Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| pay review body: body blow or a minor injury? | 2006 | 1489700 | |
| mechanisms of ion and acid-base regulation at the gills of freshwater fish. | this review examines the branchial mechanisms utilized by freshwater fish to regulate internal acid-base status and presents a model to explain the underlying basis of the compensatory processes. rainbow trout, oncorhynchus mykiss, and brown bullhead, ictalurus nebulosus, were examined under a variety of experimental treatments which induced respiratory and metabolic acid-base disturbances. acid-base regulation was achieved by appropriate adjustments of na+ and cl- net fluxes across the gills wh ... | 2006 | 1500882 |
| colotomy with minimum resection for advanced irreducible intussusception. | 2006 | 1501020 | |
| erythropoietin use in the critical care setting. | biotechnology products signify a major advancement in our world today. products resulting from biotechnology will revolutionize how health care is delivered. one of these technologic breakthroughs is recombinant human erythropoietin (epoetin). its impact on the delivery of care to the anemic renal patient is changing the roles of nurses who provide care for these patients. epoetin alfa has virtually eliminated the necessity of transfusions in the renal patient population, while simultaneously im ... | 1992 | 1524935 |
| occurrence of a capsule in aeromonas salmonicida. | aeromonas salmonicida grown in a medium with excess glucose as carbon source produces both capsular and exocellular polysaccharides. the capsular polysaccharide is composed of glucose, mannose, rhamnose, n-acetylmannosamine and mannuronic acid in the molar ratios of approximately 5:3:0.75:2:1. the extracellular polysaccharide is similarly constituted, but in the molar ratios of approximately 4.75:10.5:1.5:2:1. the capsular and exocellular polysaccharides did not cross-react with monoclonal antib ... | 1992 | 1526444 |
| relationship between intracellular ph and tension development in resting ventricular muscle and myocytes. | simultaneous measurements of intracellular ph (phi) and tension development were made in resting cat papillary muscles and resting ventricular myocytes (cat, guinea pig). ph microelectrodes and the fluorescent indicator carboxy-seminaphthorhodafluor-1 (snarf-1) were used to measure phi in muscles and myocytes, respectively. phi-induced changes in isometric muscle tension and myocyte length were elicited by variations in pco2, hco3-, and [nh4cl]. increased phi elevated resting tension and decreas ... | 1992 | 1539624 |
| effect of monensin and supplemental hay on ruminal 3-methylindole formation in adult cows after abrupt change to lush pasture. | the effect of feeding monensin, with or without dry hay plus wilted forage, on ruminal formation of 3-methylindole (3mi) was investigated in pastured cattle. eighty-two cows were allotted to 3 groups. cows of group-1 served as controls and were given a daily energy supplement (1 kg/head) without monensin for 1 day before and for 7 days after being allowed access to lush pasture. cows of groups 2 and 3 were given the same daily energy supplement, which also contained monensin (200 mg/kg of supple ... | 1992 | 1539904 |
| amoxycillin in the control of furunculosis in atlantic salmon parr. | the efficacy of amoxycillin in the control of laboratory induced aeromonas salmonicida infection in atlantic salmon parr was investigated. when given in the diet at a dose rate of 80 mg per kg bodyweight it was effective against both a moderate and severe challenge (with mortality rates in untreated groups of 75 per cent and 45 per cent). at 40 mg per kg it was effective against the moderate challenge only. the plasma levels in these regimens were 1.25 micrograms per ml and 0.3 to 0.6 micrograms ... | 1992 | 1546434 |
| nurse prescribing: how and when. | legislation for nurse prescribing now seems destined to be implemented in october 1993, writes judith moreton who is on the nurse prescribers formulary subcommittee. a private members bill from chislehurst mp roger sims, received its second reading on the 31 january. it has full government support and should become law in this session of parliament. | 2005 | 1559831 |
| executive empowerment enhances profession. | 2006 | 1560269 | |
| reform and restructuring in nursing education: a necessity for nursing's future in florida. | 2006 | 1563527 | |
| animal rights/liberation philosophy. | 2006 | 1563603 | |
| pulsatile growth hormone secretion during the breeding season in male reindeer and its association with hypophagia and weight loss. | male deer reduce food intake and lose weight during the breeding season (rut) in autumn. growth hormone (gh) is lipolytic in ruminants; therefore, pulsatile gh secretion was measured in three young reindeer (rangifer tarandus tarandus) bulls during the natural period of weight loss during rut. blood samples were withdrawn through a jugular cannula every 20 min for 16 hr on 5 occasions from late summer to early winter in unanaesthetised animals. the deer were group fed a pelleted concentrate rati ... | 1992 | 1563616 |
| dna probe for aeromonas salmonicida. | a dna fragment that is specific to aeromonas salmonicida has been isolated from a genomic dna library by differential hybridization. the specificity of this fragment as a dna probe for a. salmonicida was shown by hybridization against reference strains and clinical isolates of a. salmonicida, related aeromonads, and species from several other bacterial genera. the sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two a. salmonicida cells. | 1992 | 1575477 |
| [the treatment concept and results of peri-/sub-prosthetic fractures]. | the treatment of choice in ipsilateral femoral shaft fractures in patients with hip prosthesis depends on the location of the fracture lines and the observation of loosening of the prosthesis. either a change of prosthesis, if necessary in combination with refixation of the greater trochanter or simple open reduction with internal fixation (asif) along with autologous and/or homologus spongiosa are the used techniques. the rate of complications in peri- or subprothetic fractures is high. lack of ... | 2006 | 1590026 |
