Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation and identification of motile aeromonas species from chicken. | in this study a total of 140 broiler carcasses and carcass parts purchased at different supermarkets in ankara including 50 whole carcass, 30 wing, 30 leg and 30 breast samples were analysed for the presence of motile aeromonas species. according to analysis findings, motile aeromonas spp. were isolated from 116 (82.9%) of total 140 samples. the distribution of the isolates were 94%, 86.6%, 80%, 63.3% in broiler carcass, wing, leg and breast samples, respectively. aeromonas hydrophila was isolat ... | 2001 | 11765602 |
| aeromonas sobria sepsis in a neutropenic patient. | 2001 | 11325663 | |
| characterization of aeromonas and vibrio species isolated from a drinking water reservoir. | to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species. | 2001 | 11412322 |
| [maturation pathway of hemolysin of aeromonas sobria and the mechanism of action of the hemolysin]. | aeromonas sobria has been recognized as pathogens associated with acute gastroenteritis in both adults and children. the major virulence factor has been proposed to be hemolysin which possesses both hemolytic and enterotoxic activities. mature (bio-active) hemolysin secreted out of cells binds to the target cells of the host and injure the cells. however, hemolysin remained in bacteria can not express such toxicity. it means that the maturation and secretion pathway of hemolysin is closely relat ... | 2001 | 11494595 |
| parasitic and bacterial contamination in collards using effluent from treated domestic wastewater in chiang mai, thailand. | thailand often has inadequate water supply for agriculture during the dry season. the reuse of treated wastewater treatment plants could solve this problem. treatment of domestic wastewater of chiang mai municipality by the aerated lagoon system (al) releases more than 25,000 m3 of treated water everyday. the reuse of wastewater in agriculture is an efficient use of water, especially in tropical countries or in drought zones. the objective of this study is to demonstrate the possibility of using ... | 2001 | 12041598 |
| novel plasmid-encoded class c beta-lactamase (mox-2) in klebsiella pneumoniae from greece. | klebsiella pneumoniae kol, a clinical strain resistant to various beta-lactams, was isolated from the stools of a patient from greece. this strain harbored a new pi 9.1 plasmid-mediated ampc beta-lactamase with unusually high levels of hydrolytic activity for cefoxitin and cefotetan that we named mox-2. sequencing of bla(mox-2) revealed 93.2, 92.9, 92.7, and 73.1% identities with the deduced amino acid sequences of cmy-8, mox-1, cmy-1, and the ampc beta-lactamase of aeromonas sobria, respectivel ... | 2002 | 12069985 |
| the protein encoded at the 3' end of the serine protease gene of aeromonas sobria functions as a chaperone in the production of the protease. | for the successful production of aeromonas sobria serine protease (asp), open reading frame 2 (orf2) protein, encoded at the 3' end of the protease operon, is required. in this study, we examined the action of orf2 protein. the results showed that the protein associated with asp in the periplasm and helped asp to form an active structure. | 2002 | 12446656 |
| incidence of toxic aeromonas isolated from food and human infection. | one hundred and ninety four aeromonas isolates (99 from food and 95 from clinical sources) were analyzed as to the species involved and the toxins produced. of the clinical isolates of aeromonas, 29.4% were enterotoxigenic, 43.1% were hemolytic and 89% were cytotoxigenic. among the food isolates, 18.2% were enterotoxigenic, 17.1% were hemolytic and 72.7% were cytotoxigenic. aeromonas sobria and aeromonas veronii produced more enterotoxin and cytotoxin than the other isolates, whereas a. veronii ... | 2002 | 11934569 |
| distribution of virulence genes in aeromonas spp. isolated from sardinian waters and from patients with diarrhoea. | to characterize 46 isolates of different aeromonas spp. strains (26 aeromonas hydrophila, 13 aeromonas sobria and 7 aeromonas salmonicida) isolated from coastal water and clinical sources in sardinia, italy. | 2002 | 11849349 |
| physicochemical and biological properties of an extracellular serine protease of aeromonas sobria. | previously, we cloned a protease gene of aeromonas sobria, determined its nucleotide sequence and established a method of purifying its product. in this study, we examined the properties of the purified protease. the protease was temperature-labile and had an optimal ph of 7.5. metallo-protease inhibitors and a cysteine protease inhibitor did not block the proteolytic activity of the enzyme. the treatment with reagents to modify sulfhydryl group did not reduce the activity. but, serine protease ... | 2002 | 12153115 |
