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the estimation of serum creatine phosphokinase activity using a reaction-rate analyser. 20154581093
[the origins of huttonian thought: hutton, student and physician]. 20134629584
propoxyphene. two fatalities due to overdose. 20144684062
giardia intestinalis. a prospective investigation of the prevalence in denmark. 19734697076
temporal and contextual cues as discriminative attributes in retardates' recognition memory. 20154704210
cystic fibrosis and coeliac disease. report of two cases. 20154733638
uptake and effects of melatonin on the synthesis of proteins by the rat cerebral cortex. 19734766259
[closed fracture of the hyoid bone body with the formation of the false joint]. 20164767991
[the formation of subjective evaluation of time in man]. 19734772236
the maximum derivatives of left ventricular pressure and transverse internal diameter as indices of the inotropic state of the left ventricle in conscious dogs.1. in normal, conscious dogs, i.v. infusion of isoprenaline caused inases in heart rate and the maximal derivatives of left ventricular pressure (dp/dt max) and left ventricular internal diameter (dd/dt max). the changes in both derivatives were linearly related to the increase in heart rate.2. increments in heart rate produced by right atrial pacing caused only minimally significant increases in both dp/dt max and dd/dt max at heart rates of 180 beats/min. increases in heart rate, with end dias ...19734772400
[fatal pedestrian-accidents in street traffic (author's transl)]. 20154774662
different patterns of induction of the two isozymes of alanine aminotransferase of liver of rat as a function of age. 19744826465
preventing and detecting leakage in epidural catheters. 20164856942
[application of isotopes to heart-pulmonary circulation dynamics]. 20164871482
modern practice in immunization. 10. tetanus immunization. 20164884415
resonance energy transfer in pepsin conjugates. 20164897805
microangiopathic hemolytic anemia. 20164913945
degradation products and the role of coagulation in "persistent" glomerulonephritis. 20164931741
sudden death from neurological causes in india. 20155025982
association of aeromonas sobria with human infection.fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...19816112237
enterotoxic activity of aeromonas hydrophila and aeromonas sobria. 19836421948
distribution of imvc biogroups of aeromonas hydrophila and aeromonas sobria isolated from human, fish and water.aeromonas sobria and a.hydrophila were isolated from infected fish in ratio of 3.5:1 during the outbreak of fish infections from december 1982 to february 1983, while isolates from human diarrheic stool was 1 :2. on the basis of imvc reactions 138 isolates of motile aeromonads could be divided into 11 biogroups, with biogroup 4 showing statistically significant association with infections. nine biogroups of aeromonads which were isolated from infected fish reflected that the outbreak was not cau ...19836658502
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...19846690467
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...19846699147
cholera-like illness due to aeromonas sobria.a thai woman from bangkok was admitted to a hospital in paris for a cholera-like illness. a culture of her "rice-water" diarrhea was negative for vibrio cholerae and enterotoxigenic escherichia coli but was positive for aeromonas sobria. this strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. the enterotoxin, which was labile at 100 c ...19827054327
a toxigenic profile of aeromonas hydrophila and aeromonas sobria isolated from fish.forty strains of motile aeromonas were isolated from healthy and diseased fish. these strains were identified as a. hydrophila or a a. sobria. it was found that only strains of a. hydrophila produced a dermonecrotic factor and two zones of hemolysis on blood agar. all the strains of a. sobria tested and 72% of the a. hydrophila were enterotoxigenic. finally, we observed that only a. hydrophila strains could regularly produce hemolysin at 10 degrees c.19817237279
serogrouping of motile aeromonas species isolated from healthy and moribund fish.a total of 195 strains of motile aeromonas isolated from fish were characterized as aeromonas hydrophila and aeromonas sobria. in view of the frequency of isolation and the importance of motile aeromonas species as fish pathogens, a serological classificaton of these organisms was attempted. antisera were prepared in rabbits against formalinized whole cell suspensions and against boiled cells of 12 different isolates. seventy-six strains could be grouped by tube agglutination with whole cells as ...19817259164
isolation, enumeration, and characterization of aeromonas from polluted waters encountered in diving operations.counts of total viable, aerobic, heterotrophic bacteria, indicator organisms, and aeromonas spp. were made at a diver training site on the anacostia river in washington, d.c. the numbers of aeromonas cells in anacostia river sediment and water increased during periods of elevated water temperature, to maxima of 4 x 10(5) cells per g of sediment and 300 cells per ml of water. correspondingly, aeromonas counts dropped 2 to 4 logs as the water temperature decreased to 0 to 0.5 degrees c. cultures t ...19807396482
