Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| characterization of susceptibility and carrier status of burbot, lota lota (l.), to ihnv, ipnv, flavobacterium psychrophilum, aeromonas salmonicida and renibacterium salmoninarum. | in this study, susceptibility and potential carrier status of burbot, lota lota, were assessed for five important fish pathogens. burbot demonstrated susceptibility and elevated mortality following challenge with infectious haematopoietic necrosis virus (ihnv) by immersion and to aeromonas salmonicida by intraperitoneal (i.p.) injection. ihnv persisted in fish for at least 28 days, whereas a. salmonicida was not re-isolated beyond 17 days post-challenge. in contrast, burbot appeared refractory t ... | 2010 | 20367740 |
| unmethylated cpg oligodeoxynucleotides activate head kidney leukocytes of atlantic cod, gadus morhua. | bacterial dna and synthetic oligodeoxynucleotides (odns) that contain unmethylated cpg motifs are strong inducers of immune response in most mammalian organisms. the use of these synthetic cpg motifs in fish, particularly in salmonids and carp, resulted in the modulation of their immune system. however, much less is known in other species of fish such as gadoids including atlantic cod, gadus morhua. using head kidney (hk) leukocytes of cod in an in vitro study, we determined the effects of some ... | 2010 | 20349339 |
| first description of atypical furunculosis in freshwater farmed atlantic salmon, salmo salar l., in chile. | we report the first isolation, identification and characterization of a group of chilean strains of atypical aeromonas salmonicida isolated from freshwater farmed atlantic salmon, salmo salar. affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. outbreaks of the disease occurred in two farms in the south of chile about 2200 km apart. five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging ... | 2010 | 20298453 |
| thiaminase activity of crucian carp carassius carassius injected with a bacterial fish pathogen, aeromonas salmonicida subsp. salmonicida. | dietary thiaminase i is a cause of thiamine deficiency in animals. the physiological significance of thiaminase in the organisms containing this enzyme is not known, nor are the factors causing variation in their thiaminase activity. tests were performed to evaluate the effect a pathogen might have on thiaminase activity in fish, when analyzed both with a cosubstrate added (cata tests) and no cosubstrate added (ncata tests). pyridine is known as a cosubstrate specific for thiaminase i activity t ... | 2009 | 20218496 |
| in vivo transfer of plasmid pras1 between aeromonas salmonicida and aeromonas hydrophila in artificially infected cyprinus carpio l. | this study investigated the possible in vivo transfer of plasmid pras1 between aeromonas salmonicida and a. hydrophila inhabiting two different organs of cyprinus carpio l. to distinguish transconjugants from naturally occurring antibiotic resistant bacteria, twelve luminescent transposon-tagged a. hydrophila strains using mini tn5luxcdabekm2 transposon were generated. in conjugal transfer experiments, fish were conditioned with the donor bacteria and subsequently immersed in water containing th ... | 2010 | 20059637 |
| concurrent injection of a rhabdovirus-specific dna vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in atlantic salmon, salmo salar l. | vaccines are commonly used in salmonid aquaculture as a method of disease prevention. although there is a substantial amount of published research regarding the immunological and physiological effects following the injection of different polyvalent vaccines and dna vaccines, there are no published reports examining the physiological and immunological effects of concurrent vaccine injection, which is the situation encountered in aquaculture. using key immunological parameters such as lysozyme act ... | 2010 | 20045062 |
| identification of a novel allele of peroxisome proliferator-activated receptor gamma (pparg) and its association with resistance to aeromonas salmonicida in atlantic salmon (salmo salar). | bacterial and viral diseases are major problems in atlantic salmon aquaculture, but may be challenged through selection of brood stock with enhanced survival to diseases. today's selection strategy is based on controlled challenge tests using siblings of the breeding candidates, and is thus indirect. direct trait records on breeding candidates can potentially be provided through identification of genetic variation linked to the susceptibility to the disease. peroxisome proliferator-activated rec ... | 2010 | 20004720 |
| the reference strain aeromonas hydrophicla cip 57.50 should be reclassified as aeromonas salmonicida cip 57.50. | the use of reference strains is a critical element for the quality control of different assays, from the development of molecular methods to the evaluation of antimicrobial activities. most of the strains used in these assays are not type strains and some of them are cited erroneously because of subsequent reclassifications and descriptions of novel species. in this study, we propose that the reference strain aeromonas hydrophila cip 57.50 be reclassified as aeromonas salmonicida cip 57.50 based ... | 2010 | 19965992 |
| glycan flexibility: insights into nanosecond dynamics from a microsecond molecular dynamics simulation explaining an unusual nuclear overhauser effect. | an atomistic all-atom molecular dynamics simulation of the trisaccharide beta-d-manpnac-(1-->4)[alpha-d-glcp-(1-->3)]-alpha-l-rhap-ome with explicit solvent molecules has been carried out. the trisaccharide represents a model for the branching region of the o-chain polysaccharide of a strain from aeromonas salmonicida. the extensive md simulations having a 1-micros duration revealed a conformational dynamics process on the nanosecond time scale, that is, a 'time window' not extensively investiga ... | 2010 | 19962132 |
| multiplex pcr method for detection of three aeromonas enterotoxin genes. | a novel multiplex pcr method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in aeromonas spp. this assay was used to characterize 35 reference strains as well as 537 food-borne isolates. a total of seven gene pattern combinations were encountered, including act, alt, act/alt, act/alt/ast, act/alt/148-bp amplicon, alt/ast, and alt/148-bp amplicon. the alt gene was detected with 34 reference st ... | 2010 | 19933350 |
| characterization and expression analyses of anti-apoptotic bcl-2-like genes nr-13, mcl-1, bcl-x1, and bcl-x2 in atlantic cod (gadus morhua). | nr-13, mcl-1, and bcl-x(l), are conserved anti-apoptotic proteins that belong to the anti-apoptotic bcl-2 sub-family, which inhibits cell death by preventing mitochondrial membrane permeabilization (mmp). given the anti-apoptotic functions of these proteins in vertebrates (e.g. human, mouse, and zebrafish) and the involvement of apoptotic regulation in immune responses, we studied the sequences of these genes and their transcript expression in atlantic cod (gadus morhua) during innate immune res ... | 2010 | 19923001 |
| mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels. | mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ... | 2010 | 19900538 |
