Publications

placental and intestinal alkaline phosphatases are receptors for aeromonas sobria hemolysin.	the aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. we previously identified the putative receptor for a. sobria hemolysin as a p66 protein on intestine 407 cells (microb. pathog. 27 (1999) 215-221). here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (plap). recombinant plap expressed in 293t cells was also found to bind to hemolysin and the binding was ...	2005	15715171
antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish.	motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ...	2005	15747443
aeromonas sobria, a causative agent of disease in farmed perch, perca fluviatilis l.	significant numbers of perch, perca fluviatilis, raised on a pilot fish farm in switzerland presented focal skin lesions on the lateral sides and fin rot. mortality rates reached levels of up to 1% of the total fish on the farm per day. virtually pure cultures of aeromonas sobria were isolated from the liver, kidney, spleen and skin lesions of affected fish. aeromonas sobria isolated from the farmed perch had a haemolytic effect on sheep and trout erythrocytes, autoaggregated, was cytotoxic for ...	2005	15752274
a pore-forming toxin produced by aeromonas sobria activates ca2+ dependent cl- secretion.	bacteria produce many types of hemolysin that induce diarrhea by mechanisms that are not completely understood. aeromonas sobria hemolysin (ash) is a major virulence factor produced by a. sobria, a human pathogen that causes diarrhea. since epithelial cells in the intestine are the primary targets of hemolysin, we investigated the effects of ash on ion transport in human colonic epithelial (caco-2) cells. ash increased short-circuit currents (isc) in a dose-dependent manner, and it also activate ...	2005	15797812
adaptational changes in cellular fatty acid branching and unsaturation of aeromonas species as a response to growth temperature and salinity.	the effects of growth temperature and salinity on the cellular fatty acids were investigated on aeromonas caviae, aeromonas hydrophila and aeromonas sobria. under optimal growth conditions, fatty acids patterns were dominated by even-numbered chains c(16:0), c(16:1cis9), c(18:1cis11), c(12:0) and c(14:0). growth temperature modifications induced, in the three aeromonas species, important changes in fatty acid (i) unsaturation, (ii) branching and (iii) chain length. an important decrease in the c ...	2005	15925007
[detection of multiresistance aeromonas with tem type resistant genes in patients with cirrhosis].	to study the status of beta-lactamase produced by multiresistant aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading.	2005	16201471
recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.	cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ...	2005	16269764
use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum).	from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ...	2005	16336470
[purification and immobilization of chondroitinase from aeromonas sobria yh 311].	chondroitinase has been used as an important tool in the study of the structure, function and distribution of glycosaminoglycans for many years. recently, the enzyme has been reported to be a potential enzyme for chemonucleolysis, an established treatment for intervertebral disc protrasion. in this paper, a chondroitinase had been purified from the culture supernatant of aeromonas sobria yh311 using a simple purification procedure of ammonium sulfate precipitation, qae-sephadex a50 ion exchange ...	2004	15968993
microbial transformation of pyrethroid insecticides in aqueous and sediment phases.	recent studies showed that synthetic pyrethroids (sps) can move via surface runoff into aquatic systems. fifty-six of sp-degrading bacteria strains were isolated from contaminated sediments, of which six were evaluated for their ability to transform bifenthrin and permethrin in the aqueous phase and bifenthrin in the sediment phase. in the aqueous phase, bifenthrin was rapidly degraded by strains of stenotrophomonas acidaminiphila, and the half-life (t1/2) was reduced from >700 h to 30 to 131 h. ...	2004	14768859
universal primer pcr with dgge for rapid detection of bacterial pathogens.	a universal primer pcr (uppcr) combined with denaturing gradient gel electrophoresis (dgge) was evaluated as a method permitting the rapid detection of pathogens. the results show that this method is efficient at amplifying the conserved regions of bacterial 16s rrna genes with universal primers and can detect causative bacterial pathogens rapidly. six species of bacteria from fisheries (pseudomonas fluorescens, vibrio anguillarum, aeromonas hydrophila, vibrio fluvialis, providencia rettgeri and ...	2004	15134888