variable expression of o-antigen and the role of lipopolysaccharide as an adhesin in aeromonas sobria.on initial isolation of aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767o) and translucent (3767t). strain 3767o consistently produced lipopolysaccharide (lps) core and o-antigen side chain, detectable by sds-page and by western blotting with an o-antigen-specific monoclonal antibody. strain 3767t produced lps core but the amount of o-antigen was dependent on factors including growth medium and bacterial growth phase. strain 3767t exhibited sig ...19947525403
a clinical isolate of aeromonas sobria with three chromosomally mediated inducible beta-lactamases: a cephalosporinase, a penicillinase and a third enzyme, displaying carbapenemase activity.hydrolytic profiles of aeromonas sobria 163a, non-induced and induced, and of a derepressed mutant 163a-m, indicate that this strain has inducible beta-lactamases with activities against cephalosporins, penicillins and carbapenems. three enzymes were identified and two of the beta-lactamase genes, amps, encoding a penicillinase and ceps, encoding a cephalosporinase, were cloned into escherichia coli, permitting analysis of the individual enzymes. isoelectric focusing (ief) analysis using inhibit ...19957759391
cloning and expression of a cloxacillin-hydrolyzing enzyme and a cephalosporinase from aeromonas sobria aer 14m in escherichia coli: requirement for an e. coli chromosomal mutation for efficient expression of the class d enzyme.two beta-lactamase genes, asba1 and asbb1, encoding asba1 and asbb1, respectively, have been cloned from aeromonas sobria aer 14m into escherichia coli. asba1 was expressed at low but detectable levels in all e. coli laboratory cloning strains tested. asbb1 was expressed well in the e. coli cloning strain dh5 alpha. however, no enzyme activity could be detected from the same clone when placed in e. coli mc1061. ampicillin-resistant mutants of e. coli mc1061 were obtained that expressed high leve ...19947811022
isolation and characterization of the prolyl aminopeptidase gene (pap) from aeromonas sobria: comparison with the bacillus coagulans enzyme.the aeromonas sobria pap gene encoding prolyl aminopeptidase (pap) was cloned. it consists of 425 codons and encodes a homotetrameric enzyme of 205 kda. the purified enzyme showed an almost absolute specificity for amino-terminal proline. proline and hydroxyproline residues from many peptide and amide substrates could be easily removed, while no activity was detected for substrates having other amino terminals. the enzyme was very similar to that from bacillus coagulans in many aspects, such as ...19947883756
prolyl aminopeptidase is not a sulfhydryl enzyme: identification of the active serine residue by site-directed mutagenesis.prolyl aminopeptidase (pap) has been classified as a sulfhydryl enzyme on the basis of its high sensitivity to p-chloromercuribenzoate and heavy metals. recently, however, the possibility of pap being instead a serine enzyme has been raised as a result of two observations--the conservation of some residues among the paps hitherto sequenced, and a similarity to some serine hydrolases. this is the first report describing an attempt to identify the active residue by site-directed mutagenesis. the p ...19947896753
el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens].a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ...19937984811
[the identification of aeromonas species isolated in cuba].100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests.19937984818
aeromonas peritonitis.five new cases of peritonitis caused by aeromonas species are reported, and 29 others described in the literature are reviewed. males predominated (71%), and the mean age was 56.9 years. acquisition was nosocomial in 20% of the cases. all patients except one (3%) had significant underlying diseases; 73% had chronic hepatic disease, 15% had chronic renal failure (treated with chronic ambulatory peritoneal dialysis [capd]), and 9% had an intestinal perforation. symptoms were similar to those of pe ...19948054435
the effect of bacteria infection on mean selected body temperature in the common agama, agama agama: a dose-response study.1. the fever response was studied in 43 common agamas using a self-pairing experiment in which animals received an intraperitoneal injection of sterile saline and an injection of one of six dosages of dead aeromonas sobria (1 x 10(6), 1 x 10(7), 1 x 10(8), 1 x 10(9), 1 x 10(10), and 1 x 10(11) total organisms). 2. the results demonstrated a significant increase in tb (1.6-3.1 degrees c) above the mean selected body temperature (msbt) of the saline injection animals over a bacteria infection rang ...19938101779
wound infection due to aeromonas sobria. 19938110950
characterization of mesophilic aeromonas from clinical specimens by computerized analysis of sds-page protein profiles and by enzymatic activity.mesophilic aeromonas (aeromonas hydrophila, aeromonas sobria, aeromonas caviae) have recently been considered important aetiological agents of human diseases, mainly gastrointestinal infections. although several findings have pointed out the significance of this group of microorganisms as enteric pathogens and suggested the presence of virulence factors, epidemiological and clinical studies are limited by the difficulty of correctly identifying mesophilic aeromonas at the species level. sds-page ...19938264423
