Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer. | autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ... | 1987 | 3679544 |
| differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor. | motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative. | 1987 | 3611325 |
| plasmids and resistance to antimicrobial agents in aeromonas sobria and aeromonas hydrophila clinical isolates. | the antimicrobial susceptibilities of 75 aeromonas isolates were determined by agar dilution. differences in resistance patterns were observed between strains isolated from different geographic locations and between a. sobria and a. hydrophila isolates. multiple resistance was common; however, only one conjugative plasmid was detected. this 110-megadalton plasmid mediated resistance to eight antibiotics. | 1987 | 3631947 |
| a practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity. | a known ornithine decarboxylase assay working with ion exchange separation of [3h]ornithine and [3h]putrescine has been revised. the assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. the separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. the detection limit is about 50 pmoles [3h]putrescine formed. | 1987 | 3545892 |
| determination of antimony levels in organs of mice, treated with an antimony-albumin complex, by atomic absorption spectroscopy. | 1987 | 3609261 | |
| placental amino acid uptake in normal and complicated pregnancies. | amino acids are the essential substrates for fetal growth and catabolism. the fetus is dependent on the placenta for the provision of amino acids, the first step being concentration of amino acids within the syncytiotrophoblast for subsequent transfer to the fetus. a reliable technique for the isolation of human syncytiotrophoblast plasma membrane has been described, and the suitability of this preparation for the study of amino acid uptake and membrane transport has been well documented. using ... | 1988 | 3354595 |
| evaluation of prostaglandins as mediators of tubuloglomerular feedback. | orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ... | 1988 | 3364575 |
| an unusual case of pyogenic meningitis due to aeromonas sobria. | 1988 | 3382842 | |
| induction of lyme arthritis in lsh hamsters. | in studies of experimental lyme disease, a major obstacle has been the unavailability of a suitable animal model. we found that irradiated lsh/ss lak hamsters developed arthritis after injection of borrelia burgdorferi in the hind paws. when nonirradiated hamsters were injected in the hind paws with b. burgdorferi, acute transient synovitis was present. a diffuse neutrophilic infiltrate involved the synovia and periarticular structures. the inflammation was associated with edema, hyperemia, and ... | 1988 | 3410540 |
| roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo. | fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ... | 1988 | 3044171 |
| stimulation of rabbit synoviocyte prostaglandin e2 synthesis by lipopolysaccharides and their subunit structures. | lipopolysaccharides (lps) induce synoviocyte activation and may lead to destruction of synovial joint tissues. we assessed the production of prostaglandin e2 (pge2) as a measure of synoviocyte activation by lps and their subunit structures. diphosphoryl lipid a was the smallest portion of lipid a tested that stimulated pge2 production. the polysaccharide fraction of lps, containing the o antigen, was also active. intraarticular injections of the polysaccharide resulted in a synovitis very simila ... | 1988 | 3162365 |
| aeromonas-associated gastroenteritis in children. | during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ... | 1988 | 3340460 |
| gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae. | clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ... | 1988 | 3366862 |
| nucleotide sequence and transcriptional analysis of the aercaera region of aeromonas sobria encoding aerolysin and its regulatory region. | the nucleotide sequence of a 2510 base pair chromosomal fragment containing the aerolysin gene aera, and its regulatory region aerc, from a clinical isolate of aeromonas sobria was determined. the aerolysin gene coded for a 54.5 kd polypeptide and had a g + c content of 59%, indicating that it is endogenous to the genus aeromonas. in contrast, the aerc region was characterized by its high a + t content (61%) and the presence of a core motif, aataaaa, repeated eight times within 300 base pairs. a ... | 1988 | 2459581 |
| biochemical and toxigenic characteristics of aeromonas spp. isolated from diseased mammals, moribund and healthy fish. | in this study we describe biochemical, toxigenic and surface characteristics of 33 motile aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. aeromonas hydrophila, a. caviae and a. sobria were isolated from both mammals and fish but at a different incidence. aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, catt ... | 1988 | 3188376 |
| enteropathogenicity of aeromonas species isolated from infants: a cohort study. | the significance of aeromonas spp. as potential enteric pathogens was evaluated in a cohort of 187 infants aged 3-18 months during a 16-week summer period. aeromonas spp. were isolated from 14 of the 196 (7.1%) diarrhoeal episodes detected and from eight (5.2%) of 153 samples from paired asymptomatic infants. carriage of bacterial enteropathogens excluding aeromonas spp. was detected in a high proportion (23%) of the asymptomatic children. almost all of the seven isolates of aeromonas sobria wer ... | 1988 | 3216131 |