rapid screening of highly efficient vaccine candidates by immunoproteomics.	diseases caused by microorganisms can be controlled by vaccines, which require neutralizing antigens. therefore, it is very important to identify highly efficient immunogens for immune prevention. by combining immunoproteomics and bacterial challenge after immunization, we developed a rapid method for screening protected antigens of pathogenic bacteria in aquaculture. our approach may be divided into three consecutive steps. first, dominant immunogens of outer membrane proteins are screened by i ...	2004	15378699
an outbreak of hospital acquired diarrhea due to aeromonas sobria.	six children admitted in a 18 bedded hematology-oncology unit, developed acute diarrhea in a four week period between march and april 2001. salmonella senftenberg was the additional pathogen in the stool sample of one patient who developed cola colored urine and pneumonia in the course of his illness. all the aeromonas strains had a similar biotype and antibiogram. the diarrhea subsided spontaneously in two children whilst three responded to antimicrobial therapy. one patient sought discharge an ...	2004	15475633
[ultrasonic localization for closed renal biopsy].		2004	520913
fred jenner hodges, ii, m.d. 1895--1977.		2004	375293
effect of high-energy feedings on low birthweight infants.	a comparison of the effect of 100.5 kj/30 ml (24 kcal/30 ml) feedings with that of 84 kj/30 ml (20 kcal/30 ml) feedings on infants with a birthweight of 1500 g or less was made. no consistent benefits in growth occurred and there was a tendency for an increase in complications in infants fed the 100.5 kj/30 ml milk. hence the higher-energy feeding cannot be recommended as the routine feeding for low birthweight infants.	2004	470692
homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen.	the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ...	2004	387578
[ivan andreevich spiriukhov (on his 70th birthday)].		2003	150266
dl-2-keto-4-hydroxyglutarate-1.		2003	165381
purification of cytochrome b from neurospora crassa and other sources.		2003	152379
resection of a massive arteriovenous malformation of the head and neck.	we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.	2003	154679
[50 years of the institute for experimental pathology and therapy in sukhumi].		2003	148825
aeromonas sobria corneal ulcer.		2003	12579184
solar inactivation of mesophilic aeromonas by exogenous photooxidation in high-rate algal pond treating waste water.	investigations were carried out to observe the effect of sunlight on the survival of mesophilic aeromonas (a. caviae, a. hydrophila and a. sobria) in high-rate algal pond.	2003	12588563
studies of the mechanism of action of the aerolysin-like hemolysin of aeromonas sobria in stimulating t84 cells to produce cyclic amp.	we previously reported that the aerolysin-like hemolysin of aeromonas sobria stimulates t84 cells to produce cyclic amp, which then emerges in the culture medium. in order to clarify the mechanism of action of the hemolysin, we examined the involvement of adenosine nucleotide. the results show that the hemolysin stimulates t84 cells to release atp, which is then converted to adenosine by ectonucleotidase. the adenosine generated might stimulate the p1 adenosine receptors of t84 cells to produce ...	2003	12595476
detection and characterization of the hemolysin genes in aeromonas hydrophila and aeromonas sobria by multiplex pcr.	a multiplex pcr assay was designed to amplify the aeromonas hydrophila and a. veronii bv. sobria hemolysin and aerolysin genes. the assay was evaluated by using 121 clinical isolates and 7 reference strains of aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex pcr. the five genotypes were characterized as type 1 for those carrying the ahh1 gene only (36% of isolates), type 2 for those carrying the asa1 gene only (8.5% of isolates), type 3 for ...	2003	12624028
bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates.	30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ...	2003	12644237
liver abscess and aeromonas bacteremia with septic pulmonary embolism.	a 68-year-old healthy man was admitted because of liver abscess and aeromonas bacteremia accompanied by pulmonary embolism. aeromonas sobria was isolated from blood. coagulation parameters were normal and a lower limb venogram showed no evidence of thrombosis. septic embolism originating from the liver abscess was considered as the possible cause of pulmonary embolism. the patient was successfully treated with antibiotics.	2003	14606725
distribution of virulence genes in aeromonas spp. isolated from sardinian waters and from patients with diarrhoea.	to characterize 46 isolates of different aeromonas spp. strains (26 aeromonas hydrophila, 13 aeromonas sobria and 7 aeromonas salmonicida) isolated from coastal water and clinical sources in sardinia, italy.	2002	11849349
novel plasmid-encoded class c beta-lactamase (mox-2) in klebsiella pneumoniae from greece.	klebsiella pneumoniae kol, a clinical strain resistant to various beta-lactams, was isolated from the stools of a patient from greece. this strain harbored a new pi 9.1 plasmid-mediated ampc beta-lactamase with unusually high levels of hydrolytic activity for cefoxitin and cefotetan that we named mox-2. sequencing of bla(mox-2) revealed 93.2, 92.9, 92.7, and 73.1% identities with the deduced amino acid sequences of cmy-8, mox-1, cmy-1, and the ampc beta-lactamase of aeromonas sobria, respectivel ...	2002	12069985
