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very early presentation of pompe's disease and its cross-sectional echocardiographic features.pompe's disease was diagnosed in a 23-day-old female infant with congestive cardiac failure and hypotonia. the cross-sectional echocardiographic features of this case are described. the possible implications of this extremely early presentation are discussed.20123308716
[protein studies in cystic and peritoneal fluids of ovarian tumors with special reference to cea].the levels of total protein, ig, afp, beta-hcg, cea, sc, sp1 and alpha 2-pag were measured in cystic and peritoneal effusions obtained from 70 females with benign and malignant ovarian tumors. this study was carried out to test out parameters those are useful for tumor monitoring of ovarian cancer. our results indicate that determining cea in peritoneal effusions could be one of useful methods in tumor monitoring of mucinous cystadenocarcinomas in cases of known histology and praetherapeutic est ...20123376613
culturable gut microbiota diversity in zebrafish.the zebrafish (danio rerio) is an increasingly used laboratory animal model in basic biology and biomedicine, novel drug development, and toxicology. the wide use has increased the demand for optimized husbandry protocols to ensure animal health care and welfare. the knowledge about the correlation between culturable zebrafish intestinal microbiota and health in relation to environmental factors and management procedures is very limited. a semi-quantitative level of growth of individual types of ...201222428747
[february 1948 and the further development of czechoslovak pharmacy]. 20123282688
have you looked at your will lately? seven common mistakes in estate planning and how to avoid them. 20123373137
[properties of lak cell activity and lak-generation in pbl isolated from patients with renal cell carcinoma]. 20123266646
wine, women, suspiciousness and advertising.women college students (n = 66) viewed videotapes of a soap opera episode and a rock music program, ostensibly to evaluate the appeal of such material to college women. different versions of the videotape included zero, three or nine wine commercials. refreshments, including white wine, were available to the subjects. suspiciousness about the experiment was evaluated through an open-ended questionnaire, responses to which were independently content-analyzed by two scorers. major findings were as ...20123560952
wine, women, suspiciousness and advertising.women college students (n = 66) viewed videotapes of a soap opera episode and a rock music program, ostensibly to evaluate the appeal of such material to college women. different versions of the videotape included zero, three or nine wine commercials. refreshments, including white wine, were available to the subjects. suspiciousness about the experiment was evaluated through an open-ended questionnaire, responses to which were independently content-analyzed by two scorers. major findings were as ...20123560952
[how many people undergo treatment with a psychiatrist?]. 20123648811
[how many people undergo treatment with a psychiatrist?]. 20123648811
astigmatism after strabismus surgery. 20123347457
diathermy burns. 20123696207
use of computerized data listings and activity profiles of genetic and related effects in the review of 195 compounds.computer-generated listings of data from short-term tests for genetic and related effects (activity profile listings) were prepared for 195 compounds that included for each compound, the test system (identified by a three-letter code word), qualitative results and the lowest effective dose (led) or highest ineffective dose (hid) tested. a corresponding bar or line graph (activity profile) was also generated, in which test systems are displayed along the x-axis and the led or hid values along the ...20123367921
a word about words. 20123459834
malignant hyperthermia in a renal transplant recipient. 20123318564
infection caused by aeromonas sobria, complicated by lower extremity paraplegia and the cauda equine syndrome in a patient with well-controlled type 2 diabetes. 201223671455
[pit and fissure sealants. the effect of etching depends on the differences in tooth surface cleaning methods, and the method of cleaning pits and fissures]. 20123526496
radiographic assessment of knee alignment after total knee arthroplasty.standard anteroposterior standing radiographs (14 x 17-inch cassettes) and full-limb radiographs (51-inch) from a randomized group of 50 patients undergoing total knee arthroplasty between january 1985 and june 1986 were analyzed. the mean tibiofemoral angle on short films was 5.8 degrees valgus, compared with 7.2 degrees valgus on full-limb films. this 1.4 degrees discrepancy had a standard deviation of 2.2 degrees, emphasizing the measurement variability in any individual case. the distance fr ...20113361322
aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet.two freshwater isolates (wb4.1-19(t) and wb4.4-101), sharing 99.9 % 16s rrna gene sequence similarity, were highly related to aeromonas sobria (99.7 % similarity; 6 bp differences). a phylogenetic tree derived from a multi-locus phylogenetic analysis (mlpa) of the concatenated sequences of five housekeeping genes (gyrb, rpod, reca, dnaj and gyra; 3684 bp) revealed that both strains clustered as an independent phylogenetic line next to members of aeromonas molluscorum and aeromonas bivalvium. the ...201120207806
bcr-abl oncogene renders myeloid cell line factor independent: potential autocrine mechanism in chronic myeloid leukemia.in chronic myeloid leukemia (cml), a chromosome translocation has fused the bcr gene to the c-abl oncogene, such that a chimeric bcr-abl polypeptide can be made. to explore the biological properties of bcr-abl and compare them with those of the abelson virus (amulv) transforming gene (gag-v-abl), we have used either a synthetic bcr-v-abl gene that mimics the translocation product or, in some experiments, a bcr-c-abl cdna. a new retroviral vector was used to introduce the genes into the factor-de ...20113147434
quorum sensing in aeromonas species isolated from patients in malaysia.bacterial quorum sensing signal molecules called n-acylhomoserine lactone (ahl) controls the expression of virulence determinants in many gram-negative bacteria. we determined ahl production in 22 aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). all isolates produced the two principal ahls, n-butanoylhomoserine lactone (c4-hsl) and n-hexanoyl homoserine lactone (c6-hsl). ten isolates also produced additional ahls. this re ...201120544198
identification and pathogenicity of aeromonas sobria on tail-rot disease in juvenile tilapia oreochromis niloticus.thirty-six strains, numbered from py01 to py36, were isolated from six moribund oreochromis niloticus. the biochemical characteristics of all strains conformed to the species description of aeromonas sobria on the basis of api 20e and biolog gn system. furthermore, gyrb sequence of strain py36 was sequenced and showed high similarity (99.8%) with a. sobria in genbank. antibiotic-resistance of strain py36 was assessed by the kirby-bauer disk diffusion method, and the results showed it was suscept ...201120853167
inhibition of biosynthesis of metalloprotease of aeromonas sobria by sodium chloride in the medium.the present authors have previously shown that the serine protease activity of aeromonas sobria is markedly decreased when a. sobria is cultured in medium containing 3.0% sodium chloride (nacl, concentration almost equivalent to sea water salinity), and that this occurs because, although the synthesis of asp is not disturbed by the salt in the medium, the maturation pathway of serine protease of a. sobria (asp) does not proceed successfully in such a medium. in this study, the effect of salt in ...201121175775
the influence of environmental bacteria in freshwater stingray wound-healing.invasion by bacteria can influence the course of healing of wounds acquired in aquatic environment. in this study, the bacteria present in potamotrygonmotoro stingray mucus and in the alto paraná river water were identified, and their ability to induce tissue injury and resist antibiotics was determined. biochemical identification analysis showed that 97% of all bacterial isolates were gram negative, aeromonas spp., enterobacter cloacae and citrobacter freundii being the species most prevalent. ...201121635911
Structural and Functional Analysis of the Serine Protease from Aeromonas sobria.Aeromonas species are Gram-negative facultative anaerobic bacteria found ubiquitously in a variety of aquatic environments and most commonly implicated as causative agents of gastroenteritis. Sepsis is a fatal complication of Aeromonas infectious diseases, particularly in immune-compromised patients. Two species, Aeromonas hydrophila and Aeromonas sobria, are associated with human disease. Feasible virulence factors of Aeromonas include fimbrial and afimbrial adhesion molecules, hemolysins, ente ...201122129876
[fatal aeromonas sobria infection in liver cirrhosis].aeromonas sobria infection is known to be very serious in immunocompromised hosts. we report a case of a. sobria infection fatal in a subject with decompensated liver cirrhosis. a 63-year-old man with liver cirrhosis admitted for fever and vomiting developed a necrotizing soft-tissue infection in the right lower leg. despite a decompression incision in the affected limb and intensive care, he died of sepsis one day after surgery. a. sobria was detected afterward in a blood culture.201122117380
[new aspects of vitamin k prophylaxis in newborn infants and infants].discussion about vitamin k prophylaxis has again become a matter of major concern since cases of life threatening bleeding due to late onset vitamin k deficiency have been observed in germany. this review tries to summarize present knowledge about the clinical presentation, pathogenesis and the potential prevention of vitamin k deficiency caused bleeding in the neonatal period and infancy.20113785234