| [toxic properties of lipopolysaccharide and lipid a of aeromonas sobria and non 01 vibrio cholerae]. | 1988 | 3216765 | |
| [immunogenic and antigenic properties of bacterial cells, lipopolysaccharides and lipids a of aeromonas sobria and non 01 vibrio cholerae]. | 1988 | 3216766 | |
| carbon substrate assimilation patterns of clinical and environmental strains of aeromonas hydrophila, aeromonas sobria and aeromonas caviae observed with a micromethod. | the assimilation of carbon substrates by 103 strains of aeromonas of different origin identified by conventional methods was studied by means of a standardized micromethod containing 147 tests (api system). six distinct groups could be recognized and the discriminating substrates were determined. 3 species of aeromonas can be identified by means of conventional method: a. hydrophila, a. sobria and a. caviae. the method has a number of drawbacks: some media are unreliable, others are difficult to ... | 1988 | 3218392 |
| [serological cross reactions of lps and lipid a isolated from aeromonas sobria and non 01 vibrio cholerae]. | 1988 | 3246916 | |
| [characteristics of immunoglobulins specific for lps and lipid a of aeromonas sobria and non 01 vibrio cholerae]. | 1988 | 3246917 | |
| mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis. | in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ... | 1988 | 2838023 |
| biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah. | an epizootic of feline infectious peritonitis in a captive cheetah population during 1982-1983 served to focus attention on the susceptibility of the cheetah (acinoyx jubatus) to infectious disease. subsequent observations based upon seroepidemiological surveys and electron microscopy of fecal material verified that cheetahs were indeed capable of being infected by coronaviruses, which were antigenically related to coronaviruses affecting domestic cats, i.e. feline infectious peritonitis virus/f ... | 1988 | 2849387 |
| isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin. | shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ... | 1988 | 3045003 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets. | the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ... | 1989 | 2556355 |
| concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated. | because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ... | 1989 | 2561041 |
| histamine concentration and ca2+ mobilization in arterial smooth muscle. | smooth muscle contraction is dependent on ca2+ entry from the extracellular space or release from intracellular stores. the sensitivity of these ca2+ sources to agonist concentration was evaluated by measuring myoplasmic [ca2+] (as estimated by aequorin), myosin phosphorylation, and isometric stress in the swine carotid media. high histamine concentrations produced transient elevations in [ca2+] and phosphorylation with rapid generation of near maximal stress. lower histamine concentrations prod ... | 1989 | 2750885 |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| comparative sequence analysis, in vitro expression and biosynthesis of mouse prp. | a number of studies have indicated that an endogenous brain protein, prp, is associated with transmissible agents causing spongiform encephalopathies such as scrapie, kuru, and creutzfeldt-jakob disease. it has been proposed that prp derived from scrapie brain is the scrapie agent itself. to test directly whether the prp mrna in scrapie brain tissue can encode the scrapie agent, we expressed prp cdna cloned from scrapie-infected mouse brain in vitro. the expressed prp did not transmit scrapie to ... | 1989 | 2574873 |
| prp (proline-rich protein) genes linked to markers es-12 (esterase-12), ea-10 (erythrocyte alloantigen), and loci on distal mouse chromosome 6. | the closely linked proline-rich protein (prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. they are part of a conserved linkage group that is represented on human chromosome 12p. two other markers, ea-10 and es-12, that were previously unassigned to a chromosome are closely linked to prp genes in the mouse. | 1989 | 2613230 |
| mutational specificity and the influence of excision repair: insights into the mechanisms of error-avoidance and error-fixation. | the excision repair process controlled by the uvrabc gene in escherichia coli is the major pathway for the repair of a diverse series of dna damages. to achieve a better understanding of the mechanics of this repair pathway and its impact upon mutagenesis, we have applied a recently developed technology by which the nature of mutation is determined at the dna sequence level. a comparison of the classes and distribution of mutation in excision-repair-proficient and excision-repair-deficient strai ... | 1989 | 2698833 |
| [antibacterial and bactericidal activities of japanese green tea]. | we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ... | 1989 | 2677434 |
| [experimental study of the role of male ixodid ticks in the circulation of the tick-borne encephalitis virus]. | regular transmission of the tick-borne encephalitis virus by ixodidae males feeding on laboratory animals is demonstrated. the virus titres in the male saliva were 0.1-1.5 lgld50/0.03 ml. it was established that such doses didn't produce tense viremia in white mice or clinical tick-borne encephalitis. | 1989 | 2779496 |