physicochemical and biological properties of an extracellular serine protease of aeromonas sobria.	previously, we cloned a protease gene of aeromonas sobria, determined its nucleotide sequence and established a method of purifying its product. in this study, we examined the properties of the purified protease. the protease was temperature-labile and had an optimal ph of 7.5. metallo-protease inhibitors and a cysteine protease inhibitor did not block the proteolytic activity of the enzyme. the treatment with reagents to modify sulfhydryl group did not reduce the activity. but, serine protease ...	2002	12153115
aeromonas culicicola sp. nov., from the midgut of culex quinquefasciatus.	the taxonomic position was examined of three isolates, mtcc 3249t, sh and slh, from the midgut of female culex quinquefasciatus and aedes aegyptii mosquitoes. numbers of cells of these isolates increased 2000-fold after a blood meal of the mosquitoes. 16s rrna gene sequence analysis of the novel strains showed that they were highly homologous to strains of aeromonas. dna-dna hybridization studies showed that dna of strain mtcc 3249t was 96 and 88% similar to that of strains sh and slh, respectiv ...	2002	12361279
the protein encoded at the 3' end of the serine protease gene of aeromonas sobria functions as a chaperone in the production of the protease.	for the successful production of aeromonas sobria serine protease (asp), open reading frame 2 (orf2) protein, encoded at the 3' end of the protease operon, is required. in this study, we examined the action of orf2 protein. the results showed that the protein associated with asp in the periplasm and helped asp to form an active structure.	2002	12446656
incidence of toxic aeromonas isolated from food and human infection.	one hundred and ninety four aeromonas isolates (99 from food and 95 from clinical sources) were analyzed as to the species involved and the toxins produced. of the clinical isolates of aeromonas, 29.4% were enterotoxigenic, 43.1% were hemolytic and 89% were cytotoxigenic. among the food isolates, 18.2% were enterotoxigenic, 17.1% were hemolytic and 72.7% were cytotoxigenic. aeromonas sobria and aeromonas veronii produced more enterotoxin and cytotoxin than the other isolates, whereas a. veronii ...	2002	11934569
[clinical and biological aspects of chronic brucellosis].	the present study refers to 53 cases of chronic brucellosis admitted to the clinic or followed up in an out-patient unit for ten years (1962-1971). there was a total of 946 days in hospital and 1688 days of occupational disability, about 40% of the patients necessitating repeated hospitalization because of the relapsing course of the disease. this group of cases presented the classical dominant features of an occupational anthropozoonoses (90.5%). elements of visceral and tissular pathology, obv ...	2002	128800
[pneumatization of the paranasal sinuses. causes and symptoms of hypopneumatization].		2002	127302
[experience in combination of the educational and scientific work of students].		2002	127158
induced transfer of higher plant chloroplasts into fungal protoplasts.	chloroplasts isolated from spinach leaves have been transferred in large numbers into protoplasts of neurospora crassa with the help of polyethylene glycol. the chloroplasts show high photosynthetic activity--at least until the time of uptake--and the protoplasts continue to show active cytoplasmic streaming after chloroplast uptake.	2002	127380
[postoperative esophageal stenosis (role of the laryngologist)].		2002	153373
post-menopausal oestrogens protect against fractures of hip and distal radius. a case-control study.	to assess the efficacy of oestrogens in preventing the common major limb fractures of postmenopausal women, retrospective case-control research was used for a new purpose--to explore beneficial rather than adverse effect of treatment. postmenopausal women admitted with fractures of the hip or distal radius and matched women admitted to the orthopaedic service for other reasons were surveyed. the "risk" of the non-oestrogen users, was estimated by inverting the customary odds ratio to calculate p ...	2001	90802
family life cycle resource center in the elementary school.		2001	98495
[immunohistochemical study of the differentiation of the cephalic lobe of the adenohypophysis in chick embryos].	it was shown by means of indirect immunofluorescence that acth appeared in the cephalic lobe of the chick embryo pituitary beginning from the 8th day of the development. a new tissue-specific antigen (a3) is revealed, which is localized in the cephalic lobe of adenohypophysis and becomes manifest at the 7th day of embryogenesis. quantitative analysis of acth and a3 localization in the cells of 11-day chick embryo adenohypophyses allows a conclusion that a3 is localized in corticotropic cells.	2001	88969