| role of catecholamines in the courtship behavior of male ring doves. | the role of catecholamines in the expression of male courtship behavior in ring doves was examined using central administration of pharmacological agents. males treated with 6-hydroxydopamine or u-14,624, which depleted norepinephrine (ne) levels in the preoptic-hypothalamic area, showed increased levels of bow-coo and nest-coo displays. conversely, males treated with tyramine or desipramine, which elevated ne levels in the preoptic-hypothalamic area, showed decreased levels of bow-coo and nest- ... | 2006 | 1594642 |
| [mono-arthritis of uncertain etiology--a follow-up study]. | 78 patients in whom the cause of a monoarticular arthritis remained unclear after an initial workup were contacted for a follow-up interview (and an additional clinical examination in 64 cases) after 6 to 11 years (mean 8 years). the mean age at the onset of symptoms was 39 years, with 51% of the patients presenting in the range between 20 and 40 years. there was a slight male preponderance (56%). large joints, mainly the knee, and less often the wrist, ankle or hip, were affected in 79%. finger ... | 1992 | 1594908 |
| a comparison of total and specific immunoglobulin levels in healthy atlantic salmon (salmo salar l.) and in salmon naturally infected with aeromonas salmonicida subsp. achromogenes. | healthy atlantic salmon and salmon with a history of chronic natural aeromonas salmonicida subsp. achromogenes infection were compared with respect to total serum protein and the concentration and specificity of serum immunoglobulin. the immunoglobulin level was measured using competitive elisa and the specific antibody activity against aeromonas salmonicida subsp. achromogenes was measured using double sandwich elisa. significant elevation of serum protein and immunoglobulin concentration was o ... | 1992 | 1604799 |
| glycosidase activities in gingival crevicular fluid in subjects with adult periodontitis or gingivitis. | specific glycosidase activities were determined in samples of gingival crevicular fluid (gcf) collected from eight predetermined sites in two groups, each of 20 adult patients, with either gingivitis or periodontitis. the total activities (as units of enzyme activity per sample) of alpha-l-fucosidase, sialidase, beta-n-acetylglucosaminidase, beta-galactosidase, beta-glucosidase and alpha-glucosidase were significantly greater in the periodontitis group. in contrast, the total beta-mannosidase an ... | 2006 | 1610303 |
| toxicity of aeromonas salmonicida cells to atlantic salmon salmo salar peritoneal macrophages. | several strains of aeromonas salmonicida were toxic to cultured peritoneal macrophages of atlantic salmon, salmo salar, in minimum doses of 5 x 10(6) cfu, whereas other strains were not. there was no correlation between cytotoxicity and in vivo virulence of the bacteria, the presence or absence of the two major surface components of a. salmonicida (namely, the a-layer and the o-polysaccharide chain of the lps) nor, finally, with the ability of the strains to produce the following enzymes in vitr ... | 1992 | 1618355 |
| evaluation of the child with idiopathic mental retardation. | a review of the advances in diagnostic techniques for evaluation of children with idiopathic mental retardation is presented. the current status of the use of clinical genetics, cytogenetics, molecular genetics, and neuroimaging in evaluating children with mental retardation is emphasized. special attention is given to the evaluation of children with mental retardation and "autism" or "cerebral palsy." | 2006 | 1635812 |
| preliminary results of intensive therapy of children and adolescents with acute nonlymphocytic leukemia--a childrens cancer study group report. | 2002 | 1691130 | |
| developmental cycle-specific host-free rna synthesis in chlamydia spp. | the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ... | 1990 | 1698176 |
| binding of laminin and fibronectin by the trypsin-resistant major structural domain of the crystalline virulence surface array protein of aeromonas salmonicida. | the surface of aeromonas salmonicida is covered by a tetragonal paracrystalline array (a-layer) composed of a single protein (a-protein, mr = 50,778). this array is a virulence factor. cells containing a-layer and isolated a-layer sheets specifically bound laminin and fibronectin with high affinity. binding by cells was inactivated by selective removal of a-layer at ph 2.2, and neither isogenic a-layer-deficient a. salmonicida mutants nor tetragonal paracrystalline array producing aeromonas hydr ... | 1992 | 1730607 |
| isolation and characterization of turbot (scophtalmus maximus)-associated bacteria with inhibitory effects against vibrio anguillarum. | more than 400 isolates from the intestine and the external surface of farmed scophtalmus maximus as well as from fish food and hatchery water were screened for inhibitory effects against the fish pathogen vibrio anguillarum hi 11345 and seven other fish pathogens. the bacteria with inhibitory effects were then characterized with regard to their sites of colonization, especially the intestinal regions and sites within each region. of the total number of bacterial isolates from the intestine, 28% ... | 1991 | 1768092 |
| survival of the fish pathogen aeromonas salmonicida in seawater. | survival of aeromonas salmonicida in natural (non-sterile) seawater, as determined from colony counts on marine agar, was found to be influenced by the presence of potentially inhibitory organisms, i.e., acinetobacter, aeromonas hydrophila, chromobacterium, escherichia coli, flavobacterium and pseudomonas, and their metabolites. yet, samples, thought to be devoid of culturable a. salmonicida, were found to contain cells, which were filterable through 0.22 and 0.45 microns millipore millex porosi ... | 1991 | 1769545 |
