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incidence and virulence characteristics of aeromonas spp. in fish.this study was conducted to evaluate the presence of aeromonas spp. in raw and ready-to-eat (rte) fish commonly consumed in assiut city, egypt, and to determine virulence factors due to they play a key role in their pathogenicity.201728246446
antibacterial effect of gallic acid against aeromonas hydrophila and aeromonas sobria through damaging membrane integrity.in the study, we investigated the antibacterial activity and mechanism of gallic acid against aeromonas hydrophila and aeromonas sobria. gallic acid showed strong antimicrobial activity against the two bacteria. furthermore, the antibacterial mechanism of gallic acid (0, 3, 6, 12 mm) was performed by membrane integrity assay and scanning electron microscopy (sem) assay. the results showed that gallic acid notably increased the released material absorption value at 260, 280 nm and electric conduc ...201727774889
probiotic potential of autochthonous bacteria isolated from the gastrointestinal tract of four freshwater teleosts.in this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (channa striatus), striped dwarf catfish (mystus vittatus), orangefin labeo (labeo calbasu) and mrigal carp (cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, aeromonas hydrophila, aeromonas salmonicida, aeromonas sobria and pseudomonas fluorescens and were non-hemolytic. the isol ...201727557836
ethmoiditis with subperiosteal and retro-ocular abscesses due to aeromonas sobria in a 16-year-old boy exposed to the ardèche river. 201728343158
post-translationally modified frog skin-derived antimicrobial peptides are effective against aeromonas sobria.antimicrobial peptides (brevinin1 hyba1 and brevinin1 hyba2) identified from the skin secretion of an endemic frog species of western ghats were studied against fish pathogens. post-translational modifications such as c-terminal amidation and cyclization of the peptides were enhanced on the activity against aeromonas sobria. based on the minimum inhibitory concentration (3 μm), cyclic amidated brevinin hyba2 was identified as the most promising antimicrobial agent against a. sobria and can be de ...201728153544
draft genome sequence of aeromonas sobria strain 08005, isolated from sick rana catesbeiana.aeromonas sobria is a gram-negative, rod-shaped, and ubiquitous bacterium. we present here the draft genome sequence of a. sobria strain 08005, isolated from an infected bullfrog. it is composed of 66 contigs totaling 4,678,951 bp, contains 4,252 coding dna sequences (cdss), four rrnas, and 88 trna sequences, and shows the presence of various putative virulence-related genes.201728082483
a truncated sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in oryzias melastigma.antimicrobial peptides (amps) represent an efficient part of innate immunity and are found in a variety of life. among them histone 2a (h2a), as a promising class of amps, attracts great attention, but the in vivo mechanism of h2a derived amp is still less known. based on the acquisition of sphistin, a synthetic 38-amino acid h2a derived peptide from scylla paramamosain, as reported in our previous study, was truncated into three short fragments (sph12-38, sph20-38 and sph30-38) and further inve ...201728600196
determination of spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing and culture-dependent method.this study was conducted to determine the initial and spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing (ngs) and a culture-dependent method. the quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (tvb-n) values. after 1 d of storage, the psychrotrophic bacteria were only 5.97 log cfu/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log cfu/g). the psychrotrophic bacteria counts exceed ...201728399332
in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus.fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ...201728819794
hematopoietic neoplasms accompanied by severe enterocolitis due to aeromonas species.aeromonas species are known to be a cause of diarrhea and acute enterocolitis. however, only a few cases have been reported and the pathophysiology of aeromonas infection has not as yet been clarified. we experienced 2 cases developing severe enterocolitis during the course of hematological malignancies, specifically multiple myeloma and diffuse large b-cell lymphoma. both patients presented with watery diarrhea that persisted for more than a week, followed by bloody diarrhea. total colon endosc ...201728484157
curcumin liposomes interfere with quorum sensing system of aeromonas sobria and in silico analysis.aeromonas sobria is opportunistic pathogen frequently found in environment and food. interfering with its quorum sensing (qs) system could be a promising way to alleviate its virulence. in this study, curcumin liposomes were prepared and their characteristics like particle size, zeta potential, pdi (polymey disperse index), encapsulation efficiency and loading capacity were measured. the quorum sensing inhibitory effect of curcumin liposomes under sub-mic (minimum inhibitory concentration) on si ...201728819178