| aeromonas culicicola sp. nov., from the midgut of culex quinquefasciatus. | the taxonomic position was examined of three isolates, mtcc 3249t, sh and slh, from the midgut of female culex quinquefasciatus and aedes aegyptii mosquitoes. numbers of cells of these isolates increased 2000-fold after a blood meal of the mosquitoes. 16s rrna gene sequence analysis of the novel strains showed that they were highly homologous to strains of aeromonas. dna-dna hybridization studies showed that dna of strain mtcc 3249t was 96 and 88% similar to that of strains sh and slh, respectiv ... | 2002 | 12361279 |
| [clinical and biological aspects of chronic brucellosis]. | the present study refers to 53 cases of chronic brucellosis admitted to the clinic or followed up in an out-patient unit for ten years (1962-1971). there was a total of 946 days in hospital and 1688 days of occupational disability, about 40% of the patients necessitating repeated hospitalization because of the relapsing course of the disease. this group of cases presented the classical dominant features of an occupational anthropozoonoses (90.5%). elements of visceral and tissular pathology, obv ... | 2002 | 128800 |
| [experience in combination of the educational and scientific work of students]. | 2002 | 127158 | |
| induced transfer of higher plant chloroplasts into fungal protoplasts. | chloroplasts isolated from spinach leaves have been transferred in large numbers into protoplasts of neurospora crassa with the help of polyethylene glycol. the chloroplasts show high photosynthetic activity--at least until the time of uptake--and the protoplasts continue to show active cytoplasmic streaming after chloroplast uptake. | 2002 | 127380 |
| [postoperative esophageal stenosis (role of the laryngologist)]. | 2002 | 153373 | |
| [pneumatization of the paranasal sinuses. causes and symptoms of hypopneumatization]. | 2002 | 127302 | |
| resection of a massive arteriovenous malformation of the head and neck. | we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case. | 2003 | 154679 |
| [50 years of the institute for experimental pathology and therapy in sukhumi]. | 2003 | 148825 | |
| dl-2-keto-4-hydroxyglutarate-1. | 2003 | 165381 | |
| purification of cytochrome b from neurospora crassa and other sources. | 2003 | 152379 | |
| [ivan andreevich spiriukhov (on his 70th birthday)]. | 2003 | 150266 | |
| aeromonas sobria corneal ulcer. | 2003 | 12579184 | |
| solar inactivation of mesophilic aeromonas by exogenous photooxidation in high-rate algal pond treating waste water. | investigations were carried out to observe the effect of sunlight on the survival of mesophilic aeromonas (a. caviae, a. hydrophila and a. sobria) in high-rate algal pond. | 2003 | 12588563 |
| studies of the mechanism of action of the aerolysin-like hemolysin of aeromonas sobria in stimulating t84 cells to produce cyclic amp. | we previously reported that the aerolysin-like hemolysin of aeromonas sobria stimulates t84 cells to produce cyclic amp, which then emerges in the culture medium. in order to clarify the mechanism of action of the hemolysin, we examined the involvement of adenosine nucleotide. the results show that the hemolysin stimulates t84 cells to release atp, which is then converted to adenosine by ectonucleotidase. the adenosine generated might stimulate the p1 adenosine receptors of t84 cells to produce ... | 2003 | 12595476 |
| detection and characterization of the hemolysin genes in aeromonas hydrophila and aeromonas sobria by multiplex pcr. | a multiplex pcr assay was designed to amplify the aeromonas hydrophila and a. veronii bv. sobria hemolysin and aerolysin genes. the assay was evaluated by using 121 clinical isolates and 7 reference strains of aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex pcr. the five genotypes were characterized as type 1 for those carrying the ahh1 gene only (36% of isolates), type 2 for those carrying the asa1 gene only (8.5% of isolates), type 3 for ... | 2003 | 12624028 |
| bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates. | 30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ... | 2003 | 12644237 |
| liver abscess and aeromonas bacteremia with septic pulmonary embolism. | a 68-year-old healthy man was admitted because of liver abscess and aeromonas bacteremia accompanied by pulmonary embolism. aeromonas sobria was isolated from blood. coagulation parameters were normal and a lower limb venogram showed no evidence of thrombosis. septic embolism originating from the liver abscess was considered as the possible cause of pulmonary embolism. the patient was successfully treated with antibiotics. | 2003 | 14606725 |
| microbial transformation of pyrethroid insecticides in aqueous and sediment phases. | recent studies showed that synthetic pyrethroids (sps) can move via surface runoff into aquatic systems. fifty-six of sp-degrading bacteria strains were isolated from contaminated sediments, of which six were evaluated for their ability to transform bifenthrin and permethrin in the aqueous phase and bifenthrin in the sediment phase. in the aqueous phase, bifenthrin was rapidly degraded by strains of stenotrophomonas acidaminiphila, and the half-life (t1/2) was reduced from >700 h to 30 to 131 h. ... | 2004 | 14768859 |