| outbreaks of an ulcerative and haemorrhagic disease in arctic char salvelinus alpinus caused by aeromonas salmonicida subsp. smithia. | arctic char salvelinus alpinus farmed in different places in austria and free of the viral diseases viral haemorrhagic septcaemia (vhs), infectious haematopoietic necrosis (ihn) and infectious pancreatic necrosis (ipn) experienced disease and mortality. diseased fish showed skin ulceration and pathological signs of sepsis. aeromonas sp. was isolated as pure culture from the kidney of freshly euthanized diseased fish. three independent isolates from outbreaks that occurred on 2 of the affected fa ... | 2009 | 19899353 |
| genetic insertions and diversification of the polb-type dna polymerase (gp43) of t4-related phages. | in escherichia coli phage t4 and many of its phylogenetic relatives, gene 43 consists of a single cistron that encodes a polb family (polb-type) dna polymerase. we describe the divergence of this phage gene and its protein product (gp43) (gene product 43) among 26 phylogenetic relatives of t4 and discuss our observations in the context of diversity among the widely distributed polb enzymes in nature. in two t4 relatives that grow in aeromonas salmonicida phages 44rr and 25, gene 43 is fragmented ... | 2010 | 19896487 |
| hepatic gene expression profiling reveals protective responses in atlantic salmon vaccinated against furunculosis. | furunculosis, a disease caused with gram negative bacteria aeromonas salmonicida produces heavy losses in aquaculture. vaccination against furunculosis reduces mortality of atlantic salmon but fails to eradicate infection. factors that determine high individual variation of vaccination efficiency remain unknown. we used gene expression analyses to search for the correlates of vaccine protection against furunculosis in atlantic salmon. | 2009 | 19878563 |
| are all koi ulcer cases associated with infection by atypical aeromonas salmonicida? polymerase chain reaction assays of koi carp skin swabs submitted by hobbyists. | infection by atypical aeromonas salmonicida is regarded as the cause of ulcer disease (kud) in koi carp cyprinus carpio and goldfish carassius auratus. however, other causes--including parasites, viral infection, and fungi--have been proposed. in our diagnostic work, we often fail to isolate a. salmonicida even when clear clinical signs of kud are present. this failure may be because these fastidious and slow-growing bacteria are difficult to isolate in culture or because the bacteria are not ac ... | 2009 | 19873831 |
| responses of atlantic cod gadus morhua head kidney leukocytes to phytase produced by gastrointestinal-derived bacteria. | this study identified phytase-producing bacteria that were previously isolated from the gastrointestinal tract of atlantic cod, gadus morhua and determined its effect on head kidney leukocytes. out of the 216 bacterial strains tested, the two phytase producers were identified as pseudomonas sp. and psychrobacter sp. based on their 16s rdna sequence. crude phytase from these two bacterial strains was produced employing the shake flask method. even though the total protein of the crude phytase was ... | 2010 | 19844802 |
| design and performance of a turbot (scophthalmus maximus) oligo-microarray based on ests from immune tissues. | an expressed sequence tag database from immune tissues was used to design the first high-density turbot (scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. specific oligonucleotides (60 mers) were successfully designed for 2,716 out of 3,482 unique sequences of the database. an agilent custom oligo-microarray 8 x 15 k (five replicates/gene; eight microarrays/slide) was constructed. the performance of the microarray and the sources of va ... | 2010 | 19844759 |
| a bifunctional enzyme in a single gene catalyzes the incorporation of glcn into the aeromonas core lipopolysaccharide. | the core lipopolysaccharide (lps) of aeromonas hydrophila ah-3 and aeromonas salmonicida a450 is characterized by the presence of the pentasaccharide alpha-d-glcn-(1-->7)-l-alpha-d-hep-(1-->2)-l-alpha-d-hep-(1-->3)-l-alpha-d-hep-(1-->5)-alpha-kdo. previously it has been suggested that the waha protein is involved in the incorporation of glcn residue to outer core lps. the waha protein contains two domains: a glycosyltransferase and a carbohydrate esterase. in this work we demonstrate that the in ... | 2009 | 19805547 |
| mutations in the aeromonas salmonicida subsp. salmonicida type iii secretion system affect atlantic salmon leucocyte activation and downstream immune responses. | deletion mutants of aeromonas salmonicida subsp. salmonicida were used to determine the effect of the type three secretion system (ttss) on atlantic salmon anterior head kidney leucocytes (ahkl). one strain had a deletion in the outer membrane pore gene, ascc; and the other in three effector genes: aopo, aoph and aext (we call this strain deltaaop3). host cell invasion success and 24h survival were depressed in deltaascc, as was 24h survival of deltaaop3, when compared to the wild type strain. c ... | 2009 | 19751835 |
| interleukin-17d in atlantic salmon (salmo salar): molecular characterization, 3d modelling and promoter analysis. | il-17 is a proinflammatory cytokine that plays an important role in the clearance of extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. much work on il-17 has been done in humans and higher vertebrates while little work has been conducted in lower vertebrates including fish. in this study, we have cloned and characterized the full-length cdna and genomic sequence of il-17d from atlantic salmon. the atlantic salmon il-17d (asil-17d) cdna possessed ... | 2009 | 19716422 |
| atypical furunculosis vaccines for atlantic cod gadhus morhua: impact of reattached aeromonas salmonicida a-layer protein on vaccine efficacy. | atypical furunculosis caused by atypical aeromonas salmonicida bacteria is reported as an increasing problem in farmed atlantic cod gadus morhua in norway. at present, furunculosis vaccines adapted for cod or other marine fish species are not available. to identify bacterial components important for inducing protection in cod, we compared oil-adjuvanted vaccines based on a. salmonicida isolates phenotypically differing in their major cell surface constituents, such as the a-layer protein and lip ... | 2009 | 19694171 |
| tri-trophic linkages in disease: pathogen transmission to rainbow trout through stonefly prey. | relationships between macroinvertebrates and microorganisms in aquatic environments are only poorly understood despite the fact that many aquatic macroinvertebrates feed on microbial biofilms during some life stage. better understanding of trophic interactions between microbial biofilms, macroinvertebrates, and fish may also help control fish diseases and loss of natural resources. it has also been suggested that pollution, habitat fragmentation, and poor water quality may contribute to increase ... | 2009 | 19689880 |
| in vivo modulation of immune response and antioxidant defense in atlantic cod, gadus morhua following oral administration of oxolinic acid and florfenicol. | oxolinic acid and florfenicol are the commonly used antibiotics for the treatment of bacterial diseases in atlantic cod, gadus morhua. the changes in selected innate humoral immune response of the fish, bacterial proliferation in serum and transcriptional activity of selected immune and antioxidant defense-related genes following oral administration of these antimicrobial compounds were evaluated. juvenile cod (75-100 g) were fed commercial feed coated with either florfenicol (10 mg kg(-1) fish, ... | 2009 | 19596082 |