efficacy of spirulina platensis diet supplements on disease resistance and immune-related gene expression in cyprinus carpio l. exposed to herbicide atrazine.the present study evaluated the immunotoxicological effects of the herbicide atrazine (atz) at sub-lethal concentrations and the potential ameliorative influence of spirulina platensis (sp) over a sub-chronic exposure period on cyprinus carpio l., also known as common carp. common carp was sampled after a 40-days exposure to atz (428 μg/l) and sp (1%), individually or in combination to assess the non-specific immune response, changes in mrna expression of immune-related genes [lysozyme (lyz), im ...201728579523
aeromonas sobria, an outbreak of natural infection in cultured silver catfish rhamdia quelen (quoy & gaimard, 1824) in argentina. 201728548705
oxa-427, a new plasmid-borne carbapenem-hydrolysing class d β-lactamase in enterobacteriaceae.to describe a novel plasmid-borne class d carbapenemase (chdl) named oxa-427 identified in several enterobacteriaceae clinical isolates from nine patients in one belgian hospital.201728859446
strong genomic and phenotypic heterogeneity in the aeromonas sobria species complex.aeromonas sobria is a mesophilic motile aeromonad currently depicted as an opportunistic pathogen, despite increasing evidence of mutualistic interactions in salmonid fish. however, the determinants of its host-microbe associations, either mutualistic or pathogenic, remain less understood than for other aeromonad species. on one side, there is an over-representation of pathogenic interactions in the a. sobria literature, of which only three articles to date report mutualistic interactions; on th ...201729276504
involvement of the arg566 residue of aeromonas sobria serine protease in substrate specificity.aeromonas sobria serine protease (asp) is an extracellular serine protease secreted by the organism. here, we identified the amino acid residue of asp that contributes to substrate specificity by using both synthetic peptides and biological protein components. the results showed that the arginine residue at position 566 (arg-566) of asp, which is located in the extra occluding region of asp close to an entrance of the catalytic cavity, is involved in the substrate specificity. a substitutional p ...201729023605
secondary bacterial infections and antibiotic resistance among tungiasis patients in western, kenya.tungiasis or jigger infestation is a parasitic disease caused by the female sand flea tunga penetrans. secondary infection of the lesions caused by this flea is common in endemic communities. this study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions and their susceptibility profiles to commonly prescribed antibiotics. participants were recruited with the help of community health workers. swabs were taken from lesions which showed signs of second ...201728886013
aeromonas sobria serine protease (asp): a subtilisin family endopeptidase with multiple virulence activities.aeromonas sobria serine protease (asp) is secreted from aeromonas sobria, a pathogen causing gastroenteritis and sepsis. asp resembles saccharomyces cerevisiae kex2, a member of the subtilisin family, and preferentially cleaves peptide bonds at the c-terminal side of paired basic amino acid residues; also accepting unpaired arginine at the p1 site. unlike kex2, however, asp lacks an intramolecular chaperone n-terminal propeptide, instead utilizes the external chaperone orf2 for proper folding, t ...201728432839
detection and characterization of potentially pathogenic aeromonas sobria isolated from fish hypophthalmichthys molitrix (cypriniformes: cyprinidae).the current study focuses on the detection and characterization of potentially pathogenic aeromonas sobria from fish silver carp (hypophthalmichthys molitrix). assessment of clinical, microbiological, pathological and biochemical characteristics of a. sobria were taken into account in order to understand the epidemiology, frequency and occurrence of this infection. clinically the infected fish (h. molitrix) was observed for various types of symptoms. a total of 33 colonies of a. sobria strain we ...201626518124
protective potency of clove oil and its transcriptional down-regulation of aeromonas sobria virulence genes in african catfish (clarias gariepinus l.).disease episodes of fish caused by aeromonas species are moved to the top list of limiting problems worldwide. the present study was planned to verify the in vitro antibacterial activities as well as the in vivo potential values of clove oil and ciprofloxacin against aeromonas sobria in african catfish (clarias gariepinus). the in vitro phenotypic virulence activities and the successful amplification of aerolysin and hemolysin genes in the precisely identified a. sobria strain were predictive fo ...201627609474