distribution of mesophilic aeromonas species in raw and ready-to-eat fish and meat products in switzerland.a total of 829 poultry, meat, shellfish and fish products commonly consumed in switzerland were qualitatively and quantitatively examined for the presence of mesophilic aeromonas spp. overall, aeromonads occurred in 24.1% of the samples. raw food products were frequently contaminated (e.g. 94.1% in minced meat), with colony counts up to 6.0 x 10(6)/g. some ready-to-eat products had a relatively high percentage of positive samples as well, such as cooked ham in slices (38.2%), mortadella (12.9%), ...19938268055
aeromonas sobria spontaneous bacterial peritonitis and bacteremia. 19948304329
cloning and characterization of three hemolysin genes from aeromonas salmonicida.two hemolysin genes (ash3 and ash4) of aeromonas salmonicida strain 17-2 and one (ash1) of a. salmonicida atcc14174 were cloned into the cosmid vector charomid 9-36 in escherichia coli dh1. the overall amino acid sequence of the ash3 was similar to that of the aerolysins of aeromonas hydrophila and aeromonas sobria, and hemolysins ahh3, ahh4, and ahh5 of a. hydrophila, and hemolysin asa1 of a. sobria. the sequence of ash4 was similar to that of the ahh1 hemolysin of a. hydrophila. the ash4 hemol ...19938309354
[acute gastroenteritis from aeromonas sobria and pancreatitis: apropos of a case]. 19938332027
inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish.the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ...19938357253
isolation and purification of aeromonas sobria cytotonic enterotoxin and beta-haemolysin.aeromonas sp., grown in tryptone soya broth supplemented with yeast extract, 0.6%, ph 7.5, and incubated with agitation at 100 oscillations/min for 15 h at 37 degrees c produced optimal amounts of beta-haemolysin and cytotonic enterotoxin. more prolonged incubation resulted in the loss of enterotoxic activity and anion exchange chromatographic analysis indicated the presence of a moiety capable of breaking down the toxin. anion exchange fast protein liquid chromatography resulted in a single pea ...19938384263
spectrum of extraintestinal disease due to aeromonas species in tropical queensland, australia.during a 12-month period, the clinical spectrum of extraintestinal disease due to aeromonas species was determined for 56 patients in tropical queensland (australia). forty-six patients acquired their infection in the community, six patients were infected in the hospital, and four patients were colonized. demographic risk factors included male gender (67%) and aboriginal ethnic background (35%). the disease ranged from deep-seated infection (four cases) to soft-tissue infection of varying intens ...19938513068
sequence analysis of two chromosomally mediated inducible beta-lactamases from aeromonas sobria, strain 163a, one a class d penicillinase, the other an ampc cephalosporinase.two beta-lactamase genes from aeromonas sobria, strain 163a, have been cloned and sequenced, one encoding a typical class c cephalosporinase, designated ceps, the other a class d penicillinase, designated amps. ceps is predicted to be a mature protein of 38 kda with a pi value of 7.0. the amino acid sequence of ceps is most similar to that of ampc from pseudomonas aeruginosa (54.7%). amps is predicted to be a mature protein of 27 kda with a pi value of 7.9 that mostly closely resembles blad from ...19958537283
aeromonas sobria infection with severe muscle degeneration in a patient with alcoholic liver cirrhosis.a 56-yr-old male who had been followed for alcoholic liver disease was admitted for abdominal pain and a high fever. gastrointestinal endoscopy revealed bleeding esophageal varices that were treated by endoscopic sclerotherapy. blood culture on admission was positive for aeromonas sobria. then skin bullas and ulcers and severe muscle degeneration developed. the patient died despite extensive treatment with antibiotics. a. sobria infection in patients with liver cirrhosis is rare.19958540527
studies on bacteriocinogenic lactobacillus isolates from selected nigerian fermented foods.ten bacteriocin-producing (bacteriocinogenic) lactobacillus isolates obtained from three nigerian fermented foods namely: kenkey, ogi and wara were tested against the following indicator organisms: lactobacillus plantarum and food borne pathogens comprising enterotoxigenic escherichia coli, enterohaemorrhagic escherichia coli, serratia, pseudomonas, vibrio cholerae, aeromonas sobria, aeromonas cavice, salmonella typhimurium, plesiomonas shigelloides and staphylococcus aureus. all the bacteriocin ...19958568643
growth and exotoxin production at low temperatures by aeromonas strains in meat slurries.the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (haemolysin and enterotoxin) and exoenzymes (protease and lipase), as well as their growth in meat slurries stored at low temperatures (5 and 12 degrees c) was investigated. the results showed that aeromonas grew well in meat slurries stored at low temperatures in the presence of background flora. exotoxins and exoenzymes were not detected in filtrates from meat slurries inoculated with enter ...19968643030