| transcription factor gata-3 in atlantic salmon (salmo salar): molecular characterization, promoter activity and expression analysis. | gata-3 is a t cell-specific transcription factor and is essential for the development of the t cell lineage and differentiation of t helper type 2 cells. we have identified and characterized the full-length atlantic salmon gata-3 cdna (3074bp), having two zinc finger domains which are fully conserved within teleosts and higher vertebrates. rt-pcr analysis revealed that the atlantic salmon gata-3 (asgata-3) is strongly expressed in gills, thymus, and brain. moreover, the involvement of gata-3 in ... | 2009 | 19576635 |
| crystallization and preliminary x-ray diffraction studies of asap1_e294a and asap1_e294q, two inactive mutants of the toxic zinc metallopeptidase asap1 from aeromonas salmonicida subsp. achromogenes. | two mutants of the toxic extracellular zinc endopeptidase asap1 (asap1_e294q and asap1_e294a) of aeromonas salmonicida subsp. achromogenes were expressed in escherichia coli and crystallized by the vapour-diffusion method. crystals were obtained using several precipitants and different protein concentrations. protein crystals were found in a monoclinic (c2) as well as an orthorhombic (p2(1)2(1)2(1)) space group. the crystals belonging to the monoclinic space group c2 had unit-cell parameters a = ... | 2009 | 19574642 |
| aeromonas piscicola sp. nov., isolated from diseased fish. | four aeromonas strains (s1.2(t), eo-0505, tc1 and ti 1.1) isolated from moribund fish in spain showed a restriction fragment length polymorphism (rflp) pattern related to strains of aeromonas salmonicida and aeromonas bestiarum but their specific taxonomic position was unclear. multilocus sequence analysis (mlsa) of housekeeping genes rpod, gyrb, reca and dnaj confirmed the allocation of these isolates to an unknown genetic lineage within the genus aeromonas with a. salmonicida, a. bestiarum and ... | 2009 | 19570633 |
| effect of lactococcus lactis clfp 100 and leuconostoc mesenteroides clfp 196 on aeromonas salmonicida infection in brown trout (salmo trutta). | aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. this pathogen is important from an epizootic perspective because fish surviving an outbreak can remain lifelong asymptomatic carriers, serving as reservoirs of infection. as a result, the early detection and the control of infection are essential to prevent the spread of new furunculosis outbreaks. we have thus analyzed the effect of probiotic administration on the incidence of a. salmonicida in brown trout (salmo t ... | 2009 | 19556745 |
| aeromonas salmonicida subsp. salmonicida type strain does not possess a type iii secretion system. | 2009 | 19553573 | |
| characterization of probiotic carnobacteria isolated from rainbow trout (oncorhynchus mykiss) intestine. | to characterize two probiotic carnobacterial isolates, carnobacterium maltaromaticum (b26) and c. divergens (b33), derived from rainbow trout (oncorhynchus mykiss) intestine. | 2008 | 19552776 |
| plasma ladderlectin concentration following sterile inflammation and aeromonas salmonicida subsp. salmonicida infection. | plasma samples obtained from rainbow trout either experimentally infected with aeromonas salmonicida or injected with either a. salmonicida lipopolysaccharide (lps) or a commercial a. salmonicida vaccine (lipogen) were analysed by enzyme immunoassay to evaluate changes in rainbow trout ladderlectin (rtll) concentrations during the acute phase response (apr). plasma rtll concentrations in fish injected with a. salmonicida lps, vaccine or live a. salmonicida varied over a 10 day period, but did no ... | 2009 | 19538251 |
| a novel packaging method with a dissolving co(2) headspace combined with organic acids prolongs the shelf life of fresh salmon. | the aim of this study was to evaluate a novel packaging method for fresh fish and to determine its effect on the bacterial growth in fresh salmon. fresh salmon was packed with a small amount of 100% co(2) (gas/product ratio 0.2/1.0 v/v) and a brine solution containing various combinations of citric acid (3% w/w, ph 5), acetic acid (1% w/w, ph 5) and cinnamaldehyde (200 microg/ml). total bacterial counts, counts of sulphur reducing bacteria, lactic acid bacteria and enterobacteriaceae as well as ... | 2009 | 19523706 |
| the effect of hyperoxygenation and reduced flow in fresh water and subsequent infectious pancreatic necrosis virus challenge in sea water, on the intestinal barrier integrity in atlantic salmon, salmo salar l. | in high intensive fish production systems, hyperoxygenation and reduced flow are often used to save water and increase the holding capacity. this commonly used husbandry practice has been shown to be stressful to fish and increase mortality after infectious pancreatic necrosis virus (ipnv) challenge, but the cause and effect relationship is not known. salmonids are particularly sensitive to stress during smoltification and the first weeks after seawater (sw) transfer. this work aimed at investig ... | 2009 | 19500205 |
| application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the o-chain polysaccharide of aeromonas salmonicida from in vitro- and in vivo-grown cells. | in this study, application of magnetic beads (dynabeads) coated with aeromonas salmonicida lipopolysaccharide-specific polyclonal antisera to ms-based characterization of bacterial lipopolysaccharides has been evaluated. the results showed that the affinity-based preconcentration strategy resulted in at least a 100-fold increase in the detection of sensitivity, affording direct capillary electrophoresis (ce)-ms analysis of a. salmonicida lipopolysaccharide o-chain polysaccharide from in vitro-cu ... | 2009 | 19456871 |
| differences in the antibody response and survival of genetically different varieties of common carp (cyprinus carpio l.) vaccinated with a commercial aeromonas salmonicida/a. hydrophila vaccine and challenged with a. hydrophila. | males of two strains of carp, wild duna (d), and inbred szarvas 22 (22), were selected for high and low stress response. two purebreds of d and 22, from randomly chosen parents and four crosses, 22 x 22-l (low stress response), 22 x 22-h (high stress response), 22 x d-l (low stress response) and 22 x d-h (high stress response) from selected stress response parents were produced and vaccinated with a commercial aeromonas salmonicida/aeromonas hydrophila vaccine and their circulating antibody resp ... | 2009 | 19449165 |
| isolation and cdna cloning of an antibacterial l-amino acid oxidase from the skin mucus of the great sculpin myoxocephalus polyacanthocephalus. | the skin mucus of the great sculpin myoxocephalus polyacanthocephalus showed both antibacterial and l-amino acid oxidase (lao) activities. antibacterial laos were purified from the skin mucus of the m. polyacanthocephalus by column chromatography and named mplao1, mplao2, and mplao3, based on the order of elution by ion-exchange high performance liquid chromatography. cdna cloning of mplao3 revealed that the full-length cdnawas 2659 bp and encoded the signal peptide (met1-ala26) and the mature p ... | 2009 | 19447190 |