| production and characterization of monoclonal antibodies to aeromonas sobria surface antigens. | a panel of eight murine monoclonal antibodies (mabs) was raised against the surface antigens of aeromonas sobria isolated from a patient with diarrhoea. antibodies to the lps core and o-antigen and to protein antigens were generated. three mabs of the igm isotype against either protein or lps agglutinated 34/75 aeromonas isolates from clinical and environmental sources. similar numbers of a. hydrophila, a. sobria and a. caviae isolates were agglutinated. the mabs were screened for their ability ... | 1989 | 2483346 |
| [intestinal infections caused by aeromonas]. | we have identified 38 instances of intestinal infection by aeromonas and 4 by plesiomonas in 16,184 coprocultures carried out during a 4 year period in the hcu of zaragoza. the low prevalence can be related with the fact that specific media for the recovery of these organisms were not used. the species aeromonas sobria (47.36%) and a. caviae (39.47%) were more common than a. hydrophila (13.15%). aeromonas were isolated from patients with and without intestinal disease. the occurrence of gastroin ... | 1989 | 2490673 |
| antimicrobial susceptibility of sixty human fecal isolates of aeromonas species. | the mics of 21 antimicrobial agents were determined for 60 strains of aeromonas spp. isolated from human feces. all isolates tested were susceptible to aztreonam, tetracycline, imipenem, moxalactam, pipemidic acid, gentamicin, trimethoprim-sulfamethoxazole, pefloxacin and ciprofloxacin. resistance to erythromycin and streptomycin was observed in all 60 strains. aeromonas caviae was less susceptible to cefamandole, cefotaxime, norfloxacin, chloramphenicol, tetracycline, sulfamethoxazole and trime ... | 1989 | 2496995 |
| bacterial growth and toxin production in ileostomy effluents. | escherichia coli (2), vibrio cholerae (2) and aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. the patterns of response ... | 1989 | 2649680 |
| fever response in laboratory-maintained and free-ranging chuckwallas (sauromalus obesus). | the fever response was studied in laboratory-maintained and free-ranging chuckwallas. five animals were used in a self-pairing laboratory experiment in which each animal was first injected with a sterile, pyrogen-free saline solution. approximately 7 days later, each individual was injected with a solution of 4.75 x 10(9) aeromonas sobria (formerly a. hydrophila). saline-injected individuals had a mean preferred body temperature (mpbt) of 36.8 +/- 0.4 (se) degrees c, whereas bacteria-injected an ... | 1989 | 2750958 |
| characterization of aeromonas sobria hemolysin by use of monoclonal antibodies against aeromonas hydrophila hemolysins. | aeromonas sobria produces hemolysin in a form activable with trypsin under defined cultural conditions. in immunoblotting analyses with the culture supernatant of a. sobria, the monoclonal antibody reacting specifically to aeromonas hydrophila ca-11 hemolysin bound to the 53,000- and 49,000-dalton bands before and after trypsinization, respectively. the monoclonal antibody reacting to a. hydrophila ah-1 hemolysin did not bind either band. a. sobria hemolysin is, therefore, related antigenically ... | 1989 | 2768466 |
| species identification of aeromonas strains based on carbon substrate oxidation profiles. | twenty clinical strains each of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria were evaluated for their abilities to oxidize one or more of 95 carbon sources on a gn microplate (biolog, hayward, calif.). nine substrates yielded good, discriminatory values for the three species tested. the panel appears to be useful for the species identification of aeromonas isolates originating from human material. | 1989 | 2778077 |
| [the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. | 1989 | 2806660 | |
| [spectroscopy of the liver in vivo using nuclear magnetic resonance. a new approach in hepatic physiopathology]. | nuclear magnetic resonance (nmr) spectroscopy is a non-invasive means of detecting numerous compounds, thus enabling real-time studies to be carried out on such topics as energy metabolism, intracellular ph and main intermediate metabolism pathways. this method has been used for several years to study perfused livers and rat livers in vivo, but its application to man has just begun. we describe some of its principal uses, with emphasis on the possibilities it offers to investigate the metabolic ... | 1990 | 2140172 |
| uptake and retrograde transport of [3h]gaba from the cochlear nucleus to the superior olive in the guinea pig. | the purpose of the present study is to determine which descending projections to the cochlear nucleus may use gamma-aminobutyric acid (gaba) as a neurotransmitter. [3h]gaba (120 microm) was injected into the cochlear nucleus of albino and pigmented guinea pigs. after survival times between 0.25 and 16 h, the brain stems were prepared for light microscopic autoradiography. after 2 h survival there was a pulse of label, which progressed through the fibres from the cochlear nucleus to the ipsilater ... | 1990 | 1697753 |
| aeromonas sobria in bubaline (water buffalo) abortion: growth requirement, biochemical characters, antibiotic susceptibility, experimental pathogenicity and serology. | a. sobria was isolated unexpectedly on campylobacter selective medium from abortion specimens of 16 buffaloes. all strains (as1-as16) required campylobacter growth supplement (sodium pyruvate, sodium metabisulphate and ferrous sulphate) and 10% c02 atmosphere on primary isolation. they were unusually sensitive to bile salt and failed to grow on macconkey's agar. biochemically, these strains were homogeneous but antibiogram profile and physiological/other characters revealed a moderate heterogene ... | 1990 | 2351419 |