endotoxin in aeromonas sobria infection: speculative pathogenesis and treatment strategies in advanced liver disease with soft-tissue infection. 19968678032
[bacteriological studies of travellar's diarrhoea. 5) analysis of enteropathogenic bacteria at osaka airport quarantine station from january 1992 through september 3rd, 1994].during the last 2 years and 8 months before the closure of osaka airport quarantine station (from jan. 1992 to sep. 3, 1994), a total of 7,421,909 overseas travellers were quarantined. 15,919 reported themselves of suffering from diarrhoea. bacteriological examination of a total of 6,031 individuals' stools were performed. 1) various enteropathogenic bacteria were isolated from 31.2% of the stools examined. isolated species were as follows: plesiomonas shigelloides, 1,127 cases (59.9%); vibrio p ...19968822051
prolyl aminopeptidase is also present in enterobacteriaceae: cloning and sequencing of the hafnia alvei enzyme-gene and characterization of the expressed enzyme.the hafnia alvei prolyl aminopeptidase gene (hpap) was cloned and sequenced, the expressed enzyme (hpap) was purified to homogeneity and thoroughly characterized. an open reading frame of 1,281 bp was found to code for the enzyme, resulting in a protein of 427 amino acids with a molecular weight of 48,577. hpap resembles the aeromonas sobria enzyme, having 45% identity and the same distinctive properties with respect to size and substrate specificities. both enzyme show similar chromatographic b ...19968830041
hlya hemolysin of vibrio cholerae o1 biotype e1 tor. identification of the hemolytic complex and evidence for the formation of anion-selective ion-permeable channels.hemolysin (hlya) was concentrated from supernatants of different vibrio cholerae o1 biotype e1 tor strains by ammonium sulfate precipitation. the concentration of the toxin in the supernatants and in the precipitates was quantified using its hemolytic activity. the toxin formed a high molecular-mass band (about 220 kda) on sds/page while the toxin monomer had a molecular mass of 60 kda when it was heated. the addition of the e1 tor hemolysin oligomers, but not that of the monomers, to the aqueou ...19968856066
near-drowning-associated aeromonas pneumonia.aeromonas is increasingly recognized as a human pathogen that causes a variety of different infections. aeromonas has rarely been reported as a cause of respiratory infection, and it has been described in near-drowning-associated pneumonia. this article reviews a case of aeromonas sobria pneumonia associated with a near drowning and considers the clinical and epidemiological characteristics of 10 previously reported cases. nearly all of the cases involved young healthy men, a rapid development o ...19968969997
biochemical characterization of the carbapenem-hydrolyzing beta-lactamase asbm1 from aeromonas sobria aer 14m: a member of a novel subgroup of metallo-beta-lactamases.asbm1, a carbapenem-hydrolyzing beta-lactamase produced by aeromonas sobria aer 14m, was purified chromatographically, with anion exchange chromatography performed in the absence of zn2+. the molecular mass of asbm1 was approximately 34,000; the isoelectric point was 9.1. asbm1 had high hydrolytic specificity for carbapenems but low hydrolysis rates for penicillins and cephalosporins. asbm1 was resistant to the commercially available beta-lactamase inhibitors but was inhibited by pcmb and the ch ...19968998985
[rapid detection of the hemolysin genes in aeromonas sobria by the polymerase chain reaction].the hemolysin of aeromonas sobria is one of the important virulence factors in this organism. rapid detection and identification test for a. sobria is important for early and specific diagnosis of this infectious disease. we evaluated the polymerase chain reaction (pcr) for the rapid detection of a. sobria. two pairs of synthetic oligonucleotide primers (asa1-s and a; aeraas-s and a) were used in pcr technique to detect the different hemolysin genes (asa1 and aeraas) in a. sobria. the pcr identi ...19969011120
[endophthalmitis caused by aeromonas sobria]. 19969011213
expression of the asba1, oxa-12, and asbm1 beta-lactamases in aeromonas jandaei aer 14 is coordinated by a two-component regulon.aeromonas jandaei aer 14 (formerly aeromonas sobria aer 14) expresses three inducible beta-lactamases, asba1, oxa-12 (asbb1), and asbm1. mutant strains that constitutively overexpress all three enzyme simultaneously, suggesting that they share a common regulatory pathway, have been isolated. detectable expression of the cloned genes of asba1 and oxa-12 in some escherichia coli k-12 laboratory strains is achieved only in the presence of a blp mutation. these mutations map to the cre operon at 0 m ...19979068648
