrophila strains were beta-haemolytic and produced cytotoxins as well as one strain of v. fl ... | 2001 | 11497085 |
| biochemical identification and numerical taxonomy of aeromonas spp. isolated from environmental and clinical samples in spain. | to study the phenotypic characteristics of aeromonas spp. from environmental and clinical samples in spain and to cluster these strains by numerical taxonomy. | 2002 | 12174040 |
| a secreted aminopeptidase of pseudomonas aeruginosa. identification, primary structure, and relationship to other aminopeptidases. | using leucine-p-nitroanilide (leu-pna) as a substrate, we demonstrated aminopeptidase activity in the culture filtrates of several pseudomonas aeruginosa strains. the aminopeptidase was partially purified by deae-cellulose chromatography and found to be heat stable. the apparent molecular mass of the enzyme was approximately 56 kda; hence, it was designated ap(56). heating (70 degrees c) of the partially purified aminopeptidase preparations led to the conversion of ap(56) to a approximately 28-k ... | 2001 | 11533066 |
| helicobacter pylori and other enteric bacteria in freshwater environments in mexico city. | helicobacter pylori infection is common in the mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear. | 2001 | 11578764 |
| characterization of 13 kda granule-associated protein in aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria. | analysis of native poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] inclusions from aeromonas caviae fa440 revealed that orf1 (a 348-bp gene located immediately upstream of phac(ac)) encodes a 13-kda granule-associated protein, which was referred to as phap(ac). several recombinant strains of a. caviae were constructed and conducted to analyze their pha-producing abilities. a transconjugant of fa440 harboring additional copies of phapcj(ac) genes accumulated p(3hb-co-3hhx) copolyes ... | 2001 | 11749166 |
| lateral flagella and swarming motility in aeromonas species. | swarming motility, a flagellum-dependent behavior that allows bacteria to move over solid surfaces, has been implicated in biofilm formation and bacterial virulence. in this study, light and electron microscopic analyses and genetic and functional investigations have shown that at least 50% of aeromonas isolates from the species most commonly associated with diarrheal illness produce lateral flagella which mediate swarming motility. aeromonas lateral flagella were optimally produced when bacteri ... | 2002 | 11751834 |
| isolation and identification of motile aeromonas species from chicken. | in this study a total of 140 broiler carcasses and carcass parts purchased at different supermarkets in ankara including 50 whole carcass, 30 wing, 30 leg and 30 breast samples were analysed for the presence of motile aeromonas species. according to analysis findings, motile aeromonas spp. were isolated from 116 (82.9%) of total 140 samples. the distribution of the isolates were 94%, 86.6%, 80%, 63.3% in broiler carcass, wing, leg and breast samples, respectively. aeromonas hydrophila was isolat ... | 2001 | 11765602 |
| effect of high oxygen modified atmosphere packaging on microbial growth and sensorial qualities of fresh-cut produce. | the application of high oxygen atmospheres (hoa) (i.e. > 70% o2) for packaging ready-to-eat vegetables was evaluated as an alternative technique for low o2 equilibrium modified atmosphere (ema) packaging (3% o2-5% co2-balance n2) for respiring products. comparative experiments between both techniques were performed in-vitro and in-vivo. typical spoilage causing microorganisms (pseudomonas fluorescens, candida lambica), the moulds botrytis cinerea, aspergillus flavus and the opportunistic psychro ... | 2001 | 11789938 |
| type ii topoisomerase quinolone resistance-determining regions of aeromonas caviae, a. hydrophila, and a. sobria complexes and mutations associated with quinolone resistance. | most aeromonas strains isolated from two european rivers were previously found to be resistant to nalidixic acid. in order to elucidate the mechanism of this resistance, 20 strains of aeromonas caviae (n = 10), a. hydrophila (n = 5), and a. sobria (n = 5) complexes, including 3 reference strains and 17 environmental isolates, were investigated. fragments of the gyra, gyrb, parc, and pare genes encompassing the quinolone resistance-determining regions (qrdrs) were amplified by pcr and sequenced. ... | 2002 | 11796341 |