| microbiologic and clinical evidence supporting the role of aeromonas caviae as a pediatric enteric pathogen. | aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were isolated once each. the infants from whom a. caviae was isolated all presented with a watery diarrhea lasting 1 to 3 weeks. none of these infants was breast-fed, and all had a stool ph of greater than 7.5. all of the a. caviae isolates, including a reference strain (at ... | 1990 | 2351730 |
| in vitro susceptibility of aeromonas caviae, aeromonas hydrophila and aeromonas sobria to fifteen antibacterial agents. | in vitro testing of the activity of 15 antibacterial agents against 522 clinical isolates of aeromonas species demonstrated some species-associated trends. amoxicillin plus clavulanic acid was effective against approximately 45% of aeromonas caviae and aeromonas hydrophila, but all aeromonas sobria isolates were resistant. aztreonam, piperacillin and mezlocillin were highly active against all the strains of aeromonas tested. ticarcillin was equally effective against aeromonas caviae and aeromona ... | 1990 | 2387294 |
| dynamics of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae in a sewage treatment pond. | the spatiotemporal dynamics of aeromonas spp. and fecal coliforms in the sewage treatment ponds of an urban wastewater center were studied after 20 months of sampling from five stations in these ponds. isolation and identification of 247 aeromonas strains were undertaken over four seasons at the inflow and outflow of this pond system. the hemolytic activity of these strains was determined. the aeromonas spp. and the fecal coliform distributions showed seasonal cycles, the amplitude of which incr ... | 1990 | 2389929 |
| the incidence of virulence factors in mesophilic aeromonas species isolated from farm animals and their environment. | sixty-one isolates of aeromonas spp. from the faeces of pigs, cows and a variety of associated environmental sources were examined for the characteristics that are reputed to have roles in pathogenicity. most isolates of aeromonas hydrophila were cytotoxic (96.4%) and were capable of producing cell elongation factor (75%) and haemagglutinins (67.9%). in contrast few of the aeromonas caviae isolates produced these three markers (13.6%, 27.3% and 36.4% respectively). in general, aeromonas sobria o ... | 1990 | 2209733 |
| direct selection of monoclonal antibodies neutralising the cytotoxic activity of aeromonas sobria. | monoclonal antibodies directed against the cytotoxic activity of aeromonas sobria were raised by immunising mice with a culture supernatant concentrated by ammonium sulphate precipitation. neutralising antibodies were specifically selected for by exposing hybridomas to cytotoxic levels of the immunising preparation. cultures free from cytopathic effects after three hours were selected for further investigation. ten cytotoxin resistant hybridomas were isolated but only two of these produced detec ... | 1990 | 2272497 |
| aerolysin of aeromonas sobria: evidence for formation of ion-permeable channels and comparison with alpha-toxin of staphylococcus aureus. | aerolysin from aeromonas sobria ab3 was isolated and purified. the pure toxin formed sodium dodecyl sulfate-insoluble oligomers in a lipidic environment. the addition of aerolysin to the aqueous phase bathing lipid bilayer membranes resulted in the formation of ion-permeable channels which had a single-channel conductance of about 70 ps in 0.1 m kcl. this defines the toxin as a channel-forming component similar to other toxins but without any indication for an association-dissociation reaction, ... | 1990 | 1694819 |
| effect of bacterial pyrogen on three lizard species. | 1. three lizard species (callopistes maculatus, gerrhosaurus major, and varanus exanthematicus) were tested for their response to intraperitoneal injection of alcohol-killed aeromonas sobria. 2. a paired experimental design, in which each animal received an injection of sterile saline and 1 x 10(10) a. sobria, was utilized. 3. c. maculatus demonstrated a statistically significant increase in mean selected body temperature (msbt) after bacteria injection. 4. g. major and v. exanthematicus did not ... | 1990 | 1976479 |
| analysis of the autoagglutination phenomenon, serogroup and hydrophobicity as virulence markers in mesophilic aeromonads isolated from stool cultures. | the autoagglutination phenomenon (aa) was studied in 71 strains of mesophilic aeromonads isolated from stool cultures of patients with acute diarrhea (28 aeromonas hydrophila, 20 aeromonas sobria, and 23 aeromonas caviae). two phenotypes were considered in the aa+ strains: sp+ (self-pelleting) and pab+ (precipitation after boiling). serogrouping of the strains was performed only with the 0:11 serum. other properties studied were agglutination with acriflavine (acr) and crystal violet uptake (cvb ... | 1990 | 2073902 |
| [new enteropathogens: aeromonas spp]. | aeromonas is one of the type of enteric pathogens which is commonly present in less developed countries. it leads to acute diarrhea in children and traveller's diarrhea in adults. additionally it is also responsible from the extraintestinal infections. aeromonas hydrophila is a bacteria which is isolated from water, soil, foods and rarely from intestinal tract of human. aeromonas sobria is especially isolated from wound infection of scuba diver's. there are other types of aeromonas such as salmo ... | 1990 | 2089235 |
| induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis. | 1990 | 2164982 | |