| rapid screening of highly efficient vaccine candidates by immunoproteomics. | diseases caused by microorganisms can be controlled by vaccines, which require neutralizing antigens. therefore, it is very important to identify highly efficient immunogens for immune prevention. by combining immunoproteomics and bacterial challenge after immunization, we developed a rapid method for screening protected antigens of pathogenic bacteria in aquaculture. our approach may be divided into three consecutive steps. first, dominant immunogens of outer membrane proteins are screened by i ... | 2004 | 15378699 |
| universal primer pcr with dgge for rapid detection of bacterial pathogens. | a universal primer pcr (uppcr) combined with denaturing gradient gel electrophoresis (dgge) was evaluated as a method permitting the rapid detection of pathogens. the results show that this method is efficient at amplifying the conserved regions of bacterial 16s rrna genes with universal primers and can detect causative bacterial pathogens rapidly. six species of bacteria from fisheries (pseudomonas fluorescens, vibrio anguillarum, aeromonas hydrophila, vibrio fluvialis, providencia rettgeri and ... | 2004 | 15134888 |
| [purification and immobilization of chondroitinase from aeromonas sobria yh 311]. | chondroitinase has been used as an important tool in the study of the structure, function and distribution of glycosaminoglycans for many years. recently, the enzyme has been reported to be a potential enzyme for chemonucleolysis, an established treatment for intervertebral disc protrasion. in this paper, a chondroitinase had been purified from the culture supernatant of aeromonas sobria yh311 using a simple purification procedure of ammonium sulfate precipitation, qae-sephadex a50 ion exchange ... | 2004 | 15968993 |
| an outbreak of hospital acquired diarrhea due to aeromonas sobria. | six children admitted in a 18 bedded hematology-oncology unit, developed acute diarrhea in a four week period between march and april 2001. salmonella senftenberg was the additional pathogen in the stool sample of one patient who developed cola colored urine and pneumonia in the course of his illness. all the aeromonas strains had a similar biotype and antibiogram. the diarrhea subsided spontaneously in two children whilst three responded to antimicrobial therapy. one patient sought discharge an ... | 2004 | 15475633 |
| effect of high-energy feedings on low birthweight infants. | a comparison of the effect of 100.5 kj/30 ml (24 kcal/30 ml) feedings with that of 84 kj/30 ml (20 kcal/30 ml) feedings on infants with a birthweight of 1500 g or less was made. no consistent benefits in growth occurred and there was a tendency for an increase in complications in infants fed the 100.5 kj/30 ml milk. hence the higher-energy feeding cannot be recommended as the routine feeding for low birthweight infants. | 2004 | 470692 |
| fred jenner hodges, ii, m.d. 1895--1977. | 2004 | 375293 | |
| homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen. | the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ... | 2004 | 387578 |
| [ultrasonic localization for closed renal biopsy]. | 2004 | 520913 | |
| a simple centrifuge column for desalting protein solutions. | 2005 | 543535 | |
| [extracellular nucleases of phytopathogenic bacteria]. | 2005 | 1214627 | |
| geriatric patient care. opinions of a sample of nurses. | 2005 | 1134987 | |
| [points of view on mechanisms of cardiac arrhythmias]. | 2005 | 1770585 | |
| [personality of patients with myocardial infarct]. | 2005 | 1236645 | |
| recognition of individual genes in diverse microorganisms by cycling primed in situ amplification. | cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ... | 2005 | 16269764 |
| use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum). | from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ... | 2005 | 16336470 |
| adaptational changes in cellular fatty acid branching and unsaturation of aeromonas species as a response to growth temperature and salinity. | the effects of growth temperature and salinity on the cellular fatty acids were investigated on aeromonas caviae, aeromonas hydrophila and aeromonas sobria. under optimal growth conditions, fatty acids patterns were dominated by even-numbered chains c(16:0), c(16:1cis9), c(18:1cis11), c(12:0) and c(14:0). growth temperature modifications induced, in the three aeromonas species, important changes in fatty acid (i) unsaturation, (ii) branching and (iii) chain length. an important decrease in the c ... | 2005 | 15925007 |
| a pore-forming toxin produced by aeromonas sobria activates ca2+ dependent cl- secretion. | bacteria produce many types of hemolysin that induce diarrhea by mechanisms that are not completely understood. aeromonas sobria hemolysin (ash) is a major virulence factor produced by a. sobria, a human pathogen that causes diarrhea. since epithelial cells in the intestine are the primary targets of hemolysin, we investigated the effects of ash on ion transport in human colonic epithelial (caco-2) cells. ash increased short-circuit currents (isc) in a dose-dependent manner, and it also activate ... | 2005 | 15797812 |