| cyanide antagonism in a rodent model. | 1991 | 1805737 | |
| in vitro susceptibility of the fish pathogen aeromonas salmonicida to flumequine. | the activity of the fluoroquinolone flumequine was investigated against the fish pathogen aeromonas salmonicida and was compared with that of oxolinic acid. flumequine was more active than oxolinic acid in terms of its mic against oxolinic acid-resistant isolates of a. salmonicida and was as active as oxolinic acid against susceptible isolates. in contrast to oxolinic acid, flumequine was bactericidal, with only 1% of the bacteria surviving 6 h of exposure to the drug at concentrations slightly ... | 1991 | 1810198 |
| production of colony-stimulating factors during pneumonia caused by chlamydia trachomatis. | the colony-stimulating factors (csfs) are cytokines involved in the production, differentiation, and activation of host phagocytes. during murine infection with chlamydia trachomatis (mopn), plasma csf levels increased in euthymic (nu/+) and athymic (nu/nu) balb/c mice. levels declined later in infection, with the nu/+ mice resolving the infection but the nu/nu mice succumbing by day 16. either live or heat-killed chlamydia organisms could induce csf increases on day 7 postchallenge in nu/+ mice ... | 1991 | 1828791 |
| the arcuate line of the rectus sheath--does it exist? | the lower halves of the anterior abdominal wall of 40 human specimens of both sexes and various ages were studied. the anatomical features seen were widely variable and quite different from the conventional picture. the fibres of the posterior lamina of the internal oblique and transversus abdominis aponeuroses gradually shift from the posterior to the anterior rectus sheath starting from the level of the umbilicus down to the level of the symphysis pubis. thus, these anterior laminae gradually ... | 1991 | 1828798 |
| siderophore production by aeromonas salmonicida. | growth under conditions of iron-restriction and the production of siderophores was examined in 21 typical and 14 atypical strains of aeromonas salmonicida. with the exception of one atypical strain, all strains grew and multiplied in the presence of the high-affinity iron chelators ethylenediamine di(o-hydroxyphenylacetic acid), alpha, alpha'-dipyridyl or transferrin. chrome azurol s agar was used to screen bacterial strains growing under these conditions for the production of siderophores. side ... | 1991 | 1830896 |
| isolation and phenotypic characterization of an oxidase-negative aeromonas salmonicida causing furunculosis in coho salmon (oncorhynchus kisutch). | an oxidase-negative aeromonas salmonicida was isolated from coho salmon (oncorhynchus) kisutch) suffering from an epizootic of furunculosis at the state hatchery near belfair, washington. typical, oxidase-positive a. salmonicida was isolated concurrently from the same population of fish. mortality was controlled with medicated feed treatments. evidence supporting the identification of the two types of a. salmonicida is presented. methods for the proper identification of oxidase-negative a. salmo ... | 1991 | 1850808 |
| effect of monocular enucleation or impulse blockage on gamma-aminobutyric acid and cytochrome oxidase levels in neurons of the adult cat lateral geniculate nucleus. | much attention has been paid to the effect of various types of neonatally induced retinal insults on neurons of the cat lateral geniculate nucleus (lgn). little is known about cellular adjustment to functional alterations commencing in the adult. the present study was aimed at examining the effect of monocular enucleation or retinal impulse blockade on mature neurons of the cat lgn and perigeniculate nucleus. in addition to labeling the relay neurons with cytochrome oxidase (co) histochemistry a ... | 1991 | 1851037 |
| [assisted ventilation: prevention of nosocomial infections]. | 2007 | 1853087 | |
| high blood pressure during pregnancy and working conditions among hospital personnel. | the relationship between working conditions and high blood pressure during pregnancy was analysed in a sample of 621 women hospital employees in the paris region over the period 1979-1981. data were collected by interviews during the routine medical visit at the end of postnatal leave. women who had to work standing up for extended periods of time, who had to carry heavy loads or who had to perform heavy cleaning tasks had high blood pressure during their pregnancy more often than women not expo ... | 1991 | 1855606 |
| analysis and pyrolysis of some n-nitrosamino acids in tobacco and tobacco smoke. | a new tobacco-specific nitrosamine, 4-(n-nitrosomethylamino)-4-(3-pyridyl)butyric acid (iso-nnac), has been identified in tobacco, and its structure was confirmed by gas chromatography-mass spectrometry following enrichment of a tobacco extract. the levels of iso-nnac ranged from 0.01 to 0.95 ppm. it does not induce dna repair in primary rat hepatocytes and is inactive as a tumorigenic agent in strain a mice. in order to study the fate of nitrosamino acids during smoking, we spiked cigarettes wi ... | 1991 | 1855899 |
| site-directed mutagenesis of a single tryptophan near the middle of the channel-forming toxin aerolysin inhibits its transfer across the outer membrane of aeromonas salmonicida. | the channel-forming protein aerolysin must cross both the inner and outer bacterial membranes during its secretion from aeromonas hydrophila or from aeromonas salmonicida containing the cloned structural gene. we examined the fate of three mutant proteins in which trp-227, near the middle of the amino acid chain, was replaced with glycine, leucine, or phenylalanine by site-directed mutagenesis. all three proteins crossed the inner membrane and entered the periplasm in the same way as wild-type, ... | 1991 | 1860853 |