stress and humoral innate immune response of gilthead seabream sparus aurata cultured in sea cages.innate and acquired immune responses of gilthead seabream sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in alghero bay, italy. every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. innate humoral immunity, such as compl ...201627485027
design of an immersion vaccine against aeromonad septicemia in perch (perca fluviatilis l.).the production of an immersion vaccine and the vaccination procedure to immunize fry of perch (perca fluviatilis l.) against pathogenic aeromonas sobria that harbor a type iii secretion system is described. the vaccine, based on chemically inactivated a. sobria, enables rapid vaccination of a large number of fish by immersion of fry in an aqueous vaccine suspension during 5 min, giving them high protection during fattening under open water conditions in a freshwater lake for at least 4 months.201627076300
effects of dietary acidifiers on growth, hematology, immune response and disease resistance of nile tilapia, oreochromis niloticus.organic acids or their salts can be used as feed additives in aquaculture. this study was conducted to evaluate the use of a mixture of formic acid, propionic acid and calcium propionate compared with oxytetracycline (otc). a total of 720 apparently healthy oreochromis niloticus fingerlings with an average body weight of 28.8 ± 0.14 g (mean ± se) were randomly divided into four equal groups (g1, g2, g3and g4). g1 was fed a basal diet with no additive as the control group, while g2 and g3 were fe ...201626860238
indolo[3,2-b]quinoline derivatives suppressed the hemolytic activity of beta-pore forming toxins, aerolysin-like hemolysin produced by aeromonas sobria and alpha-hemolysin produced by staphylococcus aureus.in an attempt to discover inhibitory compounds against pore-forming toxins, some of the major toxins produced by bacteria, we herein examined the effects of four kinds of indolo[3,2-b]quinoline derivatives on hemolysis induced by the aerolysin-like hemolysin (alh) of aeromonas sobria and also by the alpha-hemolysin of staphylococcus aureus. the results showed that hemolysis induced by alh was significantly reduced by every derivative, while that induced by alpha-hemolysis was significantly reduc ...201626725434
antibiogram characterization and putative virulence genes in aeromonas species isolated from pig fecal samples.aeromonas species are broadly distributed in nature and agricultural environments and have been isolated from feces, bedding, and drinking water of healthy pigs. we assessed the incidence, virulence properties, and antimicrobial resistance profile of aeromonas spp., isolated from pig feces. antibiogram was done using the disc diffusion methods, and polymerase chain reaction was used for the detection of putative virulence genes. identification of isolates revealed three phenotypic species with p ...201626971520
microangiopathic hemolytic anemia. 20164913945
involvement of acylated homoserine lactones (ahls) of aeromonas sobria in spoilage of refrigerated turbot (scophthalmus maximus l.).one quorum sensing strain was isolated from spoiled turbot. the species was determined by 16s rrna gene analysis and classical tests, named aeromonas sobria as7. quorum-sensing (qs) signals (n-acyl homoserine lactones (ahls)) were detected by report strains and their structures were further determined by gc-ms. the activity changes of ahls on strain growth stage as well as the influence of different culture conditions on secretion activity of ahls were studied by the punch method. the result ind ...201627420072
degradation products and the role of coagulation in "persistent" glomerulonephritis. 20164931741
resonance energy transfer in pepsin conjugates. 20164897805
[closed fracture of the hyoid bone body with the formation of the false joint]. 20164767991
modern practice in immunization. 10. tetanus immunization. 20164884415
[application of isotopes to heart-pulmonary circulation dynamics]. 20164871482
preventing and detecting leakage in epidural catheters. 20164856942
growth in height of children with scoliosis. 20154463687
sudden death from neurological causes in india. 20155025982
specific sensitivity of neural crest cells in mice embryos to alkylating agents and hydrocortisone acetate. 20154479284
[double contrast radiography of the stomach using a radiographic image intensifier]. 20154409271
temporal and contextual cues as discriminative attributes in retardates' recognition memory. 20154704210
[diagnosis of edema]. 20154488702
induction of -globin synthesis in the -thalassaemia of ferrara. 20154505416
