Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| expression of the asba1, oxa-12, and asbm1 beta-lactamases in aeromonas jandaei aer 14 is coordinated by a two-component regulon. | aeromonas jandaei aer 14 (formerly aeromonas sobria aer 14) expresses three inducible beta-lactamases, asba1, oxa-12 (asbb1), and asbm1. mutant strains that constitutively overexpress all three enzyme simultaneously, suggesting that they share a common regulatory pathway, have been isolated. detectable expression of the cloned genes of asba1 and oxa-12 in some escherichia coli k-12 laboratory strains is achieved only in the presence of a blp mutation. these mutations map to the cre operon at 0 m ... | 1997 | 9068648 |
| evaluation of caribbean strains of macrocyclops and mesocyclops (cyclopoida:cyclopidae) as biological control tools for the dengue vector aedes aegypti. | fifteen caribbean strains of copepods were assessed for their predation ability against mosquito larvae. macrocyclops albidus from nariva. mesocyclops aspericornis from oropouche, and mesocyclops longisetus from e1 socorro, trinidad, were most effective against aedes aegypti but not against culex quinquefasciatus. mesocyclops longisetus and me. aspericornis prevented any mosquito survival over 25 wk of observation despite weekly challenges with ae. aegypti. the copepods were tolerant to dosages ... | 1997 | 9152870 |
| aeromonas sobria endophthalmitis. | aeromonas sobria causes a rare gram-negative bacterial water-borne infection. it has been found in waters of north queensland and south-east asia. of all aeromonas species, a. sobria is the most virulent and invasive and has been reported to cause soft tissue infection and corneal ulcer. | 1997 | 9395834 |
| [bacteremia for aeromonas sobria in a patient with cholecystitis]. | 1997 | 9221025 | |
| [severe acute gastroenteritis due to aeromonas in a patient colectomized for crohn's disease]. | we report a case of severe acute diarrhoea produced by aeromonas sobria, a specimen with controversial human pathogenicity. a 26-year-old man with crohn's disease previously colectomized was admitted with a cholera-like clinical presentation and renal failure. aeromonas sp. can be isolated in both children and adults and in healthy people, a. hydrophila being most often detected. severe infections due to other aeromonas sp., usually a. sobria and a. caviae, have been rarely described. most patie ... | 1997 | 9090982 |
| acute rhabdomyolysis and myonecrosis complicating aeromonas bacteremia in neutropenic patients with hematologic malignancies: report of two cases. | infections by aeromonas spp. are a rare cause of systemic infection in normal and immunocompromised hosts. we report the cases of two patients with acute non-lymphoblastic leukemia who developed septic shock by aeromonas species with unusual soft-tissue complications. one patient who was undergoing consolidation chemotherapy developed septic shock by aeromonas hydrophila with rhabdomyolysis and subsequent soft-tissue destruction consistent with myonecrosis. she recovered with combination antibio ... | 1997 | 9499669 |
| evaluation of the synergistic haemolysis (camp-like) test in the identification of motile, mesophilic aeromonas species. | usefulness of the synergistic haemolysis (camp-like) test in the identification of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae was evaluated, using beta-toxin producing staphylococcus aureus atcc 25923 and alpha-toxin producing clostridium perfringens atcc 13124 as sphingomyelinase producing indicator strains. the tests were done on columbia blood agar base supplemented with 5% sheep blood (cba; difco laboratories, detroit, mi, usa). when cultured aerobically, 77% of a. hydrophi ... | 1997 | 9241799 |
| aeromonas bacteremia in patients with hematologic diseases. | over a 23-year period, 17 patients with hematologic diseases developed aeromonas bacteremia while in our hematology ward. male predominance (14 patients, 82%) was seen, with a predilection for the elderly. hematologic malignancies, especially acute leukemia, accounted for 15 (88%) of all patients. cancer chemotherapy and neutropenia (15 patients each) were the most common preceding host conditions. aeromonas bacteremia generally occurred in the second half of the year (july-december), with no ex ... | 1997 | 9144007 |
| aeromonas sobria-associated left-sided segmental colitis. | aeromonas species, once thought pathogenic only in immunocompromised hosts, have more recently been found to be associated with many different infections in previously healthy individuals. the most common site of these infections is the gastrointestinal tract. we describe a 67-yr-old woman who presented with abdominal pain and bloody diarrhea and was diagnosed with left-sided, segmental colitis due to aeromonas sobria, which was proven by stool culture. extensive work-up for ischemic colitis was ... | 1997 | 9362202 |
| biochemical characterization of the carbapenem-hydrolyzing beta-lactamase asbm1 from aeromonas sobria aer 14m: a member of a novel subgroup of metallo-beta-lactamases. | asbm1, a carbapenem-hydrolyzing beta-lactamase produced by aeromonas sobria aer 14m, was purified chromatographically, with anion exchange chromatography performed in the absence of zn2+. the molecular mass of asbm1 was approximately 34,000; the isoelectric point was 9.1. asbm1 had high hydrolytic specificity for carbapenems but low hydrolysis rates for penicillins and cephalosporins. asbm1 was resistant to the commercially available beta-lactamase inhibitors but was inhibited by pcmb and the ch ... | 1996 | 8998985 |
| [rapid detection of the hemolysin genes in aeromonas sobria by the polymerase chain reaction]. | the hemolysin of aeromonas sobria is one of the important virulence factors in this organism. rapid detection and identification test for a. sobria is important for early and specific diagnosis of this infectious disease. we evaluated the polymerase chain reaction (pcr) for the rapid detection of a. sobria. two pairs of synthetic oligonucleotide primers (asa1-s and a; aeraas-s and a) were used in pcr technique to detect the different hemolysin genes (asa1 and aeraas) in a. sobria. the pcr identi ... | 1996 | 9011120 |