| a pore-forming toxin produced by aeromonas sobria activates camp-dependent cl- secretory pathways to cause diarrhea. | aeromonas sobria hemolysin (ash) is one of the major virulence factors produced by a. sobria, a human pathogen that causes diarrhea. we investigated the effects of ash on cl(-) transport in human colonic epithelial cells. ash increased short-circuit currents (isc) and (125)i efflux from caco-2 cells, indicating ash activate cl(-) secretion. additions of inhibitors of cyclic amp dependent cl(-) channels, glybenclamide and nppb suppressed the isc and (125)i efflux increases induced by ash. and ash ... | 2005 | 15621437 |
| placental and intestinal alkaline phosphatases are receptors for aeromonas sobria hemolysin. | the aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. we previously identified the putative receptor for a. sobria hemolysin as a p66 protein on intestine 407 cells (microb. pathog. 27 (1999) 215-221). here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (plap). recombinant plap expressed in 293t cells was also found to bind to hemolysin and the binding was ... | 2005 | 15715171 |
| [detection of multiresistance aeromonas with tem type resistant genes in patients with cirrhosis]. | to study the status of beta-lactamase produced by multiresistant aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading. | 2005 | 16201471 |
| antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish. | motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ... | 2005 | 15747443 |
| aeromonas sobria, a causative agent of disease in farmed perch, perca fluviatilis l. | significant numbers of perch, perca fluviatilis, raised on a pilot fish farm in switzerland presented focal skin lesions on the lateral sides and fin rot. mortality rates reached levels of up to 1% of the total fish on the farm per day. virtually pure cultures of aeromonas sobria were isolated from the liver, kidney, spleen and skin lesions of affected fish. aeromonas sobria isolated from the farmed perch had a haemolytic effect on sheep and trout erythrocytes, autoaggregated, was cytotoxic for ... | 2005 | 15752274 |
| aeromonas sobria hemolysin causes diarrhea by increasing secretion of hco3-. | aeromonas sobria hemolysin (ash) is one of the major virulence factors produced by a. sobria, a causative agent of diarrhea in humans. we investigated the effects of ash on anion transport in human colonic epithelial cells. ash increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external hco3-. iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. ... | 2006 | 16630261 |
| cleavage specificity of the serine protease of aeromonas sobria, a member of the kexin family of subtilases. | subtilisin-like proteases have been grouped into six families based on a sequence of the catalytic domain. one of the six is the kexin family, of which furin is a representative protease. all members of the kexin family, except one, are from eukaryotes. the one prokaryotic protease is a serine protease of aeromonas sorbria (asp). here, we examined the substrate specificity of asp based on the cleavage of short peptides. the results showed that asp preferentially cleaves the peptide bond followin ... | 2006 | 16487335 |
| induction of vascular leakage and blood pressure lowering through kinin release by a serine proteinase from aeromonas sobria. | aeromonas sobria causes septic shock, a condition associated with high mortality. to study the mechanism of septic shock by a. sobria infection, we examined the vascular leakage (vl) activity of a. sobria serine proteinase (asp), a serine proteinase secreted by this pathogen. proteolytically active asp induced vl mainly in a bradykinin (bk) b(2) receptor-, and partially in a histamine-h(1) receptor-dependent manner in guinea pig skin. the asp vl activity peaked at 10 min to 1.8-fold of the initi ... | 2006 | 17142774 |
| [a just pension system]. | 2006 | 1636316 | |
| memory and anergy: challenges to traditional models of t lymphocyte differentiation. | traditional paradigms suggest that encounter with an antigen converts naive peripheral t cells into memory cells with less stringent requirements for activation and increased capacities for lymphokine production. recent evidence argues that this view may be over-simplified in two ways. first, an encounter with antigen in the absence of certain costimulatory factors can render a t cell anergic--that is, unable to respond to antigen under normal conditions. second, although cells of the memory t c ... | 2006 | 1563595 |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| aeromonas sharmana sp. nov., isolated from a warm spring. | a gram-negative, facultatively anaerobic bacterial strain designated gptsa-6(t) was isolated from a water sample collected from a warm spring in assam, india. preliminary analysis of the 16s rrna gene sequence of this isolate revealed its affiliation to the family aeromonadaceae. detailed characterization using a polyphasic approach indicated that strain gptsa-6(t) is most closely related to aeromonas sobria but differs significantly from existing members of the genus aeromonas. analysis of the ... | 2006 | 16902029 |