cystic fibrosis and coeliac disease. report of two cases. 20154733638
the estimation of serum creatine phosphokinase activity using a reaction-rate analyser. 20154581093
molecular cloning and functional characterization of mannose receptor in zebra fish (danio rerio) during infection with aeromonas sobria.mannose receptor (mr) is a member of pattern-recognition receptors (prrs), which plays a significant role in immunity responses. much work on mr has been done in mammals and birds while little in fish. in this report, a mr gene (designated as zfmr) was cloned from zebra fish (danio rerio), which is an attractive model for the studies of animal diseases. the full-length cdna of zfmr contains 6248 bp encoding a putative protein of 1428 amino acids. the predicted amino acid sequences showed that zf ...201525988382
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland.we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ...201525380369
[aeromonas sobria infection in patients with acute leukemia:clinical features and outcome].to investigate the clinical features and outcome of patients with acute leukemia and aeromonas sobria infection.201526725395
a report of peritonitis from aeromonas sobria in a peritoneal dialysis (pd) patient with necrotizing fasciitis.a 70-years of age, male patient with underlying type 2 diabetes mellitus, hypertension, dyslipidemia and ischemic heart disease had undergone continuous ambulatory peritoneal dialysis (capd)for 3 years without any episodes of peritonitis. he was diagnosed with necrotizing fasciitis and later developed peritonitis after receiving a laceration from an aquatic injury suffered during the flood disaster of 2011. the blood culture, necrotic tissue and the clear dialysate collected upon admission had s ...201527276849
aeromonas sobria infection in farmed mud loach (misgurnus mizolepis) in korea, a bacteriological survey.a disease outbreak occurred in june 2012 among mud loach cultured on pond farms in jangseong-gun, jeollanam-do, korea. mortality rates reached up to 1.2% in the farm per day. typical clinical signs were bleeding ulcer at the middle portion of head and haemorrhagic erosion of the operculum. based on biochemical characteristics, the causative bacterium isolated from diseased fish was identified as aeromonas sobria. the isolate expressed two haemolytic genes, aerolysin (sob) and haemolysin (asa1) g ...201527175175
structure of the o-specific polysaccharide from the lipopolysaccharide of aeromonas sobria strain pt312.the o-specific polysaccharide (ops) obtained by mild-acid degradation of the lipopolysaccharide from aeromonas sobria strain pt312 was studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d 1h,1h cosy, tocsy, noesy, 1h-detected 1h,13c hsqc, and hmbc experiments. the sequence of the sugar residues was determined using 1h,1h noesy and 1h,13c hmbc experiments. it was found that the ops was built up of disaccharide repeating units composed of glcpnac and non-s ...201525037826
microbiological and parasitological investigation on chelonians reared in italian facilities.the rapid rise in the number of pet chelonians and their illegal trade can modify the ecology, involving exotic pets, humans, and microbiological agents. therefore, different epidemiological situations and the related risk to introduce and spread infectious diseases, especially zoonotic agents, have to be considered. the aim of this study was to investigate the microbiological and parasitological situation in 2 chelonian facilities (a private breeding of tortoises and a shelter for turtles) coll ...201526344661
structural basis for action of the external chaperone for a propeptide-deficient serine protease from aeromonas sobria.subtilisin-like proteases are broadly expressed in organisms ranging from bacteria to mammals. during maturation of these enzymes, n-terminal propeptides function as intramolecular chaperones, assisting the folding of their catalytic domains. however, we have identified an exceptional case, the serine protease from aeromonas sobria (asp), that lacks a propeptide. instead, orf2, a protein encoded just downstream of asp, appears essential for proper asp folding. the mechanism by which orf2 functio ...201525784551
[fatal pedestrian-accidents in street traffic (author's transl)]. 20154774662
aeromonas sobria necrotizing fasciitis and sepsis in an immunocompromised patient: a case report and review of the literature.aeromonas veronii biovar sobria is a rare cause of bacteremia, with several studies indicating that this isolate may be of particular clinical significance since it is enterotoxin producing. a wide spectrum of infections has been associated with aeromonas species in developing countries that include gastroenteritis, wound infections, septicemia and lung infections. this infection, caused by aeromonas species, is usually more severe in immunocompromised than immunocompetent individuals. we here d ...201425245365