| isolation of aeromonas spp. from canal water. | aeromonas sobria was identified from soil and canal water at the east end of london. there was a correlation between water and faecal isolates from children attending the nearby children's hospital. in view of the potential risk of enteric and non-enteric infections due to aeromonas spp, efforts are needed to ensure that canals, rivers, natural water sources and domestic water supply are free of these organisms. | 1990 | 2361702 |
| mechanisms of inhibition of various cellular dna and rna polymerases by several flavonoids. | four flavonoids (i.e., baicalein, quercetin, quercetagetin, and myricetin), known to be inhibitors of hiv-reverse transcriptase, have been shown to be more or less inhibitory to the activities of various cellular dna and rna polymerases. the degree of the inhibition varied depending on the combination of the flavonoid and the enzyme species: baicalein was moderately inhibitory to dna polymerase gamma and e. coli dna polymerase i; quercetin was strongly inhibitory to dna polymerase beta and e. co ... | 1990 | 2292590 |
| assay of all-trans----11-cis-retinoid isomerase activity in bovine retinal pigment epithelium. | 1990 | 2292965 | |
| pathogenicity factors and virulence for rainbow trout (salmo gairdneri) of motile aeromonas spp. isolated from a river. | ninety-seven motile aeromonas strains were isolated over a period of a year from samples of water and sediment collected at different sites along a river. strains were regularly recovered from all samples, regardless of the source of isolation or seasonal conditions. isolates were biochemically characterized by the api 20ne system (analytab products, plainview, n.y.) and classified as aeromonas hydrophila (74 strains), aeromonas sobria (11 strains), and aeromonas caviae (12 strains). despite the ... | 1990 | 2312678 |
| pyrazinamidase activity as a phenotypic marker for several aeromonas spp. isolated from clinical specimens. | negative pyrazinamidase activity was significantly associated with aeromonas sobria, and positive pyrazinamidase activity was associated with a. hydrophila and a. caviae (chi 2, p less than 0.0001). the absence of pyrazinamidase activity may be a potentially significant phenotypic marker for a. sobria. | 1990 | 2312687 |
| spinal cord repair: is tissue oxygenation an important variable? | we have demonstrated the reliability and feasibility of making pto2 recordings from graft and host tissue in the injured spinal cord. the data suggest that the oxygen microenvironment of developing graft and host spinal tissue is clearly different from that found in normal spinal tissue or in transplants that have not survived or integrated well. these same constraints seem to apply to cavitation in developing grafts and poorly developed graft/host interfaces. the similarity between these findin ... | 1990 | 2096642 |
| rapid presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity. | a new test combination for presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity in conjunction with esculin hydrolysis and gas production from glucose was evaluated. of 40 clinical isolates of aeromonas tested statically at 30 degrees c, seven were identified as aeromonas hydrophilia (non-suicidal, aerogenic, esculin positive), eight as aeromonas sobria (suicide variable, mostly aerogenic, esculin negative), and 25 as aeromonas caviae (suicidal, anaer ... | 1990 | 2209629 |
| electrophoretic analysis of the surface components of autoagglutinating surface array protein-positive and surface array protein-negative aeromonas hydrophila and aeromonas sobria. | the protein and lipopolysaccharide (lps) compositions of 10 autoagglutinating aeromonas hydrophila and aeromonas sobria strains were studied; one group consisted of five serogroup o:11 strains that contained an s layer, while a second group was composed of diverse serogroups that were s layer negative by transmission electron microscopy. all serogroup o:11 strains were found to contain a predominant 52,000- to 54,000-molecular-weight protein that was present on both whole-cell and outer membrane ... | 1990 | 2229347 |
| cytostatic and cytotoxic effects of recombinant tumor necrosis factor-alpha on sensitive human melanoma cells in vitro may result in selection of cells with enhanced markers of malignancy. | monolayer cultures of the human melanoma cell lines stml-12, stml-11, stml-14 (third, respectively, twenty-fifth subculture), and skmel-28 derived from specimens representing different stages of tumor progression were treated with 10-10,000 u/ml rtnf-alpha applied for 72 h. the effects of rtnf-alpha on cell proliferation, dna synthesis, cell viability, cloning efficiency, cell division, cell morphology, and the immunophenotype were studied in triplicate experiments. the cell line stml-14(3) reve ... | 1990 | 2258639 |
| cytostatic and cytotoxic effects of recombinant tumor necrosis factor-alpha on sensitive human melanoma cells in vitro may result in selection of cells with enhanced markers of malignancy. | monolayer cultures of the human melanoma cell lines stml-12, stml-11, stml-14 (third, respectively, twenty-fifth subculture), and skmel-28 derived from specimens representing different stages of tumor progression were treated with 10-10,000 u/ml rtnf-alpha applied for 72 h. the effects of rtnf-alpha on cell proliferation, dna synthesis, cell viability, cloning efficiency, cell division, cell morphology, and the immunophenotype were studied in triplicate experiments. the cell line stml-14(3) reve ... | 1990 | 2258639 |