| quantitative analysis of bacterial adhesion to fish tissue. | adhesion to host tissue represents a first crucial step in most bacterial infections. both specific adhesion-ligand as well as hydrophobic interactions may be involved. the adhesion of aeromonas salmonicida subsp. salmonicida, lactococcus garvieae, and yersinia ruckeri strains to fish tissue cells was assessed. to determine whether the observed bacterial adhesion to fish tissue cells was caused by non-specific interactions, adhesion to bovine serum albumin (bsa) and polystyrene was also tested. ... | 2009 | 19349152 |
| myxinidin, a novel antimicrobial peptide from the epidermal mucus of hagfish, myxine glutinosa l. | fish epidermal mucus contains innate immune components that provide a first line of defense against various infectious pathogens. this study reports the bioassay-guided fractionation and characterization of a novel antimicrobial peptide, myxinidin, from the acidic epidermal mucus extract of hagfish (myxine glutinosa l.). edman sequencing and mass spectrometry revealed that myxinidin consists of 12 amino acids and has a molecular mass of 1,327.68 da. myxinidin showed activity against a broad rang ... | 2009 | 19330556 |
| purification, virulence and characterization of an extracellular peptidase produced by aeromonas salmonicida ssp. masoucida isolated from cultured sea cucumber apostichopus japonicus. | strain h1, aeromonas salmonicida ssp. masoucida isolated from an ulcer on the sea cucumber apostichopus japonicus proved to be virulent toward this holothurian at 12 degrees c. extracellular products (ecp) of strain h1 were prepared, and an extracellular peptidase of 42 kda was purified from the ecp using sephadex g-100 gel filtration chromatography and diethylaminoethyl sepharose ion exchange chromatography. stepwise virulence tests on sea cucumbers indicated that the 42 kda peptidase was the v ... | 2008 | 19244974 |
| identification and analysis of differentially expressed genes in immune tissues of atlantic cod stimulated with formalin-killed, atypical aeromonas salmonicida. | physiological changes, elicited in animal immune tissues by exposure to pathogens, may be studied using functional genomics approaches. we created and characterized reciprocal suppression subtractive hybridization (ssh) cdna libraries to identify differentially expressed genes in spleen and head kidney tissues of atlantic cod (gadus morhua) challenged with intraperitoneal injections of formalin-killed, atypical aeromonas salmonicida. of 4,154 ests from four cdna libraries, 10 genes with immune-r ... | 2009 | 19240301 |
| [taxonomic characteristics and physiological properties of microorganisms from the gut of pike (esox lucius)]. | the taxonomic composition and distribution of microorganisms differing in the degree of association with the intestinal mucosa of the pike (lucius lucius) has been studied. microorgansism of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae dominate in the gut microflora. numerically prevailing bacterial species are characterized by high proteolytic and amylolytic enzyme activities as well as by high persistence accounted for by antilysozyme and antihistone activities. the result ... | 2008 | 19198074 |
| genetic characterization of pasa6, a new plasmid from aeromonas salmonicida subsp. salmonicida that encodes a type iii effector protein aoph homolog. | a new plasmid designated pasa6 from an aeromonas salmonicida subsp. salmonicida strain isolated from diseased turbot has been characterized. pasa6 consists of 18536bp, has a g+c content of 53.8% and encodes 20 predicted open-reading frames (orfs). eight orfs showed homology to transposases, of which six are complete and two are partial is sequences. two orfs showed homology to replication proteins, and six orfs showed homology to hypothetical proteins. two orfs are truncated homologs of putative ... | 2009 | 19195484 |
| genetics and proteomics of aeromonas salmonicida lipopolysaccharide core biosynthesis. | comparison between the lipopolysaccharide (lps) core structures of aeromonas salmonicida subsp. salmonicida a450 and aeromonas hydrophila ah-3 shows great similarity in the inner lps core and part of the outer lps core but some differences in the distal part of the outer lps core (residues ld-hep, d-gal, and d-galnac). the three genomic regions encoding lps core biosynthetic genes in a. salmonicida a450, of which regions 2 and 3 have genes identical to those of a. hydrophila ah-3, were fully seq ... | 2009 | 19151135 |
| characterization and comparative analyses of zebrafish intelectins: highly conserved sequences, diversified structures and functions. | intelectin family, also called the x-lectin family, is a newly discovered gene family involved in development and innate immunity. however, no research was carried out for this gene family in the model organism zebrafish. here we present the first characterization of seven zebrafish intelectins (zintls) and the first systematic comparative analysis of intelectins from various species in order to provide some clues to the function and evolution of this gene family. we examined the expression patt ... | 2009 | 19100836 |
| comparison of antibody responses in atlantic cod (gadus morhua l.) to vibrio anguillarum, aeromonas salmonicida and francisella sp. | bacterial diseases such as vibriosis, atypical furunculosis and francisellosis, are registered as an increasing problem in cod farming in norway. in order to develop efficient vaccines against diseases it is of interest to investigate if the cod immune system differentiates between various serotypes of vibrio anguillarum and variants of aeromonas salmonicida associated with the diseases by raising specific antibody responses. cod of the same origin were shown to raise significant responses to v. ... | 2009 | 19084603 |
| isolation and functional characterization of neutrophil-like cells, from goldfish (carassius auratus l.) kidney. | neutrophils are one of the first cells to arrive at the site of inflammation and are central cells of innate immunity of vertebrates. upon activation, neutrophils are capable of producing an intense respiratory burst response, they rapidly degranulate in the presence of pathogens, and deploy neutrophil extracellular traps that efficiently remove infections agents. we report on the isolation, cultivation and characterization of neutrophil-like cells isolated from the goldfish kidney. neutrophil-l ... | 2009 | 19046985 |
| the asap1 peptidase of aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family m35) and a major virulence factor. | infections by the bacterium aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. in this study, we showed that asap1, a toxic 19-kda metallopeptidase produced by a. salmonicida subsp. achromogenes, belongs to the group of extracellular peptidases (aeromonas type) (merops id m35.003) of the deuterolysin family of zinc-dependent aspzincin endopeptidases. the structural gene of asap1 was sequenced and found to be highly conserved among gram-negative bacte ... | 2009 | 18952802 |