| enzyme kinetics and biochemical analysis of imis, the metallo-beta-lactamase from aeromonas sobria 163a. | the metallo-beta-lactamase from aeromonas sobria 163a, imis, was isolated in a two stage purification procedure using protein affinity columns. enzyme kinetics show that imis hydrolyses the carbapenems but displays poor activity against other beta-lactams. imis possesses the narrowest spectrum of activity of the group 3 enzymes that have been analysed. sequencing of the 40 n-terminal amino acids show this region to be identical to that of the cpha metallo-beta-lactamase from aeromonas hydrophila ... | 1996 | 9182099 |
| [endophthalmitis caused by aeromonas sobria]. | 1996 | 9011213 | |
| [bacteriological studies of travellar's diarrhoea. 5) analysis of enteropathogenic bacteria at osaka airport quarantine station from january 1992 through september 3rd, 1994]. | during the last 2 years and 8 months before the closure of osaka airport quarantine station (from jan. 1992 to sep. 3, 1994), a total of 7,421,909 overseas travellers were quarantined. 15,919 reported themselves of suffering from diarrhoea. bacteriological examination of a total of 6,031 individuals' stools were performed. 1) various enteropathogenic bacteria were isolated from 31.2% of the stools examined. isolated species were as follows: plesiomonas shigelloides, 1,127 cases (59.9%); vibrio p ... | 1996 | 8822051 |
| antidiarrhoeal activities of ocimum gratissimum (lamiaceae). | the antidiarrhoeal activities of leaf extracts of ocimum gratissimum were investigated by disc diffusion and tube dilution methods. the extracts were active against aeromonas sobria, escherichia coli, plesiomonas shigelloides, salmonella typhi, and shigella dysenteriae. the leaf extracts were most active against s. dysenteriae and least active against s. typhi. the sensitivity of the organisms measured in terms of zone of inhibition ranged from 8.00 to 19.50 mm. the minimum inhibitory concentrat ... | 1996 | 9203793 |
| bacterial diarrhea in laos, a region where cholera was endemic. | there were cholera outbreaks in lao pdr since 1993. two provinces were epidemic areas of cholera in 1993. however the area of cholera occurrence increased to 7 provinces through 1994. then a bacterial survey had done for the purpose of public health improvement. epec was markedly isolated from the hospital in vientiane. in apparent infection of salmonella and non-01 vibrio cholerae with soldiers stationed in the island in mekong river belong to vientiane were pointed out. the outbreak of diarrhe ... | 1996 | 9253874 |
| prolyl aminopeptidase is also present in enterobacteriaceae: cloning and sequencing of the hafnia alvei enzyme-gene and characterization of the expressed enzyme. | the hafnia alvei prolyl aminopeptidase gene (hpap) was cloned and sequenced, the expressed enzyme (hpap) was purified to homogeneity and thoroughly characterized. an open reading frame of 1,281 bp was found to code for the enzyme, resulting in a protein of 427 amino acids with a molecular weight of 48,577. hpap resembles the aeromonas sobria enzyme, having 45% identity and the same distinctive properties with respect to size and substrate specificities. both enzyme show similar chromatographic b ... | 1996 | 8830041 |
| hlya hemolysin of vibrio cholerae o1 biotype e1 tor. identification of the hemolytic complex and evidence for the formation of anion-selective ion-permeable channels. | hemolysin (hlya) was concentrated from supernatants of different vibrio cholerae o1 biotype e1 tor strains by ammonium sulfate precipitation. the concentration of the toxin in the supernatants and in the precipitates was quantified using its hemolytic activity. the toxin formed a high molecular-mass band (about 220 kda) on sds/page while the toxin monomer had a molecular mass of 60 kda when it was heated. the addition of the e1 tor hemolysin oligomers, but not that of the monomers, to the aqueou ... | 1996 | 8856066 |
| near-drowning-associated aeromonas pneumonia. | aeromonas is increasingly recognized as a human pathogen that causes a variety of different infections. aeromonas has rarely been reported as a cause of respiratory infection, and it has been described in near-drowning-associated pneumonia. this article reviews a case of aeromonas sobria pneumonia associated with a near drowning and considers the clinical and epidemiological characteristics of 10 previously reported cases. nearly all of the cases involved young healthy men, a rapid development o ... | 1996 | 8969997 |
| growth and exotoxin production at low temperatures by aeromonas strains in meat slurries. | the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (haemolysin and enterotoxin) and exoenzymes (protease and lipase), as well as their growth in meat slurries stored at low temperatures (5 and 12 degrees c) was investigated. the results showed that aeromonas grew well in meat slurries stored at low temperatures in the presence of background flora. exotoxins and exoenzymes were not detected in filtrates from meat slurries inoculated with enter ... | 1996 | 8643030 |
| endotoxin in aeromonas sobria infection: speculative pathogenesis and treatment strategies in advanced liver disease with soft-tissue infection. | 1996 | 8678032 | |
| aeromonas sobria infection with severe muscle degeneration in a patient with alcoholic liver cirrhosis. | a 56-yr-old male who had been followed for alcoholic liver disease was admitted for abdominal pain and a high fever. gastrointestinal endoscopy revealed bleeding esophageal varices that were treated by endoscopic sclerotherapy. blood culture on admission was positive for aeromonas sobria. then skin bullas and ulcers and severe muscle degeneration developed. the patient died despite extensive treatment with antibiotics. a. sobria infection in patients with liver cirrhosis is rare. | 1995 | 8540527 |
| studies on bacteriocinogenic lactobacillus isolates from selected nigerian fermented foods. | ten bacteriocin-producing (bacteriocinogenic) lactobacillus isolates obtained from three nigerian fermented foods namely: kenkey, ogi and wara were tested against the following indicator organisms: lactobacillus plantarum and food borne pathogens comprising enterotoxigenic escherichia coli, enterohaemorrhagic escherichia coli, serratia, pseudomonas, vibrio cholerae, aeromonas sobria, aeromonas cavice, salmonella typhimurium, plesiomonas shigelloides and staphylococcus aureus. all the bacteriocin ... | 1995 | 8568643 |
| [phenotype markers in strains of aeromonas isolated in cuba from children with an acute diarrheic disease]. | twenty-seven cases of aeromonas isolated from 300 five-years children with acute diarrheal disease (eda) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. the percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher th ... | 1995 | 9805080 |