prostacyclin overproduction in bartter's syndrome.	urinary excretion of 6-keto-prostaglandin f1alpha and thromboxane b2, the major metabolites of prostacyclin and of thromboxane a2, respectively, was measured by specific radioimmunoassays in five female patients with bartter's syndrome and in five normal female controls. the patients with bartter's syndrome excreted about four times as much 6-keto-pgf1alpha as the controls; their excretion of thromboxane b2 was no different from that of the controls. these data suggest that overproduction of pro ...	2001	90861
parasitic and bacterial contamination in collards using effluent from treated domestic wastewater in chiang mai, thailand.	thailand often has inadequate water supply for agriculture during the dry season. the reuse of treated wastewater treatment plants could solve this problem. treatment of domestic wastewater of chiang mai municipality by the aerated lagoon system (al) releases more than 25,000 m3 of treated water everyday. the reuse of wastewater in agriculture is an efficient use of water, especially in tropical countries or in drought zones. the objective of this study is to demonstrate the possibility of using ...	2001	12041598
three cases of bacteraemia caused by aeromonas veronii biovar sobria.	septicaemia caused by aeromonas species is a life-threatening condition, arising primarily in immunocompromised patients, which has rarely been reported in scandinavia. herein we describe 3 cases of aeromonas sobria bacteraemia from denmark. all the patients were male and all 3 cases occurred during the summer. two patients had acute leukaemia and hiv infection, respectively, while the third patient had colorectal cancer diagnosed several years later. the clinical presentation in all patients wa ...	2001	11669236
isolation and identification of motile aeromonas species from chicken.	in this study a total of 140 broiler carcasses and carcass parts purchased at different supermarkets in ankara including 50 whole carcass, 30 wing, 30 leg and 30 breast samples were analysed for the presence of motile aeromonas species. according to analysis findings, motile aeromonas spp. were isolated from 116 (82.9%) of total 140 samples. the distribution of the isolates were 94%, 86.6%, 80%, 63.3% in broiler carcass, wing, leg and breast samples, respectively. aeromonas hydrophila was isolat ...	2001	11765602
aeromonas sobria sepsis in a neutropenic patient.		2001	11325663
characterization of aeromonas and vibrio species isolated from a drinking water reservoir.	to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species.	2001	11412322
[maturation pathway of hemolysin of aeromonas sobria and the mechanism of action of the hemolysin].	aeromonas sobria has been recognized as pathogens associated with acute gastroenteritis in both adults and children. the major virulence factor has been proposed to be hemolysin which possesses both hemolytic and enterotoxic activities. mature (bio-active) hemolysin secreted out of cells binds to the target cells of the host and injure the cells. however, hemolysin remained in bacteria can not express such toxicity. it means that the maturation and secretion pathway of hemolysin is closely relat ...	2001	11494595
an outbreak of acute gastroenteritis due to aeromonas sobria in benghazi, libyan arab jamahiriya.	we report an outbreak of acute diarrhoea due to aeromonas sobria in benghazi which occurred during a 1-month period in 1997. of 69 patients admitted with acute gastroenteritis, 28 were positive for a. sobria based on the production of gas from glucose, the production of acetoin, hydrogen sulfide and lysine decarboxylase and on aesculin hydrolysis and fermentation of arabinose and salicin. the strains were sensitive to chloramphenicol, co-trimoxazole, tetracycline and gentamicin but resistant to ...	2000	11556043
antimicrobial resistance of mesophilic aeromonas spp. isolated from two european rivers.	the activity of 19 antibiotics and four antiseptics and/or disinfectants was studied against 138 non-redundant strains of aeromonas spp. (104 aeromonas caviae, 22 aeromonas sobria and 12 aeromonas hydrophila) isolated from two european rivers. antibiotic resistance frequencies were: nalidixic acid, 59%; tetracycline, 14%; fosfomycin, 8%; tobramycin and cotrimoxazole, 7%; cefotaxime, 4%; chloramphenicol, 2%; gentamicin, 1%. most of the nalidixic acid-resistant strains were susceptible to fluoroqu ...	2000	10933657
inhibition of aeromonas caviae and a. sobria by sodium choloride, citric acid, ascorbic acid, potassium sorbate and extracts of thymus vulgaris.	the respective and combined effects of sodium chloride, ascorbic acid, citric acid, potassium sorbate, and thymus vulgaris extract on the growth of aeromonas caviae and aeromonas sobria were investigated. sodium chloride (3%) significantly reduced the growth and 4% nacl inhibited growth of the tested strains. ascorbic acid (0. 1%), potassium sorbate (0.05%), and citric acid (0.03%) slightly inhibited growth. t. vulgaris extract (0.3%) greatly reduced the growth. various combinations of these com ...	2000	10957708