[severe acute gastroenteritis due to aeromonas in a patient colectomized for crohn's disease].we report a case of severe acute diarrhoea produced by aeromonas sobria, a specimen with controversial human pathogenicity. a 26-year-old man with crohn's disease previously colectomized was admitted with a cholera-like clinical presentation and renal failure. aeromonas sp. can be isolated in both children and adults and in healthy people, a. hydrophila being most often detected. severe infections due to other aeromonas sp., usually a. sobria and a. caviae, have been rarely described. most patie ...19979090982
aeromonas bacteremia in patients with hematologic diseases.over a 23-year period, 17 patients with hematologic diseases developed aeromonas bacteremia while in our hematology ward. male predominance (14 patients, 82%) was seen, with a predilection for the elderly. hematologic malignancies, especially acute leukemia, accounted for 15 (88%) of all patients. cancer chemotherapy and neutropenia (15 patients each) were the most common preceding host conditions. aeromonas bacteremia generally occurred in the second half of the year (july-december), with no ex ...19979144007
evaluation of caribbean strains of macrocyclops and mesocyclops (cyclopoida:cyclopidae) as biological control tools for the dengue vector aedes aegypti.fifteen caribbean strains of copepods were assessed for their predation ability against mosquito larvae. macrocyclops albidus from nariva. mesocyclops aspericornis from oropouche, and mesocyclops longisetus from e1 socorro, trinidad, were most effective against aedes aegypti but not against culex quinquefasciatus. mesocyclops longisetus and me. aspericornis prevented any mosquito survival over 25 wk of observation despite weekly challenges with ae. aegypti. the copepods were tolerant to dosages ...19979152870
enzyme kinetics and biochemical analysis of imis, the metallo-beta-lactamase from aeromonas sobria 163a.the metallo-beta-lactamase from aeromonas sobria 163a, imis, was isolated in a two stage purification procedure using protein affinity columns. enzyme kinetics show that imis hydrolyses the carbapenems but displays poor activity against other beta-lactams. imis possesses the narrowest spectrum of activity of the group 3 enzymes that have been analysed. sequencing of the 40 n-terminal amino acids show this region to be identical to that of the cpha metallo-beta-lactamase from aeromonas hydrophila ...19969182099
purification and characterization of lipase from aeromonas sobria lp004.lipase from aeromonas sobria lp004, isolated from raw milk, was purified and characterized. the lipase was purified 10.29 fold to a homogeneous state by ultrafiltration and column chromatography on phenyl sepharose. the molecular weight of the lipase determined by sds-page was 97 kda. purified a. sobria lp004 lipase exhibited the maximum activity at ph 6.0 and 45 degrees c and was stable under alkaline conditions (ph 6.5-10.0) and at temperatures lower than 40 degrees c. this lipase could be cla ...19979195755
antidiarrhoeal activities of ocimum gratissimum (lamiaceae).the antidiarrhoeal activities of leaf extracts of ocimum gratissimum were investigated by disc diffusion and tube dilution methods. the extracts were active against aeromonas sobria, escherichia coli, plesiomonas shigelloides, salmonella typhi, and shigella dysenteriae. the leaf extracts were most active against s. dysenteriae and least active against s. typhi. the sensitivity of the organisms measured in terms of zone of inhibition ranged from 8.00 to 19.50 mm. the minimum inhibitory concentrat ...19969203793
[bacteremia for aeromonas sobria in a patient with cholecystitis]. 19979221025
evaluation of the synergistic haemolysis (camp-like) test in the identification of motile, mesophilic aeromonas species.usefulness of the synergistic haemolysis (camp-like) test in the identification of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae was evaluated, using beta-toxin producing staphylococcus aureus atcc 25923 and alpha-toxin producing clostridium perfringens atcc 13124 as sphingomyelinase producing indicator strains. the tests were done on columbia blood agar base supplemented with 5% sheep blood (cba; difco laboratories, detroit, mi, usa). when cultured aerobically, 77% of a. hydrophi ...19979241799
bacterial diarrhea in laos, a region where cholera was endemic.there were cholera outbreaks in lao pdr since 1993. two provinces were epidemic areas of cholera in 1993. however the area of cholera occurrence increased to 7 provinces through 1994. then a bacterial survey had done for the purpose of public health improvement. epec was markedly isolated from the hospital in vientiane. in apparent infection of salmonella and non-01 vibrio cholerae with soldiers stationed in the island in mekong river belong to vientiane were pointed out. the outbreak of diarrhe ...19969253874
aeromonas sobria-associated left-sided segmental colitis.aeromonas species, once thought pathogenic only in immunocompromised hosts, have more recently been found to be associated with many different infections in previously healthy individuals. the most common site of these infections is the gastrointestinal tract. we describe a 67-yr-old woman who presented with abdominal pain and bloody diarrhea and was diagnosed with left-sided, segmental colitis due to aeromonas sobria, which was proven by stool culture. extensive work-up for ischemic colitis was ...19979362202