| human serum stimulates endothelin-1 synthesis more potently than prostacyclin production by cultured vascular endothelial cells. | human serum stimulated the synthesis of a vasoconstrictive peptide, endothelin-1 (et-1), and a vasodilatory prostanoid, prostacyclin (pgi2), by cultured human umbilical vein endothelial cells in a concentration- and time-dependent manner. incubation in 20% concentration of the serum for 24 h stimulated et-1 synthesis almost six-fold while pgi2 production increased two-fold. in addition, a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (pma), inhibited the serum-induced et-1 produ ... | 1991 | 1865754 |
| a citation analysis of the work of lee b. lusted, md. | 2007 | 1865779 | |
| structure of the tetragonal surface virulence array protein and gene of aeromonas salmonicida. | the paracrystalline surface protein array of the pathogenic bacterium aeromonas salmonicida is a primary virulence factor with novel binding capabilities. the species-specific structural gene (vapa) for this array protein (a-protein) was cloned into lambda gt11 but was unstable when expressed in escherichia coli, undergoing an 816-base pair deletion due to a 21-base pair direct repeat within the gene. however, the gene was stable in cosmid pla2917 as long as expression was poor. a-protein was lo ... | 1991 | 1869553 |
| survival of aeromonas salmonicida in lake water. | the survival of aeromonas salmonicida subsp. salmonicida in lake water was investigated by using a variety of techniques. they included acridine orange epifluorescence, respiration, cell culture, cell revival, flow cytometry, plasmid maintenance, and membrane fatty acid analysis. during a 21-day study, a. salmonicida became nonculturable in sterile lake water samples. flow cytometry and direct microscopy indicated that cells were present. although the nonculturable cells could not be revived, th ... | 1991 | 1872606 |
| health status of salmonids in river systems in natal. iii. isolation and identification of bacteria. | both pathogenic and non-pathogenic bacteria were isolated from fish, both salmonid and non-salmonid, from selected river systems in natal. pasteurella pisicida was isolated for the first time from fish in south africa. the isolation of yersinia ruckeri, aeromonas salmonicida, and edwardsiella tarda were recorded for the first time from fish in natal. a. hydrophila and flexibacter columnaris were found to be widespread throughout the river systems in natal. the streptococcus species which caused ... | 1991 | 1881658 |
| does prestretching stainless-steel wire have any clinical significance? | clinical parameters of prestretch and tension forces placed on stainless-steel wire by 20 oral and maxillofacial surgeons were obtained. unstretched wire was clinically as tough or tougher than prestretched wire of a similar gauge. both prestretched and unstretched wires, twisted to their maximum, loosened when under tension. as the prestretch forces increased, the clinical strength of the wire diminished. | 2007 | 1890520 |
| introduction of vascular smooth muscle cells expressing recombinant genes in vivo. | vascular smooth muscle cells contribute to the formation of atherosclerotic plaques by proliferating in response to vascular injury and releasing growth-promoting factors. because their autocrine and paracrine effects are not fully understood, expression of such growth factor genes in specific cell types in vivo would help to determine their mechanism of action. we describe a method to transfer vascular smooth muscle cells expressing recombinant gene products to localized segments of the arteria ... | 1991 | 1899366 |
| effect of diabetes and aging on carboxymethyllysine levels in human urine. | carboxymethyllysine (cml) has been identified as a modified amino acid that accumulates with age in human lens proteins and collagen. cml may be formed by oxidation of fructoselysine (fl), the amadori adduct formed on nonenzymatic glycosylation of lysine residues in protein, or by reaction of ascorbate with protein under autoxidizing conditions. we proposed that measurements of tissue and urinary cml may be useful as indices of oxidative stress or damage to proteins in vivo. to determine the ext ... | 1991 | 1899406 |
| [a statistical model of evolution with stabilizing selection]. | we consider a population of fixed size and reproducing asexually, evolving in a rugged fitness landscape. selection takes place only via the elimination of individuals with unfit genomes. unfit genotypes are distributed at random in genotypic space. the genetic structure of the population and the speed of genetic drift are explicitly computed in the infinite genome limit. | 2007 | 1913251 |
| atypical characteristics of the salmonid pathogen aeromonas salmonicida. | incubation of aeromonas salmonicida at supra-optimal temperatures, i.e. 30-37 degrees c, resulted in the expression of motility by polar flagella, and changes in sugar fermentation patterns, e.g. loss of acid production from mannitol, loss of the ability to degrade complex molecules (aesculin, dna, elastin and gelatin), and an increase in antibiotic resistance (notably co-trimoxazole). motility was enhanced in cultures grown in brain heart infusion broth supplemented with 18% (w/v) ficoll. | 1991 | 1919508 |
| dynamic computed tomography as an ancillary screening test for traumatic thoracic aortic injuries: a critical review. | while the divergence of opinion on the matter of diagnostic modalities will probably continue, dynamic computed tomography scans should be considered as a necessary study in assessing a possible traumatic thoracic aortic injury. the use of this modality will decrease the need for thoracic angiograms and invariably increase the yield. | 2007 | 1920521 |
| psychological stress-induced increases in noradrenaline release in rat brain regions are attenuated by diazepam, but not by morphine. | by measuring levels of noradrenaline (na) and its major metabolite, 3-methoxy-4-hydroxyphenylethyleneglycol sulfate (mhpg-so4) in the hypothalamus, amygdala and locus coeruleus region, we investigated the effects of diazepam 5.0 mg/kg, morphine 6.0 mg/kg, or naloxone at 5.0 or 10 mg/kg injected sc immediately before stress exposure, on increases in na release caused by psychological stress. psychological stress, wherein rats were exposed to emotional responses which were displayed by other elect ... | 1991 | 1924502 |