| detection of aeromonas caviae in the common housefly musca domestica by culture and polymerase chain reaction. | aeromonas caviae has been implicated in diarrhoeal disease of livestock and humans. the potential role of houseflies in the epidemiology of this pathogen was investigated by examining the prevalence of a. caviae in houseflies collected from two south carolina farms and one restaurant. isolation was accomplished by culture of flies in alkaline peptone water followed by identification with aeromonas-specific pcr using novel primers (apw-pcr). all isolates cultured from houseflies were identified a ... | 2001 | 11811891 |
| influence of polarisation and differentiation on interaction of 43-kda outer-membrane protein of aeromonas caviae with human enterocyte-like caco-2 cell line. | it has been recognised that adherence and invasion to host cells are important steps in the pathogenesis of entero-pathogenic bacteria, including aeromonas caviae. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interaction of a. caviae isolates to caco-2 cells in different polarisation and differentiation conditions. the adherence of a. caviae may be related to accessibility of host cell basolateral receptors. aggregative a. caviae is ... | 2003 | 12684708 |
| in vitro antimicrobial susceptibility of clinical isolates of aeromonas caviae, aeromonas hydrophila and aeromonas veronii biotype sobria. | | 2002 | 11909850 |
| predictive modelling for packaging design: equilibrium modified atmosphere packages of fresh-cut vegetables subjected to a simulated distribution chain. | the impact of temperature fluctuations in a simulated cold distribution chain, typical of commercial practice, was investigated on both the microbial and sensorial quality of equilibrium modified atmosphere (ema) packaged minimally processed vegetables. the internal o2 concentration of the designed packages could be predicted for the different steps of the simulated distribution chain by applying an integrated mathematical system. the internal atmosphere in the packages remained in its aerobic r ... | 2002 | 11934040 |
| sequence analysis and biochemical characterisation of chromosomal cav-1 (aeromonas caviae), the parental cephalosporinase of plasmid-mediated ampc 'fox' cluster. | aeromonas caviae cip 74.32 was resistant to amoxicillin, ticarcillin and cephalothin, and susceptible to cefoxitin, cefotaxime, ceftazidime, aztreonam and imipenem. this strain produced a cephalosporinase (pi 7.2) and an oxacillinase (pi 8.5). the cephalosporinase gene cav-1 was cloned and sequenced. unlike a. caviae donor, escherichia coli pnce50 transformant producing cav-1 beta-lactamase was resistant to cefoxitin. the deduced protein sequence cav-1 contained 382 amino acids, and shared >96% ... | 2003 | 12757951 |
| enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (pha) copolyester by in vitro evolution of aeromonas caviae pha synthase. | by in vitro evolution experiment, we have first succeeded in acquiring higher active mutants of a synthase that is a key enzyme essential for bacterial synthesis of biodegradable polyester, polyhydroxyalkanoate (pha). aeromonas caviae fa440 synthase, termed phac(ac), was chosen as a good target for evolution, since it can synthesize a pha random copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate [p(3hb-co-3hhx)] that is a tough and flexible material compared to polyhydroxybutyrate (phb) hom ... | 2002 | 11976116 |
| in vitro stepwise autoprocessing of the proform of pro-aminopeptidase processing protease from aeromonas caviae t-64. | pa protease (pro-aminopeptidase processing protease) is an extracellular zinc metalloprotease produced by the gram-negative bacterium aeromonas caviae t-64. the 590-amino-acid precursor of pa protease is composed of a putative 19-amino-acid signal sequence, a 165-amino-acid n-terminal propeptide, a 33 kda mature protease domain and an 11 kda c-terminal propeptide. the proform of pa protease, which was produced as inclusion bodies in escherichia coli, was subjected to in vitro refolding. it was r ... | 2002 | 11983417 |
| aeromonas veronii biovar veronii septicaemia and acute suppurative cholangitis in a patient with hepatitis b. | gram-negative bacilli of the genus aeromonas are widespread in aquatic environments and can be responsible for human infections. although aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, aeromonas septicaemia remains an uncommon finding, often associated with serious underlying disease and predominantly related to the species aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae. here, a case of a. veronii biovar ve ... | 2003 | 12867570 |