| expression profiles of cytokines released in intestinal epithelial cells of the rainbow trout, oncorhynchus mykiss, in response to bacterial infection. | to determine whether fish intestinal epithelial cells (iecs) contribute to mucosal immunity, we established a method for isolating iecs from the rainbow trout oncorhynchus mykiss and examined cytokine production in these cells. components of the intestinal epithelium were released by incubation of intestinal pieces with 1mm dithiothreitol (dtt)/ethylenediamine tetraacetic acid (edta). the iec-rich fraction (purity >90%; survival rate approximately 95%) was obtained by centrifugation on a 35%/40% ... | 2009 | 18952122 |
| atypical furunculosis vaccines for atlantic cod (gadus morhua); vaccine efficacy and antibody responses. | atypical furunculosis caused by atypical aeromonas salmonicida, is an emerging problem in farming of atlantic cod (gadus morhua) in norway, and vaccines are needed. atypical a. salmonicida comprises a heterogeneous group of bacteria differing in surface antigens such as the a-layer protein and lipopolysaccharides (lps). except for one of the experimental oil-adjuvanted whole cell vaccines based on various isolates they all resulted in moderate protection. no clear correlation between vaccine eff ... | 2008 | 18951938 |
| isolation of mannose-binding c-type lectin from sea lamprey (petromyzon marinus) plasma and binding to aeromonas salmonicida. | the sea lamprey (petromyzon marinus) is a parasitic cartilaginous fish of the north american great lakes and a predator of many bony fish species of commercial importance to the fishing industry. mannose-binding c-type lectin (mbl) was isolated by mannan-agarose affinity chromatography from sea lamprey plasma. mannose-binding lectin has not before been identified and quantitated in the plasma of this sea lamprey species. the affinity-purified and 2-me reduced lamprey mbl showed two bands of 35kd ... | 2008 | 18937980 |
| preliminary studies of the effects of sulfonamides on fish and bacterium salmonicida. | 1947 | 18918643 | |
| chinese herbs (astragalus radix and ganoderma lucidum) enhance immune response of carp, cyprinus carpio, and protection against aeromonas hydrophila. | the effect of chinese herbs (astragalus radix and ganoderma lucidum) on immune response of carp was investigated. fish were fed diets containing astragalus (0.5%), ganoderma (0.5%) and combination of two herbs (astragalus 0.5% and ganoderma 0.5%) for 5 weeks. other groups of fish were vaccinated (i.p.) against aeromonas hydrophila/aeromonas salmonicida (shering plough, essex, u.k.) at the beginning of the experiment and fed the same diets as described above. control fish (negative control) and f ... | 2009 | 18817878 |
| expressed sequence tags (ests) from immune tissues of turbot (scophthalmus maximus) challenged with pathogens. | the turbot (scophthalmus maximus; scophthalmidae; pleuronectiformes) is a flatfish species of great relevance for marine aquaculture in europe. in contrast to other cultured flatfish, very few genomic resources are available in this species. aeromonas salmonicida and philasterides dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the turbot industry. little is known about the molecular mechanisms for disease resistance and host-pathogen interactions in ... | 2008 | 18817567 |
| the genome of aeromonas salmonicida subsp. salmonicida a449: insights into the evolution of a fish pathogen. | aeromonas salmonicida subsp. salmonicida is a gram-negative bacterium that is the causative agent of furunculosis, a bacterial septicaemia of salmonid fish. while other species of aeromonas are opportunistic pathogens or are found in commensal or symbiotic relationships with animal hosts, a. salmonicida subsp. salmonicida causes disease in healthy fish. the genome sequence of a. salmonicida was determined to provide a better understanding of the virulence factors used by this pathogen to infect ... | 2008 | 18801193 |
| surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006. | the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ... | 2008 | 18786028 |
| polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida. | the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ... | 2008 | 18783128 |
| identification of iron regulated genes in the fish pathogen aeromonas salmonicida subsp. salmonicida: genetic diversity and evidence of conserved iron uptake systems. | in this study we applied the fur titration assay (furta) to isolate iron regulated genes in aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish. we have identified genes for siderophore biosynthesis and for ferri-siderophore transport, some of which have been previously described in this species. a gene for a hitherto uncharacterized functional hemin receptor hute was identified, but its inactivation did not affect significantly the use of hemin as sole iron sou ... | 2009 | 18774242 |
| contribution of food deprivation to the immune response in rainbow trout (oncorhynchus mykiss) vaccinated against cryptobia salmositica and aeromonas salmonicida. | the aims of the present study were to determine (a) the effectiveness of an attenuated live cryptobia salmositica vaccine; (b) the effects of food deprivation on the immune response and its duration in rainbow trout (oncorhynchus mykiss) immunised with a live c. salmositica vaccine or with a killed aeromonas salmonicida vaccine. the fish were divided into three groups (i, ii and iii; 14 fish per group), those in groups i and ii were under food deprivation (0.40% of body weight), while group iii ... | 2008 | 18669247 |
| isolation of putative probionts from cod rearing environment. | survival problems are encountered at early stages of intensive fish rearing and antibiotics are widely used to remedy the situation. probiotics may provide a potential alternative method to protect larvae from opportunistic and pathogenic bacteria and promote a balanced environment. this study was designed to search for new probiotics to target this critical period in cod rearing. potential probionts were selected from the natural microbiota of cod aquacultural environment. the selection was bas ... | 2008 | 18599226 |
| a micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in atlantic canada. | a micro-dilution technique with changes in optical density (od) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 microg otc (oxytetracycline)ml(-1) was applied to determine growth inhibition (gi) in mixed bacterial strains cultured from samples of marine sediments and salmon feed pellets. growth of control cultures (aeromonas salmonicida) was inhibited at all otc concentrations. some feed pellet samples and under-cage sediments from salmon aquac ... | 2008 | 18589456 |
| insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. | wastewater treatment plants (wwtps) are a reservoir for bacteria harbouring antibiotic resistance plasmids. to get a comprehensive overview on the plasmid metagenome of wwtp bacteria showing reduced susceptibility to certain antimicrobial drugs an ultrafast sequencing approach applying the 454-technology was carried out. one run on the gs 20 system yielded 346,427 reads with an average read length of 104 bases resulting in a total of 36,071,493 bases sequence data. the obtained plasmid metagenom ... | 2008 | 18586057 |