| a clinical isolate of aeromonas sobria with three chromosomally mediated inducible beta-lactamases: a cephalosporinase, a penicillinase and a third enzyme, displaying carbapenemase activity. | hydrolytic profiles of aeromonas sobria 163a, non-induced and induced, and of a derepressed mutant 163a-m, indicate that this strain has inducible beta-lactamases with activities against cephalosporins, penicillins and carbapenems. three enzymes were identified and two of the beta-lactamase genes, amps, encoding a penicillinase and ceps, encoding a cephalosporinase, were cloned into escherichia coli, permitting analysis of the individual enzymes. isoelectric focusing (ief) analysis using inhibit ... | 1995 | 7759391 |
| sequence analysis of two chromosomally mediated inducible beta-lactamases from aeromonas sobria, strain 163a, one a class d penicillinase, the other an ampc cephalosporinase. | two beta-lactamase genes from aeromonas sobria, strain 163a, have been cloned and sequenced, one encoding a typical class c cephalosporinase, designated ceps, the other a class d penicillinase, designated amps. ceps is predicted to be a mature protein of 38 kda with a pi value of 7.0. the amino acid sequence of ceps is most similar to that of ampc from pseudomonas aeruginosa (54.7%). amps is predicted to be a mature protein of 27 kda with a pi value of 7.9 that mostly closely resembles blad from ... | 1995 | 8537283 |
| distribution of aeromonas species in the intestinal tracts of river fish. | aeromonas isolates were obtained from fish intestines, water, and sediments from an urban river and identified by the dna-dna microplate hybridization method. the isolates were aeromonas veronii (22%), aeromonas caviae (18%), aeromonas hydrophila (13%), aeromonas sobria (8%), aeromonas jandaei (7%), and other aeromonas spp. (33%). aeromonas species occurred at high densities with high incidences, regardless of season. the results strongly suggest that aeromonads are indigenous in fish intestines ... | 1995 | 16535171 |
| variable expression of o-antigen and the role of lipopolysaccharide as an adhesin in aeromonas sobria. | on initial isolation of aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767o) and translucent (3767t). strain 3767o consistently produced lipopolysaccharide (lps) core and o-antigen side chain, detectable by sds-page and by western blotting with an o-antigen-specific monoclonal antibody. strain 3767t produced lps core but the amount of o-antigen was dependent on factors including growth medium and bacterial growth phase. strain 3767t exhibited sig ... | 1994 | 7525403 |
| cloning and expression of a cloxacillin-hydrolyzing enzyme and a cephalosporinase from aeromonas sobria aer 14m in escherichia coli: requirement for an e. coli chromosomal mutation for efficient expression of the class d enzyme. | two beta-lactamase genes, asba1 and asbb1, encoding asba1 and asbb1, respectively, have been cloned from aeromonas sobria aer 14m into escherichia coli. asba1 was expressed at low but detectable levels in all e. coli laboratory cloning strains tested. asbb1 was expressed well in the e. coli cloning strain dh5 alpha. however, no enzyme activity could be detected from the same clone when placed in e. coli mc1061. ampicillin-resistant mutants of e. coli mc1061 were obtained that expressed high leve ... | 1994 | 7811022 |
| isolation and characterization of the prolyl aminopeptidase gene (pap) from aeromonas sobria: comparison with the bacillus coagulans enzyme. | the aeromonas sobria pap gene encoding prolyl aminopeptidase (pap) was cloned. it consists of 425 codons and encodes a homotetrameric enzyme of 205 kda. the purified enzyme showed an almost absolute specificity for amino-terminal proline. proline and hydroxyproline residues from many peptide and amide substrates could be easily removed, while no activity was detected for substrates having other amino terminals. the enzyme was very similar to that from bacillus coagulans in many aspects, such as ... | 1994 | 7883756 |
| prolyl aminopeptidase is not a sulfhydryl enzyme: identification of the active serine residue by site-directed mutagenesis. | prolyl aminopeptidase (pap) has been classified as a sulfhydryl enzyme on the basis of its high sensitivity to p-chloromercuribenzoate and heavy metals. recently, however, the possibility of pap being instead a serine enzyme has been raised as a result of two observations--the conservation of some residues among the paps hitherto sequenced, and a similarity to some serine hydrolases. this is the first report describing an attempt to identify the active residue by site-directed mutagenesis. the p ... | 1994 | 7896753 |
| aeromonas sobria spontaneous bacterial peritonitis and bacteremia. | 1994 | 8304329 | |
| aeromonas peritonitis. | five new cases of peritonitis caused by aeromonas species are reported, and 29 others described in the literature are reviewed. males predominated (71%), and the mean age was 56.9 years. acquisition was nosocomial in 20% of the cases. all patients except one (3%) had significant underlying diseases; 73% had chronic hepatic disease, 15% had chronic renal failure (treated with chronic ambulatory peritoneal dialysis [capd]), and 9% had an intestinal perforation. symptoms were similar to those of pe ... | 1994 | 8054435 |
| identification of aeromonas species isolated from freshwater fish with the microplate hybridization method. | aeromonas isolates were obtained from the intestinal tracts of six species of cultured freshwater fish and identified on the basis of their genotypic and phenotypic characters. the microplate hybridization method could differentiate type strains of aeromonas species and related bacteria. dna-dna hybridization analysis showed that 65 aeromonad isolates were 72 to 100% related with either aeromonas caviae, aeromonas hydrophila, aeromonas jandaei, aeromonas sobria, or aeromonas veronii. as many as ... | 1994 | 16349363 |
| constant and variable regions in glycoprotein hormone beta subunit sequences: implications for receptor binding specificity. | 1994 | 201763 | |
| distribution of mesophilic aeromonas species in raw and ready-to-eat fish and meat products in switzerland. | a total of 829 poultry, meat, shellfish and fish products commonly consumed in switzerland were qualitatively and quantitatively examined for the presence of mesophilic aeromonas spp. overall, aeromonads occurred in 24.1% of the samples. raw food products were frequently contaminated (e.g. 94.1% in minced meat), with colony counts up to 6.0 x 10(6)/g. some ready-to-eat products had a relatively high percentage of positive samples as well, such as cooked ham in slices (38.2%), mortadella (12.9%), ... | 1993 | 8268055 |