| significance of the p210 versus p190 molecular abnormalities in adults with philadelphia chromosome-positive acute leukemia. | we investigated the significance of p210 and p190 molecular abnormalities in 32 adults with philadelphia chromosome (ph)-positive acute leukemia. p210 was detected in 15 patients (47%), p190 in 16 (50%), and both in one (3%). p210 was noted in 11 of 24 patients (46%) with acute lymphocytic leukemia, and in four of eight patients (50%) with acute myelogenous or undifferentiated leukemia. among 29 patients with untreated disease (p210, 14 patients; p190, 15 patients), no significant differences in ... | 2007 | 1932753 |
| society, health, and the nhs. | 2007 | 1932997 | |
| anesthesia for male aesthetic surgery. | the male patient for aesthetic plastic surgery should expect to have a good experience, with few, if any complications from anesthesia. proper planning is essential to the process, from setting up the surgical facility to selection and preparation of patients. anesthetic techniques should be adapted to the needs of each patient, with his safety and comfort the most important consideration. anesthesia for plastic surgery has progressed from simple injection of local anesthetics to sophisticated s ... | 2006 | 1934898 |
| self-acceptance, acceptance of others, and symlog: equivalent measures of the two central interpersonal dimensions? | after 50 hours of small group participation during 9 weeks, 91 young adults rated each same-group member's conduct on symlog's dimensions of dominance, friendliness, and task-orientedness. earlier, they made similar ratings twice, several weeks apart, on separate measures of self-acceptance and acceptance of others. individuals' mean symlog dominance ratings by group peers correlated much more highly with aggregated ratings for self-acceptance (.83) than for other-acceptance (.02), while symlog ... | 2007 | 1939704 |
| surface-disorganized, attenuated mutants of aeromonas salmonicida as furunculosis live vaccines. | a slow-growing, aminoglycoside-resistant mutant and a rapidly-growing pseudo-revertant were isolated from aeromonas salmonicida, the causative agent of salmonid furunculosis. these mutants continued to elicit a variety of classical virulence factors associated with a. salmonicida pathogenesis. they differed morphologically from the wild-type and from one another with respect to a-layer organization, membrane antagonist sensitivity and particularly to aerobic metabolism. both mutants were drastic ... | 1991 | 1961112 |
| plasma cotinine, smoking, and lung cancer in china. | 2007 | 1971071 | |
| failure of chronic glutathione elevation to reduce cytotoxicity produced by exposure to cis-diamminedichloroplatinum(ii), ionizing radiation, or hyperthermia. | chinese hamster ovary (cho) cells cultured in vitro were continuously exposed to increasing concentrations of diethylmaleate (dem). chronic exposure of these cells (designated cho/dem) to 80 microm diethylmaleate resulted in an increase in cystine transport, a decrease in glutathione inhibition of the enzyme gamma-glutamylcysteine synthetase, elevation of intracellular glutathione levels to 4.3 times control, and elevation of glutathione-s-transferase activity by 6.6 times. yet, cho/dem and cont ... | 2007 | 1974822 |
| freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles. | freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ... | 1991 | 1999383 |
| effect of paracrystalline protein surface layers on predation by bdellovibrio bacteriovorus. | we determined that paracrystalline protein surface arrays (s layers) protected gram-negative eubacteria from predation by bdellovibrio bacteriovorus. aquaspirillum serpens vha and mw5 and aquaspirillum sinuosum were resistant to predation by b. bacteriovorus 6-5-s when fully covered by their s layers. the s layer of aeromonas salmonicida a449 protected the cells from predication by b. bacteriovorus 109j. a predacious, plaque-forming vibrio that lysed an s-layer- variant of caulobacter crescentus ... | 1991 | 2007549 |
| allergy to n,n-dimethyl-p-toluidine in dental materials. | 2007 | 2040161 | |
| improvement in anemia by recombinant human erythropoietin in patients with myelodysplastic syndrome and aplastic anemia. | 1991 | 2040187 | |
| intraperitoneal chemotherapy. | 2007 | 2042064 | |
| opsonizing activity of anti-aeromonas salmonicida antibodies after inactivation of complement in rainbow trout. | young rainbow trout (oncorhynchus mykiss) were immunized with killed aeromonas salmonicida, bled 5 wk later, and tested for the presence of serum agglutinins. the positive antisera were pooled and fractionated in a ultrogel aca 34 column. seven fractions were collected and concentrated. the higher agglutinin titres were found in the 2nd fraction, which also contained igm revealed by immunoelectrophoresis. the 7 fractions were heated for 30 min at 45 degrees c to inactivate the complement system, ... | 1991 | 2042906 |
| form and functions of the regular surface array (s-layer) of aeromonas salmonicida. | the principal virulence factor of aeromonas salmonicida is its s-layer (a-layer) which is comprised of tetragonally arrayed approximately 50,000 mr protein subunits tethered to the cell surface via lps. the detailed composition of its lps is known, as is the primary sequence, and three-dimensional disposition of the a protein subunits. the a-layer physically protects the cell against bacteriophage, proteases, as well as immune and non-immune complement. the a-layer appears to be uniquely adapted ... | 1991 | 2044685 |
