Publications

[spectroscopy of the liver in vivo using nuclear magnetic resonance. a new approach in hepatic physiopathology].	nuclear magnetic resonance (nmr) spectroscopy is a non-invasive means of detecting numerous compounds, thus enabling real-time studies to be carried out on such topics as energy metabolism, intracellular ph and main intermediate metabolism pathways. this method has been used for several years to study perfused livers and rat livers in vivo, but its application to man has just begun. we describe some of its principal uses, with emphasis on the possibilities it offers to investigate the metabolic ...	1990	2140172
induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis.		1990	2164982
production and characterization of monoclonal antibodies to aeromonas sobria surface antigens.	a panel of eight murine monoclonal antibodies (mabs) was raised against the surface antigens of aeromonas sobria isolated from a patient with diarrhoea. antibodies to the lps core and o-antigen and to protein antigens were generated. three mabs of the igm isotype against either protein or lps agglutinated 34/75 aeromonas isolates from clinical and environmental sources. similar numbers of a. hydrophila, a. sobria and a. caviae isolates were agglutinated. the mabs were screened for their ability ...	1989	2483346
[intestinal infections caused by aeromonas].	we have identified 38 instances of intestinal infection by aeromonas and 4 by plesiomonas in 16,184 coprocultures carried out during a 4 year period in the hcu of zaragoza. the low prevalence can be related with the fact that specific media for the recovery of these organisms were not used. the species aeromonas sobria (47.36%) and a. caviae (39.47%) were more common than a. hydrophila (13.15%). aeromonas were isolated from patients with and without intestinal disease. the occurrence of gastroin ...	1989	2490673
effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets.	the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ...	1989	2556355
[antibacterial and bactericidal activities of japanese green tea].	we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ...	1989	2677434
fever response in laboratory-maintained and free-ranging chuckwallas (sauromalus obesus).	the fever response was studied in laboratory-maintained and free-ranging chuckwallas. five animals were used in a self-pairing laboratory experiment in which each animal was first injected with a sterile, pyrogen-free saline solution. approximately 7 days later, each individual was injected with a solution of 4.75 x 10(9) aeromonas sobria (formerly a. hydrophila). saline-injected individuals had a mean preferred body temperature (mpbt) of 36.8 +/- 0.4 (se) degrees c, whereas bacteria-injected an ...	1989	2750958
species identification of aeromonas strains based on carbon substrate oxidation profiles.	twenty clinical strains each of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria were evaluated for their abilities to oxidize one or more of 95 carbon sources on a gn microplate (biolog, hayward, calif.). nine substrates yielded good, discriminatory values for the three species tested. the panel appears to be useful for the species identification of aeromonas isolates originating from human material.	1989	2778077
[experimental study of the role of male ixodid ticks in the circulation of the tick-borne encephalitis virus].	regular transmission of the tick-borne encephalitis virus by ixodidae males feeding on laboratory animals is demonstrated. the virus titres in the male saliva were 0.1-1.5 lgld50/0.03 ml. it was established that such doses didn't produce tense viremia in white mice or clinical tick-borne encephalitis.	1989	2779496
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro].		1989	2806660
concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated.	because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ...	1989	2561041
characterization of aeromonas sobria hemolysin by use of monoclonal antibodies against aeromonas hydrophila hemolysins.	aeromonas sobria produces hemolysin in a form activable with trypsin under defined cultural conditions. in immunoblotting analyses with the culture supernatant of a. sobria, the monoclonal antibody reacting specifically to aeromonas hydrophila ca-11 hemolysin bound to the 53,000- and 49,000-dalton bands before and after trypsinization, respectively. the monoclonal antibody reacting to a. hydrophila ah-1 hemolysin did not bind either band. a. sobria hemolysin is, therefore, related antigenically ...	1989	2768466
histamine concentration and ca2+ mobilization in arterial smooth muscle.	smooth muscle contraction is dependent on ca2+ entry from the extracellular space or release from intracellular stores. the sensitivity of these ca2+ sources to agonist concentration was evaluated by measuring myoplasmic [ca2+] (as estimated by aequorin), myosin phosphorylation, and isometric stress in the swine carotid media. high histamine concentrations produced transient elevations in [ca2+] and phosphorylation with rapid generation of near maximal stress. lower histamine concentrations prod ...	1989	2750885
antimicrobial susceptibility of sixty human fecal isolates of aeromonas species.	the mics of 21 antimicrobial agents were determined for 60 strains of aeromonas spp. isolated from human feces. all isolates tested were susceptible to aztreonam, tetracycline, imipenem, moxalactam, pipemidic acid, gentamicin, trimethoprim-sulfamethoxazole, pefloxacin and ciprofloxacin. resistance to erythromycin and streptomycin was observed in all 60 strains. aeromonas caviae was less susceptible to cefamandole, cefotaxime, norfloxacin, chloramphenicol, tetracycline, sulfamethoxazole and trime ...	1989	2496995
hydrogen peroxide mutagenicity towards salmonella typhimurium.	in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ...	1989	2572065
comparative sequence analysis, in vitro expression and biosynthesis of mouse prp.	a number of studies have indicated that an endogenous brain protein, prp, is associated with transmissible agents causing spongiform encephalopathies such as scrapie, kuru, and creutzfeldt-jakob disease. it has been proposed that prp derived from scrapie brain is the scrapie agent itself. to test directly whether the prp mrna in scrapie brain tissue can encode the scrapie agent, we expressed prp cdna cloned from scrapie-infected mouse brain in vitro. the expressed prp did not transmit scrapie to ...	1989	2574873
prp (proline-rich protein) genes linked to markers es-12 (esterase-12), ea-10 (erythrocyte alloantigen), and loci on distal mouse chromosome 6.	the closely linked proline-rich protein (prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. they are part of a conserved linkage group that is represented on human chromosome 12p. two other markers, ea-10 and es-12, that were previously unassigned to a chromosome are closely linked to prp genes in the mouse.	1989	2613230
bacterial growth and toxin production in ileostomy effluents.	escherichia coli (2), vibrio cholerae (2) and aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. the patterns of response ...	1989	2649680
mutational specificity and the influence of excision repair: insights into the mechanisms of error-avoidance and error-fixation.	the excision repair process controlled by the uvrabc gene in escherichia coli is the major pathway for the repair of a diverse series of dna damages. to achieve a better understanding of the mechanics of this repair pathway and its impact upon mutagenesis, we have applied a recently developed technology by which the nature of mutation is determined at the dna sequence level. a comparison of the classes and distribution of mutation in excision-repair-proficient and excision-repair-deficient strai ...	1989	2698833
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.	in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...	1988	2838023
roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo.	fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ...	1988	3044171
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.	shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...	1988	3045003
stimulation of rabbit synoviocyte prostaglandin e2 synthesis by lipopolysaccharides and their subunit structures.	lipopolysaccharides (lps) induce synoviocyte activation and may lead to destruction of synovial joint tissues. we assessed the production of prostaglandin e2 (pge2) as a measure of synoviocyte activation by lps and their subunit structures. diphosphoryl lipid a was the smallest portion of lipid a tested that stimulated pge2 production. the polysaccharide fraction of lps, containing the o antigen, was also active. intraarticular injections of the polysaccharide resulted in a synovitis very simila ...	1988	3162365
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
biochemical and toxigenic characteristics of aeromonas spp. isolated from diseased mammals, moribund and healthy fish.	in this study we describe biochemical, toxigenic and surface characteristics of 33 motile aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. aeromonas hydrophila, a. caviae and a. sobria were isolated from both mammals and fish but at a different incidence. aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, catt ...	1988	3188376
induction of lyme arthritis in lsh hamsters.	in studies of experimental lyme disease, a major obstacle has been the unavailability of a suitable animal model. we found that irradiated lsh/ss lak hamsters developed arthritis after injection of borrelia burgdorferi in the hind paws. when nonirradiated hamsters were injected in the hind paws with b. burgdorferi, acute transient synovitis was present. a diffuse neutrophilic infiltrate involved the synovia and periarticular structures. the inflammation was associated with edema, hyperemia, and ...	1988	3410540
gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.	clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...	1988	3366862
placental amino acid uptake in normal and complicated pregnancies.	amino acids are the essential substrates for fetal growth and catabolism. the fetus is dependent on the placenta for the provision of amino acids, the first step being concentration of amino acids within the syncytiotrophoblast for subsequent transfer to the fetus. a reliable technique for the isolation of human syncytiotrophoblast plasma membrane has been described, and the suitability of this preparation for the study of amino acid uptake and membrane transport has been well documented. using ...	1988	3354595
[toxic properties of lipopolysaccharide and lipid a of aeromonas sobria and non 01 vibrio cholerae].		1988	3216765
[immunogenic and antigenic properties of bacterial cells, lipopolysaccharides and lipids a of aeromonas sobria and non 01 vibrio cholerae].		1988	3216766
enteropathogenicity of aeromonas species isolated from infants: a cohort study.	the significance of aeromonas spp. as potential enteric pathogens was evaluated in a cohort of 187 infants aged 3-18 months during a 16-week summer period. aeromonas spp. were isolated from 14 of the 196 (7.1%) diarrhoeal episodes detected and from eight (5.2%) of 153 samples from paired asymptomatic infants. carriage of bacterial enteropathogens excluding aeromonas spp. was detected in a high proportion (23%) of the asymptomatic children. almost all of the seven isolates of aeromonas sobria wer ...	1988	3216131
carbon substrate assimilation patterns of clinical and environmental strains of aeromonas hydrophila, aeromonas sobria and aeromonas caviae observed with a micromethod.	the assimilation of carbon substrates by 103 strains of aeromonas of different origin identified by conventional methods was studied by means of a standardized micromethod containing 147 tests (api system). six distinct groups could be recognized and the discriminating substrates were determined. 3 species of aeromonas can be identified by means of conventional method: a. hydrophila, a. sobria and a. caviae. the method has a number of drawbacks: some media are unreliable, others are difficult to ...	1988	3218392
[serological cross reactions of lps and lipid a isolated from aeromonas sobria and non 01 vibrio cholerae].		1988	3246916
aeromonas-associated gastroenteritis in children.	during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...	1988	3340460
evaluation of prostaglandins as mediators of tubuloglomerular feedback.	orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ...	1988	3364575
[characteristics of immunoglobulins specific for lps and lipid a of aeromonas sobria and non 01 vibrio cholerae].		1988	3246917
an unusual case of pyogenic meningitis due to aeromonas sobria.		1988	3382842
biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah.	an epizootic of feline infectious peritonitis in a captive cheetah population during 1982-1983 served to focus attention on the susceptibility of the cheetah (acinoyx jubatus) to infectious disease. subsequent observations based upon seroepidemiological surveys and electron microscopy of fecal material verified that cheetahs were indeed capable of being infected by coronaviruses, which were antigenically related to coronaviruses affecting domestic cats, i.e. feline infectious peritonitis virus/f ...	1988	2849387
nucleotide sequence and transcriptional analysis of the aercaera region of aeromonas sobria encoding aerolysin and its regulatory region.	the nucleotide sequence of a 2510 base pair chromosomal fragment containing the aerolysin gene aera, and its regulatory region aerc, from a clinical isolate of aeromonas sobria was determined. the aerolysin gene coded for a 54.5 kd polypeptide and had a g + c content of 59%, indicating that it is endogenous to the genus aeromonas. in contrast, the aerc region was characterized by its high a + t content (61%) and the presence of a core motif, aataaaa, repeated eight times within 300 base pairs. a ...	1988	2459581
purification of cytotoxic enterotoxin of aeromonas sobria by use of monoclonal antibodies.	cytotoxic enterotoxin of aeromonas sobria was purified by affinity chromatography with monoclonal antibodies. the purified enterotoxin gave a single protein band in polyacrylamide gradient gel electrophoresis and its mol. wt estimated by this technique was 63,000; it had a pi of 6.2. the purified enterotoxin caused fluid accumulation in rat ileal loops and in infant mice, was cytotoxic to cultured cells, was haemolytic to human erythrocytes, and was lethal to mice after intravenous injection. th ...	1987	3104592
aeromonas cytotonic enterotoxin cross reactive with cholera toxin.	isolation by affinity chromatography from crude culture filtrate of aeromonas sobria of protein that cross reacted with cholera toxin (ct) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of y1 adrenal cells. all these activities were neutralised by antiserum to ct. there was no haemolytic or cytotoxic activity associated with this ct-cross reactive cytotonic enterotoxin. ct-cross reactive material detected in enzyme linked immunosorbent ...	1987	3560188
clearance of chlamydial elementary bodies from the conjunctival sac.	the rate of disappearance of inactivated chlamydia trachomatis elementary body (eb) preparations from the conjunctival sac was studied in monkeys. direct fluorescent antibody (dfa) cytology showed that the majority of eb had been cleared from the eye within 24 hr of the inoculation of 1 x 10(6) inactivated eb, although small numbers of eb could be detected for up to 144 hr. the rate of clearance in normal and ocular immune animals did not differ, and formalin-killed and uv-inactivated ebs disapp ...	1987	3298129
role of enterobius vermicularis in the aetiology of appendicitis.	an evaluation was made of the histological material obtained from all 1529 appendices removed during the last 5 years at southmead hospital, bristol, in order to elucidate possible relationships between the incidence of enterobius vermicularis and the origin of inflammation in the appendix. in total, 1419 appendices were removed as cases of clinical appendicitis and 110 in the course of another surgical procedure. e. vermicularis was identified in 2.7 per cent of patients with clinical appendici ...	1987	3651783
virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.	autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ...	1987	3679544
importance of aeromonas sobria in aeromonas bacteremia.		1987	3805781
a practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.	a known ornithine decarboxylase assay working with ion exchange separation of [3h]ornithine and [3h]putrescine has been revised. the assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. the separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. the detection limit is about 50 pmoles [3h]putrescine formed.	1987	3545892
differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor.	motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative.	1987	3611325
plasmids and resistance to antimicrobial agents in aeromonas sobria and aeromonas hydrophila clinical isolates.	the antimicrobial susceptibilities of 75 aeromonas isolates were determined by agar dilution. differences in resistance patterns were observed between strains isolated from different geographic locations and between a. sobria and a. hydrophila isolates. multiple resistance was common; however, only one conjugative plasmid was detected. this 110-megadalton plasmid mediated resistance to eight antibiotics.	1987	3631947
changes in rat adrenal tyrosine hydroxylase mrna levels following chronic amphetamine administration.		1987	2888130
determination of antimony levels in organs of mice, treated with an antimony-albumin complex, by atomic absorption spectroscopy.		1987	3609261
the immediate overlay denture.		1987	269213
[specialization in periodontology].	in addition to the demand that periodontal therapy should be the task of any stomatologist, the considerable morbidity and the aetiopathological special features of periodontal diseases call for specialists. periodontal basic care and specialized therapy easy to delimitate. the specified stomatological curriculum allows for the demands on periodontology; it is imperative to enforce them in education and continuing education.	1986	268701
different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice.	fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ...	1986	362823
scintigraphic study of the gastrointestinal transit of a liquid meal in patients with chronic chagas' disease.	gastric emptying and small bowel transit of a liquid meal (isotonic dextrose) were assessed by a scintigraphic technique in 16 patients with chagas' disease involving the esophagus and/or the colon, including one case with mega-jejunum, and in 10 control subjects. initial gastric emptying was faster in the chagas' disease group than in controls (gastric retention at 15 min, median and (range): 52% (15-86) vs 71% (43-97), p < 0.01) although there was no significant difference in total gastric emp ...	1986	1339510
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.	although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...	1986	3022716
fatal infection with aeromonas sobria and plesiomonas shigelloides.		1986	3081156
prevalence, species differentiation, and toxigenicity of aeromonas strains in cases of childhood gastroenteritis and in controls.	in a 1-year period (january to december 1984), aeromonas strains were isolated from feces of 21 of 561 (3.7%) children with gastroenteritis and from 12 of 576 (2.1%) children without intestinal disturbances (controls). the difference between the two isolation rates was not significant (x2 = 2.2; p greater than 0.05). in five cases of illness other intestinal pathogens were isolated together with aeromonas in the same stool sample. a total of 39 aeromonas strains were detected since in some cases ...	1986	3958147
a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments.	vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ...	1986	3485723
measurement of picogram quantities of rat ige from in vitro culture supernatants of b lymphocytes in experimental helminth infections.	a double-antibody liquid-phase radioimmunoassay was used to measure picogram quantities of rat ige. the sensitivity of this method (approximately 10 pg/ml) allowed the determination of the production of ige in in vitro culture supernatants of b lymphocytes of infected animals.	1986	3488274
incidence and virulence of aeromonas species in feces of children with diarrhea.	aeromonas spp. occurring in feces of children with diarrhea were studied. forty-eight strains were isolated from 2,025 specimens during a one year period. only 11 of 44 strains tested yielded virulence factors (cytotoxin, hemolysin and hemagglutinin). six strains were identified as aeromonas sobria and five as aeromonas hydrophila. the other strains isolated were identified as aeromonas caviae. the biochemical characteristics associated with virulence factors were a positive voges-proskauer reac ...	1986	3743554
production of "asao toxin" by aeromonas strains isolated from feces and drinking water.	cultures of aeromonas species were tested for production of a toxin recently purified by asao et al. (t. asao, y. kinoshita, s. kozaki, t. uemura, and g. sukaguchi, infect. immun. 46:122-127, 1984) and described as a hemolysin with enterotoxic and cytotoxic activity. the toxin was produced by only 63% of aeromonas sobria strains and by 93% of aeromonas hydrophila strains. also, 54% of a. hydrophila strains produced another cytotoxic entity.	1986	3711306
whole-body autoradiography of [3h]dihydrostreptomycin in guinea pigs and rats: the labelling of the inner ear in relation to other tissues.	[3h]dihydrostreptomycin was given intramuscularly to young pigmented guinea pigs and rats. whole-body autoradiography, combined with densitometric measurement of the blackening of the autoradiograms, and liquid scintillation counting were used to determine the levels of radioactivity in the inner ear in relation to blood and other tissues. it was found that there was an accumulation of radioactivity in the perilymph of both the cochlear and vestibular parts of the labyrinth. the labelling of the ...	1986	2422870
[diarrhea caused by aeromonas in ivory coast].	the authors drawn epidemiological, clinical conclusions, and the geographical data concerning the diarrheas with aeromonas in ivory-coast. the results relate to a study realized from january 1982 to july 1985. out of the 1,594 excrements 627 germes have been isolated and among which 153 strains of aeromonas if for instance 24.4% of the whole germs. aeromonas caviae is the most frequent species (50%) compared to 27.3% for aeromonas sobria, and 22.7% for aeromonas hydrophila.	1986	3026671
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.	sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...	1986	3517061
partial characterisation of a soluble haemagglutinin from human diarrhoeal isolates of aeromonas.	a soluble haemagglutinin has been identified in cell-free culture supernates of human diarrhoeal isolates of aeromonas sobria, a. hydrophila and a. caviae. it was oligomeric; a major peak of haemagglutinating activity had an apparent mol. wt of 780,000 but there was haemagglutinating activity throughout the mol. wt range less than 40,000- greater than 10(6). human group o, a and b, horse, rabbit, chicken and rat erythrocytes, but not those of sheep and cow, were agglutinated by the soluble haema ...	1986	3723584
pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni.		1986	3703054
morphological changes in rat striatal boutons after chronic methamphetamine and haloperidol treatment.	dopaminergic (da) synaptic boutons were identified in rat striatum with an electron microscopic histochemical method. in rats which developed behavioral hypersensitivity after treatment with methamphetamine (map) for about 2 weeks, significantly fewer da boutons were found. this effect was specific to da boutons without mitochondria and was not seen in boutons with mitochondria. the density of granular synaptic vesicles in da boutons, however, did not change significantly. these morphological ch ...	1986	3748472
effect of dilauroylphosphatidylcholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs.	incubation of bull sperm with liposomes made with phosphatidylcholine (pc) containing fatty acyl chains of either 10 (pc10) or 12 (pc12) carbons resulted in greater than 90% of the sperm exhibiting an acrosome reaction (ar) within 15 min. liposomes of pc10 rapidly destroyed sperm motility while pc12 acrosome-reacted sperm remained motile for several h. liposomes of pc with greater than or equal to 14-carbon fatty acyl chains had no effect on the ar or motility of sperm. the ar was not induced by ...	1986	3768462
a case report: aeromonas sobria gastroenteritis in an adult.	aeromonas sobria, a member of the aeromonas hydrophila group, recently has been recognized as a gastrointestinal pathogen in adults. there have been a few documented cases, and these have come from far eastern countries, namely india, thailand, and indonesia. the authors have isolated a strain of a. sobria from a 62-year-old male patient suffering from diarrheal disease. he had no history of travel outside of the united states for several decades. he presented with a severe diarrheal disease tha ...	1985	3883745
chromosome 16q, eosinophilia, and leukemia.		1985	3967211
pathogenicity of aeromonas.	the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...	1985	3981022
comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides.	the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides.	1985	4004196
in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents.	mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested.	1985	4037775
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.	sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...	1985	4056003
cephalothin susceptibility as a potential marker for the aeromonas sobria group.	fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.	1985	4056009
[aquatic contamination of wounds by aeromonas sobria after bathing in the river].		1985	3160041
hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes.	the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ...	1985	2866480
identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.	gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0.	1985	3934208
aeromonas caviae: an enteric pathogen?	of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.	1985	4066046
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.	the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...	1985	4044041
the ada conference on increasing access to dental care. a report.		1985	268525
morphological correlates of synaptic transmission in lamprey spinal cord.	the dye procion brown was used to identify in the light and electron microscope, synaptic contacts made between monosynaptically coupled neurons in the lamprey spinal cord whose synaptic interaction had been recorded. synaptic contacts were made on different dendrites of the postsynaptic cell at different distances from the soma. some of the contacts were made on dentritic spines and some on the smooth shaft of the dentrites. serial sections through synaptic contacts made on dendritic processess ...	1985	176330
secondary hypertriglyceridemia and hyperlipoproteinemia in patients with primary asymptomatic gout.	we carefully selected 30 men with primary gout, rendered asymptomatic by therapy, to examine the frequency and type of hyperlipidemia and hyperlipoproteinemia, with the objective of determining whether serum uric acid, alcohol intake, liver function, kidney function, and (or) drugs were participating in the secondary lipid disorder. sixty-one age- and sex-matched men were used as controls. about 73% of the gout patients had hypertriglyceridemia, 1.6-fold the frequency found in the control group. ...	1985	262176
the value of a simple medical questionnaire in dentistry.	a short specific medical questionnaire was given to 1125 new patients attending for their initial examination at the perth dental hospital. a positive history was given by 398 patients. a wide variety of conditions was revealed and the dental significance is discussed. the extent of the information provided illustrates that the simple questionnaire is valuable in exposing conditions which may affect dental treatment.	1984	270975
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.	a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...	1984	6699147
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.	a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...	1984	6690467
enterotoxic activity of aeromonas hydrophila and aeromonas sobria.		1983	6421948
distribution of imvc biogroups of aeromonas hydrophila and aeromonas sobria isolated from human, fish and water.	aeromonas sobria and a.hydrophila were isolated from infected fish in ratio of 3.5:1 during the outbreak of fish infections from december 1982 to february 1983, while isolates from human diarrheic stool was 1 :2. on the basis of imvc reactions 138 isolates of motile aeromonads could be divided into 11 biogroups, with biogroup 4 showing statistically significant association with infections. nine biogroups of aeromonads which were isolated from infected fish reflected that the outbreak was not cau ...	1983	6658502
cholera-like illness due to aeromonas sobria.	a thai woman from bangkok was admitted to a hospital in paris for a cholera-like illness. a culture of her "rice-water" diarrhea was negative for vibrio cholerae and enterotoxigenic escherichia coli but was positive for aeromonas sobria. this strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. the enterotoxin, which was labile at 100 c ...	1982	7054327
aeromonas sobria in chlorinated drinking water supplies.	aeromonas species were recovered from over 27% of 183 chlorinated drinking water samples collected during an 18-month period. sixteen of 20 isolates tested elicited a cytotoxic response by y-1 mouse adrenal cells. none of the strains was either enterotoxigenic by the rabbit ligated ileal loop assay, exhibited piliation, or showed significant mannose resistant adherence to human buccal cells. theaeromonas isolates were further identified to bea. sobria and were resistant to ampicillin and suscept ...	1982	24226050
diabetes mellitus and periodontal disease.		1981	273875
serogrouping of motile aeromonas species isolated from healthy and moribund fish.	a total of 195 strains of motile aeromonas isolated from fish were characterized as aeromonas hydrophila and aeromonas sobria. in view of the frequency of isolation and the importance of motile aeromonas species as fish pathogens, a serological classificaton of these organisms was attempted. antisera were prepared in rabbits against formalinized whole cell suspensions and against boiled cells of 12 different isolates. seventy-six strains could be grouped by tube agglutination with whole cells as ...	1981	7259164
association of aeromonas sobria with human infection.	fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...	1981	6112237
a toxigenic profile of aeromonas hydrophila and aeromonas sobria isolated from fish.	forty strains of motile aeromonas were isolated from healthy and diseased fish. these strains were identified as a. hydrophila or a a. sobria. it was found that only strains of a. hydrophila produced a dermonecrotic factor and two zones of hemolysis on blood agar. all the strains of a. sobria tested and 72% of the a. hydrophila were enterotoxigenic. finally, we observed that only a. hydrophila strains could regularly produce hemolysin at 10 degrees c.	1981	7237279
isolation, enumeration, and characterization of aeromonas from polluted waters encountered in diving operations.	counts of total viable, aerobic, heterotrophic bacteria, indicator organisms, and aeromonas spp. were made at a diver training site on the anacostia river in washington, d.c. the numbers of aeromonas cells in anacostia river sediment and water increased during periods of elevated water temperature, to maxima of 4 x 10(5) cells per g of sediment and 300 cells per ml of water. correspondingly, aeromonas counts dropped 2 to 4 logs as the water temperature decreased to 0 to 0.5 degrees c. cultures t ...	1980	7396482
[bilirubin in the blood serum of clinically healthy cows and those ill with ketosis and hepatopathies].	studied was the bilirubin equilibrium in 62 normal cows, 52 cows with clinical ketosis, 31 cows with diffuse liver injury, and 14 cows that went off food for five days. a moderate rise of the total bilirubin of mixed type was found in ketosis-affected cows. statistically significant proved the changes in the free fraction, however, there were no persuasive data that they were due to superproduction of bilirubin. changes in the total bilirubin similar to those in cows with ketosis were establishe ...	1979	516442
microscopic changes in rat tongue following experimental cryosurgery.	histological changes were studied in the rat tongue for varying periods of time up to 14 days following the application of a cryoprobe to the dorsal surface of rat tongue. within minutes of the application, focal subepithelial vesiculation and hyperemia occurred. at 3 h there was evidence of damage to capillary walls, resulting in hemorrhage. arterioles and venules were thrombosed and there were degenerative changes in the muscle layer of their walls. at 6 h ventral epithelium showed evidence of ...	1979	108379
in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase.	infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ...	1979	85719
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses.		1979	88270
control of v kappa expression in the mouse. i. unexpected expression of the v kappa allele, igk-pcb, in a somatic cell hybrid of akr (igk-pca) origin.		1979	89158