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simple and direct detection of aeromonas hydrophila infection in the goldfish, carassius auratus (l.), by dot blotting using specific monoclonal antibodies.a combination of eight isolates of aeromonas hydrophila was used to produce monoclonal antibodies (mabs). ten different groups of mabs specific to aeromonas were selected. the first five groups of mabs demonstrated high specificity and bound to only one or two isolates of a. hydrophila. the sixth and the seventh groups of mabs were a. hydrophila specific. they recognized seven of eight a. hydrophila isolates (ah1, 2, 3, 4, 5, 6, 8); however, the mab in the seventh group also showed cross-reactiv ...201021091724
cellular components of probiotics control yersinia ruckeri infection in rainbow trout, oncorhynchus mykiss (walbaum).subcellular components of the probiotics aeromonas sobria gc2 and bacillus subtilis jb-1, when administered to rainbow trout, oncorhynchus mykiss, conferred protection against a new biogroup of yersinia ruckeri. thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (cwps), outer membrane proteins (omps), lipopolysaccharides (lps), whole cell proteins (wcps) and live cells followed by challenge on day 8 with y. ruckeri led to 80-100% survival compared with 10% ...201019912460
association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of anodonta cygnea (linnaeus, 1758).bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. a complete understanding of the balance between anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of a. cygnea collected from mira lagoon in northern portugal. the results showed vibrio metschnikovii and aeromonas sobria as the dominant groups. th ...201020349058
aeromonas fluvialis sp. nov., isolated from a spanish river.a gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(t), was isolated from a water sample collected from the muga river, girona, north-east spain. preliminary analysis of the 16s rrna gene sequence showed that this strain belonged to the genus aeromonas, the nearest species being aeromonas veronii (99.5 % similarity, with seven different nucleotides). a polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrb, rpod, reca, dnaj and g ...201019648345
maturation pathway of metalloprotease produced by aeromonas sobria.previously, we cloned the metalloprotease gene of aeromonas sobria (amp) and determined its nucleotide sequence (genbank accession number dq784565). the protease is composed of 591 amino acid residues. in this study, we purified the mature metalloprotease from the culture supernatant of a. sobria and determined the amino terminal sequence and molecular size of amp. in addition, we examined the production of amp diachronically and found that amp emerges outside of the cell as an intermediate comp ...201021118297
enhancement of protective immunity in european eel (anguilla anguilla) against aeromonas hydrophila and aeromonas sobria by a recombinant aeromonas outer membrane protein.to develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (omp) antigen from different bacterial pathogens. in this study, an omp fragment of 747 bp (named as omp-g), which was highly conserved in seven aeromonas omp sequences from the ncbi database, was amplified by pcr from one aeromonas sobria strain (b10) and two aeromonas hydrophila strains (b27 and b33) with the designed specific prim ...201021148192
post-translational processing of anglerfish islet somatostatin precursors.two distinct somatostatin precursors are synthesized in anglerfish (af) islets. in addition to a precursor which has somatostatin 14 (ss-14) as a c-terminal cleavage product, a precursor which contains at its c-terminus [tyr7, gly10] ss-14 as a potential cleavage product is also synthesized. however, even though an arg-lys pair is located immediately n-terminal to ala1 of the c-terminal tetradecapeptide, [tyr7, gly10]ss-14 was not found in significant amounts in extracts of af islets. instead, a ...20102863927
raising the standards. the right tools for the job. 20103405819
studies on 2(1h)-quinolinone derivatives as neuroleptic agents i. synthesis and biological activities of (4-phenyl-1-piperazinyl)-propoxy-2(1h)-quinolinone derivatives. 20102907733
malignant melanoma. 20103768643
aeromonas sobria bacteriemia in an acute lymphoblastic leukemia case in remission. 201127263949
bioaffinity sensors. 20113131631
pattern-evoked cortical potentials and nuclear magnetic resonance imaging in total and partial agenesis of the corpus callosum.we have examined two cases of agenesis of the corpus callosum without any associated abnormalities. they were accidentally detected in x-ray-computed tomography. total agenesis of the corpus callosum could be differentiated by nuclear magnetic resonance imaging from partial agenesis. they had moderately depressed stereopsis. visually evoked cortical potentials to pattern stimuli showed abnormal findings in binocular viewing, and vecp equi-potential maps were similar to those of normal controls.20114053962