bioaffinity sensors. 20113131631
pattern-evoked cortical potentials and nuclear magnetic resonance imaging in total and partial agenesis of the corpus callosum.we have examined two cases of agenesis of the corpus callosum without any associated abnormalities. they were accidentally detected in x-ray-computed tomography. total agenesis of the corpus callosum could be differentiated by nuclear magnetic resonance imaging from partial agenesis. they had moderately depressed stereopsis. visually evoked cortical potentials to pattern stimuli showed abnormal findings in binocular viewing, and vecp equi-potential maps were similar to those of normal controls.20114053962
[lumbalgia among adolescents]. 20113158780
darkroom diseases and how to combat them. 20113112384
aeromonas sobria bacteriemia in an acute lymphoblastic leukemia case in remission. 201127263949
[preparation and identification of ompw monoclonal antibodies].to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw.201121906472
three goose-type lysozymes in the gastropod oncomelania hupensis: cdna sequences and lytic activity of recombinant proteins.three goose-type (g-type) lysozymes, designated as ohlysg1, ohlysg2 and ohlysg3 were identified from expressed sequence tags (ests) of a gastropod oncomelania hupensis, the intermediate host of schistosoma japonicum. the full cdna sequences of ohlysg1, ohlysg2 and ohlysg3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the n-terminus, respectively. the three g-type lysozymes shared conserv ...201121756934
post-translational processing of anglerfish islet somatostatin precursors.two distinct somatostatin precursors are synthesized in anglerfish (af) islets. in addition to a precursor which has somatostatin 14 (ss-14) as a c-terminal cleavage product, a precursor which contains at its c-terminus [tyr7, gly10] ss-14 as a potential cleavage product is also synthesized. however, even though an arg-lys pair is located immediately n-terminal to ala1 of the c-terminal tetradecapeptide, [tyr7, gly10]ss-14 was not found in significant amounts in extracts of af islets. instead, a ...20102863927
raising the standards. the right tools for the job. 20103405819
studies on 2(1h)-quinolinone derivatives as neuroleptic agents i. synthesis and biological activities of (4-phenyl-1-piperazinyl)-propoxy-2(1h)-quinolinone derivatives. 20102907733
simple and direct detection of aeromonas hydrophila infection in the goldfish, carassius auratus (l.), by dot blotting using specific monoclonal antibodies.a combination of eight isolates of aeromonas hydrophila was used to produce monoclonal antibodies (mabs). ten different groups of mabs specific to aeromonas were selected. the first five groups of mabs demonstrated high specificity and bound to only one or two isolates of a. hydrophila. the sixth and the seventh groups of mabs were a. hydrophila specific. they recognized seven of eight a. hydrophila isolates (ah1, 2, 3, 4, 5, 6, 8); however, the mab in the seventh group also showed cross-reactiv ...201021091724
maturation pathway of metalloprotease produced by aeromonas sobria.previously, we cloned the metalloprotease gene of aeromonas sobria (amp) and determined its nucleotide sequence (genbank accession number dq784565). the protease is composed of 591 amino acid residues. in this study, we purified the mature metalloprotease from the culture supernatant of a. sobria and determined the amino terminal sequence and molecular size of amp. in addition, we examined the production of amp diachronically and found that amp emerges outside of the cell as an intermediate comp ...201021118297
enhancement of protective immunity in european eel (anguilla anguilla) against aeromonas hydrophila and aeromonas sobria by a recombinant aeromonas outer membrane protein.to develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (omp) antigen from different bacterial pathogens. in this study, an omp fragment of 747 bp (named as omp-g), which was highly conserved in seven aeromonas omp sequences from the ncbi database, was amplified by pcr from one aeromonas sobria strain (b10) and two aeromonas hydrophila strains (b27 and b33) with the designed specific prim ...201021148192
aeromonas fluvialis sp. nov., isolated from a spanish river.a gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(t), was isolated from a water sample collected from the muga river, girona, north-east spain. preliminary analysis of the 16s rrna gene sequence showed that this strain belonged to the genus aeromonas, the nearest species being aeromonas veronii (99.5 % similarity, with seven different nucleotides). a polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrb, rpod, reca, dnaj and g ...201019648345
cellular components of probiotics control yersinia ruckeri infection in rainbow trout, oncorhynchus mykiss (walbaum).subcellular components of the probiotics aeromonas sobria gc2 and bacillus subtilis jb-1, when administered to rainbow trout, oncorhynchus mykiss, conferred protection against a new biogroup of yersinia ruckeri. thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (cwps), outer membrane proteins (omps), lipopolysaccharides (lps), whole cell proteins (wcps) and live cells followed by challenge on day 8 with y. ruckeri led to 80-100% survival compared with 10% ...201019912460
association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of anodonta cygnea (linnaeus, 1758).bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. a complete understanding of the balance between anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of a. cygnea collected from mira lagoon in northern portugal. the results showed vibrio metschnikovii and aeromonas sobria as the dominant groups. th ...201020349058
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
malignant melanoma. 20103768643
monoclonal antibodies as carriers of toxins. 20092785694
more on index of refraction. 20092805311
more on index of refraction. 20092805311
clinical presentations, prognostic factors, and mortality in patients with aeromonas sobria complex bacteremia in a teaching hospital: a 5-year experience.bacteremia due to aeromonas sobria complex is an uncommon clinical presentation, associated with a high mortality rate. this retrospective study reviewed the clinical manifestations and prognostic factors of a. sobria complex bacteremia.200920422137
repeated hepatic ischemia as a treatment for carcinoid liver metastases.hepatic ischemia has been used in the treatment of bilobar malignant carcinoid tumors in the liver. hepatic artery ligation, hepatic dearterialization, and embolization with nondegradable micromaterial have been followed by collateral formation and are associated with a high complication rate. to reduce this, an implantable vascular occluder permitting intermittent occlusion of the hepatic artery was used in 3 patients with bilateral malignant carcinoid in the liver, with high urinary excretion ...20092741468
lead toxicity and iron deficiency in utah migrant children.we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening.20092650572
the carboxy-terminal tail of aeromonas sobria serine protease is associated with the chaperone.asp is the only bacterial protease in the kexin group of the subtilisin family. previous studies have revealed that the orf2 protein encoded at the 3' end of the asp operon is required for asp to change from a nascent form into an active form in the periplasm. however, the mechanism by which orf2 makes contact and interacts with asp in the maturation process remains unknown. the present study examined the effect of mutations in the carboxy-terminal region of asp on the asp maturation process. bo ...200919954452
rosmarinus officinalis l.: chemical modifications of the essential oil and evaluation of antioxidant and antimicrobial activity.rosmarinus officinalis essential oil was separated into its hydrocarbon and oxygenated fractions. the major compounds in the hydrocarbon fraction were alpha-pinene (44.2%), camphene (24.5%), and limonene (11.7%), while in the oxygenated fraction they were 1,8-cineole (37.6%), camphor (16.5%), and bornyl acetate (21.4%). the hydrocarbon fraction was submitted to a hydroformylation process and the antioxidant activity of the product was screened by the dpph and beta-carotene/linoleic acid tests. t ...200920120107
structural basis for the kexin-like serine protease from aeromonas sobria as sepsis-causing factor.the anaerobic bacterium aeromonas sobria is known to cause potentially lethal septic shock. we recently proposed that a. sobria serine protease (asp) is a sepsis-related factor that induces vascular leakage, reductions in blood pressure via kinin release, and clotting via activation of prothrombin. asp preferentially cleaves peptide bonds that follow dibasic amino acid residues, as do kex2 (saccharomyces cerevisiae serine protease) and furin, which are representative kexin family proteases. here ...200919654332
a novel method for the diagnosis of drowning by detection of aeromonas sobria with pcr method.the acid digestion method has been widely used for the diagnosis of death by drowning, but it is not always sensitive. however, there has been no definitive method to replace acid digestion until now. we speculate that bacteria are more useful markers than plankton for the diagnosis of death by drowning. in this study, from the preserved blood samples of 32 freshwater drowning cases, specific dna fragments of aeromonas sobria, one of the most common aquatic bacteria, were examined using pcr. the ...200919766051
case reports: fatal necrotizing fasciitis caused by aeromonas sobria in two diabetic patients.we report two rare cases of aeromonas sobria necrotizing fasciitis with sepsis in patients with diabetes. in both cases, immediate fasciotomy was performed and appropriate empiric antimicrobial therapy and intensive care were administered. however, the two patients died on day 2 and day 11, respectively, after admission as a result of multiple organ failure. when patients present with a rapid onset of skin necrosis and progressive sepsis, an aeromonas sobria infection or vibrio infection should ...200918800212
aeromonas sobria sepsis complicated by rhabdomyolysis in an hiv-positive patient: case report and evaluation of traits associated with bacterial virulence.human infection with aeromonas species is uncommon and most often due to trauma with exposure to contaminated water or soil. a 43-year-old hiv- and hepatitis c virus (hcv)-infected male, after a two-week course of corticosteroid therapy for an autoimmune anemia, developed diarrhea, dermatologic manifestations and a multiple organ dysfunction syndrome, resulting in death. although stool samples were repeatedly negative, two sets of blood cultures obtained during a single peak of fever yielded the ...200918977680
appendicitis associated with travelers' diarrhea caused by aeromonas sobria.aeromonas species is an infrequent pathogen causing travelers' diarrhea and gastroenteritis. no human case of appendicitis has been reported as a complication of aeromonas infection until now. we describe a case of acute appendicitis associated with aeromonas sobria infection acquired in cebu, philippines.200919335814
pseudomonas chlororaphis strain jf3835 reduces mortality of juvenile perch, perca fluviatilis l., caused by aeromonas sobria.motile aeromonad septicaemia caused by aeromonas sobria is a cause of disease in farmed perch, perca fluviatilis l., in switzerland. we have evaluated the potential of a pseudomonas chlororaphis isolate, obtained from perch intestine, to control a. sobria infection. inoculation of juvenile perch with p. chlororaphis strain jf3835 prior to infection with a. sobria caused a reduction in a. sobria associated mortalities. infection of perch with xyle-labelled p. chlororaphis indicated the bacterium ...200919476558
toxin production by aeromonas sobria in natural environments: river water vs. seawater.aeromonas are water-borne pathogens. they are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% nacl). however, the presence of aeromonas in seawater is extremely rare compared with that in river water. in this study, we tested the ability of aeromonas sobria to produce toxins in river water and seawater. first, we cultured a. sobria on skim milk agar plates supplemented with either river water (sarw) or seawater (sasw). the ...200819122681
cystic neoplasms of the pancreas.eight patients with cystic neoplasms of the pancreas were seen at four northern california hospitals between the years 1978 and 1986. three of the tumors were benign and five were malignant. three females, whose average age was 61 years, had cystadenomas. three females and two males, whose average age was 48 years, had mucinous cystadenocarcinomas. clinical presentations were similar among all patients. abdominal pain was a prominent feature. anorexia, weight loss, nausea and vomiting with a pal ...20082446398
a randomized trial of anticoagulation with warfarin and of alternating chemotherapy in extensive small-cell lung cancer by the cancer and leukemia group b.the cancer and leukemia group b (calgb) conducted a prospective randomized trial to evaluate the role of warfarin and alternating chemotherapy in extensive small-cell lung cancer (sccl). after stratification for sex and performance status, patients were randomly assigned to receive chemotherapy with methotrexate, doxorubicin (adriamycin; adria laboratories, columbus, oh), cyclophosphamide, and lomustine (ccnu) (macc), or macc plus warfarin (macc + w), or mitomycin, etoposide, cisplatin, and hexa ...20082547030
enrolled nurses. we're backing the charter! 20082587373
non-germ cell testicular tumours in nigerians.non-germ cell testicular tumours are reported to be more common in peoples of negroid and asian descent than in caucasians. this group of tumours comprised seven of sixteen testicular tumours seen in a ten-year period at the university of benin teaching hospital, benin city, nigeria. however, unlike experiences elsewhere in africa and the west indies where paratesticular tumours accounted for a majority of these tumours, malignant lymphomas constituted over 70% of non-germinal testicular tumours ...20082635452
proteomic analysis of rainbow trout (oncorhynchus mykiss, walbaum) serum after administration of probiotics in diets.the response of rainbow trout (oncorhynchus mykiss, walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (apr). proteomic analysis by two-dimensional electrophoresis (2d) concurrently with mass spectrometry was used to detect apr related proteins in rainbow trout serum following feeding with probiotics aeromonas sobria gc2 and bacillus sp. jb-1. three candidate proteins increased following use of gc2, and wer ...200817981340
non-germ cell testicular tumours in nigerians.non-germ cell testicular tumours are reported to be more common in peoples of negroid and asian descent than in caucasians. this group of tumours comprised seven of sixteen testicular tumours seen in a ten-year period at the university of benin teaching hospital, benin city, nigeria. however, unlike experiences elsewhere in africa and the west indies where paratesticular tumours accounted for a majority of these tumours, malignant lymphomas constituted over 70% of non-germinal testicular tumours ...20082635452
fluid secretion caused by aerolysin-like hemolysin of aeromonas sobria in the intestines is due to stimulation of production of prostaglandin e2 via cyclooxygenase 2 by intestinal cells.to clarify the mechanisms of diarrheal disease induced by aeromonas sobria, we examined whether prostaglandin e2 (pge2) was involved in the intestinal secretory action of a. sobria hemolysin by use of a mouse intestinal loop model. the amount of pge2 in jejunal fluid and the fluid accumulation ratio were directly related to the dose of hemolysin. the increase over time in the level of pge2 was similar to that of the accumulated fluid. in addition, hemolysin-induced fluid secretion and pge2 synth ...200818086811
identification of bacteria from the normal flora of perch, perca fluviatilis l., and evaluation of their inhibitory potential towards aeromonas species.pathogenic aeromonas sobria has been identified as a causative agent of ulcerative disease in farmed european perch, perca fluviatilis l. to study the effect of the normal intestinal bacterial flora of perch against a. sobria, we sampled 193 bacterial isolates from the perch digestive tract. the isolates were identified by sequence analysis of the 16s rrna gene and their inhibitory potential against a. sobria was evaluated in vitro. nineteen of the strains isolated showed inhibition and were als ...200818355180
efficacy of in-feed probiotics against aeromonas bestiarum and ichthyophthirius multifiliis skin infections in rainbow trout (oncorhynchus mykiss, walbaum).the aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by aeromonas bestiarum and ichthyophthirius multifiliis (ich) in rainbow trout.200818422955
impaired plasma clottability induction through fibrinogen degradation by asp, a serine protease released from aeromonas sobria.aeromonas sobria infection often advances to sepsis, in which interaction of bacterial components with plasma proteins possibly causes various disorders. this bacterium releases a serine protease (asp), a putative virulence factor, and binds to fibrinogen. to study the asp effect on fibrinogen, we incubated fibrinogen or plasma with asp and investigated their clotting elicited by thrombin, which converts fibrinogen to a fibrin clot. enzymatically active asp retarded plasma clotting in a dose-dep ...200818462393
[characterization of aeromonas spp isolates from newborns hospitalized].aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ...200818545840
production of c5a by asp, a serine protease released from aeromonas sobria.aeromonas sobria causes pus and edema at sites of infection. however, the mechanisms underlying these effects have not been elucidated. c5a, the amino-terminal fragment of the complement 5th component (c5), mimics these events. to investigate the involvement of c5a in the pathophysiology of a. sobria infection, we examined release of c5a from human c5 by a serine protease (asp), a putative virulence factor secreted by this bacterium. c5 incubated with enzymatically active asp induced neutrophil ...200818714034
clinical significance and distribution of putative virulence markers of 116 consecutive clinical aeromonas isolates in southern taiwan.the objectives of this study were to elucidate the clinical manifestations of aeromonas infections and the association of putative virulence genes with clinical invasiveness.200716716402
aeromonas hydrophila and aeromonas sobria bacteremia: rare pathogens of infection in a burn patient. 200717084982
induction of apoptosis in vero cells by aeromonas veronii biovar sobria vacuolating cytotoxic factor.recently, a cytotoxin named vacuolating cytotoxic factor (vcf) in aeromonas sobria and aeromonas veronii biovar sobria was described. we have now purified this factor using ion metallic affinity chromatography. the vcf is a nonhemolytic enterotoxin that acts as a serine protease. the toxin can be partially neutralized by serum antiaerolysin and it induced not only cytoplasmatic vacuole formation, but also mitochondrial disorders that must be signaling the apoptotic pathways, leading to vero (afr ...200717286562
metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake.in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ...200717406774
the immune response and protective efficacy of oral alginate microparticle aeromonas sobria vaccine in soft-shelled turtles (trionyx sinensis).aeromonas sobria is an important bacterial pathogen of soft-shelled turtles (trionyx sinensis). the effect of an oral alginate microparticle a. sobria vaccine (mav) on immune response and protection efficacy of soft-shelled turtles was investigated. the formalin-killed cultures of a. sobria strain z-1 (fkc) were incorporated into alginate microparticles, and the encapsulated vaccine was orally used for immunizing turtles. the serum agglutinating antibody titers, bactericidal activity of blood le ...200717628697
the immune response and protective efficacy of vaccination with oral microparticle aeromonas sobria vaccine in mice.the effect of an oral alginate microparticle aeromonas sobria vaccine on immune response and protection of mice was investigated. the formalin-killed cultures (fkc) of a. sobria strain z-1 treated with three methods were incorporated into alginate microparticles, and the encapsulated vaccines were orally used for immunizing mice. four weeks after immunization, the serum agglutinating antibody titers, bactericidal activity of leucocytes and relative percent survival (rps) of mice immunized orally ...200717630205
rapid detection of virulence factors of aeromonas isolated from a trout farm by hexaplex-pcr.the detection of virulence factors of aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. in this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and aeromonas species were isolated and identified. for rapid detection of six virulence factors of isolated aeromonas, a hexaplex-polymerase chain reaction (hexaplex-pcr) assay was used. the detected virulence factors include a ...200717846582
the development of probiotics for the control of multiple bacterial diseases of rainbow trout, oncorhynchus mykiss (walbaum).jb-1 and gc2, which were equated with bacillus sp. and aeromonas sobria respectively, were recovered from the digestive tract of rainbow trout, oncorhynchus mykiss and ghost carp, cyprinus sp. respectively, and demonstrated effectiveness as probiotics for the control of infections caused by aeromonas salmonicida, lactococcus garvieae, streptococcus iniae, vibrio anguillarum, vibrio ordalii and yersinia ruckeri. when administered to rainbow trout (average weight = 12 g) for 14 days in feed dosed ...200717850573
salt in surroundings influences the production of serine protease into milieu by aeromonas sobria.previously we have shown that the open reading frame 2 protein (orf2 protein), which is encoded at the 3 ' end of serine protease of aeromonas sobria (asp), functions as a chaperone protein in periplasm in the production of asp. both proteins, asp and orf2 protein, associate in periplasm and orf2 protein helps asp to take an active form. asp which is dissociated from orf2 protein emerges in milieu . in this study, we examined the effect of sodium chloride (nacl) in medium on asp production by a. ...200717951986
aeromonas sobria prostatitis and septic shock in a healthy man with chronic alcoholic consumption.prostate infection caused by aeromonas sobria has not been reported in the literature. we presented a 44-year-old healthy man with a habit of alcoholic consumption who was admitted because of primary a. sobria prostatitis with septicemia. the patient was successfully treated with intravenous ofloxacin and was uneventful after a half-year follow-up. based on our rare case, we suggest to clinical physicians that prostatitis might be considered in healthy male patients with a. sobria bacteremia and ...200718032845
activation of prothrombin by asp, a serine protease released from aeromonas sobria.the effect of a serine protease (asp) secreted from aeromonas sobria on plasma coagulation was investigated. proteolytically active asp promoted human plasma coagulation in a dose-dependent manner. consistent with the preference for a factor xa-specific oligo-peptide substrate, asp produced enzymatic activity from human prothrombin but not from factors ix and x. asp cleaved prothrombin to produce enzymatically active 37 kda-fragment displaying the same molecular mass as alpha-thrombin. asp is th ...200718067862
molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein.lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ...200717850883
mediator and cellular mechanisms in asthma. the philip ellman lecture 1990. 20072258848
memory and anergy: challenges to traditional models of t lymphocyte differentiation.traditional paradigms suggest that encounter with an antigen converts naive peripheral t cells into memory cells with less stringent requirements for activation and increased capacities for lymphokine production. recent evidence argues that this view may be over-simplified in two ways. first, an encounter with antigen in the absence of certain costimulatory factors can render a t cell anergic--that is, unable to respond to antigen under normal conditions. second, although cells of the memory t c ...20061563595
a high containment polymodal pilot-plant fermenter--design concepts.a 225 dm3 pilot-plant bioreactor system has been designed and constructed that is suitable for biohazardous fermentations. the design enables operation at containment levels above the requirements of good industrial large-scale practice (gilsp) without secondary containment of the whole plant. the main biosafety features of the systems include the use of steam barriers on o-ring seals, supply lines and stirrer seals, multiple o-ring seals, piping of condensate lines and pressure relief systems t ...20061367227