| characterization of mesophilic aeromonas from clinical specimens by computerized analysis of sds-page protein profiles and by enzymatic activity. | mesophilic aeromonas (aeromonas hydrophila, aeromonas sobria, aeromonas caviae) have recently been considered important aetiological agents of human diseases, mainly gastrointestinal infections. although several findings have pointed out the significance of this group of microorganisms as enteric pathogens and suggested the presence of virulence factors, epidemiological and clinical studies are limited by the difficulty of correctly identifying mesophilic aeromonas at the species level. sds-page ... | 1993 | 8264423 |
| the effect of bacteria infection on mean selected body temperature in the common agama, agama agama: a dose-response study. | 1. the fever response was studied in 43 common agamas using a self-pairing experiment in which animals received an intraperitoneal injection of sterile saline and an injection of one of six dosages of dead aeromonas sobria (1 x 10(6), 1 x 10(7), 1 x 10(8), 1 x 10(9), 1 x 10(10), and 1 x 10(11) total organisms). 2. the results demonstrated a significant increase in tb (1.6-3.1 degrees c) above the mean selected body temperature (msbt) of the saline injection animals over a bacteria infection rang ... | 1993 | 8101779 |
| el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens]. | a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ... | 1993 | 7984811 |
| [the identification of aeromonas species isolated in cuba]. | 100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests. | 1993 | 7984818 |
| wound infection due to aeromonas sobria. | 1993 | 8110950 | |
| cloning and characterization of three hemolysin genes from aeromonas salmonicida. | two hemolysin genes (ash3 and ash4) of aeromonas salmonicida strain 17-2 and one (ash1) of a. salmonicida atcc14174 were cloned into the cosmid vector charomid 9-36 in escherichia coli dh1. the overall amino acid sequence of the ash3 was similar to that of the aerolysins of aeromonas hydrophila and aeromonas sobria, and hemolysins ahh3, ahh4, and ahh5 of a. hydrophila, and hemolysin asa1 of a. sobria. the sequence of ash4 was similar to that of the ahh1 hemolysin of a. hydrophila. the ash4 hemol ... | 1993 | 8309354 |
| [acute gastroenteritis from aeromonas sobria and pancreatitis: apropos of a case]. | 1993 | 8332027 | |
| inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish. | the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ... | 1993 | 8357253 |
| isolation and purification of aeromonas sobria cytotonic enterotoxin and beta-haemolysin. | aeromonas sp., grown in tryptone soya broth supplemented with yeast extract, 0.6%, ph 7.5, and incubated with agitation at 100 oscillations/min for 15 h at 37 degrees c produced optimal amounts of beta-haemolysin and cytotonic enterotoxin. more prolonged incubation resulted in the loss of enterotoxic activity and anion exchange chromatographic analysis indicated the presence of a moiety capable of breaking down the toxin. anion exchange fast protein liquid chromatography resulted in a single pea ... | 1993 | 8384263 |
| spectrum of extraintestinal disease due to aeromonas species in tropical queensland, australia. | during a 12-month period, the clinical spectrum of extraintestinal disease due to aeromonas species was determined for 56 patients in tropical queensland (australia). forty-six patients acquired their infection in the community, six patients were infected in the hospital, and four patients were colonized. demographic risk factors included male gender (67%) and aboriginal ethnic background (35%). the disease ranged from deep-seated infection (four cases) to soft-tissue infection of varying intens ... | 1993 | 8513068 |
| [camp factor for the differentiation among aeromonas species]. | the camp factor technique, described by natale figura as a presumptive method for the differentiation of the species hydrophila, sobria and caviae, was applied to 80 aeromonas strains isolated from children under 5 years with acute diarrheic disease. the typical phenomenon was seen in aerobiosis and anaerobiosis conditions in 10 strains, which were classified as aeromonas hydrophila. in aerobiosis conditions/alone, it was observed in 20 strains, which were identified as aeromonas sobria. it was ... | 1992 | 9768204 |
| characterization of aeromonas sobria tap13 pili: a possible new colonization factor. | pili of aeromonas sobria tap13 were purified and characterized. the molecular mass of the pilin was estimated to be about 23 kda by sds-page. the tap13 pili were immunologically different from a. sobria ae1 pili and a. hydrophila ae6 w pili as previously reported, nevertheless all three had indistinguishable morphology and shared a high degree of homology in their n-terminal amino acid sequences. strain tap13 and its purified pili did not agglutinate human, rabbit or sheep erythrocytes. however, ... | 1992 | 1357078 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| molecular studies on the aerolysin gene of aeromonas species and discovery of a species-specific probe for aeromonas trota species nova. | a large group of aeromonads and other enteric microorganisms were assayed for the presence of the aerolysin gene with use of dna-dna hybridization. two dna fragments corresponding to the regulatory region (aerc) and the structural gene (aera) were used as probes for the detection of the aerolysin gene in these strains. sequences corresponding to the aerolysin structural gene were widespread among aeromonas isolates. in contrast, the aerc probe was much more selective, and sequences corresponding ... | 1992 | 1600007 |
| binding of laminin and fibronectin by the trypsin-resistant major structural domain of the crystalline virulence surface array protein of aeromonas salmonicida. | the surface of aeromonas salmonicida is covered by a tetragonal paracrystalline array (a-layer) composed of a single protein (a-protein, mr = 50,778). this array is a virulence factor. cells containing a-layer and isolated a-layer sheets specifically bound laminin and fibronectin with high affinity. binding by cells was inactivated by selective removal of a-layer at ph 2.2, and neither isogenic a-layer-deficient a. salmonicida mutants nor tetragonal paracrystalline array producing aeromonas hydr ... | 1992 | 1730607 |
| application of ribotyping for differentiating aeromonads isolated from clinical and environmental sources. | we have investigated the usefulness of ribotyping for the differentiation of aeromonads isolated from five patients with gastroenteritis and from the source water, treatment plant, and distribution system of a small public water supply. aeromonas hydrophila and aeromonas caviae were isolated from fecal specimens preserved in cary-blair transport medium by using blood ampicillin agar or alkaline peptone water (ph 8.4) subcultured to blood ampicillin agar plates. a. hydrophila, aeromonas sobria, a ... | 1992 | 1622269 |
| rapid detection of members of the family enterobacteriaceae by a monoclonal antibody. | six monoclonal antibodies directed against enterobacteria were produced and characterized. the specificity of one of these antibodies (cx9/15; immunoglobulin g2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. all of the enterobacteria were specifically recognized, the only exception being erwinia chrysanthemi (one strain tested). bacteria not belonging to members of the family enterobacteriaceae were not detected, except for ... | 1992 | 1622220 |
| [the occurrence of aeromonads in a drinking water supply system]. | this study concerns with the occurrence of aeromonads, coliforms and colony counts in a drinking water supply. aeromonas contents were detected in the range of 15.0 to greater than 2,400/100 ml in the raw water samples of the man made lake. after the drinking water treatment process including fast sand filtration and chlorination aeromonads indicated in comparison to total coliforms and colony counts early and significant an after-growth of maximal 240 aeromonads/100 ml in the peripheric drinkin ... | 1992 | 1609555 |
| enterotoxic effects of aeromonas sobria haemolysin in a rat jejunal perfusion system identified by specific neutralization with a monoclonal antibody. | investigations into the pathogenesis of aeromonas diarrhoea have demonstrated that several different cell-free products of motile aeromonads show enterotoxic activity in suckling mouse, rat, and rabbit assay systems. the relative contributions made by separate cytotoxic and cytotonic activities in the mixture produced by in vitro culture remains unresolved. using a modified rat jejunal perfusion assay, we have studied the effects of a. sobria culture filtrates containing defined levels of haemol ... | 1992 | 1564437 |
| [late onset serum iga and igm deficiency revealed by acute diarrhea caused by aeromonas sobria infection]. | 1992 | 1526403 | |
| aeromonas sobria spontaneous bacterial peritonitis. | 1992 | 1449170 | |
| purification and characterization of aeromonas sobria ae24 pili: a possible new colonization factor. | pili of aeromonas sobria ae24 were purified and characterized. the molecular mass of the pilin was estimated to be about 19 kda by sds-page. the ae24 pili were electrophoretically distinguishable from previously reported aeromonas hydrophila ae6 w pili and a. sobria ae1 pili, although all three had indistinguishable morphology and shared a high degree of homology in the n-terminal amino acid sequences. strain ae24 and its purified pili adhered to rabbit intestine and agglutinated human and rabbi ... | 1992 | 1363704 |
| nucleotide sequences and characterization of haemolysin genes from aeromonas hydrophila and aeromonas sobria. | extracellular haemolysin is thought to be one of the important virulence factors in aeromonas infection. two extracellular haemolysin genes (ahh3 and ahh4) from aeromonas hydrophila strain 28sa, one (ahh5) from a. hydrophila strain ah-1 and one (asa1) from aeromonas sobria strain 33 were cloned into cosmid and plasmid vector dna in escherichia coli. the nucleotide sequences of the open reading frames of ahh3 and ahh4 are both 1476 basepairs (bp), whereas ahh5 and asa1 are 1455 and 1467 bp in len ... | 1992 | 1302284 |
| a positive fever response in agama agama and sceloporus orcutti (reptilia: agamidae and iguanidae). | three species of lizards (agama agama, mabuya perrotetii, and sceloporus orcutti) were tested for a possible increase in mean selected body temperature (msbt) in response to intraperitoneal injection of alcohol-killed aeromonas sobria, a gram-negative bacterium known to be pathogenic to reptiles. a paired experimental design was utilized in which each animal was given an injection of sterile saline and 1.10(10) a. sobria. body temperatures were monitored via indwelling cloacal thermocouples at 4 ... | 1991 | 1939744 |
| gas-liquid chromatographic analysis of cellular fatty acid methyl esters in aeromonas species. | the cellular fatty acids of 39 strains belonging to the genus aeromonas (aeromonas hydrophila, aeromonas caviae, aeromonas sobria, aeromonas media, aeromonas schubertii, aeromonas veronii) were determined by high resolution gas-liquid chromatography. the fatty acid profiles were characterized by major amounts (60% or more) of one saturated (hexadecanoic acid = 16:0) and two unsaturated (hexadecenoic acid = 16:1 and octadecenoic acid = 18:1) acids. while the majority of the strains of the six spe ... | 1991 | 1930556 |
| [severe occurrence of idiopathic ulcerative colitis caused by aeromonas sobria]. | 1991 | 1910924 | |
| isolation and characterization of a cryptic plasmid from mesophilic aeromonads: potential as a cloning vector. | a 2.6 kb covalently closed circular plasmid has been isolated from clinical and environmental isolates of aeromonas sobria and a. hydrophila. the possibility that the plasmid carries genetic determinants that mediate resistance to a variety of anti-microbial agents has been eliminated. the plasmid is stable at approximately 20-25 copies per chromosome equivalent which, together with its relatively small size and the presence of unique restriction sites, makes it a good candidate for development ... | 1991 | 1366999 |
| structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6. | we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ... | 1991 | 1828805 |
| growth of and toxin production by aeromonas hydrophila and aeromonas sobria at low temperatures. | the effects of different temperatures on the growth and toxin production of aeromonas hydrophila and aeromonas sobria were studied. the results showed that these aeromonas species are not only able to grow at low temperatures (e.g. at 4 and 10 degrees c) but may also produce cytotoxin, hemolysin and enterotoxin under suitable growth conditions. | 1991 | 1888660 |