aeromonas sobria endophthalmitis.aeromonas sobria causes a rare gram-negative bacterial water-borne infection. it has been found in waters of north queensland and south-east asia. of all aeromonas species, a. sobria is the most virulent and invasive and has been reported to cause soft tissue infection and corneal ulcer.19979395834
virulence factors in aeromonas spp and their association with gastrointestinal disease.culture filtrates of eight aeromonas strains isolated from the feces of 487 subjects (292 diarrhoeic patients and 195 asymptomatic subjects) were tested for toxin production in cho and mccoy cells, and adhesion and invasive ability in caco-2 cells. among these isolates, three aeromonas sobria and one aeromonas caviae strains possessed virulence-associated properties. toxin production was the most common of the three virulence properties. two a. caviae were associated, in the absence of other dia ...19989497926
acute rhabdomyolysis and myonecrosis complicating aeromonas bacteremia in neutropenic patients with hematologic malignancies: report of two cases.infections by aeromonas spp. are a rare cause of systemic infection in normal and immunocompromised hosts. we report the cases of two patients with acute non-lymphoblastic leukemia who developed septic shock by aeromonas species with unusual soft-tissue complications. one patient who was undergoing consolidation chemotherapy developed septic shock by aeromonas hydrophila with rhabdomyolysis and subsequent soft-tissue destruction consistent with myonecrosis. she recovered with combination antibio ...19979499669
molecular cloning, nucleotide sequence, and expression in escherichia coli of a hemolytic toxin (aerolysin) gene from aeromonas trota.aeromonas trota ak2, which was derived from atcc 49659 and produces the extracellular pore-forming hemolytic toxin aerolysin, was mutagenized with the transposon mini-tn5km1 to generate a hemolysin-deficient mutant, designated strain ak253. southern blotting data indicated that an 8.7-kb noti fragment of the genomic dna of strain ak253 contained the kanamycin resistance gene of mini-tn5km1. the 8.7-kb noti dna fragment was cloned into the vector pgem5zf(-) by selecting for kanamycin resistance, ...19989647817
occurrence, diversity and pathogenicity of mesophilic aeromonas in estuarine waters of the italian coast of the adriatic sea.a total of 208 strains of aeromonas were isolated by monthly sampling from two estuaries (one provided with, and the other devoid of a waste-water treatment system) on the italian coast of the adriatic sea between september 1994 and august 1995. biotyping at the species level allowed the identification of 96 strains (46%) as aer. caviae, 46 (22%) as aer. sobria, 33 (16%) as aer. hydrophila and 25 (12%) as aer. veronii. eight strains (4%) were regarded as unnamed aeromonads. aeromonas caviae was ...19989750281
[camp factor for the differentiation among aeromonas species].the camp factor technique, described by natale figura as a presumptive method for the differentiation of the species hydrophila, sobria and caviae, was applied to 80 aeromonas strains isolated from children under 5 years with acute diarrheic disease. the typical phenomenon was seen in aerobiosis and anaerobiosis conditions in 10 strains, which were classified as aeromonas hydrophila. in aerobiosis conditions/alone, it was observed in 20 strains, which were identified as aeromonas sobria. it was ...19929768204
the acute phase response in the sudan plated lizard, gerrhosaurus major.the sudan plated lizard (gerrhosaurus major), previously reported to be an afebrile species, was utilized in a series of experiments to test for various aspects of the acute phase response. treatment of individuals with the antibiotic baytril resulted in a slight (0.5 degree c) but significant reduction in mean selected body temperature (msbt), while treatment with saline did not lower msbt. nonantibiotic treatment individuals had depressed plasma iron levels (86.6 +/- 22.4 micrograms fe 100 ml- ...19989773512
[phenotype markers in strains of aeromonas isolated in cuba from children with an acute diarrheic disease].twenty-seven cases of aeromonas isolated from 300 five-years children with acute diarrheal disease (eda) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. the percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher th ...19959805080
purification and characterization of enterotoxin produced by aeromonas sobria.we purified the toxin of aeromonas sobria capable of inducing a positive response in the mouse intestinal loop assay. the purified toxin showed a positive response not only in the loop assay but also in a hemolytic assay. subsequently, we cloned the toxin gene and demonstrated that the product of this gene possessed both hemolytic and enterotoxic activities. these results showed that the enterotoxin of a. sobria possesses hemolytic activity. nucleotide sequence determination of the toxin gene an ...19989858466
[meningitis by aeromonas sobria following cranio-encephalic traumatism]. 19989919002