| identification of heme uptake genes in the fish pathogen aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish, can use heme as the sole iron source. we applied the fur titration assay to isolate a cluster including six genes hutazxbcd that showed similarity to heme uptake genes of other gram-negative bacteria, and three genes orf123 of unknown function. the spatial organization of these nine genes, arranged in five transcriptional units, was similar to that of a homologous cluster in a. hydrophila. when a tonb system w ... | 2008 | 18535817 |
| development of multiplex pcr assay for simultaneous detection of five bacterial fish pathogens. | a multiplex polymerase chain reaction (pcr) method was designed for the simultaneous detection of the five major fish pathogens, aeromonas hydrophila, aeromonas salmonicida subsp. salmonicida, flavobacterium columnare, renibacterium salmoninarum, and yersinia ruckeri. each of the five pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. the detection limits of the multiplex pcr was in the range of 2, 1, 1, 3, and 1cfu for a. hydrophila, a. salmo ... | 2008 | 18499358 |
| aeromonas salmonicida peritonitis after eating fish in a patient undergoing capd. | 2008 | 18474929 | |
| distribution of small plasmids in aeromonas salmonicida subsp. salmonicida strains isolated from nw spain and portugal: evidence of clonality in strains isolated from turbot, psetta maxima (l.). | 2008 | 18471103 | |
| identification of mhc class iibeta resistance/susceptibility alleles to aeromonas salmonicida in brook charr (salvelinus fontinalis). | pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (mhc) genes but have been tested for very few vertebrates. in this study, we first investigate by sscp (single strand conformational polymorphism) the diversity found at the mhc class iibeta gene in a population of brook charr (salvelinus fontinalis) from the rupert river (québec, canada). secondly, to explore the survival performances conferred by sp ... | 2008 | 18455800 |
| comparison of atypical furunculosis vaccines in spotted wolffish (anarhicas minor o.) and atlantic halibut (hippoglossus hippoglossus l.). | atypical furunculosis caused by atypical aeromonas salmonicida is a problem in farming of atlantic halibut (hippoglossus hippoglossus) and spotted wolffish (anarhicas minor) in norway, and vaccines for marine fish species are not available. susceptibility to atypical a. salmonicida infection and efficacy of furunculosis vaccines in various fish species indicate that the host responses differ. here, spotted wolffish was shown to be highly susceptible to intraperitoneal challenge with atypical a. ... | 2008 | 18450337 |
| cloning, binding properties, and tissue localization of rainbow trout (oncorhynchus mykiss) ladderlectin. | the present paper describes the primary structure, expression and immunohistochemical localization of rainbow trout ladderlectin (rtll), a multimeric serum lectin that binds sepharose and lps of aeromonas salmonicida. two rainbow trout cdnas (504 and 546bp) and a genomic sequence (2kb) were amplified using ladderlectin-specific primers. the sequences were identified as group vii mannose-binding c-type lectins from predicted amino acid sequences and showed highest identity with the atlantic salmo ... | 2008 | 18407519 |
| transferable, multiple antibiotic and mercury resistance in atlantic canadian isolates of aeromonas salmonicida subsp. salmonicida is associated with carriage of an inca/c plasmid similar to the salmonella enterica plasmid psn254. | the aim of this study was to examine the molecular basis for multiple antibiotic and mercury resistance in canadian isolates of aeromonas salmonicida subsp. salmonicida. | 2008 | 18375380 |
| identification of siderophore biosynthesis genes essential for growth of aeromonas salmonicida under iron limitation conditions. | aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, produces a catechol-type siderophore under iron-limiting conditions. in this study, the fur titration assay (furta) was used to identify a cluster of six genes, asbg, asbf, asbd, asbc, asbb, and asbi, encoding proteins similar to components of the siderophore biosynthetic machinery in other bacteria. reverse transcriptase pcr analyses showed that this cluster consists of four iron-regulated transcriptional u ... | 2008 | 18296539 |
| quorum sensing signals are produced by aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor. | many pathogens control production of virulence factors by self-produced signals in a process called quorum sensing (qs). we demonstrate that acyl homoserine lactone (ahl) signals, which enable bacteria to express certain phenotypes in relation to cell density, are produced by a wide spectrum of aeromonas salmonicida strains. all 31 typical strains were ahl producers as were 21 of 26 atypical strains, but on a strain population basis, production of virulence factors such as protease, lipase, a-la ... | 2007 | 18286807 |
| recombinant production and characterization of the carbohydrate recognition domain from atlantic salmon c-type lectin receptor c (sclrc). | the atlantic salmon c-type lectin receptor c (sclrc) locus encodes a potential oligomeric type ii receptor. c-type lectins recognize carbohydrates in a ca(2+)-dependent manner through structurally conserved, yet functionally diverse, c-type lectin-like domains (ctlds). many conserved amino acids in animal ctlds are present in sclrc, with the notable exception of an asparagine crucially involved in ca(2+)- and carbohydrate-binding, which is tyrosine in sclrc. sclrc also contains six cysteines tha ... | 2008 | 18272393 |
| the involvement of aeromonas salmonicida virulence factors in bacterial translocation across the rainbow trout, oncorhynchus mykiss (walbaum), intestine. | the pathogenic bacterium aeromonas salmonicida is the causative agent of furunculosis, a lethal disease in salmonids. the mode of lateral transmission has not been conclusively defined, but a. salmonicida is able to translocate across the intestinal epithelium of salmonids, making the intestinal route a probable candidate. this study investigated some of the virulence mechanisms used by the bacteria to promote translocation. intestinal segments were placed in modified ussing chambers to investig ... | 2008 | 18234022 |
| contribution of type iv pili to the virulence of aeromonas salmonicida subsp. salmonicida in atlantic salmon (salmo salar l.). | aeromonas salmonicida subsp. salmonicida, a bacterial pathogen of atlantic salmon, has no visible pili, yet its genome contains genes for three type iv pilus systems. one system, tap, is similar to the pseudomonas aeruginosa pil system, and a second, flp, resembles the actinobacillus actinomycetemcomitans flp pilus, while the third has homology to the mannose-sensitive hemagglutinin pilus of vibrio cholerae. the latter system is likely nonfunctional since eight genes, including the gene encoding ... | 2008 | 18212071 |
| temperate bacteriophage phio18p from an aeromonas media isolate: characterization and complete genome sequence. | a group of 74 aeromonas isolates from surface water of three ponds in bielefeld, germany was screened for prophage induction after uv irradiation. the phage phio18p was induced from the aeromonas media isolate o18. phio18p belongs to the myoviridae phage family. the complete nucleotide sequence of the double stranded dna genome of bacteriophage phio18p consists of 33,985 bp. the genome has 5' protruding cohesive ends of 16 bases. on the phio18p genome 46 open reading frames (orfs) were identifie ... | 2008 | 18096197 |
| cortisol response and immune-related effects of atlantic salmon (salmo salar linnaeus) subjected to short- and long-term stress. | it is generally considered that stress causes decreased immune function in fish. in this study we examined in atlantic salmon (salmo salar linnaeus) the effects of both short- (a single 15s out of water) and long-term (4 weeks of daily handling 15s out of water) stress on plasma cortisol (free and total) and glucose levels, expression of interleukin-1beta (il-1beta) and survival of head kidney (hk) macrophages under culture with aeromonas salmonicida. in the short-term study, samples were collec ... | 2008 | 18065240 |
| antibacterial action of l-amino acid oxidase from the skin mucus of rockfish sebastes schlegelii. | l-amino acid oxidase (lao) shows broadly antibacterial activity against gram-positive and gram-negative bacteria by h(2)o(2) generated in the oxidative process of l-amino acids. however, lao (termed ssap) isolated from the rockfish sebastes schlegelii skin mucus acted selectively on gram-negative bacteria. therefore, this study was undertaken to clarify the antibacterial action of ssap as compared with h(2)o(2). ssap inhibited potently the growth of aeromonas salmonicida, photobacterium damselae ... | 2008 | 18055243 |
| in vitro studies of lactobacillus delbrueckii subsp. lactis in atlantic salmon (salmo salar l.) foregut: tissue responses and evidence of protection against aeromonas salmonicida subsp. salmonicida epithelial damage. | probiotic bacteria increase the host health status and protect mucosal tissue against pathogen-caused damage in mammalian models. using an in vitro (intestinal sac) method this study aimed to address (a) the in vitro ability of lactobacillus delbrueckii subsp. lactis to remain in the gastrointestinal tract of atlantic salmon (salmo salar l.) and (b) its ability to prevent cellular damage caused by successive incubation with aeromonas salmonicida subsp. salmonicida the causative agent of furuncul ... | 2008 | 18054448 |
| development of a pcr protocol for the detection of aeromonas salmonicida in fish by amplification of the fsta (ferric siderophore receptor) gene. | the aims of the study were to evaluate a new pcr protocol designed to detect aeromonas salmonicida in fish tissues and to develop a non-destructive method for the diagnosis of furunculosis. a set of primers (fer3, fer4), flanking a fragment of the fsta gene (coding for the ferric-siderophore receptor) was designed, showing to be sensitive and specific. when compared to pcr methods previously reported, the new protocol recognized all the 69 a. salmonicida strains evaluated, with no cross-reaction ... | 2008 | 18035507 |
| isolation of aeromonas salmonicida from sea lamprey (petromyzon marinus) with furuncle-like lesions in lake ontario. | for the past six decades, parasitic sea lampreys (petromyzon marinus) have caused devastating losses to salmonid fisheries in the great lakes. to reduce the number of sea lampreys, the great lakes fishery commission began a large-scale program based on trapping male sea lampreys, sterilizing them, and releasing sterile males back into streams to compete with fertile males for spawning females. the transfer of lampreys among lakes can potentially lead to the transfer of various pathogens, and thi ... | 2007 | 17984256 |
| analysis of type iii effector genes in typical and atypical aeromonas salmonicida. | 2007 | 17958615 | |
| the development of probiotics for the control of multiple bacterial diseases of rainbow trout, oncorhynchus mykiss (walbaum). | jb-1 and gc2, which were equated with bacillus sp. and aeromonas sobria respectively, were recovered from the digestive tract of rainbow trout, oncorhynchus mykiss and ghost carp, cyprinus sp. respectively, and demonstrated effectiveness as probiotics for the control of infections caused by aeromonas salmonicida, lactococcus garvieae, streptococcus iniae, vibrio anguillarum, vibrio ordalii and yersinia ruckeri. when administered to rainbow trout (average weight = 12 g) for 14 days in feed dosed ... | 2007 | 17850573 |
| the early response of atlantic salmon (salmo salar) macrophages exposed in vitro to aeromonas salmonicida cultured in broth and in fish. | aeromonas salmonicida is a fish pathogen that causes furunculosis. virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. it is known that a. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. therefore, in this study, the early macrophage respon ... | 2008 | 17825909 |
| function and molecular architecture of the yersinia injectisome tip complex. | by quantitative immunoblot analyses and scanning transmission electron microscopy (stem), we determined that the needle of the yersinia enterocolitica e40 injectisome consists of 139 +/- 19 yscf subunits and that the tip complex is formed by three to five lcrv monomers. a pentamer represented the best fit for an atomic model of this complex. the n-terminal globular domain of lcrv forms the base of the tip complex, while the central globular domain forms the head. hybrids between lcrv and its ort ... | 2007 | 17697254 |
| carbohydrate analysis and serological classification of typical and atypical isolates of aeromonas salmonicida: a rationale for the lipopolysaccharide-based classification of a. salmonicida. | the cell envelope of aeromonas salmonicida contains a lipopolysaccharide (lps) essential for the physical integrity and functioning of bacterial cell membrane. using a recently developed in-source fragmentation technique, we screened 39 typical and atypical isolates of a. salmonicida and established their o-chain polysaccharide structure by capillary electrophoresis-mass spectrometry (ce-ms), compositional and linkage analyses and comparison to the previously determined o-chain polysaccharide st ... | 2007 | 17658271 |
| aeromonas exoenzyme t of aeromonas salmonicida is a bifunctional protein that targets the host cytoskeleton. | type iii protein secretion has been shown recently to be important in the virulence of the fish pathogen aeromonas salmonicida. the adp-ribosylating toxin aeromonas exoenzyme t (aext) is one effector protein targeted for secretion via this system. in this study, we identified muscular and nonmuscular actin as substrates of the adp-ribosylating activity of aext. furthermore, we show that aext also functions as a gtpase-activating protein (gap), displaying gap activity against monomeric gtpases of ... | 2007 | 17656370 |
| enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (oncorhynchus mykiss). | we analysed the effect of probiotic strains on the cellular and humoral immune responses of rainbow trout (oncorhynchus mykiss), and their capacity to prevent furunculosis during a challenge trial. probiotic strains (lactococcus lactis ssp. lactis clfp 100, leuconostoc mesenteroides clfp 196, and lactobacillus sakei clfp 202) were administered orally to fish for 2 weeks at 10(6) cfu g(-1) of feed. in comparison to untreated control fish, the phagocytic activity of head kidney leukocytes and the ... | 2007 | 17645738 |