| aeromonas hydrophila: variability in biochemical characteristics of environmental isolates. | a total of 161 strains, including 144 aeromonads, of which 100 had been isolated from water and sediment samples from the chesapeake bay, five clinical and 33 anacostia river isolates of aeromonas hydrophila, six aeromonas sobria, and ten reference cultures were examined in a numerical taxonomy study in which each of the strains were examined for 56 unit characters. similarity between strains was computed using the simple matching (ssm) and jaccard (sj) coefficients. the strains were clustered b ... | 1991 | 1920081 |
| intestinal secretory immune response to infection with aeromonas species and plesiomonas shigelloides among students from the united states in mexico. | intestinal secretory iga (siga) response or lack of response among adults in mexico with diarrhea was used as an indicator of enteropathogenicity of aeromonas species and plesiomonas shigelloides. siga was extracted from stool specimens obtained at day of presentation and 5 days later. total siga was standardized, and specific siga titer against the organism being shed by each patient was determined. western blotting was used to determine which microbial antigens elicited an intestinal siga resp ... | 1991 | 1940478 |
| biochemical and genetic characterization of autoagglutinating phenotypes of aeromonas species associated with invasive and noninvasive disease. | the genetic characteristics and biochemical and structural properties of a number of autoagglutinating (aa) strains of aeromonas associated with invasive and noninvasive disease in humans and infections in animals and from environmental sources were investigated. of 27 strains analyzed by multilocus enzyme typing and dna hybridization studies, 25 (93%) were confirmed to belong to either hybridization group 1 (phenospecies and genospecies aeromonas hydrophila) or 8 (phenospecies aeromonas sobria; ... | 1991 | 2010642 |
| experimental evidence for toxin production by aeromonas hydrophila and aeromonas sobria in a meat extract at low temperatures. | the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (enterotoxin and haemolysin) in a meat extract at low temperatures (5 and 12 degrees c) was investigated. all three strains incubated at 12 degrees c were enterotoxigenic and haemolytic in the meat extract after 5 days. at 5 degrees c, five of the six strains tested were able to produce these exotoxins after 8 days incubation while one strain was neither enterotoxigenic nor haemolytic after 5 ... | 1991 | 2049284 |
| evaluation of the antimicrobial activity of methylglyoxal bis(guanylhydrazone) analogues, the inhibitors for polyamine biosynthetic pathway. | metabolic and antiproliferative effects of methylglyoxal bis(butylamidinohydrazone) (mgbb) and methylglyoxal bis(cyclopentylamidinohydrazone) (mgbcp), inhibitors for polyamine biosynthetic pathway, on escherichia coli, shigella sonnei, aeromonas sobria, aeromonas hydrophila and vibrio cholerae were investigated. mgbb at the concentration of 100 mumol/l depleted intracellular putrescine and spermidine concentrations of e. coli to 25 and 20% of the controls, respectively, while mgbcp depressed the ... | 1991 | 2055790 |
| haemolysin occurrence among aeromonas hydrophila, aeromonas caviae and aeromonas sobria strains isolated from different aquatic ecosystems. | a total of 909 aeromonas spp. isolates from different aquatic ecosystems were tested for haemolysin production by both sheep and horse blood agar-plate assays and by rabbit erythrocytes in broth assay. a comparison of these different methods was undertaken in order to appreciate their capacity to evaluate the haemolytic activity of aeromonas spp. isolated from aquatic ecosystems. the haemolytic activity was associated particularly with a. hydrophila and a. sobria (about 95% of strains), whereas ... | 1991 | 2068383 |
| purification and characterization of aeromonas sobria pili, a possible colonization factor. | pili of aeromonas sobria ae1 were purified and characterized. the molecular mass of the pilin was estimated to be about 23 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the ae1 pili were electrophoretically and immunologically distinguishable from the w pili of a. hydrophila ae6, although the two pili were morphologically indistinguishable. the n-terminal amino acid sequences of the two pilins were identical in the first 10 residues. strain ae1 and its purified pili adhered t ... | 1991 | 1680105 |
| aeromonas spp. and their association with human diarrheal disease. | between january 1988 and december 1989 aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. no other bacterial enteric pathogens were found in any of these 45 patients. of the 45 aeromonas isolates, 35 strains (77.8%) were aeromonas hydrophila, 7 (15.5%) were aeromonas sobria, and 3 (6.7%) were aeromonas caviae. most of the patients were under 5 years of age. no bacterial enteric pathogens, including aeromonas species, were isolated from 512 age- and sex-m ... | 1991 | 2056050 |
| dermoid cyst of the floor of the mouth in an infant. (case report). | dermoid cysts of the floor of the mouth in the infant are rarely encountered although the aetiology is mainly embryological. meyer proposes the classification of the cysts based on the histological differentiation of the elements of the cyst wall. a case is reported which shows differences in the epithelial lining of the cysts found followed by the differential diagnosis and treatment. | 1991 | 269895 |
| [effect of anaprilin on the clinical course of stenocardia and the carbohydrate metabolic indices of ischemic heart disease patients in relation to the nature of their glucose tolerance]. | fourteen-day anaprilin treatment of coronary patients with varying glucose tolerance has demonstrated that the drug's therapeutic efficiency was greater in patients with normal glucose tolerance. anaprilin caused a moderate drop in baseline glucose level and considerably limited the degree of hyperglycemia in response to the glucose tolerance test in coronary patients with abnormally low glucose tolerance. insulin content was virtually unaffected by the drug, irrespective of the type of glucose ... | 1991 | 3327995 |