| hemoglobin e and glucose-6-phosphate deficiency in the khmer air force (cambodia). | this study was designed to investigate the use of primaquine in malaria control in the khmer republic (cambodia). blood was drawn from 106 male khmer air force troops for analysis of hemoglobin e and glucose-6-phosphate (g-6-pd) deficiency. a test group of 15 men with g-6-pd deficiency and a group of 31 normal controls were given 15 mg of primaquine each morning for 14 days. the patients were followed for hemoglobinuria and changes in hematocrit. primaquine induced a significant, but not a dange ... | 1990 | 268155 |
| financial management of department of nursing services. | 1990 | 258786 | |
| primary papillary carcinoma of the prostate: report of a histopathologic, cytologic and electron microscopic study on one case. | an unusual type of prostatic carcinoma is described. histopathologic examination showed a papillary and acinar appearance. aspiration cytology showed papillary fragments and sheets of malignant cells. the ultrastructural features of this tumor were similar to those reported for prostatic carcinoma rather than endometrial carcinoma supporting a prostatic orgin for this tumor. | 1990 | 269605 |
| reflection on geriatrics. | 1990 | 267819 | |
| [effect of anaprilin on the clinical course of stenocardia and the carbohydrate metabolic indices of ischemic heart disease patients in relation to the nature of their glucose tolerance]. | fourteen-day anaprilin treatment of coronary patients with varying glucose tolerance has demonstrated that the drug's therapeutic efficiency was greater in patients with normal glucose tolerance. anaprilin caused a moderate drop in baseline glucose level and considerably limited the degree of hyperglycemia in response to the glucose tolerance test in coronary patients with abnormally low glucose tolerance. insulin content was virtually unaffected by the drug, irrespective of the type of glucose ... | 1991 | 3327995 |
| dermoid cyst of the floor of the mouth in an infant. (case report). | dermoid cysts of the floor of the mouth in the infant are rarely encountered although the aetiology is mainly embryological. meyer proposes the classification of the cysts based on the histological differentiation of the elements of the cyst wall. a case is reported which shows differences in the epithelial lining of the cysts found followed by the differential diagnosis and treatment. | 1991 | 269895 |
| experimental evidence for toxin production by aeromonas hydrophila and aeromonas sobria in a meat extract at low temperatures. | the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (enterotoxin and haemolysin) in a meat extract at low temperatures (5 and 12 degrees c) was investigated. all three strains incubated at 12 degrees c were enterotoxigenic and haemolytic in the meat extract after 5 days. at 5 degrees c, five of the six strains tested were able to produce these exotoxins after 8 days incubation while one strain was neither enterotoxigenic nor haemolytic after 5 ... | 1991 | 2049284 |
| evaluation of the antimicrobial activity of methylglyoxal bis(guanylhydrazone) analogues, the inhibitors for polyamine biosynthetic pathway. | metabolic and antiproliferative effects of methylglyoxal bis(butylamidinohydrazone) (mgbb) and methylglyoxal bis(cyclopentylamidinohydrazone) (mgbcp), inhibitors for polyamine biosynthetic pathway, on escherichia coli, shigella sonnei, aeromonas sobria, aeromonas hydrophila and vibrio cholerae were investigated. mgbb at the concentration of 100 mumol/l depleted intracellular putrescine and spermidine concentrations of e. coli to 25 and 20% of the controls, respectively, while mgbcp depressed the ... | 1991 | 2055790 |
| aeromonas spp. and their association with human diarrheal disease. | between january 1988 and december 1989 aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. no other bacterial enteric pathogens were found in any of these 45 patients. of the 45 aeromonas isolates, 35 strains (77.8%) were aeromonas hydrophila, 7 (15.5%) were aeromonas sobria, and 3 (6.7%) were aeromonas caviae. most of the patients were under 5 years of age. no bacterial enteric pathogens, including aeromonas species, were isolated from 512 age- and sex-m ... | 1991 | 2056050 |
| haemolysin occurrence among aeromonas hydrophila, aeromonas caviae and aeromonas sobria strains isolated from different aquatic ecosystems. | a total of 909 aeromonas spp. isolates from different aquatic ecosystems were tested for haemolysin production by both sheep and horse blood agar-plate assays and by rabbit erythrocytes in broth assay. a comparison of these different methods was undertaken in order to appreciate their capacity to evaluate the haemolytic activity of aeromonas spp. isolated from aquatic ecosystems. the haemolytic activity was associated particularly with a. hydrophila and a. sobria (about 95% of strains), whereas ... | 1991 | 2068383 |
| [severe occurrence of idiopathic ulcerative colitis caused by aeromonas sobria]. | 1991 | 1910924 | |
| aeromonas hydrophila: variability in biochemical characteristics of environmental isolates. | a total of 161 strains, including 144 aeromonads, of which 100 had been isolated from water and sediment samples from the chesapeake bay, five clinical and 33 anacostia river isolates of aeromonas hydrophila, six aeromonas sobria, and ten reference cultures were examined in a numerical taxonomy study in which each of the strains were examined for 56 unit characters. similarity between strains was computed using the simple matching (ssm) and jaccard (sj) coefficients. the strains were clustered b ... | 1991 | 1920081 |