darkroom diseases and how to combat them. 20113112384
radiographic assessment of knee alignment after total knee arthroplasty.standard anteroposterior standing radiographs (14 x 17-inch cassettes) and full-limb radiographs (51-inch) from a randomized group of 50 patients undergoing total knee arthroplasty between january 1985 and june 1986 were analyzed. the mean tibiofemoral angle on short films was 5.8 degrees valgus, compared with 7.2 degrees valgus on full-limb films. this 1.4 degrees discrepancy had a standard deviation of 2.2 degrees, emphasizing the measurement variability in any individual case. the distance fr ...20113361322
[new aspects of vitamin k prophylaxis in newborn infants and infants].discussion about vitamin k prophylaxis has again become a matter of major concern since cases of life threatening bleeding due to late onset vitamin k deficiency have been observed in germany. this review tries to summarize present knowledge about the clinical presentation, pathogenesis and the potential prevention of vitamin k deficiency caused bleeding in the neonatal period and infancy.20113785234
[lumbalgia among adolescents]. 20113158780
inhibition of biosynthesis of metalloprotease of aeromonas sobria by sodium chloride in the medium.the present authors have previously shown that the serine protease activity of aeromonas sobria is markedly decreased when a. sobria is cultured in medium containing 3.0% sodium chloride (nacl, concentration almost equivalent to sea water salinity), and that this occurs because, although the synthesis of asp is not disturbed by the salt in the medium, the maturation pathway of serine protease of a. sobria (asp) does not proceed successfully in such a medium. in this study, the effect of salt in ...201121175775
the influence of environmental bacteria in freshwater stingray wound-healing.invasion by bacteria can influence the course of healing of wounds acquired in aquatic environment. in this study, the bacteria present in potamotrygonmotoro stingray mucus and in the alto paraná river water were identified, and their ability to induce tissue injury and resist antibiotics was determined. biochemical identification analysis showed that 97% of all bacterial isolates were gram negative, aeromonas spp., enterobacter cloacae and citrobacter freundii being the species most prevalent. ...201121635911
three goose-type lysozymes in the gastropod oncomelania hupensis: cdna sequences and lytic activity of recombinant proteins.three goose-type (g-type) lysozymes, designated as ohlysg1, ohlysg2 and ohlysg3 were identified from expressed sequence tags (ests) of a gastropod oncomelania hupensis, the intermediate host of schistosoma japonicum. the full cdna sequences of ohlysg1, ohlysg2 and ohlysg3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the n-terminus, respectively. the three g-type lysozymes shared conserv ...201121756934
[preparation and identification of ompw monoclonal antibodies].to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw.201121906472
Structural and Functional Analysis of the Serine Protease from Aeromonas sobria.Aeromonas species are Gram-negative facultative anaerobic bacteria found ubiquitously in a variety of aquatic environments and most commonly implicated as causative agents of gastroenteritis. Sepsis is a fatal complication of Aeromonas infectious diseases, particularly in immune-compromised patients. Two species, Aeromonas hydrophila and Aeromonas sobria, are associated with human disease. Feasible virulence factors of Aeromonas include fimbrial and afimbrial adhesion molecules, hemolysins, ente ...201122129876
[fatal aeromonas sobria infection in liver cirrhosis].aeromonas sobria infection is known to be very serious in immunocompromised hosts. we report a case of a. sobria infection fatal in a subject with decompensated liver cirrhosis. a 63-year-old man with liver cirrhosis admitted for fever and vomiting developed a necrotizing soft-tissue infection in the right lower leg. despite a decompression incision in the affected limb and intensive care, he died of sepsis one day after surgery. a. sobria was detected afterward in a blood culture.201122117380
aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet.two freshwater isolates (wb4.1-19(t) and wb4.4-101), sharing 99.9 % 16s rrna gene sequence similarity, were highly related to aeromonas sobria (99.7 % similarity; 6 bp differences). a phylogenetic tree derived from a multi-locus phylogenetic analysis (mlpa) of the concatenated sequences of five housekeeping genes (gyrb, rpod, reca, dnaj and gyra; 3684 bp) revealed that both strains clustered as an independent phylogenetic line next to members of aeromonas molluscorum and aeromonas bivalvium. the ...201120207806
quorum sensing in aeromonas species isolated from patients in malaysia.bacterial quorum sensing signal molecules called n-acylhomoserine lactone (ahl) controls the expression of virulence determinants in many gram-negative bacteria. we determined ahl production in 22 aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). all isolates produced the two principal ahls, n-butanoylhomoserine lactone (c4-hsl) and n-hexanoyl homoserine lactone (c6-hsl). ten isolates also produced additional ahls. this re ...201120544198