| primary papillary carcinoma of the prostate: report of a histopathologic, cytologic and electron microscopic study on one case. | an unusual type of prostatic carcinoma is described. histopathologic examination showed a papillary and acinar appearance. aspiration cytology showed papillary fragments and sheets of malignant cells. the ultrastructural features of this tumor were similar to those reported for prostatic carcinoma rather than endometrial carcinoma supporting a prostatic orgin for this tumor. | 1990 | 269605 |
| hemoglobin e and glucose-6-phosphate deficiency in the khmer air force (cambodia). | this study was designed to investigate the use of primaquine in malaria control in the khmer republic (cambodia). blood was drawn from 106 male khmer air force troops for analysis of hemoglobin e and glucose-6-phosphate (g-6-pd) deficiency. a test group of 15 men with g-6-pd deficiency and a group of 31 normal controls were given 15 mg of primaquine each morning for 14 days. the patients were followed for hemoglobinuria and changes in hematocrit. primaquine induced a significant, but not a dange ... | 1990 | 268155 |
| financial management of department of nursing services. | 1990 | 258786 | |
| reflection on geriatrics. | 1990 | 267819 | |
| the incidence of virulence factors in mesophilic aeromonas species isolated from farm animals and their environment. | sixty-one isolates of aeromonas spp. from the faeces of pigs, cows and a variety of associated environmental sources were examined for the characteristics that are reputed to have roles in pathogenicity. most isolates of aeromonas hydrophila were cytotoxic (96.4%) and were capable of producing cell elongation factor (75%) and haemagglutinins (67.9%). in contrast few of the aeromonas caviae isolates produced these three markers (13.6%, 27.3% and 36.4% respectively). in general, aeromonas sobria o ... | 1990 | 2209733 |
| cytostatic and cytotoxic effects of recombinant tumor necrosis factor-alpha on sensitive human melanoma cells in vitro may result in selection of cells with enhanced markers of malignancy. | monolayer cultures of the human melanoma cell lines stml-12, stml-11, stml-14 (third, respectively, twenty-fifth subculture), and skmel-28 derived from specimens representing different stages of tumor progression were treated with 10-10,000 u/ml rtnf-alpha applied for 72 h. the effects of rtnf-alpha on cell proliferation, dna synthesis, cell viability, cloning efficiency, cell division, cell morphology, and the immunophenotype were studied in triplicate experiments. the cell line stml-14(3) reve ... | 1990 | 2258639 |
| spinal cord repair: is tissue oxygenation an important variable? | we have demonstrated the reliability and feasibility of making pto2 recordings from graft and host tissue in the injured spinal cord. the data suggest that the oxygen microenvironment of developing graft and host spinal tissue is clearly different from that found in normal spinal tissue or in transplants that have not survived or integrated well. these same constraints seem to apply to cavitation in developing grafts and poorly developed graft/host interfaces. the similarity between these findin ... | 1990 | 2096642 |
| [new enteropathogens: aeromonas spp]. | aeromonas is one of the type of enteric pathogens which is commonly present in less developed countries. it leads to acute diarrhea in children and traveller's diarrhea in adults. additionally it is also responsible from the extraintestinal infections. aeromonas hydrophila is a bacteria which is isolated from water, soil, foods and rarely from intestinal tract of human. aeromonas sobria is especially isolated from wound infection of scuba diver's. there are other types of aeromonas such as salmo ... | 1990 | 2089235 |
| analysis of the autoagglutination phenomenon, serogroup and hydrophobicity as virulence markers in mesophilic aeromonads isolated from stool cultures. | the autoagglutination phenomenon (aa) was studied in 71 strains of mesophilic aeromonads isolated from stool cultures of patients with acute diarrhea (28 aeromonas hydrophila, 20 aeromonas sobria, and 23 aeromonas caviae). two phenotypes were considered in the aa+ strains: sp+ (self-pelleting) and pab+ (precipitation after boiling). serogrouping of the strains was performed only with the 0:11 serum. other properties studied were agglutination with acriflavine (acr) and crystal violet uptake (cvb ... | 1990 | 2073902 |
| cytostatic and cytotoxic effects of recombinant tumor necrosis factor-alpha on sensitive human melanoma cells in vitro may result in selection of cells with enhanced markers of malignancy. | monolayer cultures of the human melanoma cell lines stml-12, stml-11, stml-14 (third, respectively, twenty-fifth subculture), and skmel-28 derived from specimens representing different stages of tumor progression were treated with 10-10,000 u/ml rtnf-alpha applied for 72 h. the effects of rtnf-alpha on cell proliferation, dna synthesis, cell viability, cloning efficiency, cell division, cell morphology, and the immunophenotype were studied in triplicate experiments. the cell line stml-14(3) reve ... | 1990 | 2258639 |
| assay of all-trans----11-cis-retinoid isomerase activity in bovine retinal pigment epithelium. | 1990 | 2292965 | |
| aeromonas sobria in bubaline (water buffalo) abortion: growth requirement, biochemical characters, antibiotic susceptibility, experimental pathogenicity and serology. | a. sobria was isolated unexpectedly on campylobacter selective medium from abortion specimens of 16 buffaloes. all strains (as1-as16) required campylobacter growth supplement (sodium pyruvate, sodium metabisulphate and ferrous sulphate) and 10% c02 atmosphere on primary isolation. they were unusually sensitive to bile salt and failed to grow on macconkey's agar. biochemically, these strains were homogeneous but antibiogram profile and physiological/other characters revealed a moderate heterogene ... | 1990 | 2351419 |
| isolation of aeromonas spp. from canal water. | aeromonas sobria was identified from soil and canal water at the east end of london. there was a correlation between water and faecal isolates from children attending the nearby children's hospital. in view of the potential risk of enteric and non-enteric infections due to aeromonas spp, efforts are needed to ensure that canals, rivers, natural water sources and domestic water supply are free of these organisms. | 1990 | 2361702 |