| mechanistic studies on the mononuclear znii-containing metallo-beta-lactamase imis from aeromonas sobria. | in an effort to understand the reaction mechanism of a b2 metallo-beta-lactamase, steady-state and pre-steady-state kinetic and rapid freeze quench electron paramagnetic resonance (epr) studies were conducted on imis and its reaction with imipenem and meropenem. ph dependence studies revealed no inflection points in the ph range of 5.0-8.5, while proton inventories demonstrated at least 1 rate-limiting proton transfer. site-directed mutagenesis studies revealed that lys224 plays a catalytic role ... | 2006 | 16939225 |
| a high containment polymodal pilot-plant fermenter--design concepts. | a 225 dm3 pilot-plant bioreactor system has been designed and constructed that is suitable for biohazardous fermentations. the design enables operation at containment levels above the requirements of good industrial large-scale practice (gilsp) without secondary containment of the whole plant. the main biosafety features of the systems include the use of steam barriers on o-ring seals, supply lines and stirrer seals, multiple o-ring seals, piping of condensate lines and pressure relief systems t ... | 2006 | 1367227 |
| aeromonas hydrophila and aeromonas sobria bacteremia: rare pathogens of infection in a burn patient. | 2007 | 17084982 | |
| aeromonas sobria prostatitis and septic shock in a healthy man with chronic alcoholic consumption. | prostate infection caused by aeromonas sobria has not been reported in the literature. we presented a 44-year-old healthy man with a habit of alcoholic consumption who was admitted because of primary a. sobria prostatitis with septicemia. the patient was successfully treated with intravenous ofloxacin and was uneventful after a half-year follow-up. based on our rare case, we suggest to clinical physicians that prostatitis might be considered in healthy male patients with a. sobria bacteremia and ... | 2007 | 18032845 |
| activation of prothrombin by asp, a serine protease released from aeromonas sobria. | the effect of a serine protease (asp) secreted from aeromonas sobria on plasma coagulation was investigated. proteolytically active asp promoted human plasma coagulation in a dose-dependent manner. consistent with the preference for a factor xa-specific oligo-peptide substrate, asp produced enzymatic activity from human prothrombin but not from factors ix and x. asp cleaved prothrombin to produce enzymatically active 37 kda-fragment displaying the same molecular mass as alpha-thrombin. asp is th ... | 2007 | 18067862 |
| mediator and cellular mechanisms in asthma. the philip ellman lecture 1990. | 2007 | 2258848 | |
| induction of apoptosis in vero cells by aeromonas veronii biovar sobria vacuolating cytotoxic factor. | recently, a cytotoxin named vacuolating cytotoxic factor (vcf) in aeromonas sobria and aeromonas veronii biovar sobria was described. we have now purified this factor using ion metallic affinity chromatography. the vcf is a nonhemolytic enterotoxin that acts as a serine protease. the toxin can be partially neutralized by serum antiaerolysin and it induced not only cytoplasmatic vacuole formation, but also mitochondrial disorders that must be signaling the apoptotic pathways, leading to vero (afr ... | 2007 | 17286562 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| the immune response and protective efficacy of oral alginate microparticle aeromonas sobria vaccine in soft-shelled turtles (trionyx sinensis). | aeromonas sobria is an important bacterial pathogen of soft-shelled turtles (trionyx sinensis). the effect of an oral alginate microparticle a. sobria vaccine (mav) on immune response and protection efficacy of soft-shelled turtles was investigated. the formalin-killed cultures of a. sobria strain z-1 (fkc) were incorporated into alginate microparticles, and the encapsulated vaccine was orally used for immunizing turtles. the serum agglutinating antibody titers, bactericidal activity of blood le ... | 2007 | 17628697 |
| the immune response and protective efficacy of vaccination with oral microparticle aeromonas sobria vaccine in mice. | the effect of an oral alginate microparticle aeromonas sobria vaccine on immune response and protection of mice was investigated. the formalin-killed cultures (fkc) of a. sobria strain z-1 treated with three methods were incorporated into alginate microparticles, and the encapsulated vaccines were orally used for immunizing mice. four weeks after immunization, the serum agglutinating antibody titers, bactericidal activity of leucocytes and relative percent survival (rps) of mice immunized orally ... | 2007 | 17630205 |