secretion of hemolysin of aeromonas sobria as protoxin and contribution of the propeptide region removed from the protoxin to the proteolytic stability of the toxin.the sequence at the amino terminus region of the hemolysin ofaeromonas sobria is homologous with that of aerolysin of a. hydrophila. however, there is no homology between the two toxins in the sequence at the carboxy terminal region. it has been shown that aerolysin is secreted into culture supernatant as a protoxin. this proaerolysin is activated by the proteolytic removal of a carboxy terminal peptide. however, the role of the carboxy terminal region, which is removed in the activation process ...199910100744
unusual case of aeromonas sobria cellulitis associated with the use of leeches. 199910192721
[bacteriological studies of traveller's diarrhoea (6). analysis of enteropathogenic bacteria at kansai airport quarantine station from september 4th, 1994 through december 1996].during the period of investigation from sept. 4, 1994 to dec, 1996, a total of 11,446,534 overseas travellers were quarantined at kansai airport quarantine station, and 22,187 voluntarily reported of episodes suffering from diarrhoea. bacteriological examination of the stools a total of 9,299 individuals' was performed, and the following results were obtained. 1) various enteropathogenic bacteria were isolated from 33.3% of the stools examined. bacterial species isolated were as follows: plesiom ...199910213987
[rapidly fatal prognosis caused by aeromonas sobria septic shock in a patient with myelodysplastic syndrome]. 199910222675
important aspects of the colonization of central venous catheter.this study comprises five different kind of venous central catheters, 103 in total, made of polyurethane tecoflex, polyurethane vialon, ptfe and pvc, and the influence of their raw material on the microbial colonization. patients age and sex, besides their clinical conditions, were taken into account, and neither considered as a sample vicious, nor associated with colonization. when the tips of the catheters were asseptically inoculated in tryptic soy broth and tioglicolate, colonization was det ...199910326311
enumeration and confirmation of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria isolated from raw milk and other milk products in northern greece.a total of 138 raw cow's and 57 raw ewe's milk samples; 80 pasteurized cow's milk samples; 39 anthotyros cheese, 36 manouri cheese, and 23 feta cheese samples; and 15 rice pudding samples were examined for the presence and any countable population of aeromonas species. twenty-two (15.9%) of the 138 cow's milk samples analyzed were contaminated with a. hydrophila. in 13 of these samples, populations of 3.0x10(2) to 5.0x10(3) cfu/ml were counted in starch ampicillin agar (saa). eighteen cow's milk ...199910340665
hemolysin of aeromonas sobria stimulates production of cyclic amp by cultured cells.hemolysin of aeromonas sobria possesses both cytotoxic activity against mammalian cells and enterotoxic activity. histopathological examination revealed that hemolysin causes diarrhea without damaging the intestinal epithelial cells. and the fluid accumulated in the mouse intestinal loop by the action of the hemolysin is watery. these observations indicated that the enterotoxic activity of hemolysin is not dependent on its cytotoxic activity. to clarify the mechanism of the enterotoxic action of ...199910418132
aeromonas sobria infection with severe soft tissue damage and segmental necrotizing gastroenteritis in a patient with alcoholic liver cirrhosis.a 49-year-old man, who had a 3-year history of liver dysfunction but had not been treated, was admitted to the hospital with a sudden onset of fever and generalized muscle pain. he subsequently developed generalized purpura with scattered hemorrhagic bullae of the skin and massive bloody stools. aeromonas sobria was proven by culture of both blood and bullous fluid. in spite of the extensive treatment with antibiotics and other medications in the intensive care unit (icu), the patient went into ...199910469397
identification and characterization of the aeromonas sobria hemolysin glycoprotein receptor on intestine 407 cells.aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. by immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kda protein (p66) was identified as a receptor for a. sobria hemolysin on intestine 407 cells. treatment of p66 with n-glycosidase f reduced the apparent sized of p66 to 60 kda on sds-polyacrylamide gels. p66, released from intestine 407 cells following incubation with phosphatidylinositol-specific ph ...199910502462
occurrence of haemolytic & cytotoxic aeromonas species in domestic water supplies in chennai.a study on the occurrence of aeromonas species in the domestic water supplies in chennai showed that as much as 37.9 per cent of the water samples analyzed from various sources harbored aeromonas spp. majority of the isolates belonged to aeromonas sobria (13.7%), a. caviae (11.6%) and a. hydrophila (9.5%). among the 37 metropolitan water samples analyzed, 11 samples yielded aeromonas spp. inclusive of three isolates of a. hydrophila, four of a. sobria and two isolates each of a. caviae and a. ja ...199910573655