identification and pathogenicity of aeromonas sobria on tail-rot disease in juvenile tilapia oreochromis niloticus.thirty-six strains, numbered from py01 to py36, were isolated from six moribund oreochromis niloticus. the biochemical characteristics of all strains conformed to the species description of aeromonas sobria on the basis of api 20e and biolog gn system. furthermore, gyrb sequence of strain py36 was sequenced and showed high similarity (99.8%) with a. sobria in genbank. antibiotic-resistance of strain py36 was assessed by the kirby-bauer disk diffusion method, and the results showed it was suscept ...201120853167
bcr-abl oncogene renders myeloid cell line factor independent: potential autocrine mechanism in chronic myeloid leukemia.in chronic myeloid leukemia (cml), a chromosome translocation has fused the bcr gene to the c-abl oncogene, such that a chimeric bcr-abl polypeptide can be made. to explore the biological properties of bcr-abl and compare them with those of the abelson virus (amulv) transforming gene (gag-v-abl), we have used either a synthetic bcr-v-abl gene that mimics the translocation product or, in some experiments, a bcr-c-abl cdna. a new retroviral vector was used to introduce the genes into the factor-de ...20113147434
[pit and fissure sealants. the effect of etching depends on the differences in tooth surface cleaning methods, and the method of cleaning pits and fissures]. 20123526496
infection caused by aeromonas sobria, complicated by lower extremity paraplegia and the cauda equine syndrome in a patient with well-controlled type 2 diabetes. 201223671455
use of computerized data listings and activity profiles of genetic and related effects in the review of 195 compounds.computer-generated listings of data from short-term tests for genetic and related effects (activity profile listings) were prepared for 195 compounds that included for each compound, the test system (identified by a three-letter code word), qualitative results and the lowest effective dose (led) or highest ineffective dose (hid) tested. a corresponding bar or line graph (activity profile) was also generated, in which test systems are displayed along the x-axis and the led or hid values along the ...20123367921
[february 1948 and the further development of czechoslovak pharmacy]. 20123282688
very early presentation of pompe's disease and its cross-sectional echocardiographic features.pompe's disease was diagnosed in a 23-day-old female infant with congestive cardiac failure and hypotonia. the cross-sectional echocardiographic features of this case are described. the possible implications of this extremely early presentation are discussed.20123308716
[protein studies in cystic and peritoneal fluids of ovarian tumors with special reference to cea].the levels of total protein, ig, afp, beta-hcg, cea, sc, sp1 and alpha 2-pag were measured in cystic and peritoneal effusions obtained from 70 females with benign and malignant ovarian tumors. this study was carried out to test out parameters those are useful for tumor monitoring of ovarian cancer. our results indicate that determining cea in peritoneal effusions could be one of useful methods in tumor monitoring of mucinous cystadenocarcinomas in cases of known histology and praetherapeutic est ...20123376613
wine, women, suspiciousness and advertising.women college students (n = 66) viewed videotapes of a soap opera episode and a rock music program, ostensibly to evaluate the appeal of such material to college women. different versions of the videotape included zero, three or nine wine commercials. refreshments, including white wine, were available to the subjects. suspiciousness about the experiment was evaluated through an open-ended questionnaire, responses to which were independently content-analyzed by two scorers. major findings were as ...20123560952
wine, women, suspiciousness and advertising.women college students (n = 66) viewed videotapes of a soap opera episode and a rock music program, ostensibly to evaluate the appeal of such material to college women. different versions of the videotape included zero, three or nine wine commercials. refreshments, including white wine, were available to the subjects. suspiciousness about the experiment was evaluated through an open-ended questionnaire, responses to which were independently content-analyzed by two scorers. major findings were as ...20123560952
[properties of lak cell activity and lak-generation in pbl isolated from patients with renal cell carcinoma]. 20123266646
a word about words. 20123459834
malignant hyperthermia in a renal transplant recipient. 20123318564
[how many people undergo treatment with a psychiatrist?]. 20123648811
[how many people undergo treatment with a psychiatrist?]. 20123648811
astigmatism after strabismus surgery. 20123347457
culturable gut microbiota diversity in zebrafish.the zebrafish (danio rerio) is an increasingly used laboratory animal model in basic biology and biomedicine, novel drug development, and toxicology. the wide use has increased the demand for optimized husbandry protocols to ensure animal health care and welfare. the knowledge about the correlation between culturable zebrafish intestinal microbiota and health in relation to environmental factors and management procedures is very limited. a semi-quantitative level of growth of individual types of ...201222428747
have you looked at your will lately? seven common mistakes in estate planning and how to avoid them. 20123373137
fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients.infection with aeromonas species has been reported to occur in neutropenic patients. necrotizing fasciitis caused by aeromonas species is uncommon but potentially life-threatening. we herein describe three cases of fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients. these cases shared many clinical characteristics, including shock, coagulopathy, multiple organ failure and rapidly deteriorating and eventually fatal clinical courses. in all cases, aeromonas sobria w ...201223207126
inhibition of aeromonas sobria serine protease (asp) by α2-macroglobulin.asp is a serine protease secreted by aeromonas sobria. asp cleaves various plasma proteins, which is associated with onset of sepsis complications, such as shock and blood coagulation disorder. to investigate a host defense mechanism against this virulence factor, we examined the plasma for asp inhibitor(s). human plasma inhibited asp activity for azocasein, which was almost completely abolished by treating plasma with methylamine, which inactivates α2-macroglobulin (α2-mg). the asp-inhibitor co ...201223089609
the tail nick augments aeromonas sobria serine protease (asp) activity in plasma through retarding inhibition by α2-macroglobulin.asp is a serine protease secreted by aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. the pathogen also secretes nasp that has a nick in the carboxy-terminal region. compared with single-chain asp (sasp), nasp had near-equivalent activity for small peptide substrates but was less proteolytic. surprisingly, nasp cleaved proteins more in plasma and was inhibited by human α(2)-macroglobulin more slowly than sasp. retarded inhibiti ...201222971340
molecular detection of the aeromonas virulence aerolysin gene in retail meats from different animal sources in egypt.meat commonly contain the same aeromonas spp. which occur in human diarrhoeal and non-diarrhoeal faecal samples. motile aeromonas were isolated from 5.6% of total 302 samples. the distribution of the isolates were 5.9 and 5.2% in fresh and frozen samples, respectively. of the 302 samples taken of the four animal meat species investigated, the genus aeromonas were isolated in 12.3% of the fresh samples collected from buffalo meat, in 6.5% of the samples collected from sheep meat and 14.0% from th ...201222806009
bacterial sepsis following prostatic biopsy.despite the recent innovations, complications of prostate biopsy can occur. the aim of this study was a prospective monitoring of major septic complications occurring after transrectal prostate biopsy, to describe their causing agents, to report the clinical course of these patients, and to give guidelines based on our personal experience.201222371127
production and properties of lipase of aeromonas sobria.aeromonas have been isolated from a wide variety of aquatic environments. however the number of aeromonas in sea water is extremely small compared to that in fresh water. in in vitro culture, aeromonas can grow in mediums containing nacl at a concentration of 3.0%, this concentration corresponding to that of sea water. it is unclear why the number of aeromonas is low in sea water. exoproteins of bacteria are thought to be important for bacterial growth and survival in the environment. previously ...201222376235
mortality of therapeutic fish garra rufa caused by aeromonas sobria.to investigate a case of mass mortality of garra rufa (g. rufa) from a fish hatchery farm in slovakia.201223569873
diathermy burns. 20123696207
first description of cole-type plasmid in aeromonas spp. carrying quinolone resistance (qnrs2) gene.isolation and full sequence analysis of cole-type plasmid, which carries the qnrs2 gene.201222862417
recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda.the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ...201222564558
cervical lymph node metastases with occult primary.patients with cervical metastases from an unknown primary tumour present both diagnostic and therapeutic problems. twenty patients with a metastatic lesion in the neck, in whom the primary tumour was not found despite extensive, diagnostic procedures, were treated in the department of otolaryngology, linköping university hospital, during a 14-year period (1971-1984). since 1975 tonsillectomy has been performed regularly on the affected side as a part of the diagnostic procedure. eight patients w ...20123815873