[cytoplasmic protein vaccine against bacterial hemorrhagic septicemia (aeromonosis) of fish].	water-soluble proteins from aeromonas sobria, a causative agent of bacterial hemorrhagic septicemia of fishes, were separated into six fractions by gel chromatography on sephadex g-100. injections of fraction ii (67 kda) provided the highest protection of carps against the disease. injections of proteins contained in fraction ii caused stronger effects on certain biochemical parameters in the fish liver (fatty acids of phospholipids and cathepsin b and d activities) in comparison to infections o ...	2000	11042886
effect of sodium chloride and citric acid on growth and toxin production by a. caviae and a. sobria at moderate and low temperatures.	the effect of sodium chloride and citric acid on hemolysin and caseinase production by aeromonas caviae and aeromonas sobria at 32 degrees c and 5 degrees c was investigated. at 32 degrees c, although both strains were tolerant to 3% nacl in tsb, the production of caseinase was decreased in the presence of 1-3% nacl, and the production of hemolysin was abolished by 2-3% nacl. citric acid (0.03%) was less effective than nacl in reducing hemolysin and caseinase production by both strains at 32 deg ...	2000	11061632
production of serine protease of aeromonas sobria is controlled by the protein encoded by the gene lying adjacent to the 3' end of the protease gene.	we cloned a protease gene of aeromonas sobria and determined its nucleotide sequence. the protease is composed of 624 amino acid residues and its calculated molecular weight is 66,737.7. the amino acid sequence showed the characteristic features of a bacterial serine protease. we expressed the protease gene in vibrio parahaemolyticus from which the synthesized protease is secreted into the culture medium as the mature form, and purified the mature protease by successive column chromatographies. ...	2000	11092244
[microbiological monitoring of causative agents of sapronoses in the water of the bogatinskoe water reservoir].	the results of the microbiological monitoring of potential causative agents of sapronoses in the water of the bogatinskoye reservoir revealed that in the summer period of 1998 the mass accumulation of virulent aeromonas sobria (up to 25% of the total number of heterotrophic bacteria) took place. the autumn period was characterized by a decrease in the number of a. sobria and the detection of bacteria of the genus vibrio (up to 22%) with v. mimicus and v. metschnikovii identified among them in th ...	2000	11220958
carboxy terminal region of haemolysin of aeromonas sobria triggers dimerization.	haemolysin of aeromonas sobria is released into the culture supernatant in the form of prohaemolysin. removal of a 42 amino acid peptide at the carboxy-terminal end converts prohaemolysin into mature haemolysin. as the role of the peptide removed from the mature haemolysin has not been studied, we mutated the haemolysin genes to delete several amino acid residues from the carboxy terminus, expressed the mutant genes in a. sobria and analysed the haemolysins produced. deletion of more than three ...	2000	10623561
acute renal failure in an infant associated with cytotoxic aeromonas sobria isolated from patient's stool and from aquarium water as suspected source of infection.		2000	10681210
two-week postnatal examination of infants.		2000	87641
fetal antigens and prenatal diagnosis.		2000	89698
quantitation of 3-ethyl-5-hydroxy-4,5-dimethyl-delta 3-pyrrolin-2-one in the urine of patients with acute intermittent porphyria.		2000	110600
effect of dietary composition on fasting-induced changes in serum thyroid hormones and thyrotropin.		2000	97492
con-a-activated t cells secrete factors with polyclonal b-cell-activating properties.	concanavalin a induced polyclonal antibody synthesis in normal spleen cells in vitro. optimal responses were obtained by con a concentrations lower than those optimal for induction of dna synthesis. t cells, but not macrophages, were necessary for the effect. spleen cells from nude mice were not activated, whereas cells from the lps non-responder stain c3h/hej were activated to polyclonal antibody synthesis by con a. supernatants from con a activated spleen cells could by themselves induce polyc ...	2000	88758
the search for indices.		2000	91795
dithranol or photochemotherapy for psoriasis?		2000	86005
[evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy].		1999	88669
prenatal methylmercury poisoning. clinical observations over five years.	thirty-two infants prenatally exposed to methylmercury and their mothers were examined over a five-year period after the iraqi methylmercury epidemic. severity of poisoning in mothers was related to the peak mercury concentration in their hair and in the infants to the maximum concentration in maternal hair during pregnancy. in nine cases of cerebral palsy, methylmercury exposure occurred only during the last trimester. all infants except three (two were orphaned soon after birth and one was bot ...	1999	84530