| engineering of ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from fructose and solid-state properties of the copolymer. | recombinant ralstonia eutropha capable of producing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer [p(3hb-co-3hhx)] from fructose was engineered by introduction of genes for crotonyl-coa reductase (ccr) from streptomyces cinnamonensis (ccrsc) and for pha synthase and (r)-specific enoyl-coa hydratase from aeromonas caviae (phac-jac). in this recombinant strain, c6-acyl-coa intermediates were provided via beta-ketothiolase-mediated elongation of butyryl-coa, which was generated from croto ... | 2002 | 12005535 |
| [variability of pseudomonas punctata]. | | 1953 | 13081082 |
| synthesis of a novel class of polyhydroxyalkanoates in arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation. | the poly[(r)-3-hydroxyalkanoate] (pha) synthase gene (phac(ac)) of aeromonas caviae fa440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. the modified gene was introduced into arabidopsis thaliana plants by agrobacterium-mediated transformation. the transgenic arabidopsis plant expressed the introduced gene and its protein, and it accumulated pha in its tissues. gas chromatography-mass spectrometry analy ... | 2002 | 12040103 |
| studies of aeromonas formicans crawford comb. nov. from soluble oil emulsions. | | 1957 | 13416178 |
| taxonomic relationships among the pseudomonads. | colwell, r. r. (university of washington, seattle), and j. liston. taxonomic relationships among the pseudomonads. j. bacteriol. 82:1-14. 1961.-an electronic computer technique, utilizing the adansonian principle that every feature should have equal weight, was applied in an effort to derive a taxonomy of the pseudomonas-achromobacter group of gram-negative, asporogenous, rodlike bacteria. the validity of the general method was tested by an analysis of 40 well defined strains, principally derive ... | 1961 | 13694873 |
| taxonomic analysis with the electronic computer of some xanthomonas and pseudomonas species. | colwell, r. r. (university of washington, seattle) and j. liston. taxonomic analysis with the electronic computer of some xanthomonas and pseudomonas species. j. bacteriol. 82:913-919. 1961.-forty organisms, including 16 xanthomonas spp., 5 strains of pseudomonas solanacearum, 4 strains of p. aeruginosa, 6 strains of p. fluorescens, 5 freshly isolated strains of pseudomonads, as well as the american type culture collection strains of flavobacterium aquatile, vibrio cuneatus, vibrio metschnikovii ... | 1961 | 13880721 |
| an expression release system for heterogeneous proteins in e.coli mediated by the kil gene. | an expression release system for heterogeneous proteins in escherichia coli mediated by the colicin release gene(kil gene) was constructed. the system is based on the ability of the kil protein to release periplasmic proteins into the growth medium. beta-lactamase, an e.coli periplasmic protein, and prolyl endopeptidase (pep), a periplasmic protein of aeromonas punctata subsp. punctata were used as report proteins. commonly, these two proteins are seldom released into the growth medium. the resu ... | 2001 | 12040395 |
| identification of a 43-kda outer-membrane protein as an adhesin in aeromonas caviae. | aeromonas spp. are associated with intestinal and extra-intestinal infections. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interactions involved in the adherence of a. caviae isolates ae56, ae391 and ae398 to hep-2 cells. all strains expressed high levels of aggregative adherence. maximum adhesion occurred with bacteria grown at 22 degrees c, but transmission electron microscopy did not reveal the presence of fimbrial structures on ... | 2001 | 11289516 |
| characterization of the pro-aminopeptidase from aeromonas caviae t-64. | the pro-aminopeptidase from aeromonas caviae t-64 (pro-apac) had maximal activity at 60 degrees c and was more stable than mature apac at temperature up to 65 degrees c for 1 hour. the ph stability of pro-apac ranged from 4.0 to 8.0, which is broader than the range for the mature apac. the kcat/km of pro-apac was 1.4% to 24% of that of mature apac. | 2001 | 11302179 |