carboxy terminal region of haemolysin of aeromonas sobria triggers dimerization.haemolysin of aeromonas sobria is released into the culture supernatant in the form of prohaemolysin. removal of a 42 amino acid peptide at the carboxy-terminal end converts prohaemolysin into mature haemolysin. as the role of the peptide removed from the mature haemolysin has not been studied, we mutated the haemolysin genes to delete several amino acid residues from the carboxy terminus, expressed the mutant genes in a. sobria and analysed the haemolysins produced. deletion of more than three ...200010623561
acute renal failure in an infant associated with cytotoxic aeromonas sobria isolated from patient's stool and from aquarium water as suspected source of infection. 200010681210
combined infection of norwalk-like virus and verotoxin-producing bacteria associated with a gastroenteritis outbreak.detection of multiple pathogens, particularly a combination of viruses and bacteria, is infrequently documented in outbreaks of gastroenteritis. this paper reports the presence of norwalk-like virus (nlv) and enterohaemorrhagic verotoxin-producing escherichia coli in one individual, and nlv and verotoxin-producing aeromonas sobria in another individual, both part of a large gastroenteritis outbreak. the causes of gastroenteritis in such outbreaks may be more complex than previously thought.199910892495
antimicrobial resistance of mesophilic aeromonas spp. isolated from two european rivers.the activity of 19 antibiotics and four antiseptics and/or disinfectants was studied against 138 non-redundant strains of aeromonas spp. (104 aeromonas caviae, 22 aeromonas sobria and 12 aeromonas hydrophila) isolated from two european rivers. antibiotic resistance frequencies were: nalidixic acid, 59%; tetracycline, 14%; fosfomycin, 8%; tobramycin and cotrimoxazole, 7%; cefotaxime, 4%; chloramphenicol, 2%; gentamicin, 1%. most of the nalidixic acid-resistant strains were susceptible to fluoroqu ...200010933657
inhibition of aeromonas caviae and a. sobria by sodium choloride, citric acid, ascorbic acid, potassium sorbate and extracts of thymus vulgaris.the respective and combined effects of sodium chloride, ascorbic acid, citric acid, potassium sorbate, and thymus vulgaris extract on the growth of aeromonas caviae and aeromonas sobria were investigated. sodium chloride (3%) significantly reduced the growth and 4% nacl inhibited growth of the tested strains. ascorbic acid (0. 1%), potassium sorbate (0.05%), and citric acid (0.03%) slightly inhibited growth. t. vulgaris extract (0.3%) greatly reduced the growth. various combinations of these com ...200010957708
[cytoplasmic protein vaccine against bacterial hemorrhagic septicemia (aeromonosis) of fish].water-soluble proteins from aeromonas sobria, a causative agent of bacterial hemorrhagic septicemia of fishes, were separated into six fractions by gel chromatography on sephadex g-100. injections of fraction ii (67 kda) provided the highest protection of carps against the disease. injections of proteins contained in fraction ii caused stronger effects on certain biochemical parameters in the fish liver (fatty acids of phospholipids and cathepsin b and d activities) in comparison to infections o ...200011042886
effect of sodium chloride and citric acid on growth and toxin production by a. caviae and a. sobria at moderate and low temperatures.the effect of sodium chloride and citric acid on hemolysin and caseinase production by aeromonas caviae and aeromonas sobria at 32 degrees c and 5 degrees c was investigated. at 32 degrees c, although both strains were tolerant to 3% nacl in tsb, the production of caseinase was decreased in the presence of 1-3% nacl, and the production of hemolysin was abolished by 2-3% nacl. citric acid (0.03%) was less effective than nacl in reducing hemolysin and caseinase production by both strains at 32 deg ...200011061632
production of serine protease of aeromonas sobria is controlled by the protein encoded by the gene lying adjacent to the 3' end of the protease gene.we cloned a protease gene of aeromonas sobria and determined its nucleotide sequence. the protease is composed of 624 amino acid residues and its calculated molecular weight is 66,737.7. the amino acid sequence showed the characteristic features of a bacterial serine protease. we expressed the protease gene in vibrio parahaemolyticus from which the synthesized protease is secreted into the culture medium as the mature form, and purified the mature protease by successive column chromatographies. ...200011092244
[microbiological monitoring of causative agents of sapronoses in the water of the bogatinskoe water reservoir].the results of the microbiological monitoring of potential causative agents of sapronoses in the water of the bogatinskoye reservoir revealed that in the summer period of 1998 the mass accumulation of virulent aeromonas sobria (up to 25% of the total number of heterotrophic bacteria) took place. the autumn period was characterized by a decrease in the number of a. sobria and the detection of bacteria of the genus vibrio (up to 22%) with v. mimicus and v. metschnikovii identified among them in th ...200011220958
aeromonas sobria sepsis in a neutropenic patient. 200111325663
characterization of aeromonas and vibrio species isolated from a drinking water reservoir.to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species.200111412322
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