molecular cloning and functional characterization of a short peptidoglycan recognition protein (hcpgrps1) from the freshwater mussel, hyriopsis cumingi.peptidoglycan recognition proteins (pgrps), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. in the present study, a short-form pgrp, designated as hcpgrps1 was identified from freshwater mussel hyriopsis cumingi. the deduced amino acid sequence of hcpgrps1 is composed of 235 residues which contains a conserved pgrp domain at the c-terminus. sequence analysis showed that hcpgrps1 shared high identitie ...201323933513
molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from simensis crocodile (crocodylus siamensis).hepcidin, a cysteine-rich cationic antibacterial peptide, plays an important role in human defense against pathogen infection. however, its role in reptile immune response and whether it is involved in antibacterial immune have not yet been proven. in order to study the antibacterial activity of crocodylus siamensis hepcidin (cshepc), a common reptile which lives in topic region of southeast asia, a cdna sequence of cshepc was cloned, which included an open reading frame (orf) of 300 bp encoding ...201322967859
molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (megalobrama amblycephala).β-defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. in this study, the part-length cdna sequences of two β-defensin genes (maβd-1, maβd-2) in blunt snout bream (megalobrama amblycephala) were identified. homology analysis showed that the cdna sequences of maβd-1 and maβd-2 had high similarities to those in common carp and zebrafish. real-time quantitative pcr results exhibited that expre ...201323876385
adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against saprolegnia parasitica .biological control of saprolegniosis with bacteria might be an alternative to the use of chemical compounds. among criteria for the selection of such bacteria are their absence of pathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. the pathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity against saprolegnia parasitica was studied. fifteen of the isolates, identified as aeromonas sobria, pantoea agglomerans, pseudomon ...201323670078
skin and soft-tissue infections caused by aeromonas sobria. 201323584005
characterization of the bacterial microbiota of biomphalaria glabrata (say, 1818) (mollusca: gastropoda) from brazil.roughly 200 000 000 people in 74 countries infected with schistosomes all share the fact that they came in contact freshwater harbouring infected snails. the aim of the study is to characterize the microbiota of wild and laboratory-reared snails of biomphalaria glabrata from pernambuco, brazil. the microbiota of these molluscs was identified biochemically by the vitek 2 automated microbiological system. antimicrobial susceptibility testing was carried out by the disc diffusion method with ß-lact ...201323488866
detection of cyprinid herpesvirus 2 in association with an aeromonas sobria infection of carassius carassius (l.), in italy.sixteen specimens of female crucian carp, carassius carassius (l.), during the breeding season, were investigated for post-mortem and full diagnostic examination during a mortality outbreak in a tributary stream of the arno river in tuscany in 2011. necropsy highlighted the presence of a swollen anus and widespread haemorrhages in the body, fins, gills and eyes. haemorrhages in internal organs and spleen granulomas were also observed. bacteria isolated from the brain, kidney and spleen of affect ...201323488630
[medical data and patients' opinion on the hospital regimen]. 20133847237
[successful delivery in a female with thrombotic thrombocytopenic purpura]. 20133820614
effectiveness of thymostimulin treatment in hepatitis b surface antigen-positive chronic active liver disease. results of a randomized clinical trial.the results of this controlled trial with thymostimulin (tp-1 serono) on hepatitis b surface antigen (hbsag)-positive chronic active liver disease (cald), involving 34 consecutive patients chosen by predefined criteria and randomly allocated to control and treatment groups, show that this thymic hormone may significantly improve the clinical, biochemical, serological and immunological parameters of this disease, and ameliorate the quality of the patients' life. the treatment schedule was adjuste ...20133907642
effectiveness of thymostimulin treatment in hepatitis b surface antigen-positive chronic active liver disease. results of a randomized clinical trial.the results of this controlled trial with thymostimulin (tp-1 serono) on hepatitis b surface antigen (hbsag)-positive chronic active liver disease (cald), involving 34 consecutive patients chosen by predefined criteria and randomly allocated to control and treatment groups, show that this thymic hormone may significantly improve the clinical, biochemical, serological and immunological parameters of this disease, and ameliorate the quality of the patients' life. the treatment schedule was adjuste ...20133907642