| rapid detection of virulence factors of aeromonas isolated from a trout farm by hexaplex-pcr. | the detection of virulence factors of aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. in this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and aeromonas species were isolated and identified. for rapid detection of six virulence factors of isolated aeromonas, a hexaplex-polymerase chain reaction (hexaplex-pcr) assay was used. the detected virulence factors include a ... | 2007 | 17846582 |
| molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein. | lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ... | 2007 | 17850883 |
| salt in surroundings influences the production of serine protease into milieu by aeromonas sobria. | previously we have shown that the open reading frame 2 protein (orf2 protein), which is encoded at the 3 ' end of serine protease of aeromonas sobria (asp), functions as a chaperone protein in periplasm in the production of asp. both proteins, asp and orf2 protein, associate in periplasm and orf2 protein helps asp to take an active form. asp which is dissociated from orf2 protein emerges in milieu . in this study, we examined the effect of sodium chloride (nacl) in medium on asp production by a. ... | 2007 | 17951986 |
| clinical significance and distribution of putative virulence markers of 116 consecutive clinical aeromonas isolates in southern taiwan. | the objectives of this study were to elucidate the clinical manifestations of aeromonas infections and the association of putative virulence genes with clinical invasiveness. | 2007 | 16716402 |
| the development of probiotics for the control of multiple bacterial diseases of rainbow trout, oncorhynchus mykiss (walbaum). | jb-1 and gc2, which were equated with bacillus sp. and aeromonas sobria respectively, were recovered from the digestive tract of rainbow trout, oncorhynchus mykiss and ghost carp, cyprinus sp. respectively, and demonstrated effectiveness as probiotics for the control of infections caused by aeromonas salmonicida, lactococcus garvieae, streptococcus iniae, vibrio anguillarum, vibrio ordalii and yersinia ruckeri. when administered to rainbow trout (average weight = 12 g) for 14 days in feed dosed ... | 2007 | 17850573 |
| identification of bacteria from the normal flora of perch, perca fluviatilis l., and evaluation of their inhibitory potential towards aeromonas species. | pathogenic aeromonas sobria has been identified as a causative agent of ulcerative disease in farmed european perch, perca fluviatilis l. to study the effect of the normal intestinal bacterial flora of perch against a. sobria, we sampled 193 bacterial isolates from the perch digestive tract. the isolates were identified by sequence analysis of the 16s rrna gene and their inhibitory potential against a. sobria was evaluated in vitro. nineteen of the strains isolated showed inhibition and were als ... | 2008 | 18355180 |
| [characterization of aeromonas spp isolates from newborns hospitalized]. | aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ... | 2008 | 18545840 |
| proteomic analysis of rainbow trout (oncorhynchus mykiss, walbaum) serum after administration of probiotics in diets. | the response of rainbow trout (oncorhynchus mykiss, walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (apr). proteomic analysis by two-dimensional electrophoresis (2d) concurrently with mass spectrometry was used to detect apr related proteins in rainbow trout serum following feeding with probiotics aeromonas sobria gc2 and bacillus sp. jb-1. three candidate proteins increased following use of gc2, and wer ... | 2008 | 17981340 |
| cystic neoplasms of the pancreas. | eight patients with cystic neoplasms of the pancreas were seen at four northern california hospitals between the years 1978 and 1986. three of the tumors were benign and five were malignant. three females, whose average age was 61 years, had cystadenomas. three females and two males, whose average age was 48 years, had mucinous cystadenocarcinomas. clinical presentations were similar among all patients. abdominal pain was a prominent feature. anorexia, weight loss, nausea and vomiting with a pal ... | 2008 | 2446398 |
| impaired plasma clottability induction through fibrinogen degradation by asp, a serine protease released from aeromonas sobria. | aeromonas sobria infection often advances to sepsis, in which interaction of bacterial components with plasma proteins possibly causes various disorders. this bacterium releases a serine protease (asp), a putative virulence factor, and binds to fibrinogen. to study the asp effect on fibrinogen, we incubated fibrinogen or plasma with asp and investigated their clotting elicited by thrombin, which converts fibrinogen to a fibrin clot. enzymatically active asp retarded plasma clotting in a dose-dep ... | 2008 | 18462393 |
| fluid secretion caused by aerolysin-like hemolysin of aeromonas sobria in the intestines is due to stimulation of production of prostaglandin e2 via cyclooxygenase 2 by intestinal cells. | to clarify the mechanisms of diarrheal disease induced by aeromonas sobria, we examined whether prostaglandin e2 (pge2) was involved in the intestinal secretory action of a. sobria hemolysin by use of a mouse intestinal loop model. the amount of pge2 in jejunal fluid and the fluid accumulation ratio were directly related to the dose of hemolysin. the increase over time in the level of pge2 was similar to that of the accumulated fluid. in addition, hemolysin-induced fluid secretion and pge2 synth ... | 2008 | 18086811 |