| gas-liquid chromatographic analysis of cellular fatty acid methyl esters in aeromonas species. | the cellular fatty acids of 39 strains belonging to the genus aeromonas (aeromonas hydrophila, aeromonas caviae, aeromonas sobria, aeromonas media, aeromonas schubertii, aeromonas veronii) were determined by high resolution gas-liquid chromatography. the fatty acid profiles were characterized by major amounts (60% or more) of one saturated (hexadecanoic acid = 16:0) and two unsaturated (hexadecenoic acid = 16:1 and octadecenoic acid = 18:1) acids. while the majority of the strains of the six spe ... | 1991 | 1930556 |
| a positive fever response in agama agama and sceloporus orcutti (reptilia: agamidae and iguanidae). | three species of lizards (agama agama, mabuya perrotetii, and sceloporus orcutti) were tested for a possible increase in mean selected body temperature (msbt) in response to intraperitoneal injection of alcohol-killed aeromonas sobria, a gram-negative bacterium known to be pathogenic to reptiles. a paired experimental design was utilized in which each animal was given an injection of sterile saline and 1.10(10) a. sobria. body temperatures were monitored via indwelling cloacal thermocouples at 4 ... | 1991 | 1939744 |
| intestinal secretory immune response to infection with aeromonas species and plesiomonas shigelloides among students from the united states in mexico. | intestinal secretory iga (siga) response or lack of response among adults in mexico with diarrhea was used as an indicator of enteropathogenicity of aeromonas species and plesiomonas shigelloides. siga was extracted from stool specimens obtained at day of presentation and 5 days later. total siga was standardized, and specific siga titer against the organism being shed by each patient was determined. western blotting was used to determine which microbial antigens elicited an intestinal siga resp ... | 1991 | 1940478 |
| purification and characterization of aeromonas sobria pili, a possible colonization factor. | pili of aeromonas sobria ae1 were purified and characterized. the molecular mass of the pilin was estimated to be about 23 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the ae1 pili were electrophoretically and immunologically distinguishable from the w pili of a. hydrophila ae6, although the two pili were morphologically indistinguishable. the n-terminal amino acid sequences of the two pilins were identical in the first 10 residues. strain ae1 and its purified pili adhered t ... | 1991 | 1680105 |
| isolation and characterization of a cryptic plasmid from mesophilic aeromonads: potential as a cloning vector. | a 2.6 kb covalently closed circular plasmid has been isolated from clinical and environmental isolates of aeromonas sobria and a. hydrophila. the possibility that the plasmid carries genetic determinants that mediate resistance to a variety of anti-microbial agents has been eliminated. the plasmid is stable at approximately 20-25 copies per chromosome equivalent which, together with its relatively small size and the presence of unique restriction sites, makes it a good candidate for development ... | 1991 | 1366999 |
| growth of and toxin production by aeromonas hydrophila and aeromonas sobria at low temperatures. | the effects of different temperatures on the growth and toxin production of aeromonas hydrophila and aeromonas sobria were studied. the results showed that these aeromonas species are not only able to grow at low temperatures (e.g. at 4 and 10 degrees c) but may also produce cytotoxin, hemolysin and enterotoxin under suitable growth conditions. | 1991 | 1888660 |
| biochemical and genetic characterization of autoagglutinating phenotypes of aeromonas species associated with invasive and noninvasive disease. | the genetic characteristics and biochemical and structural properties of a number of autoagglutinating (aa) strains of aeromonas associated with invasive and noninvasive disease in humans and infections in animals and from environmental sources were investigated. of 27 strains analyzed by multilocus enzyme typing and dna hybridization studies, 25 (93%) were confirmed to belong to either hybridization group 1 (phenospecies and genospecies aeromonas hydrophila) or 8 (phenospecies aeromonas sobria; ... | 1991 | 2010642 |
| structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6. | we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ... | 1991 | 1828805 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| aeromonas sobria spontaneous bacterial peritonitis. | 1992 | 1449170 | |
| [late onset serum iga and igm deficiency revealed by acute diarrhea caused by aeromonas sobria infection]. | 1992 | 1526403 | |
| enterotoxic effects of aeromonas sobria haemolysin in a rat jejunal perfusion system identified by specific neutralization with a monoclonal antibody. | investigations into the pathogenesis of aeromonas diarrhoea have demonstrated that several different cell-free products of motile aeromonads show enterotoxic activity in suckling mouse, rat, and rabbit assay systems. the relative contributions made by separate cytotoxic and cytotonic activities in the mixture produced by in vitro culture remains unresolved. using a modified rat jejunal perfusion assay, we have studied the effects of a. sobria culture filtrates containing defined levels of haemol ... | 1992 | 1564437 |