| dna adducts in bronchial biopsies. | to investigate the feasibility of measuring dna-carcinogen adducts in the lungs of non-surgical patients, endobronchial biopsies were obtained from 78 patients undergoing routine diagnostic bronchoscopy. lung cancer was present in 37 (47%) of the patients. dna was isolated from the tissues and analyzed by hplc- or nuclease-pi-enriched 32p-postlabelling, using procedures selective for aromatic adducts. chromatograms from all 28 current smokers showed a distinctive diagonal adduct zone which was p ... | 2007 | 2045196 |
| cross resistance between oxytetracycline and oxolinic acid in aeromonas salmonicida associated with alterations in outer membrane proteins. | oxytetracycline resistant mutants of aeromonas salmonicida isolated from mutation frequency experiments showed decreased susceptibility to oxolinic acid. outer membrane preparations of these resistant mutant strains revealed a major protein, with a molecular mass of approximately 37 kda, which was not present in significant quantities in the parent strain. | 1990 | 2083844 |
| [aneurysm of the interatrial septum associated with lutembacher's syndrome. echocardiographic and doppler study]. | the case of a patient with suspected atrial septal defect is presented. echocardiographic-doppler study confirms the defect associated with mitral stenosis (lutembacher syndrome) and shows an atrial septal aneurysm through which the shunt is revealed. | 2007 | 2093966 |
| intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. | light and electron microscopy were used to analyze nine therapy-resistant and asymptomatic human periapical lesions, which were removed as block biopsies during surgical treatment of the affected teeth. the cases that required surgery represented about 10% of all of the cases which received endodontic treatment and root fillings during the period 1977 to 1984. these cases revealed periapical lesions when they were examined 4 to 10 yr after treatment. the biopsies were processed for correlated li ... | 1990 | 2094761 |
| aromatic-dependent mutants of aeromonas salmonicida. | 1990 | 2101485 | |
| [spectroscopy of the liver in vivo using nuclear magnetic resonance. a new approach in hepatic physiopathology]. | nuclear magnetic resonance (nmr) spectroscopy is a non-invasive means of detecting numerous compounds, thus enabling real-time studies to be carried out on such topics as energy metabolism, intracellular ph and main intermediate metabolism pathways. this method has been used for several years to study perfused livers and rat livers in vivo, but its application to man has just begun. we describe some of its principal uses, with emphasis on the possibilities it offers to investigate the metabolic ... | 1990 | 2140172 |
| restriction endonuclease fingerprinting analysis of canadian isolates of aeromonas salmonicida. | restriction endonuclease fingerprinting (ref) analysis was used to examine total cellular dna prepared from 56 independent field isolates of the fish pathogen, aeromonas salmonicida. dna was digested singly with the restriction enzymes ecori and hindiii, and the resulting fragments separated by polyacrylamide gel electrophoresis and visualized by silver staining. the ref patterns of typical isolates of a. salmonicida subsp. salmonicida were distinct from those of a. hydrophila, a. salmonicida su ... | 1990 | 2159364 |
| induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis. | 1990 | 2164982 | |
| reinforcement of colon anastomoses with polyglycolic acid mesh: an experimental study. | polyglycolic acid (pga) meshes have successfully been used in the treatment of injured parenchymatous organs. in our study we investigated the value of pga meshes for reinforcement of colon anastomoses in a rat model. in 75 wistar rats the transverse colon was transected and reanastomosed in a single-layer technique. the anastomoses in half of the animals were performed with 6 stitches. in the other animals the anastomoses were performed with 4 stitches only and were therefore supposed to leak. ... | 1990 | 2176159 |
| toxicology of selected pesticides, drugs, and chemicals. organophosphorus and carbamate insecticides. | organophosphorus and carbamate insecticides produce acute toxicosis via a common mechanism--the inhibition of acetylcholinesterase--and, therefore, these categories of insecticides are considered together. this article discusses the mechanisms, toxicity, clinical signs, lesions, and diagnosis of, as well as treatment for, toxicoses produced by these compounds. | 1990 | 2180181 |
| purification of cloned proaerolysin released by a low protease mutant of aeromonas salmonicida. | the precursor to the hole-forming toxin aerolysin has been purified in high yield from culture supernatants of a mutant of aeromonas salmonicida containing the cloned structural gene. the mutant strain was generated by tn5 mutagenesis. it released little or no protease or other extracellular proteins, including phospholipase, suggesting that it is a regulatory mutant. the absence of protease allowed the isolation of protoxin free from contaminating aerolysin. typically, more than 50 mg of pure p ... | 1990 | 2190617 |
| mutations in the trfa replication gene of the broad-host-range plasmid rk2 result in elevated plasmid copy numbers. | mutated forms of trfa, the replication protein gene of plasmid rk2, that support a minimal rk2 origin plasmid in escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. six such high-copy-number (copy-up) mutations were mapped and sequenced. in each case, a single base transition led to an amino acid substitution in the trfa protein primary sequence. the six mutations affected different residues of the protein and were located within a 69-base-pair region encoding 2 ... | 1990 | 2193921 |
| a concurrent increasing of natural antibodies and enhancement of resistance to furunculosis in rainbow trout. | in order to compare the respective roles of specific response and non-specific mechanisms in the enhancement of resistance to furunculosis, 2 groups of rainbow trout (oncorhynchus mykiss) were immunized with supernatants of aeromonas salmonicida or escherichia coli cultures. the fish received 4 weekly intraperitoneal injections (70 micrograms of crude proteins). one control group was injected with phosphate-buffered saline, while another was untreated. specific agglutinins were produced only in ... | 1990 | 2202250 |