| mechanisms of inhibition of various cellular dna and rna polymerases by several flavonoids. | four flavonoids (i.e., baicalein, quercetin, quercetagetin, and myricetin), known to be inhibitors of hiv-reverse transcriptase, have been shown to be more or less inhibitory to the activities of various cellular dna and rna polymerases. the degree of the inhibition varied depending on the combination of the flavonoid and the enzyme species: baicalein was moderately inhibitory to dna polymerase gamma and e. coli dna polymerase i; quercetin was strongly inhibitory to dna polymerase beta and e. co ... | 1990 | 2292590 |
| in vitro susceptibility of aeromonas caviae, aeromonas hydrophila and aeromonas sobria to fifteen antibacterial agents. | in vitro testing of the activity of 15 antibacterial agents against 522 clinical isolates of aeromonas species demonstrated some species-associated trends. amoxicillin plus clavulanic acid was effective against approximately 45% of aeromonas caviae and aeromonas hydrophila, but all aeromonas sobria isolates were resistant. aztreonam, piperacillin and mezlocillin were highly active against all the strains of aeromonas tested. ticarcillin was equally effective against aeromonas caviae and aeromona ... | 1990 | 2387294 |
| dynamics of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae in a sewage treatment pond. | the spatiotemporal dynamics of aeromonas spp. and fecal coliforms in the sewage treatment ponds of an urban wastewater center were studied after 20 months of sampling from five stations in these ponds. isolation and identification of 247 aeromonas strains were undertaken over four seasons at the inflow and outflow of this pond system. the hemolytic activity of these strains was determined. the aeromonas spp. and the fecal coliform distributions showed seasonal cycles, the amplitude of which incr ... | 1990 | 2389929 |
| [spectroscopy of the liver in vivo using nuclear magnetic resonance. a new approach in hepatic physiopathology]. | nuclear magnetic resonance (nmr) spectroscopy is a non-invasive means of detecting numerous compounds, thus enabling real-time studies to be carried out on such topics as energy metabolism, intracellular ph and main intermediate metabolism pathways. this method has been used for several years to study perfused livers and rat livers in vivo, but its application to man has just begun. we describe some of its principal uses, with emphasis on the possibilities it offers to investigate the metabolic ... | 1990 | 2140172 |
| effect of bacterial pyrogen on three lizard species. | 1. three lizard species (callopistes maculatus, gerrhosaurus major, and varanus exanthematicus) were tested for their response to intraperitoneal injection of alcohol-killed aeromonas sobria. 2. a paired experimental design, in which each animal received an injection of sterile saline and 1 x 10(10) a. sobria, was utilized. 3. c. maculatus demonstrated a statistically significant increase in mean selected body temperature (msbt) after bacteria injection. 4. g. major and v. exanthematicus did not ... | 1990 | 1976479 |
| pathogenicity factors and virulence for rainbow trout (salmo gairdneri) of motile aeromonas spp. isolated from a river. | ninety-seven motile aeromonas strains were isolated over a period of a year from samples of water and sediment collected at different sites along a river. strains were regularly recovered from all samples, regardless of the source of isolation or seasonal conditions. isolates were biochemically characterized by the api 20ne system (analytab products, plainview, n.y.) and classified as aeromonas hydrophila (74 strains), aeromonas sobria (11 strains), and aeromonas caviae (12 strains). despite the ... | 1990 | 2312678 |
| pyrazinamidase activity as a phenotypic marker for several aeromonas spp. isolated from clinical specimens. | negative pyrazinamidase activity was significantly associated with aeromonas sobria, and positive pyrazinamidase activity was associated with a. hydrophila and a. caviae (chi 2, p less than 0.0001). the absence of pyrazinamidase activity may be a potentially significant phenotypic marker for a. sobria. | 1990 | 2312687 |
| aerolysin of aeromonas sobria: evidence for formation of ion-permeable channels and comparison with alpha-toxin of staphylococcus aureus. | aerolysin from aeromonas sobria ab3 was isolated and purified. the pure toxin formed sodium dodecyl sulfate-insoluble oligomers in a lipidic environment. the addition of aerolysin to the aqueous phase bathing lipid bilayer membranes resulted in the formation of ion-permeable channels which had a single-channel conductance of about 70 ps in 0.1 m kcl. this defines the toxin as a channel-forming component similar to other toxins but without any indication for an association-dissociation reaction, ... | 1990 | 1694819 |
| rapid presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity. | a new test combination for presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity in conjunction with esculin hydrolysis and gas production from glucose was evaluated. of 40 clinical isolates of aeromonas tested statically at 30 degrees c, seven were identified as aeromonas hydrophilia (non-suicidal, aerogenic, esculin positive), eight as aeromonas sobria (suicide variable, mostly aerogenic, esculin negative), and 25 as aeromonas caviae (suicidal, anaer ... | 1990 | 2209629 |
| electrophoretic analysis of the surface components of autoagglutinating surface array protein-positive and surface array protein-negative aeromonas hydrophila and aeromonas sobria. | the protein and lipopolysaccharide (lps) compositions of 10 autoagglutinating aeromonas hydrophila and aeromonas sobria strains were studied; one group consisted of five serogroup o:11 strains that contained an s layer, while a second group was composed of diverse serogroups that were s layer negative by transmission electron microscopy. all serogroup o:11 strains were found to contain a predominant 52,000- to 54,000-molecular-weight protein that was present on both whole-cell and outer membrane ... | 1990 | 2229347 |
| induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis. | 1990 | 2164982 | |
| direct selection of monoclonal antibodies neutralising the cytotoxic activity of aeromonas sobria. | monoclonal antibodies directed against the cytotoxic activity of aeromonas sobria were raised by immunising mice with a culture supernatant concentrated by ammonium sulphate precipitation. neutralising antibodies were specifically selected for by exposing hybridomas to cytotoxic levels of the immunising preparation. cultures free from cytopathic effects after three hours were selected for further investigation. ten cytotoxin resistant hybridomas were isolated but only two of these produced detec ... | 1990 | 2272497 |