| production of c5a by asp, a serine protease released from aeromonas sobria. | aeromonas sobria causes pus and edema at sites of infection. however, the mechanisms underlying these effects have not been elucidated. c5a, the amino-terminal fragment of the complement 5th component (c5), mimics these events. to investigate the involvement of c5a in the pathophysiology of a. sobria infection, we examined release of c5a from human c5 by a serine protease (asp), a putative virulence factor secreted by this bacterium. c5 incubated with enzymatically active asp induced neutrophil ... | 2008 | 18714034 |
| efficacy of in-feed probiotics against aeromonas bestiarum and ichthyophthirius multifiliis skin infections in rainbow trout (oncorhynchus mykiss, walbaum). | the aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by aeromonas bestiarum and ichthyophthirius multifiliis (ich) in rainbow trout. | 2008 | 18422955 |
| toxin production by aeromonas sobria in natural environments: river water vs. seawater. | aeromonas are water-borne pathogens. they are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% nacl). however, the presence of aeromonas in seawater is extremely rare compared with that in river water. in this study, we tested the ability of aeromonas sobria to produce toxins in river water and seawater. first, we cultured a. sobria on skim milk agar plates supplemented with either river water (sarw) or seawater (sasw). the ... | 2008 | 19122681 |
| non-germ cell testicular tumours in nigerians. | non-germ cell testicular tumours are reported to be more common in peoples of negroid and asian descent than in caucasians. this group of tumours comprised seven of sixteen testicular tumours seen in a ten-year period at the university of benin teaching hospital, benin city, nigeria. however, unlike experiences elsewhere in africa and the west indies where paratesticular tumours accounted for a majority of these tumours, malignant lymphomas constituted over 70% of non-germinal testicular tumours ... | 2008 | 2635452 |
| non-germ cell testicular tumours in nigerians. | non-germ cell testicular tumours are reported to be more common in peoples of negroid and asian descent than in caucasians. this group of tumours comprised seven of sixteen testicular tumours seen in a ten-year period at the university of benin teaching hospital, benin city, nigeria. however, unlike experiences elsewhere in africa and the west indies where paratesticular tumours accounted for a majority of these tumours, malignant lymphomas constituted over 70% of non-germinal testicular tumours ... | 2008 | 2635452 |
| a randomized trial of anticoagulation with warfarin and of alternating chemotherapy in extensive small-cell lung cancer by the cancer and leukemia group b. | the cancer and leukemia group b (calgb) conducted a prospective randomized trial to evaluate the role of warfarin and alternating chemotherapy in extensive small-cell lung cancer (sccl). after stratification for sex and performance status, patients were randomly assigned to receive chemotherapy with methotrexate, doxorubicin (adriamycin; adria laboratories, columbus, oh), cyclophosphamide, and lomustine (ccnu) (macc), or macc plus warfarin (macc + w), or mitomycin, etoposide, cisplatin, and hexa ... | 2008 | 2547030 |
| enrolled nurses. we're backing the charter! | 2008 | 2587373 | |
| repeated hepatic ischemia as a treatment for carcinoid liver metastases. | hepatic ischemia has been used in the treatment of bilobar malignant carcinoid tumors in the liver. hepatic artery ligation, hepatic dearterialization, and embolization with nondegradable micromaterial have been followed by collateral formation and are associated with a high complication rate. to reduce this, an implantable vascular occluder permitting intermittent occlusion of the hepatic artery was used in 3 patients with bilateral malignant carcinoid in the liver, with high urinary excretion ... | 2009 | 2741468 |
| more on index of refraction. | 2009 | 2805311 | |
| more on index of refraction. | 2009 | 2805311 | |
| monoclonal antibodies as carriers of toxins. | 2009 | 2785694 | |
| structural basis for the kexin-like serine protease from aeromonas sobria as sepsis-causing factor. | the anaerobic bacterium aeromonas sobria is known to cause potentially lethal septic shock. we recently proposed that a. sobria serine protease (asp) is a sepsis-related factor that induces vascular leakage, reductions in blood pressure via kinin release, and clotting via activation of prothrombin. asp preferentially cleaves peptide bonds that follow dibasic amino acid residues, as do kex2 (saccharomyces cerevisiae serine protease) and furin, which are representative kexin family proteases. here ... | 2009 | 19654332 |