| characterization of aeromonas salmonicida variants with altered cell surfaces and their use in studying surface protein assembly. | aeromonas salmonicida variants were characterized for alterations in their cell surface structure and used to examine reconstitution of the surface protein layer (a-layer). variants lacking outer membrane o-polysaccharide were devoid of a-layer and excreted stainable floret-like material of the surface protein (a-protein). one variant, showing partial loss of o-polysaccharide, was associated with a disrupted a-layer and excretion of some a-protein. variants lacking a-protein but possessing o-pol ... | 1990 | 2222123 |
| cytostatic and cytotoxic effects of recombinant tumor necrosis factor-alpha on sensitive human melanoma cells in vitro may result in selection of cells with enhanced markers of malignancy. | monolayer cultures of the human melanoma cell lines stml-12, stml-11, stml-14 (third, respectively, twenty-fifth subculture), and skmel-28 derived from specimens representing different stages of tumor progression were treated with 10-10,000 u/ml rtnf-alpha applied for 72 h. the effects of rtnf-alpha on cell proliferation, dna synthesis, cell viability, cloning efficiency, cell division, cell morphology, and the immunophenotype were studied in triplicate experiments. the cell line stml-14(3) reve ... | 1990 | 2258639 |
| cytostatic and cytotoxic effects of recombinant tumor necrosis factor-alpha on sensitive human melanoma cells in vitro may result in selection of cells with enhanced markers of malignancy. | monolayer cultures of the human melanoma cell lines stml-12, stml-11, stml-14 (third, respectively, twenty-fifth subculture), and skmel-28 derived from specimens representing different stages of tumor progression were treated with 10-10,000 u/ml rtnf-alpha applied for 72 h. the effects of rtnf-alpha on cell proliferation, dna synthesis, cell viability, cloning efficiency, cell division, cell morphology, and the immunophenotype were studied in triplicate experiments. the cell line stml-14(3) reve ... | 1990 | 2258639 |
| interfaces in medicine: asthma. report of a conference. | 2007 | 2258849 | |
| [duodenogastric reflux after organ-sparing operations in complicated duodenal ulcer]. | on the basis of treatment of 105 patients operated on for complicated duodenal ulcer disease, it was established that performance of the organ-preserving operation with the ulcer excision supplemented with the pylorus-restoring and pylorus-preserving intervention permitted to reduce considerably the incidence of duodenogastric reflux. | 2008 | 2259099 |
| purification and spectral study of a microbial fatty acyltransferase: activation by limited proteolysis. | a fatty acyltransferase with a reaction mechanism similar to that of mammalian lecithin: cholesterol acyltransferase has been purified from culture supernatants of a mutant aeromonas salmonicida containing the cloned aeromonas hydrophila structural gene. typically, more than 35 mg of protein were isolated from 2 l of culture supernatant. the amino-terminal sequence, amino acid composition, and molecular weight of the purified protein corresponded to predictions based on the sequence of the gene, ... | 1990 | 2271578 |
| in vitro activities of 4-quinolones against the fish pathogen aeromonas salmonicida. | the activities of five fluorinated 4-quinolones, namely, sarafloxacin, enrofloxacin, pd127391, pd117596, and ci934, against the fish pathogen aeromonas salmonicida were investigated and compared with that of oxolinic acid. the results indicated that with the exception of ci934, these drugs are more active than oxolinic acid in terms of mic. no inoculum effect was observed, but the drugs were less active at 10 degrees c than at 22 degrees c. the presence of 3% of nacl caused an increase in drug a ... | 1990 | 2285298 |
| [penis endoscopy: our experience apropos of 200 cases]. | the use of the colposcope and acetic acid in the man has shown many genital lesions due to papillomavirus which had not been revealed until then by clinical examination. the authors study the prevalence of these lesions in 200 partners of women with an hpv genital infection. 71.5 per cent abnormal peniscopies were found; 73.5 per cent in the group of partners of women presenting a simple viral disease and 63.5 per cent when the women has an associated cervical dysplasia. 34 per cent of the patie ... | 1990 | 2291046 |
| [principles of creating esophageal anastomoses]. | the techniques of formation of esophageal anastomoses are discussed on the basis of experience with 317 operations. the authors believe the following moments to be most important in the creation of communications with the esophagus: the use of a laser scalpel and monophilic atraumatic suture material, accurate apposition of the identical coats of the anastomosed organs by means of sutures placed at some distance from one another and tied not too tightly, creation of an antireflux mechanism in fo ... | 2008 | 2292863 |
| [features of oxygen supply in exercise load in patients with atherosclerosis obliterans of the peripheral arteries]. | the organism's oxygen balance was studied in 27 patients with atherosclerosis of the peripheral arteries during the test on a bicycle ergometer. the decrease in the patient's physical capacity was found to be in high correlation with the severity of oxygen disbalance. | 2008 | 2293156 |
| structural and chemical characterization of the s layer of a pseudomonas-like bacterium. | sections and freeze-fractured preparations showed an s layer on the surface of pseudomonas-like strain eu2. polyacrylamide gel electrophoresis of cell envelopes extracted with 1% sodium dodecyl sulfate (sds) at room temperature showed three proteins (45k, 55k, and 110k). the 55k protein was identified as the s-layer protein. incubation in 1.5 m guanidine hydrochloride removed the s layer from cell envelopes and dissociated the structure into subunits. the soluble 55k protein reassembled into pla ... | 1990 | 2298701 |