[unit for bleaching acrylamide gels].		1999	91719
diminished cellular immunity due to impaired nutrition in oesophageal carcinoma.	the nutritional status of 15 negro patients suffering from unresectable carcinoma of the midthoracic oesophagus was evaluated before and after palliative pulsion intubation. all were shown to be in negative nitrogen balance and to have a compromised non-specific cellular immune response. correction of protein-calorie malnutrition and the achievement of positive nitrogen balance were associated with an increase in absolute lymphocyte and t lymphocyte numbers and a significant increase in lymphocy ...	1999	96903
increase in leg blood-flow by normovolaemic haemodilution in intermittent claudication.	normovolaemic haemodilution down to a packed-cell volume of 35% was done in 10 patients with stable intermittent claudication. for a mean reducation in the packed-cell volume of 21.5% there was a 170% increase in peak calf blood-flow and a 111% increase in haemoglobin delivery. 7 of the 10 patients reported an increased walking distance. normovolaemic haemodilution may be of use in the management of some patients with peripheral vascular disease.	1999	89279
thyrotoxicosis during pregnancy.	the diagnosis of thyrotoxicosis during pregnancy is difficult on clinical grounds only. the determination of free thyroid hormones or free thyroid hormone indices is important and is possibly supplemented by the trh stimulation test. thyrotoxicosis during pregnancy should always be treated actively. the authors recommend subtotal thyroidectomy if there are indications for surgical therapy (nodular goitre or large diffuse goitre). otherwise treatment with thyrostatic agents is used. after operati ...	1999	84547
haemodilution and blood-flow.		1999	88643
captopril in a hyponatraemic hypertensive: need for caution in initiating therapy.		1999	85145
combined infection of norwalk-like virus and verotoxin-producing bacteria associated with a gastroenteritis outbreak.	detection of multiple pathogens, particularly a combination of viruses and bacteria, is infrequently documented in outbreaks of gastroenteritis. this paper reports the presence of norwalk-like virus (nlv) and enterohaemorrhagic verotoxin-producing escherichia coli in one individual, and nlv and verotoxin-producing aeromonas sobria in another individual, both part of a large gastroenteritis outbreak. the causes of gastroenteritis in such outbreaks may be more complex than previously thought.	1999	10892495
secretion of hemolysin of aeromonas sobria as protoxin and contribution of the propeptide region removed from the protoxin to the proteolytic stability of the toxin.	the sequence at the amino terminus region of the hemolysin ofaeromonas sobria is homologous with that of aerolysin of a. hydrophila. however, there is no homology between the two toxins in the sequence at the carboxy terminal region. it has been shown that aerolysin is secreted into culture supernatant as a protoxin. this proaerolysin is activated by the proteolytic removal of a carboxy terminal peptide. however, the role of the carboxy terminal region, which is removed in the activation process ...	1999	10100744
unusual case of aeromonas sobria cellulitis associated with the use of leeches.		1999	10192721
[bacteriological studies of traveller's diarrhoea (6). analysis of enteropathogenic bacteria at kansai airport quarantine station from september 4th, 1994 through december 1996].	during the period of investigation from sept. 4, 1994 to dec, 1996, a total of 11,446,534 overseas travellers were quarantined at kansai airport quarantine station, and 22,187 voluntarily reported of episodes suffering from diarrhoea. bacteriological examination of the stools a total of 9,299 individuals' was performed, and the following results were obtained. 1) various enteropathogenic bacteria were isolated from 33.3% of the stools examined. bacterial species isolated were as follows: plesiom ...	1999	10213987
[rapidly fatal prognosis caused by aeromonas sobria septic shock in a patient with myelodysplastic syndrome].		1999	10222675
important aspects of the colonization of central venous catheter.	this study comprises five different kind of venous central catheters, 103 in total, made of polyurethane tecoflex, polyurethane vialon, ptfe and pvc, and the influence of their raw material on the microbial colonization. patients age and sex, besides their clinical conditions, were taken into account, and neither considered as a sample vicious, nor associated with colonization. when the tips of the catheters were asseptically inoculated in tryptic soy broth and tioglicolate, colonization was det ...	1999	10326311