| a novel method for the diagnosis of drowning by detection of aeromonas sobria with pcr method. | the acid digestion method has been widely used for the diagnosis of death by drowning, but it is not always sensitive. however, there has been no definitive method to replace acid digestion until now. we speculate that bacteria are more useful markers than plankton for the diagnosis of death by drowning. in this study, from the preserved blood samples of 32 freshwater drowning cases, specific dna fragments of aeromonas sobria, one of the most common aquatic bacteria, were examined using pcr. the ... | 2009 | 19766051 |
| the carboxy-terminal tail of aeromonas sobria serine protease is associated with the chaperone. | asp is the only bacterial protease in the kexin group of the subtilisin family. previous studies have revealed that the orf2 protein encoded at the 3' end of the asp operon is required for asp to change from a nascent form into an active form in the periplasm. however, the mechanism by which orf2 makes contact and interacts with asp in the maturation process remains unknown. the present study examined the effect of mutations in the carboxy-terminal region of asp on the asp maturation process. bo ... | 2009 | 19954452 |
| rosmarinus officinalis l.: chemical modifications of the essential oil and evaluation of antioxidant and antimicrobial activity. | rosmarinus officinalis essential oil was separated into its hydrocarbon and oxygenated fractions. the major compounds in the hydrocarbon fraction were alpha-pinene (44.2%), camphene (24.5%), and limonene (11.7%), while in the oxygenated fraction they were 1,8-cineole (37.6%), camphor (16.5%), and bornyl acetate (21.4%). the hydrocarbon fraction was submitted to a hydroformylation process and the antioxidant activity of the product was screened by the dpph and beta-carotene/linoleic acid tests. t ... | 2009 | 20120107 |
| clinical presentations, prognostic factors, and mortality in patients with aeromonas sobria complex bacteremia in a teaching hospital: a 5-year experience. | bacteremia due to aeromonas sobria complex is an uncommon clinical presentation, associated with a high mortality rate. this retrospective study reviewed the clinical manifestations and prognostic factors of a. sobria complex bacteremia. | 2009 | 20422137 |
| case reports: fatal necrotizing fasciitis caused by aeromonas sobria in two diabetic patients. | we report two rare cases of aeromonas sobria necrotizing fasciitis with sepsis in patients with diabetes. in both cases, immediate fasciotomy was performed and appropriate empiric antimicrobial therapy and intensive care were administered. however, the two patients died on day 2 and day 11, respectively, after admission as a result of multiple organ failure. when patients present with a rapid onset of skin necrosis and progressive sepsis, an aeromonas sobria infection or vibrio infection should ... | 2009 | 18800212 |
| appendicitis associated with travelers' diarrhea caused by aeromonas sobria. | aeromonas species is an infrequent pathogen causing travelers' diarrhea and gastroenteritis. no human case of appendicitis has been reported as a complication of aeromonas infection until now. we describe a case of acute appendicitis associated with aeromonas sobria infection acquired in cebu, philippines. | 2009 | 19335814 |
| aeromonas sobria sepsis complicated by rhabdomyolysis in an hiv-positive patient: case report and evaluation of traits associated with bacterial virulence. | human infection with aeromonas species is uncommon and most often due to trauma with exposure to contaminated water or soil. a 43-year-old hiv- and hepatitis c virus (hcv)-infected male, after a two-week course of corticosteroid therapy for an autoimmune anemia, developed diarrhea, dermatologic manifestations and a multiple organ dysfunction syndrome, resulting in death. although stool samples were repeatedly negative, two sets of blood cultures obtained during a single peak of fever yielded the ... | 2009 | 18977680 |
| lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
| pseudomonas chlororaphis strain jf3835 reduces mortality of juvenile perch, perca fluviatilis l., caused by aeromonas sobria. | motile aeromonad septicaemia caused by aeromonas sobria is a cause of disease in farmed perch, perca fluviatilis l., in switzerland. we have evaluated the potential of a pseudomonas chlororaphis isolate, obtained from perch intestine, to control a. sobria infection. inoculation of juvenile perch with p. chlororaphis strain jf3835 prior to infection with a. sobria caused a reduction in a. sobria associated mortalities. infection of perch with xyle-labelled p. chlororaphis indicated the bacterium ... | 2009 | 19476558 |
| association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of anodonta cygnea (linnaeus, 1758). | bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. a complete understanding of the balance between anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of a. cygnea collected from mira lagoon in northern portugal. the results showed vibrio metschnikovii and aeromonas sobria as the dominant groups. th ... | 2010 | 20349058 |