[role of calcium channel blockers in diseases of the circulatory system]. 20133613400
[value of systematic toxicological analysis in the management of drug addicts].this study was carried out between march and december 1985, on 50 drug addicts, including 45 heroin users, hospitalized for detoxication. we compared the clinical informations obtained at the time of admission with the toxicological informations obtained from blood and urinary samples. if the drugs said to have been used over the last 48 hours are always found, in 52% of these cases some other drugs are found too. the reasons of this difference between clinical and toxicological informations are ...20133665817
[the origins of huttonian thought: hutton, student and physician]. 20134629584
[how to evaluate diseases of the mitral valve]. 20133845722
deer slaughter. 20133705401
consumptive coagulopathy associated with aortic aneurysm. 20133605176
a new species of the early tertiary rodent thisbemys. 20133716069
the politician-doctor nexus. 20133564142
the liver and metabolic bone disease. 20133837818
[indications and technic of proximal selective vagotomy of the gastric acid secretory zone]. 20144283143
[electrophoretic study of cerebrospinal fluid proteins in gliomas of the cerebral hemispheres]. 20144299992
[spontaneous hematomas of the rectus abdominis]. 20144264255
propoxyphene. two fatalities due to overdose. 20144684062
[status of motor neurons and the monosynaptic reflex arc before a simple voluntary movement]. 20144043622
[colovesical fistulas]. 20143948747
british medical association attacks cigarette smoking. 20143978493
[evolution of phonation from early infancy to puberty: electro-glottographic study]. 20144251834
detection of wilson's disease in sibs of a patient with wilson's disease. 20143943210
morphological novelty in the limb skeleton accompanies miniaturization in salamanders.salamanders of the genus thorius (plethodontidae) are among the smallest tetrapods. hypotheses of limb skeletal evolution in these vertebrates were evaluated on the basis of estimates of natural variation, comparisons of skeletal homology, and analysis of molecular phylogeny. nine carpal arrangements occur in thorius, more than in all twelve related genera of typically larger salamanders; six of these arrangements are unique. they represent a trend toward a decrease in the number of separate car ...20144023715
[treatment with kushen chinpi decoction in 50 cases of acute bacillary dysentery in children]. 20144200749
factors influencing prognosis in carcinoma of the stomach.prognostic factors were prospectively sought in a consecutive group of 223 patients with carcinoma of the stomach observed for a minimum of seven years. age, race and mode of presentation did not influence survival time. statistically improved seven year survival rates were observed in females (p less than 0.01) and patients with several features: symptoms lasting more than six months (p less than 0.01); absence of an abdominal mass (p less than 0.01); t1 versus t3 stage of disease (p less than ...20143961656
antibacterial efficacy of recombinant siganus oramin l-amino acid oxidase expressed in pichia pastoris.siganus oraminl-amino acid oxidase is a novel natural protein (named sr-laao) isolated from serum of the rabbitfish (s. oramin), which showed antibacterial activity against both gram-positive and gram-negative bacteria and had a lethal effect on the parasites cryptocaryon irritans, trypanosoma brucei brucei and ichthyophthirius multifiliis. in order to test whether recombinant sr-laao (rsr-laao) produced by the eukaryotic expression system also has antimicrobial activity, the yeast pichia pastor ...201425238719
the manx shearwater (puffinus puffinus) as a candidate sentinel of atlantic ocean health.seabirds have been historically used to monitor environmental contamination. the aim of the present study was to test the suitability of a species belonging to the procellariiformes group, the manx shearwater, puffinus puffinus, as a sentinel of environmental health, by determining contaminant levels (trace metals and organochlorine compounds) from carcass tissues and by isolating vibrio spp. and aeromonas spp. from live specimens. to this end, 35 puffinus puffinus carcasses wrecked on the north ...201425191536
aeromonas sobria necrotizing fasciitis and sepsis in an immunocompromised patient: a case report and review of the literature.aeromonas veronii biovar sobria is a rare cause of bacteremia, with several studies indicating that this isolate may be of particular clinical significance since it is enterotoxin producing. a wide spectrum of infections has been associated with aeromonas species in developing countries that include gastroenteritis, wound infections, septicemia and lung infections. this infection, caused by aeromonas species, is usually more severe in immunocompromised than immunocompetent individuals. we here d ...201425245365