| cloning, expression, and characterization of a family 52 beta-xylosidase gene (xysb) of a multiple-xylanase-producing bacterium, aeromonas caviae me-1. | a lambda phage genomic library of aeromonas caviae me-1, a multiple-xylanase-producing bacterium, was screened for xylan degradation activities. we isolated one clone, b65, which had weak xylanase activity, by the dns method, but gave no visible bands on zymogram assay using sds-xylan-page. based on tlc analyses of enzymatic products and some glycosidase assays using p-nitrophenyl substrates, we established that pb65 encodes a beta-xylosidase gene. in the nucleotide sequence analysis, we found a ... | 2001 | 11330658 |
| properties of recombinant aeromonas punctata prolyl endopeptidase. | the properties of recombinant aeromonas punctata prolyl endopeptidase(appep) were studied using specific substrate and peptides. results show that the optimum catalytic temperature and ph was 34 degrees and 8.4, the stability of the appep was in the range of 4-32 degrees and ph 6.0-10.0, and its k(m) was 0.03 mmol/l based on the z-gly-pro-betana. the appep was not sensitive to pmsf, tlck, tpck, trypsion inhibitor, edta, tetrathionate and some metal ions, but was sensitive to sds and zn(2 ), and ... | 1999 | 12110936 |
| enteropathogenic activity and invasion of hep-2 cells by aeromonas caviae clinical isolates. | twenty aeromons caviae isolates from stool of children with diarrhea symptoms were examined for virulence-associated properties: production of cytotoxic and cytotonic toxins, and invasive ability. most of a. caviae strains were cytotoxic to vero and cho cells and produced cytotonic toxins which caused elongation of cho cells. moreover, five of a. caviae strains revealed invasive ability towards hep-2 cells. | 2003 | 14743980 |
| high-efficiency expression of prolyl endopeptidase from aeromonas punctata subsp. punctata in escherichia coli. | the open reading frame (orf) of prolyl endopeptidase gene from aeromonas punctata subsp. punctata (appep) was amplified by pcr in three parts. the amplified dna segments were ligated to form the complete appep gene, and then cloned into expression vectors pbl (temperature inducible) and pkkh (iptg inducible), respectively. after induction, the expression amounts of recombinant appep in bl21/pkkh-pep and bl21/pbl-pep were about 30% of the total bacterial proteins, and the enzyme activities were 1 ... | 2000 | 12098799 |
| vector potential of houseflies for the bacterium aeromonas caviae. | houseflies, musca domestica linnaeus (diptera: muscidae), have been implicated as vectors or transporters of numerous gastrointestinal pathogens encountered during feeding and ovipositing on faeces. the putative enteropathogen aeromonas caviae (proteobacteria: aeromonadaceae) may be present in faeces of humans and livestock. recently a. caviae was detected in houseflies by pcr and isolated by culture methods. in this study, we assessed the vector potential of houseflies for a. caviae relative to ... | 2002 | 12109714 |
| cloning and nucleotide sequencing of prolyl endopeptidase gene from aeromonas punctata subsp. punctata. | prolyl endopeptidase activity was found in aeromonas punctata subsp. punctata. the genomic dna was partially digested with ecori and the recovered 8-16 kb dna fragments were inserted into the ecori site of plasmid puc19, and were transformated into escherichia coli dh5alpha. the resulted clones were screened by using benzyloxycarbonyl-gly-pro-beta-naphthylamide, the specific substrate of prolyl endopeptidase and a positive clone was obtained. the 12 kb insertion fragment of recombinant plasmid w ... | 1999 | 12114972 |
| the role of the n-terminal propeptide of the pro-aminopeptidase processing protease: refolding, processing, and enzyme inhibition. | pro-aminopeptidase processing protease (pa protease) is an extracellular zinc metalloprotease produced by aeromonas caviae t-64 and it is classified as m04.016 according to the merops database. the precursor of pa protease consists of four regions; a signal peptide, an n-terminal propeptide, a c-terminal propeptide, and the mature pa protease. the in vitro refolding of the intermediate pro-pa protease containing the c-terminal propeptide (mc) was investigated in the presence and absence of the n ... | 2002 | 12147230 |
| in vivo evolution of the aeromonas punctata polyhydroxyalkanoate (pha) synthase: isolation and characterization of modified pha synthases with enhanced activity. | in vivo random mutagenesis of the polyhydroxyalkanoate (pha) synthase gene from aeromonas punctata was performed employing the mutator strain escherichia coli xl1-red. about 200,000 mutants were screened on nile red-containing medium and five mutants with enhanced fluorescence were selected. four of these mutants exhibited enhanced in vivo and in vitro pha synthase activity. mutant m1, which carried the single mutation f518i, showed a five-fold increase in specific pha synthase activity, whereas ... | 2002 | 12172613 |
| effect of superatmospheric oxygen packaging on sensorial quality, spoilage, and listeria monocytogenes and aeromonas caviae growth in fresh processed mixed salads. | atmospheres with o2 levels higher than 70 kpa have recently been suggested as an innovation to modified atmosphere packaging (map) for fresh processed vegetables to maintain sensory quality and safety. in the present work, mixed vegetable salad collected from a commercial processing plant and stored with the map technique was studied. two gas mixtures were actively generated by using an initial o2 concentration of 95 kpa and combined with two plastic films. the low-barrier film permeability for ... | 2002 | 12380740 |
| crystal structure of the (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis. | the (r)-specific enoyl coenzyme a hydratase ((r)-hydratase) from aeromonas caviae catalyzes the addition of a water molecule to trans-2-enoyl coenzyme a (coa), with a chain-length of 4-6 carbons, to produce the corresponding (r)-3-hydroxyacyl-coa. it forms a dimer of identical subunits with a molecular weight of about 14,000 and is involved in polyhydroxyalkanoate (pha) biosynthesis. the crystal structure of the enzyme has been determined at 1.5-a resolution. the structure of the monomer consist ... | 2003 | 12409309 |
| molecular typing of aeromonas isolates in natural mineral waters. | a total of 103 isolates of aeromonas spp. were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic dna with xbai and by pulsed-field gel electrophoresis. all aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among aeromonas hydrophila isolates. these tw ... | 2003 | 12514065 |
| [gene cloning and expression of prolyl endopeptidase from aeromonas punctata]. | prolyl endopeptidase gene was cloned from aeromonas punctata subsp. punctata(st-78-3-3) using activity screening method and the 3.3 kb dna fragment containing pep gene was sequenced. dna sequence from 20-2092 bp was orf region coding 690 amino acids of prolyl endopeptidase. it was a new pep gene through gene search. the genetic engineered e. coli bl21/pgem-pep overexpressing recombinant aeromonas punctata prolyl endopeptidase(appep) was constructed. cultured in yh medium, the expressed appep was ... | 2000 | 12548992 |
| the genetics of glycosylation in gram-negative bacteria. | in recent years there has been a dramatic increase in reports of glycosylation of proteins in various gram-negative systems including neisseria meningitidis, neisseria gonorrhoeae, campylobacter jejuni, pseudomonas aeruginosa, escherichia coli, caulobacter crescentus, aeromonas caviae and helicobacter pylori. although this growing list contains many important pathogens (reviewed by benz and schmidt [mol. microbiol. 45 (2002) 267-276]) and the glycosylations are found on proteins important in pat ... | 2003 | 12586395 |
| general function of n-terminal propeptide on assisting protein folding and inhibiting catalytic activity based on observations with a chimeric thermolysin-like protease. | pro-aminopeptidase processing protease (pa protease) is a thermolysin-like metalloprotease produced by aeromonas caviae t-64. the n-terminal propeptide acts as an intramolecular chaperone to assist the folding of pa protease and shows inhibitory activity toward its cognate mature enzyme. moreover, the n-terminal propeptide strongly inhibits the autoprocessing of the c-terminal propeptide by forming a complex with the folded intermediate pro-pa protease containing the c-terminal propeptide (mc). ... | 2003 | 12589825 |
| cloning, dna sequence, and expression of aeromonas caviae ws7b chitinase gene. | a chitinase-producing bacterium, designated ws7b, was isolated from a soil sample obtained from a black-pepper plantation on bangka island, indonesia. fatty-acid methyl-ester analysis indicated that the isolate was aeromonas caviae. a chitinase gene from ws7b was cloned in a puc19-based plasmid vector, but without its natural promoter. the complete nucleotide sequence of the gene was determined, and the structural gene consisted of a 2748-bp region encoding 864 amino acids. dna sequence analysis ... | 2003 | 12611264 |
| bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates. | 30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ... | 2003 | 12644237 |