enumeration and confirmation of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria isolated from raw milk and other milk products in northern greece.	a total of 138 raw cow's and 57 raw ewe's milk samples; 80 pasteurized cow's milk samples; 39 anthotyros cheese, 36 manouri cheese, and 23 feta cheese samples; and 15 rice pudding samples were examined for the presence and any countable population of aeromonas species. twenty-two (15.9%) of the 138 cow's milk samples analyzed were contaminated with a. hydrophila. in 13 of these samples, populations of 3.0x10(2) to 5.0x10(3) cfu/ml were counted in starch ampicillin agar (saa). eighteen cow's milk ...	1999	10340665
hemolysin of aeromonas sobria stimulates production of cyclic amp by cultured cells.	hemolysin of aeromonas sobria possesses both cytotoxic activity against mammalian cells and enterotoxic activity. histopathological examination revealed that hemolysin causes diarrhea without damaging the intestinal epithelial cells. and the fluid accumulated in the mouse intestinal loop by the action of the hemolysin is watery. these observations indicated that the enterotoxic activity of hemolysin is not dependent on its cytotoxic activity. to clarify the mechanism of the enterotoxic action of ...	1999	10418132
aeromonas sobria infection with severe soft tissue damage and segmental necrotizing gastroenteritis in a patient with alcoholic liver cirrhosis.	a 49-year-old man, who had a 3-year history of liver dysfunction but had not been treated, was admitted to the hospital with a sudden onset of fever and generalized muscle pain. he subsequently developed generalized purpura with scattered hemorrhagic bullae of the skin and massive bloody stools. aeromonas sobria was proven by culture of both blood and bullous fluid. in spite of the extensive treatment with antibiotics and other medications in the intensive care unit (icu), the patient went into ...	1999	10469397
identification and characterization of the aeromonas sobria hemolysin glycoprotein receptor on intestine 407 cells.	aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. by immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kda protein (p66) was identified as a receptor for a. sobria hemolysin on intestine 407 cells. treatment of p66 with n-glycosidase f reduced the apparent sized of p66 to 60 kda on sds-polyacrylamide gels. p66, released from intestine 407 cells following incubation with phosphatidylinositol-specific ph ...	1999	10502462
occurrence of haemolytic & cytotoxic aeromonas species in domestic water supplies in chennai.	a study on the occurrence of aeromonas species in the domestic water supplies in chennai showed that as much as 37.9 per cent of the water samples analyzed from various sources harbored aeromonas spp. majority of the isolates belonged to aeromonas sobria (13.7%), a. caviae (11.6%) and a. hydrophila (9.5%). among the 37 metropolitan water samples analyzed, 11 samples yielded aeromonas spp. inclusive of three isolates of a. hydrophila, four of a. sobria and two isolates each of a. caviae and a. ja ...	1999	10573655
virulence factors in aeromonas spp and their association with gastrointestinal disease.	culture filtrates of eight aeromonas strains isolated from the feces of 487 subjects (292 diarrhoeic patients and 195 asymptomatic subjects) were tested for toxin production in cho and mccoy cells, and adhesion and invasive ability in caco-2 cells. among these isolates, three aeromonas sobria and one aeromonas caviae strains possessed virulence-associated properties. toxin production was the most common of the three virulence properties. two a. caviae were associated, in the absence of other dia ...	1998	9497926
purification and characterization of enterotoxin produced by aeromonas sobria.	we purified the toxin of aeromonas sobria capable of inducing a positive response in the mouse intestinal loop assay. the purified toxin showed a positive response not only in the loop assay but also in a hemolytic assay. subsequently, we cloned the toxin gene and demonstrated that the product of this gene possessed both hemolytic and enterotoxic activities. these results showed that the enterotoxin of a. sobria possesses hemolytic activity. nucleotide sequence determination of the toxin gene an ...	1998	9858466
[meningitis by aeromonas sobria following cranio-encephalic traumatism].		1998	9919002
molecular cloning, nucleotide sequence, and expression in escherichia coli of a hemolytic toxin (aerolysin) gene from aeromonas trota.	aeromonas trota ak2, which was derived from atcc 49659 and produces the extracellular pore-forming hemolytic toxin aerolysin, was mutagenized with the transposon mini-tn5km1 to generate a hemolysin-deficient mutant, designated strain ak253. southern blotting data indicated that an 8.7-kb noti fragment of the genomic dna of strain ak253 contained the kanamycin resistance gene of mini-tn5km1. the 8.7-kb noti dna fragment was cloned into the vector pgem5zf(-) by selecting for kanamycin resistance, ...	1998	9647817