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
[a just pension system]. 20061636316
aeromonas sobria hemolysin causes diarrhea by increasing secretion of hco3-.aeromonas sobria hemolysin (ash) is one of the major virulence factors produced by a. sobria, a causative agent of diarrhea in humans. we investigated the effects of ash on anion transport in human colonic epithelial cells. ash increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external hco3-. iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. ...200616630261
induction of vascular leakage and blood pressure lowering through kinin release by a serine proteinase from aeromonas sobria.aeromonas sobria causes septic shock, a condition associated with high mortality. to study the mechanism of septic shock by a. sobria infection, we examined the vascular leakage (vl) activity of a. sobria serine proteinase (asp), a serine proteinase secreted by this pathogen. proteolytically active asp induced vl mainly in a bradykinin (bk) b(2) receptor-, and partially in a histamine-h(1) receptor-dependent manner in guinea pig skin. the asp vl activity peaked at 10 min to 1.8-fold of the initi ...200617142774
aeromonas sharmana sp. nov., isolated from a warm spring.a gram-negative, facultatively anaerobic bacterial strain designated gptsa-6(t) was isolated from a water sample collected from a warm spring in assam, india. preliminary analysis of the 16s rrna gene sequence of this isolate revealed its affiliation to the family aeromonadaceae. detailed characterization using a polyphasic approach indicated that strain gptsa-6(t) is most closely related to aeromonas sobria but differs significantly from existing members of the genus aeromonas. analysis of the ...200616902029
mechanistic studies on the mononuclear znii-containing metallo-beta-lactamase imis from aeromonas sobria.in an effort to understand the reaction mechanism of a b2 metallo-beta-lactamase, steady-state and pre-steady-state kinetic and rapid freeze quench electron paramagnetic resonance (epr) studies were conducted on imis and its reaction with imipenem and meropenem. ph dependence studies revealed no inflection points in the ph range of 5.0-8.5, while proton inventories demonstrated at least 1 rate-limiting proton transfer. site-directed mutagenesis studies revealed that lys224 plays a catalytic role ...200616939225
cleavage specificity of the serine protease of aeromonas sobria, a member of the kexin family of subtilases.subtilisin-like proteases have been grouped into six families based on a sequence of the catalytic domain. one of the six is the kexin family, of which furin is a representative protease. all members of the kexin family, except one, are from eukaryotes. the one prokaryotic protease is a serine protease of aeromonas sorbria (asp). here, we examined the substrate specificity of asp based on the cleavage of short peptides. the results showed that asp preferentially cleaves the peptide bond followin ...200616487335
[detection of multiresistance aeromonas with tem type resistant genes in patients with cirrhosis].to study the status of beta-lactamase produced by multiresistant aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading.200516201471
recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ...200516269764
use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum).from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ...200516336470
a pore-forming toxin produced by aeromonas sobria activates camp-dependent cl- secretory pathways to cause diarrhea.aeromonas sobria hemolysin (ash) is one of the major virulence factors produced by a. sobria, a human pathogen that causes diarrhea. we investigated the effects of ash on cl(-) transport in human colonic epithelial cells. ash increased short-circuit currents (isc) and (125)i efflux from caco-2 cells, indicating ash activate cl(-) secretion. additions of inhibitors of cyclic amp dependent cl(-) channels, glybenclamide and nppb suppressed the isc and (125)i efflux increases induced by ash. and ash ...200515621437
placental and intestinal alkaline phosphatases are receptors for aeromonas sobria hemolysin.the aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. we previously identified the putative receptor for a. sobria hemolysin as a p66 protein on intestine 407 cells (microb. pathog. 27 (1999) 215-221). here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (plap). recombinant plap expressed in 293t cells was also found to bind to hemolysin and the binding was ...200515715171
antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish.motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ...200515747443
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