| molecular studies on the aerolysin gene of aeromonas species and discovery of a species-specific probe for aeromonas trota species nova. | a large group of aeromonads and other enteric microorganisms were assayed for the presence of the aerolysin gene with use of dna-dna hybridization. two dna fragments corresponding to the regulatory region (aerc) and the structural gene (aera) were used as probes for the detection of the aerolysin gene in these strains. sequences corresponding to the aerolysin structural gene were widespread among aeromonas isolates. in contrast, the aerc probe was much more selective, and sequences corresponding ... | 1992 | 1600007 |
| [the occurrence of aeromonads in a drinking water supply system]. | this study concerns with the occurrence of aeromonads, coliforms and colony counts in a drinking water supply. aeromonas contents were detected in the range of 15.0 to greater than 2,400/100 ml in the raw water samples of the man made lake. after the drinking water treatment process including fast sand filtration and chlorination aeromonads indicated in comparison to total coliforms and colony counts early and significant an after-growth of maximal 240 aeromonads/100 ml in the peripheric drinkin ... | 1992 | 1609555 |
| rapid detection of members of the family enterobacteriaceae by a monoclonal antibody. | six monoclonal antibodies directed against enterobacteria were produced and characterized. the specificity of one of these antibodies (cx9/15; immunoglobulin g2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. all of the enterobacteria were specifically recognized, the only exception being erwinia chrysanthemi (one strain tested). bacteria not belonging to members of the family enterobacteriaceae were not detected, except for ... | 1992 | 1622220 |
| application of ribotyping for differentiating aeromonads isolated from clinical and environmental sources. | we have investigated the usefulness of ribotyping for the differentiation of aeromonads isolated from five patients with gastroenteritis and from the source water, treatment plant, and distribution system of a small public water supply. aeromonas hydrophila and aeromonas caviae were isolated from fecal specimens preserved in cary-blair transport medium by using blood ampicillin agar or alkaline peptone water (ph 8.4) subcultured to blood ampicillin agar plates. a. hydrophila, aeromonas sobria, a ... | 1992 | 1622269 |
| nucleotide sequences and characterization of haemolysin genes from aeromonas hydrophila and aeromonas sobria. | extracellular haemolysin is thought to be one of the important virulence factors in aeromonas infection. two extracellular haemolysin genes (ahh3 and ahh4) from aeromonas hydrophila strain 28sa, one (ahh5) from a. hydrophila strain ah-1 and one (asa1) from aeromonas sobria strain 33 were cloned into cosmid and plasmid vector dna in escherichia coli. the nucleotide sequences of the open reading frames of ahh3 and ahh4 are both 1476 basepairs (bp), whereas ahh5 and asa1 are 1455 and 1467 bp in len ... | 1992 | 1302284 |
| characterization of aeromonas sobria tap13 pili: a possible new colonization factor. | pili of aeromonas sobria tap13 were purified and characterized. the molecular mass of the pilin was estimated to be about 23 kda by sds-page. the tap13 pili were immunologically different from a. sobria ae1 pili and a. hydrophila ae6 w pili as previously reported, nevertheless all three had indistinguishable morphology and shared a high degree of homology in their n-terminal amino acid sequences. strain tap13 and its purified pili did not agglutinate human, rabbit or sheep erythrocytes. however, ... | 1992 | 1357078 |
| purification and characterization of aeromonas sobria ae24 pili: a possible new colonization factor. | pili of aeromonas sobria ae24 were purified and characterized. the molecular mass of the pilin was estimated to be about 19 kda by sds-page. the ae24 pili were electrophoretically distinguishable from previously reported aeromonas hydrophila ae6 w pili and a. sobria ae1 pili, although all three had indistinguishable morphology and shared a high degree of homology in the n-terminal amino acid sequences. strain ae24 and its purified pili adhered to rabbit intestine and agglutinated human and rabbi ... | 1992 | 1363704 |
| binding of laminin and fibronectin by the trypsin-resistant major structural domain of the crystalline virulence surface array protein of aeromonas salmonicida. | the surface of aeromonas salmonicida is covered by a tetragonal paracrystalline array (a-layer) composed of a single protein (a-protein, mr = 50,778). this array is a virulence factor. cells containing a-layer and isolated a-layer sheets specifically bound laminin and fibronectin with high affinity. binding by cells was inactivated by selective removal of a-layer at ph 2.2, and neither isogenic a-layer-deficient a. salmonicida mutants nor tetragonal paracrystalline array producing aeromonas hydr ... | 1992 | 1730607 |
| [camp factor for the differentiation among aeromonas species]. | the camp factor technique, described by natale figura as a presumptive method for the differentiation of the species hydrophila, sobria and caviae, was applied to 80 aeromonas strains isolated from children under 5 years with acute diarrheic disease. the typical phenomenon was seen in aerobiosis and anaerobiosis conditions in 10 strains, which were classified as aeromonas hydrophila. in aerobiosis conditions/alone, it was observed in 20 strains, which were identified as aeromonas sobria. it was ... | 1992 | 9768204 |
| el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens]. | a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ... | 1993 | 7984811 |
| [the identification of aeromonas species isolated in cuba]. | 100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests. | 1993 | 7984818 |