antibacterial efficacy of recombinant siganus oramin l-amino acid oxidase expressed in pichia pastoris.siganus oraminl-amino acid oxidase is a novel natural protein (named sr-laao) isolated from serum of the rabbitfish (s. oramin), which showed antibacterial activity against both gram-positive and gram-negative bacteria and had a lethal effect on the parasites cryptocaryon irritans, trypanosoma brucei brucei and ichthyophthirius multifiliis. in order to test whether recombinant sr-laao (rsr-laao) produced by the eukaryotic expression system also has antimicrobial activity, the yeast pichia pastor ...201425238719
the manx shearwater (puffinus puffinus) as a candidate sentinel of atlantic ocean health.seabirds have been historically used to monitor environmental contamination. the aim of the present study was to test the suitability of a species belonging to the procellariiformes group, the manx shearwater, puffinus puffinus, as a sentinel of environmental health, by determining contaminant levels (trace metals and organochlorine compounds) from carcass tissues and by isolating vibrio spp. and aeromonas spp. from live specimens. to this end, 35 puffinus puffinus carcasses wrecked on the north ...201425191536
cdna cloning and expression characterization of serum transferrin gene from oriental weatherfish misgurnus anguillicaudatus.in this study, the cdna coding serum transferrin (stf) of misgurnus anguillicaudatus (mastf) was cloned. mastf cdna is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. the deduced mastf protein consists of a signal peptide, two lobes (n and c-lobes) and signature motifs of transferrin (tf) family. the results of tissue distribution indicated that mastf mrna was predominantly expressed in the liver. the results indicate that the mastf expression increased significan ...201424673686
the in-vitro antibacterial effects of organic salts, chemical disinfectants and antibiotics against pathogens of black disease in fairy shrimp of thailand.the antibacterial effects of organic salts, chemical disinfectants and antibiotics were evaluated on cultures of aeromonas hydrophila c03, aeromonas sobria c26, a. sobria c29, aeromonas caviae c24 and acinetobacter sp. sh-94b, the pathogens that cause black disease found in fairy shrimps (streptocephalus sirindhornae sanoamuang et al. (2000) and branchinella thailandensis sanoamuang, saengphan & murugan) of thailand. the minimal inhibitory concentrations (mics) of organic salts (sodium chloride ...201424131294
detection of wilson's disease in sibs of a patient with wilson's disease. 20143943210
morphological novelty in the limb skeleton accompanies miniaturization in salamanders.salamanders of the genus thorius (plethodontidae) are among the smallest tetrapods. hypotheses of limb skeletal evolution in these vertebrates were evaluated on the basis of estimates of natural variation, comparisons of skeletal homology, and analysis of molecular phylogeny. nine carpal arrangements occur in thorius, more than in all twelve related genera of typically larger salamanders; six of these arrangements are unique. they represent a trend toward a decrease in the number of separate car ...20144023715
[evolution of phonation from early infancy to puberty: electro-glottographic study]. 20144251834
[treatment with kushen chinpi decoction in 50 cases of acute bacillary dysentery in children]. 20144200749
factors influencing prognosis in carcinoma of the stomach.prognostic factors were prospectively sought in a consecutive group of 223 patients with carcinoma of the stomach observed for a minimum of seven years. age, race and mode of presentation did not influence survival time. statistically improved seven year survival rates were observed in females (p less than 0.01) and patients with several features: symptoms lasting more than six months (p less than 0.01); absence of an abdominal mass (p less than 0.01); t1 versus t3 stage of disease (p less than ...20143961656
[5-hyroxytryptamine in the central nervous system of the gold- fish (carassius auratus)]. 20144177370
[indications and technic of proximal selective vagotomy of the gastric acid secretory zone]. 20144283143
[electrophoretic study of cerebrospinal fluid proteins in gliomas of the cerebral hemispheres]. 20144299992
[spontaneous hematomas of the rectus abdominis]. 20144264255
propoxyphene. two fatalities due to overdose. 20144684062
[status of motor neurons and the monosynaptic reflex arc before a simple voluntary movement]. 20144043622
[colovesical fistulas]. 20143948747
british medical association attacks cigarette smoking. 20143978493
consumptive coagulopathy associated with aortic aneurysm. 20133605176
a new species of the early tertiary rodent thisbemys. 20133716069
the politician-doctor nexus. 20133564142
the liver and metabolic bone disease. 20133837818
[successful delivery in a female with thrombotic thrombocytopenic purpura]. 20133820614