| further characterization of a type iii secretion system (t3ss) and of a new effector protein from a clinical isolate of aeromonas hydrophila--part i. | a type iii secretion system (t3ss)-associated cytotoxin, aext, with adp-ribosyltransferase activity and homology to pseudomonas aeruginosa bifuncational toxins exot/s, was recently identified from a fish pathogen aeromonas salmonicida. in this study, we reported the molecular characterization of an aext-like toxin gene (designated as aexu) from a diarrheal isolate ssu of a. hydrophila. the aexu gene was 1539bp in length and encoded a protein of 512 amino acid (aa) residues. the nh(2)-terminus of ... | 2007 | 17644303 |
| detection of the causative agent of furunculusis, aeromonas salmonicida in salmonids of the krka river. | in this paper we describe the bacterial community associated with salmonids from the krka river. diversity analysis demonstrated that majority of the recovered bacteria were related to aeromonadaceae group. bacterial analysis also revealed the presence of shigella spp. and pseudomonas fluorescens. isolation of aeromonas salmonicida from trout, presents first isolation of this bacteria croatian rivers. | 2008 | 17624808 |
| recombinant prostate-specific antigen proaerolysin shows selective protease sensitivity and cell cytotoxicity. | native proaerolysin is a channel-forming bacterial protoxin that binds to cell-surface receptors and then is activated by furin or furin-like proteases. we genetically engineered proaerolysin by replacing the furin-cleavage sequence with a prostate-specific antigen-selective sequence. the recombinant modified proaerolysin was expressed and purified from aeromonas salmonicida in good yields and purity. recombinant modified proaerolysin had no furin sensitivity and markedly increased prostate-spec ... | 2007 | 17581303 |
| atypical aeromonas salmonicida infection in naturally- and laboratory-challenged farmed haddock, melanogrammus aeglefinus (l.). | 2007 | 17501741 | |
| protection against atypical aeromonas salmonicida infection in carp (cyprinus carpio l.) by oral administration of humus extract. | humic substances are formed during the decomposition of organic matter in humus, and are found in many natural environments in which organic materials and microorganisms have been present. in the present study, oral administration of humus extract to common carp (cyprinus carpio l.) induced effective protection against experimental atypical aeromonas salmonicida infection. mortality of fish and development of skin lesions such as hemorrhages and ulcers were significantly suppressed in carp treat ... | 2007 | 17485929 |
| global trade in ornamental fish from an australian perspective: the case for revised import risk analysis and management strategies. | over 1 billion ornamental fish comprising more than 4000 freshwater and 1400 marine species are traded internationally each year, with 8-10 million imported into australia alone. compared to other commodities, the pathogens and disease translocation risks associated with this pattern of trade have been poorly documented. the aim of this study was to conduct an appraisal of the effectiveness of risk analysis and quarantine controls as they are applied according to the sanitary and phytosanitary ( ... | 2007 | 17485126 |
| cytokine expression in the intestine of rainbow trout (oncorhynchus mykiss) during infection with aeromonas salmonicida. | gene expression of a number of cytokines in the intestine of rainbow trout (oncorhynchus mykiss) was investigated after challenge with a pathogenic strain of aeromonas salmonicida. fish were exposed to a. salmonicida by immersion in a bacterial suspension (bath challenge) and tissue samples of the distal and proximal intestine were collected at days 0, 2, 4, 6 and 8 post-exposure. head kidney tissue was also collected to assess the effect in a systemic immune tissue. a classic profile of pro-inf ... | 2007 | 17434320 |
| bacterial-binding activity and plasma concentration of ladderlectin in rainbow trout (oncorhynchus mykiss). | soluble, defense lectins bind conserved microbial patterns leading to pathogen opsonization, enhanced phagocytosis and activation of complement. these immune functions, however, vary widely among individuals due to genetic and acquired differences affecting binding capacity or plasma concentration. most evidence for the defensive function of soluble lectins is based on mammals, but several functionally homologous, but less well-characterized, lectins have been identified in fish. in this study, ... | 2007 | 17383895 |
| vaccination against atypical furunculosis and winter ulcer disease of fish. | atypical furunculosis is a problem in farming of salmonids and various other fish species caused by a heterogeneous group of atypical aeromonas salmonicida strains. winter ulcer is a disease of salmonids and cod caused by moritella viscosa, but a number of fish species are susceptible to the infection. vaccines are available against atypical furunculosis of salmonids, but their efficacy is dependent on the characteristics of the infective strain. vaccines for non-salmonid fish are currently not ... | 2007 | 17367897 |
| quantitative detection of aeromonas salmonicida in fish tissue by real-time pcr using self-quenched, fluorogenic primers. | in this study a real-time pcr assay using self-quenched primers labelled with a single fluorophore for the detection of aeromonas salmonicida was developed. probe specificity was confirmed by amplification of 16 a. salmonicida strain templates and by the lack of a pcr product with 26 non-a. salmonicida strains. with a pure culture of a. salmonicida, the assay was linear over a range of 0.5 pg to 50 ng and was able to detect 16 c.f.u. per reaction. a similar sensitivity was observed in dna extrac ... | 2007 | 17314361 |
| a stochastic modelling approach to describing the dynamics of an experimental furunculosis epidemic in chinook salmon, oncorhynchus tshawytscha (walbaum). | a susceptible-infected-removed (sir) stochastic model was compared to a susceptible-latent-infectious-removed (slir) stochastic model in terms of describing and capturing the variation observed in replicated experimental furunculosis epidemics, caused by aeromonas salmonicida. the epidemics had been created by releasing a single infectious fish into a group of susceptible fish (n = 43) and progress of the epidemic was observed for 10 days. this process was replicated in 70 independent groups. th ... | 2007 | 17298564 |
| aeromonas salmonicida toxin aext has a rho family gtpase-activating protein domain. | the n terminus of the aeromonas salmonicida adp-ribosylating toxin aext displays in vitro gtpase-activating protein (gap) activity for rac1, cdc42, and rhoa. hela cells transfected with the aext n terminus exhibit rounding and actin disordering. we propose that the aeromonas salmonicida aext toxin is a novel member of the growing family of bacterial rhogaps. | 2007 | 17237181 |
| occurrence of n-acyl-l-homoserine lactones in extracts of some gram-negative bacteria evaluated by gas chromatography-mass spectrometry. | acylated homoserine lactones (ahls) are self-generated signal molecules that mediate population density-dependent gene expression (quorum sensing) in a variety of gram-negative bacteria. these signal molecules diffuse from bacterial cells and accumulate in the medium as a function of cell growth. in selected foods ahls contribute to product spoilage. as different bacterial species produce ahl analogs that differ in length of the n-acyl chain, ranging from 4 to 14 carbons and in the substitution ... | 2007 | 17207763 |