cdna cloning and expression characterization of serum transferrin gene from oriental weatherfish misgurnus anguillicaudatus.in this study, the cdna coding serum transferrin (stf) of misgurnus anguillicaudatus (mastf) was cloned. mastf cdna is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. the deduced mastf protein consists of a signal peptide, two lobes (n and c-lobes) and signature motifs of transferrin (tf) family. the results of tissue distribution indicated that mastf mrna was predominantly expressed in the liver. the results indicate that the mastf expression increased significan ...201424673686
[5-hyroxytryptamine in the central nervous system of the gold- fish (carassius auratus)]. 20144177370
the in-vitro antibacterial effects of organic salts, chemical disinfectants and antibiotics against pathogens of black disease in fairy shrimp of thailand.the antibacterial effects of organic salts, chemical disinfectants and antibiotics were evaluated on cultures of aeromonas hydrophila c03, aeromonas sobria c26, a. sobria c29, aeromonas caviae c24 and acinetobacter sp. sh-94b, the pathogens that cause black disease found in fairy shrimps (streptocephalus sirindhornae sanoamuang et al. (2000) and branchinella thailandensis sanoamuang, saengphan & murugan) of thailand. the minimal inhibitory concentrations (mics) of organic salts (sodium chloride ...201424131294
aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland.we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ...201525380369
microbiological and parasitological investigation on chelonians reared in italian facilities.the rapid rise in the number of pet chelonians and their illegal trade can modify the ecology, involving exotic pets, humans, and microbiological agents. therefore, different epidemiological situations and the related risk to introduce and spread infectious diseases, especially zoonotic agents, have to be considered. the aim of this study was to investigate the microbiological and parasitological situation in 2 chelonian facilities (a private breeding of tortoises and a shelter for turtles) coll ...201526344661
structural basis for action of the external chaperone for a propeptide-deficient serine protease from aeromonas sobria.subtilisin-like proteases are broadly expressed in organisms ranging from bacteria to mammals. during maturation of these enzymes, n-terminal propeptides function as intramolecular chaperones, assisting the folding of their catalytic domains. however, we have identified an exceptional case, the serine protease from aeromonas sobria (asp), that lacks a propeptide. instead, orf2, a protein encoded just downstream of asp, appears essential for proper asp folding. the mechanism by which orf2 functio ...201525784551
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
a report of peritonitis from aeromonas sobria in a peritoneal dialysis (pd) patient with necrotizing fasciitis.a 70-years of age, male patient with underlying type 2 diabetes mellitus, hypertension, dyslipidemia and ischemic heart disease had undergone continuous ambulatory peritoneal dialysis (capd)for 3 years without any episodes of peritonitis. he was diagnosed with necrotizing fasciitis and later developed peritonitis after receiving a laceration from an aquatic injury suffered during the flood disaster of 2011. the blood culture, necrotic tissue and the clear dialysate collected upon admission had s ...201527276849
aeromonas sobria infection in farmed mud loach (misgurnus mizolepis) in korea, a bacteriological survey.a disease outbreak occurred in june 2012 among mud loach cultured on pond farms in jangseong-gun, jeollanam-do, korea. mortality rates reached up to 1.2% in the farm per day. typical clinical signs were bleeding ulcer at the middle portion of head and haemorrhagic erosion of the operculum. based on biochemical characteristics, the causative bacterium isolated from diseased fish was identified as aeromonas sobria. the isolate expressed two haemolytic genes, aerolysin (sob) and haemolysin (asa1) g ...201527175175
molecular cloning and functional characterization of mannose receptor in zebra fish (danio rerio) during infection with aeromonas sobria.mannose receptor (mr) is a member of pattern-recognition receptors (prrs), which plays a significant role in immunity responses. much work on mr has been done in mammals and birds while little in fish. in this report, a mr gene (designated as zfmr) was cloned from zebra fish (danio rerio), which is an attractive model for the studies of animal diseases. the full-length cdna of zfmr contains 6248 bp encoding a putative protein of 1428 amino acids. the predicted amino acid sequences showed that zf ...201525988382
[aeromonas sobria infection in patients with acute leukemia:clinical features and outcome].to investigate the clinical features and outcome of patients with acute leukemia and aeromonas sobria infection.201526725395
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