occurrence, diversity and pathogenicity of mesophilic aeromonas in estuarine waters of the italian coast of the adriatic sea.	a total of 208 strains of aeromonas were isolated by monthly sampling from two estuaries (one provided with, and the other devoid of a waste-water treatment system) on the italian coast of the adriatic sea between september 1994 and august 1995. biotyping at the species level allowed the identification of 96 strains (46%) as aer. caviae, 46 (22%) as aer. sobria, 33 (16%) as aer. hydrophila and 25 (12%) as aer. veronii. eight strains (4%) were regarded as unnamed aeromonads. aeromonas caviae was ...	1998	9750281
the acute phase response in the sudan plated lizard, gerrhosaurus major.	the sudan plated lizard (gerrhosaurus major), previously reported to be an afebrile species, was utilized in a series of experiments to test for various aspects of the acute phase response. treatment of individuals with the antibiotic baytril resulted in a slight (0.5 degree c) but significant reduction in mean selected body temperature (msbt), while treatment with saline did not lower msbt. nonantibiotic treatment individuals had depressed plasma iron levels (86.6 +/- 22.4 micrograms fe 100 ml- ...	1998	9773512
a case of acute cholecystitis due to aeromonas sobria and hafnia alvei from northern europe.		1998	11864254
lipase activity and gene cloning of acinetobacter calcoaceticus lp009.	the production of lipase from acinetobacter calcoaceticus lp009, a bacterium isolated from raw milk, was found to be best induced by tween-80 at 1.0% concentration. it was efficiently secreted, and only a minute amount of activity was detected at the cell surface and intracellularly. a. calcoaceticus lp009 lipase exhibited maximum activity at ph 7.0 and 50 degrees c, and was relatively stable upon storage at ph 5.0 to 7.0 and at 4, 30, or 37 degrees c. the enzyme was found to be inactivated by e ...	1998	12501281
[unusual fetomaternal blood group antagonism].		1998	105299
[general anesthesia and long term psychotropic treatments].	anesthesists often ask for withdrawal of psychotropic treatment a few days before surgery. usually they do not differentiate among the various classes of psychotropic drugs. some drugs, including mao inhibitors and reserpine-like agents, can induce or precipitate accidents; however, this does not occur with other types of antidepressants, neuroleptics and minor tranquilizers. the authors review the psychiatric risks related to such withdrawals; these risks are to be considered. they report that ...	1998	105865
cerebrospinal fluid-, blood-, and ascitis-catecholamines in hepatic cirrhosis.	the consideration of the extensive innervation of liver parenchyma, together with data relating to the degradation of catecholamines in the liver and the importance of hepatic vascular process in hepatic cirrhosis, prompted us to investigate the behaviour of catecholamines in this condition. the following parameters were measured in cerebrospinal fluid (csf), blood and ascitis fluid: total catecholamines, adrenaline and noradrenaline. during hepatic cirrhosis, total catecholamines level in blood ...	1998	106413
[pancreatic carcinoma on endoscopic retrograde pancreatico-cholangiogram (author's transl)].	the pancreaticographic appearances of carcinoma of the pancreas have been divided into two types: 1. canalicular carcinoma arising from the duct system. if arising from the main pancreatic duct, it occludes this, or causes displacement or deformity of its branches in the immediate neighbourhood. if arising from branches, it causes cystic ectasia of the minor ducts; these appear fragmented and deformed while there is stenosis and displacement of the main duct. simultaneous origin from the main an ...	1998	124684
health expenditure 1978/79.		1998	104698
[x-ray study of the stomach. what is it used for and what is it worth?].		1998	105438
["masked" supra-renal tumour (author's transl)].	for the diagnosis of supra-renal tumours or tumours of supra-renal rests, it is necessary to employ all available radiological methods as well as the clinical data. two cases are described in which a supra-renal tumour had been "masked" and found at operation. in the first case a cyst in the tail of the pancreas had been considered, in the second, a tumour of the renal hilum or ureter had been diagnosed. it is concluded that in the differential diagnosis of a cyst of the tail of the pancreas, su ...	1998	124685
acute rhabdomyolysis and myonecrosis complicating aeromonas bacteremia in neutropenic patients with hematologic malignancies: report of two cases.	infections by aeromonas spp. are a rare cause of systemic infection in normal and immunocompromised hosts. we report the cases of two patients with acute non-lymphoblastic leukemia who developed septic shock by aeromonas species with unusual soft-tissue complications. one patient who was undergoing consolidation chemotherapy developed septic shock by aeromonas hydrophila with rhabdomyolysis and subsequent soft-tissue destruction consistent with myonecrosis. she recovered with combination antibio ...	1997	9499669