effectiveness of thymostimulin treatment in hepatitis b surface antigen-positive chronic active liver disease. results of a randomized clinical trial.the results of this controlled trial with thymostimulin (tp-1 serono) on hepatitis b surface antigen (hbsag)-positive chronic active liver disease (cald), involving 34 consecutive patients chosen by predefined criteria and randomly allocated to control and treatment groups, show that this thymic hormone may significantly improve the clinical, biochemical, serological and immunological parameters of this disease, and ameliorate the quality of the patients' life. the treatment schedule was adjuste ...20133907642
effectiveness of thymostimulin treatment in hepatitis b surface antigen-positive chronic active liver disease. results of a randomized clinical trial.the results of this controlled trial with thymostimulin (tp-1 serono) on hepatitis b surface antigen (hbsag)-positive chronic active liver disease (cald), involving 34 consecutive patients chosen by predefined criteria and randomly allocated to control and treatment groups, show that this thymic hormone may significantly improve the clinical, biochemical, serological and immunological parameters of this disease, and ameliorate the quality of the patients' life. the treatment schedule was adjuste ...20133907642
[how to evaluate diseases of the mitral valve]. 20133845722
deer slaughter. 20133705401
[the origins of huttonian thought: hutton, student and physician]. 20134629584
[value of systematic toxicological analysis in the management of drug addicts].this study was carried out between march and december 1985, on 50 drug addicts, including 45 heroin users, hospitalized for detoxication. we compared the clinical informations obtained at the time of admission with the toxicological informations obtained from blood and urinary samples. if the drugs said to have been used over the last 48 hours are always found, in 52% of these cases some other drugs are found too. the reasons of this difference between clinical and toxicological informations are ...20133665817
[role of calcium channel blockers in diseases of the circulatory system]. 20133613400
[medical data and patients' opinion on the hospital regimen]. 20133847237
molecular cloning and functional characterization of a short peptidoglycan recognition protein (hcpgrps1) from the freshwater mussel, hyriopsis cumingi.peptidoglycan recognition proteins (pgrps), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. in the present study, a short-form pgrp, designated as hcpgrps1 was identified from freshwater mussel hyriopsis cumingi. the deduced amino acid sequence of hcpgrps1 is composed of 235 residues which contains a conserved pgrp domain at the c-terminus. sequence analysis showed that hcpgrps1 shared high identitie ...201323933513
molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (megalobrama amblycephala).β-defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. in this study, the part-length cdna sequences of two β-defensin genes (maβd-1, maβd-2) in blunt snout bream (megalobrama amblycephala) were identified. homology analysis showed that the cdna sequences of maβd-1 and maβd-2 had high similarities to those in common carp and zebrafish. real-time quantitative pcr results exhibited that expre ...201323876385
adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against saprolegnia parasitica .biological control of saprolegniosis with bacteria might be an alternative to the use of chemical compounds. among criteria for the selection of such bacteria are their absence of pathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. the pathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity against saprolegnia parasitica was studied. fifteen of the isolates, identified as aeromonas sobria, pantoea agglomerans, pseudomon ...201323670078
skin and soft-tissue infections caused by aeromonas sobria. 201323584005
characterization of the bacterial microbiota of biomphalaria glabrata (say, 1818) (mollusca: gastropoda) from brazil.roughly 200 000 000 people in 74 countries infected with schistosomes all share the fact that they came in contact freshwater harbouring infected snails. the aim of the study is to characterize the microbiota of wild and laboratory-reared snails of biomphalaria glabrata from pernambuco, brazil. the microbiota of these molluscs was identified biochemically by the vitek 2 automated microbiological system. antimicrobial susceptibility testing was carried out by the disc diffusion method with ß-lact ...201323488866
detection of cyprinid herpesvirus 2 in association with an aeromonas sobria infection of carassius carassius (l.), in italy.sixteen specimens of female crucian carp, carassius carassius (l.), during the breeding season, were investigated for post-mortem and full diagnostic examination during a mortality outbreak in a tributary stream of the arno river in tuscany in 2011. necropsy highlighted the presence of a swollen anus and widespread haemorrhages in the body, fins, gills and eyes. haemorrhages in internal organs and spleen granulomas were also observed. bacteria isolated from the brain, kidney and spleen of affect ...201323488630
molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from simensis crocodile (crocodylus siamensis).hepcidin, a cysteine-rich cationic antibacterial peptide, plays an important role in human defense against pathogen infection. however, its role in reptile immune response and whether it is involved in antibacterial immune have not yet been proven. in order to study the antibacterial activity of crocodylus siamensis hepcidin (cshepc), a common reptile which lives in topic region of southeast asia, a cdna sequence of cshepc was cloned, which included an open reading frame (orf) of 300 bp encoding ...201322967859
production and properties of lipase of aeromonas sobria.aeromonas have been isolated from a wide variety of aquatic environments. however the number of aeromonas in sea water is extremely small compared to that in fresh water. in in vitro culture, aeromonas can grow in mediums containing nacl at a concentration of 3.0%, this concentration corresponding to that of sea water. it is unclear why the number of aeromonas is low in sea water. exoproteins of bacteria are thought to be important for bacterial growth and survival in the environment. previously ...201222376235
mortality of therapeutic fish garra rufa caused by aeromonas sobria.to investigate a case of mass mortality of garra rufa (g. rufa) from a fish hatchery farm in slovakia.201223569873
fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients.infection with aeromonas species has been reported to occur in neutropenic patients. necrotizing fasciitis caused by aeromonas species is uncommon but potentially life-threatening. we herein describe three cases of fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients. these cases shared many clinical characteristics, including shock, coagulopathy, multiple organ failure and rapidly deteriorating and eventually fatal clinical courses. in all cases, aeromonas sobria w ...201223207126
inhibition of aeromonas sobria serine protease (asp) by α2-macroglobulin.asp is a serine protease secreted by aeromonas sobria. asp cleaves various plasma proteins, which is associated with onset of sepsis complications, such as shock and blood coagulation disorder. to investigate a host defense mechanism against this virulence factor, we examined the plasma for asp inhibitor(s). human plasma inhibited asp activity for azocasein, which was almost completely abolished by treating plasma with methylamine, which inactivates α2-macroglobulin (α2-mg). the asp-inhibitor co ...201223089609
the tail nick augments aeromonas sobria serine protease (asp) activity in plasma through retarding inhibition by α2-macroglobulin.asp is a serine protease secreted by aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. the pathogen also secretes nasp that has a nick in the carboxy-terminal region. compared with single-chain asp (sasp), nasp had near-equivalent activity for small peptide substrates but was less proteolytic. surprisingly, nasp cleaved proteins more in plasma and was inhibited by human α(2)-macroglobulin more slowly than sasp. retarded inhibiti ...201222971340
first description of cole-type plasmid in aeromonas spp. carrying quinolone resistance (qnrs2) gene.isolation and full sequence analysis of cole-type plasmid, which carries the qnrs2 gene.201222862417
recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda.the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ...201222564558
molecular detection of the aeromonas virulence aerolysin gene in retail meats from different animal sources in egypt.meat commonly contain the same aeromonas spp. which occur in human diarrhoeal and non-diarrhoeal faecal samples. motile aeromonas were isolated from 5.6% of total 302 samples. the distribution of the isolates were 5.9 and 5.2% in fresh and frozen samples, respectively. of the 302 samples taken of the four animal meat species investigated, the genus aeromonas were isolated in 12.3% of the fresh samples collected from buffalo meat, in 6.5% of the samples collected from sheep meat and 14.0% from th ...201222806009
bacterial sepsis following prostatic biopsy.despite the recent innovations, complications of prostate biopsy can occur. the aim of this study was a prospective monitoring of major septic complications occurring after transrectal prostate biopsy, to describe their causing agents, to report the clinical course of these patients, and to give guidelines based on our personal experience.201222371127
[how many people undergo treatment with a psychiatrist?]. 20123648811
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