| digestibility of cotton plant by-product diets for sheep at two levels of intake. | crossbred wethers (n = 48) were assigned randomly to 1 of 12 experimental diets, arranged in a 3 x 2 x 2 split plot to evaluate three levels of cotton plant by-product (25, 50 and 75% cpb), two levels of naoh treatment of cpb (0 and 4%) and two levels of monensin supplementation (0 and 30 ppm, dm basis) at two intake levels (70 and 90% of ad libitum). intake was a within-lamb variable. ad libitum intake was established over an initial 17-d adjustment period. feed intake and fecal output were rec ... | 1990 | 2312420 |
| drop size and systemic adverse effects in timolol ophthalmic solution. | 2000 | 2327120 | |
| evidence against dormancy in the bacterial fish pathogen aeromonas salmonicida subsp. salmonicida. | results indicate that a. salmonicida does not enter an unculturable dormant state. the resuscitation of dormant cells by nutrient broth described by previous workers appears to be due to the presence of small numbers of viable, culturable cells too few to be detected by the sampling protocol employed. | 1990 | 2332152 |
| [arthroscopic surgery of the knee joint cartilage]. | the arthroscopic surgery of articular cartilage of the knee is beside conservative therapy and invasive surgical procedures an important enrichment in treatment of chondromalacia stage ii and iii. in the department of surgery of the municipal hospital of berlin-weissensee 3250 arthroscopic operations were performed between january 1983 and december 1988. in 227 patients chondromalacia stage ii and iii was found which had to be treated by arthroscopic surgery. four different operation methods wer ... | 2008 | 2386466 |
| using facs vs. communication scores to measure spontaneous facial expression of emotion in brain-damaged patients: a reply to mammucari et al. (1988). | buck and duffy (1980) and borod et al. (1985) found evidence of deficits in spontaneous expressiveness in right brain-damaged (rbd) patients relative to lbd patients and controls. using facs, mammucari et al. (1988) failed to replicate this result and questioned our methods and findings. this paper replies (a) that mammucari et al. (1988)'s review of our work is selective and misleading; (b) that there are aspects of their study that can account for their null results, including the insufficient ... | 2008 | 2387161 |
| glycerophospholipid:cholesterol acyltransferase complexed with lipopolysaccharide (lps) is a major lethal exotoxin and cytolysin of aeromonas salmonicida: lps stabilizes and enhances toxicity of the enzyme. | an extracellular lethal toxin produced by aeromonas salmonicida was purified by fast-protein liquid ion-exchange chromatography. the toxin is composed of glycerophospholipid:cholesterol acyltransferase (gcat) (molecular mass, 25 kilodaltons) aggregated with lipopolysaccharide (lps), the gcat/lps complex having a molecular mass of about 2,000 kilodaltons, estimated by gel filtration chromatography. the toxin is lethal for atlantic salmon (salmo salar l.) at a concentration of 0.045 micrograms of ... | 1990 | 2394687 |
| eosinophilic granular cells (egc) and histamine responses to aeromonas salmonicida toxins in rainbow trout. | the function of eosinophilic granular cells (egcs) in salmonids is unknown. in a previous study of the pathogenesis of a. salmonicida, injection of crude exotoxins into rainbow trout were shown to reproduce the lesions associated with furunculosis and an accompanying lesion, the dispersion and degranulation of egcs in the intestinal wall was reported. the present study investigated this phenomenon in relationship to the histamine content of the gut. in fish injected i/p with a. salmonicida exoto ... | 1985 | 2410306 |
| electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of aeromonas hydrophila. | sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides isolated from strains of aeromonas hydrophila which exhibit virulence for fish and which autoaggregate during growth in static broth culture. the lipopolysaccharides contained o-polysaccharide chains of homogeneous chain length. two of the strains produced a surface protein array, and immunofluorescence and phage-binding studies revealed that a number of these o-polysaccharide chains of homogene ... | 1985 | 2413008 |
| differential partition of virulent aeromonas salmonicida and attenuated derivatives possessing specific cell surface alterations in polymer aqueous-phase systems. | two-polymer aqueous-phase systems were used to compare via partitioning the surface properties of strains of the fish pathogen aeromonas salmonicida which differed in their ability to produce the surface protein array known as the a layer and in their ability to produce smooth lipopolysaccharide. in these two-phase systems, biological particles are known to partition between the phases in a manner related to a variety of surface properties, including hydrophobicity, charge, and lipid composition ... | 1986 | 2425736 |
| effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio. | the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ... | 1986 | 2429432 |
| the ontogeny of humoral immunity in rainbow trout, salmo gairdneri. | the ontogeny of the humoral immune response to a 'thymus dependent' and a 'thymus independent' antigen, human gamma globulin (hgg) and aeromonas salmonicida (as) respectively, was investigated in the fry of rainbow trout, salmo gairdneri, by direct immersion vaccination in the antigens (dose 5 mg/l hgg; 10(8) cells/ml as; 30 minutes) at known ages/weights from 7 days post hatch, and at 1, 2, 3, and 4 months post hatch. half the fry in each group were tested for antibodies 4 weeks after vaccinati ... | 1986 | 2429433 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |